From owner-chromosomes@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!library.ucla.edu!network.ucsd.edu!clinical-mac-95.ucsd.edu!user From: dkolk@popmail.ucsd.edu (Dan Kolk) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome 1q Message-ID: Date: 2 Dec 93 15:11:49 GMT Followup-To: bionet.genome.chromosomes Organization: UCSD School of Medicine Lines: 2 NNTP-Posting-Host: clinical-mac-95.ucsd.edu Thank to all who responded to my chromosome 1 request. I have registered with the GDB. From owner-chromosomes@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!pipex!howland.reston.ans.net!darwin.sura.net!news-feed-1.peachnet.edu!concert!rutgers!princeton!phoenix.Princeton.EDU!harnad From: harnad@phoenix.Princeton.EDU (Stevan Harnad) Newsgroups: bionet.neuroscience,bionet.cellbiol,bionet.molbio.ageing,bionet.genome.chromosomes Subject: Neural Transplantation: Call for Commentators Keywords: neural grafts, gene therapy, brain damage, functional recovery Message-ID: <1993Dec2.224215.21986@Princeton.EDU> Date: 2 Dec 93 22:42:15 GMT Sender: news@Princeton.EDU (USENET News System) Organization: Princeton University Lines: 222 Xref: biosci bionet.neuroscience:2127 bionet.cellbiol:197 bionet.molbio.ageing:578 bionet.genome.chromosomes:117 Originator: news@nimaster Nntp-Posting-Host: phoenix.princeton.edu BBS Special Issue CONTROVERSIES IN NEUROSCIENCE II: Neural Transplantation Below are the abstracts of 3 forthcoming target articles for a special issue on Neural Transplantation that will appear in Behavioral and Brain Sciences (BBS), an international, interdisciplinary journal that provides Open Peer Commentary on important and controversial current research in the biobehavioral and cognitive sciences. This will be the second in a new series called "Controversies in Neuroscience," undertaken in collaboration with Paul Cordo and the RS Dow Neurological Science Institute. Commentators must be current BBS Associates or nominated by a current BBS Associate. To be considered as a commentator on any of these articles, to suggest other appropriate commentators, or for information about how to become a BBS Associate, please send email to: harnad@clarity.princeton.edu or harnad@pucc.bitnet or write to: BBS, 20 Nassau Street, #240, Princeton NJ 08542 [tel: 609-921-7771] Please specify which article or articles you would like to comment on. (Commentators are allotted 1000 words to comment on one article, 1750 words to comment on two, and a maximum of 2250 words to comment on all three target articles.) To help us put together a balanced list of commentators, please give some indication of the aspects of the topic on which you would bring your areas of expertise to bear if you were selected as a commentator. Within the next week or so, electronic drafts of the full text of each article will be available for inspection by anonymous ftp, archie, gopher or versonica on host princeton.edu directory pub/harnad/BBS according to the instructions that follow after the abstracts. These drafts are for inspection only; please do not prepare a commentary until you are formally invited to do so. 1. NEURAL TRANSPLANTATION AND RECOVERY OF COGNITIVE FUNCTION John D. Sinden, Helen Hodges & Jeffrey A. Gray Filename: bbs.sinden 2. FETAL BRAIN TISSUE GRAFTS AS THERAPY FOR BRAIN DYSFUNCTIONS Donald G. Stein & Marylou M. Glasier Filename: bbs.stein 3. GENE REPLACEMENT THERAPY IN THE CENTRAL NERVOUS SYSTEM Edward A.Neuwelt, Michael A. Pagel, Leslie L. Muldoon & Alfred Geller Filename: bbs.neuwelt --------------------------------------------------------------------- 1. NEURAL TRANSPLANTATION AND RECOVERY OF COGNITIVE FUNCTION John D. Sinden, Helen Hodges & Jeffrey A. Gray Department of Psychology Institute of Psychiatry De Crespigny Park Denmark Hill London SE5 8AF England spjtjds@ucl.ac.uk jgray@ux.psych.lon.ac.uk KEYWORDS: Cholinergic system, cerebral ischaemia, cognitive function, neural grafts. ABSTRACT: Cognitive deficits were produced in rats using different methods of damaging the brain: chronic ingestion of alcohol, causing widespread damage to diffuse cholinergic and aminergic projection systems; lesions (by local injection of the excitotoxins, ibotenate, quisqualate and AMPA) to the nuclei of origin of the forebrain cholinergic projection system (FCPS), which innervates the neocortex and hippocampal formation; transient cerebral ischaemia, producing focal damage, especially in the CA1 pyramidal cells of the dorsal hippocampus; and lesions (by local injection of the neurotoxin, colchicine) to the granule cells of the dentrate gyrus. Following chronic alcohol or lesions of the FCPS, transplants of cholinergically rich fetal brain tissue into the terminal areas (neocortex or hippocampus) restored performance almost to control levels, with a time-course consistent with growth of the transplants and integration with host tissue; transplants of cholinergically poor fetal tissue (hippocampus) were without effect, as were transplants of cholinergically rich tissue into the region containing the nuclei of origin of the FCPS. Grafts of primary cells enriched in glia and cultured neuroblastoma cells into the terminal areas of the FCPS were equally effective, suggesting that there are multiple mechanisms by which neural transplants can restore cognitive function following diffuse cholinergic damage. In contrast, after ischaemia- or neurotoxin-induced damage to CA1 or dentate granule cells respectively, cholinergically rich fetal transplants into the damaged hippocampal formation were ineffective in restoring performance. However, after ischaemic damage, performance was restored by suspension grafts of CA1 cells but not by transplants containing CA3 pyramidal cells or granule cells; and after colchicine damage, performance was restored by solid grafts containing granule but not CA1 pyramidal cells. Furthermore, electrophysiological evidence has demonstrated functional, graft type-specific host-graft fuctional neuronal connectivity. Thus, restoration of cognitive function by neural transplants is possible after damage to either diffuse (cholinergic) or point-to-point (intrahippocampal) forebrain systems, but the transplant must be appropriate to the damage to be repaired. Since the different types of brain damage studies provide partial analogues of human alcoholic dementia, Alzheimer's disease and heart attack, these results are encouraging with regard to the eventual application of neural transplant surgery to the treatment of cognitive deficits in humans. ----------------------------------------------------------------------- 2. FETAL BRAIN TISSUE GRAFTS AS THERAPY FOR BRAIN DYSFUNCTIONS: SOME PRACTICAL AND THEORETICAL ISSUES Donald G. Stein & Marylou M. Glasier Laboratory of Brain Research and CNS Plasticity Institute of Animal Behavior Rutgers University Newark, NJ 07102 stein@draco.rutgers.edu KEYWORDS: Brain damage; functional recovery; grafts; neural grafts; neural transplants ABSTRACT: Grafting embryonic neural tissue into the brains of adult patients is currently being used to treat Parkinson's disease and is being given serious consideration as therapy for a variety of other degenerative and traumatic disorders. This target article evaluates the use of transplants to promote recovery from brain injury and highlights the kinds of questions and problems that must be addressed before this form of therapy is routinely applied. It has been argued that neural transplantation can promote functional recovery through the replacement of damaged nerve cells, the reestablishment of specific nerve pathways lost as a result of injury, the release of specific neurotransmitters, or the production of factors that promote neuronal growth. The latter two mechanisms, which need not rely on anatomical connections to the host brain, are open to examination through nonsurgical, less intrusive therapy. Subjective judgments in selecting which patients will receive grafts and in assessing the outcome of graft therapy make evaluation of the procedure methodologically difficult. In addition, little long-term assessment of transplant efficacy and effect has been done in nonhuman primates. Carefully controlled human studies, with multiple testing paradigms, are also needed to establish the efficacy of transplant therapy. ----------------------------------------------------------------------- 3. GENE REPLACEMENT THERAPY IN THE CENTRAL NERVOUS SYSTEM: VIRAL VECTOR MEDIATED THERAPY OF GLOBAL NEURODEGENERATIVE DISEASE Edward A.Neuwelt, Michael A. Pagel, Leslie L. Muldoon Oregon Health Sciences University, Portland OR 97201 Alred Geller Children's Hospital Boston, MA 02115 KEYWORDS: adenovirus; blood-brain barrier; gene therapy; herpes virus; pHexosaminidase ABSTRACT: This target article describes the current state of global gene replacement in the brain through the use of viral vectors and it assesses possible solutions to some of the many problems inherent in gene therapy for the central nervous system (CNS). Gene replacement therapy is a way to generate normal human proteins in deficient cells, making cures possible for certain genetically inherited enzyme deficiences, metabolic diseases, and cancers. The two major issues to be addressed are the delivery of genetic material to the brain and the expression of recombinant genetic material in CNS target cells. Focal inoculation of recombinant virions or other genetic vectors has limitations when there is global brain disease. A new blood-brain-barrier (BBB) disruption technique, in which hypertonic mannitol transiently shrinks the BBB endothelium, allows the passage of high molecular weight compounds and even viruses. CNS gene therapy will require a viral vector system that allows long-term, nontoxic gene expression in neurons or glial cells. Retroviral vectors have limitations in CNS gene replacement, although they are suitable for expressing recombinant genes in intracerebral grafts, or toxic genes in brain tumors. Mutant neurotropic viruses with reduced neurotoxicity (e.g., defective herpes simplex virus type 1 [HSV-1], the HSV-1 amplicon vector system we have developed, or adenovirus mutants) have potential for direct treatment of neurons. Injecting these vectors into rodent brains can lead to the stable expression of foreign genetic material in postmitotic neuronal cells. We discuss our BBB disruption delivery technique, our defective HSV-1 aplicon vector system, and our feline model for the neuronal lysosomal storage disorder Gm2-gangliosidosis (Sandhoff disease), which may prove to be a useful model system for CNS gene therapy. ---------------------------------------------------------------------- To help you decide whether you would be an appropriate commentator for this article, electronic drafts are retrievable by anonymous ftp from princeton.edu according to the instructions below (the filenames are bbs.sinden bbs.stein and bbs.neuwelt). Please do not prepare a commentary on these drafts. Just let us know, after having inspected them, what relevant expertise you feel you would bring to bear on what aspect of each article. ------------------------------------------------------------- To retrieve a file by ftp from a Unix/Internet site, type either: ftp princeton.edu or ftp 128.112.128.1 When you are asked for your login, type: anonymous Enter password as per instructions (make sure to include the specified @), and then change directories with: cd /pub/harnad/BBS To show the available files, type: ls Next, retrieve the file you want with (for example): get bbs.sinden When you have the file(s) you want, type: quit In case of doubt or difficulty, consult your system manager. A more elaborate version of these instructions for the U.K. is available on request (thanks to Brian Josephson). The files are also retrievable through archie, gopher, veronica, etc. ---------- Where the above procedures are not available (e.g. from Bitnet or other networks), there are two fileservers: ftpmail@decwrl.dec.com and bitftp@pucc.bitnet that will do the transfer for you. To one or the other of them, send the following one line message: help for instructions (which will be similar to the above, but will be in the form of a series of lines in an email message that ftpmail or bitftp will then execute for you). ------------------------------------------------------------- From owner-chromosomes@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.ecn.bgu.edu!anaxagoras.ils.nwu.edu!news.acns.nwu.edu!raven.alaska.edu!netnews.nwnet.net!news.u.washington.edu!carson.u.washington.edu!bethp From: bethp@carson.u.washington.edu (Beth Plotkin) Newsgroups: bionet.genome.chromosomes Subject: Genetic Diversity??? Summary: Genetic Diversity??? Keywords: genetic diversity Message-ID: <2e1dgk$k9j@news.u.washington.edu> Date: 7 Dec 93 08:05:40 GMT Organization: University of Washington Lines: 23 NNTP-Posting-Host: carson.u.washington.edu Here's a perhaps naive question from a non-genetics-type person: I know that incest has long been taboo, since the likelihood of genetic problems in off-spring increases in these situations. Is it preferable, therefore, for two parents to be as diverse genetically as possible (in a given species)? Some breeds of dogs, for example, have strange dispositions... yet many "mutts" often seem to be well-disposed. When you constantly breed for certain traits through the generations, aren't there apt to be more "problems" as well? If this were true with people, "inter-racial" marriages should be encouraged to prevent in-breeding. Unlike dogs, where traits are selected by people, our traits stem from our nomadic wanderings, do they not? We're all Homo sapiens, but blue eyes, brown skin were all preferred adaptations for different climates? Such racial purists as Adolph Hitler were complete oblivious! Sorry if this is an ignorant or offensive question to anyone. I don't read this net but am curious about this subject. From owner-chromosomes@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.genome.chromosomes Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312061945.AA20474@judy.eng.uci.edu> Date: 6 Dec 93 19:45:40 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-chromosomes@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.genome.chromosomes Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312060500.AA19369@judy.eng.uci.edu> Date: 6 Dec 93 05:00:44 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-chromosomes@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!MENDEL.LLNL.GOV!greg From: greg@MENDEL.LLNL.GOV Newsgroups: bionet.genome.chromosomes Subject: Human Chromosome Ideograms Message-ID: <9312071713.AA02683@mendel.llnl.gov> Date: 7 Dec 93 17:13:56 GMT Sender: daemon@net.bio.net Reply-To: Greg Lennon Distribution: bionet Lines: 26 As scooped off human-genome-program@net.bio.net, as posted by Uta Francke : TO: Human Cytogeneticists and Genome Mappers Human chromosome ideograms that depict 850 bands, numbered in agreement with ISCN (1981) nomenclature but based on actual measurements of band sizes and differentiated by five shades of staining intensity, have been digitized using ADOBE graphics software. These ideograms provide for more accurate estimates of distances within chromosome arms, DNA content of chromosome bands and correlation between cytohgenetic, molecular and genetic linkage maps than the standard ISCN ideograms. Reference: Francke, Uta (1994) Digitized and differentially shaded human chromosome ideograms for genomic application. Cytogenet. Cell Genet 65:206-218 Diskettes for Mac or IBM compatible computers are available from the publisher of CCG who has all the rights. For information: Editorial office, Cytogenetics and Cell Genetics, Dr. Harold P. Klinger, Phone: 718-430-2451 FAX: 718-430-2454 From owner-chromosomes@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!HELIX.MGH.HARVARD.EDU!HAINES From: HAINES@HELIX.MGH.HARVARD.EDU ("Jonathan L. Haines") Newsgroups: bionet.genome.chromosomes Subject: (none) Message-ID: <01H693TB88B68WY03F@HELIX.MGH.HARVARD.EDU> Date: 9 Dec 93 03:09:25 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 152 - COURSE ANNOUNCEMENT - GENETIC ANALYSIS METHODS FOR MEDICAL RESEARCHERS Description: A comprehensive four day course directed toward physician scientists and/or other medical researchers that will introduce them to state-of-the-art approaches for mapping human inherited disorders of both a monogenic and polygenic nature, such as cancer syndromes, multiple sclerosis, etc. The overall focus of the course is on the development of a broad-based knowledge of the resources available through the Human Genome Initiative, and overall project decision making, rather than the mechanics of specific techniques. The specific goals are: 1. To outline and instruct participants in detail about the necessary steps and procedures used in ascertaining and collecting pedigree data and family history information. These processes include the use of family history questionnaires, computer management systems, simulation of data to determine linkage power, designs for clinical sampling schemes, and the actual sampling and subsequent cataloguing and storing of pedigree DNA for study. 2. To discuss in detail the methodologies for using the Index Marker Maps generated by the efforts of the Human Genome Initiative. This will include a broad understanding of PCR technology and its use for genotyping dinucleotide or other simple sequence length polymorphisms. It will also include a discussion of statistical analytical methods such as lod scores and the examination of affected relative pairs. Emphasis will be on working examples as well as the basic theory behind these genetic mapping methodologies rather than intensive computational exercises. Information will be available about the computational resources necessary to undertake such a study and how to obtain them. 3. To educate participants on the general interpretation of linkage results. Discussions will include an overview of positional cloning strategies, refinement of the preliminary linkage data, examination of heterogeneity, physical mapping, cDNA analysis, exon amplification etc. This course will not include any bench or "wet" laboratory experience. 4. To incorporate discussion of the participant's individual research interests. Participants will be encouraged to bring preliminary information and/or data for both formal and informal group discussion and instructor consultation. Course participants will be polled prior to the course about their particular needs and interests and their own motivations for taking the course. Extensive efforts will be made to incorporate the suggested topics of participants. Faculty: Organizers: Margaret A. Pericak-Vance, Ph.D. Dept. of Medicine Division of Neurology Duke University Medical Center Jonathan L. Haines, Ph.D. Molecular Neurogenetics Unit Massachusetts General Hospital Instructors: Jeffrey Murray, M.D. Division of Medical Genetics University of Iowa Hospitals Marcy C. Speer, Ph.D. Dept. of Medicine Division of Neurology Duke University Medical Center Arthur S. Aylsworth, M.D. Chief, Division of Genetics and Metabolism, Dept. of Pediatrics University of North Carolina at Chapel Hill David Goldgar, Ph.D. Dept. of Medical Informatics University of Utah Deborah Meyers, Ph.D. Dept. of Medicine School of Medicine Johns Hopkins University The broad-based faculty has extensive expertise in genetic epidemiology, positional cloning, reference marker mapping, laboratory techniques, clinical diagnosis and family collection. Faculty will be available throughout the course for informal discussions. Location: The R. David Thomas Center The Fuqua School of Business Duke University Durham, NC The Thomas center, by offering both in-house accommodations and meals, promotes maximum interactions, both formal and informal, between participants. Most faculty will be staying on-site for the duration of the course. Date: May 15 through May 18, 1994 Participation: Individuals with an M.D. or Ph.D. and a specific disease-related interest are encouraged to apply but participation will be limited to a total of 35 individuals. Up to five scholarships will be available for M.D. and/or Ph.D. students and fellows. Women and minorities are specifically encouraged to apply. The deadline for receipt of completed application forms is February 21, 1994. For more information about registration fees, course requirements, etc: Write: Genetic Methods Course c/o Dr. Margaret Pericak-Vance Division of Neurology, Box 2900 Duke University Medical Center Durham, NC 27710 OR: E-Mail: genclass@genemap.mc.duke.edu In any correspondence, please include a postal address. From owner-chromosomes@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.genome.chromosomes Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312061945.AA20474@judy.eng.uci.edu> Date: 6 Dec 93 19:45:40 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-chromosomes@net.bio.net Thu Dec 09 22:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (David Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: IMPORTANT BIOSCI INFORMATION Message-ID: <9312101000.AA28283@net.bio.net> Date: 10 Dec 93 10:00:04 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 244 Three important items follow: BIOSCI archive searching by e-mail, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our e-mail address directory, please take a few minutes to complete and return the form below. If your address information has changed since you listed yourself, please send us an updated form. Sincerely, Dave Kristofferson BIOSCI/bionet Manager kristoff@net.bio.net **** SEARCHING BIOSCI ARCHIVES WITH WAISMAIL **** E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank. Other methods of searching the archives via WAIS and gopher are described in the BIOSCI FAQ. **** BIOSCI FREQUENTLY ASKED QUESTIONS (FAQ) SHEET **** New users of BIOSCI/bionet may want to read the "Frequently Asked Questions" or "FAQ" sheet for BIOSCI. The FAQ provides details on how to participate in these forums and is available for anonymous FTP from net.bio.net [134.172.2.69] in pub/BIOSCI/biosci.FAQ or for retrieval by gopher to net.bio.net, port 70. It may also be requested by sending e-mail to biosci@net.bio.net (use plain English for your request). The FAQ is also posted on the first of each month to the newsgroup BIONEWS/bionet.announce immediately following the posting of the BIOSCI information sheet. **** BIOSCI USER ADDRESS DIRECTORY **** Please take this opportunity to add your name and address information to the BIOSCI User Address Database if you have not already done so. Below is the address form that we would like each reader of the BIOSCI/bionet newsgroups to complete and return if you would like to be listed in our database. The database serves as a directory that enables biologists, who are currently using (or even just reading) the BIOSCI newsgroups, to look up e-mail addresses and other information about our users. The address database is reindexed nightly for WAIS and waismail access (waismail is our WAIS e-mail server, more below) and will also be available for access via other gopher sites if they wish to permit it. The raw unindexed data is available for FTP from net.bio.net and is atomized sufficiently to allow import into your local RDBMS should you so desire. Please carefully follow the instructions for completing the form below and return it to either of the following two addresses (whichever is more convenient for you). Thanks in advance for taking the time to complete and return the form. Addresses for returning forms Location Network ----------------------------- -------- ------- biovote@net.bio.net U.S.A. Internet/BITNET biovote@daresbury.ac.uk U.K. JANET MAKING SURE THAT YOUR INFORMATION IS CURRENT This notice will be mailed bimonthly to each newsgroup. You should check our WAIS source or waismail e-mail server from time-to-time to see if your address information is still up-to-date. Send the message help to waismail@net.bio.net for instructions on using waismail. Leave the Subject: line in your message blank. Using Gopher to complete the form --------------------------------- If you don't want to use a text editor, you can also use Dan Jacobson's gopher site to fill out the address database form as follows. Otherwise skip this section on gopher and proceed to the instructions for filling out the form below. > To add yourself to the database just point your > gopher client at merlot.gdb.org and select the following: > > --> 15. Searching For Biologists/ > > --> 9. E-mail Addresses of Biosci-Bionet Users/ > > --> 1. Add (or Correct) Your Address to the BIOSCI User Address > Data.. > > > And fill out the form. or Rob Harper's gopher site in Europe as follows: > Europeans can point their gopher client at gopher.csc.fi and add their > information to the database. All entries will be mailed directly to > Dave for incorporation in a wais source. > > The path to the questionare is as follows. > > ---> 10. Finnish EMBnet BioBox/ > > ---> 8. FAQ Files/ > > FAQ Files > > 1. EMBnet: Information. > 2. EMBnet: Internet resources guide. > 3. A Biologist's Guide to Internet Resources/ > 4. All FAQs (Frequently Asked Questions) Searches and Archives/ > --->5. Bionauts Address Database (questionaire) IMPORTANT INSTRUCTIONS - PLEASE READ CAREFULLY Please enter all responses after the : on each line, leaving one (1) blank space after the : (i.e., before the start of your text). Please do NOT extend your responses past the end of each line (80 characters) or alter any of the field identifiers such as "first name: ". Several lines are provided at the end of the form for comments, but, please adhere to the line length restriction. On the date: line, please enter the date in the DD-MM-YY format, e.g., 05-05-93 for 5 May 1993. This line will tell others when the information was last updated. Please be sure to include the 0's for single digit days or months, e.g., 05-05-93, not 5-5-93. Note that the "e-mail network: " line below is for specifying, e.g., "Internet," "BITNET," "EARN," "JANET," or whatever other network that your computer may be on. If you are uncertain about any field, please feel free to leave it blank, but please DO NOT DELETE the field identifier from the form! In the first field below, "New information or Update ...", please enter "N" if this is the first time that you have registered in the directory or "U" if you are correcting a listing that you sent to us previously. The comment: lines may be used for anything that you like but PLEASE DO NOT DELETE THEM FROM THE FORM OR ALTER THEM. One suggested use is to list the names of the newsgroups in which you participate. Please use the MAILING LIST name (see below - the latest version of the list can be requested from biosci@net.bio.net) instead of the USENET name even if you don't participate by e-mail. WAIS might get confused by the periods in the USENET names. This allows one to retrieve via WAIS or waismail the list of participants in a particular group. For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIO-SOFTWARE bionet.software CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMPLOYMENT bionet.jobs GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MOLECULAR-EVOLUTION bionet.molbio.evolution NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources TROPICAL-BIOLOGY bionet.biology.tropical VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-chromosomes@net.bio.net Tue Dec 14 22:00:00 1993 Path: biosci!GALAXY.GOV.BC.CA!PMACLEOD From: PMACLEOD@GALAXY.GOV.BC.CA (Patrick Macleod 727-4461) Newsgroups: bionet.genome.chromosomes Subject: Human chromosome 14 del(q32.1) Date: 14 Dec 1993 17:38:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: bionet Message-ID: <01H6H5X4308AB3X84S@gems.gov.bc.ca> NNTP-Posting-Host: net.bio.net I wish to contact anyone who has a case(s) of this condition and who is willing to share some of their experience with the natural history with the parents of our local case. Thank you PMacleod@galaxy.gov.bc.ca From owner-chromosomes@net.bio.net Wed Dec 15 22:00:00 1993 Newsgroups: bionet.genome.chromosomes Subject: Manic Depression Gene From: pete.dixon@canrem.com (Pete Dixon) Path: biosci!bcm!cs.utexas.edu!uunet!news2.uunet.ca!uunet.ca!uunet.ca!portnoy!canrem.com!pete.dixon Distribution: world Message-ID: <60.617.5254.0N18E77F@canrem.com> Date: Thu, 16 Dec 93 00:27:00 -0400 Organization: CRS Online (Toronto, Ontario) Lines: 19 At a recent meeting of my Manic Depressive group a video was shown where they talked about how a causal gene/gene set had been identified on chromosome 11. The identification was confirmed by testing affected and non-affected family members among the Amish and other gene mapping-friendly groups. The strong implication to me was that there was now a genetic marker test for this gene. If there is can someone direct to sources, costs, availability information about these tests. Even if the cost was in the 100's of dollars it would be cheap in relation to the damage last/mis-diagnosis can cause. FYI, most manics report 11 years and a series of mis-diagnoses before a Manic Depression conclusion was reached. That's a lot of talk therapy to sit through to find out one's problem is biochemical in origin. Not to mention the effect of those un-needed psychotropic drugs. Pete Dixon Kitchener, Ontario Canada From owner-chromosomes@net.bio.net Wed Dec 15 22:00:00 1993 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!uunet!pipex!uknet!doc.ic.ac.uk!mrccrc!crc.ac.uk!dcurtis From: dcurtis@crc.ac.uk (Dr. David Curtis) Subject: Re: Manic Depression Gene Message-ID: <1993Dec16.164403.11331@crc.ac.uk> Sender: news@crc.ac.uk Nntp-Posting-Host: tin Reply-To: dcurtis@crc.ac.uk (Dr. David Curtis) Organization: MRC Human Genome Mapping Project Resource Centre, Harrow, UK References: <60.617.5254.0N18E77F@canrem.com> Date: Thu, 16 Dec 1993 16:44:03 GMT Lines: 29 In article <60.617.5254.0N18E77F@canrem.com>, pete.dixon@canrem.com (Pete Dixon) writes: > At a recent meeting of my Manic Depressive group a video was shown where they > talked about how a causal gene/gene set had been identified on chromosome 11. > The identification was confirmed by testing affected and non-affected family > members among the Amish and other gene mapping-friendly groups. > > The strong implication to me was that there was now a genetic marker test for > this gene. If there is can someone direct to sources, costs, availability > information about these tests. We're _very_ far from such a situation. The Amish result may well have been a false positive. There are a few positive association studies suggesting a role for mutations in this region leading to manic depression, but just as many negative ones. Certainly many cases of manic depression are _not_ caused by genes in this region, so the results of any negative test could be difficult to interpret, certainly without testing many other members of a family. On the other hand, at any time there could be a big breakthrough which could bring about the possibilities you envisage. We've seen this happen recently with Alzheimer's disease (though not to the extent of having simple tests for the majority of cases) and there's no reason why manic depression shouldn't be next - we're all working hard enough at it. It just hasn't happened yet, that's all. Dave Curtis Academic Department of Psychiatry, Janet: dcurtis@UK.AC.CRC St. Mary's Hospital, Elsewhere: dcurtis@CRC.AC.UK Praed Street, London W2. EARN/Bitnet: dcurtis%CRC@UKACRL Tel 071-725 1993 Usenet: ...!mcsun!ukc!mrccrc!D.Curtis From owner-chromosomes@net.bio.net Sun Dec 19 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!paladin.american.edu!constellation!news.uoknor.edu!aardvark.ucs.uoknor.edu!broe From: broe@aardvark.ucs.uoknor.edu (Bruce Roe) Newsgroups: bionet.genome.chromosomes Subject: CEPH-Genethon Map Date: 19 Dec 1993 08:23 CST Organization: University of Oklahoma - University Computing Services Lines: 39 Distribution: world Message-ID: <19DEC199308234358@aardvark.ucs.uoknor.edu> NNTP-Posting-Host: 129.15.10.11 Keywords: chromosomes, map, ceph, genethon News-Software: VAX/VMS VNEWS 1.41 From what I gather, second/third had, Cohen's group had YAC contig maps completed for all the human chromosomes. This is very exciting news but only the first-generation map. Much more work is needed to get from here to a sequence ready map. Hats off to Cohen and co-workers for providing us all with this very useful and much needed data. What a great present to be given for this holiday season. On to the next stages of finer mapping and large scale sequencing!! The following was posted to bionet.general and I'm including it here for those who missed it: > The data of the ceph-genethon-map (Nature paper : > "A first-generation physical map of the human genome" > D. Cohen, I. Chumakov & J. Weissenbach ) > can be accessed by : > > ftp anonymous : ceph-genethon-map.genethon.fr > dir pub/ceph-genethon-map > > mail to : ceph-genethon-map@genethon.fr > > gopher : gopher.genethon.fr > -> Genethon Data > -> Pmap > > WWW : http://www.genethon.fr/genethon_en.html > > ------------------------------------------------------------- > Patricia RODRIGUEZ-TOME Genethon > Internet : pat@genethon.fr > ------------------------------------------------------------- Cheers.......bruce - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - \ Bruce A. Roe Professor of Chemistry and Biochemistry / / University of Oklahoma INTERNET: BROE@aardvark.ucs.uoknor.edu \ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - From owner-chromosomes@net.bio.net Wed Dec 22 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!nctuccca.edu.tw!TWNMOE10.Edu.TW!YMMCS010 From: YMMCS010@TWNMOE10.Edu.TW Newsgroups: bionet.genome.chromosomes Subject: Fluorescent in situ hybridization Date: Thu, 23 Dec 93 17:48:04 GMT Organization: MOE Computer Center, Taiwan Lines: 8 Message-ID: <16CADFA54.YMMCS010@TWNMOE10.Edu.TW> NNTP-Posting-Host: twnmoe10.edu.tw Keywords: FISH X-Newsreader: NNR/VM S_1.3.2 Hello, could someone provide me the information whether genomic DNA with its ve ctor, the lambda phage, could be used as the probe for FISH in genomic mapping? Because I have tried for several times but all failed. Please e-mail me to YMMCS010@TWNMOE10.EDU.TW. Thanks. Frank From owner-chromosomes@net.bio.net Thu Dec 23 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!nctuccca.edu.tw!TWNMOE10.Edu.TW!YMMCS023 From: YMMCS023@TWNMOE10.Edu.TW Newsgroups: bionet.genome.chromosomes Subject: CAG trinucleotide repeat Date: Sat, 25 Dec 93 04:05:02 GMT Organization: MOE Computer Center, Taiwan Lines: 9 Message-ID: <16CAF396E.YMMCS023@TWNMOE10.Edu.TW> NNTP-Posting-Host: twnmoe10.edu.tw X-Newsreader: NNR/VM S_1.3.2 Are there any groups interested in (or working on) cDNA with trinucleotide repe at? I am a clinical psychiatrist and interested in the genetics of psychiatric disorders, especially schizophrenia, bipolar disorder and Alzheimer's disease. I guess there is a chance inheritable psychiatric diseases are caused by trinuc leotide repeat expansion. Will I be right? What's the rationale to verify this idea? Anybody offering relevant information will be highly appreciated. Horng e-mail: ymmcs023@twnmoe10.edu.tw From owner-chromosomes@net.bio.net Thu Dec 23 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!pipex!doc.ic.ac.uk!daresbury!bioftp.unibas.ch!embl-heidelberg.de!rice From: rice@embl-heidelberg.de (Peter Rice) Newsgroups: bionet.genome.chromosomes Subject: Re: CAG trinucleotide repeat Message-ID: <1993Dec24.225853.146730@embl-heidelberg.de> Date: 24 Dec 93 22:58:53 +0100 References: <16CAF396E.YMMCS023@TWNMOE10.Edu.TW> Organization: EMBL, European Molecular Biology Laboratory Lines: 17 In article <16CAF396E.YMMCS023@TWNMOE10.Edu.TW>, YMMCS023@TWNMOE10.Edu.TW writes: > I guess there is a chance inheritable psychiatric diseases are caused by trinuc > leotide repeat expansion. Will I be right? If Huntingdon's Chorea or Fragile X Syndrome count as psychiatric disorders, then you are right already. (Or perhaps it takes a dinucleotide repeat to make someone truly GAGA :-) ----------------------------------------------------------------------------- Peter Rice, EMBL | Post: Computer Group | European Molecular Internet: Peter.Rice@EMBL-Heidelberg.DE | Biology Laboratory | Postfach 10-2209 Phone: +49-6221-387247 | 69012 Heidelberg Fax: +49-6221-387306 | Germany From owner-chromosomes@net.bio.net Sun Dec 26 22:00:00 1993 Path: biosci!rutgers!cs.utexas.edu!howland.reston.ans.net!nctuccca.edu.tw!TWNMOE10.Edu.TW!YMMCS023 From: YMMCS023@TWNMOE10.Edu.TW Newsgroups: bionet.genome.chromosomes Subject: How to compare a strech of sequence with genebank Message-ID: <16CB21457.YMMCS023@TWNMOE10.Edu.TW> Date: 28 Dec 93 01:26:47 GMT Organization: MOE Computer Center, Taiwan Lines: 11 NNTP-Posting-Host: twnmoe10.edu.tw X-Newsreader: NNR/VM S_1.3.2 I got some sequences from human fetal brain cDNA. Where could I compare these uences with DNA bank (requiring no specific password) on internet to see if I h ave got something new or something old. Any information will be highly appresci ated. Sincerely CHEN-JEE HORNG, M.D. DEPARTMENT OF PSYCHIATRY VETERANS GENERAL HOSPITAL-TAIPEI TAIPEI, TAIWAN, R.O.C. From owner-chromosomes@net.bio.net Mon Dec 27 22:00:00 1993 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!nctuccca.edu.tw!TWNMOE10.Edu.TW!YMMCS023 From: YMMCS023@TWNMOE10.Edu.TW Newsgroups: bionet.genome.chromosomes Subject: Comparing DNA sequence in foreign host Message-ID: <16CB211153.YMMCS023@TWNMOE10.Edu.TW> Date: 28 Dec 93 19:26:11 GMT Organization: MOE Computer Center, Taiwan Lines: 61 NNTP-Posting-Host: twnmoe10.edu.tw X-Newsreader: NNR/VM S_1.3.2 I know some kind persone has mailed me rich information. I am sorry I lost it b efore reading it, and I don't remember the name and address of the kind person. I got some sequences from human fetal brain cDNA. Where could I compare these uences with DNA bank (requiring no specific password) on internet to see if I h ave got something new or something old. Any information will be highly appresci ated. Sincerely CHEN-JEE HORNG, M.D. DEPARTMENT OF PSYCHIATRY VETERANS GENERAL HOSPITAL-TAIPEI TAIPEI, TAIWAN, R.O.C. ======================================================================== From: YMMCS023@TWNMOE10.Edu.TW Path: TWNMOE10.Edu.TW!YMMCS023 Newsgroups: bionet.molbio.bio-matrix Subject: compare DNA sequence on internet Message-ID: <16CB21B51.YMMCS023@TWNMOE10.Edu.TW> Date: Tue, 28 Dec 93 01:56:33 GMT Organization: MOE Computer Center, Taiwan X-Newsreader: NNR/VM S_1.3.2 I got some sequences from human fetal brain cDNA. Where could I compare these uences with DNA bank (requiring no specific password) on internet to see if I h ave got something new or something old. Any information will be highly appresci ated. Sincerely CHEN-JEE HORNG, M.D. DEPARTMENT OF PSYCHIATRY VETERANS GENERAL HOSPITAL-TAIPEI TAIPEI, TAIWAN, R.O.C. ======================================================================== From: YMMCS023@TWNMOE10.Edu.TW Path: TWNMOE10.Edu.TW!YMMCS023 Newsgroups: bionet.molbio.gdb Subject: compare DNA sequence on anonymous DNA bank host Message-ID: <16CB21D4A.YMMCS023@TWNMOE10.Edu.TW> Date: Tue, 28 Dec 93 02:04:58 GMT Organization: MOE Computer Center, Taiwan X-Newsreader: NNR/VM S_1.3.2 I got some sequences from human fetal brain cDNA. Where could I compare these uences with DNA bank (requiring no specific password) on internet to see if I h ave got something new or something old. Any information will be highly appresci ated. Sincerely CHEN-JEE HORNG, M.D. DEPARTMENT OF PSYCHIATRY VETERANS GENERAL HOSPITAL-TAIPEI TAIPEI, TAIWAN, R.O.C.