From owner-chromosomes@net.bio.net Sat Oct 01 23:00:00 1994 Path: biosci!agate!library.ucla.edu!news.mic.ucla.edu!unixg.ubc.ca!nntp.cs.ubc.ca!torn!news.unb.ca!coranto.ucs.mun.ca!nstn.ns.ca!nstn.ns.ca!nntp-user From: awalsh@fox.nstn.ns.ca (Jay Walsh) Newsgroups: bionet.genome.chromosomes Subject: Genome Date: 2 Oct 1994 17:41:01 -0300 Organization: Nova Scotia Technology Network Lines: 8 Sender: news@nstn.ns.ca Message-ID: <36n5st$fcp@Owl.nstn.ca> NNTP-Posting-Host: owl.nstn.ns.ca X-Newsreader: WinVN version 0.82 A friend of mine is looking for some information on the human genome project. I thought this would be a good place to start. I was hoping someone could give me some info or at least direct me to the source. He was looking for the moral and scientific aspects as well as the implications. I anticipate your response. Jay Walsh From owner-chromosomes@net.bio.net Sun Oct 02 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!prairienet.org!dstone From: dstone@prairienet.org (David M. Stone) Newsgroups: bionet.genome.chromosomes Subject: Nontraditional Inheritance Date: 3 Oct 1994 22:58:28 GMT Organization: University of Illinois at Urbana Lines: 10 Message-ID: <36q2ak$k7r@vixen.cso.uiuc.edu> NNTP-Posting-Host: firefly.prairienet.org I recently received a grant for a teacher update workshop. I am interested in having a number of speakers present regarding a number of topics. Can anyone tell me specifically which topics fit under the "umbrella" of nontraditional inheritance? -- David M. Stone (Teaching Associate) Home: 803 E. Olympian Rd. University High School Urbana, IL 61801 1212 W. Springfield Ave., Urbana, IL 61801 (217) 643-7622 (217) 333-2870 From owner-chromosomes@net.bio.net Mon Oct 03 23:00:00 1994 Path: biosci!bcm!NewsWatcher!user From: rossiter@bcm.tmc.edu (Belinda J.F. Rossiter) Newsgroups: bionet.genome.chromosomes Subject: Re: Nontraditional Inheritance Date: 4 Oct 1994 15:23:21 GMT Organization: Baylor College of Medicine Lines: 17 Message-ID: References: <36q2ak$k7r@vixen.cso.uiuc.edu> NNTP-Posting-Host: bg.imgen.bcm.tmc.edu In article <36q2ak$k7r@vixen.cso.uiuc.edu>, dstone@prairienet.org (David M. Stone) wrote: > I recently received a grant for a teacher update workshop. > I am interested in having a number of speakers present regarding > a number of topics. Can anyone tell me specifically which topics > fit under the "umbrella" of nontraditional inheritance? How about the phenomenon of trinucleotide repeat expansion leading to earlier onset and more severe disease as the gene is passed through the family? Examples of such diseases include myotonic dystrophy, Huntington disease, fragile X syndrome, spinocerebellar ataxia type 1, spinal and bulbar muscular atrophy, dentatorubral-pallidoluysian atrophy, and FRAXE mental retardation. Belinda Rossiter rossiter@bcm.tmc.edu From owner-chromosomes@net.bio.net Mon Oct 03 23:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!uunet!sangam!iitb!powai!shindeaw From: shindeaw@powai.cc.iitb.ernet.in (A. W. Shinde) Subject: CDNA FOR A.NIGER Message-ID: Date: Tue, 4 Oct 1994 15:33:33 GMT Organization: Computer Centre, Indian Institute of Technology, Bombay X-Newsreader: TIN [version 1.2 PL2] Lines: 1 From owner-chromosomes@net.bio.net Sun Oct 09 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!uunet!illuminati.io.com!nobody From: buc@pentagon.io.com (Craig Fox) Newsgroups: bionet.genome.chromosomes Subject: Laboratory Management Systems Date: 10 Oct 1994 14:49:17 -0500 Organization: Illuminati Online Lines: 11 Sender: buc@io.com Message-ID: <37c5rt$mof@pentagon.io.com> NNTP-Posting-Host: pentagon.io.com Does anyone out there have any experience with computer networks in the laboratory? At the cytogenetics lab where I work the MIS Dept. has installed a computer network to track specimens from the time that they are accessed to the results call-out. This has been very useful for our client services Dept. but it has been a real pain in the ass for our tissue culture staff. I was wondering if anyone has been through this process and can offer any any suggestions that might help. Thanx buc From owner-chromosomes@net.bio.net Sun Oct 09 23:00:00 1994 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 10 Oct 1994 02:00:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 322 Sender: daemon@net.bio.net Distribution: world Message-ID: <199410100900.CAA10710@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us a complete new updated form. We can not make manual revisions to existing entries. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net **** How to cancel a BIOSCI e-mail subscription **** If you want to cancel your e-mail subscription to this group, PLEASE DO NOT POST YOUR UNSUBSCRIBE REQUEST TO THE NEWSGROUP ADDRESS (NOR REPLY TO A MESSAGE POSTED TO THE NEWSGROUP)!!! This would send your request to all of the readers of the newsgroup, but it might still not be seen by the BIOSCI staff - thus you would annoy many people and possibly not accomplish your goal anyway. 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Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-chromosomes@net.bio.net Sun Oct 09 23:00:00 1994 Path: biosci!ARUBA.NYSAES.CORNELL.EDU!fsc From: fsc@ARUBA.NYSAES.CORNELL.EDU (Frank Cheng) Newsgroups: bionet.genome.chromosomes Subject: RDA Date: 9 Oct 1994 19:20:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Has anyone out there tried representational difference analysis (RDA) in order to find probes useful for genetic studies? Is that possible to isolate genes by cloning the differences between two genomes? Are there any references except the original paper (Science 259:946-951)? Thanks in advance Frank Cheng Dept. of Hort. Sci. Cornell University NY From owner-chromosomes@net.bio.net Sun Oct 09 23:00:00 1994 Path: biosci!biosci!not-for-mail From: sheaj@ohsu.EDU (Jackson Shea) Newsgroups: bionet.announce,bionet.genome.chromosomes,bionet.molbio.methds-reagnts Subject: ANNOUNCING: ALF DNA Sequencer Discussions in bionet.genome.chromosomes Followup-To: bionet.genome.chromosomes Date: 10 Oct 1994 14:37:20 -0700 Organization: Oregon Health Sciences University Lines: 39 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <37c4ph$rbg@steele.ohsu.edu> NNTP-Posting-Host: net.bio.net Keywords: ALF, DNA, Sequencing Xref: biosci bionet.announce:1480 bionet.genome.chromosomes:301 bionet.molbio.methds-reagnts:19453 DISCUSSION OF Pharmacia ALF DNA Sequencer NOW TAKING PLACE IN: bionet.genome.chromosomes The groundwork has been laid and I have "unofficially" polled the above-mentioned newsgroup for interest, and the response was encouraging. So, come one, come all. Whether you own an ALF, are considering an ALF, or own an ABI, please join in. Aside from just a general discussion of tips and hints for optimal results from the ALF sequencer (such as miniprep methods used on ALF), we'd like to see some discussions about the following: o What applications are being developed for using the ALF, (beyond simple DNA sequencing? [sizing and comparing dna fragments] o Special sequencing techniques/applications (homology analysis?). ...just to name a couple. In case you're wondering why this isn't taking place in bionet.molbio.methds- reagnts: One of the respondents to my informal poll asked this question and my responses were: * I initially contacted the bionet moderator, Dave Kristofferson and he suggested I use this bionet.genome.chromosomes because discussions about dna sequencing was in its charter (thanks to Bruce A. Roe from the Depart- ment of Chemistry and Biochemistry at the University of Oklahoma, one of the charter members). * Bionet.genome.chromosomes sees considerably less traffic than bionet.molbio.methds-reagnts. Please direct questions or comments to the newsgroup, or if you'd like you may email to: sheaj@ohsu.edu Jackson Shea, Systems Analyst, Oregon Health Sciences Univers. fishk@ohsu.edu Ken Fish, Core Facility Director, Oregon Health Sci. Univ. RichWalko@aol.com Rich Walko, Molec. Bio. Systems Group, Pharmacia From owner-chromosomes@net.bio.net Tue Oct 11 23:00:00 1994 Path: biosci!daresbury!bioftp.unibas.ch!rc1.vub.ac.be!is1e!bran From: bran@vub.ac.be (Andre B.) Newsgroups: bionet.genome.chromosomes Subject: Yes, do it ...help Date: 12 Oct 1994 21:57:37 GMT Organization: Brussels Free Universities (VUB/ULB), Belgium Lines: 9 Message-ID: <37hm4h$1v0@rc1.vub.ac.be> NNTP-Posting-Host: is1e.bfu.vub.ac.be. X-Newsreader: TIN [version 1.2 PL2] Were are implicated in sequencing yeast genome and we used the ALF system. Although the results are good, we encounter some problems. Might be useful to share impressions and solutions. Bruno ANDRE Univ. Brussels BELGIUM From owner-chromosomes@net.bio.net Thu Oct 13 23:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!news.dfn.de!news.belwue.de!news.uni-ulm.de!rz.uni-karlsruhe.de!stepsun.uni-kl.de!sun.rhrk.uni-kl.de!nenno From: nenno@rhrk.uni-kl.de (Mario Nenno [Biologie]) Subject: Re: Laboratory Management Systems Message-ID: <1994Oct14.203737.16624@rhrk.uni-kl.de> Organization: University of Kaiserslautern, Germany References: <37c5rt$mof@pentagon.io.com> Date: Fri, 14 Oct 1994 20:37:37 GMT Lines: 29 Hallo, buc@pentagon.io.com (Craig Fox) writes: >Does anyone out there have any experience with computer networks in the >laboratory? At the cytogenetics lab where I work the MIS Dept. has >installed a computer network to track specimens from the time that they >are accessed to the results call-out. This has been very useful for our >client services Dept. but it has been a real pain in the ass for our >tissue culture staff. I was wondering if anyone has been through this >process and can offer any any suggestions that might help. From the IGD Workshop I've heard a talk about a 'LabBase Query Language' (N. Goodman, S. Rozen, L. Stein, MIT, Cambridge/USA). They have a WWW-Server with informations, they said. The adress is: http://www-genome.wi.mit.edu I haven't tried it so far. Hope this helps. Mario _____________ / Mario Nenno \\ ___/ \\___________________________________________ | Universitaet Kaiserslautern 67653 Kaiserslautern, Germany | | FB Biologie/Abt. Zellbiologie Internet: nenno@rhrk.uni-kl.de | ---------------------------------------------------------------- From owner-chromosomes@net.bio.net Sun Oct 16 23:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!news.unige.ch!divsun!mike From: mike@divsun.unige.ch (Mike Morris) Subject: Re: Laboratory Management Systems Message-ID: <1994Oct17.165921.10739@news.unige.ch> Sender: usenet@news.unige.ch Organization: University of Geneva, Switzerland References: <1994Oct14.203737.16624@rhrk.uni-kl.de> Date: Mon, 17 Oct 1994 16:59:21 GMT Lines: 18 I too am looking actively at this problem (finding a genetics lab management package), and am finding it hard to locate existing packages. I am currently talking with Craig Rudlin of Medical Software and Computer Systems Inc, Richmond VA, fax 001.804.353.3418, who has a good-sounding package for Suns. The program is designed for running an endocrinology lab, but sounds modifiable for a genetics lab. We are talking about exactly what I require, which he can then build in. It may be worth getting in touch with him (if you do, please say I gave you his address - we have not yet fixed a price! ;) Mike ******************************************************************* Michael Morris PhD Division of Medical Genetics tel (Switzerland) (22) 702.56.94 CMU, University of Geneva fax (Switzerland) (22) 702.57.06 Geneva, Switzerland email mike@medsun.unige.ch From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!UKCC.UKY.EDU!VSC003 From: VSC003@UKCC.UKY.EDU (Ernest Bailey) Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 18 Oct 1994 21:54:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199410190454.VAA12029@net.bio.net> NNTP-Posting-Host: net.bio.net subscribe From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!news.ohsu.edu!sheaj From: sheaj@ohsu.edu (Jackson Shea) Newsgroups: bionet.genome.chromosomes Subject: Response to ALF Discussion group Date: 19 Oct 1994 16:48:35 GMT Organization: Oregon Health Sciences University Lines: 44 Message-ID: <383il3$2fc@steele.ohsu.edu> NNTP-Posting-Host: 137.53.10.2 Contribution to ALF discussion group In order to fill the gap created by Jackson Shea's call for contributions, I would like to take up the two points he suggested. 1. Mini-Preps. I believe that the best mini-prep method is the one that gives the best sequence; meaning, that the quality of DNA is not necessarily method-dependent but has more to do with the person preparing the DNA. In our core facility, I have had samples from ordinary good old alkaline-lysis giving super sequences, while some people can't even get sequenceable DNA from CsCl gradients. I still recommend PEG pptn.(when asked) because relatively little can go wrong, but I have no control about what is finally submitted for sequencing. I know many people are successful with Qiagen or similar resins while others manage to get residual resin (e.g. StrataClean) in their samples, which knocks out the DNA polymerase. We have had fewer quality problems since changing to cycle sequencing as our routine sequencing method. 2. Other ALF applications. A recent issue of BioTechniques contained an article from the Ansorge group abou the use of ALF for DNA footprinting. Another application that I know has been documented is primer extension analysis. I am, at the moment, adding the finishing touches to a method for fluorescence labelling of natural RNA (as opposed to in vitro transcripts) and have used this for looking at RNA/protein interactions by band shift gels on ALF. Unfortunately, Alf is not designed to enable cooling of the gels so that such gel-retardation experiments at aprox 30 C may miss weak interactions. For this purpose, our EMBL-ALF prototype is proving more flexible. Finally, may I blow my own trumpet by reminding ALF users of their favourable position (compared to ABI users, without wishing to start a major conflict) in being more readily able to use labelled primers in primer walking projects. We do not have to rely on the use of dye terminators, which, I have been told are expensive and give relatively short reads - (by the way, has anyone used dye terminators on ALF; and if so where does one get the fluorescein-labelled terminators from ?) - but have the option of using fluorescent phosphoramidites in primer synthesis or internal labelling with FdA. Alternatively, and paricularly for cycle sequencers, I would like point out the possibilty of the enzymatic addition of the fluorescent label to pre-existing primers (BioTechniques 15, 486-497, 1993). From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!lyra.csx.cam.ac.uk!doc.ic.ac.uk!charlie.lif.icnet.uk!mac034006.edin.icnet.uk!user From: h_gabra@icrf.icnet.uk (Hani Gabra) Newsgroups: bionet.genome.chromosomes Subject: Re: RDA Followup-To: bionet.genome.chromosomes Date: 19 Oct 1994 14:56:36 GMT Organization: Imperial Cancer Research Fund Lines: 18 Distribution: world Message-ID: References: NNTP-Posting-Host: mac034006.edin.icnet.uk In article , fsc@ARUBA.NYSAES.CORNELL.EDU (Frank Cheng) wrote: > > Has anyone out there tried representational difference analysis (RDA) in > order to find probes useful for genetic studies? Is that possible to > isolate genes by cloning the differences between two genomes? Are there > any references except the original paper (Science 259:946-951)? > There is a recent paper from Eric Lander in nature genetics on genetically directed RDA (GDRDA) and you can get a very informative and detailed protocol if you write to Drs Lisitsyn and Wigler at Cold Spring Harbor Best of Luck Hani Gabra ICRF Medical Oncology Unit Edinburgh,UK From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!convex!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!dsinc!ub!newserve!news.cc.geneseo.edu!news.cc.geneseo.edu!eg95 From: eg95@news.cc.geneseo.edu (Efram Gonzalez) Newsgroups: bionet.genome.chromosomes Subject: PCR PRimers Date: 18 Oct 1994 20:01 EST Organization: SUNY Geneseo Lines: 7 Distribution: world Message-ID: <18OCT199420010477@news.cc.geneseo.edu> NNTP-Posting-Host: uno.cc.geneseo.edu News-Software: VAX/VMS VNEWS 1.41 Hello. I would like to know if anyone could tell me what the criteria is for the creation of oligonucleotide primers for PCR. What makes a good primer? Are there any computer programs that can help create primers? Thanks in advance. From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!sol.ctr.columbia.edu!newsxfer.itd.umich.edu!europa.eng.gtefsd.com!uhog.mit.edu!news.mtholyoke.edu!news.umass.edu!news2.near.net!das-news2.harvard.edu!husc-news.harvard.edu!lipid!robison Newsgroups: bionet.genome.chromosomes Subject: Re: Nontraditional Inheritance Message-ID: <1994Oct19.002422.35255@hulaw1.harvard.edu> From: robison@lipid.harvard.edu (Keith Robison) Date: 19 Oct 94 00:24:20 EDT References: <36q2ak$k7r@vixen.cso.uiuc.edu> Nntp-Posting-Host: lipid.harvard.edu X-Newsreader: TIN [version 1.2 PL2] Lines: 47 Belinda J.F. Rossiter (rossiter@bcm.tmc.edu) wrote: : In article <36q2ak$k7r@vixen.cso.uiuc.edu>, dstone@prairienet.org (David : M. Stone) wrote: : > I recently received a grant for a teacher update workshop. : > I am interested in having a number of speakers present regarding : > a number of topics. Can anyone tell me specifically which topics : > fit under the "umbrella" of nontraditional inheritance? The already mentioned trinucleotide repeat expansion concept is a good one. It is nontraditional because the severity of the disease can increase with each transmission of the disease allele. This is due to the expansion of the unstable repeat (there is a pre-explanation term for this phenomenon which escapes me). Other candidates for "nontraditional inheritance" 1) Mitochondrial inheritance -- only from the mother in humans. There are several genetic diseases associated with mitochondrial genetic defects. Mitochondria are energy-producing organelles with their own genome, and are inherited only from the mother in the vast majority of cases. 2) Imprinting Imprinting refers to a difference in a gene's effect dependent on which parent it was inherited from. In some cases, genetic diseases occur in heterozygous individuals, because the locus is imprinted and the individual has the bad luck to inherit the functional copy from the parent whose contribution is turned off. 3) Uniparental disomy In some rare cases, a karyotypically-normal individual inherits both copies of a chromosome (or a large part of a chromosome) from the same parent. I'm sure someone out there can supply more... Keith Robison Harvard University Department of Cellular and Developmental Biology Department of Genetics / HHMI robison@mito.harvard.edu From owner-chromosomes@net.bio.net Tue Oct 18 23:00:00 1994 Path: biosci!news.ohsu.edu!sheaj From: sheaj@ohsu.edu (Jackson Shea) Newsgroups: bionet.genome.chromosomes Subject: dye-terminators and sequencing Date: 19 Oct 1994 22:02:26 GMT Organization: Oregon Health Sciences University Lines: 39 Sender: maryic@imgen.bcm.tmc.edu (Mary Irene Coolbaugh) Message-ID: <38451i$t30@steele.ohsu.edu> NNTP-Posting-Host: 137.53.10.2 Hi users... In response to the questiuons/issues raised by "igloi@oligo..." 1] Single-fluor (FAM) ddNTP's are available from duPont/NEN. I've used them on the ALF - but not for traditional sequencing, for alternative method development.... and yes, they showed up, very nicely. 2] Talk to Pharmacia about how-to-do modifications on the ALF to run water through a chiller and thus run the machine at 4-10 degrees C. This is how we do SSCP on the ALF. 3] Internal labeling w/ FdATP works well _if_ you have an even - or high - level of "A's" in the region to be sequenced. And if you have enough template to use T7 DNA polymerase. If you have a GC rich/ repeated region to analyze, there are probably less stressful approaches than to try to use FdATP. _Now_ - for my question of the week.... has anyone used, successfully, ABI's dye-terminator kit for ss DNA to do solid-phase [bead] sequencing of genomic DNA's - to detect heterozygousities? I get _great_ results on the ALF, but I have a three-week backlog, so "the powers-that-be" would like to try things on the ABI...higher thru-put and all....[Sorry, Casey and Trace] There is supposed to be a paper that addresses this very issue in "Methods in Enzymology" vol 218(?), by Schofield, Jones, and Vaudin. I've looked in 7 libraries at the TMC and several departmental conference rooms for this volume and paper, but to no avail. If anyone has access to this paper and/or personal experience with *dd genomic sequencing on the ABI please contact me via FAX or email: Inet:maryic@imgen.bcm.tmc.edu (that's in Texas, CDT, y'all.) FAX:713.798.7383 voice:713.798.6548 Thank you very much, Mary Coolbaugh Murphy {sorry about sending this through you, Jackson, we've had a lot of flooding around the Med Center and some of our net functions are still a little "chaotic"...things like trying to post to net-news, etc.....} From owner-chromosomes@net.bio.net Wed Oct 19 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!cs.utexas.edu!news.tamu.edu!news From: ahp2343@bioch.tamu.edu (Andrew H Paterson) Newsgroups: bionet.genome.chromosomes Subject: Postdoctoral Opportunities in Plant Molecular Genetics Date: 20 Oct 1994 02:32:07 GMT Organization: Texas A&M University, College Station, TX Lines: 46 Sender: -Not-Authenticated-[9418] Message-ID: <384kr7$dej@news.tamu.edu> NNTP-Posting-Host: sorghum.tamu.edu X-Posted-From: InterNews 1.0.1@sorghum.tamu.edu Xdisclaimer: No attempt was made to authenticate the sender's name. Two Postdoctoral Opportunities are available in my lab, for highly-motivated individuals to energetically pursue ongoing projects in Arabidopsis and Gossypium (cotton), respectively. The positions are described as follows: Arabidopsis Molecular Genetics: My lab has recently published QTL maps of flowering time and related traits (MGG, in press), and a comparative map of Arabidopsis and Brassica (Genetics, in press). I seek a well-qualified individual to pursue continuing reseach in one or both of these areas, and possibly other areas to be discussed. Qualifications should include Ph.D. in molecular genetics-related discipline, with comfort in contemporary genome analysis techniques, in particular experience in megabase DNA manipulation and cloning (BACs and/or YACs). Position is available immediately, and is funded for 2 years from 31 Aug 1994. Salary commensurate with experience, competitive benefits. Respond to address below. Cotton Molecular Genetics: My lab has recently published the first detailed molecular map of the cotton chromosomes (Genetics, in press), and has additional studies of genome organization, evolution, and transmission genetics in preparation. A well qualified individual is sought to pursue continuing research in one or more of these areas, and possibly other areas to be discussed. Qualifications should include Ph.D. in molecular genetics-related discipline, with comfort in contemporary techniques for DNA manipulation and cloning, including automated DNA sequencing and related data analysis. Prefer individual with experience in analysis of repetitive DNA elements. Position is available 1 Jan 1995 (possibly sooner), and is funded for 2 years from 31 Aug 1994. Salary commensurate with experience, competitive benefits. Respond to address below. Send (email, FAX, or otherwise) credentials, including detailed cv and names and telephone numbers of at least three professional references, to Andrew H. Paterson, Plant Genome Mapping Lab, Dept Soil and Crop Sciences, Texas A&M University, College Station, TX 77843-2474. Phone 1-409-845-3773; FAX 1-409-845-0456; EMAIL ahp2343@bioch.tamu.edu. Texas A&M University is an equal opportunity employer. From owner-chromosomes@net.bio.net Tue Oct 25 22:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!EU.net!uknet!festival!leeds.ac.uk!news From: ianf@epid.leeds.ac.uk (Ian Findlay) Subject: ALF Vs ABI Gene sequencer Message-ID: <1994Oct26.145311.5258@leeds.ac.uk> Sender: news@leeds.ac.uk Organization: Epidemiology Date: Wed, 26 Oct 1994 14:53:11 +0000 (GMT) X-Newsreader: WinVN 0.92.1 Lines: 14 Hi, I've just found this group and since I am using an ABI genescanner will I get kicked off. Or get any replies? Which is better, ALF or ABI. I am working on DNA fingerprinting of single-cells using fluorescent PCR. Is anyone else? Please email and tips, news etc. Ian From owner-chromosomes@net.bio.net Wed Oct 26 22:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!gatech!howland.reston.ans.net!EU.net!uknet!festival!leeds.ac.uk!news From: ianf@epid.leeds.ac.uk (Ian Findlay) Subject: ALF Vs ABI x2 Message-ID: <1994Oct27.204417.14639@leeds.ac.uk> Sender: news@leeds.ac.uk Organization: Epidemiology Date: Thu, 27 Oct 1994 20:44:16 +0000 (GMT) X-Newsreader: WinVN 0.92.1 Lines: 20 I have already has some response from my earlier mailing. It has been suggested that ALF is better than the ABI system but I don't know. I can very accuratly and very very reliably detect a single copy from a single cell. I can also multiplex upto 8 primer sets within a single cell. Both of these are using the ABI system Would the ALF system be any better. I have no commercial connection of any kind - just curious. I look forward to receiving any replies. Thanks Ian Please either mail me direct or to this group.