From owner-chromosomes@net.bio.net Wed Jan 04 22:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: BioTech NET Date: 5 Jan 1995 14:19:39 -0000 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3egv5r$eem@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: pluto: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Dear fellow newsreaders , Does anyone have any ideas as how to join the bulletin board BioTech NET which is often mentioned in Biotechniques ( eg vol 13 , no 1 1992 pg 46 ) . Thanks for your assistance and have a great new year . David Cain Email cain@icr.ac.uk From owner-chromosomes@net.bio.net Wed Jan 04 22:00:00 1995 Path: biosci!HELIX.MGH.HARVARD.EDU!HAINES From: HAINES@HELIX.MGH.HARVARD.EDU ("Jonathan L. Haines") Newsgroups: bionet.genome.chromosomes Subject: COURSE DEADLINE EXTENDED Date: 4 Jan 1995 06:27:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 164 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HLFZ7WPL0I8XGW9T@HELIX.MGH.HARVARD.EDU> ********************************* * COURSE ANNOUNCEMENT * ********************************* *************************************** * LAST CALL!! * * DEADLINE EXTENDED DUE TO HOLIDAY!! * *************************************** ---GENETIC ANALYSIS METHODS FOR MEDICAL RESEARCHERS--- Description: This intensive four day course centers on mapping human genetic diseases. The concentration is on the entire disease mapping process, including clinical classification, pedigree collection, molecular genetic analysis, statistical analysis, and gene characterization. The course emphasizes the global decision-making process, rather than details of specific techniques. A residential conference center setting promotes extensive interaction between the students and the internationally recognized faculty. Course goals: 1. To outline and instruct participants about the necessary steps and procedures used in ascertaining and collecting pedigree data and clinical and family history information. 2. To discuss in detail both the molecular and statistical methodologies for using the reference marker maps generated by the efforts of the Human Genome Initiative. Emphasis will be on working examples and the basic theory behind genetic mapping methodologies. 3. To educate participants on the general interpretation of linkage results for both simple and complex disorders. Discussions will include an overview of positional cloning strategies, refinement of the preliminary linkage data, investigation of power, examination of heterogeneity, physical mapping, cDNA analysis, exon amplification, etc. This course will not include any bench or "wet" laboratory experience. Co-organizers: Margaret A. Pericak-Vance, Ph.D., Department of Medicine, Department of Genetics, Duke University School of Medicine. She is a founding fellow of the American College of Medical Genetics (ABMS) and a board-certified Ph.D. medical geneticist with fourteen years experience in genetic counseling. Dr. Pericak-Vance is a genetic epidemiologist whose research has focused on mapping the human genome with emphasis on the mapping of genetic disorders, with more recent extensions into complex genetic disease. She has concentrated on neurogenetic diseases, actively mapping such disorders as familial amyotrophic lateral sclerosis (Lou Gehrig disease), tuberous sclerosis, and late onset Alzheimer disease (AD). She is a member of the Mammalian Genetics Study Section at NIH. Jonathan L. Haines, Ph.D., Department of Neurology, Massachusetts General Hospital, Harvard Medical School. Dr. Haines is a genetic epidemiologist whose research has focused on both human disease and general (reference) chromosome mapping. Dr. Haines has concentrated on neurogenetic diseases, including the mapping of familial amyotrophic lateral sclerosis, familial Alzheimer disease, Huntington disease, tuberous sclerosis, neurofi- bromatosis, and multiple sclerosis. Dr. Haines is presently an editor of chromosome 9 for the Genome Data Base (GDB). He is an associate editor of Genomics and an editor of Current Protocols in Human Genetics. Instructors: Arthur S. Aylsworth, M.D., Division of Genetics and Metabolism, Department of Pediatrics, The University of North Carolina at Chapel Hill. Dr. Aylsworth is a board-certified pediatrician and clinical geneticist. As a physician-scientist, Dr. Aylsworth plays primary roles in family ascertainment and phenotypic delineation. He is actively involved in studies on neurofibromatosis and neural tube defects. David Goldgar, Ph.D., Genetic Epidemiology, Department of Medical Informatics, University of Utah. Dr. Goldgar is a statistical geneticist with extensive experience in both the theoretical and practical aspects of mapping human quantitative and complex traits. He has concentrated on the genetic epidemiology of common cancers, focusing on the genetic mapping of breast and ovarian cancer. He is actively involved in several large pedigree ascertainment and collection studies. Deborah A. Meyers, Ph.D., Department of Medicine, The Johns Hopkins University. Dr. Meyers is a statistical geneticist who has concentrated her research on the genetic basis of complex disorders including allergy and psychiatric diseases such as schizophrenia and Alzheimer disease. She has extensive didactic experience in her teaching role at the short course in Medical and Experimental and Mammalian Genetics, The Jackson Laboratory, Bar Harbor, Maine. Jeffrey Murray, M.D., Department of Pediatrics, University of Iowa. Dr. Murray is a molecular biologist and physician scientist with extensive experience in both general chromosome and disease gene mapping. Dr. Murray is a board-certified pediatrician and clinical geneticist. His research interests include genetic studies in cleft-lip and palate. He also directs a national effort to generate a high resolution genetic map of the entire genome. Dr. Murray is a GDB Editor for chromosome 4 and a former member of the Mammalian Genetics Study Section at NIH. Marcy C. Speer, M.S., Ph.D., Department of Medicine, Duke University Medical School. Dr. Speer is a board-certified genetic counselor with eleven years of experience. She is also a genetic epidemiologist whose research interest is in mapping human genetic diseases, including the muscular dystrophies and neural tube defects. She is also involved in studies of unusual genetic phenomena such as anticipation and imprinting. Participation: Participation in the course will be dependent on completion of an application form that describes the applicant's background and research interests and will be limited to 36 students. All participants will need to show evidence of a postgraduate genetics course or its equivalent. Participants must provide a brief statement describing their research interests, their reason for taking the course, and their long-term objectives in relation to the course curriculum. This information will be used to select a highly motivated participant group. Minority and women applicants are specifically encouraged to apply. A limited number of scholarships are available for registered students or fellows. Scholarship selection will be based on the strength of the individual applications. Location: The course will be held at the R. David Thomas Center located on the campus of Duke University, Durham, NC. The Thomas Center is a new facility which establishes a climate of hospitality and an atmosphere conductive to learning and to the exchange of ideas. Both students and faculty will be housed at the R. David Thomas Center. Date: March 25-29, 1995 Extended deadline for completed application: January 9, 1995 For information and application forms contact: Write: Genetic Methods Course c/o Dr Margaret Pericak-Vance Division of Neurology, Box 2900 Duke University Medical Center Durham, NC 27710 Or: E-Mail: genclass@genemap.mc.duke.edu In any correspondence, please include a postal address From owner-chromosomes@net.bio.net Wed Jan 04 22:00:00 1995 Newsgroups: bionet.drosophila,bionet.general,bionet.genome.arabidopsis,bionet.genome.chrom22,bionet.genome.chromosomes,bionet.immunology,bionet.info-theory,bionet.jobs,bionet.journals.note,bionet.metabolic-reg,bionet.molbio.ageing,bionet.molbio.bio-matrix,bionet.molbio.embldatabank,bionet.molbio.evolution,bionet.molbio.gdb,bionet.molbio.genbank,bionet.molbio.gene-linkage,bionet.molbio.genome-program,bionet.molbio.hiv Path: biosci!bloom-beacon.mit.edu!gatech!newsfeed.pitt.edu!uunet!xnet!quake.xnet.com!research From: crta@xnet.com (Norman Fraley) Subject: New Research & Testing Association Formed Message-ID: Sender: news@amiserv.chi.il.us Nntp-Posting-Host: research.crta.org Organization: Contract Research & Testing Association X-Newsreader: News Xpress Version 1.0 Beta #2 Date: Thu, 5 Jan 1995 20:52:00 GMT Lines: 66 Xref: biosci bionet.drosophila:777 bionet.general:12714 bionet.genome.arabidopsis:2789 bionet.genome.chromosomes:390 bionet.immunology:2755 bionet.info-theory:3047 bionet.jobs:6983 bionet.journals.note:371 bionet.metabolic-reg:390 bionet.molbio.ageing:1085 bionet.molbio.bio-matrix:526 bionet.molbio.embldatabank:419 bionet.molbio.evolution:2265 bionet.molbio.gdb:273 bionet.molbio.genbank:1873 bionet.molbio.gene-linkage:506 bionet.molbio.genome-program:1101 bionet.molbio.hiv:814 As the primary resource of research information, the Internet was the primary choice for making all concerned individuals aware of the formation of the Contract Research & Testing Association. CRTA is an International Association designed to serve the needs of contract research, product and process development organizations and consultants throughout the world. Contract research organizations have specific public, governmental, and industry perception and promotion needs which are not addressed by existing scientific industry associations. CRTA operates as a non-profit, tax-exempt, corporation eligible for scientific research and public awareness charitable organization contributions as provided for in the IRC 501(c)(3) provisions. Being a scientific research and public awareness related organization, CRTA exists to benefit its members by providing: 1) An organization devoted to the promotion of Contract Research. 2) A unified voice on matters of common interest or concern. 3) Point of contact for media relations relative to contract research. 4) Business opportunity referrals as a research clearinghouse. 5) Professional networking opportunities for its members. 6) Periodic publishing of information beneficial to the membership. 7) Periodic dissemination of applicable research results to the public. 8) Governmental representation on issues affecting CRO's. 9) Public promotion of the strengths of its membership. 10) A directory of Contract Research Organizations and Consultants. CRTA will provide: 1) A forum for the exchange of information. 2) Formal recognition to the CRO's role in business. 3) Standards for the professionals so engaged. 4) Representation the profession in matters of common interest. 5) The development of techniques and methods to improve the practice and management of CROs. CRTA will also offer: 1) A monthly news publication. 2) Annual meetings 3) Active promotional media publicity programs. 4) A professional placement service 5) A Contract Research Service Directory. 6) Media topics and contacts directory If you have an interest in joining the Contract Research & Testing Association, please E-mail your reply to crta@xnet.com. Please include: 1) The word "membership" in your RE: or header information, 2) Your interest in the association / your area of work, 3) Your dues payment preference (check, money order, credit card, company check, wire xfer, etc.) DO NOT INCLUDE ANY CREDIT CARD INFORMATION! Only your preference for the manner of payment. 4) Most importantly, your email address, and additional contact information if you desire. We will then e-mail membership information and ALL FURTHER INFORMATION directly to you at your email location. Thank you for taking the time to read this announcement. If membership in this program this does not appeal to you, thank you for your patience and understanding. Sincerely, Membership Department Contract Research & Testing Association Best Regards, Norman Fraley CRTA@xnet.com Executive Director BBS:708-515-0494 Contract Research & Testing Association From owner-chromosomes@net.bio.net Wed Jan 04 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!gatech!newsfeed.pitt.edu!uunet!fdn.fr!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!rzusuntk.unizh.ch!lucas From: lucas@molbio2.unizh.ch (Lucas Leuzinger) Subject: Warblefly, Botfly or Dermatobia sp. ???? Message-ID: <1995Jan5.184054.24054@rzu-news.unizh.ch> Sender: newsadm@rzu-news.unizh.ch (CNEWS ADMINISTRATION) Organization: University of Zurich, Switzerland X-Newsreader: TIN [version 1.2 PL2] Date: Thu, 5 Jan 1995 18:40:54 GMT Lines: 18 Hi ! I need for my diploma-thesis some or any information about the BOTFLY or the WARBLEFLY, Dermatobia hominis (Oestridae), that live in warm Central- and Southamerica ... Do you know any specialist or person working on this subject, or parasitic flies in general ???, any source of information ??? I'm very grateful for any kind of help !!! Please write me : Lucas Leuzinger lucas@molbio2.unizh.ch Thanks a lot! From owner-chromosomes@net.bio.net Wed Jan 04 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!uhog.mit.edu!nntp.club.cc.cmu.edu!newsfeed.pitt.edu!uunet!fonorola!inforamp.net!woody16.inforamp.net!intermed From: intermed@inforamp.net (Ian Clarke) Newsgroups: bionet.genome.chromosomes Subject: Attention Canadian Researchers Date: Thu, 5 Jan 1995 12:51:30 Organization: InfoRamp Inc. Lines: 19 Message-ID: NNTP-Posting-Host: woody16.inforamp.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Inter Medico is the Canadian distributor for many of the world's leading molecular biology companies. Inter Medico represents Genzyme(cytokines, chemokines, cell adhesion molecules), Novagen (pET bacterial expression systems, mRNA purification systems), Amresco (fine chemicals, thermostable DNA polymerases, nucleic acid isolation kits), SLT (ELISA readers, fluorometers, microplate washers), BioDesign (a huge selection of biologically important antibodies), and The Binding Site ( clinically-relevant antibody-based diagnostic systems). As part of Inter Medico's continuing dedication to the Canadian research community, we have established a user-group to share the latest information about molecular biology products designed to save you time and money. There are valuable discounts available only to subscribers. There is no charge to join. If you are interested in joining, please send an e-mail message to Majordomo@inforamp.net. In the body of the letter, type Subscribe Research . Join today, and start enjoying the benefits immediately. From owner-chromosomes@net.bio.net Thu Jan 05 22:00:00 1995 Path: biosci!biosci!not-for-mail From: lstein@genome.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.genome.chromosomes,bionet.announce Subject: SSLP GENETIC MAP OF THE RAT Date: 6 Jan 1995 15:18:09 -0800 Organization: Massachusetts General Hospital/MIT Genome Center Lines: 88 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <3eka3b$4mj@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: net.bio.net Keywords: rat rattus norvegicus simple sequence repeat polymorphisms Xref: biosci bionet.genome.chromosomes:391 bionet.announce:1668 MASSACHUSETTS GENERAL HOSPITAL/MIT CENTER FOR GENOME RESEARCH GENETIC MAP OF THE RAT RELEASE 1 JANUARY 1995 INTRODUCTION: This is to announce the January 1995 release of the Massachusetts General Hospital/Harvard Medical School and Whitehead Institute/MIT Center for Genome Research rat genetic map. This map consists of 431 polymorphic rat microsatellite repeats mapped on a SHRxBN F2 intercross using PCR probes. The average spacing between markers on these maps is approximately 3.7 cM. OBTAINING THE DATA: The data can be obtained as a series of flat files by anonymous ftp to ftp-genome.wi.mit.edu. Log in as "anonymous" and give your e-mail address as your password. The data files can be found in /distribution/rat_sslp_releases/janary95. Alternatively, the data can be found on a World Wide Web site maintained by the MIT Center for Genome Research. Point your browser at http://www-genome.wi.mit.edu/ and follow the links to Genome Center Data/Rat Genetic Mapping Project. CONTENTS OF THIS DIRECTORY: The contents of this directory are as follows: marker_data.sea.hqx A Macintosh Excel 4.0 format file containing data on each of the markers, including the sequence of forward and reverse primers, the genotype of the markers on the cross, and the sizes of the product produced on a number of standard inbred strains. marker_data.txt As above in a tab-delimited ASCII text file. chromosome_pictures/ A directory containing Macintosh picture files of the maps, one per chromosome. CITING THIS DATA: References to this data in publications should be cited by listing the following source: 1. Jacob, H.J., Brown, D.M., Bunker, R.K., Daly, M.J., Dzau, V.J., Goodman, A., Koike, G., Kren, V., Kurtz, T., Lernmark, A., Levan, G., Mao, Y-P., Pettersson, A., Pravenec, M., Simon, J.S., Szpirer, C., Szporer, J., Trolliet, M.R., Winer, E.S. and Lander, E.S., 1995. A genetic linkage map of the laboratory rat, Rattus norvegicus, Nature Genetics 9, 63-69. ASSAY CONDITIONS: Assay conditions and other experimental details are given in Jacob, H.J., et al., 1995. Nature Genetics 9, 63-69. Briefly, the PCR protocol used radiolabeled primers in a 25 cycle PCR (1 '92 degrees, 2' 55 degrees, 1.5' 72 degrees) on 20 ng of genomic DNA. PURCHASING PRIMER PAIRS: All the primers listed in this release are available from Research Genetics, Inc.,2130 Memorial Parkway SW, Huntsville, AL 35801, 800-533-4363 (phone), 800-336-9014 (FAX), under a community discount arrangement set up by the Massachusetts General Hospital/Harvard Medical School and the Whitehead Institute/MIT Center for Genome Research. (Note: MGH and WI/MIT CGR have placed the markers in the public domain. The center and its personnel receive no financial benefit from the sale of primers.) FURTHER QUESTIONS: Please direct questions and inquiries to Donna Brown, telephone (617) 726-4347, d_brown@helix.mgh.harvard.edu. Donna Brown Massachusetts General Hospital -- ======================================================================== Lincoln D. Stein Whitehead Institute/MIT Genome Center lstein@genome.wi.mit.edu Cambridge, MA 02142 ======================================================================== From owner-chromosomes@net.bio.net Mon Jan 09 22:00:00 1995 Path: biosci!CROP.UOGUELPH.CA!SPLUHAR From: SPLUHAR@CROP.UOGUELPH.CA Newsgroups: bionet.genome.chromosomes Subject: Re: DNA cloning and animal cloning... Date: 10 Jan 1995 08:58:11 -0800 Organization: Crop Science, The Univ. of Guelph Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <1A01ACC3483E@csnet.nw.uoguelph.ca> NNTP-Posting-Host: net.bio.net > I am a relative amateur when it comes to the field of Biology, but I > have become somewhat interested in the genetic side. After having some > courses in Biology, I am aware that it is possible to reproduce DNA > material. > > However, I was wondering if someone could enlighten me on the progress > of the cloning of animals, specifically median mammals such as dogs. At present they are unable to clone mammals. There were some experiments involving frog's cell nuclei from a frog's intestinal cells. The nuclei were taken from the intestinal cells and inserted into frog egg cells that had had their nuclei destroyed. 1 percent of the eggs produced live frogs which were genetically identical. I think similar experiments were also done on other amphibians. So far as I know this has not been done successfully with mammals. You can produce genetically identical animals by taking a Zygote when it is in the 4 cell stage and separating the cells from each other and transplanting them into different parents. Each of the cells will give rise to a whole new animal and each of the 4 animals produced will be identical to the other 3. I believe this procedure is regularly done on cows. I don't think it has been done on dogs though. Sorry about the second post but my last post seemed like it might be a little hard to read so I reworded it a bit. ********************************************************************* * Stephen A. Pluhar * * SPLUHAR@CROP.UOGUELPH.CA Dept. Crop Science U. of Guelph * * Phone: 519-824-4120 Ext. 4865 Guelph, Ontario, Canada. N1G 2W1 * * Fax 519-763-8933 * *-------------------------------------------------------------------* * Building better plants for a better tomorrow. * ********************************************************************* * Have a nice day :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) ;)* ********************************************************************* From owner-chromosomes@net.bio.net Mon Jan 09 22:00:00 1995 Path: biosci!CROP.UOGUELPH.CA!SPLUHAR From: SPLUHAR@CROP.UOGUELPH.CA Newsgroups: bionet.genome.chromosomes Subject: Re: DNA cloning and animal cloning... Date: 10 Jan 1995 07:59:26 -0800 Organization: Crop Science, The Univ. of Guelph Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <1A00B12142F8@csnet.nw.uoguelph.ca> NNTP-Posting-Host: net.bio.net > I am a relative amateur when it comes to the field of Biology, but I > have become somewhat interested in the genetic side. After having some > courses in Biology, I am aware that it is possible to reproduce DNA > material. > > However, I was wondering if someone could enlighten me on the progress > of the cloning of animals, specifically median mammals such as dogs. At present they are unable to clone mammals. There were some experiments involving frogs nuclei from a frog's intestinal cells were taken and inserted into frog egg cells that had had their nuclei destroyed. 1 percent of the eggs produced live frogs which were genetically identical. So far as I know this has not been done successfully with mammals. You can produce genetically identical animals by taking a Zygote when it is in the 4 cell stage and separating the cells from each other and transplanting them into different parents. Each of the cells will give rise to a whole new animal and each of the 4 animals produced will be identical to the other 3. I believe this procedure is regularly done on cows. I don't think it has been done on dogs though. > > I would appreciate any information regarding the above topics via e-mail. > Thank you very much for your attention and consideration! > > -Mike Woycheck > I hope this information helps. ********************************************************************* * Stephen A. Pluhar * * SPLUHAR@CROP.UOGUELPH.CA Dept. Crop Science U. of Guelph * * Phone: 519-824-4120 Ext. 4865 Guelph, Ontario, Canada. N1G 2W1 * * Fax 519-763-8933 * *-------------------------------------------------------------------* * Building better plants for a better tomorrow. * ********************************************************************* * Have a nice day :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) :) ;)* ********************************************************************* From owner-chromosomes@net.bio.net Mon Jan 09 22:00:00 1995 Path: biosci!rutgers!uwm.edu!spool.mu.edu!howland.reston.ans.net!news.cac.psu.edu!tsppp17.cac.psu.edu!maw153 From: maw153@psu.edu (Michael Woycheck) Newsgroups: bionet.genome.chromosomes Subject: DNA cloning and animal cloning... Date: Tue, 10 Jan 1995 00:13:55 GMT Organization: CAC Lines: 12 Message-ID: NNTP-Posting-Host: tsppp17.cac.psu.edu Keywords: DNA cloning animal dog X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] I am a relative amateur when it comes to the field of Biology, but I have become somewhat interested in the genetic side. After having some courses in Biology, I am aware that it is possible to reproduce DNA material. However, I was wondering if someone could enlighten me on the progress of the cloning of animals, specifically median mammals such as dogs. I would appreciate any information regarding the above topics via e-mail. Thank you very much for your attention and consideration! -Mike Woycheck From owner-chromosomes@net.bio.net Mon Jan 09 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!agate!news.mindlink.net!news From: Peter_MacDougall@mindlink.bc.ca (Peter MacDougall) Newsgroups: bionet.genome.chromosomes Subject: Re: DNA cloning and animal cloning... Date: 10 Jan 1995 17:25:24 GMT Organization: MIND LINK! Communications Corp. Lines: 17 Message-ID: <3eufu4$g49@deep.rsoft.bc.ca> References: Reply-To: Peter_MacDougall@mindlink.bc.ca (Peter MacDougall) NNTP-Posting-Host: line08.nwm.mindlink.net X-Newsreader: IBM NewsReader/2 v1.09 In , maw153@psu.edu (Michael Woycheck) writes: >I am a relative amateur when it comes to the field of Biology, but I >have become somewhat interested in the genetic side. After having some >courses in Biology, I am aware that it is possible to reproduce DNA >material. > >However, I was wondering if someone could enlighten me on the progress >of the cloning of animals, specifically median mammals such as dogs. Unlike Stephen says, they have successfully cloned pigs and cows. Look in back issues of US News for reports. Peter.MacDougall@mindlink.bc.ca PEM@unixg.ubc.ca Reality always exceeds your expectations From owner-chromosomes@net.bio.net Tue Jan 10 22:00:00 1995 Path: biosci!rutgers!gatech!newsfeed.pitt.edu!rar From: rar+@pitt.edu (Richard A Rubin) Newsgroups: bionet.software,bionet.genome.chromosomes Subject: Wanted: Hilditch methods for chromosome analysis Date: 11 Jan 1995 00:55:47 GMT Organization: University of Pittsburgh Lines: 4 Message-ID: <3evaaj$gf6@usenet.srv.cis.pitt.edu> NNTP-Posting-Host: unixd4.cis.pitt.edu Xref: biosci bionet.software:10602 bionet.genome.chromosomes:396 Hilditch published some methods for automated chromosome analysis using skeletonization, some 20+ years ago. I'm looking for algorithms or code for these methods (beyond the skeletonization itself). Is this available anywhere? From owner-chromosomes@net.bio.net Wed Jan 11 22:00:00 1995 Path: biosci!MAILGATE.CSMC.EDU!rschreck From: rschreck@MAILGATE.CSMC.EDU ("RhonaS") Newsgroups: bionet.genome.chromosomes Subject: chromosome news group Date: 11 Jan 1995 09:01:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: please send info about newsgroup and how to participate. From owner-chromosomes@net.bio.net Wed Jan 11 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!sol.ctr.columbia.edu!newsxfer.itd.umich.edu!agate!spool.mu.edu!olivea!uunet!newsflash.concordia.ca!canopus.cc.umanitoba.ca!umglodj0 From: umglodj0@cc.umanitoba.ca (Armansa Glodjo) Newsgroups: bionet.genome.chromosomes Subject: Request information on Y-linked genes Date: 12 Jan 1995 14:02:37 GMT Organization: University of Manitoba, Winnipeg, Manitoba, Canada Lines: 13 Distribution: world Message-ID: <3f3cpt$1ud@canopus.cc.umanitoba.ca> NNTP-Posting-Host: pollux.cc.umanitoba.ca I was wondering if anyone could provide a list of Y-linked genes and their developmental influences, or provide me with a site where I could find the information myself. Thanks in advance, Armansa Glodjo -- ---------------------------------------------------------------- Armansa Glodjo "Trust No One--Deny Everything" Honors Genetics III, University of Manitoba, Canada ---------------------------------------------------------------- From owner-chromosomes@net.bio.net Wed Jan 11 22:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!Germany.EU.net!gsf.de!cony.gsf.de!baumgart From: baumgart@cony.gsf.de (Adolf Baumgartner) Newsgroups: bionet.genome.chromosomes Subject: human Y- and X-chromosome probes ? Date: 12 Jan 1995 10:09:58 GMT Organization: GSF Forschungszentrum fuer Umwelt und Gesundheit Lines: 20 Distribution: world Message-ID: <3f2v5m$spv@cony.gsf.de> Reply-To: somebody@gsf.de NNTP-Posting-Host: cony.gsf.de Keywords: Y-chromosome, X-chromosome, human specific probe I'm interested in human Y- and X-chromosome specific probes for fluorescence in situ hybridization. I want to label this probes with various fluorescent dyes, so I would be very pleased, if I could get any clones with these probes. Any help is requested. Thank you in advance, Adi. ------------------------------------- Email: baumgart@gsf.de Address: Adolf Baumgartner GSF - Forschungszentrum Institut für Sägetiergenetik 85758 Oberschleissheim Germany Phone : +49-89-3187-4628 FAX : +49-89-3187-2210 From owner-chromosomes@net.bio.net Wed Jan 11 22:00:00 1995 Path: biosci!newshost.lanl.gov!news.ttu.edu!seas.smu.edu!convex!insosf1.infonet.net!solaris.cc.vt.edu!news.alpha.net!uwm.edu!psuvax1!news.ecn.bgu.edu!newspump.wustl.edu!fas-news.harvard.edu!lipid!robison From: robison@lipid.harvard.edu (Keith Robison) Newsgroups: bionet.genome.chromosomes Subject: Re: Request information on Y-linked genes Date: 12 Jan 1995 15:34:31 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 23 Distribution: world Message-ID: <3f3i67$ng1@decaxp.harvard.edu> References: <3f3cpt$1ud@canopus.cc.umanitoba.ca> NNTP-Posting-Host: lipid.harvard.edu X-Newsreader: TIN [version 1.2 PL2] Armansa Glodjo (umglodj0@cc.umanitoba.ca) wrote: : I was wondering if anyone could provide a list of : Y-linked genes and their developmental influences, or : provide me with a site where I could find the information myself. Try the Genome Database using Mosaic or Netscape http://gdbwww.gdb.org/ Their forms interface supports just this sort of query, and I think you will have no trouble finding descriptions of all the known Y-linked genes (it ain't a big list!) Keith Robison Harvard University Department of Cellular and Developmental Biology Department of Genetics / HHMI robison@mito.harvard.edu From owner-chromosomes@net.bio.net Thu Jan 12 22:00:00 1995 Path: biosci!ATGENOME.BIO.UPENN.EDU!jecker From: jecker@ATGENOME.BIO.UPENN.EDU (Joe Ecker) Newsgroups: bionet.genome.chromosomes Subject: Instruments for large-scale PCR Date: 12 Jan 1995 09:58:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <9501121752.AA03690@atgenome.bio.upenn.edu> Dear Colleagues, We are in need of a reliable system for large-scale PCR (>1000 samples run). We are currently testing the Intelligent Automation Thermocycler- TC1600 (16 x 192 format). I would appreciate any advice from users of this instrument as to how they like it. Our primary use of the machine is for STS content mapping of YAC/P1 libraries and SSLP typing on recombinant inbred populations. However, I would be very interested if anyone has experience using the TC1600 for cycle sequencing. I would also be interested in hearing from anyone using a robotic cycler for the above tasks. I've seen a recent review of the Sanger Center conference on automation that describes several machines. However, I'm looking for an instrument that is currently available commercially. Thanks in advance, Joe Ecker ----- End Included Message ----- From owner-chromosomes@net.bio.net Thu Jan 12 22:00:00 1995 Path: biosci!WATSON.WUSTL.EDU!rwilson From: rwilson@WATSON.WUSTL.EDU (Rick Wilson) Newsgroups: bionet.genome.chromosomes Subject: CSH Genome Sequencing Course Date: 13 Jan 1995 14:00:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 180 Sender: daemon@net.bio.net Distribution: world Message-ID: <9501132201.AA01898@watson.wustl.edu> NNTP-Posting-Host: net.bio.net Cold Spring Harbor Laboratory ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 Correspondence should be addressed to: """"""""""""""""""""""""""""""""""""""""""""" Cold Spring Harbor Laboratory The Meetings Office 1 Bungtown Road, P.O. Box 100 Cold Spring Harbor New York 11724-2213 Phone: (meetings) 516 367 8346 (courses) 516 367 8345 Fax: 516 367 8845 E-mail: meetings@cshl.org World wide web site at http://www.cshl.org/ """"""""""""""""""""""""""""""""""""""""""""" CONTENTS 1) Introduction 2) Full description of course (dates, instructors, description, application deadline) 3) Short list of meetings and courses 4) Information for course applicants 5) 1995 course price 6) Sponsors of the 1994 Courses Program 7) Getting to Cold Spring Harbor Laboratory 8) General information about Cold Spring Harbor Laboratory Application forms and further information can be obtained from the above address. 1) Introduction Cold Spring Harbor Laboratory is a non-profit independent research and educational institution chartered by the University of the State of New York. The Laboratory employs 550 people, including a research staff of almost 200 Ph.D. scientists and 50 graduate students. Cold Spring Harbor Laboratory's primary goal is to carry out first-rate scientific research. However, the Laboratory pursues other goals which we believe are vital to the well-being of science and scientists: scientific communication; training scientists; public education; and publishing. The Laboratory is located thirty-five miles east of New York City on the scenic north shore of Long Island. Each year, over 5,000 scientists worldwide attend one or several of the more than 15 scientific meetings held at Cold Spring Harbor Laboratory in the award- winning Grace Auditorium. The flagship of this program is the annual Symposium on Quantitative Biology, one of the most prestigious meetings in molecular biology. Professional meetings are an integral part of a scientist's job; they are a primary means by which scientists stay current on the latest results and techniques in their field. Complementing our meetings program is our annual program of 25 high-level courses. Directed primarily at post-doctoral level scientists, these intensive courses allow practicing researchers to immerse themselves in new techniques and ideas that they can apply immediately to their own research. Many of these courses are laboratory courses held in our Beckman Neuroscience Center, which was completed in 1991. For more than 50 years, courses at Cold Spring Harbor have trained the rising stars in emerging disciplines and shaped the direction of research, both at Cold Spring Harbor and elsewhere. 2) Full Description of Course Application Deadline January 31, 1995 ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 Ellson Y. Chen, Perkin Elmer Corporation Richard Gibbs, Baylor College of Medicine W. Richard McCombie, Cold Spring Harbor Laboratory Richard K. Wilson, Washington University Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequencers to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution. Students will work in groups to sequence a large region of DNA and through this process be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA! sequencing. 3) Short list of 1995 meetings and courses 1995 Meetings (in chronological order) Molecular and Behavioral Biology of Aplysia and Related Molluscs The Cytoskeleton & Cell Function Tyrosine Phosphorylation & Cell Signalling Genome Mapping & Sequencing RNA Processing Retroviruses Symposium: Protein Kinesis - The Dynamics of Protein Trafficking & Stability Yeast Cell Biology Molecular Genetics of Bacteria & Phages Cancer Cells: Mechanisms of Eukaryotic Transcription Eukaryotic DNA Replication Molecular Approaches to the Control of Infectious Diseases Programmed Cell Death Plant Signalling in Development Neurobiology of Drosophila 1995 Courses (in alphabetical order) Advanced Bacterial Genetics Advanced Genome Sequence Analysis Advanced In Situ Hybridization & Immunocytochemistry Advanced Molecular Cloning & Expression of Eukaryotic Genes Arabidopsis Molecular Genetics Cloning and Analysis of Large DNA Molecules Computational Genomics Early Development of Xenopus Laevis Macromolecular Crystallography Molecular Embryology of the Mouse Molecular Markers for Plant Breeding & Plant Genetics Phage Display of Combinatorial Antibodies Protein Purification & Characterization YACs in Structural and Biological Genome Analysis Yeast Genetics Courses in Neurobiology Brain Mapping Developmental Neurobiology Imaging Structure and Function in the Nervous System Molecular Approaches to Ion Channel and Function Molecular Cloning of Neural Genes Neurobiology of Drosophila Neurobiology of Human Neurological Disease: Mechanisms of Human Neurodegeneration Structure, Function & Development of the Visual System The Biology of Memory: From Molecules to Behavior 4) Information for Course Applicants Registration: Mail the application form plus 3 additional copies of application and accompanying material to the Course Registrar on or before the course deadline. If applying for more than one course, a separate application form plus 3 additional copies must be submitted for each course. Please select one course per session. Selection Process: Each year, the instructors are confronted with the difficult task of selecting the students for our courses from a large number of well-qualified applicants. To facilitate the application process, students are asked to provide information documenting their request for a place in the course. First and foremost, applicants should give their reasons for wanting to take the course. As part of this, you should briefly outline your career. It is highly recommended that you supplement your application with one or more letters of recommendation. Applicants should remember that selection is based upon the degree to which they would benefit from this training opportunity; this in turn will be judged from the information given by the applicants. Applicants will be notified by mail whether or not they have been accepted to attend. The decisions often take several weeks after the application deadline to be finalized and announced. Stipends: Scholarship support is available to all qualified applicants based on need. Any candidate requesting financial assistance MUST request it when submitting the application. Attach a separate letter stating the amount requested and give a full justification for the required support. The amount awarded will be based upon the availability of funding. Housing: All students must live in the Laboratory dormitories and take meals in the dining room. Most of the rooms are multiple occupancy and/or shared bath. Please be sure to indicate gender on the application form. Due to space limitations, families cannot be housed on grounds; there are no exceptions to this policy! Course Application Checklist: Mail original plus 3 copies of the following: ** Original application for each course applied for. ** Letter and/or curriculum vitae outlining your career including reasons why taking the course would benefit you ** Letter(s) of recommendation **(Stipend request) 5) Prices for 1995 Courses: Length of Course Cost * 6 Days $1,155.00 1 Week $1,215.00 10 Days $1,460.00 2 Weeks $1,720.00 <= COST OF THIS COURSE 3 Weeks $2,180.00 * Cost includes tuition, food and housing 6) Sponsors of the 1994 Courses Program (not specifically for this course) The Howard Hughes Medical Institute ** The Esther A. & Joseph Klingenstein Fund, Inc. ** United States Department of Agriculture ** United States Department of Energy ** National Cancer Institute ** National Center of Human Genome Research ** National Institute of General Medical Sciences ** National Institute of Mental Health ** National Institutes of Health ** National Science Foundation 7) Getting to Cold Spring Harbor Laboratory ** New York Airports: J.F. Kennedy, La Guardia and L.I. MacArthur Airports are most convenient to the Laboratory (allow 1 1/2 - 2 hours travel time each way). Cold Spring Harbor Laboratory does not coordinate travel arrangements from local airports. ** Limousines: Prices for JFK and LaGuardia only! Syosset Limousine - 516-364-9681, reservation required. Private Car (1 - 9 people). Cost = $65.00 for 1 person, $33.00 pp. for 2, $25.00 pp. for 3 or more. Classic Limousine - 516-567-5100 (or 1-800-666-4949 outside New York). Shared Ride Service (possible stops at other terminals/destinations). Use courtesy phone at airport. No reservation required. Cost = $33.00 per person. Other transportation options are available at the airports. Do not use taxicabs for transportation from the airport to CSHL. ** Railroad: Long Island Railroad is located in Penn Station. Take the PT JEFFERSON BRANCH train to SYOSSET. Note: Possible transfer at Jamaica Station. Taxis usually meet arriving trains at Syosset station ticket office. Syosset Taxi 921-2141; $6.50 for 1 person, $4.00 each additional person. ** Automobile: From the Long Island Expressway (Rte 495), Exit 41 North (Route 106-107). On 106-107, bear right at fork (Route 106 - Oyster Bay). Travel 3.5 miles on Rte 106, make a right turn (East) onto Route 25A (town of East Norwich). Travel 4 miles. The lab entrance is on the left. (A gold Victorian house marks the entrance). ** New York - Connecticut Ferry Services: Port Jefferson - Bridgeport (516-473-0286) Orient Point - New London (516-323-2743) 8) General Information about services at Cold Spring Harbor Laboratory ** Registration: Please register at Grace Auditorium upon your arrival at the laboratory. Meetings begin at 7:30 in the evening. Dinner is served from 5:30 to 7:30 prior to meetings. Please call ahead if you plan to arrive at the lab after midnight. ** Parking: All vehicles MUST receive a Temporary Parking Permit from The Meetings Office. Any vehicle on grounds without such a permit will be subject to booting or towing. ** Phones / Faxes: Participants can receive faxes at the Meetings Office (516) 367-8845. Payphones and house phones are located throughout the campus. Bookstore: The Bookstore is located in the lower level of Grace Auditorium. Store hours are posted. ** Computer Facilities: Lower level of Grace Auditorium, equipped with PC, Mac & laser printer. To access your e-mail, you must know the name of your home server. """"""""""""""""""""""""""""""""""""""""""""" Correspondence should be addressed to: Cold Spring Harbor Laboratory The Meetings Office 1 Bungtown Road, P.O. Box 100 Cold Spring Harbor New York 11724-2213 Phone: (meetings) 516 367 8346 (courses) 516 367 8345 Fax: 516 367 8845 E-mail: meetings@cshl.org World wide web site at http://www.cshl.org/ """"""""""""""""""""""""""""""""""""""""""""" From owner-chromosomes@net.bio.net Fri Jan 13 22:00:00 1995 Path: biosci!biosci!not-for-mail From: robison@golgi.harvard.edu (Keith Robison) Newsgroups: bionet.software,bionet.announce,bionet.molbio.genome-program,bionet.genome.chromosomes Subject: Announce: The 100Kb Club Date: 13 Jan 1995 16:30:51 -0800 Organization: Harvard University, Cambridge, Massachusetts Lines: 16 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <3f51v9$2ft@decaxp.harvard.edu> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.software:10635 bionet.announce:1686 bionet.molbio.genome-program:1114 bionet.genome.chromosomes:404 Since it is a subject that pops up frequently, I have created an HTML listing of all publically-available DNA sequences 100Kb or longer. It is available at the URL http://golgi.harvard.edu/100kb/ Please let me know of any corrections or updates to this list. In particular, I don't always hear immediately about new big contigs in the Saccharomyces or Caenorhabditis genomes. Keith Robison Harvard University Department of Cellular and Developmental Biology Department of Genetics / HHMI robison@mito.harvard.edu From owner-chromosomes@net.bio.net Sun Jan 15 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!daresbury!sunsite.doc.ic.ac.uk!agate!library.ucla.edu!csulb.edu!csus.edu!netcom.com!mickey From: mickey@netcom.com (Mickey Smith) Subject: ??? Genetic Engineer Career ??? Message-ID: Sender: mickey@netcom.com (Mickey Smith) Organization: NETCOM On-line Communication Services (408 261-4700 guest) X-Newsreader: WinVN 0.92.5 Date: Sun, 15 Jan 1995 19:54:28 GMT Lines: 37 I'm posting these question for my 14 year old nephew. He is writing a school report on genetic engineering as a career (I think that's what he want to do when he grows up). Please post answers to me mickey@netcom.com 1. What education is necessary to become a genetic engineer? 2. What are the earnings? Beginning salary? Lifetime? 3. What are the working conditions? 4. What are the opportunies for advancement? 5. What are some of the disadvantages of this job? 6. What are some advantages of becoming a genetic engineer? 7. What are some hazards of altering the genes of a living thing? 8. What living thing is the easiest genes to alter? 9. What is the process? 10. In your opinion, how far away are we from altering the genetic codes of a fetus? 11. In your opinion, do you feel that we have the right to tamper with the genetic codes of human beings? Please include name and title with your response, he will need this information for his report. Thanks in Advance Uncle Mickey From owner-chromosomes@net.bio.net Sun Jan 15 22:00:00 1995 Path: biosci!rolix.com!nathan From: nathan@rolix.com Newsgroups: bionet.genome.chromosomes Subject: DISSCUSSION GROUP Date: 15 Jan 1995 23:07:10 -0800 Organization: New Visions Inc. Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <9501160254.D1264PL@rolix.com> NNTP-Posting-Host: net.bio.net Hello..... i am a student in highschool. I would very much appriciate it if you would send me some information about the human genome project by 1/16/95.THANK U!!! Nate Maynard nathan@rolix.com nathan@nvi.nvi.net Again.... a response by 1/16/95 would be appriciated! From owner-chromosomes@net.bio.net Wed Jan 18 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!pipex!uunet!newsflash.concordia.ca!canopus.cc.umanitoba.ca!tribune.usask.ca!quartz.ucs.ualberta.ca!oldnews.ucalgary.ca!news.ucalgary.ca!honte.uleth.ca!botany.biology.uleth.ca!bain From: John Bain Subject: Geneticist Position - U. of Lethbridge Message-ID: <1995Jan19.020159.1144@honte.uleth.ca> X-Xxmessage-Id: X-Xxdate: Wed, 18 Jan 95 19:04:03 GMT Sender: news@honte.uleth.ca (News System) Organization: University of Lethbridge X-Useragent: Nuntius v1.1.1d12 Date: Thu, 19 Jan 1995 02:01:59 GMT Lines: 43 THE UNIVERSITY OF LETHBRIDGE Faculty of Arts and Science Department of Biological Sciences 1. TITLE: Applications are invited for a tenure-track appointment in Genetics at the level of Assistant Professor. The position will commence 1 July 1995 subject to budgetary approval. 2. QUALIFICATIONS: Ph.D. required by the appointment date. Post-doctoral experience, teaching experience, and evidence of ability to develop an externally funded research program will be assets. Researchers in plant population genetics and plant or microbial molecular genetics are particularly encouraged to apply. In accordance with Canadian Immigration Regulations, this advertisement is directed to Canadian citizens and permanent residents of Canada. The University aspires to hire individuals who have demonstrated potential for excellence in teaching, research, and scholarship. Women and men are encouraged to apply for these positions. 3. RESPONSIBILITIES: The appointee will be expected to participate in the undergraduate program, including teaching courses in general and molecular genetics, and to develop a strong research program. Opportunities for collaborative research with Agriculture Canada and for graduate student supervision exist. 4. SALARY (1994-95): Assistant Professor $37,350 minimum per annum. 5. APPLICATIONS: Applicants should submit a letter of application, including curriculum vitae, statement of research interests, statement of teaching philosophy, a maximum of three important and/or recent publications, and names of referees. Arrange for this material and three letters of recommendation to be sent to Dr. Gail R. Michener, Chairperson Department of Biological Sciences The University of Lethbridge 4401 University Drive Lethbridge, Alberta T1K 3M4 -E-mail: Michener@hg.uleth.ca 6. Effective Date: 1 July 1995 7. Closing Date: 28 February 1995 From owner-chromosomes@net.bio.net Wed Jan 18 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!daresbury!sunsite.doc.ic.ac.uk!uknet!bcc.ac.uk!mol9.ioo.bpmf.ac.uk!user From: t.keen@ucl.ac.uk (Jeffrey Keen) Subject: Pharmacia ALF problem Message-ID: Date: Thu, 19 Jan 1995 16:51:41 GMT Organization: University College London Lines: 16 Has anybody had the same problem as me with their Pharmacia ALF sequencer? What has developed is that I am getting a very high peak of fluorescense (way off scale) passing through the gel just before the primer peak. Pre-running the gel appears to allow the contamination to pass through the gel before loading, but still leaves a residual background of around 45%. The fluorescense isn't noisy so doesn't affect the sequence (as far as I can tell anyway). I've stopped using our own RO water and just use Merck Analar stuff without getting rid of the problem. Swapping detergents doesn't seem to help either. Any suggestions Jeffrey Keen t.keen@ucl.ac.uk From owner-chromosomes@net.bio.net Wed Jan 18 22:00:00 1995 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!qmw!IMMU-5.lhmc.ac.uk!SMIAH From: SMIAH@lhmc.ac.uk (LHMC Immunology Dept.) Newsgroups: bionet.genome.chromosomes Subject: garbage messages Date: Thu, 19 Jan 1995 18:00:02 UNDEFINED Organization: LHMC Lines: 7 Message-ID: NNTP-Posting-Host: immu-5.lhmc.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Sorry - my mailer was not working properly. Thanks From owner-chromosomes@net.bio.net Wed Jan 18 22:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!sol.ctr.columbia.edu!hamblin.math.byu.edu!news.Arizona.EDU!usenet From: gu@biosci.arizona.edu Newsgroups: bionet.genome.chromosomes Subject: human genome meeting Date: 19 Jan 1995 20:27:02 GMT Organization: university of arizona Lines: 1 Distribution: world Message-ID: <3fmhum$ijk@news.CCIT.Arizona.EDU> NNTP-Posting-Host: jimmy.biosci.arizona.edu X-Newsreader: From owner-chromosomes@net.bio.net Thu Jan 19 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!pipex!sunsite.doc.ic.ac.uk!qmw!IMMU-5.lhmc.ac.uk!S.Miah From: S.Miah@lhmc.ac.uk (LHMC Immunology Department, Walden Building.) Newsgroups: bionet.genome.chromosomes Subject: MHCDB Date: Fri, 20 Jan 1995 13:57:01 GMT Organization: London Hospital Medical College Lines: 8 Message-ID: NNTP-Posting-Host: immu-5.lhmc.ac.uk Keywords: MHCDB X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Hi, Is any one aware as to how to get access to MHCDB database. If so Please reply either here or mail me directly. Thanks! Shana From owner-chromosomes@net.bio.net Fri Jan 20 22:00:00 1995 Path: biosci!CMU.CHIANGMAI.AC.TH!mdbcs001 From: mdbcs001@CMU.CHIANGMAI.AC.TH (Wirote Tuntiwechapikul) Newsgroups: bionet.genome.chromosomes Subject: Email address of E.Baysal ? Date: 21 Jan 1995 00:43:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear netter, Does anyone there know email address of E.Baysal, Dept. of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia ? Thank you. Wirote Tuntiwechapikul. From owner-chromosomes@net.bio.net Fri Jan 20 22:00:00 1995 Path: biosci!BIOSCI.ARIZONA.EDU!GU From: GU@BIOSCI.ARIZONA.EDU Newsgroups: bionet.genome.chromosomes Subject: Human genome meeting Date: 20 Jan 1995 16:03:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <950120170756.20c1c2b8@biosci.arizona.edu> NNTP-Posting-Host: net.bio.net I will attend the 2nd annual human genome project commercial implications meeting, march 6-10, 1995. San francisco, CA. I want to reserve the Hotel Nikko. the meeting is hosted in there. The double room is $135. I am looking for a roommate. So we can reserve the room together. I am a male. If anyone interest, please drop a note at . Thank you. Kerong Gu From owner-chromosomes@net.bio.net Sun Jan 22 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!newsfeed.pitt.edu!dsinc!news.ee.vill.edu!shrsys.hslc.org!esmartin From: esmartin@shrsys.hslc.org Newsgroups: bionet.genome.chromosomes Subject: help!sequencing with ALF Date: 23 JAN 95 18:48:29 GMT Organization: MIT PLASMA FUSION CENTER Lines: 1 Message-ID: <23JAN95.18482907@shrsys.hslc.org> NNTP-Posting-Host: shrsys.hslc.org we have just purchased an ALF and would like some protocols and advice on getting us started on seq'ing 300-8000 bp products.E-mail us @esmartin@hslc.org or fax-302 733 3773. From owner-chromosomes@net.bio.net Mon Jan 23 22:00:00 1995 Path: biosci!GENE.BIOTECH.WISC.EDU!Tom_Keller From: Tom_Keller@GENE.BIOTECH.WISC.EDU ("Tom Keller") Newsgroups: bionet.genome.chromosomes Subject: OJ case Date: 24 Jan 1995 13:08:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199501242108.NAA11307@net.bio.net> NNTP-Posting-Host: net.bio.net OJ case Does anyone know what systems were used for the DNA evidence in the OJ case? Are the results public (or known outside)? From owner-chromosomes@net.bio.net Mon Jan 23 22:00:00 1995 Path: biosci!UCS.INDIANA.EDU!JCLAY From: JCLAY@UCS.INDIANA.EDU ("Jane Clay, Continuing Studies, 5-6329") Newsgroups: bionet.genome.chromosomes Subject: DNA COURSE OFFERINGS Date: 24 Jan 1995 13:32:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 221 Sender: daemon@net.bio.net Distribution: world Message-ID: <199501242132.NAA12689@net.bio.net> NNTP-Posting-Host: net.bio.net The following message contains information about two DNA course offerings at Indiana University, including a registration form that may be printed, completed, and returned. Recombinant DNA: Techniques and Application This summer, Indiana University will offer two laboratory-based short courses that provide the opportunity to learn and apply recombinant DNA procedures and the technologies of sequencing, PCR analysis, and RFLP analysis. Courses dealing with the technology of DNA analysis are in their thirteenth year at Indiana University, and they have received high praise for excellent instruction and immediate applicability. The application of this technology is relatively easy to understand and is comparatively economical in time, equipment, and materials. Participants in these courses will learn to perform these techniques and may arrange to work on a sample of genomic DNA from their own research organism. Participants are expected to include * college or high school educators who will learn to perform and teach these techniques. * industrial or other laboratory researchers considering the application of recombinant DNA technology. * others interested in the application of this technology in society. Special features of these courses * Participants will have the opportunity during the courses to isolate and work with genomic DNA from their own research organism. Call 812-855-6329 to make the necessary arrangements. * Participants will receive a registration discount when they enroll in both courses. In these courses, learn techniques and applications that will allow you to * advance your own research. * isolate and/or sequence DNA that you bring. * obtain valuable experience for teaching DNA technology in the classroom. * gain familiarity with the organization of the human genome. I. Recombinant DNA Technology June 4-9, 1995 Participants in this course will learn the following techniques: * DNA and cloning vector manipulation * PCR technology * Preparation of recombinant DNA * Transformation of bacterial cells * Selection and assay of cloned and amplified fragments of "foreign" DNA * Transfer of DNA for probing (Southern blot) * Preparation of nonradioactive DNA probes This course is designed for those with a basic understanding of the structure of DNA and with a minimal understanding of enzymes and biochemistry. Most of the procedures taught to biology graduate students in the recombinant DNA section of a graduate techniques course at Indiana University will be covered. II. Application of Recombinant DNA Technology: RFLP and Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing June 11-16, 1995 The following techniques will be emphasized: * DNA sequencing * RAPD analysis of genomic DNA * Fingerprinting and RFLP analysis of genomic DNA * Electroporation of bacterial cells * Chemiluminescent detection of nucleic acids * Application of computers to DNA sequencing data analysis * Preparation of random fragment sequencing libraries and double stranded DNA for sequencing * Use of BioNeb cell and biopolymer disruption systems This course is designed for those with a basic understanding of the structure of DNA and with a minimal understanding of enzymes and biochemistry. Previous experience with PCR analysis, RFLP analysis, and DNA sequencing is not a prerequisite, nor is completion of the Recombinant DNA Technology course. Faculty The instructor for these courses will be Dr. Stefan J. Surzycki, associate professor of biology at Indiana University. Dr. Surzycki teaches laboratory classes employing the techniques introduced in these courses. His research focuses on development of large-scale DNA sequencing techniques, computer analysis, and genomic screening. Dr. Surzycki is also co-inventor of the BioNeb cell disruption system, a device that gently breaks cells and randomly shears DNA into uniform fragments of chosen size. The BioNeb was named one of the 100 most technologically significant new products of 1993. Dr. Surzycki will be assisted in each course by Judith Surzycki, a teaching associate in biology with extensive experience teaching the techniques of these courses, and by an advanced doctoral student in biology. Schedule Each short course will open with a Sunday evening reception. In addition to the daytime laboratory schedule, lectures and continued lab work are planned for three evenings. Location Bloomington, home of Indiana University, is a town of 60,000 located 50 miles southwest of Indianapolis. Bloomington offers the hospitality of a small town with the entertainment, shops, and restaurants of a city. Indiana University's wooded campus is surrounded by hills and lakes that provide many recreational opportunities. Housing Hotel accommodations and campus dormitory housing are available for each program, and room rates are moderate. Detailed information about travel routes to Bloomington, accommodations, meals, and parking will be sent to participants as soon as registrations are received. Tuition The fee for each course is US$925. Participants in both courses pay a total discounted fee of US$1,500. The fee includes all instruction, laboratory supplies, use of equipment, and an extensive lab manual in each class. An opening reception and coffee breaks are also included. Housing, parking, and meals are not included. Registration Registration deadline is May 19, 1995, for both courses. Enrollment is limited. Continuing Education Units Four continuing education units (CEUs) will be earned for completion of each program. CEUs are nationally recognized units earned through participation in certified noncredit continuing education programs. Registration Form (to be printed, filled out, and returned) (deadline May 19, 1995) Please check appropriate box(es). / / I. Recombinant DNA Technology (June 4-9) / / II. Application of Recombinant DNA Technology: RFLP and Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing (June 11-16) Name _____________________________________ Position ___________________________________ Organization or School __________________________________ Address ___________________________________ City ______________________________________ State ____________ Zip _____________________ Work Phone ________________________________ Home Phone _______________________________ Fee for one program: US$925 Discounted fee for both programs: US$1,500 Amount enclosed: US$________________ Make check or money order payable to Indiana University. All or part of the cost of this program may be tax deductible. For further information, contact your local IRS office or your tax consultant. / / VISA / / MasterCard / / Discover Credit Card Number ___________________________ Expiration Date ______________________________ Name on Card (Please print) __________________________________________ Signature of Card Holder __________________________________________ Mail your registration and check (made payable to Indiana University) to: Division of Continuing Studies, Owen Hall 204, Indiana University, Bloomington, IN 47405 or fax your registration (with completed credit card information) to Jane Clay at 812-855-8997 For additional information contact: Jane Clay, Division of Continuing Studies Phone: 812-855-6329 Fax: 812-855-8997 Internet: JClay@Indiana.EDU or Dr. Stefan Surzycki, Department of Biology Phone: 812-855-4880 From owner-chromosomes@net.bio.net Tue Jan 24 22:00:00 1995 Path: biosci!news.Stanford.EDU!agate!howland.reston.ans.net!news.sprintlink.net!pipex!sunsite.doc.ic.ac.uk!dundee.ac.uk!bioch8.medschool.dundee.ac.uk!bshek From: bshek@ninewells.dundee.ac.uk (Benedicte Shek) Newsgroups: bionet.genome.chromosomes Subject: rtpcr Date: Wed, 25 Jan 1995 13:36:12 GMT Organization: University of Dundee Lines: 4 Message-ID: NNTP-Posting-Host: bioch8.medschool.dundee.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hi, has anyone out there ever tried doing RTPCR using pfu polymerase instead of Taq polymerase and if so, does it work? I would very much appreciate any advice/help. Thank you. B.S. From owner-chromosomes@net.bio.net Wed Jan 25 22:00:00 1995 Path: biosci!DXI.NIH.GOV!goldman%bchem.dnet From: goldman%bchem.dnet@DXI.NIH.GOV Newsgroups: bionet.genome.chromosomes Subject: pcr taq pfu Date: 26 Jan 1995 13:57:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9501262156.AA24623@dxi.nih.gov> NNTP-Posting-Host: net.bio.net Have tried pfu in straight pcr. it is a very unreliable and fussy enzyme, but has the advantage of NO 3' transferase activity, and 3'-5' nuclease activity for error correction. If you spike Taq with 1/100 pfu you keep the high 'processiveness' of Taq and gain the editing function of pfu. ASHG From owner-chromosomes@net.bio.net Wed Jan 25 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!usenet.ucs.indiana.edu!raven.bio.indiana.edu!user From: lwashing@sunflower.bio.indiana.edu (Lawrence Washington) Newsgroups: bionet.genome.chromosomes Subject: Re: Pharmacia ALF problem Date: 26 Jan 1995 19:49:08 GMT Organization: Indiana University Lines: 26 Message-ID: References: NNTP-Posting-Host: raven.bio.indiana.edu In article , t.keen@ucl.ac.uk (Jeffrey Keen) wrote: > Has anybody had the same problem as me with their Pharmacia ALF sequencer? > > What has developed is that I am getting a very high peak of fluorescense > (way off scale) passing through the gel just before the primer peak. > Pre-running the gel appears to allow the contamination to pass through the > gel before loading, but still leaves a residual background of around 45%. > The fluorescense isn't noisy so doesn't affect the sequence (as far as I > can tell anyway). > I've stopped using our own RO water and just use Merck Analar stuff > without getting rid of the problem. Swapping detergents doesn't seem to > help either. > > Any suggestions Sorry, I have no suggestions. But I am curious about something. How did Pharmacia tech support respond when you asked them about this? I am just wondering how they are to deal with in matters of trouble shooting. -- Lawrence Washington Indiana University Institute for Molecular and Cellular Biology From owner-chromosomes@net.bio.net Thu Jan 26 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!nott!nrcnet0.nrc.ca!ratilal From: "Christoph W. Sensen" Newsgroups: bionet.genome.chromosomes Subject: Re: Pharmacia ALF problem Date: 27 Jan 1995 14:58:07 GMT Organization: National Research Council, Canada Lines: 38 Message-ID: <3gb1lv$pc0@nrcnet0.nrc.ca> References: NNTP-Posting-Host: sensen.imb.nrc.ca lwashing@sunflower.bio.indiana.edu (Lawrence Washington) wrote: > > In article , t.keen@ucl.ac.uk > (Jeffrey Keen) wrote: > > > Has anybody had the same problem as me with their Pharmacia ALF sequencer? > > > > What has developed is that I am getting a very high peak of fluorescense > > (way off scale) passing through the gel just before the primer peak. > > Pre-running the gel appears to allow the contamination to pass through the > > gel before loading, but still leaves a residual background of around 45%. > > The fluorescense isn't noisy so doesn't affect the sequence (as far as I > > can tell anyway). > > I've stopped using our own RO water and just use Merck Analar stuff > > without getting rid of the problem. Swapping detergents doesn't seem to > > help either. > > > > Any suggestions May be that I have a suggestion for you: You are obviously introducing fluorescein into your sequencing system somewhere. If it is not in the chemicalss, then check out the following potential sources: - permanet pens (especially red ones) used to write things on the glass plates prior to gel-loading - Mr. Clean and his relatives used to wash the glass plates (the greenish colour is often fluorescein) - Do you use absolute Ethanol only or is there a contamination in your Enthanol sources somewhere (check on UV plate) these are the three most obvious sources of contaminations. If you do not find the flourecein there, then check everything else that you use on the UV plate and see wahat is lightening up.... Christoph Sensen From owner-chromosomes@net.bio.net Fri Jan 27 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!nntp.crl.com!crl3.crl.com!not-for-mail From: dspodick@crl.com (David Spodick) Newsgroups: bionet.genome.chromosomes Subject: Re: Pharmacia ALF problem Date: 27 Jan 1995 20:03:18 -0800 Organization: CRL Dialup Internet Access (415) 705-6060 [Login: guest] Lines: 35 Message-ID: <3gcfm6$n4p@crl3.crl.com> References: NNTP-Posting-Host: crl3.crl.com X-Newsreader: TIN [version 1.2 PL2] Jeffrey Keen (t.keen@ucl.ac.uk) wrote: : Has anybody had the same problem as me with their Pharmacia ALF sequencer? : What has developed is that I am getting a very high peak of fluorescense : (way off scale) passing through the gel just before the primer peak. : Pre-running the gel appears to allow the contamination to pass through the : gel before loading, but still leaves a residual background of around 45%. : The fluorescense isn't noisy so doesn't affect the sequence (as far as I : can tell anyway). : I've stopped using our own RO water and just use Merck Analar stuff : without getting rid of the problem. Swapping detergents doesn't seem to : help either. : Any suggestions : Jeffrey Keen : t.keen@ucl.ac.uk Jeffrey, I am currently at a meeting of ALF specialists for North America. We haved discussed your problem and would like to follow up with a few questions. How many times has this happened? Does it matter what primer you use? What kind of gel are you running (acrylamide or LongRanger)? Have you changed any gel components lately? It is possible that certain batches of APS or Urea may have some of the contaminants that you describe. You can respond to me personally or through this newsgroup. David Spodick, ALF Specialist Pharmacia Biotech dspodick@crl2.crl.com From owner-chromosomes@net.bio.net Fri Jan 27 22:00:00 1995 Path: biosci!GENOME.GENETICS.UGA.EDU!arnold From: arnold@GENOME.GENETICS.UGA.EDU Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 27 Jan 1995 10:31:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 165 Sender: daemon@net.bio.net Distribution: world Message-ID: <0098B172.BF028A27.78@genome.genetics.uga.edu> Reply-To: arnold@bscr.uga.edu Measuring physical map quality using bootstrap resampling PROGRAM DESCRIPTION: This program is to assist in measuring the reconstructed physical map quality using bootstrap resampling. The theory for reconstruction of chromosomes or chromosome fragments ("contig mapping") from a clonal library can be found in Cuticchia, A.J., Arnold, J., and W.E. Timberlake. (1992a). The use of simulated annealing in chromosome reconstruction experiments based on binary scoring. Genetics 132: 591-601 The program that orders DNA sequences is based on similarity of their binary profiles assigned to clones in a library by one of several experimental approaches. The algorithm has been used to map the Schizosaccharomyces pombe genome, the Aspergillus nidulans genome, and a region of Human chromosome IX. DNA fragments with a high degree of overlap are expected to show a high degree of similarity in their profiles. The ordering process is based on minimizing the sum of the linking distances between clones as a function of their ordering along the chromosome. This minimization algorithm used here is a new one called random cost. It is detailed in Wang, Y., Prade, R. A., Griffith, J., Timberlake, W.E., and Arnold, J. (1994) A fast random cost algorithm for physical mapping. PNAS, 91, 11094-11098 In bootstrap resampling, probes are randomly resampled with replacement for many times (1000 default). This program calculates how often the links in the original reconstructed map reappear in maps under bootstrap resampling. Three such frequencies are calculated; they are: (i) how often two clones appear together- C1; (ii) how often a clone or one that is equivalent in hybridization profile appear next to each other - C2; (iii) how often two clones are within the same island - C3. A description of the bootstrap resampling procedure for assessing the reliability of a physical map is described in: Wang, Y., Prade, R.A., Griffith, J., Timberlake, W.E., and Arnold, J. (1994) ODS_BOOTSTRAP: assessing the statistical reliability of a physical map by bootstrap resampling. CABIOS 10: 625-634 PROGRAM INPUT A typical batch file is given as follows: $set def [wang.cm.bootstrap] $run boot boot.dat probe.dat boot.out boot.rec 0 The first line is to set the default directory. The second line is to run this program The third line is the input file name. In the input hybridization file, the first line should be the number of probes, clones and bootstrap run numbers. For all other lines, the first ten columns are reserved for the clone, name and the hybridization data should start at any column after 10th column. Total length of each line is defined by MAX_BUFFER in the program. The example is the clone/probe hybridization matrix for Chromosome IV of A. nidulans. The fourth line is the probe names file name. It contains the probe names in the same order across the columns as in the input file. The fifth line is the output file name. The finally reconstructed physical map, the statistical confidence statistics from the bootstrap run and other statistics are written to this file. The sixth line is the name of the file that stores the total linking distance for each bootstrap run. This is for tracing how the program is running. The seventh line is the seed for random number generator. PROGRAM INPUT LIMITATIONS: The number of clones must be between 1 and 600 for a DEC VAX station 4000. Filenames (with directory path, if specified) must be no longer than 40 characters. If the bootstrap run number is not given in the input binary hybridization data file, it will be set to the default number of 1000. PROGRAM SPEED: The program assembled a physical map of 593 clones probed with 115 probes within less than 2 mins on a DEC VAX station 4000. Total time for 1000 bootstraps run will take 30 CPU hours on this workstation. OBTAINING THE SOFTWARE: The software is only distributed via Internet using EMAIL. Please send an EMAIL request to: ARNOLD@BSCR.UGA.EDU if you wish copies of the program. I will EMAIL you: 1) a C program boot.c; 2) this documentation file, boot.DOC; 3) a test input file, boot.dat; 4) a test probe name file, probe.dat 4) an example output file, boot.dat; and 5) a command file, boot.COM. This last file is what you would use to submit a batch job in the VAX/VMS operating system to generate the file boot.out. SOFTWARE SUPPORT IN THE USE OF THE PROGRAMS: If you have questions about the programs, please contact Yuhong Wang currently located at University of Georgia: wang@bscr.uga.edu or myself at arnold@bscr.uga.edu HARDWARE LIMITATIONS: The programs have been run without modification on VAXstations, a DECstation 3100, a Silicon Graphics IRIS 4D70/GT workstation and IBM Risc 6000 workstation. . - - - - - - - - - - - Jonathan Arnold - - - - - - - - - - - - - - - . | Dept. of Genetics, | | University of Georgia | | Athens, Georgia 30602 | | Phone: (706) 542-1449 | | messages: (706) 542-8000 | | FAX: (706) 542-3910 | | Internet: ARNOLD@BSCR.UGA.EDU | . - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - . From owner-chromosomes@net.bio.net Fri Jan 27 22:00:00 1995 Path: biosci!JEEVES.UCSD.EDU!hoschek From: hoschek@JEEVES.UCSD.EDU (gisela hoschek) Newsgroups: bionet.genome.chromosomes Subject: proof-reading English papers Date: 27 Jan 1995 12:35:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <9501272013.AF19192@jeeves.UCSD.EDU> Is English your second language? Editors and reviewers let me know that there is a need for help to authors of research papers to be submitted for publication in English or for grant applications to English speaking sources. I would like to help (translating and/or proof-reading) where my expertise qualifies me: molecular biology, biochemistry, molecular genetics. I am not looking for employment, but would like to work with individual authors. Since I recently retired from the lab bench for health reasons, I want to stay connected and active, and put to use the skills and knowledge I acquired over the last 20 years. I have worked mainly in the field of plant molecular genetics (UCLA and UCSD), with excursions into entomology (bacculoviruses) and cancer research (in tissue culture). I speak German fluently, and French enough to understand. For more INFO please contact me: Gisela Hoschek 1124 Nardo Road Encinitas, CA 92024 USA e-mail: hoschek@jeeves.ucsd.edu tel.: (619) 944-4233 From owner-chromosomes@net.bio.net Sun Jan 29 22:00:00 1995 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!dundee.ac.uk!bioch8.medschool.dundee.ac.uk!bshek From: bshek@ninewells.dundee.ac.uk (Benedicte Shek) Newsgroups: bionet.genome.chromosomes Subject: RTPCR Date: Mon, 30 Jan 1995 11:05:47 GMT Organization: University of Dundee Lines: 4 Message-ID: NNTP-Posting-Host: bioch8.medschool.dundee.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Has anyone out there ever used pfu polymerase instead of Taq polymerase in RTPCR? If so does it work and if not then why not?! Cheers B.S. From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!ix.netcom.com!netnews From: mmyers@ix.netcom.com (molly myers) Newsgroups: bionet.genome.chromosomes Subject: Re: CSH Genome Sequencing Course Date: 31 Jan 1995 15:01:01 GMT Organization: Netcom Lines: 289 Distribution: world Message-ID: <3gljbd$dot@ixnews3.ix.netcom.com> References: <9501132201.AA01898@watson.wustl.edu> NNTP-Posting-Host: ix-dc2-20.ix.netcom.com In <9501132201.AA01898@watson.wustl.edu> rwilson@WATSON.WUSTL.EDU (Rick Wilson) writes: > >Cold Spring Harbor Laboratory > >ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 > >Correspondence should be addressed to: >""""""""""""""""""""""""""""""""""""""""""""" >Cold Spring Harbor Laboratory >The Meetings Office >1 Bungtown Road, P.O. Box 100 >Cold Spring Harbor >New York 11724-2213 > >Phone: (meetings) 516 367 8346 > (courses) 516 367 8345 >Fax: 516 367 8845 >E-mail: meetings@cshl.org >World wide web site at http://www.cshl.org/ >""""""""""""""""""""""""""""""""""""""""""""" >CONTENTS > >1) Introduction >2) Full description of course (dates, instructors, description, application deadline) >3) Short list of meetings and courses >4) Information for course applicants >5) 1995 course price >6) Sponsors of the 1994 Courses Program >7) Getting to Cold Spring Harbor Laboratory >8) General information about Cold Spring Harbor Laboratory > >Application forms and further information can be obtained from the above address. > >1) Introduction > >Cold Spring Harbor Laboratory is a non-profit independent research and educational institution chartered by the University of the State of New York. The Laboratory employs 550 people, including a research staff of almost 200 Ph.D. scientists and 50 graduate students. Cold Spring Harbor Laboratory's primary goal is to carry out first-rate scientific research. However, the Laboratory pursues other goals which we believe are vital to the well-being of science and scientists: scientific communication; training scientists; public education; and publishing. The Laboratory is located thirty-five miles east of New York City on the scenic north shore of Long Island. > >Each year, over 5,000 scientists worldwide attend one or several of the more than 15 scientific meetings held at Cold Spring Harbor Laboratory in the award- winning Grace Auditorium. The flagship of this program is the annual Symposium on Quantitative Biology, one of the most prestigious meetings in molecular biology. Professional meetings are an integral part of a scientist's job; they are a primary means by which scientists stay current on the latest results and techniques in their field. > >Complementing our meetings program is our annual program of 25 high-level courses. Directed primarily at post-doctoral level scientists, these intensive courses allow practicing researchers to immerse themselves in new techniques and ideas that they can apply immediately to their own research. Many of these courses are laboratory courses held in our Beckman Neuroscience Center, which was completed in 1991. For more than 50 years, courses at Cold Spring Harbor have trained the rising stars in emerging disciplines and shaped the direction of research, both at Cold Spring Harbor and elsewhere. > >2) Full Description of Course > >Application Deadline January 31, 1995 > >ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 > >Ellson Y. Chen, Perkin Elmer Corporation >Richard Gibbs, Baylor College of Medicine >W. Richard McCombie, Cold Spring Harbor Laboratory >Richard K. Wilson, Washington University > >Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequencers to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution. Students will work in groups to sequence a large region of DNA and through this process be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA! > sequencing. > >3) Short list of 1995 meetings and courses > >1995 Meetings (in chronological order) > >Molecular and Behavioral Biology of Aplysia and Related Molluscs >The Cytoskeleton & Cell Function >Tyrosine Phosphorylation & Cell Signalling >Genome Mapping & Sequencing >RNA Processing >Retroviruses >Symposium: Protein Kinesis - The Dynamics of Protein Trafficking & Stability >Yeast Cell Biology >Molecular Genetics of Bacteria & Phages >Cancer Cells: Mechanisms of >Eukaryotic Transcription >Eukaryotic DNA Replication >Molecular Approaches to the Control of Infectious Diseases >Programmed Cell Death >Plant Signalling in Development >Neurobiology of Drosophila > >1995 Courses (in alphabetical order) > >Advanced Bacterial Genetics >Advanced Genome Sequence Analysis >Advanced In Situ Hybridization & Immunocytochemistry >Advanced Molecular Cloning & Expression of Eukaryotic Genes >Arabidopsis Molecular Genetics >Cloning and Analysis of Large DNA Molecules >Computational Genomics >Early Development of Xenopus Laevis >Macromolecular Crystallography >Molecular Embryology of the Mouse >Molecular Markers for Plant Breeding & Plant Genetics >Phage Display of Combinatorial Antibodies >Protein Purification & Characterization >YACs in Structural and Biological Genome Analysis >Yeast Genetics > >Courses in Neurobiology > >Brain Mapping >Developmental Neurobiology >Imaging Structure and Function in the Nervous System >Molecular Approaches to Ion Channel and Function >Molecular Cloning of Neural Genes >Neurobiology of Drosophila >Neurobiology of Human Neurological Disease: Mechanisms of Human Neurodegeneration >Structure, Function & Development of the Visual System >The Biology of Memory: From Molecules to Behavior > >4) Information for Course Applicants > >Registration: >Mail the application form plus 3 additional copies of application and accompanying material to the Course Registrar on or before the course deadline. If applying for more than one course, a separate application form plus 3 additional copies must be submitted for each course. Please select one course per session. > >Selection Process: >Each year, the instructors are confronted with the difficult task of selecting the students for our courses from a large number of well-qualified applicants. To facilitate the application process, students are asked to provide information documenting their request for a place in the course. First and foremost, applicants should give their reasons for wanting to take the course. As part of this, you should briefly outline your career. It is highly recommended that you supplement your application with one or more letters of recommendation. Applicants should remember that selection is based upon the degree to which they would benefit from this training opportunity; this in turn will be judged from the information given by the applicants. > >Applicants will be notified by mail whether or not they have been accepted to attend. The decisions often take several weeks after the application deadline to be finalized and announced. > >Stipends: > >Scholarship support is available to all qualified applicants based on need. Any candidate requesting financial assistance MUST request it when submitting the application. Attach a separate letter stating the amount requested and give a full justification for the required support. The amount awarded will be based upon the availability of funding. > >Housing: >All students must live in the Laboratory dormitories and take meals in the dining room. Most of the rooms are multiple occupancy and/or shared bath. Please be sure to indicate gender on the application form. Due to space limitations, families cannot be housed on grounds; there are no exceptions to this policy! > >Course Application Checklist: >Mail original plus 3 copies of the following: >** Original application for each course applied for. >** Letter and/or curriculum vitae outlining your career including reasons why taking the course would benefit you >** Letter(s) of recommendation >**(Stipend request) > >5) Prices for 1995 Courses: >Length of Course Cost * > >6 Days $1,155.00 >1 Week $1,215.00 >10 Days $1,460.00 >2 Weeks $1,720.00 <= COST OF THIS COURSE >3 Weeks $2,180.00 > >* Cost includes tuition, food and housing > >6) Sponsors of the 1994 Courses Program (not specifically for this course) > >The Howard Hughes Medical Institute ** The Esther A. & Joseph Klingenstein Fund, Inc. ** United States Department of Agriculture ** United States Department of Energy ** National Cancer Institute ** National Center of Human Genome Research ** National Institute of General Medical Sciences ** National Institute of Mental Health ** National Institutes of Health ** National Science Foundation > >7) Getting to Cold Spring Harbor Laboratory > >** New York Airports: J.F. Kennedy, La Guardia and L.I. MacArthur Airports are most convenient to the Laboratory (allow 1 1/2 - 2 hours travel time each way). Cold Spring Harbor Laboratory does not coordinate travel arrangements from local airports. >** Limousines: Prices for JFK and LaGuardia only! >Syosset Limousine - 516-364-9681, reservation required. Private Car (1 - 9 people). Cost = $65.00 for 1 person, $33.00 pp. for 2, $25.00 pp. for 3 or more. >Classic Limousine - 516-567-5100 (or 1-800-666-4949 outside New York). Shared Ride Service (possible stops at other terminals/destinations). Use courtesy phone at airport. No reservation required. Cost = $33.00 per person. >Other transportation options are available at the airports. Do not use taxicabs for transportation from the airport to CSHL. >** Railroad: Long Island Railroad is located in Penn Station. Take the PT JEFFERSON BRANCH train to SYOSSET. Note: Possible transfer at Jamaica Station. Taxis usually meet arriving trains at Syosset station ticket office. Syosset Taxi 921-2141; $6.50 for 1 person, $4.00 each additional person. >** Automobile: From the Long Island Expressway (Rte 495), >Exit 41 North (Route 106-107). On 106-107, bear right at fork (Route 106 - Oyster Bay). Travel 3.5 miles on Rte 106, make a right turn (East) onto Route 25A (town of East Norwich). Travel 4 miles. The lab entrance is on the left. (A gold Victorian house marks the entrance). >** New York - Connecticut Ferry Services: >Port Jefferson - Bridgeport (516-473-0286) >Orient Point - New London (516-323-2743) > >8) General Information about services at Cold Spring Harbor Laboratory > >** Registration: Please register at Grace Auditorium upon your arrival at the laboratory. Meetings begin at 7:30 in the evening. Dinner is served from 5:30 to 7:30 prior to meetings. Please call ahead if you plan to arrive at the lab after midnight. >** Parking: All vehicles MUST receive a Temporary Parking Permit from The Meetings Office. Any vehicle on grounds without such a permit will be subject to booting or towing. >** Phones / Faxes: Participants can receive faxes at the Meetings Office (516) 367-8845. Payphones and house phones are located throughout the campus. >Bookstore: The Bookstore is located in the lower level of Grace Auditorium. Store hours are posted. >** Computer Facilities: Lower level of Grace Auditorium, equipped with PC, Mac & laser printer. To access your e-mail, you must know the name of your home server. > >""""""""""""""""""""""""""""""""""""""""""""" >Correspondence should be addressed to: > >Cold Spring Harbor Laboratory >The Meetings Office >1 Bungtown Road, P.O. Box 100 >Cold Spring Harbor >New York 11724-2213 > >Phone: (meetings) 516 367 8346 > (courses) 516 367 8345 >Fax: 516 367 8845 >E-mail: meetings@cshl.org >World wide web site at http://www.cshl.org/ >""""""""""""""""""""""""""""""""""""""""""""" > I am looking for current research being done on breast cancer under human genome project. please reply to mmyers@ix.netcom.com From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!Germany.EU.net!EU.net!Austria.EU.net!newsfeed.ACO.net!fuw.edu.pl!news.nask.org.pl!usenet From: andy@psd.ibb.waw.pl (Andrzej Migdalski) Newsgroups: bionet.genome.chromosomes Subject: Re: Pharmacia ALF problem Date: 31 Jan 1995 12:42:11 GMT Organization: Institute of Biochemistry and Biophysics PAS Lines: 50 Distribution: world Message-ID: <3glb73$dfm@bilbo.nask.org.pl> References: Reply-To: andy@psd.ibb.waw.pl NNTP-Posting-Host: psd.ibb.waw.pl In article , lwashing@sunflower.bio.indiana.edu (Lawrence Washington) writes: >In article , t.keen@ucl.ac.uk >(Jeffrey Keen) wrote: > >> Has anybody had the same problem as me with their Pharmacia ALF sequencer? >> >> What has developed is that I am getting a very high peak of fluorescense >> (way off scale) passing through the gel just before the primer peak. >> Pre-running the gel appears to allow the contamination to pass through the >> gel before loading, but still leaves a residual background of around 45%. >> The fluorescense isn't noisy so doesn't affect the sequence (as far as I >> can tell anyway). >> I've stopped using our own RO water and just use Merck Analar stuff >> without getting rid of the problem. Swapping detergents doesn't seem to >> help either. >> >> Any suggestions We had identical problem. I don't know the reason, it dissappeared after we had washed the notched plates with 5% KOH in methanol but it could be just coincidence (background never reappeared even after stadard plate washing). My friend believes its the problem of ethanol impurities and careful washing with water after the ethanol will help. >Sorry, I have no suggestions. But I am curious about something. How did >Pharmacia tech support respond when you asked them about this? I am just >wondering how they are to deal with in matters of trouble shooting. Oh yes, Pharmacia support! Our local Pharmacia support could solve only hardware problems, they knew nothing about molecular biology or sequencing at least. They even did not know how to make acrylamide gels (for several years we were the only ALF users in Poland so they had to learn with us). ------------ GCT TAA ATG ATT GGT GAT GCT TTG TCT AAG ATT ------------- Andrzej Migdalski Tel (48-2) 658 12 24 DNA Sequencing Lab. Fax (48) 39 12 16 23 Institute of Biochemistry & Biophysics Email: andy@psd.ibb.waw.pl Polish Academy of Sciences andy@slc.ibb.waw.pl Pawinskiego 5a, 02-106 Warsaw, Poland andy@ibbrain.ibb.waw.pl ------------------------------------------------------------------------ From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!pipex!warwick!bham!med252.bham.ac.uk!user From: a.buchan@bham.ac.uk (a.buchan) Newsgroups: bionet.genome.chromosomes Subject: 3 alpha hydroxysteroid dehydrogenase Followup-To: bionet.genome.chromosomes Date: 31 Jan 1995 12:04:49 GMT Organization: university of birmingham Lines: 3 Distribution: world Message-ID: NNTP-Posting-Host: med252.bham.ac.uk Does anyone know the chromosome location of the enzyme 3 alpha hydroxysteroid dehydrogenase? A.Buchan From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!ix.netcom.com!netnews From: mmyers@ix.netcom.com (molly myers) Newsgroups: bionet.genome.chromosomes Subject: Re: CSH Genome Sequencing Course Date: 31 Jan 1995 15:01:01 GMT Organization: Netcom Lines: 289 Distribution: world Message-ID: <3gljbd$dot@ixnews3.ix.netcom.com> References: <9501132201.AA01898@watson.wustl.edu> NNTP-Posting-Host: ix-dc2-20.ix.netcom.com In <9501132201.AA01898@watson.wustl.edu> rwilson@WATSON.WUSTL.EDU (Rick Wilson) writes: > >Cold Spring Harbor Laboratory > >ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 > >Correspondence should be addressed to: >""""""""""""""""""""""""""""""""""""""""""""" >Cold Spring Harbor Laboratory >The Meetings Office >1 Bungtown Road, P.O. Box 100 >Cold Spring Harbor >New York 11724-2213 > >Phone: (meetings) 516 367 8346 > (courses) 516 367 8345 >Fax: 516 367 8845 >E-mail: meetings@cshl.org >World wide web site at http://www.cshl.org/ >""""""""""""""""""""""""""""""""""""""""""""" >CONTENTS > >1) Introduction >2) Full description of course (dates, instructors, description, application deadline) >3) Short list of meetings and courses >4) Information for course applicants >5) 1995 course price >6) Sponsors of the 1994 Courses Program >7) Getting to Cold Spring Harbor Laboratory >8) General information about Cold Spring Harbor Laboratory > >Application forms and further information can be obtained from the above address. > >1) Introduction > >Cold Spring Harbor Laboratory is a non-profit independent research and educational institution chartered by the University of the State of New York. The Laboratory employs 550 people, including a research staff of almost 200 Ph.D. scientists and 50 graduate students. Cold Spring Harbor Laboratory's primary goal is to carry out first-rate scientific research. However, the Laboratory pursues other goals which we believe are vital to the well-being of science and scientists: scientific communication; training scientists; public education; and publishing. The Laboratory is located thirty-five miles east of New York City on the scenic north shore of Long Island. > >Each year, over 5,000 scientists worldwide attend one or several of the more than 15 scientific meetings held at Cold Spring Harbor Laboratory in the award- winning Grace Auditorium. The flagship of this program is the annual Symposium on Quantitative Biology, one of the most prestigious meetings in molecular biology. Professional meetings are an integral part of a scientist's job; they are a primary means by which scientists stay current on the latest results and techniques in their field. > >Complementing our meetings program is our annual program of 25 high-level courses. Directed primarily at post-doctoral level scientists, these intensive courses allow practicing researchers to immerse themselves in new techniques and ideas that they can apply immediately to their own research. Many of these courses are laboratory courses held in our Beckman Neuroscience Center, which was completed in 1991. For more than 50 years, courses at Cold Spring Harbor have trained the rising stars in emerging disciplines and shaped the direction of research, both at Cold Spring Harbor and elsewhere. > >2) Full Description of Course > >Application Deadline January 31, 1995 > >ADVANCED GENOME SEQUENCE ANALYSIS March 14 - 27, 1995 > >Ellson Y. Chen, Perkin Elmer Corporation >Richard Gibbs, Baylor College of Medicine >W. Richard McCombie, Cold Spring Harbor Laboratory >Richard K. Wilson, Washington University > >Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequencers to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution. Students will work in groups to sequence a large region of DNA and through this process be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA! > sequencing. > >3) Short list of 1995 meetings and courses > >1995 Meetings (in chronological order) > >Molecular and Behavioral Biology of Aplysia and Related Molluscs >The Cytoskeleton & Cell Function >Tyrosine Phosphorylation & Cell Signalling >Genome Mapping & Sequencing >RNA Processing >Retroviruses >Symposium: Protein Kinesis - The Dynamics of Protein Trafficking & Stability >Yeast Cell Biology >Molecular Genetics of Bacteria & Phages >Cancer Cells: Mechanisms of >Eukaryotic Transcription >Eukaryotic DNA Replication >Molecular Approaches to the Control of Infectious Diseases >Programmed Cell Death >Plant Signalling in Development >Neurobiology of Drosophila > >1995 Courses (in alphabetical order) > >Advanced Bacterial Genetics >Advanced Genome Sequence Analysis >Advanced In Situ Hybridization & Immunocytochemistry >Advanced Molecular Cloning & Expression of Eukaryotic Genes >Arabidopsis Molecular Genetics >Cloning and Analysis of Large DNA Molecules >Computational Genomics >Early Development of Xenopus Laevis >Macromolecular Crystallography >Molecular Embryology of the Mouse >Molecular Markers for Plant Breeding & Plant Genetics >Phage Display of Combinatorial Antibodies >Protein Purification & Characterization >YACs in Structural and Biological Genome Analysis >Yeast Genetics > >Courses in Neurobiology > >Brain Mapping >Developmental Neurobiology >Imaging Structure and Function in the Nervous System >Molecular Approaches to Ion Channel and Function >Molecular Cloning of Neural Genes >Neurobiology of Drosophila >Neurobiology of Human Neurological Disease: Mechanisms of Human Neurodegeneration >Structure, Function & Development of the Visual System >The Biology of Memory: From Molecules to Behavior > >4) Information for Course Applicants > >Registration: >Mail the application form plus 3 additional copies of application and accompanying material to the Course Registrar on or before the course deadline. If applying for more than one course, a separate application form plus 3 additional copies must be submitted for each course. Please select one course per session. > >Selection Process: >Each year, the instructors are confronted with the difficult task of selecting the students for our courses from a large number of well-qualified applicants. To facilitate the application process, students are asked to provide information documenting their request for a place in the course. First and foremost, applicants should give their reasons for wanting to take the course. As part of this, you should briefly outline your career. It is highly recommended that you supplement your application with one or more letters of recommendation. Applicants should remember that selection is based upon the degree to which they would benefit from this training opportunity; this in turn will be judged from the information given by the applicants. > >Applicants will be notified by mail whether or not they have been accepted to attend. The decisions often take several weeks after the application deadline to be finalized and announced. > >Stipends: > >Scholarship support is available to all qualified applicants based on need. Any candidate requesting financial assistance MUST request it when submitting the application. Attach a separate letter stating the amount requested and give a full justification for the required support. The amount awarded will be based upon the availability of funding. > >Housing: >All students must live in the Laboratory dormitories and take meals in the dining room. Most of the rooms are multiple occupancy and/or shared bath. Please be sure to indicate gender on the application form. Due to space limitations, families cannot be housed on grounds; there are no exceptions to this policy! > >Course Application Checklist: >Mail original plus 3 copies of the following: >** Original application for each course applied for. >** Letter and/or curriculum vitae outlining your career including reasons why taking the course would benefit you >** Letter(s) of recommendation >**(Stipend request) > >5) Prices for 1995 Courses: >Length of Course Cost * > >6 Days $1,155.00 >1 Week $1,215.00 >10 Days $1,460.00 >2 Weeks $1,720.00 <= COST OF THIS COURSE >3 Weeks $2,180.00 > >* Cost includes tuition, food and housing > >6) Sponsors of the 1994 Courses Program (not specifically for this course) > >The Howard Hughes Medical Institute ** The Esther A. & Joseph Klingenstein Fund, Inc. ** United States Department of Agriculture ** United States Department of Energy ** National Cancer Institute ** National Center of Human Genome Research ** National Institute of General Medical Sciences ** National Institute of Mental Health ** National Institutes of Health ** National Science Foundation > >7) Getting to Cold Spring Harbor Laboratory > >** New York Airports: J.F. Kennedy, La Guardia and L.I. MacArthur Airports are most convenient to the Laboratory (allow 1 1/2 - 2 hours travel time each way). Cold Spring Harbor Laboratory does not coordinate travel arrangements from local airports. >** Limousines: Prices for JFK and LaGuardia only! >Syosset Limousine - 516-364-9681, reservation required. Private Car (1 - 9 people). Cost = $65.00 for 1 person, $33.00 pp. for 2, $25.00 pp. for 3 or more. >Classic Limousine - 516-567-5100 (or 1-800-666-4949 outside New York). Shared Ride Service (possible stops at other terminals/destinations). Use courtesy phone at airport. No reservation required. Cost = $33.00 per person. >Other transportation options are available at the airports. Do not use taxicabs for transportation from the airport to CSHL. >** Railroad: Long Island Railroad is located in Penn Station. Take the PT JEFFERSON BRANCH train to SYOSSET. Note: Possible transfer at Jamaica Station. Taxis usually meet arriving trains at Syosset station ticket office. Syosset Taxi 921-2141; $6.50 for 1 person, $4.00 each additional person. >** Automobile: From the Long Island Expressway (Rte 495), >Exit 41 North (Route 106-107). On 106-107, bear right at fork (Route 106 - Oyster Bay). Travel 3.5 miles on Rte 106, make a right turn (East) onto Route 25A (town of East Norwich). Travel 4 miles. The lab entrance is on the left. (A gold Victorian house marks the entrance). >** New York - Connecticut Ferry Services: >Port Jefferson - Bridgeport (516-473-0286) >Orient Point - New London (516-323-2743) > >8) General Information about services at Cold Spring Harbor Laboratory > >** Registration: Please register at Grace Auditorium upon your arrival at the laboratory. Meetings begin at 7:30 in the evening. Dinner is served from 5:30 to 7:30 prior to meetings. Please call ahead if you plan to arrive at the lab after midnight. >** Parking: All vehicles MUST receive a Temporary Parking Permit from The Meetings Office. Any vehicle on grounds without such a permit will be subject to booting or towing. >** Phones / Faxes: Participants can receive faxes at the Meetings Office (516) 367-8845. Payphones and house phones are located throughout the campus. >Bookstore: The Bookstore is located in the lower level of Grace Auditorium. Store hours are posted. >** Computer Facilities: Lower level of Grace Auditorium, equipped with PC, Mac & laser printer. To access your e-mail, you must know the name of your home server. > >""""""""""""""""""""""""""""""""""""""""""""" >Correspondence should be addressed to: > >Cold Spring Harbor Laboratory >The Meetings Office >1 Bungtown Road, P.O. Box 100 >Cold Spring Harbor >New York 11724-2213 > >Phone: (meetings) 516 367 8346 > (courses) 516 367 8345 >Fax: 516 367 8845 >E-mail: meetings@cshl.org >World wide web site at http://www.cshl.org/ >""""""""""""""""""""""""""""""""""""""""""""" > I am looking for current research being done on breast cancer under human genome project. please reply to mmyers@ix.netcom.com From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news1.digex.net!access2.digex.net!pennie From: frog Newsgroups: bionet.genome.chromosomes Subject: Re: pcr taq pfu Date: Tue, 31 Jan 1995 15:23:39 -0500 Organization: Express Access Online Communications, USA Lines: 23 Message-ID: References: <9501262156.AA24623@dxi.nih.gov> NNTP-Posting-Host: access2.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <9501262156.AA24623@dxi.nih.gov> On 26 Jan 1995 goldman%bchem.dnet@DXI.NIH.GOV wrote: > Have tried pfu in straight pcr. it is a very unreliable and fussy enzyme, > but has the advantage of NO 3' transferase activity, and 3'-5' nuclease > activity for error correction. If you spike Taq with 1/100 pfu you keep the > high 'processiveness' of Taq and gain the editing function of pfu. > > ASHG > > > Would someone explain the kinetics of this to me. Surely you would need more than 1/100 (are we talking units here) in order to ensure taq wasn't proceding too far after an error before there was a chance of pfu getting on to proofread, or am I missing something (again). Bill pennieb@dce41.nci.nih pennie@access.digex.net From owner-chromosomes@net.bio.net Mon Jan 30 22:00:00 1995 Path: biosci!biosci!not-for-mail From: lstein@genome.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.genome.chromosomes,bionet.announce Subject: MIT CGR HUMAN PHYSICAL MAPPING DATA RELEASE 4 Date: 31 Jan 1995 15:13:37 -0800 Organization: Whitehead Institute for Biomedical Research Lines: 77 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <3gmfd7$8rs@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.genome.chromosomes:430 bionet.announce:1747 ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH HUMAN GENOMIC MAPPING PROJECT DATA RELEASE 4 (JANUARY 1995) The fourth release of data from the Human Physical Mapping Project at the Whitehead Institute/MIT Genome Center, covering data generated through the end of January, 1995, is now available. This data release contains YAC screening data for 5525 sequence tagged sites (STSs) screened on the CEPH mega-YAC library. For each STS, we report addresses for the YACs found to contain the STS. From the data obtained so far, there are over 900 contigs assembled using double linkage between STSs. The data is available electronically in two ways. ANONYMOUS FTP: The entire data release is available as a set of Microsoft Excel files and tab-delimited ascii files on our ftp server. Using an ftp client (such as "Fetch" on the Macintosh), connect to "ftp-genome.wi.mit.edu". Use "anonymous" as your user name, and give your e-mail address as your password. The data files are present in the directory /distribution/human_STS_releases/july94. The contents are as follows: 01-95.INTRO.mswd - Description of the data release, Macintosh format. 01-95.INTRO.txt - The same as ascii text 01-95.STS.YAC.sea.hqx - STS & YAC screening data, in MS-Excel format. 01-95.STS.YAC.txt - The same as tab-delimited text. 01-95.sequence - Full sequences of previously unpublished STSs. THE WORLD-WIDE WEB: You will need a World Wide Web client such as Mosaic (Unix, MS-Windows and Macintosh) or MacWeb (Macintosh). Instruct your client to connect to "http://www-genome.wi.mit.edu/". >From there, follow the "Human Physical Mapping Project" link. You will be able to browse and download the raw data set as well as to view doubly-linked contigs. A subset of the STSs (those for which we have chromosomal assignments) are also available through the Genome Database (GDB). QUESTIONS AND PROBLEMS. If users have any questions or problems, please contact us at human_STS_help@genome.wi.mit.edu We invite suggestions about how to make these data release most useful. DATA RELEASE POLICY AND CITATION. Data releases are scheduled every 90 days. At the end of each calendar quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within two weeks of the close of the quarter (i.e., in mid-January, mid-April, mid-July and mid-October). Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy aims to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. The information for the human genome mapping project should be cited as: Whitehead Institute/MIT Center for Genome Research, Human Physical Mapping Project, Data Release 4 (January 1995). -- ======================================================================== Lincoln D. Stein Whitehead Institute/MIT Genome Center lstein@genome.wi.mit.edu Cambridge, MA 02142 ========================================================================