From owner-chromosomes@net.bio.net Wed May 11 23:00:00 1994
Path: biosci!MERCURY.UARK.EDU!DRHOADS
From: DRHOADS@MERCURY.UARK.EDU ("Doug Rhoads")
Newsgroups: bionet.genome.chromosomes
Subject: Looking for a BAC vector
Date: 12 May 1994 07:05:59 -0700
Organization: University of Arkansas
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Sender: daemon@net.bio.net
Distribution: bionet
Message-ID: <11783F42855@uamercury.uark.edu>
NNTP-Posting-Host: net.bio.net

We would like to construct a Bacterial Artificial Chromosome library.  We 
have been unable to contact the authors of the PNAS 89:8794-8797 paper.  
Anyone out there know a convenient source??

Doug Rhoads                  || Dept. of Biological Sciences
drhoads@mercury.uark.edu     || 601 Science Engineering
drhoads@uafsysb.uark.edu     || University of Arkansas
501-575-3251                 || Fayetteville, AR 72701

From owner-chromosomes@net.bio.net Thu May 12 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail
From: URA1335@ARTHUR.CITI2.FR
Newsgroups: bionet.genome.chromosomes
Subject: HELP: NEED BREAST CANCER cDNA LIBRARY
Date: 13 May 1994 14:52:09 +0100
Lines: 17
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2r00m9$54g@mserv1.dl.ac.uk>
Original-To: biochrom@dl.ac.uk

Dear netters,

we need this breast cancer cDNA library

if possible from hormone-responsive cancer cell

lines, i.e. MCF7 cell line, in order to isolate

tissue-specific cDNAs by hybridization techniques.

Any help is taken into the account of collaborative

research. Thank you from advance

Dr ARAL

E-Mail: URA1335@citi2.FR

From owner-chromosomes@net.bio.net Wed May 18 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!gatech!news-feed-1.peachnet.edu!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!dsinc!netnews.upenn.edu!pythia.humgen.upenn.edu!eckman
From: eckman@pythia.humgen.upenn.edu (Barbara Eckman)
Newsgroups: bionet.genome.chromosomes
Subject: Chromosome 22 anonymous ftp site
Date: 19 May 1994 15:18:23 GMT
Organization: University of Pennsylvania
Lines: 36
Distribution: world
Message-ID: <2rfvvv$9j7@netnews.upenn.edu>
NNTP-Posting-Host: pythia.humgen.upenn.edu


The Philadelphia Center for Chromosome 22, located at the Children's 
Hospital of Philadelphia, Fox Chase Cancer Center, and the University of
Pennsylvania School of Medicine, is pleased to announce its new
anonymous ftp site.  The following reports are currently available: 

STS.txt		ASCII text file containing a report of over 300 STS's
		on Chromosome 22:  name, lab, locus, primer sequence,
		PCR product size, polymorphism, annealing temperature,
		and regional sublocalization. 

YAChits.txt	ASCII text file containing summary of YACs
		identified by probes, with subtotals by library.

To access:

   % ftp cbil.humgen.upenn.edu 
   -- log in as <anonymous>--
   ftp> cd pub/22

We hope the research community will find this data useful.

-------------------------------------------------------------------------
Barbara Eckman 
Programmer/Analyst, Database Administrator
Computational Biology and Informatics Laboratory
University of Pennsylvania 

eckman@cbil.humgen.upenn.edu
(215) 573-3116                Fax (215) 573-3111
-------------------------------------------------------------------------






From owner-chromosomes@net.bio.net Wed May 18 23:00:00 1994
Path: biosci!daresbury!not-for-mail
From: aschafer@crc.ac.uk (Dr. A.J. Schafer)
Newsgroups: bionet.genome.chromosomes
Subject: Yeast chromosome sizes, restriction mapping programs.
Date: 19 May 1994 18:11:03 +0100
Lines: 50
Sender: daemon@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <2rg6j7$j5d@mserv1.dl.ac.uk>
Original-To: biochrom@dl.ac.uk

I am
mapping a
big YAC,
about 2Mb,
and could
use help
finding
out
the
following:
1. Does
anyone
know the
yeast
strain
that the
CEPH YACs
are in ,
and
especially, their size.
2. Are
there any
available
good
(preferably Macintosh) computer programs
for (1)
determining the size of a restriction fragment from mobility
of size
standards
and
mobility
of
unknown,
and(2) for
generating
restriction
maps from
digests
and
fragment
sizes.
Thanks to
all in
advance,
Alan
-----------------------------------------------------------------------------
Alan Schafer, Ph.D.                   e-mail: aschafer@hgmp.mrc.ac.uk
University of Cambridge               
Department of Genetics                
-----------------------------------------------------------------------------

From owner-chromosomes@net.bio.net Thu May 19 23:00:00 1994
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!news1.digex.net!usenet
From: sdoyle@cadillac.digex.net
Newsgroups: bionet.genome.chromosomes
Subject: Prader_willi Genome information
Date: 20 May 1994 16:08:01 GMT
Organization: Citizens United
Lines: 9
Distribution: world
Message-ID: <2rin91$ms5@news1.digex.net>
Reply-To: sdoyle@cadillac.digex.net
NNTP-Posting-Host: cadillac.digex.net
X-Newsreader: IBM NewsReader/2 v1.00


Could someone point me in the right direction for genetic info on Prader-Willi
syndrome.

How about chromosonal defects.

Sean Doyle
sdoyle@cadillac.digex.net


From owner-chromosomes@net.bio.net Thu May 19 23:00:00 1994
Path: biosci!BIOTECHNET.COM!NISSON
From: NISSON@BIOTECHNET.COM
Newsgroups: bionet.genome.chromosomes
Subject: unstabile cDNAs?
Date: 20 May 1994 10:03:31 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <01HCKA5UAGVK95MXZS@delphi.com>
NNTP-Posting-Host: net.bio.net

Does anyone have any cDNA clones that contain unstabile sequences within
them such that after their propagation, parts of the clone are lost? I'm 
interested in characterizing a novel E. coli strain for its ability to 
stably maintain cDNA clones that has been shown to be unstabile in other
hosts.

From owner-chromosomes@net.bio.net Thu May 19 23:00:00 1994
Path: biosci!bcm!mbcr.bcm.tmc.edu!jamess
From: jamess@mbcr.bcm.tmc.edu (James S. Sutcliffe)
Newsgroups: bionet.genome.chromosomes
Subject: Re: Prader_willi Genome information
Date: 20 May 1994 18:52:44 GMT
Organization: Baylor College of Medicine, Houston, Tx
Lines: 42
Distribution: world
Message-ID: <2rj0ts$i25@gazette.bcm.tmc.edu>
References: <2rin91$ms5@news1.digex.net>
NNTP-Posting-Host: mbcr.bcm.tmc.edu

In article <2rin91$ms5@news1.digex.net>, sdoyle@cadillac.digex.net writes:
|> 
|> Could someone point me in the right direction for genetic info on Prader-Willi
|> syndrome.
|> 
|> How about chromosonal defects.
|> 
|> Sean Doyle
|> sdoyle@cadillac.digex.net
|> 

Sean,

Prader-Willi syndrome is caused by genetic deficiency in chromosomal region
15q11-q13. The deficiency can result from a deletion of the
paternally-derived chromosome or uniparental disomy for the maternally-derived
chromosome (2 copies of maternal 15).

Several groups (including ours) are doing molecular genetic studies on this
region to identify genes which may be involved in this disorder. There is
currently one published candidate gene: the gene encoding the small nuclear
ribonucleoprotein-associated polypeptide N (SNRPN). 

While deficiency of a paternal 15q11-q13 results in Prader-Willi syndrome,
deficiency in the maternal chromosome for the same region gives rise to
another disorder termed Angelman syndrome. This indicates that PWS is caused
by a loss of paternally-expressed genes and AS is caused by a loss of
maternally expressed gene(s).

Please e-mail me if you would like additional information. Hope this helps.

**********************************************
* James S. Sutcliffe, Ph.D.                  *
* Howard Hughes Medical Institute            *
* Department of Molecular and Human Genetics *
* Baylor College of Medicine                 *
* Houston, TX 77030                          *
*                                            *
* Tel: 713-798-4396/4795                     * 
* Fax: 713-797-6718                          *
* internet: jamess@bcm.tmc.edu               *
**********************************************

From owner-chromosomes@net.bio.net Thu May 19 23:00:00 1994
Newsgroups: bionet.genome.chromosomes
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!howland.reston.ans.net!wupost!monsanto.com!rcwieg.monsanto.com!user
From: rcwieg@ccmail.monsanto.com (Roger Wiegand)
Subject: Re: Prader_willi Genome information
Message-ID: <rcwieg-200594131933@rcwieg.monsanto.com>
Followup-To: bionet.genome.chromosomes
Sender: news@tin.monsanto.com (USENET News System)
Organization: Searle Molecular Biology
References: <2rin91$ms5@news1.digex.net>
Date: Fri, 20 May 1994 18:19:33 GMT
Lines: 38

In article <2rin91$ms5@news1.digex.net>, sdoyle@cadillac.digex.net wrote:

> 
> Could someone point me in the right direction for genetic info on Prader-Willi
> syndrome.
> 
> How about chromosonal defects.
> 
> Sean Doyle
> sdoyle@cadillac.digex.net

Taken from *extensive* information in the Online Mendelian Inheritance in
Man, available by pointing your gopher to the Computational Biology
(Welchlab-Johns Hopkins) server for biological databases:


Occurrence of the Prad.
er-Willi Syndrome

The vast majority of PWS cases occur sporadically.  These instances
include virtually all interstitial deletions, the large majority of
de novo unbalanced translocations, all instances of maternal
uniparental disomy with normal karyotype with a de novo rearrangement
involving chromosome 15 and almost all cases of maternal uniparental
disomy with a familial rearrangement involving chromosome 15.  There
is no parental age effect whatsoever in the deletion cases.

For full discussion on the mode of inheritance, see Cytogenetics,
below.



-- 
Thanks,
Roger

rcwieg@ccmail.monsanto.com
"If you push it hard enough, it *will* fall over"

From owner-chromosomes@net.bio.net Fri May 20 23:00:00 1994
Path: biosci!biosci!not-for-mail
From: dadler@u.washington.edu (David A. Adler)
Newsgroups: bionet.announce,bionet.genome.chromosomes,bionet.molbio.methds-reagnts
Subject: UW Pathology Web Server
Date: 20 May 1994 19:13:49 -0700
Organization: University of Washington
Lines: 15
Sender: kristoff@net.bio.net
Approved: bionews-moderator@net.bio.net
Distribution: bionet
Message-ID: <2rintu$18j@news.u.washington.edu>
Reply-To: dadler@u.washington.edu
NNTP-Posting-Host: net.bio.net
Xref: biosci bionet.announce:1144 bionet.genome.chromosomes:230 bionet.molbio.methds-reagnts:14496

The University of Washington Pathology's Web Server is now up and running.  
The server includes access to human and mouse chromosome idiograms and  
photomicrograph scans; the Genome Machine: a simple graphical interface  
experiment; a pathology gallery (under construction); and information  
about the UW Medical Scientists Training Program (MSTP).

The URL for this site is:
	http://www.pathology.washington.edu

--
David A. Adler                  Pathology SM-30
University of Washington        Seattle, WA 98195
(206) 543-0716 (phone)		(206) 543-3644 (fax)
"Science is nothing but trained and organized common sense"
T.H.Huxley

From owner-chromosomes@net.bio.net Fri May 20 23:00:00 1994
Newsgroups: bionet.genome.chromosomes
Path: biosci!agate!howland.reston.ans.net!gatech!swrinde!emory!cs.utk.edu!martha.utcc.utk.edu!bme!gaoli
From: gaoli@bme.utmem.edu (gaoli)
Subject: cDNA probes for stromelysin and collagenase from bovine tissue.
Message-ID: <1994May21.035306.2999@martha.utcc.utk.edu>
Keywords: cDNA probes for stromelysin and collagenase.
Lines: 11
Sender: gaoli@bme (gaoli)
Reply-To: gaoli@bme.utmem.edu (gaoli)
Organization: University of Tennessee, Memphis
Date: Sat, 21 May 1994 03:53:06 GMT


I am looking for cDNA probes for stromelysin and collagenase from bovine
tissue. Any help would be deeply appreciated. Please E-mail me at below
address:

gaoli@bme.utmem.edu
gaoli@eeg1.lcmc.edu

Thanks in advance.

gaoli

From owner-chromosomes@net.bio.net Fri May 20 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!convex!convex!constellation!news.uoknor.edu!ns1.nodak.edu!netnews.nwnet.net!news.u.washington.edu!news
From: dadler@u.washington.edu (David Adler)
Newsgroups: bionet.genome.chromosomes
Subject: UW Pathology Web Server
Date: 21 May 1994 04:15:21 GMT
Organization: University of Washington
Lines: 14
Message-ID: <2rk1sp$hoj@news.u.washington.edu>
Reply-To: dadler@u.washington.edu
NNTP-Posting-Host: koko.pathology.washington.edu

We are pleased to announce the availability of a Web server from the  
Department of Pathology, University of Washington. Included at this site  
are human and mouse chromosome idiograms and photomicrograph scans; a  
Genome Machine: simple graphical interface experiment; a pathology gallery  
(under construction); and information about the UW Medical Scientists  
Training Program (MSTP).
thanks,
David
--
David A. Adler                  Pathology SM-30
University of Washington        Seattle, WA 98195
(206) 543-0716 (phone)		(206) 543-3644 (fax)
"Science is nothing but trained and organized common sense"
T.H.Huxley

From owner-chromosomes@net.bio.net Fri May 20 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!news
From: dadler@u.washington.edu (David Adler)
Newsgroups: bionet.genome.chromosomes
Subject: UW Pathology Web Server, Revisited
Date: 21 May 1994 04:19:35 GMT
Organization: University of Washington
Lines: 13
Message-ID: <2rk24n$hp0@news.u.washington.edu>
Reply-To: dadler@u.washington.edu
NNTP-Posting-Host: koko.pathology.washington.edu

Oops!
The URL for the UW Pathology Web Server is:

	http://www.pathology.washington.edu

sorry for the omission and extra post,
David
--
David A. Adler                  Pathology SM-30
University of Washington        Seattle, WA 98195
(206) 543-0716 (phone)		(206) 543-3644 (fax)
"Science is nothing but trained and organized common sense"
T.H.Huxley

From owner-chromosomes@net.bio.net Mon May 23 23:00:00 1994
Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!newsxfer.itd.umich.edu!zip.eecs.umich.edu!umn.edu!mayonews.mayo.edu!Robert.Jenkins,.M.D.,.Ph.D.
From: ROBERT_JENKINS@MSGW.MAYO.EDU (Robert Jenkins, M.D., Ph.D)
Newsgroups: bionet.genome.chromosomes
Subject: test, Don't read
Date: 24 May 1994 16:08:50 GMT
Organization: Mayo Foundation
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NNTP-Posting-Host: jenkinsr.mayo.edu
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Xdisclaimer: No attempt was made to authenticate the sender's name.



just a test

From owner-chromosomes@net.bio.net Tue May 24 23:00:00 1994
Path: biosci!daresbury!trane.uninett.no!sunic!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!news.ans.net!newstf01.cr1.aol.com!search01.news.aol.com!not-for-mail
From: ellie43@aol.com (ELLIE43)
Newsgroups: bionet.genome.chromosomes
Subject: Fragile x syndrome
Date: 25 May 1994 12:16:03 -0400
Organization: America Online, Inc. (1-800-827-6364)
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Sender: news@search01.news.aol.com
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NNTP-Posting-Host: search01.news.aol.com

A friends son was just diagnosed with this problem.  I would like to
find any information  that is available about this.                  
                                              

                                 Thanks

From owner-chromosomes@net.bio.net Tue May 24 23:00:00 1994
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!howland.reston.ans.net!gatech!nntp.msstate.edu!saimiri.primate.wisc.edu!news.doit.wisc.edu!f181-196.net.wisc.edu!user
From: Kckim@students.wisc.edu (Konrad C. Kim)
Newsgroups: bionet.genome.chromosomes
Subject: biotech job
Followup-To: bionet.genome.chromosomes
Date: 25 May 1994 22:04:33 GMT
Organization: Univeristy of Wisconsin
Lines: 52
Distribution: world
Message-ID: <Kckim-250594180418@f181-196.net.wisc.edu>
NNTP-Posting-Host: f181-196.net.wisc.edu

KONRAD C. KIM
	16 White Deer Ct.
	Huntington, NY 11743
	(516)549-5205

EDUCATION:
	University of Wisconsin-Madison:
		College of Letters and Sciences,  B.S  Zoology 1988-92Õ
		College of Agriculture and Life Sciences, B.S.  Genetics 1993-94Õ

	Related Courses taken:
		Biochemistry, Cell Biology, Organic Chemistry, Endocrinology, Cell
Physiology,
		Neurobiology, 	Microbiology, Soil Microbiology and Biochemistry, Human
Genetics

WORK EXPERIENCE:

	Junior Lab Technician,     6/92-6/93
	University of Minnesota Hospital and Clinics
		Department of Therapeutic Radiology Radiation Oncology
		Radiation Biology Section
	Supervisor: Beth Auger. Assistant Professor Ph.D.
	Principal Investigator: Changwon Song. Professor Ph.D.
		424 Harvard St. SE												
    Minneapolis, MN 55455
		612-626-6090

	Biological Assistant,    1/92-6/92
	Veterans Memorial Middelton Hospital                                      

		Department of Neurology
	Supervisor: Jim Vann. Senior Lab Scientist and Manager
		1134 Terrace Av.												
     Madison WI 53715

SKILLS:

	Protein Isolation: 
		SDS-PAGE, Affinity chromotography, Dialysis/Salting out, Agglutination 	 
 	
	Tissue Culture: 
		Mark I Cesium Irradiation Chamber, Fsa2 and SCK Cell Types
	Genetics: 
		Southern, Western, and Northern Blots, Gel Electrophoresis, PCR

AWARDS AND HOBBIES:

	Eagle Scout
	University of Wisconsin All-String Orchestra 1988-92Õ

References on request

From owner-chromosomes@net.bio.net Sun May 29 23:00:00 1994
Path: biosci!agate!howland.reston.ans.net!wupost!waikato!comp.vuw.ac.nz!canterbury.ac.nz!chmeds.ac.nz!mkennedy
From: mkennedy@chmeds.ac.nz (Martin Kennedy)
Newsgroups: bionet.genome.chromosomes
Subject: Re: Fragile x syndrome
Message-ID: <1994May30.151426.510@chmeds.ac.nz>
Date: 30 May 94 15:14:26 +1200
References: <2rvtk3$n24@search01.news.aol.com>
Lines: 23

In article <2rvtk3$n24@search01.news.aol.com>, ellie43@aol.com (ELLIE43) writes:
...Fragile X syndrome...
> A friends son was just diagnosed with this problem.  I would like to
> find any information  that is available about this.                  

This is a very active area, and there is a lot of literature.  Some general 
refs are:

J Med Genet 28, 811-813
Trends in Genetics 8, 249-255

If you want more specialized refs or info, email me; we do the DNA test for 
this disorder.

Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy@chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
	      Phone (64-3)364-0880  Fax (64-3)364-0750

From owner-chromosomes@net.bio.net Tue May 31 23:00:00 1994
Newsgroups: bionet.genome.chromosomes
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newsflash.concordia.ca!CC.UMontreal.CA!cumin.telecom.uqam.ca!news
From: Barron, Claude <barron.claude@uqam.ca>
Subject: chromosome 1
Message-ID: <FZAPBLCD@cumin.telecom.uqam.ca>
X-Xxdate: Wed, 1 Jun 94 13:21:50 GMT
Sender: news@cumin.telecom.uqam.ca
Nntp-Posting-Host: 132.208.163.160
Organization: UQAM
X-Useragent: Version 1.1.3
Date: Wed, 1 Jun 1994 18:14:03 GMT
Lines: 6

One of my friends had a baby 5 monts ago with a defect on chromosome 1. 
Does anyone know anything about this number one?

thanks,
Claude Barron
email barron.claude@uqam.ca

From owner-chromosomes@net.bio.net Tue May 31 23:00:00 1994
Newsgroups: bionet.genome.chromosomes
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newsflash.concordia.ca!CC.UMontreal.CA!cumin.telecom.uqam.ca!news
From: Barron, Claude <barron.claude@uqam.ca>
Subject: chromosome 1
Message-ID: <SZAPBY2D@cumin.telecom.uqam.ca>
X-Xxdate: Wed, 1 Jun 94 18:34:53 GMT
Sender: news@cumin.telecom.uqam.ca
Nntp-Posting-Host: 132.208.163.160
Organization: UQAM
X-Useragent: Version 1.1.3
Date: Wed, 1 Jun 1994 18:27:06 GMT
Lines: 6

One of my friends had a baby 5 monts ago with a defect on chromosome 1. 
Does anyone know anything about this number one?

thanks,
Claude Barron
email barron.claude@uqam.ca

From owner-chromosomes@net.bio.net Tue May 31 23:00:00 1994
Newsgroups: bionet.genome.chromosomes
Path: biosci!daresbury!trane.uninett.no!eunet.no!nuug!EU.net!uunet!newsflash.concordia.ca!CC.UMontreal.CA!cumin.telecom.uqam.ca!news
From: Barron, Claude <barron.claude@uqam.ca>
Subject: chromosome 1
Message-ID: <HZAPBAHD@cumin.telecom.uqam.ca>
X-Xxdate: Wed, 1 Jun 94 13:23:39 GMT
Sender: news@cumin.telecom.uqam.ca
Nntp-Posting-Host: 132.208.163.160
Organization: UQAM
X-Useragent: Version 1.1.3
Date: Wed, 1 Jun 1994 18:15:54 GMT
Lines: 6

One of my friends had a baby 5 monts ago with a defect on chromosome 1. 
Does anyone know anything about this number one?

thanks,
Claude Barron
email barron.claude@uqam.ca

