From owner-chromosomes@net.bio.net Sun Jul 02 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!news.ksu.ksu.edu!usenet From: robs@ksu.ksu.edu Newsgroups: bionet.molbio.genome-program,bionet.genome.chromosomes,bionet.general Subject: Seeking Genome Size, Avg. Linker Length... Date: Mon, 03 Jul 95 13:17:39 PDT Organization: Kansas State University Lines: 37 Message-ID: NNTP-Posting-Host: s12.slip.ksu.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Xref: biosci bionet.molbio.genome-program:1434 bionet.genome.chromosomes:681 bionet.general:16015 I am looking for recent references or data on the 1.) Genome size 2.) Avg. length of linker segments connecting nucleosomes 3.) Cell cycle transit times, and 3.) Nuclear and cell volume of several species, i.e., E coli B/r, Saccharomyces cerevisiae (yeast), Neurospora crassa (fungas), Drosophila melanogaster, Lycopersicon esculentum (tomato), Mus musculus (mouse), and homo sapiens. I would also be very interested in a recent summary or review of these data that includes other species as well. The best I have been able to do so far is 1.) CRC handbook of Biochemistry: Selected Data for Molecular Biology circa 1968, 1970 2.) Cell Biology, ed. P.L. Altman and D.D. Katz, Federation of American Societies for Experimental Biology, Bethesda, Maryland circa 1976 3.) Cold Spring Harbor Symposia on Quantitative Biology Volume XLII (1978). 4.) Plus a couple of misc. articles dating back to the mid to late 70's and early 80's Any help would be greatly appreciated. Please reply by email, I will summarize if it seems appropriate. Rob Stewart Dept. of Nuclear Engineering Kansas State University Manhattan, KS 66502 robs@ksu.ksu.edu http://www.engg.ksu.edu/~robs/home.html From owner-chromosomes@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!hookup!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Genomic Imprinting Date: 5 Jul 1995 02:31:26 GMT Organization: McGill University Computing Centre Lines: 46 Message-ID: <3tcthu$ga1@sifon.cc.mcgill.ca> NNTP-Posting-Host: g-01.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) For all those of you interested in the phenomenon of genomic imprinting: It is NOT simply a gene dosage effect, and it is NOT only confined to the parent-of-origin-dependent expression of a gene. Although this is the functional end-point in certain cases (e.g. IGF2, H19, SNRPN and others), imprinting, in general, refers to the BEHAVIOR of a gene, depending on the sex of the parent which transmits it. This behavior can be in the form of replication asynchrony (e.g. igf2/H19 as well as a region within the Prader-Willi/Angelman syndrome locus), Differential methylation of chromosomal regions (either accompanied by parent-of-origin-dependent gene expression or not) and the severity/age-of-onset of certain human disorders. When refering to parent-of-origin gene expression and DNA methylation, imprinting has been shown to be a tissue-specific as well as a developmental-stage-specific phenomenon. It is also a polymorphic trait among individuals. Regarding X-chromosome inactivation, there are instances where a parent-of-origin-dependent inactivation occurs: this is in the extraembryonic tissues of mice, for example, and could conceivably occur due to a germline-dependent marking of the chromosome.. That certain diseases characterised by expanding trinucleotide repeats show an earlier age-of-onset and severity depending on the sex of the affected parent, in my opinion, is not a good example to define imprinting. Other mechanisms may be at play in these cases. The best examples, to date, are the Prader-Willi/Angelman Syndromes. They both map to the same chromosomal region (15q11-13), and both arise (usually) as a consequence of deletions within this region. When the deletion is of PATERNAL origin, the patient suffers from the Prader-Willi syndrome, a dysmorphic syndrome characterised by obesity, hypotonia and mental retardation. When the deletion is inherited from the MOTHER, it is the Angelman Syndrome which arises in the patient. Once again, this syndrome is characterised by mental retardation. This time, however, the patient is hyperactive and constantly laughs. Although the deletions occur in a common region, molecular studies indicate that the genes responsible are distinct. Indeed, there have been a number of genes cloned from the Prader-Willi critical region which are expressed only from the paternally-derived allele. No such genes have been cloned from the Angelman region, BUT, there is a small area, recently defined, that may harbor such genes. It is anticipated that this (these) genes will be expressed only from the maternally-derived allele. I hope to have enlightened you, but should you wish more information, I highly recommend a reading of Argiris Efstratiadis' review in Current Opinion in Genetics and Development (1994) 4:265-280, as well as Rob Nicholls' editorial in Am. J. Hum. Genet. (1994) 54:733-740. From owner-chromosomes@net.bio.net Tue Jul 04 23:00:00 1995 Path: biosci!rutgers!news.iag.net!usenet.eel.ufl.edu!noc.netcom.net!news.sprintlink.net!news.comp-unltd.com!server02 From: swest@imt.net Newsgroups: bionet.genome.chromosomes Subject: Genetics/Blood Type Date: Wed, 05 Jul 95 21:50:31 GMT Organization: Internet Montana Lines: 12 Message-ID: <3tf1f7$81s_001@server02.imt.net> NNTP-Posting-Host: server02.imt.net To: All X-Newsreader: News Xpress Version 1.0 Beta #3 My daughter-in-law has AB Pos blood - my son has AB Neg. She has suffered three near fatal miscarriages and has recently found out she is pregnant. The Doctor in the town through which she had the last miscarriage told them it could be a genetic chromosone problem...like on the "leg" was missing from the X or Y. Her mother is B neg and her father was O Pos. I am A Neg and took all the Rhogam shots many years ago. Could anyone offer some information to this problem? It would be most appreciated. Please understand it needs to be in layman's language. What genetic information from either side would be useful to us? Many thanks beforehand swest@imt.net From owner-chromosomes@net.bio.net Wed Jul 05 23:00:00 1995 Path: biosci!rutgers!gatech!paladin.american.edu!europa.chnt.gtegsc.com!library.ucla.edu!news.bc.net!rover.ucs.ualberta.ca!tribune.usask.ca!canopus.cc.umanitoba.ca!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re:Imprinting....elightenment Date: 6 Jul 1995 03:58:02 GMT Organization: McGill University (Exp. Medicine) Lines: 89 Message-ID: <3tfn0a$7pj@sifon.cc.mcgill.ca> NNTP-Posting-Host: o-07.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Nick wrote: >I hope to have enlightened you, but should you wish more information, I highly recommend a reading >of >Argiris Efstratiadis' review in Current Opinion in Genetics and Development (1994) 4:265-280, as well >as >Rob Nicholls' editorial in Am. J. Hum. Genet. (1994) 54:733-740. -------------------------------------------------------------------------------------------------------------------------- Thanks for the references... but the condescending tone whether ment directly or inavertently in "hope to enlighten you." seemed somewhat bitter for such an "elightened person as yourself. Benefit of the doubt is given. As per my first post prior to the 28th oif June which may be the first post you read Nick was in response to a Med student who wanted a quick and dirty definition of imprinting. I gave it to him. Gene dosage is an easy concept... replication timing as it pertains to disease is not. Praderwillie/Angleman disease is good example of imprinting at work in human disease but is far from it conceptual land mines and was therefore not a good start for someone. some Q.'s you wrote: >imprinting, in general, refers to the BEHAVIOR of a gene, depending on the sex of the parent >which transmits it. Behaviour is pretty ambiguous. You gave -replication timing -differential methylation -severity/age of onset of certain human disorders so gene expression(timing and tissue specificity) was ommited.... is this not a "behaviour" of a gene? hmmm. how is replication involved in disease? If it is in the reviews don't bother posting it, I will read it in its entirety rather than have you waste time paraphrasing it . okay differential methylation is the classic paradigm (perhaps involving replication timing too... there is work at McGill (in exp. Medicine actually in Maria Hadjopolos's (spell) lab I think) that has some interesting evidence that replication timing may involve some sort of methylation which is imprinted....EEK! excuse me if I lack the details it is late and I can't find my notes from the Exp. Med. Lecture Day . Aren't highly transcribed genes usually associated with early replicating DNA... therefore perhaps replication timing is a reflection of the transcription state of the DNA and then this of course may reflect on gene dosage again. I am in no way endorsing methylation as the imprinting "cure all" but it is a damn interesting phenomenon in itself. severity/age of onset... hmm this is multi-factorial I think (diabetes comes to mind) involving many genes and gene products. and ummm how does severity relate to imprinting ? Dosage may be involved conceivably couldn't it? Lots of interesting tidbits where clipped as they do not pertain to the discussion as to whether gene dosage is a good model to help someone "start" thinking about imprinting. I thought it was. I received a few complements from people apparently less "enlightened" for distilling some of the gobbly gook and disjointed thoughts down to a simple concept to hang on to. Imprinting is still a sticky subject! I give the benefit of the doubt to you Nick as I think you just read one of the later posts where we focussed on a small area of the imprinting phenomenon (ie. dosage as related to X inactivation and "Xist" and trinucleotide repeats of which a great review was sighted). You came in on a thread that is more than a week old. Graham P.S. Two bits appreciated quand meme : ) Good to see some more McGill people expressing themselves! _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Wed Jul 05 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!dish.news.pipex.net!pipex!uknet!bcc.ac.uk!news From: dcurtis@hgmp.mrc.ac.uk (David Curtis) Subject: Re: Genetics/Blood Type Message-ID: <1995Jul6.094845.36549@ucl.ac.uk> Date: Thu, 6 Jul 1995 09:48:39 GMT References: (none) <3tf1f7$81s_001@server02.imt.net> Organization: University College London X-Newsreader: Forte Free Agent v0.55 Lines: 35 swest@imt.net wrote: >My daughter-in-law has AB Pos blood - my son has AB Neg. She has suffered >three near fatal miscarriages and has recently found out she is pregnant. The >Doctor in the town through which she had the last miscarriage told them it >could be a genetic chromosone problem...like on the "leg" was missing from the > X or Y. Her mother is B neg and her father was O Pos. I am A Neg and >took all the Rhogam shots many years ago. Could anyone offer some information >to this problem? It would be most appreciated. Please understand it needs to >be in layman's language. What genetic information from either side would be >useful to us? It's hard to tell from your posting, but the blood group issue may be irrelevant. If the question is why she has had recurrent miscarriages, then the doctor may be suggesting that this could be because she or her husband has some chromosomal rearrangement that causes them no harm but which means that often the foetus conceived ends up with a very abnormal set of chromosomes which means that it cannot survive. If this has been the situation then subsequent pregnancies may be normal, but there may be a risk of another abnormal foetus and miscarriage. To diagnose this, a simple first step would be to examine the chromosomes of the parents (i.e. your son and daughter-in-law). If one of them does have such an abnormality (for example a "balanced translocation") then obtain a sample of cells from the foetus or placenta may then be indicated, to see if the foetus has ended up with a normal set of chomosomes or not. All this is very speculative. The real advice is: discuss the situation with the obstetrician and if indicated get appropriate genetic counselling and/or testing from a medical geneticist. Dave Curtis (dcurtis@hgmp.mrc.ac.uk) Institute of Psychiatry, London http://www.iop.bpmf.ac.uk/home/depts/psychmed/general/dcurtis/dcurtis.htm From owner-chromosomes@net.bio.net Wed Jul 05 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!EU.net!uknet!bcc.ac.uk!news From: dcurtis@hgmp.mrc.ac.uk (David Curtis) Subject: Re: Imprinting....elightenment Message-ID: <1995Jul6.095513.78747@ucl.ac.uk> Date: Thu, 6 Jul 1995 09:55:06 GMT References: (none) <3tfn0a$7pj@sifon.cc.mcgill.ca> Organization: University College London X-Newsreader: Forte Free Agent v0.55 Lines: 15 Ooh goody, a row in bionet.genome.chromosomes! Come on Nick, don't take it lying down. I'll post a general "Impending Flame War!" alert in a few other groups so that more people can come and watch. Yours in eager anticipation of some real metaphorical bloodshed, Dave Curtis (dcurtis@hgmp.mrc.ac.uk) Institute of Psychiatry, London http://www.iop.bpmf.ac.uk/home/depts/psychmed/general/dcurtis/dcurtis.htm From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (Helene Klapper ) Newsgroups: bionet.genome.chromosomes,bionet.molbio.evolution,bionet.molbio.genome-program,bionet.cellbio Subject: Re: Why are there 23 pairs of chromosomes? Date: 7 Jul 1995 23:01:28 GMT Organization: Netcom Lines: 25 Distribution: world Message-ID: <3tkec8$dls@ixnews6.ix.netcom.com> References: <3tk22o$d6e@news.ox.ac.uk> NNTP-Posting-Host: ix-mia1-28.ix.netcom.com Xref: biosci bionet.genome.chromosomes:691 bionet.molbio.evolution:3088 bionet.molbio.genome-program:1440 In <3tk22o$d6e@news.ox.ac.uk> Netscape User writes: > >Hi, > Why are there only 23 pairs of chromosomes in human while simpler creatures may >have many more chromosomes? What are the mechanisms that decide the number of >chromosomes in a creature? > All plausible suggestions will be much appreciated. > >best regards - teck sin > The size of the genome is probably more relevant than the number of chromosomes. Some organisms may have many, but they may be very small (such as avian microchromosomes). Certainly over time, evolution has contributed to the breaks of large xms into smaller ones, and the joining of smaller ones into larger ones. So, you could say that the xm number is always changing ( over millions of years, anyway). Besides, there is so much junk dna in the xms...the number of xms or size of the genome is not really indicative of the number of genes present...and the genes being present doesn't mean that they are active. I guess what I'm saying is that the number 23 is really meaningless - we could have many more or many less, and still have the same number of genes. From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Imprinting (sorry DaVE) Date: 7 Jul 1995 12:05:35 GMT Organization: McGill University (Exp. Medicine) Lines: 21 Message-ID: <3tj7uf$n1f@sifon.cc.mcgill.ca> NNTP-Posting-Host: a-12.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Kudos all around no flame war : ) Sorry Dave... G. _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!news.sprintlink.net!dish.news.pipex.net!pipex!uknet!bhamcs!news.ox.ac.uk!news From: Netscape User Newsgroups: bionet.genome.chromosomes,bionet.molbio.evolution,bionet.molbio.genome-program,bionet.cellbio Subject: Why are there 23 pairs of chromosomes? Date: 7 Jul 1995 19:31:36 GMT Organization: Pembroke College Lines: 8 Message-ID: <3tk22o$d6e@news.ox.ac.uk> NNTP-Posting-Host: eromanga.pmb.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Xref: biosci bionet.genome.chromosomes:689 bionet.molbio.evolution:3086 bionet.molbio.genome-program:1439 Hi, Why are there only 23 pairs of chromosomes in human while simpler creatures may have many more chromosomes? What are the mechanisms that decide the number of chromosomes in a creature? All plausible suggestions will be much appreciated. best regards - teck sin From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!news.sprintlink.net!dish.news.pipex.net!pipex!oleane!jussieu.fr!centre.univ-orleans.fr!univ-lyon1.fr!swidir.switch.ch!newsfeed.ACO.net!osiris.wu-wien.ac.at!i112syw1.vu-wien.ac.at!bunka From: bunka@i112syw1.vu-wien.ac.at (Sebastian Bunka) Newsgroups: bionet.genome.chromosomes Subject: Giemsa-11 species-spef. staining Date: 7 Jul 1995 13:19:21 GMT Organization: Vet. University Vienna Lines: 29 Distribution: world Message-ID: <3tjc8p$m02@osiris.wu-wien.ac.at> Reply-To: Sebastian.Bunka@vu-wien.ac.at NNTP-Posting-Host: i112syw1.vu-wien.ac.at X-Newsreader: slrn (0.7.3.0) Hi, anyone on the net that has done the Giemsa-11 technique for species specific chromosome differentiation ? We tried this for differentiation of Mouse-Cat heterohybridomas, but the stain does'nt work for us. Any ideas or comments ? From the original paper: C. Buys, G. Aanstoot, and A. Nienhaus, 1984, Histochem. 81:465-468: "Oxidizing Methylene blue and adding the reaction products to Eosin Y and Azure B makes possible a highly reliable Giemsa-11 technique for discrimination of chromosomes in hybrid cells according to their parental origin..." Thanks in advance for any comments and tips!! Cheers, Sebastian -- ;-) ------------------------------------------------------------------------ Sebastian Bunka ph. (+43-1) 711 55 301 Institute of FAX (+43-1) 713 68 95 Medical Chemistry email: Sebastian.Bunka@vu-wien.ac.at University of Veterinary Medicine - Vienna - Austria ------------------------------------------------------------------------ From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!hookup!news.mcgill.ca!o-02.das.mcgill.ca!user From: b2d6@musicb.mcgill.ca (Nick Giannoukakis) Newsgroups: bionet.genome.chromosomes Subject: Re: Imprinting....elightenment Date: 6 Jul 1995 15:55:14 GMT Organization: McGill University Lines: 185 Message-ID: References: <3tfn0a$7pj@sifon.cc.mcgill.ca> NNTP-Posting-Host: o-02.das.mcgill.ca Hello Graham, First of all, yes I did come in late on the thread of the discussion. Second, I did not wish to be condescending in any manner, my posting was in no way designed to flame. Being very intimate with the field, I felt that some clarification needed to be made. Your example about gene dosage is appropriate to explain the phenomenon at the functional level, but we are not sure that this is always the case. My apologies to those who may have been offended. The term "behavior" is not ambiguous. In fact it encompasses all the possible parent-of-origin-dependent phenomena. I feel that it is a more appropriate term to use, rather than referring to gene expression, which, as I indicated in my previous post, is in certain cases, the functional end-point. We cannot assume that EVERY imprinted gene will act this way, at the transcriptional level. In fact, diabetes (type I) is transmitted preferentially from fathers to affected offspring, and two of the recently mapped susceptibility loci contain imprinted genes (11p15 and 6q25-27; IGF2 and IGF2R, fascinating, no?). How imprinted genes affect the age-at-onset and severity of certain diseases is at present not known, so any hypotheses would be welcome. You are right in surmising that the expression levels of the genes involved would play a role, but, again, this would be the functional end-point. Something must have gone wrong either at the stage where the imprint was established, in the interpretation of the imprint, functionally, post-fertilisation, or both. Regarding replication asynchrony, instead of me wasting bandwidth, Nicholls' review should be a starting point to answer your question. And, yes, I wholeheartedly agree that McGill should dominate the Internet! Now, what about those impending fees........ Nick. In article <3tfn0a$7pj@sifon.cc.mcgill.ca>, Graham Dellaire wrote: > Nick wrote: > > >I hope to have enlightened you, but should you wish more information, I highly recommend a reading > >of > >Argiris Efstratiadis' review in Current Opinion in Genetics and Development (1994) 4:265-280, as well > >as > >Rob Nicholls' editorial in Am. J. Hum. Genet. (1994) 54:733-740. > > -------------------------------------------------------------------------------------------------------------------------- > > Thanks for the references... but the condescending tone whether ment directly or > inavertently in "hope to enlighten you." seemed somewhat bitter for such an "elightened > person as yourself. > > Benefit of the doubt is given. > > As per my first post prior to the 28th oif June which may be the first post you > read Nick was in response to a Med student who wanted a quick and dirty > definition of imprinting. I gave it to him. Gene dosage is an easy concept... > replication timing as it pertains to disease is not. Praderwillie/Angleman disease > is good example of imprinting at work in human disease but is far from it conceptual > land mines and was therefore not a good start for someone. > > some Q.'s > > you wrote: > >imprinting, in general, refers to the BEHAVIOR of a gene, depending on the sex of the parent > >which transmits it. > > Behaviour is pretty ambiguous. > You gave > -replication timing > -differential methylation > -severity/age of onset of certain human disorders > > so gene expression(timing and tissue specificity) was ommited.... is this not a "behaviour" of a gene? > > hmmm. how is replication involved in disease? If it is in the reviews don't bother posting it, I will > read it in its entirety rather than have you waste time paraphrasing it . > > okay differential methylation is the classic paradigm (perhaps involving replication timing too... > there is work at McGill (in exp. Medicine actually in Maria Hadjopolos's (spell) lab I think) that has > some interesting evidence that replication timing may involve some sort of methylation which is > imprinted....EEK! excuse me if I lack the details it is late and I can't find my notes from the > Exp. Med. Lecture Day . Aren't highly transcribed genes usually associated with early > replicating DNA... therefore perhaps replication timing is a reflection of the transcription state > of the DNA and then this of course may reflect on gene dosage again. > > I am in no way endorsing methylation as the imprinting "cure all" but it is a damn interesting > phenomenon in itself. > > severity/age of onset... hmm this is multi-factorial I think (diabetes comes to mind) involving many > genes and gene products. and ummm how does severity relate to imprinting ? Dosage may be > involved conceivably couldn't it? > > > Lots of interesting tidbits where clipped as they do not pertain to the discussion as to > whether gene dosage is a good model to help someone "start" thinking about imprinting. > I thought it was. I received a few complements from people apparently less "enlightened" > for distilling some of the gobbly gook and disjointed thoughts down to a simple concept to > hang on to. > > Imprinting is still a sticky subject! > > I give the benefit of the doubt to you Nick as I think you just read one of the later posts > where we focussed on a small area of the imprinting phenomenon (ie. dosage as related to X > inactivation and "Xist" and trinucleotide repeats of which a great review was sighted). > > You came in on a thread > that is more than a week old. > > Graham > > P.S. Two bits appreciated quand meme : ) > Good to see some more McGill people expressing themselves! > _______________________________________________________________________ > Graham Dellaire Snail Mail: > Red Cross, Research > McGill University Montreal Blood Services > Faculty of Medicine 3131 Sherbrooke St. East > Div. of Experimental Medicine Montreal, QC, Canada > E-mail: popa0206@po-box.mcgill.ca H1W 1B2 > B2XE@musicb.mcgill.ca > WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html > Fax: (514) 525 0881 > Voice: (514) 527 1501 ext 175 > _______________________________________________________________________ From owner-chromosomes@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (Helene Klapper ) Newsgroups: bionet.genome.chromosomes Subject: Re: Giemsa-11 species-spef. staining Date: 7 Jul 1995 22:52:30 GMT Organization: Netcom Lines: 48 Distribution: world Message-ID: <3tkdre$dhi@ixnews6.ix.netcom.com> References: <3tjc8p$m02@osiris.wu-wien.ac.at> NNTP-Posting-Host: ix-mia1-28.ix.netcom.com In <3tjc8p$m02@osiris.wu-wien.ac.at> bunka@i112syw1.vu-wien.ac.at (Sebastian Bunka) writes: > > >Hi, >anyone on the net that has done the Giemsa-11 technique for species specific >chromosome differentiation ? > >We tried this for differentiation of Mouse-Cat heterohybridomas, but the >stain does'nt work for us. Any ideas or comments ? > >From the original paper: >C. Buys, G. Aanstoot, and A. Nienhaus, 1984, Histochem. 81:465-468: > >"Oxidizing Methylene blue and adding the reaction products to Eosin Y and >Azure B makes possible a highly reliable Giemsa-11 technique for >discrimination of chromosomes in hybrid cells according to their parental >origin..." > >Thanks in advance for any comments and tips!! > >Cheers, Sebastian >-- > >;-) >---------------------------------------------------------------------- - >Sebastian Bunka ph. (+43-1) 711 55 301 >Institute of FAX (+43-1) 713 68 95 >Medical Chemistry email: Sebastian.Bunka@vu-wien.ac.at >University of Veterinary Medicine - Vienna - Austria >---------------------------------------------------------------------- - > I have several protocols for this, each somewhat different than the others, but I have gotten none of them to work. A friend of mine who is an expert in such matters tells me that the pH is CRITICAL - that it has to be exact, and that it is temperature dependent. Most of my protocols involve doing it in a waterbath (above room temp - I don't remember if it's 37 degrees or what...my notes are in the lab and I am at home) so she has told me that I need to take the pH of the soln while it is in the waterbath. If you take it out to pH it, the temp changes and therefore, the pH is no longer accurate. I hope this helps somewhat. Helene From owner-chromosomes@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!UQTR.UQuebec.ca!gabashvi From: gabashvi@UQTR.UQuebec.ca (Irene Gabashvili) Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics/Blood Type Date: 8 Jul 1995 15:17:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net From one of the last msgs posted to CHROMOSOMES group: > My daughter-in-law has AB Pos blood ... > Her mother is B neg and her father was O Pos... Sorry to be so sucpicious. Is it possible? B group means BB or BO genotype whereas only OO genotype can give O group (according to all Biology textbooks) Children of B neg and O pos can have only B (BO) or O group (both pos or neg). Would be glad if somebody prove that I am mistaken. Sincerely, Irene Gabashvili From owner-chromosomes@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 7 Jul 1995 21:26:14 GMT Organization: McGill University (Exp. Medicine) Lines: 75 Message-ID: <3tk8pm$f8c@sifon.cc.mcgill.ca> References: <3tk22o$d6e@news.ox.ac.uk> NNTP-Posting-Host: u-12.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) > Netscape User writes: > Hi, > Why are there only 23 pairs of chromosomes in human while simpler creatures may > have many more chromosomes? What are the mechanisms that decide the number of > chromosomes in a creature? > All plausible suggestions will be much appreciated. > > best regards - teck sin > > >>>> First of all there is no real correlation between the complexity of an organism and the number of chromosomes. We have 23 pairs and an animal much like a deer called a "Mutjak" only has four pairs (all be it very large ones). Now can you say a the Mutjak is more or less complex then ourselves.... probably not . Plants are notorious for having huge numbers of chromosomes in comparison to mammals. My guess is the number of chromosomes we have is partly by chance. I think that we may never really be able to predict the original number of chromosomes that a mammalian "progenitor" species had. Some possible limiting factors for genome size may include: 1. Replication of entire genome during one cell cycle a)-it is conceivable that beyond a certain limit the cell would have such a large genome it could not replicate it entirely during one cells cycle and the cell would die. 2. Minimum size of linear chromosome able to replicate a) there is probably a minimum size for a chromosome.... such that it would contain the necessary DNA structures (ORI/ORC's etc) for replication. Mammalian cells are much more complicated than bacteria where the region required for efficient replication is very small (0.5-1 kb)... whereas in mammalian cells this region may invlove Mb's of DNA. 3. Robertsonian Translocations/chromosome fusions a) there is evidence that many of our metacentric chromosomes may actually be derived from two or more acrocentric chromosomes (evidence from In situ hybridisation to acrocentric chromosomes of the mouse ... I think Chms 17 and 11 of the mouse are good examples) Therefore it is possible the number of chromosomes we have could increase or decrease through such a mechanism as fusion of centromeres and the accompanying rearrangements. 4. Also the amount of non coding DNA tolerated (perhaps required) by an organism may increase the overall genome size. In yeast there are very few introns and bacteria lack them all together (I believe, though recent studies may have found a few they are definately rare). Therefore to have the required number of genes for the organism you need less DNA. Following this we would require more DNA partly because of introns and other noncoding regions of the genome and partly because of an increase "complexity" inherent in being mutlitcellular. Well that is a few things that come to me right away if I think of any more I will try to post Hopefully you get a few posts and between all of them there should be a few nuggets of (potential) truth! G _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!news.bc.net!rover.ucs.ualberta.ca!hookup!nstn.ns.ca!newsflash.concordia.ca!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 8 Jul 1995 02:13:30 GMT Organization: McGill University Computing Centre Lines: 11 Message-ID: <3tkpka$7fv@sifon.cc.mcgill.ca> NNTP-Posting-Host: s-01.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Although this is a very hard question to answer, as no solid (or any!) correlations exist between genome size and organism complexity, my speculation, at this moment, is that certain organisms may live in an environment that is favorable for high mutation rates. As such, a large genome, containing many non-coding regions, would serve as a buffer against deleterious mutations. Call it a sort of "sink" for mutations. I really can't think of anything else. This question reminds me of the phenomenon where small organisms in size, make a lot of Growth Hormone (those that produce it, anyway). Again, no explanations........ Nick. From owner-chromosomes@net.bio.net Fri Jul 07 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Flame War/Dave Curtis Date: 7 Jul 1995 12:42:48 GMT Organization: McGill University Computing Centre Lines: 13 Message-ID: <3tja48$6o@sifon.cc.mcgill.ca> NNTP-Posting-Host: u-04.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Dear Dave, First of all, this is in no way a flame war, and it would be greatly appreciated if you contributed to the scientific discussions of interest to the newsgroup, where all of us could benefit. Rumors like this will not do justice to the group, its subscribers or to the Internet community as a whole. Tell you what, why don't you come over and watch Graham and I challenge each other over a good game of Doom II ? All the best, Nick. From owner-chromosomes@net.bio.net Sat Jul 08 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (Helene Klapper ) Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics/Blood Type Date: 9 Jul 1995 21:55:08 GMT Organization: Netcom Lines: 24 Distribution: world Message-ID: <3tpj7s$ccr@ixnews4.ix.netcom.com> References: NNTP-Posting-Host: ix-mia2-03.ix.netcom.com In gabashvi@UQTR.UQuebec.ca (Irene Gabashvili) writes: > > >From one of the last msgs posted to CHROMOSOMES group: > >> My daughter-in-law has AB Pos blood ... >> Her mother is B neg and her father was O Pos... > >Sorry to be so sucpicious. > >Is it possible? > >B group means BB or BO genotype whereas only OO genotype >can give O group (according to all Biology textbooks) >Children of B neg and O pos can have only B (BO) or O >group (both pos or neg). > >Would be glad if somebody prove that I am mistaken. > >Sincerely, >Irene Gabashvili You're absolutely correct - I suppose someone's been lying to the poor girl about who her father is... From owner-chromosomes@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!dish.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!hgmp.mrc.ac.uk!mbishop From: mbishop@hgmp.mrc.ac.uk (Martin Bishop) Newsgroups: bionet.genome.chromosomes,bionet.molbio.evolution,bionet.molbio.genome-program,bionet.cellbio Subject: Re: Why are there 23 pairs of chromosomes? Date: 10 Jul 1995 11:51:47 GMT Organization: UK HGMP Resource Centre Lines: 8 Distribution: world Message-ID: <3tr48j$go3@mercury.hgmp.mrc.ac.uk> References: <3tk22o$d6e@news.ox.ac.uk> <3tkec8$dls@ixnews6.ix.netcom.com> NNTP-Posting-Host: osmium.hgmp.mrc.ac.uk Xref: biosci bionet.genome.chromosomes:700 bionet.molbio.evolution:3097 bionet.molbio.genome-program:1443 The classic case for believing that the number of chromosomes is irrelevant is the muntjac deer where almost identical species have ? 5 and ? 40 chromosomes (dont have a book to hand with the precise figures). Chromosomes organise the DNA and allow meiosis and mitosis. The specific number appears to be unimportant. Martin Bishop From owner-chromosomes@net.bio.net Sun Jul 09 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bloom-beacon.mit.edu!gatech!news.sprintlink.net!dish.news.pipex.net!pipex!uknet!bcc.ac.uk!news From: dcurtis@hgmp.mrc.ac.uk (David Curtis) Subject: Re: Flame War/Dave Curtis Message-ID: <1995Jul10.095444.76340@ucl.ac.uk> Date: Mon, 10 Jul 1995 09:54:24 GMT References: (none) <3tja48$6o@sifon.cc.mcgill.ca> Organization: University College London X-Newsreader: Forte Free Agent v0.55 Lines: 18 Nick Giannoukakis wrote: >Tell you what, why don't you come over and watch Graham and I challenge each other over a good >game of Doom II ? It's a bit of way for me to come. Can McGill contribute to my travel expenses? Alternatively would it be possible to set up some kind of WWW server to display the course of the game in real time? In my experience there are very few scientific disagreements which are not best settled mano a mano, though Descent is better for this than Doom II as it demands the ability to coordinate multiple-modality attacks while flying backwards in a helix to avoid incoming homing missiles, all the while taking care not to crash into hot lava. Dave Curtis (dcurtis@hgmp.mrc.ac.uk) Institute of Psychiatry, London http://www.iop.bpmf.ac.uk/home/depts/psychmed/general/dcurtis/dcurtis.htm From owner-chromosomes@net.bio.net Sun Jul 09 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bloom-beacon.mit.edu!gatech!news.sprintlink.net!dish.news.pipex.net!pipex!uknet!bcc.ac.uk!news From: dcurtis@hgmp.mrc.ac.uk (David Curtis) Subject: Re: Why are there 23 pairs of chromosomes? Message-ID: <1995Jul10.101214.57320@ucl.ac.uk> Date: Mon, 10 Jul 1995 10:11:54 GMT References: (none) <3tk22o$d6e@news.ox.ac.uk> Organization: University College London X-Newsreader: Forte Free Agent v0.55 Lines: 25 Since I'm being encouraged to increase the intellectual content of this group, I'll make the following contribution. As pointed out elsewhere, the number of chromosomes has little relation to the number of genes. All animals share common ancestry, so the fact that nowadays they have different numbers of chromosomes means that at some point in evolution the number changed from parent to child through trisomies, fusions and other rearrangements. In fact, these events have carried on occurring until very recently in evolutionary terms, and presumably may continue to do so. Except that for humans almost all trisomies are lethal and certainly compromise the capacity to reproduce dramatically. Are all tetrasomies lethal? I would expect so. If so, it would seem likely that humans will be "stuck" with 23 chromosomes indefinitely. Would most people agree? How about other species? Is there any remaining flexibility in their chromosomal complement? On a tangentially related point, we know that the genome size depends largely on how much junk DNA is around, but can anybody explain _why_ the puffer fish has so little junk DNA? Dave Curtis (dcurtis@hgmp.mrc.ac.uk) Institute of Psychiatry, London http://www.iop.bpmf.ac.uk/home/depts/psychmed/general/dcurtis/dcurtis.htm From owner-chromosomes@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 10 Jul 1995 13:05:42 GMT Organization: McGill University (Exp. Medicine) Lines: 88 Message-ID: <3tr8j6$8rh@sifon.cc.mcgill.ca> References: <1995Jul10.101214.57320@ucl.ac.uk> NNTP-Posting-Host: a-07.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) > dcurtis@hgmp.mrc.ac.uk (David Curtis) writes: > Since I'm being encouraged to increase the intellectual content of > this group, I'll make the following contribution. > > As pointed out elsewhere, the number of chromosomes has little > relation to the number of genes. All animals share common ancestry, so > the fact that nowadays they have different numbers of chromosomes > means that at some point in evolution the number changed from parent > to child through trisomies, fusions and other rearrangements. I have wrestled with this line of thinking for a while now. It is more likely as you describe here that the change in number of chromosomes was in smaller increments than say whole genome duplications as has been hypothesized to explain comparative mapping between species and within species. It would seem highly unlikely that "gross" rearrangements if not lethal in there own right would then be heritable. How would you find a another memeber of your "new species" with a similarly amplified and rearranged genome? You would be sterile for all intents and purposes. One mechanism that can drive changes and provide amplified regions of DNA .... and thus provide regions for comparative mappers to find is gene conversion between repeat like elements. This process has very recently been shown to occur at a very high rate! At such a rate that most probably the majority of our cells are actually genetic mosaics. ( I will try to find out the a reference... it has to do with studies of Line-1 sequences and there movement in embryonic cells) As well gene conversion has been hypothesized to been involved in the homology search at meiosis before synapsis and during. In fact, > these events have carried on occurring until very recently in > evolutionary terms, and presumably may continue to do so. Except that > for humans almost all trisomies are lethal and certainly compromise > the capacity to reproduce dramatically. Are all tetrasomies lethal? I wonder how any complex organism can tolerate tri or tetrasomies... I guess it depends on what genes are duplicated and how bad the ensuing rearrangements will be after duplication. Q. Are people with Downs Syndrome partially or totally sterile? > I would expect so. If so, it would seem likely that humans will be > "stuck" with 23 chromosomes indefinitely. Would most people agree? Hmm on the surface perhaps yes. But in the end who knows... "Nature" has its surprises... the post with about the Mutjac's one with 4 and the other with 40 chromosomes is interesting as they are supposedly quite similar. Maybe there are some humans in the Ozarks (spell?) of Arkansaw with more or less than the normal number of chromosomes. > On a tangentially related point, we know that the genome size depends > largely on how much junk DNA is around, but can anybody explain _why_ > the puffer fish has so little junk DNA? Hmm. How about this. Perhaps the fish arose from a progenitor species that could not tolerate retroviral or retroposon like invasion of its genome.... consequently you have very little repeats and intron-like "junk". I am out on a limb here completely as I have little experience with fish genomes in general....are they all largely junk free? or is the puffer a real exception to the rule. Graham > Dave Curtis (dcurtis@hgmp.mrc.ac.uk) > Institute of Psychiatry, London > http://www.iop.bpmf.ac.uk/home/depts/psychmed/general/dcurtis/dcurtis.htm > > >>>> _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Sun Jul 09 23:00:00 1995 Path: biosci!GDB.ORG!rrobbins From: rrobbins@GDB.ORG (Robert Robbins) Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 10 Jul 1995 04:59:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 103 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <1995Jul10.101214.57320@ucl.ac.uk> NNTP-Posting-Host: net.bio.net On Mon, 10 Jul 1995, David Curtis wrote: > If so, it would seem likely that humans will be > "stuck" with 23 chromosomes indefinitely. Would most people agree? How > about other species? Is there any remaining flexibility in their > chromosomal complement? Humans are no more "stuck" at the moment than they ever have been. Viable chromosomal re-arrangements are rare at the level of individuals, but not so rare at the level of evolutionary time. A comparison of the karyotypes of humans, gorillas, orangutans, and chimpanzees shows that some changes have occurred during the time that these four species diverged. Of course, that's over perhaps 10 million years and humans have been "humans" (in the sense of possessing written language, significant culture, etc.) for less than 10,000 years. Indeed, the duration to date of "human" existence on the planet is so short, compared with other biological processes, that it can barely be characterized as anything other than a momentary aberration. Some momentary aberrations prove to be durable -- that's why we consider archaeopteryx to be the first bird and not just a unique, funny-looking lizard. > On a tangentially related point, we know that the genome size depends > largely on how much junk DNA is around, but can anybody explain _why_ > the puffer fish has so little junk DNA? Because all statistical distributions have tails, is why. If we assume that the accumulation, or loss, of "junk" DNA has a random component, than the distribution of junk DNA across different taxa will be governed, in part, by some statistical distribution. Now, suppose I throw 30 coins in the air, then score the result in terms in the numbers of heads and tails that appear. The odds that they will all come up heads is vanishingly small -- on the order of one out of a billion. But, if I repeat my toss several billion times, a few of them will almost come up all heads. Asking why those particular tosses came up all heads would have a lot in common with asking why a puffer fish has more junk DNA than other critters. It may be that there are some non-random processes associated with the accumulation and distribution of junk DNA, and if that's the case, then there might be an answer to the why question. But we do have reason to believe that at least some of the processes are governed by chance, and given that, it's useful to remember that "all statistical distributions have tails." It's also useful to remember that every possible INDIVIDUAL outcome of my 30-coin toss has the same probability of occurring (one in a billion) as every other one. That is, suppose my 30 coins were all different - one penny, one nickel, one pfennig, one mark, etc. - so that each toss could be recorded as penny:heads, nickel:tails, pfennig:tails; mark:tails, etc. Scored that way, there are about one billion different, individual outcomes, each one equally likely. Of those billion outcomes, there is only one way to get all heads (where # = heads and - = tails): ############################## but 30 different ways to get one tail and 29 heads: -############################# #-############################ ##-########################### ###-########################## ####-######################### #####-######################## ######-####################### #######-###################### ########-##################### #########-#################### ##########-################### ###########-################## ############-################# #############-################ ##############-############### ###############-############## ################-############# #################-############ ##################-########### ###################-########## ####################-######### #####################-######## ######################-####### #######################-###### ########################-##### #########################-#### ##########################-### ###########################-## ############################-# #############################- and 30 x 29 ways to get 28 heads and 2 tails, etc etc. The "fact" that a 50:50 outcome of heads and tails is expected does not mean that any PARTICULAR 50:50 outcome is especially likely (indeed, each one is just as unlikely as all heads) -- it just means that there are so many different ones that could happen. Bottom line: one should not confuse the results of statistical processes with the results of causal processes... From owner-chromosomes@net.bio.net Mon Jul 10 23:00:00 1995 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!xlink.net!rz.uni-karlsruhe.de!news.uni-stuttgart.de!news.belwue.de!fu-berlin.de!rldb-general.rz-berlin.mpg.DE!not-for-mail From: mercer@mpimg-berlin-dahlem.mpg.de (RLDB) Newsgroups: bionet.genome.chromosomes Subject: WARNING (Was: Genetics/Blood Type) Date: 11 Jul 1995 15:20:43 GMT Organization: RLDB Lines: 30 Sender: -Not-Authenticated-[3949] Distribution: world Message-ID: <3tu4sb$2a4@fu-berlin.de> References: <3tpj7s$ccr@ixnews4.ix.netcom.com> Reply-To: mercer@mpimg-berlin-dahlem.mpg.de NNTP-Posting-Host: rldb-general.rz-berlin.mpg.de (141.14.130.44) X-Access: 16 25 816 X-Posted-From: InterNews 1.0.6@rldb-general.rz-berlin.mpg.de Xdisclaimer: No attempt was made to authenticate the sender's name. Dear users of this newsgroup, 1) I know you are only trying to do good 2) I am not trying to start a flame war 3) and who am I to tell you how to behave anyway? ... but if I might make a suggestion anyway... I have been reading this newsgroup for several years, and every so often some layperson writes in to find out more about a medical problem they have, or which affects someone they know.The first message in this thread is a prime example. Please PLEASE be careful how you answer! If your are not a qualified medic, or qualified in patient counselling and are prepared to continue to support them after you casually drop some bombshell into their lives, do not answer them! This is not the most charitable way to behave I know, but this is not a game or an intellectual excercise. To give an example, this thread might well have screwed up the lives of some family somewhere. Please be careful. If you have to flame me, reply to mercer@mpimg-berlin-dahlem.mpg.de and keep it out of the group. Simon From owner-chromosomes@net.bio.net Tue Jul 11 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bloom-beacon.mit.edu!news.moneng.mei.com!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!acer.gen.tcd.ie!dbarton From: dbarton@acer.gen.tcd.ie (Dr David E Barton) Subject: Re: WARNING (Was: Genetics/Blood Type) Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Irish National Centre for Medical Genetics References: Date: Wed, 12 Jul 1995 11:10:14 GMT Lines: 37 In article <3tu4sb$2a4@fu-berlin.de>, RLDB wrote: > >Dear users of this newsgroup, > > 1) I know you are only trying to do good > 2) I am not trying to start a flame war > 3) and who am I to tell you how to behave anyway? > >... but if I might make a suggestion anyway... > >I have been reading this newsgroup for several years, and every so >often >some layperson writes in to find out more about a medical problem they >have, or which affects someone they know.The first message in this thread >is a prime example. Please PLEASE be careful how you answer! If you are >not a qualified medic, or qualified in patient counselling and are >prepared to continue to support them after you casually drop some >bombshell into their lives, do not answer them! (snip) I couldn't agree more. I reply to such queries by e-mail, giving the "patient" details of their nearest medical genetics centre (from the ASHG directory), AND advising them to ignore any medical advice they get direct over the internet. It's often difficult to resist giving people advice when the question SEEMS straightforward, but resist we must! David. | David Barton | National Centre for Medical Genetics | Our Lady's Hospital for Sick Children | Crumlin, Dublin 12, Ireland. | Tel +353 1 455 0515 Fax +353 1 455 8873 From owner-chromosomes@net.bio.net Wed Jul 12 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!dish.news.pipex.net!pipex!warwick!yama.mcc.ac.uk!news.york.ac.uk!news From: "M.E. Holt" Newsgroups: bionet.genome.chromosomes Subject: T. Annulata Cloning Project... Date: 13 Jul 1995 13:34:22 GMT Organization: The University of York, UK Lines: 18 Message-ID: <3u37cu$d23@mailer.york.ac.uk> NNTP-Posting-Host: sgi14.york.ac.uk Hi, I am about to embark on an attempt to clone the 5' end of the actin gene from the Theileria Annulata genome. Is anyone out there aware of any research that may have been done in this field before? All information gratefully accepted. M.E. Holt (meh100@tower.york.ac.uk) _____________________________________ /____________________________________/ / \_University of York,________________\ /_Department of Biological Sciences,_/ \_Heslingon__________________________\ /_York,______________________________/ \_YO1 5DD____________________________\ /____________________________________/ From owner-chromosomes@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!gatech!cs.utk.edu!stc06.ctd.ornl.gov!jkm.ppp.ornl.gov!user From: jkm@ornl.gov (John K. Munro, Jr.) Newsgroups: bionet.genome.chromosomes Subject: Genetics-Related Disease States Date: 13 Jul 1995 22:42:57 GMT Organization: Oak Ridge National Laboratory Lines: 14 Message-ID: NNTP-Posting-Host: jkm.ppp.ornl.gov While the Human Genome Database at Johns Hopkins University contains information on genetics-related disease states for each chromosome, this information is not convenient to locate and retrieve, i.e. through any text search capability. I would like references on the network (preferably Web or WAIS servers) to easily and quickly text searchable databases of this information. For example, I want to search for "diabetes" or "Down's Syndrome" and get a list of chromosomes related to these disease states. John K Munro Jr Oak Ridge National Laboratory jkm@ornl.gov (615) 574-0635 FAX: (615) 576-8380 From owner-chromosomes@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!not-for-mail From: christy.davis@srs.gov (Christy Davis) Newsgroups: bionet.genome.chromosomes Subject: Novice question Date: 14 Jul 1995 14:10:28 -0500 Organization: WSRC Lines: 2 Sender: nobody@cs.utexas.edu Message-ID: <01HSV52NO5O20011QU@Mailhub.srs.gov> NNTP-Posting-Host: news.cs.utexas.edu Can someone please explain to me (in simple terms) how eye color in humans is determined? Thanks. From owner-chromosomes@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!gatech!swrinde!howland.reston.ans.net!dish.news.pipex.net!pipex!tank.news.pipex.net!pipex!lyra.csx.cam.ac.uk!news From: Andrew Palin Newsgroups: bionet.genome.chromosomes Subject: Cytogenetics newsgroup Date: 14 Jul 1995 15:45:22 GMT Organization: University of Cambridge, England Lines: 3 Message-ID: <3u63ei$a0u@lyra.csx.cam.ac.uk> NNTP-Posting-Host: png-mac9.gen.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:bionet.genome.chromosomes Does anyone know of a cytogenetics\FISH nwsgroup From owner-chromosomes@net.bio.net Thu Jul 13 23:00:00 1995 Path: biosci!GDB.ORG!rrobbins From: rrobbins@GDB.ORG (Robert Robbins) Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics-Related Disease States Date: 14 Jul 1995 13:10:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Have you tried http://gdbwww.gdb.org/omim/omimq This provides a text query into OMIM, a full text database of information on human geneics and disorders. The way you have phrased your potential questions, "what chromosomes are related to disease state X," is a little problematic, since the answer in nearly every case will depend on just what you mean by "related." Although Down's Syndrome, for example, is associated having three copies of chromosome 21, the phenotype is caused not by the actual presence of the third copy of an otherwise normal chromosome 21 but rather by the imbalance in the ratio of the dosage of chromosome 21 to the other chromosomes, so in a loose sense of "related" Down's syndrome is related to all chromosomes, but appears to result from three copies of chromosome 21 in an otherwise normal set of chromosomes. As for diabetes, a full text search on that word into OMIM produced a large set of 212 "hits." Reading through all of them would give a lot of candidate chromosomes that might be "related" in one way or another with diabetes. On 13 Jul 1995 jkm@ornl.gov wrote: > While the Human Genome Database at Johns Hopkins University contains > information on genetics-related disease states for each chromosome, this > information is not convenient to locate and retrieve, i.e. through any > text search capability. I would like references on the network > (preferably Web or WAIS servers) to easily and quickly text searchable > databases of this > information. For example, I want to search for "diabetes" or "Down's > Syndrome" and get a list of chromosomes related to these disease states. > > John K Munro Jr > Oak Ridge National Laboratory > jkm@ornl.gov > (615) 574-0635 > FAX: (615) 576-8380 > From owner-chromosomes@net.bio.net Fri Jul 14 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 15 Jul 1995 00:55:33 GMT Organization: University of Washington, Seattle Lines: 58 Message-ID: <3u73m5$5pc@nntp3.u.washington.edu> References: <1995Jul10.101214.57320@ucl.ac.uk> <3tr8j6$8rh@sifon.cc.mcgill.ca> NNTP-Posting-Host: saul2.u.washington.edu NNTP-Posting-User: roach I thought I might add a couple comments. 1) The evidence is overwhelming that there have been several independent tetraploidizations during vertebrate evolution. Most of the interesting papers (with the hardest data) come from the Salmonid geneticists; look in Medline for these. Also, up my own alley, the vertebrate immune system clearly arose in its present form following a tetraploidization. It is the simplest and perhaps only reasonable explanation for the parallel genomic structure of the heavy and light immunoglobulin chain genes and the alpha beta gamma and delta T-cell receptor genes. Thus not all tetraploidizations are lethal. Why? I dont' know. Neither does anyone else. 2) As mentioned before, there is little to no correlation between either genome size or number of chromosomes with ANYTHING else. Fish have as wide a distribution of genome size as any other vertebrate class. This is probably the strongest evidence that most junk DNA is indeed that. Why? I don't know. Neither does anyone else. Good References Fundamentals of Molecular Evolution Li and Graur an excellent primer on the subject for both the novice and expert Molecular Evolution and the Immunoglobulin Superfamily Hood and Hunkapiller in "Evolution of Life" (Osawa and Honjo eds) 1991 Implications of the Diversity of the Immunoglobulin Gene Superfamily Hunkapiller, Goverman, Koop, and Hood CSH Symposia on Quantitative Biology 54:15-29 1989 Genome Size Evolution in Vertebrates: Trends and Constants Olmo, Capriglione, and Odierna Comp Biochem Phys 92B:447-453 1989 nice graphs Variations in Genome Mass Wachtel and Tiersch Comp Biochem Phs 104B(2):207-213 1993 --------------------------------------------------- Jared Roach 225 Fluke Hall, Box 352145 Department of Molecular Biotechnology University of Washington Seattle, WA 98195 phone 685-7338 FAX 685-7301 roach@u.washington.edu From owner-chromosomes@net.bio.net Fri Jul 14 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 15 Jul 1995 19:47:01 GMT Organization: McGill University Computing Centre Lines: 21 Message-ID: <3u95vl$50d@sifon.cc.mcgill.ca> References: <3u73m5$5pc@nntp3.u.washington.edu> NNTP-Posting-Host: s-10.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Hello Jared, Although we use the term "junk DNA" quite frequently, it is becoming apparent that these noncoding, intervening sequences may actually have something to do with the organisation of chromosomes, topologically, within the nucleus. For instance, it may be that certain DNA-binding molecules interact with some sort of motif (sequence or 3D) within these regions, and mediate their attachment to the nuclear matrix (akin to matrix arttachment regions). It could be that these areas are important during crossover events as well. I can't remember the particular article, but there is preliminary evidence in support of these notions. On a related vein, has anyone read Gunter Blobel's gene gating hypothesis? It was published in 1985 in PNAS (I can't remember the volume offhand..), and he proposes that the 3D structure of the genome changes throughout development and that this may have some effect on gene transcription. Could it be that this "junk DNA" is fundamentally important for the topological dynamism (assuming his hypothesis is correct) of the genome of an organism during development (or disease, for that matter!!) ? Nick. From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!agate!news.duke.edu!godot.cc.duq.edu!hudson.lm.com!newsfeed.pitt.edu!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 16 Jul 1995 01:58:53 GMT Organization: McGill University (Exp. Medicine) Lines: 53 Message-ID: <3u9rot$i1k@sifon.cc.mcgill.ca> References: <3u73m5$5pc@nntp3.u.washington.edu> NNTP-Posting-Host: o-04.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) You wrote - roach@u.washington.edu (Jared Roach) writes: - I thought I might add a couple comments. - - 1) The evidence is overwhelming that there have been several independent - tetraploidizations during vertebrate evolution. Most of the interesting - papers (with the hardest data) come from the Salmonid geneticists; look in - Medline for these. Also, up my own alley, the vertebrate immune system - clearly arose in its present form following a tetraploidization. It is - the simplest and perhaps only reasonable explanation for the parallel - genomic structure of the heavy and light immunoglobulin chain genes and - the alpha beta gamma and delta T-cell receptor genes. - Thus not all tetraploidizations are lethal. Why? I dont' know. - Neither does anyone else. - I thought that too at first... but this is just a theory and the fact that we can't forseeably resolve tetraploidizations easily with the recipient of the new "scrambled" genome being able to mysteriously procreate. The other extreme is to say that no genome tetraploidizations occured and that all gene families arose by gene conversion (say for the four HOX clusters), perhaps primed by all those repeats in the "junk" DNA as you termed it( LIne-1 and Alu's etc); and then local amplification into clusters of similar genes at one chromosomal site via "slip pairing" during replication or by unequal crossover (to produce individual HOX genes in each cluster). An interesting compromise.... is proposed by Richard Gordon and C. Cristofe Martin in " Differentiatin Trees, a Junk DNA molecular clock, and the evolution of neoteny in salamanders" J. or Evolutionary Biology (1995) I don't have the full ref. with me... sorry. later, Graham _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Genetic Engineering... Date: 16 Jul 1995 12:07:19 GMT Organization: McGill University (Exp. Medicine) Lines: 38 Message-ID: <3uavdn$b7g@sifon.cc.mcgill.ca> References: <3uaq6o$26v@core.apana.org.au> NNTP-Posting-Host: a-10.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) - bennyboy@suburbia.net (Benjamin Hiu Tung Lai) writes: - Hi, - - I am cureently doing an investigation report on Genetic Engineering. - However, I need to report on a more specific topic/application. I am just - curious what are some of the examples (applications) relating to the use of genetic - engineering, other than obtaining pharmaceutical proteins from transgenic - animals. - - - Regards, - Beni - >>>> Tomatoes!!! -they transfered a gene (enzyme) from a pig so that they would remain firm longer sorry can't remeber the details beyond that. Plants in general are light ahead in genetic engineering than animal studies..... G. _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!aggedor.rmit.EDU.AU!goanna.cs.rmit.edu.au!core.apana.org.au!suburbia.net!bennyboy From: bennyboy@suburbia.net (Benjamin Hiu Tung Lai) Newsgroups: bionet.genome.chromosomes Subject: Genetic Engineering... Date: 16 Jul 1995 10:38:16 GMT Organization: Australian Public Access Network Association Lines: 11 Message-ID: <3uaq6o$26v@core.apana.org.au> NNTP-Posting-Host: suburbia.apana.org.au X-Newsreader: TIN [version 1.2 PL2] Hi, I am cureently doing an investigation report on Genetic Engineering. However, I need to report on a more specific topic/application. I am just curious what are some of the examples (applications) relating to the use of genetic engineering, other than obtaining pharmaceutical proteins from transgenic animals. Regards, Beni From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!AIX1.UOTTAWA.CA!s060965 From: s060965@AIX1.UOTTAWA.CA Newsgroups: bionet.genome.chromosomes Subject: Re: 23 pairs of chromosomes/junk DNA? Date: 15 Jul 1995 20:18:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3u9rot$i1k@sifon.cc.mcgill.ca> NNTP-Posting-Host: net.bio.net Hi Graham, The full reference is: C.C. Martin & R. Gordon (1995). Differentiation trees, a junk DNA molecular clock, and the evolution of neoteny in salamanders. J. evol. Biol. Vol 8: pages 339-354. > > An interesting compromise.... is proposed by Richard Gordon and C. Cristofe Martin > in " Differentiatin Trees, a Junk DNA molecular clock, and the evolution of neoteny in salamanders" > J. or Evolutionary Biology (1995) > > I don't have the full ref. with me... sorry. > > later, > > Graham > > > _______________________________________________________________________ > Graham Dellaire Snail Mail: > Red Cross, Research > McGill University Montreal Blood Services > Faculty of Medicine 3131 Sherbrooke St. East > Div. of Experimental Medicine Montreal, QC, Canada > E-mail: popa0206@po-box.mcgill.ca H1W 1B2 > B2XE@musicb.mcgill.ca > WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html > Fax: (514) 525 0881 > Voice: (514) 527 1501 ext 175 > _______________________________________________________________________ > > > > From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.moneng.mei.com!howland.reston.ans.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!fdn.fr!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Re: Why are there 23 pairs of chromosomes? Date: 16 Jul 1995 02:18:23 GMT Organization: McGill University (Exp. Medicine) Lines: 63 Message-ID: <3u9stf$i1k@sifon.cc.mcgill.ca> References: <3u95vl$50d@sifon.cc.mcgill.ca> NNTP-Posting-Host: o-04.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) > > On a related vein, has anyone read Gunter Blobel's gene gating hypothesis? It was published in 1985 > in PNAS (I can't remember the volume offhand..), and he proposes that the 3D structure of the genome > changes throughout development and that this may have some effect on gene transcription. Could it be > that this "junk DNA" is fundamentally important for the topological dynamism (assuming his hypothesis is > correct) of the genome of an organism during development (or disease, for that matter!!) ? > Hello Nick, I am a firm believer that all the so called "junk" in the genome has function... whether it is in maintaining regions of homology for "gene conversion and recombination" during chromosome pairing in meiosis or perhaps more fundementally in control of transcription and replication of different regions of the genome... some recent data has shown that this "junk" may be transcribed into RNA that is functional... ex. XIST RNA which appears to be involved in X-chromosome inactivation or Lin-4 which bind the 3' UTR of the RNA of another protein LIN-6 and prevents its translation in C. elegans. I think genes have been focused on for too long while the context of the gene has been ignored... probably because alot of the pioneering work has been done bacteria and yeast who have a paucity of "junk" dna and are gene "rich" genomes. Someone asked me once why I wasn't in Yeast or bacteria as I wanted to study recombination..? I am in mammalian genetics and I reply only that someone has to do it! .... The system is infinately more complicated and down right stubborn but it is after all a little closer to home. But I am not dumping on Yeast or Bacterial genetics.... thank God for these organisms and the body of research on them.... without it I wouldn't know where to start and articles in my field would be far and few between. Graham > Nick. > > >>>> _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Ref. FOR gating Hypothesis Gunter Blobel Date: 16 Jul 1995 02:05:26 GMT Organization: McGill University (Exp. Medicine) Lines: 29 Message-ID: <3u9s56$i1k@sifon.cc.mcgill.ca> NNTP-Posting-Host: o-04.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Hey, If anyone is interested I pulled up the reference for Gunter Blobels article on gene gating it is Title | Gene gating: a hypothesis. | Source | Proceedings of the National Academy of Sciences of the United States of | America. 82(24):8527-9, 1985 Dec. Cheers Graham _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Re: Genetic Engineering... Date: 16 Jul 1995 12:29:06 GMT Organization: McGill University Computing Centre Lines: 15 Message-ID: <3ub0mi$c4q@sifon.cc.mcgill.ca> References: <3uaq6o$26v@core.apana.org.au> NNTP-Posting-Host: a-07.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Hello Benjamin, As Graham said, genetic engineering is being used in agriculture to improve the yield and robustness of tomatoes, as well as their resistance against certain parasites. Recently, the US government has approved the release of modified microorganisms that can convert pollutants into harmless substances. These microorganisms glow when active, so it is not hard to track them down as they do their work. Detergents: A modified subtilisin gene is being used in certain laundry detergents. And, of course, gene therapy, which promises (once the targeting systems have been optimised) to replace nonfunctioning genes, causing disease, with the normal version. That's all I can think of offhand......... Nick From owner-chromosomes@net.bio.net Sat Jul 15 23:00:00 1995 Path: biosci!aol.com!Banstead From: Banstead@aol.com Newsgroups: bionet.genome.chromosomes Subject: unsubscribe help Date: 16 Jul 1995 11:08:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <950716140635_116011074@aol.com> NNTP-Posting-Host: net.bio.net First of all, sorry to trouble you all! How do you unsubscribe from this list? I have tried everything but to no avail! From owner-chromosomes@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!usc!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Nick Giannoukakis Newsgroups: bionet.genome.chromosomes Subject: Gene Gating Date: 16 Jul 1995 23:53:44 GMT Organization: McGill University Computing Centre Lines: 13 Message-ID: <3uc8q8$i0j@sifon.cc.mcgill.ca> NNTP-Posting-Host: u-05.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Hi there all, I was just wondering if anyone has read Blobel's gene gating hypothesis paper (see Graham Dellaire's post for the actual reference). What do you all think about it? What is interesting is that in 1985, when it was published, Blobel was laughed at. In light of recent observations which support some aspects of the hypothesis, he may actually get the last laugh. His most esoteric proposals (and more exciting, I think) remain untested. I must profess that I am biased in its favor. Nick. From owner-chromosomes@net.bio.net Sun Jul 16 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!news.u.washington.edu!root From: Doug Chapman Newsgroups: bionet.genome.chromosomes Subject: New ISCN avaliable? Date: 17 Jul 1995 21:28:24 GMT Organization: University of Washington Medical Center Lines: 6 Message-ID: <3ueklo$h98@nntp3.u.washington.edu> NNTP-Posting-Host: pete.pathology.washington.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:bionet.genome.chromosomes Does anyone know if a new publication of "AN INTERNATIONAL SYSTEM FOR CYTOGENETIC NOMENCLATURE" is due soon? I've only heard a rumor to that effect but haven't been able to track down any specifics. The publisher is Karger. Any help would be appreciated. From owner-chromosomes@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: m.rocchi@area.ba.cnr.it (ROCCHI) Newsgroups: bionet.genome.chromosomes Subject: YAC BAC probes Date: 18 Jul 1995 07:38:50 +0100 Lines: 13 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3ufktq$jk3@mserv1.dl.ac.uk> X-Sender: e013mr02@193.205.35.100 Original-To: biochrom@dl.ac.uk I am interested in listing any Internet site from which you can get data on physical mapping (FISH in particular) of YAC, BAC, or cosmid probes that could be useful in molecular cytogenetics. Dr. Mariano Rocchi Ist. Genetica Via Amendola 165/A 70126 Bari - Italy tl +39-80-544.3371 fax +39-80-544.3386 m.rocchi@area.ba.cnr.it (new!) From owner-chromosomes@net.bio.net Mon Jul 17 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!sunews!suma3!abralbti From: Emidio Albertini Newsgroups: bionet.genome.chromosomes Subject: PRINS Date: Tue, 18 Jul 1995 19:58:02 +0100 Organization: University of Reading, U.K. Lines: 2 Message-ID: NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I'd like to know more information about PRINS. Thanks a lot. MIMMO From owner-chromosomes@net.bio.net Tue Jul 18 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: NEW!!Experimental Medicine WEB PAGE!! Date: 19 Jul 1995 03:49:16 GMT Organization: McGill University (Exp. Medicine) Lines: 56 Message-ID: <3uhvbs$ffa@sifon.cc.mcgill.ca> NNTP-Posting-Host: a-04.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) McGill Dept. of Medicine Div. of Experimental Medicine Experimental Medicine Home Page http://www.medcor.mcgill.ca/EXPMED/expmed.html Gives a full account of Doing a Ph.D. in the dept -how to apply -finances -requirements -courses -thesis committee selection -defense of thesis -activities Medical Computing resources at McGill, Bio Medical Server and information on PGSS (Post Graduate Students Society) As well as plenty of links and FAQ's on many useful "net" resources available for Genetics/Molecular biology ex. GENEMARK NETSIFTERS NCBI NIH INSTITUTE PASTEUR OMIM Golgi at HARVARD ICGEB at Trieste SUNY Molecular Genetics Server GDB SWISS PIR/EMBL/GENBANK All comments are welcome Graham Dellaire (WEBMASTER) _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!rutgers!oitnews.harvard.edu!bloch.nmr.mgh.harvard.edu!atgw-wel-800d-1-c3.mgh.harvard.edu!user From: dolan@molbio.mgh.harvard.edu (Maureen Dolan) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.arabidopsis,,bionet.genome.chromosomes,bionet.celegans,bionet.molbio.yeast Subject: 384 pin tool for biomek 1000 Date: Wed, 19 Jul 1995 21:56:43 -0500 Organization: Massachusetts General Hospital Lines: 10 Message-ID: NNTP-Posting-Host: atgw-wel-800d-1-c3.mgh.harvard.edu Xref: biosci bionet.molbio.methds-reagnts:31117 bionet.genome.arabidopsis:3556 bionet.genome.chromosomes:725 bionet.celegans:476 bionet.molbio.yeast:3347 Could anyone provide me with info regarding the commercial availability of a 384 pin tool for the Biomek1000? Your help is much appreciated. -- Maureen Dolan, Ph.D. Dept. of Molecular Biology Massachusetts General Hospital Boston, MA 02114 dolan @molbio.mgh.harvard.edu From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!Germany.EU.net!nntp.gmd.de!news.rwth-aachen.de!news.rhrz.uni-bonn.de!news.uni-stuttgart.de!news.belwue.de!fu-berlin.de!rldb3.rz-berlin.mpg.DE!not-for-mail From: Simon Mercer Newsgroups: bionet.genome.chromosomes Subject: Re: YAC BAC probes Date: 20 Jul 1995 12:27:12 GMT Lines: 28 Message-ID: <3uli30$lgj@fu-berlin.de> References: <3ufktq$jk3@mserv1.dl.ac.uk> NNTP-Posting-Host: rldb3.rz-berlin.mpg.de (141.14.130.49) Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Access: 16 25 816 X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.4 sun4m) X-URL: news:3ufktq$jk3@mserv1.dl.ac.uk m.rocchi@area.ba.cnr.it (ROCCHI) wrote: >I am interested in listing any Internet site from which you can get data on >physical mapping (FISH in particular) of YAC, BAC, or cosmid probes that >could be useful in molecular cytogenetics. > >Dr. Mariano Rocchi >Ist. Genetica >Via Amendola 165/A >70126 Bari - Italy >tl +39-80-544.3371 >fax +39-80-544.3386 >m.rocchi@area.ba.cnr.it (new!) > Try the Reference Library Database, which distributes genomic library filters and clones (YACs PACs cosmids, etc) for a number of species. http://gea.lif.icnet.uk/ on the Worldwide Web Simon -- *--------------------------------------------------------------------* Dr. Simon Mercer | The Reference Library Database| Email: Data@rldb.rz-berlin.mpg.de http://gea.lif.icnet.uk/ | Fax : +49 30 8413 1395 From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!uunet!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: gd Newsgroups: bionet.genome.chromosomes Subject: Anyone found any good FTP/WEB site for Shareware/freeware Date: 20 Jul 1995 12:33:25 GMT Organization: McGill University (Exp. Medicine) Lines: 31 Message-ID: <3uliel$7m0@sifon.cc.mcgill.ca> NNTP-Posting-Host: a-07.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) Hello All, I am trying to compile a list of molecular biology/genetics shareware(freeware) FTP and web sites If you have any favorites please send me there addresses. thank you in advance, Graham p.s. I invite you to check out my Dept. Web Page http://www.medcor.mcgill.ca/EXPMED/expmed.html all comments are welcome _______________________________________________________________________ Graham Dellaire Snail Mail: Red Cross, Research McGill University Montreal Blood Services Faculty of Medicine 3131 Sherbrooke St. East Div. of Experimental Medicine Montreal, QC, Canada E-mail: popa0206@po-box.mcgill.ca H1W 1B2 B2XE@musicb.mcgill.ca WWW Page: http://www.medcor.mcgill.ca/EXPMED/expmed.html Fax: (514) 525 0881 Voice: (514) 527 1501 ext 175 _______________________________________________________________________ From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!Norway.EU.net!EU.net!news2.EUnet.fr!ceph.cephb.fr!glibert From: glibert@ceph.cephb.fr (Glibert Fabrice) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.arabidopsis,,bionet.genome.chromosomes,bionet.celegans,bionet.molbio.yeast Subject: Re: 384 pin tool for biomek 1000 Date: 20 Jul 1995 10:43:46 +0200 Organization: Fondation J. Dausset - CEPH Lines: 17 Distribution: world Message-ID: <3ul502$3f2@ceph.cephb.fr> References: NNTP-Posting-Host: ceph.cephb.fr Xref: biosci bionet.molbio.methds-reagnts:31133 bionet.genome.arabidopsis:3562 bionet.genome.chromosomes:727 bionet.celegans:478 bionet.molbio.yeast:3350 In article , dolan@molbio.mgh.harvard.edu (Maureen Dolan) writes: |> |> Could anyone provide me with info regarding the commercial availability of |> a 384 pin tool for the Biomek1000? Your help is much appreciated. |> |> -- |> Maureen Dolan, Ph.D. |> Dept. of Molecular Biology |> Massachusetts General Hospital |> Boston, MA 02114 |> dolan @molbio.mgh.harvard.edu and something about the same tool for the biomek 2000 ??? fabrice From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!daresbury!is.bbsrc.ac.uk!pc0519.ri.bbsrc.ac.uk!user From: Andy.Law@bbsrc.ac.uk (Andy Law (Big Nose)) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.arabidopsis,,bionet.genome.chromosomes,bionet.celegans,bionet.molbio.yeast Subject: Re: 384 pin tool for biomek 1000 Date: Thu, 20 Jul 1995 09:35:44 +0000 Organization: Roslin Institute Lines: 13 Message-ID: References: NNTP-Posting-Host: pc0519.ri.bbsrc.ac.uk X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ Xref: biosci bionet.molbio.methds-reagnts:31131 bionet.genome.arabidopsis:3561 bionet.genome.chromosomes:726 bionet.celegans:477 bionet.molbio.yeast:3349 In article , dolan@molbio.mgh.harvard.edu (Maureen Dolan) wrote: > Could anyone provide me with info regarding the commercial availability of > a 384 pin tool for the Biomek1000? Your help is much appreciated. > Have you asked Beckman? -- Andy Law ( Andy.Law @ bbsrc.ac.uk ) ( Big Nose in Edinburgh ) From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!geraldo.cc.utexas.edu!usenet From: Kreblon@ccwf.cc.utexas.edu (Robert Nagy) Newsgroups: bionet.genome.chromosomes Subject: Seed formation in an intergeneric rice hybrid Date: 20 Jul 1995 20:42:16 GMT Organization: The University of Texas at Austin Lines: 19 Message-ID: <3umf38$2su@geraldo.cc.utexas.edu> NNTP-Posting-Host: kreblon.botany.utexas.edu X-Newsreader: WinVN 0.90.4 Dear colleagues: In the last Annual Conference of the Bangladesh Association for Plant Tissue Culture (BAPTC) held in Dhaka in April last, a paper was presented reporting for the first time a hybrid at the tetraploid level in the combination, colchicine-induced tetraploid rice, c-4x Oryza sativa (2n=4x=48) x a natural tetraploid, Porteresia coarctata (2n+48), which grows wild in the coastal areas of the Indian subcontinent. The author said that in spite of a large number of backcrosses to either of the parents and self-pollination, not a single seed was formed. Surprisingly, Pollen stainability was better and there was less variation in the size of pollen compared to those of the c-4x O. sativa. Could anyone please suggest of any modern technique which will promote the setting of seeds because if the researcher obtains one viable seed, it will open an unlimited possibility of creating rice varieties suitable for growing in the saline coastal soil where no food crops grows at present. I shall very much appreciate receiving a reply which I would like to communicate to the researcher who does not have facility to an internet connection. Thanks again. Sincerely, Ahmad Islam From owner-chromosomes@net.bio.net Wed Jul 19 23:00:00 1995 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!usenet From: Alex Copeland Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.arabidopsis,,bionet.genome.chromosomes,bionet.celegans,bionet.molbio.yeast Subject: Re: 384 pin tool for biomek 1000 Date: 20 Jul 1995 15:03:47 GMT Organization: Lawrence Livermore National Laboratory Lines: 22 Message-ID: <3ulr8j$1k3@lll-winken.llnl.gov> References: <3ul502$3f2@ceph.cephb.fr> NNTP-Posting-Host: cosmid.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.3_U1 sun4m) X-URL: news:3ul502$3f2@ceph.cephb.fr Xref: biosci bionet.molbio.methds-reagnts:31154 bionet.genome.arabidopsis:3563 bionet.genome.chromosomes:730 bionet.celegans:481 bionet.molbio.yeast:3353 glibert@ceph.cephb.fr (Glibert Fabrice) wrote: > >In article , dolan@molbio.mgh.harvard.edu (Maureen Dolan) writes: >|> >|> Could anyone provide me with info regarding the commercial availability of >|> a 384 pin tool for the Biomek1000? Your help is much appreciated. >|> > > >and something about the same tool for the biomek 2000 ??? > >fabrice The Helix division of General Atomics, which may now be defunct, was manufacturing a 384-pin tool compatible with the Biomek. The Biomek 1000 and 2000, I believe, use the same mounting hardware. alex From owner-chromosomes@net.bio.net Fri Jul 21 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!trane.uninett.no!Norway.EU.net!EU.net!news.sprintlink.net!europa.chnt.gtegsc.com!usenet.eel.ufl.edu!usenet.cis.ufl.edu!caen!kuhub.cc.ukans.edu!crs!ksilverstein Newsgroups: bionet.genome.chromosomes Subject: Is there a Karyotype Parser available? Message-ID: <1995Jul21.074607.1@crs> From: ksilverstein@crs.stjude.org Date: 21 Jul 95 07:46:07 CST Organization: St Jude Children's Research Hospital Nntp-Posting-Host: crs.stjude.org Lines: 8 Has anyone developed grammar or parser software for parsing karyotype reports? TIA -- Karen Silverstein karen.silverstein@stjude.org "Speaking only for myself" St Jude Children's Research Hospital, (901)531-2375 332 N. Lauderdale Avenue, Memphis, TN 38101 From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!tank.news.pipex.net!pipex!news.uoregon.edu!news.bc.net!unixg.ubc.ca!vanbc.wimsey.com!fonorola!news!news From: pbthrgch@magi.com Subject: (no subject) Sender: news@magi.com Message-ID: Date: Sun, 23 Jul 1995 20:15:34 GMT Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Nntp-Posting-Host: magi01p26.magi.com Mime-Version: 1.0 X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Organization: Magi Data Consulting Lines: 9 HI all! I'm interested to know what are the resources available on the "cri du chat" syndrome ( also called 5p-) on the net. Thank you. PBouthillier From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!tank.news.pipex.net!pipex!news.uoregon.edu!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.starway.net.au!news.studioweb.com!usenet From: Brendon Chua Newsgroups: bionet.genome.chromosomes Subject: HELP!!:Gene Therapy for Huntington's Disease Date: 25 Jul 1995 10:44:32 GMT Organization: Connexus Internet Lines: 15 Message-ID: <3v2hug$1hq@studioweb.com> NNTP-Posting-Host: dialin-4.connexus.apana.org.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: All I'm currently doing a thesis on the recent dicovery of the gene segment on chromosome 4 that causes Huntington's disease. I am in urgent need of any information on the gene therapies and genetic treatments availible for this disease if any. I would be extremly grateful to hear from professors or doctors that can help me with my research. Thank You. Brendan From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!tank.news.pipex.net!pipex!news.uoregon.edu!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.starway.net.au!news.studioweb.com!usenet From: Brendon Chua Newsgroups: bionet.genome.chromosomes Subject: HELP!!:Gene Therapy for Huntington's Disease Date: 25 Jul 1995 10:44:03 GMT Organization: Connexus Internet Lines: 15 Message-ID: <3v2htj$1hq@studioweb.com> NNTP-Posting-Host: dialin-4.connexus.apana.org.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: All to anyone out there that can help me, I'm currently doing a thesis on the recent dicovery of the gene segment on chromosome 4 that causes Huntington's disease. I am in urgent need of any information on the gene therapies and genetic treatments availible for this disease if any. I would be extremly grateful to hear from professors or doctors that can help me with my research. Thank You. Brendan From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!gatech!news.uoregon.edu!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.starway.net.au!news.studioweb.com!usenet From: Brendon Chua Newsgroups: bionet.genome.chromosomes Subject: HELP!!:Gene Therapy for Huntington's Disease Date: 25 Jul 1995 10:43:14 GMT Organization: Connexus Internet Lines: 12 Message-ID: <3v2hs2$1hq@studioweb.com> NNTP-Posting-Host: dialin-4.connexus.apana.org.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: All to anyone out there that can help me, I'm currently doing a thesis on the recent dicovery of the gene segment on chromosome 4 that causes Huntington's disease. I am in urgent need of any information on the gene therapies and genetic treatments availible for this disease if any. I would be extremly grateful to hear from professors or doctors that can help me with my research. Thank You. Brendan From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!gatech!news.uoregon.edu!vixen.cso.uiuc.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.starway.net.au!news.studioweb.com!usenet From: Brendon Chua Newsgroups: bionet.genome.chromosomes Subject: HELP!!:Gene Therapy for Huntington's Disease Date: 25 Jul 1995 10:42:36 GMT Organization: Connexus Internet Lines: 12 Message-ID: <3v2hqs$1hq@studioweb.com> NNTP-Posting-Host: dialin-4.connexus.apana.org.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: All To anyone out there that can help me, I'm currently doing a thesis on the recent dicovery of the gene segment on chromosome 4 that causes Huntington's disease. I am in urgent need of any information on the gene therapies and genetic treatments availible for this disease if any. I would be extremly grateful to hear from professors or doctors that can help me with my research. Thank You. Brendan From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!rutgers!gatech!news.sprintlink.net!news.gate.net!tpafl-32.gate.net!biometra From: biometra@gate.net (Tony Sanchez) Newsgroups: bionet.genome.chromosomes,bionet.molbio.gene-linkgage,bionet.molbio.genome-program Subject: Info on Scotlabs Date: Tue, 25 Jul 1995 10:31:43 Organization: Biometra Inc. Lines: 8 Message-ID: NNTP-Posting-Host: tpafl-32.gate.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Xref: biosci bionet.genome.chromosomes:741 bionet.molbio.genome-program:1465 I am interested in getting opinions on a company called Scotlabs. In particular, I am interested in hearing about experiences with their Nucleon DNA extraction kit. Thanks in advance, Tony Sanchez From owner-chromosomes@net.bio.net Mon Jul 24 23:00:00 1995 Path: biosci!rutgers!uwm.edu!msunews!harbinger.cc.monash.edu.au!yarrina.connect.com.au!news.starway.net.au!news.studioweb.com!usenet From: Brendon Chua Newsgroups: bionet.genome.chromosomes Subject: HELP!!:Gene Therapy for Huntington's Disease Date: 25 Jul 1995 10:45:04 GMT Organization: Connexus Internet Lines: 16 Message-ID: <3v2hvg$1hq@studioweb.com> NNTP-Posting-Host: dialin-4.connexus.apana.org.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To anyone that can help me: I'm currently doing a thesis on the recent dicovery of the gene segment on chromosome 4 that causes Huntington's disease. I am in urgent need of any information on the gene therapies and genetic treatments availible for this disease if any. I would be extremly grateful to hear from professors or doctors that can help me with my research. Thank You. Brendan From owner-chromosomes@net.bio.net Tue Jul 25 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.uoregon.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!acer.gen.tcd.ie!dbarton From: dbarton@acer.gen.tcd.ie (Dr David E Barton) Subject: Re: HELP!!:Gene Therapy for Huntington's Disease Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Irish National Centre for Bioinformatics References: <3v2hs2$1hq@studioweb.com> Date: Wed, 26 Jul 1995 13:24:11 GMT Lines: 26 In article <3v2hs2$1hq@studioweb.com>, Brendon Chua wrote: >I'm currently doing a thesis on the recent dicovery of the gene segment >on chromosome 4 that causes Huntington's disease. I am in urgent need of >any information on the gene therapies and genetic treatments availible >for this disease if any. As far as I am aware, there is no treatment available, and developing gene therapy is going to be a formiddable challenge, because the tissues involved as so inaccessable, and because the disease is dominant. That is to say that every cell in an affected individual already has a normal copy of the HD gene in it, so just getting a normal gene in is not going to work, like it would for an enzyme deficiency. Therapy is likely to be directed at knocking out the mutant gene, but how do you knock out a gene with 40 repeats and leave the normal one, which might have 30 repeats, intact? All suggestions welcomed!! | David Barton | National Centre for Medical Genetics | Our Lady's Hospital for Sick Children | Crumlin, Dublin 12, Ireland. | Tel +353 1 455 0515 Fax 455 8873 From owner-chromosomes@net.bio.net Wed Jul 26 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!swrinde!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: littletwo@aol.com (LittleTwo) Newsgroups: bionet.genome.chromosomes Subject: horse genome mapping Date: 26 Jul 1995 21:10:45 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <3v6p2l$rr6@newsbf02.news.aol.com> Reply-To: littletwo@aol.com (LittleTwo) NNTP-Posting-Host: newsbf02.mail.aol.com >is there a horse genome mapping? >how can you get access? >from www >is the male or female the heterogametic sex? From owner-chromosomes@net.bio.net Wed Jul 26 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!gatech!paladin.american.edu!news.ecn.uoknor.edu!news.uoknor.edu!ns1.nodak.edu!badlands!dahleen From: dahleen@badlands.NoDak.edu (Lynn S Dahleen) Subject: Re: Seed formation in an intergeneric rice hybrid Sender: usenet@ns1.nodak.edu (Usenet login) Message-ID: Date: Thu, 27 Jul 1995 17:53:46 GMT References: <3umf38$2su@geraldo.cc.utexas.edu> Nntp-Posting-Host: badlands.nodak.edu Organization: North Dakota Higher Education Computing Network X-Newsreader: TIN [version 1.2 PL2] Lines: 27 We've had a similar problem getting backcross seed from wide hybrid plants. We now spray our crosses with a dilute giberellin solution to get seed set. We usually spray 24 hours after pollination. If you need more information, e-mail me. Lynn Dahleen dahleen@badlands.nodak.edu Robert Nagy (Kreblon@ccwf.cc.utexas.edu) wrote: : Dear colleagues: : In the last Annual Conference of the Bangladesh Association for Plant Tissue Culture (BAPTC) held in Dhaka in : April last, a paper was presented reporting for the first time a hybrid at the tetraploid level in the combination, : colchicine-induced tetraploid rice, c-4x Oryza sativa (2n=4x=48) x a natural tetraploid, Porteresia coarctata : (2n+48), which grows wild in the coastal areas of the Indian subcontinent. The author said that in spite of a : large number of backcrosses to either of the parents and self-pollination, not a single seed was formed. Surprisingly, : Pollen stainability was better and there was less variation in the size of pollen compared to those of the : c-4x O. sativa. : : Could anyone please suggest of any modern technique which will promote the setting of seeds because : if the researcher obtains one viable seed, it will open an unlimited possibility of creating rice varieties suitable for : growing in the saline coastal soil where no food crops grows at present. : I shall very much appreciate receiving a reply which I would like to communicate to the researcher who : does not have facility to an internet connection. Thanks again. : Sincerely, : Ahmad Islam From owner-chromosomes@net.bio.net Wed Jul 26 23:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!news.tamu.edu!vms1.tamu.edu!pkr1674 From: pkr1674@vms1.tamu.edu (PENNY RIGGS) Newsgroups: bionet.genome.chromosomes Subject: Re: horse genome mapping Date: 27 Jul 1995 09:19 CDT Organization: Texas A&M University OpenVMScluster Lines: 21 Distribution: world Message-ID: <27JUL199509190357@vms1.tamu.edu> References: <3v6p2l$rr6@newsbf02.news.aol.com> NNTP-Posting-Host: vms1.tamu.edu News-Software: OpenVMS VNEWS 1.41 In article <3v6p2l$rr6@newsbf02.news.aol.com>, littletwo@aol.com (LittleTwo) writes... >>is there a horse genome mapping? Yes, several groups are involved in horse genome projects. >>how can you get access? >>from www My understanding is that databases are being set up. A web page is maintained by Lee Millon in Dr. Ann Bowling's group. You can contact him by email at lvmillon@ucdavis.edu A workshop on the horse genome is scheduled for October. For info on that you can contact Teri Lear at equigene@ukcc.uky.edu or Dr. Ernie Bailey at vsc003@ukcc.uky.edu >>is the male or female the heterogametic sex? The horse has 64 chromosomes -- a mixture of meta- and submetacentric and acrocentric chromosomes. The male is heterogametic, XY. -- Penny K. Riggs Department of Veterinary Pathobiology, pk-riggs@tamu.edu Texas A&M Univ., College Station, TX 77843 From owner-chromosomes@net.bio.net Thu Jul 27 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!convex!news.duke.edu!usenet From: marcy@duke.edu (Marcy Speer) Newsgroups: bionet.genome.chromosomes Subject: mouse genetics Date: 28 Jul 1995 17:56:24 GMT Organization: Duke University Medical Center Lines: 13 Distribution: world Message-ID: <3vb8c8$60k@news.duke.edu> References: <27JUL199509190357@vms1.tamu.edu> Reply-To: marcy@duke.edu NNTP-Posting-Host: genemap.mc.duke.edu Hi - has anyone seen a description of haplotyping in the mouse literature (in other words, assigning parental origin of alleles to offspring with the intent of scoring recombination events)? I am looking for a "how-to" type reference. Thanks - Marcy Speer From owner-chromosomes@net.bio.net Sun Jul 30 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!agate!msunews!harbinger.cc.monash.edu.au!aggedor.rmit.EDU.AU!minyos.xx.rmit.EDU.AU!t9556457 From: t9556457@minyos.xx.rmit.EDU.AU (Chun Ming Chan) Newsgroups: bionet.genome.chromosomes Subject: Engineered Tomatoes Date: 31 Jul 1995 13:05:25 GMT Organization: Royal Melbourne Institute of Technology, Melbourne, Australia. Lines: 14 Message-ID: <3vikel$gre@aggedor.rmit.EDU.AU> NNTP-Posting-Host: minyos.xx.rmit.edu.au NNTP-Posting-User: t9556457 X-Newsreader: TIN [version 1.2 PL2] Subject: Engineered Tomatoes Newsgroups: aus.education.bio-newtech Summary: Keywords: Hi! Anyone knows the antisese gene and othere genes that are being engineered in the Flavr Tomatoes? Does the engineering process only involve the DNA Recombinant technique? thanks Ben From owner-chromosomes@net.bio.net Sun Jul 30 23:00:00 1995 Path: biosci!rutgers!gatech!news.uoregon.edu!news.u.washington.edu!root From: dadler@koko.pathology.washington.edu (David Adler) Newsgroups: bionet.genome.chromosomes Subject: Re: Is there a Karyotype Parser available? Date: 31 Jul 1995 20:56:14 GMT Organization: University of Washington Lines: 26 Message-ID: <3vjg1e$gv1@nntp3.u.washington.edu> References: <1995Jul21.074607.1@crs> NNTP-Posting-Host: koko.pathology.washington.edu In article <1995Jul21.074607.1@crs>, Karen Silverstein writes: > Has anyone developed grammar or parser software for parsing karyotype > reports? I and others here at the University of Washington have worked on an ISCN parser to allow automated drawings of idiographic karyotypes - useful for clinical reports, genetic counseling and training. Without funding lubrication the project moves rather slowly ;( I know that Jan Friedman up in Vancouver, B.C. built a parser used for creating a cytogenetic case registry: Friedman-J-M. Smith-J-P. Lerner-B-N. Helgeson-J-S. Howard-Peebles-P-N. Mize-C-E. Mize-S-G. Singleton-W-L. Smith-M-E. ReCAP: the Registry of Cytogenetic Abnormalities and Phenylketonuria. Am-J-Med-Genet. 1987 Jun. 27(2). P 325-36. AMERICAN JOURNAL OF MEDICAL GENETICS. Hope this is useful, David -- David A. Adler Pathology SM-30 University of Washington Seattle, WA 98195 (206) 543-0716 (phone) (206) 543-3644 (fax) "Science is nothing but trained and organized common sense" T.H.Huxley From owner-chromosomes@net.bio.net Sun Jul 30 23:00:00 1995 Path: biosci!BIOCHEM.DENTAL.UPENN.EDU!wra From: wra@BIOCHEM.DENTAL.UPENN.EDU (Bill Abrams) Newsgroups: bionet.genome.chromosomes Subject: Re: Is there a Karyotype Parser available? Date: 31 Jul 1995 07:23:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <199507311412.KAA04990@biochem.dental.upenn.edu> References: <1995Jul21.074607.1@crs> NNTP-Posting-Host: net.bio.net > > Has anyone developed grammar or parser software for parsing karyotype reports? > > TIA > -- > Karen Silverstein karen.silverstein@stjude.org > "Speaking only for myself" St Jude Children's Research Hospital, > (901)531-2375 332 N. Lauderdale Avenue, > Memphis, TN 38101 > Not sure if the following will help, but... Microsoft Excel allows parsing of text or numbers from AscII text files. This can then be exported to Word of word processor of your choice. -- ------------------------------------------------------------------ William R Abrams, PhD email:wra@biochem.dental.upenn.edu University of Pennsylvania School of Dental Medicine Biopolymer Analysis Laboratory 4001 Spruce Street Telephone: (215) 898-2047 Philadelphia, PA 19104 FAX: (215) 573-2324 ------------------------------------------------------------------ From owner-chromosomes@net.bio.net Mon Jul 31 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!newshost.marcam.com!zip.eecs.umich.edu!panix!usenet From: Gary Welz Newsgroups: bionet.genome.chromosomes Subject: Is a Genome Like a Computer Program Date: 1 Aug 1995 19:25:04 GMT Organization: SETN Lines: 14 Message-ID: <3vlv2g$hnb@news1.panix.com> NNTP-Posting-Host: gwelz.dialup.access.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes The article "Is a Genome Like a Computer Program" that I discussed in this newsgroup several months ago is now published in an electronic journal on the WWW called The X Advisor. The URL of the article is: "http://landru.unx.com/DD/advisor/docs/jul95/welz.genome0.shtml" My thanks to all who offered their comments, many of which are included in the article. I look forward to further discussions on the topics raised. best wishes, Gary Welz WWW home page: http://found.cs.nyu.edu/found.a/CAT/misc/welz/