From owner-chromosomes@net.bio.net Thu Jul 01 23:00:00 1993 Path: biosci!parc!decwrl!ames!haven.umd.edu!uunet!dziuxsolim.rutgers.edu!home.rutgers.edu!rhom From: rhom@home.rutgers.edu (Richie Hom) Newsgroups: bionet.genome.chromosomes Subject: Promoters Keywords: DNA, Promoters Message-ID: Date: 2 Jul 93 13:28:12 GMT Organization: Rutgers Univ., New Brunswick, N.J. Lines: 15 Hello I am new to this group and I don't know if this is the right place to post this. Currently I am working on a project in which I am trying to develop a very efficient way of identifing promoters in DNA which can lead to trying to identify introns and extrons by just look at a seqence. I was wondering if someone might have the new list of promoters on file that was published in this year issue 7 Nucleic Acids Research journal, the two copies in the libraries have both been sent to the binder and wont be back till later this month, and unfortunately I am a little data limited. Thanks in advance, Rich Hom rhom@dimacs.rutgers.edu _______________________________________________________________________ Just an undergrad looking for a place in this world. Research world that is. From owner-chromosomes@net.bio.net Thu Jul 01 23:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!dziuxsolim.rutgers.edu!home.rutgers.edu!rhom From: rhom@home.rutgers.edu (Richie Hom) Newsgroups: bionet.genome.chromosomes Subject: Sorry e-mail address Message-ID: Date: 2 Jul 93 13:34:19 GMT Organization: Rutgers Univ., New Brunswick, N.J. Lines: 10 I think I posted my wrong e-mail address It should be: rhom@dimacs.rutgers.edu And if anyine can tell me where the FTP sites for sequences of promters that would be great. Thanks again, Rich Hom From owner-chromosomes@net.bio.net Thu Jul 08 23:00:00 1993 Path: biosci!daresbury!buzz.bmc.uu.se!corax.udac.uu.se!sunic!uunet!bloom-beacon.mit.edu!genome.wi.mit.edu!lstein From: lstein@genome.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.molbio.gene-linkage,bionet.genome.chromosomes,bionet.software Subject: ANNOUNCING RELEASE 3 OF WI/MIT CGR MOUSE GENETIC MAP Keywords: SSLP genetic map linkage genome center mouse mus Message-ID: <21k03u$i6h@harpo.wi.mit.edu> Date: 9 Jul 93 14:42:06 GMT Expires: 1 Aug 1993 00:00:01 GMT Organization: Whitehead Institute for Biomedical Research Lines: 65 Xref: biosci bionet.molbio.gene-linkage:195 bionet.genome.chromosomes:53 bionet.software:5405 NNTP-Posting-Host: loco.wi.mit.edu ANNOUNCING THE THIRD RELEASE OF THE WHITEHEAD INSTITUTE/MIT GENOME CENTER GENETIC MAP OF THE MOUSE Release Three of the Whitehead Institute/MIT Genome Center Genetic Map of the Mouse is now available. This map consists of randomly-chosen simple sequence length polymorphisms (microsatellites) that can be analyzed using the polymerase chain reaction, as described in Dietrich, W., et al., Genetics 131:423-447 (1992). Currently the released map contains 1524 markers. The markers fall into 20 linkage groups spanning approximately 1400 cM with an average spacing of less than 1 cM. This data can be obtained in two different ways. 1. Via anonymous ftp to genome.wi.mit.edu. Log in as "anonymous" and use your e-mail address as password. The release can be found in the directory /distribution/mouse_sslp_release/july93/. The file "README" describes the file format and gives other information about the map. 2. Via internet e-mail using a database e-mail server. Using this service you can obtain locus and assay names of mapped SSLPs, the forward and reverse primer sequences, the genotypes of the loci on the mapping cross, the sizes of the PCR products on selected standard inbred strains, and other useful information. The database can be queried for markers meeting a number of different criteria. For example, you can ask for markers by name, by chromosome, or by position on the map. You can even request a list of markers that are polymorphic between two mouse strains. To obtain copies of the most current query forms, send a message to "genome_database@genome.wi.mit.edu" with either a subject line or body text of "help". You will receive instructions and a query form by return mail. Just fill out the form, send it to "genome_database@genome.wi.mit.edu", and the answer to your query will be mailed back automatically. This project is an ongoing one. As new markers are added to the map they will be released on a quarterly basis on approximately the following schedule: 1 October 1993 1 January 1994 1 April 1994 1 July 1994 &c. Please address questions and comments to me at the address below. Lincoln Stein ======================================================================== Lincoln Stein, M.D.,Ph.D. lstein@genome.wi.mit.edu MIT Genome Center (617) 252-1916 Whitehead Institute for Biomedical Research (617) 252-1902 FAX 9 Cambridge Center Cambridge, MA 02142 ======================================================================== -- ======================================================================== Lincoln D. Stein lstein@genome.wi.mit.edu Whitehead Institute, Cambridge, MA ======================================================================== From owner-chromosomes@net.bio.net Thu Jul 08 23:00:00 1993 Path: biosci!harpo.wi.mit.edu From: lstein@harpo.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.announce,bionet.genome.chromosomes,bionet.software,bionet.molbio.gene-linkage Subject: ANNOUNCING RELEASE 3 OF WI/MIT CGR MOUSE GENETIC MAP Keywords: SSLP genetic map linkage genome center mouse mus Message-ID: <21jv8f$hlt@harpo.wi.mit.edu> Date: 9 Jul 93 14:27:27 GMT Sender: kristoff@net.bio.net Followup-To: lstein@genome.wi.mit.edu Organization: Whitehead Institute for Biomedical Research Lines: 66 Approved: bionews-moderator@net.bio.net Xref: biosci bionet.announce:617 bionet.genome.chromosomes:54 bionet.software:5407 bionet.molbio.gene-linkage:196 ANNOUNCING THE THIRD RELEASE OF THE WHITEHEAD INSTITUTE/MIT GENOME CENTER GENETIC MAP OF THE MOUSE Release Three of the Whitehead Institute/MIT Genome Center Genetic Map of the Mouse is now available. This map consists of randomly-chosen simple sequence length polymorphisms (microsatellites) that can be analyzed using the polymerase chain reaction, as described in Dietrich, W., et al., Genetics 131:423-447 (1992). Currently the released map contains 1524 markers. The markers fall into 20 linkage groups spanning approximately 1400 cM with an average spacing of less than 1 cM. This data can be obtained in two different ways. 1. Via anonymous ftp to genome.wi.mit.edu. Log in as "anonymous" and use your e-mail address as password. The release can be found in the directory /distribution/mouse_sslp_release/july93/. The file "README" describes the file format and gives other information about the map. 2. Via internet e-mail using a database e-mail server. Using this service you can obtain locus and assay names of mapped SSLPs, the forward and reverse primer sequences, the genotypes of the loci on the mapping cross, the sizes of the PCR products on selected standard inbred strains, and other useful information. The database can be queried for markers meeting a number of different criteria. For example, you can ask for markers by name, by chromosome, or by position on the map. You can even request a list of markers that are polymorphic between two mouse strains. To obtain copies of the most current query forms, send a message to "genome_database@genome.wi.mit.edu" with either a subject line or body text of "help". You will receive instructions and a query form by return mail. Just fill out the form, send it to "genome_database@genome.wi.mit.edu", and the answer to your query will be mailed back automatically. This project is an ongoing one. As new markers are added to the map they will be released on a quarterly basis on approximately the following schedule: 1 October 1993 1 January 1994 1 April 1994 1 July 1994 &c. Please address questions and comments to me at the address below. Lincoln Stein ======================================================================== Lincoln Stein, M.D.,Ph.D. lstein@genome.wi.mit.edu MIT Genome Center (617) 252-1916 Whitehead Institute for Biomedical Research (617) 252-1902 FAX 9 Cambridge Center Cambridge, MA 02142 ======================================================================== -- ======================================================================== Lincoln D. Stein lstein@genome.wi.mit.edu Whitehead Institute, Cambridge, MA ======================================================================== From owner-chromosomes@net.bio.net Thu Jul 22 23:00:00 1993 Path: biosci!GENOME1.PED-GEN.UIOWA.EDU!ssunden From: ssunden@GENOME1.PED-GEN.UIOWA.EDU Newsgroups: bionet.genome.chromosomes Subject: comparative mapping publication Message-ID: <9307231600.AA07761@genome1.ped-gen.uiowa.edu> Date: 23 Jul 93 15:00:06 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 7 Does anyone know the title/author of a publication by academic press about comparative mapping in mammals? I've heard they have or are coming out with one, but I haven't been able to locate it. Thanks, Sara L.F. Sunden From owner-chromosomes@net.bio.net Thu Jul 22 23:00:00 1993 Path: biosci!fcircus.sat.tx.us!Jason_Burrell From: Jason_Burrell@fcircus.sat.tx.us (Jason Burrell) Newsgroups: bionet.genome.chromosomes Subject: AIDS virus Message-ID: <9307230505.AA01f9f@fcircus.sat.tx.us> Date: 23 Jul 93 05:05:34 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 16 There's probably a 50-50 chance that this is where this message belongs :) Is there anykind of DNA listing, in full or in part, of the AIDS virus? I figure that if its available, its probably somewhere on a FTP site, but I have no idea where to even begin looking. I realize that the file would be quite large. Any help on anything concerning this would be appreciated. --Jason R. Burrell -- Jason_Burrell@fcircus.sat.tx.us Device Driver Stacker.sys not found. Hit (P) to panic! -- PGP public key available upon request. From owner-chromosomes@net.bio.net Sun Jul 25 23:00:00 1993 Path: biosci!agate!howland.reston.ans.net!noc.near.net!das-news.harvard.edu!husc-news.harvard.edu!husc.harvard.edu!robison1 From: robison1@husc10.harvard.edu (Keith Robison) Newsgroups: bionet.genome.chromosomes Subject: Re: AIDS virus Message-ID: Date: 26 Jul 93 12:58:53 GMT References: <9307230505.AA01f9f@fcircus.sat.tx.us> Distribution: bionet Lines: 22 Nntp-Posting-Host: husc10.harvard.edu Many different isolates of HIV have been completely sequenced. To get the notorious BRU isolate, send the following message to retrieve@ncbi.nlm.nih.gov: datalib genbank maxlines 100000 begin HIVBRUCG [LOC] If you wish to explore this mail server further, send a message containing only the word "help" in the message body to the same address. Hope it helps Keith Robison Harvard University Department of Cellular and Developmental Biology Department of Genetics / HHMI robison@biosun.harvard.edu From owner-chromosomes@net.bio.net Wed Jul 28 23:00:00 1993 Path: biosci!ucsvc.ucs.unimelb.edu.au!U6065606 From: U6065606@ucsvc.ucs.unimelb.edu.au Newsgroups: bionet.genome.chromosomes Subject: P1 vector? Message-ID: <01H1487HR5O28WXLW0@ucsvc.ucs.unimelb.edu.au> Date: 29 Jul 93 04:01:03 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 10 I have heard that there is a plasmid -like vector able to carry inserts of about 100kb. It is apparently known as P1. Can anybody direct me to more information on this vector. Thanks Geoff McFadden Plant Cell Biology Research Centre School of Botany University of Melbourne, Australia From owner-chromosomes@net.bio.net Fri Jul 30 23:00:00 1993 Path: biosci!uwm.edu!cs.utexas.edu!swrinde!menudo.uh.edu!Elroy.UH.EDU!BCHS1B From: bchs1b@Elroy.UH.EDU (Michael Benedik) Newsgroups: bionet.genome.chromosomes Subject: Re: P1 vector? Message-ID: <23eha9$3od@menudo.uh.edu> Date: 31 Jul 93 19:31:21 GMT References: <01H1487HR5O28WXLW0@ucsvc.ucs.unimelb.edu.au> Reply-To: bchs1b@Elroy.UH.EDU Distribution: bionet Organization: University of Houston Lines: 20 NNTP-Posting-Host: elroy.uh.edu In article <01H1487HR5O28WXLW0@ucsvc.ucs.unimelb.edu.au>, U6065606@ucsvc.ucs.unimelb.edu.au writes: >I have heard that there is a plasmid -like vector able to carry >inserts of about 100kb. It is apparently known as P1. Can anybody >direct me to more information on this vector. > >Thanks > >Geoff McFadden >Plant Cell Biology Research Centre >School of Botany >University of Melbourne, Australia There is a packaging system available for phage P1, sort of like cosmids for lambda. I believe that NEN/Dupont is selling the appropriate kit. ---------------------------------------------------------------------- Michael Benedik INTERNET: Benedik@uh.edu Dept. of Biochemical & Biophysical Sciences University of Houston BITNET: Benedik@uhou -----------------------------------------------------------------------