From owner-chromosomes@net.bio.net Tue Nov 09 22:00:00 1993 Path: biosci!apeco.pe!jc From: jc@apeco.pe ("J.C. Riveros Salcedo") Newsgroups: bionet.genome.chromosomes Subject: Seabird_chromosomes Message-ID: <2cdfc961.apeco@apeco.pe> Date: 10 Nov 93 12:34:15 GMT Sender: daemon@net.bio.net Reply-To: "J.C. Riveros Salcedo" Distribution: bionet Organization: Asociacion Peruana para la Conservacion de la Naturaleza Lines: 8 I am interested in determine the sex ratio of selected species of seabirds at hatching, fledging and breeding stage. I have preliminary evidence (from external measurements-I can't sacrifice birds) that sex if biased towards males, but I need to know how the bias is distributed along age classes and how it is related to the life story of the species. Do you can point me to a reliable method of sex determination based on chromosome staining? Please respond to jc@apeco.pe Thanks. From owner-chromosomes@net.bio.net Wed Nov 10 22:00:00 1993 Path: biosci!ucsvc.ucs.unimelb.edu.au!U6065606 From: U6065606@ucsvc.ucs.unimelb.edu.au Newsgroups: bionet.genome.chromosomes Subject: Clone a whole chromosome? Message-ID: <01H575XX6A8Y8WWEN8@ucsvc.ucs.unimelb.edu.au> Date: 11 Nov 93 08:20:33 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 46 Dear Chromosome Group, We are investigating a small algal chromsome believed to carry genes involved in photosynthesis. The chromosome has an apparent size of 95kb in pulsed field gels. Would it be feasible to clone the entire chromosome into bacteriophage P1? We would welcome comments on the following strategy. If the ends of the 95kb chromosome are typical of eukaryotic chromosomes, they will contain telomeres with short 3' overhangs. These overhangs could be trimmed by the 3'-5' exonuclease activity of the T4 polymerase. Dephosphorylated BamH1 adaptors could then be ligated to the ends of the chromosome and the adaptors phosphorylated to allow the adaptor/chromosome complex to be ligated into the dephosphorylated site in the vector. We have chromosome-specific probes and STS PCR primers to screen for inserts. We envisage two possible DNA preps as start points. 1/ Cutting the 95kb band out of a pulsed field gel and using agarase to get the chromosome out intact. 2/ Alternatively we could use total high molecular weight genomic DNA (>200kb) from the algal cells. The size selection would then be impemented by the phage packaging system. Does anybody have any experience, opinions, pitfalls to avoid. Is this feasible in your experience. We keep telling ourself that we only need one clone, not a comprehensive library. Thanks Geoff McFadden and Paul Gilson Plant Cell Biology Research Centre University of Melbourne, Parkville 3052 VIC Australia _--_|\ email: u6065606@ucsvc.ucs.unimelb.edu.au / \ telephone: +61 3 344 5062 \_.--._/ fax: +61 3 347 1071 v From owner-chromosomes@net.bio.net Wed Nov 10 22:00:00 1993 Path: biosci!MENDEL.LLNL.GOV!greg From: greg@MENDEL.LLNL.GOV Newsgroups: bionet.genome.chromosomes Subject: Re: Clone a whole chromosome? Message-ID: <9311111921.AA03359@mendel.llnl.gov.> Date: 11 Nov 93 19:21:01 GMT Sender: daemon@net.bio.net Reply-To: Greg Lennon Distribution: bionet Lines: 65 You might want to think about cloning in a bacterial artificial chromosome (BAC) ; for a reference, see PNAS 89:8794 (1992). Greg Lennon Human Genome Center Lawrence Livermore National Laboratory > From BIOSCI-REQUEST@net.bio.net Wed Nov 10 23:36:11 1993 > Date: Thu, 11 Nov 1993 18:20:33 +1000 > From: U6065606@ucsvc.ucs.unimelb.edu.au > Subject: Clone a whole chromosome? > To: biochrom@net.bio.net > X-Vms-To: IN%"biochrom@net.bio.net" > Mime-Version: 1.0 > Content-Transfer-Encoding: 7BIT > Content-Length: 1738 > > Dear Chromosome Group, > > We are investigating a small algal chromsome believed to carry genes > involved in photosynthesis. The chromosome has an apparent size of > 95kb in pulsed field gels. Would it be feasible to clone the entire > chromosome into bacteriophage P1? We would welcome comments on the > following strategy. > > If the ends of the 95kb chromosome are typical of eukaryotic > chromosomes, they will contain telomeres with short 3' overhangs. > These overhangs could be trimmed by the 3'-5' exonuclease activity of > the T4 polymerase. Dephosphorylated BamH1 adaptors could then be > ligated to the ends of the chromosome and the adaptors phosphorylated > to allow the adaptor/chromosome complex to be ligated into the > dephosphorylated site in the vector. > > We have chromosome-specific probes and STS PCR primers to screen for > inserts. > > We envisage two possible DNA preps as start points. > > 1/ Cutting the 95kb band out of a pulsed field gel and using agarase > to get the chromosome out intact. > > 2/ Alternatively we could use total high molecular weight genomic > DNA (>200kb) from the algal cells. The size selection would then be > impemented by the phage packaging system. > > Does anybody have any experience, opinions, pitfalls to avoid. Is > this feasible in your experience. We keep telling ourself that we > only need one clone, not a comprehensive library. > > Thanks > > Geoff McFadden and Paul Gilson > Plant Cell Biology Research Centre > University of Melbourne, Parkville 3052 VIC > Australia > > _--_|\ email: u6065606@ucsvc.ucs.unimelb.edu.au > / \ telephone: +61 3 344 5062 > \_.--._/ fax: +61 3 347 1071 > v > > > > From owner-chromosomes@net.bio.net Fri Nov 12 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!spool.mu.edu!sgiblab!munnari.oz.au!metro!extro.ucc.su.OZ.AU!mayyar From: mayyar@extro.ucc.su.OZ.AU (Mohan Ayyar) Newsgroups: bionet.genome.chromosomes Subject: No. of Chromosones in large mammals? Message-ID: Date: 13 Nov 93 00:47:26 GMT Sender: news@ucc.su.OZ.AU Organization: Information Services, The University of Sydney, Sydney, NSW, Australia Lines: 11 Nntp-Posting-Host: extro.ucc.su.oz.au A friend of mine has asked me to find out how many chromosomes large mammals have. He is interested in animals like the elephant, hippo, rhino and whales. Any clues ? Email responses preferable. Mohan mayyar@extro.ucc.su.oz.au From owner-chromosomes@net.bio.net Fri Nov 12 22:00:00 1993 Path: biosci!GDB.ORG!rrobbins From: rrobbins@GDB.ORG (Robert Robbins) Newsgroups: bionet.genome.chromosomes Subject: Re: No. of Chromosones in large mammals? Message-ID: Date: 13 Nov 93 12:28:21 GMT References: <9311130848.AA10534@net.bio.net> Sender: daemon@net.bio.net Distribution: bionet Lines: 15 On 13 Nov 1993, Mohan Ayyar wrote: > A friend of mine has asked me to find out how many chromosomes large > mammals have. He is interested in animals like the elephant, hippo, > rhino and whales. Any clues? Email responses preferable. > > mayyar@extro.ucc.su.oz.au A similar, but more general query would be: Are there any on-line sources for comparative karyotypic data? If yes, an announcement to the net might be in order. From owner-chromosomes@net.bio.net Sun Nov 14 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!swrinde!emory!emory!usenet From: medtjm@bimcore.emory.edu (T. J. Murphy) Newsgroups: bionet.genome.chromosomes Subject: Test Message-ID: <2c8butINN2fi@emory.mathcs.emory.edu> Date: 15 Nov 93 16:49:33 GMT References: Reply-To: medtjm@bimcore.emory.edu Organization: Biomolecular Computing Resource, Emory University Lines: 1 NNTP-Posting-Host: bimcore.cc.emory.edu :Ignore From owner-chromosomes@net.bio.net Sun Nov 14 22:00:00 1993 Path: biosci!eeg.com!tim From: tim@eeg.com (Tim Stearns) Newsgroups: bionet.genome.chromosomes Subject: Re: No. of Chromosones in large mammals? Message-ID: <9311151617.AA04393@chaos.eeg.com> Date: 15 Nov 93 16:17:58 GMT References: Sender: daemon@net.bio.net Distribution: bionet Lines: 23 >On 13 Nov 1993, Mohan Ayyar wrote: > >> A friend of mine has asked me to find out how many chromosomes large >> mammals have. He is interested in animals like the elephant, hippo, >> rhino and whales. Any clues? Email responses preferable. >> >> mayyar@extro.ucc.su.oz.au > >A similar, but more general query would be: > > Are there any on-line sources for comparative karyotypic data? > >If yes, an announcement to the net might be in order. Mohan Ayyar and Dr. Robbins ask pose a very good question: where *can* one find cross-species karyotype data online? Tim Stearns EEG Systems Lab From owner-chromosomes@net.bio.net Wed Nov 17 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsserver.jvnc.net!newsserver.technet.sg!ntuix!ntuvax.ntu.ac.sg!asjose From: asjose@ntuvax.ntu.ac.sg Newsgroups: bionet.genome.chromosomes Subject: Test Message-ID: <1993Nov18.185017.1@ntuvax.ntu.ac.sg> Date: 18 Nov 93 10:50:17 GMT Sender: news@ntuix.ntu.ac.sg (USENET News System) Organization: Nanyang Technological University - Singapore Lines: 1 Nntp-Posting-Host: v7000.ntu.ac.sg Test: Ignore From owner-chromosomes@net.bio.net Wed Nov 17 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsserver.jvnc.net!newsserver.technet.sg!ntuix!ntuvax.ntu.ac.sg!asjose From: asjose@ntuvax.ntu.ac.sg Newsgroups: bionet.genome.chromosomes Subject: PhD Topic in Biotechnology Message-ID: <1993Nov18.185350.1@ntuvax.ntu.ac.sg> Date: 18 Nov 93 10:53:50 GMT Sender: news@ntuix.ntu.ac.sg (USENET News System) Organization: Nanyang Technological University - Singapore Lines: 7 Nntp-Posting-Host: v7000.ntu.ac.sg Hi Netters, My wife want to do her Ph.D in biotechnology. Can I get some good areas of research, pointers. Thanks in Advance, Jose K. Raphel E-mail: asjose@ntuvax.ntu.ac.sg From owner-chromosomes@net.bio.net Thu Nov 18 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!europa.eng.gtefsd.com!uunet!munnari.oz.au!ariel.ucs.unimelb.EDU.AU!ucsvc.ucs.unimelb.edu.au!lugb!NewsWatcher!user From: genmjw@lure.latrobe.edu.au (Matthew Wakefield) Newsgroups: bionet.genome.chromosomes Subject: Re: No. of Chromosones in large mammals? Message-ID: Date: 19 Nov 93 01:15:57 GMT References: <9311151617.AA04393@chaos.eeg.com> Sender: news@lugb.latrobe.edu.au (USENET News System) Followup-To: bionet.genome.chromosomes Distribution: bionet Organization: LaTrobe University, Australia Lines: 19 > >> A friend of mine has asked me to find out how many chromosomes large > >> mammals have. He is interested in animals like the elephant, hippo, > >> rhino and whales. Any clues? Email responses preferable. > >> mayyar@extro.ucc.su.oz.au > >A similar, but more general query would be: > > Are there any on-line sources for comparative karyotypic data? I tried a veronica search and it turned up zip. Eventually I would like to put this sort of data on the comparative mapping Gopher I am setting up. This should be up and running by Christmas, but with about 600 loci in 30 species it is taking a while to get this info onto the computer and into a useful format. I will post details in early December. If anyoune does have some comparative karyotypic data, such as digitised pictures of karyotypes or diagrams, please let me know. (=) Matthew Wakefield (=) Genetics and Human Variation (=) La Trobe University, Bundoora, VIC, AUSTRALIA (=) INTERNET GENMJW@LURE.LATROBE.EDU.AU From owner-chromosomes@net.bio.net Mon Nov 29 22:00:00 1993 Path: biosci!mrc-crc.ac.uk From: smercer@mrc-crc.ac.uk (Mr. S.J. Mercer) Newsgroups: bionet.announce,bionet.genome.chromosomes,bionet.molbio.gdb Subject: Chromosome Database available on Gopher Keywords: Chromosome Database Gopher Message-ID: <1993Nov30.145230.28881@crc.ac.uk> Date: 30 Nov 93 14:52:30 GMT Sender: kristoff@net.bio.net Followup-To: poster Organization: MRC Human Genome Resource Centre Lines: 31 Approved: bionews-moderator@net.bio.net Xref: biosci bionet.announce:814 bionet.genome.chromosomes:115 bionet.molbio.gdb:135 ANNOUNCEMENT The Chromosome Abnormality Database is now available by Gopher from the UK Human Genome Mapping Project Resource Centre (HGMP-RC). This database was established early in 1991,to collect records of chromsosome abnormalities (of all types) from the fifty or so laboratories in the United Kingdom which perform routine diagnostic karyotyping. Ordinarily, the records of abnormal samples were held separately by each laboratory, but the Chromosome Abnormality Database (CAD) acts as a centralised repository for this information and enabling researchers to locate samples of interest without having to contact all of the laboratories individually. The CAD now stores over forty thousand records of chromosome abnormality and for each record holds the sex, date of birth, tissue type and karyotype, as well as details of the lab from which the record was collected. It also holds information on the availability of stored samples and cell lines where these are available. Point your gopher at menu.hgmp.mrc.ac.uk port 70 or make a link: Name: Chromosome Abnormality Database Type: 1 Port: 70 Path: 1/HGMP_DBs/Cad_DB Host: menu.hgmp.mrc.ac.uk If you have any further questions, please email me at simon@bioch.ox.ac.uk. Applications for an account at the UK HGMP-RC should be made to cbates@hgmp.mrc.ac.uk Tel: +44 81 869 3446 Fax: +44 81 869 3807 From owner-chromosomes@net.bio.net Mon Nov 29 22:00:00 1993 Path: biosci!GALAXY.GOV.BC.CA!PMACLEOD From: PMACLEOD@GALAXY.GOV.BC.CA (Patrick Macleod 727-4461) Newsgroups: bionet.genome.chromosomes Subject: Chromosome 1 Message-ID: <01H5XAM1YNACB3VOKA@mr.gov.bc.ca> Date: 30 Nov 93 19:04:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 8 For the time being try : Nature genetics 3:Feb.1993 O'Brien J.O. et al Human Genetics January 1993 McKusick V. IN the near term look for Cytogenetics and Cell Genetics in press. It will have the latest update. From owner-chromosomes@net.bio.net Mon Nov 29 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!spool.mu.edu!sdd.hp.com!network.ucsd.edu!clinical-mac-95.ucsd.edu!user From: dkolk@popmail.ucsd.edu (Dan Kolk) Newsgroups: bionet.genome.chromosomes Subject: chromosome 1q Message-ID: Date: 30 Nov 93 12:34:31 GMT Followup-To: bionet.genome.chromosomes Organization: UCSD School of Medicine Lines: 7 NNTP-Posting-Host: clinical-mac-95.ucsd.edu Does anyone know where I can find the most up-to-date map of chromosome 1 and particular the 1q32 region. I need a map with references please. Thank you, Dan Kolk