From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!kristoff From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test of bionet.genome.chromosomes Message-ID: Date: 19 May 93 23:01:27 GMT Organization: BIOSCI International Newsgroups for Biology Lines: 2 test of bionet.genome.chromosomes From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!daresbury!daresbury!news From: kristoff@net.bio.net (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test of biochrom@daresbury.ac.uk Message-ID: <1993May19.225005.15694@gserv1.dl.ac.uk> Date: 19 May 93 22:47:51 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 2 Original-To: biochrom@uk.ac.daresbury test, please ignore. From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test of biochrom@net.bio.net Message-ID: Date: 19 May 93 22:50:19 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 2 another test From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test of biochrom@net.bio.net Message-ID: Date: 19 May 93 22:47:16 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 3 Test. This newsgroup is not yet operational. From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!kristoff From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test 2 of bionet.genome.chromosomes Message-ID: Date: 19 May 93 23:44:04 GMT Organization: BIOSCI International Newsgroups for Biology Lines: 2 test 2 bionet.genome.chromosomes From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!daresbury!daresbury!news From: kristoff@net.bio.net (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test 2 of biochrom@daresbury.ac.uk Message-ID: <1993May19.234251.16571@gserv1.dl.ac.uk> Date: 19 May 93 23:43:27 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 1 Original-To: biochrom@uk.ac.daresbury test 2 of biochrom@daresbury.ac.uk From owner-chromosomes@net.bio.net Tue May 18 23:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test 2 of biochrom@net.bio.net Message-ID: Date: 19 May 93 23:42:55 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 1 test 2 of biochrom@net.bio.net From owner-chromosomes@net.bio.net Thu May 20 23:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!mcsun!uunet!decwrl!pa.dec.com!nntpd2.cxo.dec.com!etre!wallace From: wallace@etre (Richard Wallace) Newsgroups: bionet.genome.chromosomes Subject: What is the purpose of this conference? Summary: What do we talk about Message-ID: <1993May21.141431.28025@nntpd2.cxo.dec.com> Date: 21 May 93 14:14:31 GMT Sender: usenet@nntpd2.cxo.dec.com (USENET News System) Organization: Digital Equipment Corporation Lines: 6 X-Newsreader: TIN [version 1.1 PL6] I'm hoping that this conference is going to be covering topics of current interest to researchers and persons who have a personal need for knowledge of current gene therapy. I am especially interested in chromosome 21 and Down syndrome. From owner-chromosomes@net.bio.net Thu May 20 23:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: CHROMOSOMES/bionet.genome.chromosomes ready for operation Message-ID: Date: 21 May 93 23:18:09 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 45 Hello everyone. We had a few glitches which required a bit more testing than usual, but the CHROMOSOMES/bionet.genome.chromosomes is ready to go. I invite Greg Lennon and Bruce Roe to post an introductory message about the group at their earliest possible convenience. First I should publicly acknowledge to my great embarrassment that I have forgotten how to count. Our e-mail addresses are supposed to be limited to 8 characters but somehow I let bio-chrom slip through during the voting process. We made a dictatorial decision in the interest of time to shorten the mailing addresses by deleting the hyphen, so e-mail users please post to either of the following: Posting Address Location --------------- -------- biochrom@daresbury.ac.uk Europe, Africa, and Central Asia biochrom@net.bio.net Americas and the Pacific Rim E-mail subscribers to the old CHROMOSOME-22 list have been added to the CHROMOSOMES mailing list. The same is true for e-mail subscribers to the autoseqs mailing list. Autoseqs was previously maintained solely at net.bio.net, but addresses for users in Europe, Africa, and Central Asia were sent to Daresbury for inclusion in the CHROMOSOMES group. Both the CHROMOSOME-22 group and autoseqs have been shut down. Mail sent to the old addresses will appear in the CHROMOSOMES newsgroup. If you have any questions about your e-mail subscription (e.g., autoseqs readers may want to cancel e-mail and use news to read bionet.genome.chromosomes if they have news access), please contact your regional biosci address below. Please note that it is sometimes difficult to determine the location of a user from their e-mail address (esp. for a few BITNET addresses), so our apologies if you might have been sent to the wrong site. Sincerely, Dave Kristofferson BIOSCI/bionet Manager kristoff@net.bio.net From owner-chromosomes@net.bio.net Thu May 20 23:00:00 1993 Path: biosci!daresbury!daresbury!news From: kristoff@net.bio.net (Dave Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: test of aliasing of chrom-22@daresbury.ac.uk Message-ID: <1993May21.224015.25483@gserv1.dl.ac.uk> Date: 21 May 93 22:40:43 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 2 Original-To: chrom-22@uk.ac.daresbury Test of alias of chrom-22@daresbury.ac.uk to biochrom@daresbury.ac.uk. From owner-chromosomes@net.bio.net Thu May 20 23:00:00 1993 Path: biosci!kristoff From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: Re: test 2 of bionet.genome.chromosomes Message-ID: Date: 21 May 93 22:04:35 GMT References: Organization: BIOSCI International Newsgroups for Biology Lines: 17 pdewerk@mondrian.CSUFresno.EDU (-Dragon-) writes: >kristoff@net.bio.net (David Kristofferson) writes: >: >: test 2 bionet.genome.chromosomes >Enough tests already! It passed!! ;-) Not quite 8-). I am checking our archiving system for one last possible glitch. Hope to open this group up momentarily. Sincerely, Dave Kristofferson BIOSCI/bionet Manager kristoff@net.bio.net From owner-chromosomes@net.bio.net Thu May 20 23:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!zaphod.mps.ohio-state.edu!usc!nic.csu.net!zimmer!news From: pdewerk@mondrian.CSUFresno.EDU (-Dragon-) Newsgroups: bionet.genome.chromosomes Subject: Re: test 2 of bionet.genome.chromosomes Message-ID: Date: 21 May 93 07:47:59 GMT References: Sender: news@zimmer.CSUFresno.EDU Organization: California State University, Fresno Lines: 12 Nntp-Posting-Host: mondrian.csufresno.edu kristoff@net.bio.net (David Kristofferson) writes: : : test 2 bionet.genome.chromosomes Enough tests already! It passed!! ;-) * - * - * Paul-Joseph "Dragon" de Werk \ Carpe diem. Dept. of Computer Science \ "First things first, but not California State Univ., Fresno \ necessarily in that order." paul-joseph_de_werk@CSUFresno.EDU \ -- The Doctor, "Dr. Who" From owner-chromosomes@net.bio.net Fri May 21 23:00:00 1993 Path: biosci!MED.PITT.EDU!rapr From: rapr@MED.PITT.EDU (Robert Preston) Newsgroups: bionet.genome.chromosomes Subject: 7pter markers Message-ID: <9305220338.AA15029@miranda.med.pitt.edu> Date: 22 May 93 03:38:15 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 Netpeople, does anyone know whether there are any mapped microsatellite loci more terminal than the D7S531 marker? Or, if not, whether any VNTRs have been mapped near 7pter? Thanks in advance, Robert Preston rapr@med.pitt.edu From owner-chromosomes@net.bio.net Fri May 21 23:00:00 1993 Path: biosci!imb.lan.nrc.ca!Mark From: Mark@imb.lan.nrc.ca Newsgroups: bionet.genome.chromosomes Subject: Cancel my subscription Message-ID: <2BFE6A87@coursmtp.nrc.ca> Date: 22 May 93 16:08:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 6 Please cancel my subscription. Mark Ragan, NRC-IMB (former autoseqs subscriber who couldn't care less about chromosome stuff) From owner-chromosomes@net.bio.net Mon May 24 23:00:00 1993 Path: biosci!ALCHEMY.CHEM.UTORONTO.CA!fbignone From: fbignone@ALCHEMY.CHEM.UTORONTO.CA (Franco Bignone) Newsgroups: bionet.genome.chromosomes Subject: beginning? Message-ID: <9305250427.AA05512@alchemy.chem.utoronto.ca> Date: 25 May 93 04:27:34 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 5 Well the newsgroup is brand new .... let me post a question. Does anybody know what is the state of the art with chromosome banding? What are the current ideas about the phaenomenon ... I am updated to 1984 .... more or less ... Franco From owner-chromosomes@net.bio.net Mon May 24 23:00:00 1993 Path: biosci!AARDVARK.UCS.UOKNOR.EDU!BROE From: BROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe) Newsgroups: bionet.genome.chromosomes Subject: Welcome to BIOCHROM Message-ID: <9305251151.AA26762@net.bio.net> Date: 25 May 93 11:53:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 56 Welcome to the CHROMOSOMES news group. Both of us are glad David finally got rid of the hyphen in bio-chrom and all is working. Our appologies for all the testing but ....... To post messages to the bionet.genome.chromosomes UseNet News Group via e-mail use the following address depending on your location: biochrom@daresbury.ac.uk from Europe, Africa, and Central Asia biochrom@net.bio.net from Americas and the Pacific Rim Or read, reply, post, etc via a UseNet News Reader program. As stated in our charter, included below, the purpose of this news group is to discuss topics related to Mapping and Sequencing Eucaryote Chromosomes. Both of us hope this will be a useful news group and serve as a mechanism for rapid communication for the genome community. We look forward to your questions, responses and discussions. Charter for bionet.genome.chromosomes ------------------------------------- Name: biochrom MAPPING AND SEQUENCING OF EUCARYOTE CHROMOSOMES Discussion Leaders: Greg Lennon and Bruce Roe The topics open for discussion include: 1. Physical mapping of human, mouse and other eucaryote chromosomes 2. Large-scale sequencing of cDNAs and chromosomes 3. Mapping of human, mouse and other eucaryote cDNAs to chromosomes 4. Selection of chromosome-specific cDNAs 5. Generation of chromosome-specific clones, sequences and probes. (STSs, microsatellites, etc.) with an emphasis on theory, techniques and discoveries. The overall purpose of this news group is to bring research groups with an interest in genomic-level research and automated methods for studying eucaryote genomes together to exchange ideas and protocols, probes and mapping information, and to discuss other issues relevant to mapping and sequencing eucaryote chromosomes, cDNAs and genes. Greg Lennon Human Genome Center, L-452 Lawrence Livermore National Laboratory Livermore, CA 94550 greg@mendel.llnl.gov Bruce A. Roe Department of Chemistry and Biochemistry University of Oklahoma 620 Parrington Oval Norman, Oklahoma 73019 broe@aardvark.ucs.uoknor.edu From owner-chromosomes@net.bio.net Mon May 24 23:00:00 1993 Path: biosci!uwm.edu!cs.utexas.edu!uunet!pipex!uknet!pavo.csi.cam.ac.uk!mrc-lmb.cam.ac.uk!njw From: njw@mrc-lmb.cam.ac.uk (White Nick) Newsgroups: bionet.genome.chromosomes Subject: Cell preparation for PCR Message-ID: <1993May25.140325.24866@infodev.cam.ac.uk> Date: 25 May 93 14:03:25 GMT Sender: news@infodev.cam.ac.uk (USENET news) Organization: MRC Laboratory of Molecular Biology, Cambridge UK Lines: 23 Nntp-Posting-Host: al.mrc-lmb.cam.ac.uk Hello! I'm assuming this group is now up and running - if it's not, "sorry". I'm working on chromosome twenty deletions associated with myeloproliferative disorders. My particular problem at present is I have one patient with what appears to be an unusual lineage involvement. Tomorrow (aaaagh) I'll be getting a new sample and flow sorting several leucocyte subsets.So far no problem but does anyone have a good reliable method for obtaining template DNA from a small number of cells (approx. 1000)? If replies could be mailed or even faxed directly to me I would be extremely grateful. Many Thanks Nick Nick J. White Telephone +44 223 330561 Department of Haematology Fax +44 223 336827 University of Cambridge E-Mail njw@mrc-lmb.cam.ac.uk MRC Laboratory of Molecular Biology Hills Road Cambridge UK CB2 2QH From owner-chromosomes@net.bio.net Mon May 24 23:00:00 1993 Path: biosci!MENDEL.LLNL.GOV!greg From: greg@MENDEL.LLNL.GOV Newsgroups: bionet.genome.chromosomes Subject: Re: Cell preparation for PCR Message-ID: <9305251715.AA00721@mendel.llnl.gov.> Date: 25 May 93 17:15:45 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 20 The simplest prep is probably just adding TE, and boiling for 10 minutes. Many folks then do a "prep-PCR" for 10-20 cycles using random primers, then use aliquots or dilutions of that PCR for the specific PCRs to follow. A few references on this or other methods for single cell PCR : NAR 21,1321-2 (1993) Genomics 13,718-725 (1992) Genomics 16,41-44 (1993) Genomics 10,748-755 (1991) Hope this helps, Greg Lennon Human Genome Center, L-452 Lawrence Livermore National Laboratory Livermore, CA 94550 USA From owner-chromosomes@net.bio.net Mon May 24 23:00:00 1993 Path: biosci!agate!howland.reston.ans.net!wupost!waikato.ac.nz!canterbury.ac.nz!chmeds.ac.nz!mkennedy From: mkennedy@chmeds.ac.nz (Martin Kennedy) Newsgroups: bionet.genome.chromosomes Subject: CA microsatellite repeat PCR Message-ID: <1993May26.103136.247@chmeds.ac.nz> Date: 25 May 93 22:31:36 GMT Lines: 38 Hi, A methods question for all you CA-repeat guru's: during the structural analysis of a gene on 1q32-1q42 I tripped over a CA repeat, and thought it worth working it up as a possibly informative STS. We first used one gamma-labelled primer on a handful of genomic DNAs, and ran out the products on a sequencing gel. The products were of the appropriate sizes, but horribly smeared - ie no definition of individual bands. We denatured them carefully in formamide dye mix etc, and didn't use intensifier screens on exposure. Does anyone have any idea what may have caused the smearing? Second problem is that we switched to direct incorporation of 35 S dATP, using 2uM dCTP,dGTP,TTP, and 0.2uM dATP, with 25 cycles of PCR. This time we got nicely resolvable products, but only in about 50% of the genomic DNA samples tested - we have titrated MgCl2 and it doesn't make much difference. We're about to repeat it all with direct incorporation of 32 P dCTP, and bumping up the cycles to 30, but I thought it wise to seek some advice from the experts. Any idea why the reactions are incocnsistent? (all the genomic DNAs we're using have been fine on Southern blots, so the quality should be ok). Is it better to keep the dNTP concentrations the same, say at 2uM, rather than reducing the conc of the cold dNTP equivalent to the labelled one? Is there a consensus "favoured" method for CA repeat amplification, or does everyone use a different variant? Thanks for your help, -- Cheers, Martin NNNN NN Martin A Kennedy (E-mail = mkennedy@chmeds.ac.nz) ZZZZZZZ NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ NN NN NN Christchurch School of Medicine ZZZ NN NNNN Christchurch, New Zealand ZZZZZZZ From owner-chromosomes@net.bio.net Tue May 25 23:00:00 1993 Path: biosci!enterpoop.mit.edu!senator-bedfellow.mit.edu!math.mit.edu!drw From: drw@euler.mit.edu (Dale R. Worley) Newsgroups: bionet.genome.chromosomes Subject: Chromosome band identification Message-ID: Date: 26 May 93 17:57:11 GMT Organization: MIT Dept. of Tetrapilotomy, Cambridge, MA, USA Lines: 15 NNTP-Posting-Host: euler.mit.edu I'm not a biologist, but I've always been curious what the band identifications like "1q32" are assigned. I know that the initial digits are the chromosome number, and the next character is p or q to identify the two arms. But how are the remaining digits assigned? All I've been able to figure out is that they are assigned in increasing order as you go out from the centromere. Thanks, Dale Dale Worley Dept. of Math., MIT drw@math.mit.edu -- Obviously, when you see someone go berserk and get a weapon and go in and murder people, of course, of course, it troubles me. -- George Bush From owner-chromosomes@net.bio.net Wed May 26 23:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!spool.mu.edu!torn!nott!nrcnet0!biologysx.lan.nrc.ca!hunter From: hunter@biologysx.lan.nrc.ca (David Hunter) Newsgroups: bionet.genome.chromosomes Subject: Re: What is the purpose of this conference? Summary: What do we talk about Message-ID: Date: 27 May 93 11:53:48 GMT References: <1993May21.141431.28025@nntpd2.cxo.dec.com> Sender: root@nrcnet0.nrc.ca (Operator) Organization: National Research Council of Canada Lines: 11 Nntp-Posting-Host: 132.246.164.58 In article <1993May21.141431.28025@nntpd2.cxo.dec.com> wallace@etre (Richard Wallace) writes: >From: wallace@etre (Richard Wallace) >Subject: What is the purpose of this conference? >Summary: What do we talk about >Date: Fri, 21 May 1993 14:14:31 GMT > >I'm hoping that this conference is going to be covering topics of current >interest to researchers and persons who have a personal need for >knowledge of current gene therapy. I am especially interested in >chromosome 21 and Down syndrome. From owner-chromosomes@net.bio.net Thu May 27 23:00:00 1993 Path: biosci!daresbury!daresbury!not-for-mail From: mbkxb@s-crim1.dl.ac.uk (K.C. Baker) Newsgroups: bionet.genome.chromosomes Subject: Du Pont Genesis 2000 Message-ID: <1u5aj4INNnbm@s-crim1.dl.ac.uk> Date: 28 May 93 15:20:04 GMT Organization: Daresbury Lab., Warrington, U.K. Lines: 13 NNTP-Posting-Host: s-crim1.dl.ac.uk We would like to contact other users of the Du Pont Genesis 2000 DNA sequencer. If you operate such a machine will you please contact me either here or (preferably) at BAKERK@UK.AC.AFRC.FRIR Many thanks Ken -- Dr Ken Baker JANET : UK.AC.DL.SEQNET::MBKXB Department of Protein Engineering INTERNET : MBKXB@SEQNET.DL.AC.UK AFRC Institute of Food Research TEL : (+44) 734 357139 Reading Berks RG6 2EF FAX : (+44) 734 267917 From owner-chromosomes@net.bio.net Sun May 30 23:00:00 1993 Path: biosci!enterpoop.mit.edu!gatech!howland.reston.ans.net!math.ohio-state.edu!cs.utexas.edu!tamsun.tamu.edu!rigel.tamu.edu!pkr1674 From: pkr1674@rigel.tamu.edu (PENNY RIGGS) Newsgroups: bionet.genome.chromosomes Subject: PiGMaP database Message-ID: <31MAY199312043885@rigel.tamu.edu> Date: 31 May 93 17:04:00 GMT Organization: Texas A&M University, Academic Computing Services Lines: 9 NNTP-Posting-Host: rigel.tamu.edu News-Software: VAX/VMS VNEWS 1.41 I was just curious about the status of the pig genome database in conjunction with the European PiGMaP. Is the the database "up and running," and if so, can it be accessed from the internet? -- Penny K. Riggs Department of Veterinary Pathobiology, pkr1674@zeus.tamu.edu Texas A&M Univ., College Station, TX 77843 pkr1674@tamzeus From owner-chromosomes@net.bio.net Sun May 30 23:00:00 1993 Path: biosci!uwm.edu!spool.mu.edu!uunet!pipex!uknet!comlab.ox.ac.uk!09p2 From: 09p2@bioch.ox.ac.uk (Part II #09 (Geoff Bowers)) Newsgroups: bionet.genome.chromosomes Subject: discs large gene - Drosophila Message-ID: <1993May31.175119.24541@newton.bioch.ox.ac.uk> Date: 31 May 93 16:51:19 GMT Distribution: bionet.genome.chromosomes Organization: Department of Biochemistry, University of Oxford Lines: 12 Originator: 09p2@newton.bioch I am looking for information on this gene - anyone out there have a recent reference on either its location and/or sequence. Failing that does anyone know what the exact spelling is? I can't seem to pick the damn thing up in Med-line!! Perhaps it is to recent... Many thanks... Geoff 09p2@bioch.ox.ac.uk MODIUS@vax.ox.ac.uk