From owner-chromosomes@net.bio.net Thu Dec 01 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!europa.eng.gtefsd.com!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!homer15.u.washington.edu!mylerpj From: Peter Myler Newsgroups: bionet.genome.chromosomes Subject: Re: RARE technique; Rec-A mediated chromosome cleavage Date: Fri, 2 Dec 1994 12:06:22 -0800 Organization: University of Washington Lines: 28 Message-ID: References: NNTP-Posting-Host: homer15.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: On 1 Dec 1994, John J Weiland wrote: > > Can someone please post or mail me a reference for the use of RecA in the > blocking of methylation sites on DNA? I think the technique is > called RARE, and was mentioned in the recent Promega Notes with > regard to their Rec A product. Thanks in advance! > > John Weiland jweiland@badlands.nodak.edu > > I believe the reference is: Ferrin, L.J. and Camerini-Otero, R.D. (1991) Selective cleavage of human DNA: RecA-assisted restriction enzyme (RARE) cleavage. Science. 254:1494-1497. Regards Peter =============================================================================== Peter J. Myler phone: (206) 284-8846x332 Seattle Biomedical Research Institute FAX: (206) 284-0313 4 Nickerson Street e-mail: MYLERPJ@U.WASHINGTON.EDU Seattle, WA 98109-1651 =============================================================================== From owner-chromosomes@net.bio.net Thu Dec 01 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!torn!nott!ehd.hwc.ca!hpb.hwc.ca!dblakey From: dblakey@hpb.hwc.ca (D. BLAKEY) Newsgroups: bionet.genome.chromosomes Subject: CHO X chromosome Date: 2 Dec 1994 15:14:49 GMT Organization: Health Canada Lines: 7 Message-ID: <3bndla$ep6@m.ehd.hwc.ca> NNTP-Posting-Host: hpb.hwc.ca X-Newsreader: TIN [version 1.2 PL2] Does anyone out there know where I can get a probe for the distal part of the CHO X chromosome suitable for use in fluorescent in situ hybribization? Will be happy to give appropriate attribution on any resulting publications. Please reply to dblakey@hpb.hwc.ca David H. Blakey From owner-chromosomes@net.bio.net Sat Dec 03 22:00:00 1994 Path: biosci!cc.UManitoba.CA!gordonr From: gordonr@cc.UManitoba.CA (Richard Gordon) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosomal Organization Date: 4 Dec 1994 04:03:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 70 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net There may be some relationships between gene expression during embryonic development and the logical organization of the genome. To what extent this will reflect physical organization of the chromosomes is difficuly to determine. Consider a hierarchical Pascal computer program. What is its physical structure on your hard disk? If you knew the latter, what would it tell you about how the program works? Please see the appended request. Thanks, -Dick Gordon[Dec4,94] On 4 Dec 1994 hirshaut@yu1.yu.edu wrote: > > There is a great deal of effort to locate and characterize genes which > are related to specific functions or diseases. To support this process, > mapping of chromosomes appears to be making rapid progress. What, > however, is known about chromosome organization? It would seem logical > that genes with related functions share a single chromosome. Is there, > in fact, any information so far that confirms this assumption? If so, > any leads to which are the best studied chromosomes and who is working > most actively in this area. hirshaut@yu1.yu.edu. Thanks. > Dear Colleague: I am finishing a book about the intersection of three major fields of biology: Gordon, R. (1995). The Hierarchical Genome and Differentiation Waves: Novel Unification of Development, Genetics, and Evolution (Singapore: World Scientific), in prep. and refer to your work. I would appreciate reprints or preprints as soon as possible, or an update, if I've been in contact with you previously. Please be sure to include your e-mail address in case I have any questions. Thanks for your help. Best regards, -Dick Gordon Please mail to: Dr. Richard Gordon, Department of Radiology University of Manitoba, ON104, Health Sciences Centre 820 Sherbrook Street Winnipeg, Manitoba, Canada R3A 1R9 E-mail: GordonR@cc.UManitoba.ca Fax: (204) 783-8565 If you are curious, condensed accounts are given in: Gordon, R. & G.W. Brodland (1987). The cytoskeletal mechanics of brain morphogenesis: cell state splitters cause primary neural induction. Cell Biophysics 11, 177-238. Gordon, R. (1993). The fractal physics of biological evolution. In: Beysens, D., N. Boccara & G. Forgacs, eds. Dynamical Phenomena at Interfaces, Surfaces and Membranes. Commack, N.Y.: NOVA Science Publishers, 99-111. Bjorklund, N. K. & R. Gordon (1993). Nuclear state splitting: a working model for the mechanochemical coupling of differentiation waves to master genes (with an Addendum). Russian J. Dev. Biol. 24(2), 79-95. Gordon, R., N. K. Bjorklund & P. D. Nieuwkoop (1994). Dialogue on embryonic induction and differentiation waves. Int. Rev. Cytol. 150, 373-420. Bjorklund, N. K. & R. Gordon (1994). Surface contraction and expansion waves correlated with differentiation in axolotl embryos. I. Prolegomenon and differentiation during the plunge through the blastopore, as shown by the fate map. Computers & Chemistry 18(3), 333-345. From owner-chromosomes@net.bio.net Sat Dec 03 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!pipex!uunet!psinntp!alsys1!yu1.yu.edu!hirshaut From: hirshaut@yu1.yu.edu Newsgroups: bionet.genome.chromosomes Subject: Chromosomal Organization Message-ID: Date: 4 Dec 94 02:05:36 GMT Sender: news@alsys1.aecom.yu.edu Lines: 14 Nntp-Posting-Host: yu1.yu.edu MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII There is a great deal of effort to locate and characterize genes which are related to specific functions or diseases. To support this process, mapping of chromosomes appears to be making rapid progress. What, however, is known about chromosome organization? It would seem logical that genes with related functions share a single chromosome. Is there, in fact, any information so far that confirms this assumption? If so, any leads to which are the best studied chromosomes and who is working most actively in this area. hirshaut@yu1.yu.edu. Thanks. From owner-chromosomes@net.bio.net Mon Dec 05 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!news2.near.net!das-news2.harvard.edu!fas-news.harvard.edu!lipid!robison From: robison@lipid.harvard.edu (Keith Robison) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosomal Organization Date: 6 Dec 1994 04:27:46 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 39 Message-ID: <3c0p82$58r@decaxp.harvard.edu> References: NNTP-Posting-Host: lipid.harvard.edu X-Newsreader: TIN [version 1.2 PL2] hirshaut@yu1.yu.edu wrote: : There is a great deal of effort to locate and characterize genes which : are related to specific functions or diseases. To support this process, : mapping of chromosomes appears to be making rapid progress. What, : however, is known about chromosome organization? It would seem logical : that genes with related functions share a single chromosome. Is there, : in fact, any information so far that confirms this assumption? If so, : any leads to which are the best studied chromosomes and who is working : most actively in this area. hirshaut@yu1.yu.edu. Thanks. There do exist clusters of genes with related function -- many components of the immune system are clustered in the Major Histocompatibility Complex. However, the trend seems to be that genes with related function are only clustered if they are directly related by duplication. For example, the HOX genes occur in nice little clusters whose structure is largely conserved between flies and man. But the HOX genes are all related to each other by duplication. In prokaryotes related genes are clustered into operons. Last year there was the identification of an operon in C.elegans, and a recent Nature paper identified many more operons in C.elegans. However, C.elegans appears to be very odd in this way amongst the eukaryotes. Try searching Medline or Current Contents with "operon and elegans" or "histocompatibility and organization". You might also look in Stephen O'Brien's papers on genome segments conserved between different vertebrates -- there might be info there on clustering. Keith Robison Harvard University Department of Cellular and Developmental Biology Department of Genetics / HHMI robison@mito.harvard.edu From owner-chromosomes@net.bio.net Mon Dec 05 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!lll-winken.llnl.gov!unixhub!news.Stanford.EDU!ladasky From: ladasky@leland.Stanford.EDU (John Ladasky) Newsgroups: bionet.immunology,bionet.genome.chromosomes Subject: Cell Surface Marker for Human Chromosome 15? Date: 6 Dec 1994 23:16:28 GMT Organization: Stanford University, CA 94305, USA Lines: 16 Message-ID: <3c2rcc$rvl@nntp.Stanford.EDU> NNTP-Posting-Host: elaine42.stanford.edu Xref: biosci bionet.immunology:2571 bionet.genome.chromosomes:356 Greetings, everyone, I'm thinking about trying to select for/against the presence of human chromosome 15 in a hybridoma. It would be really handy if there was a cell surface protein expressed on this chromosome which I could identify with an antibody. Is there a database that lists genes and their location in the genome? I could perform a simple database search if this information is available. Thanks in advance! -- Unique ID : Ladasky, John Joseph Jr. Title : BA Biochemistry, U.C. Berkeley, 1989 Location : Stanford University, Dept. of Structural Biology, Fairchild D-105 Keywords : immunology, music, running, Green From owner-chromosomes@net.bio.net Tue Dec 06 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!panix!zip.eecs.umich.edu!newsxfer.itd.umich.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!root From: dadler@u.washington.edu (David Adler) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosomal Organization Date: 7 Dec 1994 04:31:07 GMT Organization: University of Washington Lines: 30 Message-ID: <3c3dqb$f0l@news.u.washington.edu> References: <3c0p82$58r@decaxp.harvard.edu> Reply-To: dadler@u.washington.edu NNTP-Posting-Host: koko.pathology.washington.edu > hirshaut@yu1.yu.edu wrote: > > : ...What, however, is known about chromosome organization? > : It would seem logical that genes with related functions share > : a single chromosome. Is there, in fact, any information > : so far that confirms this assumption?... and, In article <3c0p82$58r@decaxp.harvard.edu> robison@lipid.harvard.edu (Keith Robison) writes: > However, the trend seems to be that genes with related function are only > clustered if they are directly related by duplication. For example, > the HOX genes occur in nice little clusters whose structure is largely > conserved between flies and man. But the HOX genes are all related to > each other by duplication. But even more interesting, I think, and not explained by the duplication origin of the homeotic clusters, is the situation of the fly Ant/bithorax cluster and its mouse homolog. Within these clusters the order of the individual genes on the chromosome (map) is paralleled by the order of expression, anterior-posterior, of these genes in the embryo. And, this parallel relationship between ordered chromosomal arrangement and spatial expression pattern is conserved from fly to mouse! Curious, yes? -- David A. Adler Pathology SM-30 University of Washington Seattle, WA 98195 (206) 543-0716 (phone) (206) 543-3644 (fax) "Science is nothing but trained and organized common sense" T.H.Huxley From owner-chromosomes@net.bio.net Wed Dec 07 22:00:00 1994 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!zip.eecs.umich.edu!umn.edu!newsdist.tc.umn.edu!urvile.msus.edu!TIGGER.STCLOUD.MSUS.EDU!POWERJ01 From: powerj01@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.genome.chromosomes Subject: Telomerase Date: 7 Dec 1994 16:19:03 GMT Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 3 Message-ID: <3c4n9n$lu2@urvile.MSUS.EDU> Reply-To: powerj01@TIGGER.STCLOUD.MSUS.EDU NNTP-Posting-Host: tigger.stcloud.msus.edu I am currently involved in a readings in biology class where I will be researching telomerase and its effects on ageing and cancer. I have been having some poor luck finding much written about this. Can anyone share some information on this subject or point me in the right direction? E-mail me at powerj01@tigger.stcloud.msus.edu or respond to this post. thank you for any help you can afford me. From owner-chromosomes@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!aggedor.rmit.EDU.AU!minyos.xx.rmit.EDU.AU!s883730 From: s883730@minyos.xx.rmit.EDU.AU (Andrew Leslie Saul) Newsgroups: bionet.genome.chromosomes Subject: cyclophosphamide and skeletal development Date: 9 Dec 1994 04:04:56 GMT Organization: Royal Melbourne Institute of Technology, Melbourne, Australia. Lines: 8 Message-ID: <3c8l18$ndo@aggedor.rmit.EDU.AU> NNTP-Posting-Host: minyos.xx.rmit.edu.au I am trying to find literature on genotoxins which alter the genetic structure on bone cells, causing skeletal deformities. I have been told cyclophosphamide may be such a genotoxin? Anyone with info please reply. cheers Andrew alf@bunyip.ph.rmit.edu.au From owner-chromosomes@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!swiss.ans.net!newstf01.news.aol.com!newsbf01.news.aol.com!not-for-mail From: aegmendenh@aol.com (AEGMendenh) Newsgroups: bionet.genome.chromosomes Subject: Re: *** Q: WHAT KIND OF PEOPLE ON THE NET ? Date: 9 Dec 1994 00:50:41 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: news@newsbf01.news.aol.com Message-ID: <3c8r7h$d5b@newsbf01.news.aol.com> References: <39b2dd$9of@anemone.saclay.cea.fr> NNTP-Posting-Host: newsbf01.news.aol.com In article <39b2dd$9of@anemone.saclay.cea.fr>, cisitm@albert.cad.cea.fr (Pierre Didierjean) writes: Reply: I am a secondary biology educator who wpecializes in human genetics and bioethics. Much of my time is spent training tachers in this area. Gordon Mendenhall From owner-chromosomes@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!uunet!newstf01.news.aol.com!newsbf01.news.aol.com!not-for-mail From: aegmendenh@aol.com (AEGMendenh) Newsgroups: bionet.genome.chromosomes Subject: Re: Telomerase Date: 9 Dec 1994 00:55:26 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: news@newsbf01.news.aol.com Message-ID: <3c8rge$d7d@newsbf01.news.aol.com> References: <3c4n9n$lu2@urvile.MSUS.EDU> NNTP-Posting-Host: newsbf01.news.aol.com In article <3c4n9n$lu2@urvile.MSUS.EDU>, powerj01@TIGGER.STCLOUD.MSUS.EDU writes: I cant help you but would apprecite any bibliography that you get in your search. thanks. Gordon Mendenhall From owner-chromosomes@net.bio.net Fri Dec 09 22:00:00 1994 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 10 Dec 1994 02:00:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 337 Sender: daemon@net.bio.net Distribution: world Message-ID: <199412101000.CAA19116@net.bio.net> Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us a complete new updated form. We can not make manual revisions to existing entries. 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For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis ASCB bionet.prof-society.ascb BIOCAN bionet.prof-society.cfbs BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIOPHYSICAL-SOCIETY bionet.prof-society.biophysics BIOPHYSICS bionet.biophysics BIO-SOFTWARE bionet.software BIOTHERMOKINETICS bionet.metabolic-reg BIO-WWW bionet.software.www CARDIOVASCULAR-RESEARCH bionet.biology.cardiovascular CELEGANS bionet.celegans CELL-BIOLOGY bionet.cellbiol CHLAMYDOMONAS bionet.chlamydomonas CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational CSM bionet.prof-society.csm CYTONET bionet.cellbiol.cytonet DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMF-BIO bionet.emf-bio EMPLOYMENT bionet.jobs EMPLOYMENT-WANTED bionet.jobs.wanted FASEB bionet.prof-society.faseb GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage GRASSES-SCIENCE bionet.biology.grasses HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MICROBIOLOGY bionet.microbiology MOLECULAR-EVOLUTION bionet.molbio.evolution MOLECULAR-MODELLING bionet.molec-model MOLLUSC-MOLECULAR-NEWS bionet.molbio.molluscs MYCOLOGY bionet.mycology NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PARASITOLOGY bionet.parasitology PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography PROTISTA bionet.protista RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources STADEN bionet.software.staden STRUCTURAL-NMR bionet.structural-nmr TROPICAL-BIOLOGY bionet.biology.tropical URODELES bionet.organisms.urodeles VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast ZBRAFISH bionet.organisms.zebrafish Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-chromosomes@net.bio.net Mon Dec 12 22:00:00 1994 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!usc!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!nuclear.microserve.net!evitts.microserve.com!user From: mevitts@evitts.microserve.com Newsgroups: bionet.genome.chromosomes Subject: Genetics books for laymen Date: 13 Dec 1994 01:54:22 GMT Organization: Microserve Information Systems (800)-380-INET Lines: 2 Distribution: world Message-ID: NNTP-Posting-Host: evitts.microserve.com I'm interested in a book or books on genetics that explains the basics.If it had a chapter on gene mutations this would also be helpful. Thanks. From owner-chromosomes@net.bio.net Mon Dec 12 22:00:00 1994 Path: biosci!newshost.lanl.gov!news.ttu.edu!aurora.LaTech.edu!darwin.sura.net!ukma!occ!ndlc.occ.uky.edu!daviseg From: daviseg@ndlc.occ.uky.edu (Eric Davis) Newsgroups: bionet.genome.chromosomes Subject: BIOLOGY IMAGES Date: 12 Dec 1994 22:35:41 GMT Organization: The NDLC's Internet Gateway Lines: 5 Message-ID: <3cij7t$ac6@sparc.occ.uky.edu> NNTP-Posting-Host: ndlc.occ.uky.edu X-Newsreader: TIN [version 1.2 PL2] Does anyone know where I could find GIF's or JPEG's of biology-related images? Thank you. Eric Davis From owner-chromosomes@net.bio.net Mon Dec 12 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!root From: David Adler Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics books for laymen Date: 13 Dec 1994 05:18:47 GMT Organization: University of Washington Lines: 20 Message-ID: <3cjarn$ch2@news.u.washington.edu> References: NNTP-Posting-Host: graphx.pathology.washington.edu mevitts@evitts.microserve.com wrote: > > I'm interested in a book or books on genetics that explains the basics.If > it had a chapter on gene mutations this would also be helpful. Thanks. Author: Gonick, Larry. Wheelis, Mark Title: The Cartoon Guide to Genetics Publisher: HarpC 08/1991 Year: 1991 Price: pap. $12.00 ISBN: 0062730991 The best introduction to genetics I know of, cartoons or no cartoons! Have fun... David -- David Adler University of Washington, Pathology Seattle From owner-chromosomes@net.bio.net Wed Dec 14 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!uhog.mit.edu!europa.eng.gtefsd.com!howland.reston.ans.net!pipex!sunsite.doc.ic.ac.uk!cpca3.uea.ac.uk!news From: Richard James Newsgroups: bionet.genome.chromosomes Subject: Re: BIOLOGY IMAGES Date: 15 Dec 1994 13:44:32 GMT Organization: University of East Anglia, Norwich, Norfolk, NR47TJ, UK Lines: 18 Message-ID: <3cph80$hd4@cpca3.uea.ac.uk> References: <3cij7t$ac6@sparc.occ.uky.edu> NNTP-Posting-Host: biorij.bio.uea.ac.uk daviseg@ndlc.occ.uky.edu (Eric Davis) wrote: > > Does anyone know where I could find GIF's or JPEG's of biology-related > images? Thank you. Try logging on to the Protein Data Bank WWW page at the address http://www.pdb.bnl.gov using a reader like Mosaic Netscape. Select the option "Selected images of entry files" Regards, Richard James From owner-chromosomes@net.bio.net Thu Dec 15 22:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!utcsri!newsflash.concordia.ca!news.mcgill.ca!clouso.crim.ca!athena.ulaval.ca!ppp9.ulaval.ca!user From: Luc.Simon@rsvs.ulaval.ca (Luc Simon) Subject: Alternate ABI373 seq. protocols? Message-ID: Sender: news@athena.ulaval.ca Nntp-Posting-Host: ppp9.ulaval.ca Organization: Universite Laval Date: Fri, 16 Dec 1994 03:15:49 GMT Lines: 22 We have been operating a DNA sequencing core facility with an ABI 373 for a couple of months now and are always using the Taq DyeDeoxy protocols. We generally have nice reads, but sometimes stumble on problematic templates. For instance, mostly on PCR fragments, we will get very nice reads with one primer, but no readable sequence with the other primer, using the same template (both primers are the same that were used to obtain the PCR fragment). Our hypothesis is that secondary structures hinder the sequencing reaction on the problematic strand. Realizing that since the initial PCR worked in the first place, the problem might be with the lower extension temperature recommended in the ABI protocol (60C for DyeDeoxy sequencing instead of usual 72C for regular Taq PCR). Questions: Has anybody tried to sequence with ABI DyeDeoxy kits at higher extension temperature? Any suggestions for improving our results in such cases? Thanks for sharing your experience, we will summarize the answers received From owner-chromosomes@net.bio.net Thu Dec 15 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!swiss.ans.net!prodigy.com!usenet From: LVWM59A@prodigy.com (Joseph Simpson) Newsgroups: bionet.genome.chromosomes Subject: Chromasome22 Date: 16 Dec 1994 12:07:31 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 6 Distribution: world Message-ID: <3crvu3$rki@usenetw1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 I am looking for any laymen lterature on the 22nd chromasome. After 5 years and many doctors my son has been diagnosed as missing part of his. especially any case histories and/or treatments would be helpful. Joseph E, Simpson ( LVWM59A@Prodigy.com) From owner-chromosomes@net.bio.net Fri Dec 16 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: Autoseq news Date: 17 Dec 1994 11:11:27 -0000 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3cuh0v$s62@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: pluto: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Dear autosequencers , I am trying to establish if there is any intrest out there in an autosequence newsgroup , solely devoted to helping solve the problem inherent with advancing technologies such as the ones we are involved with . The newsgroup would of course help to form better links between users regardless of which type of machine you use , or wether you run a core facilty or simply use autosequencing to improave your own experiments . I feel that we need to concentrate our resourses and be able to take part in informative discussions without having to sift through the number of other diverse messages that go to a newsgroup such as this . Topic that i have in mind include comparisons of template preperation , techniques for sequencing through difficult templates and input into how users are finding the new advances in robotic assistance . If you support my call for a dedicated newsgroup please email me at : cain@icr.ac.uk If there is sufficient support we can go ahead so please bring this to the attention of other intrested parties that you know and whom may not be a member of this newsgroup . Thankyou for your co-operation and have a great Xmas , David Cain Automated DNA sequencing facility Institute of cancer research London From owner-chromosomes@net.bio.net Fri Dec 16 22:00:00 1994 Path: biosci!AARDVARK.UCS.UOKNOR.EDU!BROE From: BROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe) Newsgroups: bionet.genome.chromosomes Subject: Re: Autoseq news Date: 17 Dec 1994 05:39:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HKQQ498QUQ0006O7@aardvark.ucs.uoknor.edu> NNTP-Posting-Host: net.bio.net You write: => Dear autosequencers , => => I am trying to establish if there is any intrest out there in an => autosequence newsgroup , solely devoted to helping solve the problem inherent => with advancing technologies such as the ones we are involved with . The => newsgroup would of course help to form better links between users regardless => of which type of machine you use , or wether you run a core facilty or simply => use autosequencing to improave your own experiments . I started one several years ago as an e-mail list that evolved into this news group. So this is the place to post these messages and discuss them. => I feel that we need to concentrate our resourses and be able to take => part in informative discussions without having to sift through the number of => other diverse messages that go to a newsgroup such as this . The Biochrom news group does not have that many messages to sift through so go ahead and use this news group for discussing the topics you mention. => Topic that i have in mind include comparisons of template preperation => , techniques for sequencing through difficult templates and input into how => users are finding the new advances in robotic assistance . => If you support my call for a dedicated newsgroup please email me at : => => cain@icr.ac.uk => => If there is sufficient support we can go ahead so please bring this => to the attention of other intrested parties that you know and whom may not be => a member of this newsgroup . => Thankyou for your co-operation and have a great Xmas , => => David Cain => Automated DNA sequencing facility => Institute of cancer research => London This is the news group to use, it already exists, tell everyone and go for it. Merry Christmas and Happy New Year. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - \ Bruce A. Roe Professor of Chemistry and Biochemistry / / University of Oklahoma INTERNET: BROE@uoknor.edu \ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - From owner-chromosomes@net.bio.net Fri Dec 16 22:00:00 1994 Path: biosci!darwin.com!mulligan From: mulligan@darwin.com ("Mulligan, John") Newsgroups: bionet.genome.chromosomes Subject: Template preparations Date: 17 Dec 1994 11:45:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412171943.AA16677@uu6.psi.com> NNTP-Posting-Host: net.bio.net Anyone interested in a thread on template preparation for sequencing? We have been trying the Qiagen 96-well M13 purification plates, and have found that although the yeild is very low ( <25% of that acheived by quantitative methods such as phenol extraction ) the quality of the template is good and consistant. The cost is high, but balanced by the savings in labor and the potential for automated handling. Is anyone using these? Any experience with yield, or tips for greater recovery? Is anyone using mag beads in high throughput applications? Any other solid phase schemes you are willing to talk about? We are using 96-well spin columns to purify PCR products for sequencing (as described by Francois Iris (in Automated DNA sequencing and Analysis) and Kai Wang (personal communication). Yield of DNA is good and reliable, something aroud 80% of the templates sequence well. Any other schemes for sequencing PCR templates in use? What are you success rates? Is anyone using the no-purification protocol that Lin Zuo presented at the last Cold Spring Harbor meeting? (This scheme may depend on access to the new mutant Taq that ABI will be relaesing in the near future). John Mulligan Director of Sequencing Darwin Molecular Corporation From owner-chromosomes@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!CSHL.ORG!lodhi From: lodhi@CSHL.ORG (Muhammad Lodhi) Newsgroups: bionet.genome.chromosomes Subject: Re: Autoseq news Date: 19 Dec 1994 10:44:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412191842.AA25555@phage.cshl.org> NNTP-Posting-Host: net.bio.net >In article <01HKQQ498QUQ0006O7@aardvark.ucs.uoknor.edu>, >BROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe) wrote: > >> You write: >> => Dear autosequencers , >> => >> => I am trying to establish if there is any intrest out there in an >> => autosequence newsgroup > >> I started one several years ago as an e-mail list that evolved into this >> news group. So this is the place to post these messages and discuss them. >> >> This is the news group to use, it already exists, tell everyone and go for >> it. Merry Christmas and Happy New Year. >> >> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - >> \ Bruce A. Roe Professor of Chemistry and Biochemistry / >> / University of Oklahoma INTERNET: BROE@uoknor.edu \ >> - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - > > And yet most of the autosequencing postings seem to go to >bionet.molbio.methds-reagnts rather than to here. Maybe the phrase >"genome.chromosomes" does not sufficiently connote automated sequencing. >I know it took me a while to discover it, and I do automated sequencing >and read the newsgroups regularly. > Here's an idea. If we do not want to create a whole new group, how >about changing the name of bionet.genome.chromosomes to include >autosequencing? Otherwise I expect that many users will continue to post >to bionet.molbio.methds-reagnts because that title sounds more >appropriate. Just a thought. > >Lawrence Washington >Indiana Institute for Molecular and Cellular Biology > >-- >Lawrence Washington >Indiana University >Institute for Molecular > and Cellular Biology -------------------------------------------------------------------------------- I agree with Lawrence, as the name is misleading to some extend. I have been posting messages related with sequencing to the methods-reagents newsgroup. Muhammad A. Lodhi Cold Spring Harbor Lab. From owner-chromosomes@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!usenet.ucs.indiana.edu!raven.bio.indiana.edu!user From: lwashing@sunflower.bio.indiana.edu (Lawrence Washington) Newsgroups: bionet.genome.chromosomes Subject: Re: Autoseq news Date: 19 Dec 1994 17:59:21 GMT Organization: Indiana University Lines: 39 Distribution: world Message-ID: References: <01HKQQ498QUQ0006O7@aardvark.ucs.uoknor.edu> NNTP-Posting-Host: raven.bio.indiana.edu In article <01HKQQ498QUQ0006O7@aardvark.ucs.uoknor.edu>, BROE@AARDVARK.UCS.UOKNOR.EDU (Bruce Roe) wrote: > You write: > => Dear autosequencers , > => > => I am trying to establish if there is any intrest out there in an > => autosequence newsgroup > I started one several years ago as an e-mail list that evolved into this > news group. So this is the place to post these messages and discuss them. > > This is the news group to use, it already exists, tell everyone and go for > it. Merry Christmas and Happy New Year. > > - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - > \ Bruce A. Roe Professor of Chemistry and Biochemistry / > / University of Oklahoma INTERNET: BROE@uoknor.edu \ > - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - And yet most of the autosequencing postings seem to go to bionet.molbio.methds-reagnts rather than to here. Maybe the phrase "genome.chromosomes" does not sufficiently connote automated sequencing. I know it took me a while to discover it, and I do automated sequencing and read the newsgroups regularly. Here's an idea. If we do not want to create a whole new group, how about changing the name of bionet.genome.chromosomes to include autosequencing? Otherwise I expect that many users will continue to post to bionet.molbio.methds-reagnts because that title sounds more appropriate. Just a thought. Lawrence Washington Indiana Institute for Molecular and Cellular Biology -- Lawrence Washington Indiana University Institute for Molecular and Cellular Biology From owner-chromosomes@net.bio.net Sun Dec 18 22:00:00 1994 Newsgroups: bionet.genome.chromosomes Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!news.unige.ch!usenet From: mike@medsun.unige.ch (Mike Morris) Subject: ABI Taq Pol sequencing reactions Message-ID: <1994Dec19.122110.20021@news.unige.ch> Sender: usenet@news.unige.ch Reply-To: mike@medsun.unige.ch Organization: University of Geneva -Medical Center-, Switzerland Date: Mon, 19 Dec 1994 12:21:10 GMT Lines: 28 For our ABI373 sequencer, we currently go through the following protocol, which gives good consistent results: miniprep plasmids measure OD sequence (Taq Pol, dye primers) phenol chloroform precipitate ethanol wash run. The slowest part, and the bottleneck of the whole protocol, is the extraction/precipitation, which seriously limits our throughput. Could anybody give us tried-and-tested protocols for speeding this up? Even if the cost goes up (I have wondered about spin columns), the increase in throughput would make this worthwhile. Thanks in advance, Mike Morris ******************************************************************* Division of Medical Genetics tel (Switzerland) (22) 702.56.94 CMU, University of Geneva fax (Switzerland) (22) 702.57.06 Geneva, Switzerland email mike@medsun.unige.ch From owner-chromosomes@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!D.UMN.EDU!grad From: grad@D.UMN.EDU (stephen hedman) Newsgroups: bionet.genome.chromosomes Subject: Position Announcement Date: 19 Dec 1994 15:26:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199412192326.RAA03750@ub.d.umn.edu> NNTP-Posting-Host: net.bio.net Subject: Position Announcement The Department of Biology at the University of Minnesota-Duluth has a faculty position at the rank of instructor or assistant professor available for spring quarter 1995 (March 1 - May 30). The person hired will teach a molecular biology and genetics lecture course, with course content appropriate for a text such as Genes V by Lewin; and an established molecular biology and genetics laboratory course. An experienced graduate teaching assistant will be assigned to the laboratory course. Applicant must have at least A.B.D. in a relevant biological speciality, collegiate teaching experience, training in molecular genetics, and background and experience in bacterial and viral genetics and recombinant DNA techniques. Doctoral degree preferred. Application materials should include: letter of application; curriculum vitae; undergraduate and graduate transcripts (photocopies acceptable); names and telephone numbers of three references familiar with applicant's teaching and experience in molecular genetics. Applications must be postmarked by January 24, 1995 to: Donald P. Christian, Department of Biology, University of Minnesota, Duluth, MN 55812. Inquiries may be directed to D. Christian, telephone 218/726-7263, e-mail dchristi@ub.d.umn.edu. The University of Minnesota is an equal opportunity educator and employer. From owner-chromosomes@net.bio.net Mon Dec 19 22:00:00 1994 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!jobone!lynx.unm.edu!carina!inkim From: inkim@carina.unm.edu (In C. Kim) Newsgroups: bionet.genome.chromosomes Subject: Oligos vs. Chromosomes Date: 20 Dec 1994 18:40:10 GMT Organization: University of New Mexico, Albuquerque Lines: 12 Message-ID: <3d78ea$nrj@lynx.unm.edu> NNTP-Posting-Host: carina.unm.edu Keyword: X-Newsreader: TIN [version 1.2 PL2] Greetings! I have a very dumb question. Since I do not have a background in molecular genetics, this newsgroup seems to be the right place to raise a question. Many oligos for human chromosomes are readily available for PCR and in situ hybridization. I am wondering whether there is a web site(s) to visualize approximate binding sites of oligo probes to chromosomes (e.g., p, q, or centromere). Please briefly describe how to do it, if any. Thanks. In C. Kim From owner-chromosomes@net.bio.net Mon Dec 19 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!EU.net!sun4nl!uva.nl!amc.uva.nl!news From: bergen@amc.uva.nl Newsgroups: amc.algemeen,bionet.genome.chromosomes,b Subject: molecular genetics comm.list Date: Tue, 20 Dec 94 17:22:44 GMT Organization: Central Computer Organization AMC/UvA Lines: 41 Distribution: world Message-ID: <3d6ssk$ki0@amcnix.amc.uva.nl> NNTP-Posting-Host: amccca.amc.uva.nl Keywords: genetics,molecular biology ANNOUNCING : THE LARGEST AND FASTEST GROWING INTERNET COMMUNICATION LIST IN (HUMAN) MOLECULAR GENETICS Name list : HUM-MOLGEN Size : HUM-MOLGEN IS NOW (DEC 20,1994) A COMMUNICATION WEB OF APPROXIMATELY **100** INSTITUTES WORLDWIDE short description: Human Molecular Genetics long description : This list is open for discussions, noncommercial adds, announcements and questions related to the field of Molecular Genetics, with emphasis on human molecular genetics. Subscribers are (clinical) geneticist, molecular biologists, (medical or biology) students and other individuals interested in the field. Entry : International Administrator : Dr. A.A.B. Bergen, Bergen@amc.uva.nl Co-Administrator : Dr. F. Zollmann, Zollmann.1@osu.edu The list is located on: Listserv@nic.surfnet.nl Interested individuals can subscribe by sending E-mail to (Subscription is free of charge) Listserv@nic.surfnet.nl containing the message: subscribe HUM-MOLGEN first_name last_name and quit the message: signoff HUM-MOLGEN From owner-chromosomes@net.bio.net Tue Dec 20 22:00:00 1994 Path: biosci!HELIX.MGH.HARVARD.EDU!HAINES From: HAINES@HELIX.MGH.HARVARD.EDU ("Jonathan L. Haines") Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 21 Dec 1994 08:19:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 161 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HKWJ9SWBUA8XEWZ2@HELIX.MGH.HARVARD.EDU> NNTP-Posting-Host: net.bio.net ********************************* * COURSE ANNOUNCEMENT * ********************************* ---GENETIC ANALYSIS METHODS FOR MEDICAL RESEARCHERS--- Description: This intensive four day course centers on mapping human genetic diseases. The concentration is on the entire disease mapping process, including clinical classification, pedigree collection, molecular genetic analysis, statistical analysis, and gene characterization. The course emphasizes the global decision-making process, rather than details of specific techniques. A residential conference center setting promotes extensive interaction between the students and the internationally recognized faculty. Course goals: 1. To outline and instruct participants about the necessary steps and procedures used in ascertaining and collecting pedigree data and clinical and family history information. 2. To discuss in detail both the molecular and statistical methodologies for using the reference marker maps generated by the efforts of the Human Genome Initiative. Emphasis will be on working examples and the basic theory behind genetic mapping methodologies. 3. To educate participants on the general interpretation of linkage results for both simple and complex disorders. Discussions will include an overview of positional cloning strategies, refinement of the preliminary linkage data, investigation of power, examination of heterogeneity, physical mapping, cDNA analysis, exon amplification, etc. This course will not include any bench or "wet" laboratory experience. Co-organizers: Margaret A. Pericak-Vance, Ph.D., Department of Medicine, Department of Genetics, Duke University School of Medicine. She is a founding fellow of the American College of Medical Genetics (ABMS) and a board-certified Ph.D. medical geneticist with fourteen years experience in genetic counseling. Dr. Pericak-Vance is a genetic epidemiologist whose research has focused on mapping the human genome with emphasis on the mapping of genetic disorders, with more recent extensions into complex genetic disease. She has concentrated on neurogenetic diseases, actively mapping such disorders as familial amyotrophic lateral sclerosis (Lou Gehrig disease), tuberous sclerosis, and late onset Alzheimer disease (AD). She is a member of the Mammalian Genetics Study Section at NIH. Jonathan L. Haines, Ph.D., Department of Neurology, Massachusetts General Hospital, Harvard Medical School. Dr. Haines is a genetic epidemiologist whose research has focused on both human disease and general (reference) chromosome mapping. Dr. Haines has concentrated on neurogenetic diseases, including the mapping of familial amyotrophic lateral sclerosis, familial Alzheimer disease, Huntington disease, tuberous sclerosis, neurofi- bromatosis, and multiple sclerosis. Dr. Haines is presently an editor of chromosome 9 for the Genome Data Base (GDB). He is an associate editor of Genomics and an editor of Current Protocols in Human Genetics. Instructors: Arthur S. Aylsworth, M.D., Division of Genetics and Metabolism, Department of Pediatrics, The University of North Carolina at Chapel Hill. Dr. Aylsworth is a board-certified pediatrician and clinical geneticist. As a physician-scientist, Dr. Aylsworth plays primary roles in family ascertainment and phenotypic delineation. He is actively involved in studies on neurofibromatosis and neural tube defects. David Goldgar, Ph.D., Genetic Epidemiology, Department of Medical Informatics, University of Utah. Dr. Goldgar is a statistical geneticist with extensive experience in both the theoretical and practical aspects of mapping human quantitative and complex traits. He has concentrated on the genetic epidemiology of common cancers, focusing on the genetic mapping of breast and ovarian cancer. He is actively involved in several large pedigree ascertainment and collection studies. Deborah A. Meyers, Ph.D., Department of Medicine, The Johns Hopkins University. Dr. Meyers is a statistical geneticist who has concentrated her research on the genetic basis of complex disorders including allergy and psychiatric diseases such as schizophrenia and Alzheimer disease. She has extensive didactic experience in her teaching role at the short course in Medical and Experimental and Mammalian Genetics, The Jackson Laboratory, Bar Harbor, Maine. Jeffrey Murray, M.D., Department of Pediatrics, University of Iowa. Dr. Murray is a molecular biologist and physician scientist with extensive experience in both general chromosome and disease gene mapping. Dr. Murray is a board-certified pediatrician and clinical geneticist. His research interests include genetic studies in cleft-lip and palate. He also directs a national effort to generate a high resolution genetic map of the entire genome. Dr. Murray is a GDB Editor for chromosome 4 and a former member of the Mammalian Genetics Study Section at NIH. Marcy C. Speer, M.S., Ph.D., Department of Medicine, Duke University Medical School. Dr. Speer is a board-certified genetic counselor with eleven years of experience. She is also a genetic epidemiologist whose research interest is in mapping human genetic diseases, including the muscular dystrophies and neural tube defects. She is also involved in studies of unusual genetic phenomena such as anticipation and imprinting. Participation: Participation in the course will be dependent on completion of an application form that describes the applicant's background and research interests and will be limited to 36 students. All participants will need to show evidence of a postgraduate genetics course or its equivalent. Participants must provide a brief statement describing their research interests, their reason for taking the course, and their long-term objectives in relation to the course curriculum. This information will be used to select a highly motivated participant group. Minority and women applicants are specifically encouraged to apply. A limited number of scholarships are available for registered students or fellows. Scholarship selection will be based on the strength of the individual applications. Location: The course will be held at the R. David Thomas Center located on the campus of Duke University, Durham, NC. The Thomas Center is a new facility which establishes a climate of hospitality and an atmosphere conductive to learning and to the exchange of ideas. Both students and faculty will be housed at the R. David Thomas Center. Date: March 25-29, 1995 Deadline for completed application: January 2, 1995 For information and application forms contact: Write: Genetic Methods Course c/o Dr Margaret Pericak-Vance Division of Neurology, Box 2900 Duke University Medical Center Durham, NC 27710 Or: E-Mail: genclass@genemap.mc.duke.edu In any correspondence, please include a postal address From owner-chromosomes@net.bio.net Wed Dec 21 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!pipex!sunsite.doc.ic.ac.uk!charlie.lif.icnet.uk!mac034007.edin.icnet.uk!user From: h_gabra@icrf.icnet.uk (Hani Gabra) Newsgroups: bionet.genome.chromosomes Subject: type 16 rotor wanted Followup-To: bionet.genome.chromosomes Date: Thu, 22 Dec 1994 16:13:44 +0000 Organization: Imperial Cancer Research Fund Lines: 9 Message-ID: NNTP-Posting-Host: mac034007.edin.icnet.uk We need a type 16 or JA-14 Beckman Rotor on the cheap specifically for doing microcell fusion experiments. If anyone has one gathering dust in a corner of their lab which they don't need, we would be delighted to negotiate with them: money, part exchange etc. Necessity is the mother of invention. By the way, we're in Edinburgh.Please E-Mail me at h_gabra@icrf.icnet.uk or post a return message here. Regards Hani. From owner-chromosomes@net.bio.net Thu Dec 22 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!Germany.EU.net!ieunet!iol!a2-slip10.iol.ie!user From: casey@iol.ie ( Michael Casey) Newsgroups: bionet.genome.chromosomes Subject: Help: looking for a problem Date: 23 Dec 1994 10:30:30 GMT Organization: Ireland On-Line Lines: 11 Message-ID: NNTP-Posting-Host: a2-slip10.iol.ie Hi: I'm a computer sci postgrad, researching methods of solving certain problems (eg NP-Complete problems/Scheduling etc). I'm hoping to take different problems from various fields to complete my work. If anyone has any problems they suspect to be suitable please e-mail me at casey@iol.ie or kcasey@nova.ucd.ie (preferably the former over the christmas period). Many thanks. From owner-chromosomes@net.bio.net Mon Dec 26 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!nwnexus!calvino.alaska.net!carol.alaska.net!user From: carol@alaska.net (Carol) Newsgroups: bionet.genome.arabidopsis,bionet.genome.chrom22,bionet.genome.chromosomes,bionet.molbio.gene-linkage,bionet.molbio.genome-program Subject: NEW GENE SEQUENCER ANNOUNCED Date: Mon, 26 Dec 1994 20:27:40 -0800 Organization: Internet Alaska, Inc. Lines: 49 Message-ID: NNTP-Posting-Host: carol.alaska.net Xref: biosci bionet.genome.arabidopsis:2767 bionet.genome.chromosomes:381 bionet.molbio.gene-linkage:496 bionet.molbio.genome-program:1078 Introducing the new Helix Master 2000, now with polymerase cleaning action! The Helix Master 2000 is an exciting midrange genetic engine in our series new for 1995. We have an entire range of models from the the Transcriptionizer 250, suitable for the home and perfect for apartment renters, up through the more robust Homologous Recombinanizer 820, our first model to offer the Antisense RNA attachments! (This model requires three phase 220v for operation, and is not recommended for graduate student use). There are many genetic engines on the market. Why should you choose one from Acme? Simple. We know restriction enzymes! Many systems promise PCR amplification but don't always deliver. Either the thermal control is unstable or primers can anneal during early cycles yielding unsightly goo. Our systems don't suffer from this. Our engineers have combined the latest in Thermus Aquaticus Taq research with new bleaching agents so that your strands will be absolutely stable and whiter than white! What about the isolation of cloned genes? Most competing sytems still use the pMB9 and pBR322 cloning vectors developed by Thomas Edison in his groundbreaking research of the late 1890's. We have our own teams which developed plasmid vectors more advanced than any others on the market. Our new Polylinker 660, now with fizzing bubbles, is detectable by twice the markers of our competitors latest product. What about speed? Our sequencers are rated at over 1,500 base pairs per minute, and with the optional amino acid codon kit, you will be doing over 2,400 base pairs per minute! Each model comes fully equiped, ready to run with safety goggles as well as a short film about the dangers of drinking and deriving. Safety first! Please keep Eukaryotic genes out of the hands of children! Where are we heading? With new government regulations for steroid receptors slated for mid 1997 the entire membrane signal industry will be thrown into disarray. Our scientists are busy, even today, working with new signals and transcription factors such as Phosphorylation with a kinase rating over 6 points higher than California requirements to be in effect by June of 1997. Using our machines you'll be cloning armies of obediant slaves while the rest of the industry is tied up chasing expression plasmids around and getting no where. When you think genetic engines, think Acme genetic engines! "With Acme, your gene pool is generally safe." -- Andrew Carol "Could be worse. Could be raining." carol@alaska.net carol@ctis.af.mil 71350.3646@compuserve.com From owner-chromosomes@net.bio.net Wed Dec 28 22:00:00 1994 Path: biosci!MENDEL.LLNL.GOV!greg From: greg@MENDEL.LLNL.GOV Newsgroups: bionet.genome.chromosomes Subject: Re: pBluescript primers Date: 29 Dec 1994 12:02:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412292002.AA03966@mendel.llnl.gov> Reply-To: Greg Lennon NNTP-Posting-Host: net.bio.net The primer sequences which we found give somewhat more accurate sequence reads are, for the M13 Forward primer direction : aaacgacggccagtgag and for the M13 Reverse primer direction : caattaaccctcactaa I would be happy to hear of results (positive, negative, or neutral !) from anyone who compares these to other sequencing primers. Greg Lennon Human Genome Center Lawrence Livermore National Laboratory > From BIOSCI-REQUEST@net.bio.net Thu Dec 29 06:45:13 1994 > To: biochrom@net.bio.net > From: > Subject: pBluescript primers > > Dear Newsreaders , > > I am looking for either the referance or sequence of the > improaved Bluescript primers mentioned in the paper by J Perry and > G Lennon FASEB Journal 94 vol 8 no 7 pg A1362 . They are said to > prime moreaccuratly than the standard commercial primers thus > giving better reads . > I will make sure that the sequence is posted on the > newsgroup if anyone can supply it along with any follow up results > I have . > > Sincerely , > > David Cain > Email cain@icr.ac.uk > > From owner-chromosomes@net.bio.net Wed Dec 28 22:00:00 1994 Path: biosci!agate!sunsite.doc.ic.ac.uk!uknet!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: pBluescript primers Date: 29 Dec 1994 14:34:05 -0000 Lines: 15 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3duhct$ndk@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: pluto: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Dear Newsreaders , I am looking for either the referance or sequence of the improaved Bluescript primers mentioned in the paper by J Perry and G Lennon FASEB Journal 94 vol 8 no 7 pg A1362 . They are said to prime moreaccuratly than the standard commercial primers thus giving better reads . I will make sure that the sequence is posted on the newsgroup if anyone can supply it along with any follow up results I have . Sincerely , David Cain Email cain@icr.ac.uk From owner-chromosomes@net.bio.net Wed Dec 28 22:00:00 1994 Path: biosci!agate!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!usenet.ucs.indiana.edu!raven.bio.indiana.edu!user From: lwashing@sunflower.bio.indiana.edu (Lawrence Washington) Newsgroups: bionet.genome.chromosomes Subject: Re: NEW GENE SEQUENCER ANNOUNCED Date: 29 Dec 1994 20:08:35 GMT Organization: Indiana University Lines: 26 Message-ID: References: NNTP-Posting-Host: raven.bio.indiana.edu In article , carol@alaska.net (Carol) wrote: > When you think genetic engines, think Acme genetic engines! "With > Acme, your gene pool is generally safe." > > -- > Andrew Carol "Could be worse. Could be raining." > carol@alaska.net carol@ctis.af.mil 71350.3646@compuserve.com God BLESS you, Acme! You will be getting our purchase requisition for one Helix Master 2000 immediately. Our old Baserator Classique has been a real work horse (you know the one...coal fired, live steam polymerase turbines, cast iron extension chambers, manufactured back when Detroit still knew how to craft fine, if a bit garish, molecular machinery.) But when the genetic drift pressure valves on those things fail a man could loose a finger. So it is worth it to us to upgrade. -- Lawrence Washington Indiana University Institute for Molecular and Cellular Biology From owner-chromosomes@net.bio.net Fri Dec 30 22:00:00 1994 Path: biosci!ALLIANT.SNU.AC.KR!chunglee From: chunglee@ALLIANT.SNU.AC.KR Newsgroups: bionet.genome.chromosomes Subject: new user Date: 30 Dec 1994 20:40:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412310439.AA10026@alliant.snu.ac.kr> NNTP-Posting-Host: net.bio.net I would like to participate in this newsgroup