From owner-chromosomes@net.bio.net Sun Oct 01 23:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!spool.mu.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!swrinde!cs.utexas.edu!news.sprintlink.net!in2.uu.net!cadvision.com!usenet From: Jeff Taylor Newsgroups: bionet.genome.chromosomes Subject: [Re] Chromosome 6 Date: 2 Oct 1995 15:08:30 GMT Organization: CADVision Lines: 49 Message-ID: <44ov9e$106s@cadvision.com> NNTP-Posting-Host: cad138.cadvision.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news://news/bionet.genome.chromosomes nsuyeda@aol.com on 29-Sept-95 writes: >A friend's amniocentesis shows an abnormal wide band on six. The lab's >first response is to ....... -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- This is regarding your question about your friend's amniocentesis. There is a question I would have for you (or her). If they are normal, healthy parents with a normal sonogram why have they had an amnio? I am going to assume the answer to this is because of the mother's age. You say there is a band that is wider than normal. (It would help immensely to know which band). This basically means there is extra DNA present. There are 2 possibilities: 1) The extra DNA does not contain any genes (nor affect any genes normally present in this region). There would most likely be no repercussions to the baby. The only way of knowing this for sure is if one of the parents has the same anomaly. That is why the first thing the lab is doing is to test the parents. 2) The extra DNA contains genes. If this is the case then the baby has one too many copies of the extra gene(s). Because a chromosome analysis is looking for gross chromosomal abnormalities, the fact that they have found something means that there is a lot of extra DNA. This is not good news. To do any kind of research you would have to know the specific band on chromosome 6 that is affected and then go to a medical library (most universities have one). You would then have to check the literature to see if there are any reported cases of people having extra DNA (trisomy) in this band. To date 23 patients with partial trisomy of the long arm of chromosome 6 have been reported. These all involve duplication of more than a single band so are more severe than what you say is a single abnormal wide band. Therefore it is impossible to speculate on your case. The best news would be for one of the parents to have the same phenomenon. Otherwise, I feel there may very well be physical and/or neurological problems. Hilary Taylor (MSc Human & Medical Genetics) From owner-chromosomes@net.bio.net Sun Oct 01 23:00:00 1995 Path: biosci!ODIN.MDA.UTH.TMC.EDU!sa83165 From: sa83165@ODIN.MDA.UTH.TMC.EDU (Jean C. Zenklusen) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome Six Date: 2 Oct 1995 12:15:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 78 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On Sept. 29 Jeff Taylor wrote: >nsuyeda@aol.com on 29-Sept-95 writes: > >>A friend's amniocentesis shows an abnormal wide band on six. The lab's >>first response is to ....... >-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- > >This is regarding your question about your friend's amniocentesis. There is a >question I would have for you (or her). If they are normal, healthy parents >with a normal sonogram why have they had an amnio? I am going to assume the >answer to this is because of the mother's age. > >You say there is a band that is wider than normal. (It would help immensely >to know which band). This basically means there is extra DNA present. There >are 2 possibilities: > >1) The extra DNA does not contain any genes (nor affect any genes normally >present in this region). There would most likely be no repercussions to the >baby. The only way of knowing this for sure is if one of the parents has the >same anomaly. That is why the first thing the lab is doing is to test the >parents. > This is a very unlikely situation since the band amplification has been determined by cytogenetics. Such an amplif ication has to be at least 20Mb long in order to be detected. It is very unlikely that no genes would be found here. >2) The extra DNA contains genes. If this is the case then the baby has one >too many copies of the extra gene(s). Because a chromosome analysis is >looking for gross chromosomal abnormalities, the fact that they have found >something means that there is a lot of extra DNA. This is not good news. > >To do any kind of research you would have to know the specific band on >chromosome 6 that is affected and then go to a medical library (most >universities have one). You would then have to check the literature to see if >there are any reported cases of people having extra DNA (trisomy) in this >band. >>To date 23 patients with partial trisomy of the long arm of chromosome 6 have >been reported. These all involve duplication of more than a single band so >are more severe than what you say is a single abnormal wide band. Therefore it >is impossible to speculate on your case. > The case reported here is not a trisomy but a gross a band amplification. Trisomy only aplies to duplication of an entire chromosome or chromosomal band. This amplifications tend to be highly significative as in the case of several proto-oncogenes that get deregulated by a dose imbalance. Amplifications in chr 6 are very well known for the long arm, but to be certain of which could be the consequences of this trait, one has to know which band is amplified. If nsuyeda@aol.com could provide this information, maybe we could help some more. >The best news would be for one of the parents to have the same phenomenon. >Otherwise, I feel there may very well be physical and/or neurological >problems. > > >Hilary Taylor >(MSc Human & Medical Genetics) ################################################# Jean C. Zenklusen, M.S., Ph. D. Science has "explained" The University of Texas nothing; the more we know, M. D. Anderson Cancer Center the more fantastic the Science Park - Research Division world becomes and the P. O. Box 389 profounder the surrounding Smithville, TX 78957 darkness. TE: (512) 237-9431 Aldous Huxley (1894-1963) sa83165@odin.mda.uth.tmc.edu ################################################# From owner-chromosomes@net.bio.net Sun Oct 01 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!acer.gen.tcd.ie!dbarton From: dbarton@acer.gen.tcd.ie (Dr David E Barton) Subject: Re: Chromosome Six Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Irish National Centre for Bioinformatics References: <44ht18$1vv2@huey.cadvision.com> Date: Mon, 2 Oct 1995 12:50:01 GMT Lines: 22 In article <44ht18$1vv2@huey.cadvision.com>, Jeff Taylor wrote: >nsuyeda@aol.com on 29-Sept-95 writes: > >>A friend's amniocentesis shows an abnormal wide band on six. The lab's >>first response is to ....... I did not see the original question, but I would urge most strongly against acting on any clinical/medical advice that you get on the Internet - There are too many strange people out there to trust the advice you get. Seek out a qualified clinical geneticist for advice. e-mail me the name of your city and I can look one up for you. Best wishes, David. | David Barton | National Centre for Medical Genetics | Our Lady's Hospital for Sick Children | Crumlin, Dublin 12, Ireland. | Tel +353 1 455 0515 Fax 455 8873 From owner-chromosomes@net.bio.net Mon Oct 02 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: nsuyeda@aol.com (N S Uyeda) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome Six Date: 2 Oct 1995 23:09:38 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 9 Sender: root@newsbf02.news.aol.com Message-ID: <44q9hi$n45@newsbf02.news.aol.com> References: Reply-To: nsuyeda@aol.com (N S Uyeda) NNTP-Posting-Host: newsbf02.mail.aol.com To Dr David Barton: Thank you so much for your post on the chromosome news group. I very much agree with your advice. The reason for my question was that the genetics lab (run by qualified geneticist) said they had never seen this band on six before. Since then someone at the Univ of Calif San Francisco has said that he has seen it but rarely. Another source--Southeast U.S. says he sees it "all the time". These are all experts!! The parents of course wanted as much information as possible; but they got some good news and do not have to seek further advice--the father has this same extra DNA. From owner-chromosomes@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!agate!hpg30a.csc.cuhk.hk!CUHK.csc.cuhk.hk!s936117 From: s936117@mailserv.cuhk.hk (Four Students) Newsgroups: bionet.genome.chromosomes Subject: Questionnaire on "Surrogate Motherhood" Date: Thu, 5 Oct 1995 02:14:29 LOCAL Organization: The Chinese University of Hong Kong Lines: 174 Message-ID: NNTP-Posting-Host: @slip057.csc.cuhk.hk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] Questionnaire on "Surrogate Motherhood" Hi, we are four of the students of the Chinese University of Hong Kong and going to graduate next year. Now we are going to make a research about "surrogate motherhood". "Surrogacy" means an infertile married couple (i.e. "commissioning couple") to entrust another woman (i.e. "surrogate mother") to give birth to a baby. After the sperm and the egg of the "commissioning couple" fertilized, the fertilized egg will be put into the surrogate mother's womb. The baby will be given back to the "commissioning couple" after birth. In this questionnaire, we hope to gather the opinions from different people about "surrogacy". All the information is confidential. It is appreciated to everybody who is kind enough to finish the questionnaire. Thanks a lot. (Please put a '*' as your choice.) (Please mail to:s936117@mailserv.cuhk.hk) ------------------------------------------------------------------------- Personal Information: 1. Sex: [ ]Male [ ]Female 2. Are you Chinese? [ ]Yes [ ]No 3. Age: [ ]18-24 [ ]25-32 [ ]33-40 [ ]41-48 [ ]49 or above 4. Marital Status: [ ]Single [ ]Married (including divorced or widowed) 5. Religion: [ ]Christianity [ ]Catholic [ ]Buddhism [ ]Islam [ ]Taoism [ ]Others:_______________ 6. Education level: [ ]Elementary [ ]Secondary [ ]University or other institutes [ ]Others:_______________ ----------------------------------------------------------------------- 7. Have you ever heard about "surrogate mother"? [ ]Yes [ ]No 8. In a consultation paper (1993) in Hong Kong, there are the following suggestions, your opinion is: a. Commercial "surrogacy" (i.e. where an agency or the surrogate mother makes profit out of a surrogate arrangement) should be banned [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment b. "Surrogacy" should be allowed only for infertile married couples where no alternative medical treatment is possible [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment c. A woman who has never been married nor has had a child of her own should not be allowed to act as a "surrogate mother" [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment d. For "surrogacy", the consent of the "surrogate mother" and her husband should be required [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment e. Proper professional counseling on the likely problems for both the "commissioning couple" and the surrogate should be made an integral part of the process before, during and after surrogacy [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment 9. If you or your spouse is infertile, and there is only one choice from the followings, what would you choose? [ ]Seek help from a "surrogate mother's" help [ ]Adopting a child (please go to question 11 directly) 10. Referring to question 9, why do you want to seek help from a "surrogate mother"? (More than one option are acceptable) [ ]Get rid of pain during labour [ ]Maintaining the genetic connection (as the fertilized egg is come from the sperm and egg of the "commissioning married couple") [ ]Avoiding the inconvenience of pregnancy (e.g. losing working ability temporary) [ ]Avoiding the complicated adoption procedure [ ]Worrying about the adopted child's background [ ]Others:________________ 11. If the traditional concept of "Giving Birth should be followed by Nurturing" is valid, do you agree the "surrogate mother" would violate this concept? [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment 12. If somebody says "the surrogate mother would demean woman's status", what is your opinion? [ ]Strongly agree [ ]Agree [ ]Disagree [ ]Strongly disagree [ ]No comment 13. After the baby was born, do you think the "surrogate mother" has the right to visit the baby on a regular basis? [ ]Yes [ ]No 14. Do you think the child has the right to know he/she was born by "surrogacy"? [ ]Yes [ ]No 15. If the child know he/she was born by "surrogate mother", do you think there will be any ill psychological impact? [ ]Yes [ ]No 16. To seek help from a "surrogate mother", there is a whole bunch of fees to pay, e.g. her living, lawyer's fee, hospital fee, etc. Do you think it is unfair to the poor infertile married couple? [ ]Yes [ ]No 17. "Surrogacy" would arouse of some technical problems, which of the followings are likely to occur? (Please rank in order, '1' - most common, '5' - most rare) [ ]The "surrogate mother" demand for a higher reward during her pregnancy [ ]The fetus is unhealthy [ ]The continuity of pregnancy will endanger the "surrogate mother" [ ]The "surrogate mother" refuse to give up the baby after birth [ ]negligence of the "surrogate mother" leads to fetal damage (e.g. smoking, drinking) 18. If you are "commissioning couple", your child come from "surrogacy" is mentally or physically handicapped, what would you do? [ ]Willing to take care of the baby [ ]Not willing to but still take care of the baby [ ]refuse to accept the baby 19. During the pregnancy of the "surrogate mother", if the "commissioning couple" is divorced or one of them is passed away, what is the best arrangement for the fetus? [ ]Continuing the pregnancy, and then the "surrogate mother" nurture the baby [ ]For abortion if possible [ ]Continuing the pregnancy, and then either one spouse of the "commissioning couple" nurture the baby [ ]Continuing the pregnancy, and an appropriate government body would take care of the baby 20. In your opinion, which of the following people should be allowed to use "surrogacy"? (More than one option are acceptable) [ ]Infertile married couples [ ]Couples who are able to carry baby but not yet give birth [ ]Couples who have been given birth [ ]Single people [ ]Homosexual people 21. Which of the following people do you think is suitable to be a "surrogate mother"? (For "Age" and "Education level", more than one option are acceptable) Marital status: [ ]Unmarried [ ]Married Age: [ ]20-26 [ ]27-33 [ ]34-40 Education level: [ ]Elementary [ ]High school [ ]Tertiary 22. Which of the following ways of "surrogacy" is the most acceptable to you? (Please rank in order, '1' - most acceptable, '6' - most unacceptable) [ ]Husband sperm + wife egg + surrogate womb [ ]Husband sperm + surrogate egg and womb [ ]Husband sperm + donor egg + surrogate womb [ ]Donor sperm + wife egg + surrogate womb [ ]Donor sperm + surrogate egg and womb [ ]Donor sperm + donor egg + surrogate womb 23. If the "surrogate mother" is unwilling to give up the baby, in your opinion, which party can own the custody of the baby? [ ]Surrogate mother [ ]Commissioning couple [ ]Government 24. Do you think the children of the "surrogate mother" will have desperate feeling toward the baby in "surrogacy"? [ ]Yes [ ]No 25. Do you think "surrogacy" can help to maintain the marital relationship of the "commissioning couple"? [ ]Yes [ ]No This is the end of the questionnaire. If you have any other opinion in "surrogacy", please state: Thank you for your precious opinion. ------END------ From owner-chromosomes@net.bio.net Wed Oct 04 23:00:00 1995 Path: biosci!biosci!not-for-mail From: lstein@genome.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.genome.chromosomes,bionet.announce Subject: DATA RELEASE 8 OF THE WHITEHEAD/MIT HUMAN GENOME MAPPING PROJECT Date: 4 Oct 1995 19:26:48 -0700 Organization: Whitehead Institute for Biomedical Research Lines: 94 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <44vcid$ksv@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.genome.chromosomes:837 bionet.announce:2517 ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH HUMAN GENOMIC MAPPING PROJECT DATA RELEASE 8 (SEPTEMBER 1995) The eighth release of data from the Human Physical Mapping Project at the Whitehead Institute/MIT Genome Center, covering data generated through the end of September, 1995, is now available. This data release contains YAC screening data for 11,871 sequence tagged sites (STSs) screened on the CEPH mega-YAC library. For each STS, we report addresses for the YACs found to contain the STS. From the data obtained so far, there are 677 contigs assembled using single linkage between STSs. In addition, we also report a radiation hybrid map of the genome containing 5,138 STS markers mapped on the Genebridge Panel, as well as integrations of the genetic, radiation hybrid and YAC contig maps. The data is available electronically in two ways. ANONYMOUS FTP: The entire data release is available as a set of Microsoft Excel files and tab-delimited ascii files on our ftp server. Using an ftp client (such as "Fetch" on the Macintosh), connect to ftp-genome.wi.mit.edu Use "anonymous" as your user name, and give your e-mail address as your password. The data files are present in the directory /distribution/human_STS_releases/sep95. The contents are as follows: 09-95.INTRO.txt Introduction to the data release, in straight text format 09-95.INTRO.html The same in HTML (World Wide Web) format 09-95.STS2YAC.txt STS & YAC screening data as tab-delimited text. 09-95.STS2YAC.sea.hqx The same as a compressed Microsoft Excel File chromosomes/ The same, split into smaller chromosome-specific files 09-95.YAC2STS.txt Inverse map of YAC to STS screening data 09-95.CONTIG2STS.txt YAC contig lists. 09-95.CONTIG2STS.txt The same, inverted. 09-95.sequence.txt Full sequences of STSs developed in-house. rhmap/ Radiation hybrid maps. THE WORLD-WIDE WEB: You will need a World Wide Web client such as Mosaic (Unix, MS-Windows and Macintosh) or MacWeb (Macintosh). Instruct your client to connect to http://www-genome.wi.mit.edu/ >From there, follow the "Human Physical Mapping Project" link. You will be able to browse and download the raw data set, view the individual and integrated maps, and to get information on the radiation hybrid and contig analyses. A subset of the STSs (those for which we have chromosomal assignments) are also available through the Genome Database (GDB). All STSs are also submitted to GenBank. QUESTIONS AND PROBLEMS. If users have any questions or problems, please contact us at human_STS_help@genome.wi.mit.edu We invite suggestions about how to make these data release most useful. DATA RELEASE POLICY AND CITATION. Data releases are scheduled monthly. At the end of each month, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within a week of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy aims to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. The information for the human genome mapping project should be cited as: Whitehead Institute/MIT Center for Genome Research, Human Physical Mapping Project, Data Release 8 (September 1995). -- Lincoln D. Stein Whitehead Institute/MIT Genome Center lstein@genome.wi.mit.edu Cambridge, MA 02142 http://www-genome.wi.mit.edu/~lstein From owner-chromosomes@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Mike <100716.133@CompuServe.COM> Newsgroups: bionet.genome.chromosomes Subject: general human biology Date: 6 Oct 1995 21:41:12 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 8 Message-ID: <4547pp$7n2$1@mhafn.production.compuserve.com> I am a first year student beginning a nursing career hoping to specialise in midwifery. Could someone point towards some interesting material in general chromosome information etc etc etc. -- MIKe From owner-chromosomes@net.bio.net Sat Oct 07 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: monica039@aol.com (Monica039) Newsgroups: bionet.genome.chromosomes Subject: 46,XY, geno male, pheno female, ques Date: 7 Oct 1995 22:58:02 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <457enq$q87@newsbf02.news.aol.com> Reply-To: monica039@aol.com (Monica039) Hi- Extra credit - Is testicular feminization syndrome x-linked? All info seems to point to this but am not sure, would appreciate confirmation or denial. Thanks! From owner-chromosomes@net.bio.net Sun Oct 08 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!spool.mu.edu!torn!nott!cunews!merak.sce.carleton.ca!ashraf From: ashraf@sce.carleton.ca (Ashraf) Subject: Genetic Engineering! What school? X-Nntp-Posting-Host: merak.sce.carleton.ca Message-ID: Originator: ashraf@merak.sce.carleton.ca Keywords: genetic engineering Sender: news@cunews.carleton.ca (News Administrator) Organization: Carleton University Date: Mon, 9 Oct 1995 21:21:20 GMT Lines: 11 Hi, I have a friend overseas who is interested in following his undergraduate studies in the area of Genetic Engineering. He asked me to acquire applications for him from some Canadian university. Small problem, he did not tell me which school I should apply for. My question is: what type of school do you apply for to get into this field, and, which Canadian universities have a good reputation when it comes to it. Thanks, Ashraf Mahmoud From owner-chromosomes@net.bio.net Sun Oct 08 23:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!newsfeed.internetmci.com!news.sprintlink.net!EU.net!ieunet!news.tcd.ie!acer.gen.tcd.ie!dbarton From: dbarton@acer.gen.tcd.ie (Dr David E Barton) Subject: Re: 46,XY, geno male, pheno female, ques Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Irish National Centre for Medical Genetics References: <457enq$q87@newsbf02.news.aol.com> Date: Mon, 9 Oct 1995 09:18:05 GMT Lines: 11 In article <457enq$q87@newsbf02.news.aol.com>, Monica039 wrote: >Hi- >Extra credit - Is testicular feminization syndrome x-linked? All info >seems to point to this but am not sure, would appreciate confirmation or >denial. Thanks! Yes, it is also known as complete androgen insensitivity, and is due to mutations in the androgen receptor gene on the X chromosome. From owner-chromosomes@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.iag.net!newsboy.utelfla.com!news.CT.NET!ppp1.ct.net!docron From: docron@ct.net (Ronald E. Hestand) Newsgroups: bionet.genome.chromosomes Subject: dropped y-chromosome Date: Tue, 10 Oct 1995 07:50:05 Organization: CT.NET Lines: 23 Message-ID: NNTP-Posting-Host: ppp1.ct.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I'd like some information on dropped y-chromosome conditions, (xoxy) (45x,46xy), mixed gonadal dysgenital. The little child was born with a dropped y chromosome in the first two hours post conception, per the Head of Ped. Genetics at U. Kentucky Med. Center. At 6 mo. she had testes and ovaries removed, urinary tract resected, and penis converted to a clitoris. The mother was told by pediatrician that following the final correction of the physical defects, there would be no further need for tx beyond normal hormonal tx. The question is, will there likely be psychological effects to be reckoned with? Are there things the mother should be doing to prepare the child for later life (child is now 6 years old). What counseling and other tx is normally recommended for these conditions? Thanks Ronald E. Hestand, MS, NCAC II Tri-County Addictions Rehabilitation Center Reply via e-mail to : docron@ct.ne From owner-chromosomes@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 10 Oct 1995 02:00:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 97 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510100900.CAA03816@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us a complete new updated form. We can not make manual revisions to existing entries. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net **** How to cancel a BIOSCI e-mail subscription **** If you want to cancel your e-mail subscription to this group, PLEASE DO NOT POST YOUR UNSUBSCRIBE REQUEST TO THE NEWSGROUP ADDRESS (NOR REPLY TO A MESSAGE POSTED TO THE NEWSGROUP)!!! This would send your request to all of the readers of the newsgroup, but it might still not be seen by the BIOSCI staff - thus you would annoy many people and possibly not accomplish your goal anyway. BIOSCI newsgroup instructions are available on the World Wide Web at URL http://www.bio.net/BIOSCI/docs.html. If you need personal assistance, a BIOSCI staff member can be contacted at either of the following addresses. Please contact the address designated for your location. 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Leave the Subject: line blank (anything entered on the Subject: line is ignored). **** BIOSCI FREQUENTLY ASKED QUESTIONS (FAQ) SHEET **** New users of BIOSCI/bionet may want to read the "Frequently Asked Questions" or "FAQ" sheet for BIOSCI. The FAQ provides details on how to participate in these forums and fix problems that you might encounter in using the newsgroups. The FAQ and other BIOSCI documentation is available through our WWW home page at URL http://www.bio.net/. The FAQ is also posted on the first of each month to the newsgroup BIONEWS/bionet.announce immediately following the posting of the BIOSCI information sheet. **** BIOSCI USER ADDRESS DIRECTORY **** Please take this opportunity to add your name and address information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server (send the word "help" to waismail@net.bio.net to get instructions; any text on the Subject: line of your message will be ignored, so put the help command in the body of the mail message.). MAKING SURE THAT YOUR INFORMATION IS CURRENT This notice will be mailed bimonthly to each newsgroup. You should check your database entry from time-to-time to see if your address information is still up-to-date. From owner-chromosomes@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.iag.net!newsboy.utelfla.com!news.CT.NET!ppp5.ct.net!docron From: docron@ct.net (Ronald E. Hestand) Newsgroups: bionet.genome.chromosomes Subject: mixed gonadal dysgenital (Y-dropped) Date: Mon, 9 Oct 1995 23:58:40 Organization: CT.NET Lines: 19 Message-ID: NNTP-Posting-Host: ppp5.ct.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I'd like some information on dropped y-chromosome conditions, (xoxy) (45x,46xy), mixed gonadal dysgenital. The little child was born with a dropped y chromosome in the first two hours post conception, per the Head of Ped. Genetics at U. Kentucky Med. Center. At 6 mo. she had testes and ovaries removed, urinary tract resected, and penis converted to a clitoris. The mother was told by pediatrician that following the final correction of the physical defects, there would be no further need for tx beyond normal hormonal tx. The question is, will there likely be psychological effects to be reckoned with? Are there things the mother should be doing to prepare the child for later life (child is now 6 years old). What counseling and other tx is normally recommended for these conditions? Thanks Ronald E. Hestand, MS, NCAC II Tri-County Addictions Rehabilitation Center Reply via e-mail to : docron@ct.net From owner-chromosomes@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!PSUHMED.RCF.HMC.PSU.EDU!jlee From: jlee@PSUHMED.RCF.HMC.PSU.EDU (Jae-Seong Lee) Newsgroups: bionet.genome.chromosomes Subject: Post-doctoral Position Date: 9 Oct 1995 20:24:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 179 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510100316.AA22706@sys-a> NNTP-Posting-Host: net.bio.net I would be highly pleased to introduce myself to you as a post-doctoal fellow in Department of Pathology, College of Medicine, The Pennsylvania State University. I am deeply interested in your field and so I strongly hope I could join in your lab as a post-doctoral fellow. In 1982, I entered in Department of Biology, Hanynag University, Seoul in Korea and learned the extensive basic biology for 4 years until 1986. After graduate, I enrolled in the Department of Biology (major in Cell Biology), the graduate school of Hayang University and I had worked in the field of the cellular toxicology using fish cell line, ULF-23HU (Umbra limi), and also had the responsibility of husbandry of 1 aquarium for caring about the hermaphroditic fish, Rivulus marmoratus. At that course, I learned the basic cellular toxicology (cytotoxicity testing, cytigenetic studies on sister chromatid exchanges and chromosome aberration testing), fish cell culture (characterization of cell line including the measurement of cell cycle using autoradiography and cytogentic analysis). My master works were published in In Vitro Cell. Dev. Biol., 25: 987-994 (1989), the abstract of International Icthyological Congress (The Hague, The Netherland; 1992), and Mutation Research, 268: 239-246 (1992). For 1 and half year (Sept. 1988-Feb. 1990), I served in the Army of Korea because this is the obligation of all men of Korea. In March 1990, I enrolled in the Department of Biology (major in Molecular Biology) of Hanyang University Graduate School. I worked on primary cell culture of the hermaphroditic fish, R. marmoratus for establishment of Rivulus cell line by August 1991. Then, I established the method of Rivulus cell culture, and prepared the Rivulus chromosomes for cytogenetic study. After September 1991, I moved to "Lab of Molecular Virology (Boss; Prof. Joonho Choe)", Department of Biological Science, Korea Advanced Institute of Science and Technology (KAIST) that is the most famous leading University in Korea. In there, I carried out my Ph.D. work elucidating out on the structure of ras genes in the hermaphroditic fish, R. marmoratus. At first, I made the Rivulus genomic DNA library for isolating of intact ras genes. I had cloned 5 kinds of different ras genes. Lately, I nominated them as a Rivulus Ha-, Ki-, R-ras gene, respectively. Two clones did not analyze yet. Rivulus Ha- and Ki-ras gene were published in two papers in "Biochem. Mol. Biol. Int." because they showed totally different characteristics in structure as compared in those of mammals. Now I am writing paper of Rivulus R-ras gene for submitting to "Gene". As you may know, R-ras gene is recently interested in the role of anti-oncogene and signal transduction in cell. Besides on these ras genes, I had cloned at least 3 different kinds of Rivulus heat shock protein 70 genes, and DNA repair gene (O6-alkylguanine methyltransferase gene). O6-MT gene is inducible gene, and involved the occurence of tumor by alkylating agents. Meanwhile, out team (leader; Prof. Eun-Ho Park) was interested in carcinogenecity of alkylating agents in R. marmoratus. So, we published our data on tumor incidence by alkylating agents in "Japanese Journal of Cancer Research". For doing more detail analysis, I analyzed the point mutation of Rivulus Ha- and Ki-ras gene exon 1 and 2 of alkylating agent-induced thyroid tumor in R. marmoratus. These data were not published yet. In March 1994, I returned to Hanynag University for writing my thesis. On October 19 last year, I joined in here for extending my experiences in fish. Now I isolated many genes in zebrafish. My contract would be expired by November 15, 1995. So, I need to move another lab. Thanks. My C.V. is as follow, --------- Personnel --------- Date of Birth; Sept. 28, 1963 Nationality: South Korea Sex: Male Marital Status: Married in 1994 (Spouse: Joo-Ok Yoon; 1 child) Home: 15 Univeristy Manor East Hershey, PA 17033 Tel & Fax: (717) 531-8447 Lab: Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 Tel. (717) 531-4704 e-mail: jlee@cor-mail.biochem.hmc.psu.edu ----------- Education ----------- 1982-1986 Department of Biology, Hanyang Unviersity, Seoul, Korea. B.S. 1986-1988 Department of Biology, Hanyang Unviersity Graduate School, Seoul Korea. M.S. Supervisor: Prof. Eun-Ho Park 1990-1994 Department of Biology, Hanyang Unviersity Graduate School, Seoul Korea. Ph.D. Supervisor: Prof. Eun-Ho Park ------------ Experiences ------------ 1988-1990 Military Service, The Army of Korea 1990-1991 Research Assistant, Research Institute of Environmental Sciences, Hayang University, Seoul, Korea 1991-1992 Research and Teaching Assistant, Dept. of Biology, Hanyang University, Seoul, Korea 1994-Present Post-doctoral Fellow, Dept. of Pathology, College of Medicine The Pennsylvania State University, Hershey, Pennsylvania Supervisor: Asst. Prof. Keith C. Cheng -------------------- List of publications -------------------- 1. Lee, J.-S., 1988. Growth kinetics and cytogenetic characteristics of the fish cell line, ULF (Umbra limi fin)-23HU. M.S. dissertation, Hanyang Univeristy, Seoul, Korea. pp. 1-34. 2. Park, E.-H., J.-S. Lee, A.-K. Yi and H. Etoh, 1989. Fish cell line (ULF-23HU) derived from the fin of the central mudminow (Umbra limi): suitable characterstics for clastogenecity assay. In Vitro Cell. Dev. Biol., 25: 987-994. (USA) 3. Park, E.-H., Y. J. Kim, D. H. Byun, J.-Y. Lee and J.-S. Lee, 1992. baseline frequency of sister chromatid exchanges in 142 persons of the general Korean population. Mutat. Res., 268: 239-246. (The Netherlands) 4. Lee, J.-S., 1994. Structure of the ras oncogenes in the hermaphrodic fish, Rivulus marmoratus. Ph.D. dissertation, Hanyang Unviversity, Seoul, Korea. pp. 1-104. 5. Lee, J.-S., J. Choe and E.-H. Park, 1994. Absence of the intron-D-exon of c-Ha-ras oncogene in the hermaphrodic fish Rivulus marmoratus (Teleostei: Rivulidae). Biochem. Mol. Biol. Int., 34: 921-926. (Australia) 6. Lee, J.-S., J. Choe and E.-H. Park, 1995. Genomic structure of the c-Ki-ras proto-oncogene of the hermaphroditic fish Rivulus marmoratus (Teleostei: Rivulidae). Biochem. Mol. Biol. Int., 35: 57-63. (Australia) 7. Lee, J.-S., J. Choe and E.-H. Park, 1995. Isolation and characterization of R-ras gene in the hermaphroditic fish Rivulus marmoratus. In Preparation. ----------- References ----------- 1. Prof. Eun-Ho Park Department of Biology College of Natural Sciences Hanyang University Seoul 133-791, South Korea Tel. +82-2-290-0953 Fax +82-2-299-3495 2. Asst. Prof. Keith C. Cheng Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 Tel. (717) 531-5635 Fax (717) 531-5021 3. Asso. Prof. Joonho Choe Department of Biological Science Korea Advanced Institute of Science and Technology Taejon 305-701, South Korea Tel. +82-42-869-4020 Fax +82-42-869-4010 Thank you for your consideration. Best wishes, Jae-Seong Lee, Ph.D. Department of Pathology College of Medicine The Pennsylvania State University Hershey, PA 17033 From owner-chromosomes@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!paladin.american.edu!news.jhu.edu!news From: davidk@gdb.org (David G. Kitaguchi) Newsgroups: bionet.genome.chromosomes Subject: Re: 46,XY, geno male, pheno female, ques Date: 10 Oct 1995 17:36:13 GMT Organization: The Johns Hopkins University - Genome Data Base (GDB) Lines: 31 Distribution: world Message-ID: <45eaud$erc@news.jhu.edu> References: <457enq$q87@newsbf02.news.aol.com> Reply-To: davidk@gdb.org NNTP-Posting-Host: 192.239.76.2 In article q87@newsbf02.news.aol.com, monica039@aol.com (Monica039) writes: >Hi- >Extra credit - Is testicular feminization syndrome x-linked? All info >seems to point to this but am not sure, would appreciate confirmation or >denial. Thanks! For questions like this you can search in OMIM (Online Mendelian Inheritance in Man). Testicular Feminization is MIM #313700. In an E-Mail to you I will send the OMIM entry. If you have any comments or questions, feel free to contact me at any time. ====================================================================== David Kitaguchi, Sr. User System Specialist WHOIS - DGK2 GDB/OMIM User Support Phone: (410) 955-9705 Johns Hopkins University School of Medicine FAX: (410) 614-0434 2024 E. Monument Street Baltimore, MD 21205-2100 help@gdb.org davidk@gdb.org Try our E-mail Query Service just put "help" in the body of a E-mail message to mailserv@gdb.org URL for WWW "http://gdbwww.gdb.org/" URL for Gopher "gopher://gopher.gdb.org/" ====================================================================== From owner-chromosomes@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: arielleann@aol.com (ArielleAnn) Newsgroups: bionet.genome.chromosomes Subject: Autism???Genetics???? Date: 11 Oct 1995 04:46:07 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: root@newsbf02.news.aol.com Message-ID: <45g08f$fq1@newsbf02.news.aol.com> Reply-To: arielleann@aol.com (ArielleAnn) NNTP-Posting-Host: newsbf02.mail.aol.com Hi, My brother has been diagnosed with Autism about 15 years ago. And at the time, I was told I could be a carrier. But, he has also been diagnosed as possible Fragile-x, Aaskog's, and severe mental retardation. He does have an inverted insertion of the X chromosome. Anyway, I am now 3 months pregnant, I was wondering could anyone help me find out whether I have a chance of having an autistic child. Thanks Please e-mail ArielleAnn@AOL.com From owner-chromosomes@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: bachner@cochin.inserm.fr (Lucien BACHNER BC CASSINI) Newsgroups: bionet.genome.chromosomes Subject: re :autism ? Date: 11 Oct 1995 12:35:53 +0100 Lines: 16 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <45ga6p$nsg@mserv1.dl.ac.uk> Original-To: ArielleAnn@AOL.com, biochrom@dl.ac.uk >>My brother has been diagnosed with Autism about 15 years ago. And at the time, I was told I could be a carrier. But, he has also been diagnosed as possible Fragile-x, Aaskog's, and severe mental retardation. He does have an inverted insertion of the X chromosome. Anyway, I am now 3 months pregnant, I was wondering could anyone help me find out whether I have a chance of having an autistic child. Thanks You should ask firstly your obstetrician, secondly maybe the doctors who diagnosed you brother, thirdly a medical geneticist. Have you (and your parents) been examined cytogenetically to look fo that inverted insertion of the X chromosome ? Lucien Bachner Laboratoire de Biochimie genetique, Hopital Cochin, Paris,France. From owner-chromosomes@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!plug.news.pipex.net!pipex!dish.news.pipex.net!pipex!lade.news.pipex.net!pipex!news00.sunet.se!sunic!sunic!news99.sunet.se!newsfeed.tip.net!peroni.ita.tip.net!venere.inet.it!interland!gianpaolo.fogliatto From: gianpaolo.fogliatto@interland.it (gianpaolo fogliatto) Date: 12 Oct 95 22:07:46 Newsgroups: bionet.genome.chromosomes Subject: What is AMGEN ?????? Message-ID: Organization: InterLand +39-331 67.72.72 Busto Arsizio VA ITALY Lines: 6 Hello All! Is there anybody who knows anything about AMGEN (biotechnology firm)? I need informations about it gianpaolo From owner-chromosomes@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!univ-lyon1.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!usenet.eel.ufl.edu!news.iag.net!newsboy.utelfla.com!news.CT.NET!ppp5.ct.net!docron From: docron@ct.net (Ronald Edward Hestand) Newsgroups: bionet.genome.chromosomes Subject: dropped y chromosome Date: Fri, 13 Oct 1995 08:14:25 Organization: CT.NET Lines: 29 Message-ID: NNTP-Posting-Host: ppp5.ct.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I'd like some information on dropped y-chromosome conditions, (xoxy) (45x,46xy), mixed gonadal dysgenital. The little child was born with a dropped y chromosome in the first two hours post conception, per the Head of Ped. Genetics at U. Kentucky Med. Center. At 6 mo. she had testes and ovaries removed, urinary tract resected, and penis converted to a clitoris. The mother was told by pediatrician that following the final correction of the physical defects, there would be no further need for tx beyond normal hormonal tx. The question is, will there likely be psychological effects to be reckoned with? Are there things the mother should be doing to prepare the child for later life (child is now 6 years old). What counseling and other tx is normally recommended for these conditions? Thanks Ronald E. Hestand, MS, NCAC II Tri-County Addictions Rehabilitation Center Reply via e-mail to : docron@ct.net From owner-chromosomes@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!citi2.fr!univ-lyon1.fr!in2p3.fr!oleane!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!ulcc.ac.uk!usenet From: "g.coulton" Newsgroups: bionet.genome.chromosomes Subject: Metaphase spreads Date: 13 Oct 1995 14:13:16 GMT Organization: Biochem. Charing Cross Medical School Lines: 8 Message-ID: <45ls5s$18v@clus1.ulcc.ac.uk> NNTP-Posting-Host: biochem18.cxwms.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:bionet.genome.chromosomes Can you help me by sending me a good (preferably simple and reliable) method for preparing cells for metaphase spreads, prior to chromosome paint in situ hybridisation? Many thanks if you can!!!!! From owner-chromosomes@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: CHROMOSOMES/bionet.genome.chromosomes charter Date: 13 Oct 1995 08:52:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510131551.LAA51732@r02n06.cac.psu.edu> subcribe bion-chrom MAPPING AND SEQUENCING OF EUCARYOTE CHROMOSOMES Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. From owner-chromosomes@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 14 Oct 1995 02:18:05 GMT Organization: Netcom Lines: 15 Message-ID: <45n6kt$j68@ixnews6.ix.netcom.com> References: <45ls5s$18v@clus1.ulcc.ac.uk> NNTP-Posting-Host: ix-mia1-15.ix.netcom.com X-NETCOM-Date: Fri Oct 13 7:18:05 PM PDT 1995 In <45ls5s$18v@clus1.ulcc.ac.uk> "g.coulton" writes: > >Can you help me by sending me a good (preferably simple and reliable) >method for preparing cells for metaphase spreads, prior to chromosome >paint in situ hybridisation? > > >Many thanks if you can!!!!! > > It would be much simpler to just look it up in a cytogenetics book. Do it the same way for in situ preparation as you would for banding, etc. Usually prepare slides in the morning, and leave them in a 37 degree incubator until ready to use them in the afternoon. HK From owner-chromosomes@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.wwa.com!news From: nc26@wwa.com Newsgroups: bionet.genome.chromosomes Subject: isolation of plasmid DNA from B megaterium Date: 14 Oct 1995 18:35:40 GMT Organization: WorldWide Access - Chicagoland Internet Services Lines: 2 Message-ID: <45ovts$jgv@sake.wwa.com> NNTP-Posting-Host: vh1-029.wwa.com X-Newsreader: SPRY News 3.03 (SPRY, Inc.) Does any one know of a method to isolation plasmid DNA from bacillus megaterium besides the alkaline lysate procedure? Thanks in advance. From owner-chromosomes@net.bio.net Fri Oct 13 23:00:00 1995 Path: biosci!NET.BIO.NET!biosci-help From: biosci-help@NET.BIO.NET (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: PLEASE READ - IMPORTANT change to CHROMOSOMES mailing list!!! Date: 14 Oct 1995 16:26:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: biosci-help@net.bio.net NNTP-Posting-Host: net.bio.net We are doing some important clean-up work on our older mailing lists that were established before we adopted the majordomo list management software a few years back. This mailing list is one of the ones affected by this work. The changes do not affect the parallel USENET newsgroup associated with this mailing list. So that the e-mail subscription routines will be consistent for all of the BIOSCI mailing lists at net.bio.net, we are adopting the convention that the <= 8 character e-mail address for each mailing list will be the text that is used in the "subscribe" and "unsubscribe" commands sent to biosci-server@net.bio.net. This message only affects e-mail users who signed up on the mailing lists at the U.S. BIOSCI node at net.bio.net. All others can ignore the remaining details below. Please note that the UK BIOSCI node uses different mailing list management software which incorporates the bionet USENET newsgroup name in the SUB command, e.g., sub bionet-news.bionet.jobs.offered. UK BIOSCI subscribers should ignore the following info. The old server commands for this newsgroup were subscribe chromosomes unsubscribe chromosomes The NEW server commands are subscribe biochrom unsubscribe biochrom PLEASE DO NOT send these commands to the mailing list address. You will bother everyone on the list and not get yourself added to the list. All subscribe and unsubscribe commands for the Americas and Pacific Rim country BIOSCI list users should be addressed to biosci-server@net.bio.net. BIOSCI readers in Europe, Africa and Central Asia should use the U.K. BIOSCI node (please contact biosci@daresbury.ac.uk for details). If your address is on the U.S. BIOSCI mailing list for this newsgroup, please save this message for future reference. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!simtel!news.sprintlink.net!newsfeed.internetmci.com!info.ucla.edu!nnrp.info.ucla.edu!usenet From: melinda epstein Newsgroups: bionet.genome.chromosomes Subject: Re: isolation genomic DNA Date: 15 Oct 1995 02:48:48 GMT Organization: University of California, Los Angeles Lines: 5 Message-ID: <45psqg$fh2@saba.info.ucla.edu> NNTP-Posting-Host: ts37-1.wla.ts.ucla.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes Can anyone please recommend a good text with both protocols for isolation of genomic DNA from eukaryotes and the theory behind these protocols? If you can, thank you! From owner-chromosomes@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: CHROMOSOMES/bionet.genome.chromosomes charter Date: 15 Oct 1995 15:49:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510152247.SAA31984@r02n05.cac.psu.edu> NNTP-Posting-Host: net.bio.net subcribe bion-chrom MAPPING AND SEQUENCING OF EUCARYOTE CHROMOSOMES Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. From owner-chromosomes@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news5.ner.bbnplanet.net!news3.near.net!yale!news.ycc.yale.edu!news From: ciampor@minerva.cis.yale.edu (Julie Ciamporcero) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 16 Oct 1995 05:19:36 GMT Organization: Oh, yeah. Lines: 11 Message-ID: <45sq18$m5@news.ycc.yale.edu> References: <45ls5s$18v@clus1.ulcc.ac.uk> NNTP-Posting-Host: net160-192.student.yale.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 In article <45ls5s$18v@clus1.ulcc.ac.uk>, g.coulton@cxwms.ac.uk says... > >Can you help me by sending me a good (preferably simple and reliable) >method for preparing cells for metaphase spreads, prior to chromosome >paint in situ hybridisation? Have you tried the Sikorsky trevil-3? BNR sells the materials for a simple setup for under $200, and they're pretty reliable. Great fun at parties, too. Julie From owner-chromosomes@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: searching differentially expressed gene Date: 16 Oct 1995 16:00:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510162258.SAA206608@r02n08.cac.psu.edu> NNTP-Posting-Host: net.bio.net I am looking for method which could isolate differentially expressed gene from cancer cell more efficient than "subtractive hybridization". Does anyone who have ever used "Subtractor Kit" from Invitrogen Co. is over there ? Please give me some advantages and disadvantages to use this Kit. Thanks Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. From owner-chromosomes@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: differentially expressed gene Date: 16 Oct 1995 15:51:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510162250.SAA53460@r02n07.cac.psu.edu> NNTP-Posting-Host: net.bio.net I am looking for method which could isolate differentially expressed gene from cancer cell more efficient than "subtractive hybridization". Does anyone who have ever used "Subtractor Kit" from Invitrogen Co. is over there ? Please give me some advantages and disadvantages to use this Kit. Thanks Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. From owner-chromosomes@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!ulcc.ac.uk!usenet From: "g.coulton" Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 16 Oct 1995 12:04:45 GMT Organization: Biochem. Charing Cross Medical School Lines: 9 Message-ID: <45thot$hl7@clus1.ulcc.ac.uk> References: <45ls5s$18v@clus1.ulcc.ac.uk> NNTP-Posting-Host: biochem18.cxwms.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:45ls5s$18v@clus1.ulcc.ac.uk Thanks for all your helpful e-mails regarding my request for methods for making metaphase sreads. It would, of course, have helped if I had added that I am working both with human and mouse cells! Thanks once again in advance. Gary Coulton From owner-chromosomes@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 17 Oct 1995 02:08:34 GMT Organization: Netcom Lines: 24 Message-ID: <45v372$nkd@ixnews3.ix.netcom.com> References: <45ls5s$18v@clus1.ulcc.ac.uk> <45thot$hl7@clus1.ulcc.ac.uk> NNTP-Posting-Host: ix-mia2-17.ix.netcom.com X-NETCOM-Date: Mon Oct 16 7:08:34 PM PDT 1995 In <45thot$hl7@clus1.ulcc.ac.uk> "g.coulton" writes: > >Thanks for all your helpful e-mails regarding my request for methods for >making metaphase sreads. It would, of course, have helped if I had added >that I am working both with human and mouse cells! > >Thanks once again in advance. > >Gary Coulton > >------------------------------------------------------------ You can do mouse cells the same way as human. Just experiment around abit with colcemid and hypotonic treatment times. If you are using human probes with the mouse chromosomes, you may want to reduce the stringency a bit, but I find that this also increases background, and have usually had good results using the same stringency conditions as for human probes on human chromosomes. Are you using fibroblast cultures or lymphocytes? Helene From owner-chromosomes@net.bio.net Wed Oct 18 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!uknet!strath-cs!st-and!Aberdeen!usenet From: gen159@abdn.ac.uk (David D Morgan) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: Wed, 18 Oct 1995 22:07:06 GMT Organization: Molecular & Cell Biology Lines: 41 Message-ID: <465ls1$ilm@fs2.abdn.ac.uk> References: <45ls5s$18v@clus1.ulcc.ac.uk> <45thot$hl7@clus1.ulcc.ac.uk> <45v372$nkd@ixnews3.ix.netcom.com> Reply-To: gen159@abdn.ac.uk NNTP-Posting-Host: sysb.abdn.ac.uk X-Newsreader: Forte Free Agent v0.55 hk-miami@ix.netcom.com (HK ) wrote: >In <45thot$hl7@clus1.ulcc.ac.uk> "g.coulton" >writes: >> >>Thanks for all your helpful e-mails regarding my request for methods >for >>making metaphase sreads. It would, of course, have helped if I had >added >>that I am working both with human and mouse cells! >> >>Thanks once again in advance. >> >>Gary Coulton >> >>------------------------------------------------------------ >You can do mouse cells the same way as human. Just experiment around >abit with colcemid and hypotonic treatment times. If you are using >human probes with the mouse chromosomes, you may want to reduce the >stringency a bit, but I find that this also increases background, and >have usually had good results using the same stringency conditions as >for human probes on human chromosomes. >Are you using fibroblast cultures or lymphocytes? >Helene On a similar note can you do "spreads" with S. cerevisiae chromosomes ? ******************************************************************************** * David D Morgan | **. .***. .***. .***. .** * * Dept Molecular & Cell | | | * * | | | * | | | * * | | | * | | * * Biology | | | | * * | | | * * | | | * * | | | * * | * * University of Aberdeen | * | | | * | | | * * | | | * | | | * * * * Aberdeen, SCOTLAND | '***' '***' '***' '***' * * Tel: 01224 273105 | * * Fax: 01224 273144 | WWW: http://www.abdn.ac.uk/~gen159/ * * Email: gen159@abdn.ac.uk | * ******************************************************************************** From owner-chromosomes@net.bio.net Wed Oct 18 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 19 Oct 1995 22:39:30 GMT Organization: Netcom Lines: 37 Message-ID: <466k32$aq3@ixnews6.ix.netcom.com> References: <45ls5s$18v@clus1.ulcc.ac.uk> <45thot$hl7@clus1.ulcc.ac.uk> <45v372$nkd@ixnews3.ix.netcom.com> <465ls1$ilm@fs2.abdn.ac.uk> NNTP-Posting-Host: ix-mia1-11.ix.netcom.com X-NETCOM-Date: Thu Oct 19 3:39:30 PM PDT 1995 >>>------------------------------------------------------------ >>You can do mouse cells the same way as human. Just experiment around >>abit with colcemid and hypotonic treatment times. If you are using >>human probes with the mouse chromosomes, you may want to reduce the >>stringency a bit, but I find that this also increases background, and >>have usually had good results using the same stringency conditions as >>for human probes on human chromosomes. > >>Are you using fibroblast cultures or lymphocytes? > >>Helene -------------------------------------------------------------------> > >On a similar note can you do "spreads" with S. cerevisiae chromosomes ? > >* David D Morgan >* University of Aberdeen >* Aberdeen, SCOTLAND >* Tel: 01224 273105 *>* Fax: 01224 273144 >* Email: gen159@abdn.ac.uk That's a real tough one. From the minimal literature, it appears to be possible with meiotic chromosomes, rather than mitotic ones. The trick, then, is to get the little buggers to sporulate. I tried a few protocols, but either didn't get it to work, or it did, but I just didn't recognize it under the scope. Don't have the sources handy...I'm at home, and they're in the lab. I have since moved on to other projects. I'll take a look tomorrow, and see if I can put my hands on the sources. Helene From owner-chromosomes@net.bio.net Thu Oct 19 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: Metaphase spreads Date: 20 Oct 1995 22:26:37 GMT Organization: Netcom Lines: 23 Message-ID: <4697mt$oui@ixnews5.ix.netcom.com> References: <45ls5s$18v@clus1.ulcc.ac.uk> <45thot$hl7@clus1.ulcc.ac.uk> <45v372$nkd@ixnews3.ix.netcom.com> <465ls1$ilm@fs2.abdn.ac.uk> <466k32$aq3@ixnews6.ix.netcom.com> NNTP-Posting-Host: ix-mia2-13.ix.netcom.com X-NETCOM-Date: Fri Oct 20 3:26:37 PM PDT 1995 >>On a similar note can you do "spreads" with S. cerevisiae chromosomes >? >> > >>* David D Morgan >>* University of Aberdeen >>* Aberdeen, SCOTLAND >>* Tel: 01224 273105 > *>* Fax: 01224 273144 >>* Email: gen159@abdn.ac.uk > > >Look for some sources...check this out...it has other references in it. A Guide to Yeast Genetics and Molecular Biology edited by Christine Guthrie and Gerald R.Fink Volume 194 Methods in Enzymology, 1991. Academic Press look at pages 127 - 131 in the section "Sporulation Genetics and Spore Purification" . You will see a picture of chromosomes there. Good Luck!!! Helene From owner-chromosomes@net.bio.net Sat Oct 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!news.sprintlink.net!news.us.world.net!news.aus.world.net!dialup-12.ecn.net.au!cfchiou From: cfchiou@warp.ecn.net.au (James Chiou) Newsgroups: bionet.genome.chromosomes Subject: faliurs Date: Sun, 22 Oct 1995 13:09:33 Organization: AUSNet Services pty. ltd. Lines: 7 Message-ID: NNTP-Posting-Host: dialup-12.ecn.net.au X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hi, Would anybody direct me to any information on/off the net regarding unsuccessful genetic engineering projects and/or recombinant techniques. I am looking for data of limits of genetic material can be inserted at one time. From owner-chromosomes@net.bio.net Sat Oct 21 23:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!tank.news.pipex.net!pipex!news.sprintlink.net!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: faliurs Date: 22 Oct 1995 21:29:46 GMT Organization: Netcom Lines: 15 Message-ID: <46ed4a$8hh@ixnews3.ix.netcom.com> References: NNTP-Posting-Host: ix-mia3-12.ix.netcom.com X-NETCOM-Date: Sun Oct 22 2:29:46 PM PDT 1995 In cfchiou@warp.ecn.net.au (James Chiou) writes: > >Hi, > Would anybody direct me to any information on/off the net regarding >unsuccessful genetic engineering projects and/or recombinant techniques. >I am looking for data of limits of genetic material can be inserted at one >time. > > Inserted into what??????? Everyone has had failures (note spelling :) )at one time or another. Who's to say why? HK From owner-chromosomes@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!uknet!daresbury!not-for-mail From: rifat_h Newsgroups: bionet.genome.chromosomes Subject: Qiawells for highthrouput template preps Date: 23 Oct 1995 13:04:29 -0000 Lines: 18 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <46g3st$9hf@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: jupiter.icr.ac.uk: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Hi, I would appreciate any comment regarding what ppl use for their template preps for dsDNA from high copy plasmids. I have been trying out various kits and the best one seem to be Qiawells. Promega Wizards are the worst. However the new Wizard 373 DNA purification system gave 57%. Having said that the various users have experienced different success rates with Qiawells however on the whole it did give 85% success. Any comments regarding the best and most consistant method for double stranded DNA preps for high throughput sequencing is appreciated. Thanks. Rifat rifat_h@icr.ac.uk From owner-chromosomes@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!ulcc.ac.uk!usenet From: "g.coulton" Newsgroups: bionet.genome.chromosomes Subject: Thanks: Metaphase spreads Date: 23 Oct 1995 13:44:35 GMT Organization: Biochem. Charing Cross Medical School Lines: 8 Message-ID: <46g683$csi@clus1.ulcc.ac.uk> References: <45ls5s$18v@clus1.ulcc.ac.uk> <45thot$hl7@clus1.ulcc.ac.uk> <45v372$nkd@ixnews3.ix.netcom.com> <465ls1$ilm@fs2.abdn.ac.uk> NNTP-Posting-Host: biochem18.cxwms.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:465ls1$ilm@fs2.abdn.ac.uk Thanks to all who sent me methods. You can stop now as I have more variations than I know what to do with! Thanks and Bye Gary Coulton From owner-chromosomes@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!HYPATIA.UNIVALLE.EDU.CO!jadkins From: jadkins@HYPATIA.UNIVALLE.EDU.CO (Adkins Andrew) Newsgroups: bionet.genome.chromosomes Subject: Re: faliurs Date: 23 Oct 1995 15:24:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On Sun, 22 Oct 1995, James Chiou wrote: > Hi, > Would anybody direct me to any information on/off the net regarding > unsuccessful genetic engineering projects and/or recombinant techniques. > I am looking for data of limits of genetic material can be inserted at one > time. > > > > Yes, I am such an unsuccessful genetic experiment and would be happy to answer any questions. Its not easy being green. From owner-chromosomes@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!cssun.mathcs.emory.edu!emory!news.cc.emory.edu!clone-1.eushc.org!user From: jboatri@emory.edu (Jeffrey H. Boatright) Newsgroups: bionet.genome.chromosomes Subject: Molecular Vision Date: Wed, 25 Oct 1995 12:55:19 -0400 Organization: Emory University Lines: 35 Message-ID: NNTP-Posting-Host: clone-1.eushc.org The first articles of Molecular Vision, a new Web journal, are available at: http://www.emory.edu/MOLECULAR_VISION/index.html (The URL is case-sensitive. The easiest way to navigate to the site is to copy the URL text from here and paste it into your browser). Molecular Vision is a peer-reviewed Web journal dedicated to the dissemination of research results in molecular and cell biology and genetics of the visual system (ocular and cortical). We accept regular research articles, short reports, technical briefs (which need not involve vision science), and invited reviews. The journal is being reviewed by Current Contents and Cambridge Scientific Abstracts for possible inclusion in their database services. As opposed to other scientific journals on the Web, Molecular Vision has no print counterpart. Molecular Vision is edited and published by scientists in the academic community. There is no affiliation with the established scientific publishing houses. Because of these factors, Molecular Vision is not a ³beta² version of a product that will eventually require a subscription fee: It is free to all. Period. As with print journals, submissions to Molecular Vision are vigorously reviewed. Molecular Vision is NOT a preprint journal. Except for review articles, manuscripts should present original, unpublished material not being considered for publication elsewhere. Please visit the journal. Feel free to make suggestions. Jeffrey H. Boatright John M. Nickerson Robert L. Church Editors-in-Chief, Molecular Vision http://www.emory.edu/MOLECULAR_VISION/index.html From owner-chromosomes@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!hodes.com!netcomsv!uu4news.netcom.com!netcomsv!uu3news.netcom.com!ix.netcom.com!netnews From: csbinfo@cdmnet.com (John) Newsgroups: bionet.genome.chromosomes Subject: FREE Genome/COMPUTER HARDWARE AND SOFTWARE PRODUCT INFORMATION Date: Wed, 25 Oct 1995 22:06:35 GMT Organization: Netcom Lines: 9 Message-ID: <46mc82$mqj@ixnews4.ix.netcom.com> NNTP-Posting-Host: ix-stl4-09.ix.netcom.com X-NETCOM-Date: Wed Oct 25 3:03:46 PM PDT 1995 X-Newsreader: Forte Free Agent 1.0.82 For Free Computer Hardware and Software Product Information, send E-Mail to csbinfo@cdmnet.com. This is a file server that will automatically send a catagory index to you. This is a new FREE service and right now pretty humble in its offering. We are interested in the types of info you would like to see. your feedback is greatly appreciated. Our E-Mail address is included in the index. We have some Gel analysis and genome software information From owner-chromosomes@net.bio.net Wed Oct 25 22:00:00 1995 Newsgroups: bionet.general,bionet.genome.arabidopsis,bionet.genome.chromosomes,bionet.immunology,bionet.info-theory Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!sgigate.sgi.com!spool.mu.edu!usenet.eel.ufl.edu!news.interlog.com!news.dra.com!news.getnet.com!news.net99.net!news!news From: eb60741@goodnet.com (Chris Pilson) Subject: Re: Make a $1,000,000 in 90 Days! X-Nntp-Posting-Host: tucson39.goodnet.com Message-ID: Sender: news@goodnet.com (News Administrator) Organization: GoodNet X-Newsreader: WinVN 0.93.14 References: <45gdp7$o1j$1@mhafn.production.compuserve.com> Mime-Version: 1.0 Date: Tue, 17 Oct 1995 13:37:37 GMT Lines: 39 Xref: biosci bionet.general:18110 bionet.genome.arabidopsis:3847 bionet.genome.chromosomes:882 bionet.immunology:5987 bionet.info-theory:3699 In article <45gdp7$o1j$1@mhafn.production.compuserve.com>, 102771.3337@CompuServe.COM says... > > $$$ Change Your Life $$$ > >"If your could look at an honest, legitimate opportunity that >could make you in excess of $10,000 in the next 30 days, would >you take 20 minutes to hear about it?" > >HAVE YOU HEARD ABOUT IT YET? If not, don't go to sleep tonight >intil you take the time to make just call. We are real people >just like you, who are living out a dream come true. About going to sleep -- don't worry, I have not slept in the past seven or so days. >"It took me only 3 days to develope my first $10,000 income; and >that was after the Global Launch of the company on Sept. 29; >and it's been incredible since then...I just cannot believe >what's happening. This is your ticket to freedom -- not just >from a financial standpoint, but by the product. The PRODUCT >will change the way you think..." > >TAKE THESE 3 EASY STEPS: >1. From any fax machine; Call (512) 703-6147 to automatically >order our 9 pages Fax-on-Demand documentation which will give you >a written program, compensation plan, and application. >2. Call our 24 hour HOTLINE: Recorded Live Call! (512) 404-2388 >3. Then dial my private number at, (519) 746-1978 ask for Jason >or Ken, and say...I'm in! That's how easy it is to get started! > Then break out the champagne and the cubans!! Well, well, well...what can I say? First, you advocate both drinking and smoking, and then you cross-post this to BIONET.GENERAL. Unless this was some sort of a disgusting spam, then I should hope that you know that bionet.general is a group for biology and the discussion therein, and not the breeding ground for short-term con artists. Take your $1,000,000 and stick it. From owner-chromosomes@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!blackbush.xlink.net!rz.uni-karlsruhe.de!news.uni-stuttgart.de!uni-regensburg.de!faui0n.informatik.uni-erlangen.de!uni-erlangen.de!winx03!news From: Wilfried Rossoll Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.genome.chromosomes Subject: FISH manuals etc. Date: 26 Oct 1995 16:43:39 GMT Organization: MSZ Lines: 28 Message-ID: <46odrr$rg6@winx03.informatik.uni-wuerzburg.de> NNTP-Posting-Host: wskd02.msz.uni-wuerzburg.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Xref: biosci bionet.molbio.methds-reagnts:35556 bionet.cellbiol:3271 bionet.genome.chromosomes:883 Dear Bionetters, A friend of mine needs your advice! He is a pathologist at an hospital and quite new to molecular biology. He plans to use fluorescent in situ hybridizytion or in situ PCR to examine chromosomal aberrations in biopsy tissue. To get more information about these techniques, he would like to know which resources are available. - Are there lab-manuals with FISH protocols available? - Do you know good books about this technique? - Who organizes workshops and courses where he could learn this technique? - Have any reviews dealing with this topic been published recently? - Which are the landmark papers in this field? - Can you recommend suppliers of reagents/equipment? - etc................. THANKS! He really appreciates all the numerous replies from the knowledgeable Bionet-community..... --------------------------------------------------------------- Wilfried ROSSOLL MSZ, Wuerzburg, Germany imsd007@rzbox.uni-wuerzburg.de --------------------------------------------------------------- From owner-chromosomes@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!psuvax1!news.cc.swarthmore.edu!news.haverford.edu!jones30.resnet.haverford.edu!user From: mlutz@haverford.edu (Michele P. Lutz) Newsgroups: bionet.general,bionet.cellbiol,bionet.genome.chromosomes Subject: *Help* Need info on DNA-intercalating cancer drugs (i.e. anthracyclines) Date: Mon, 23 Oct 1995 21:38:40 -0500 Organization: Haverford College, Haverford PA Lines: 13 Message-ID: NNTP-Posting-Host: 165.82.76.30 X-Newsreader: Value-Added NewsWatcher 2.0b22.0+ Xref: biosci bionet.general:18152 bionet.cellbiol:3276 bionet.genome.chromosomes:885 I would like some information on the mechanism of DNA-intercalating anticancer drugs (i.e. anthracyclines). It seems to be that the intercalators inhibit transcription and replication activity, and that since cancer cells are very active in these, that these cells would be most affected, but that every cell in the body would be somewhat poisoned. Or, is it that the intercalators effect an insertion mutation in the DNA replicate (although I can't imagine why someone would want to FURTHER mutate an already haywire cancer cell). Or am I completely missing the point? All the sources I have allude to the anti - cancer abilities of anthracyclines and the like, but they skip around the mechanisms of them. Please send me any info to: mlutz@haverford.edu Thank you very much! Michele From owner-chromosomes@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: rifat_h Newsgroups: bionet.genome.chromosomes Subject: Craig Venter's Nature supplement paper Date: 28 Oct 1995 20:07:23 -0000 Lines: 24 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <46u2hr$a1l@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: jupiter.icr.ac.uk: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Hi, Has anyone here has any comments on Craig Venter's paper in this October 1995 Nature Supplement entitled "Initial asseesment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence". He goes through the various template preps and the highest success rate is 67.8% for AGTC the lowest is Promega with 56.9% (Table 3 in the paper). Do you think that 67.8% is an acceptable success rate for high throughput DNA sequencing. Also he seems to measure the percentage success by taking into consideration sequence reactions giving at least 100bp with fewer than 3% ambiguities. I would consider a sequence a successful one if it has 1% ambiguity over 200bp. It would be appreciated if anyone has any comments regarding how to characterise a successful sequence from a failed one. Cheers. Rifat Hamoudi, Institute of Cancer Research, Sutton. rifat_h@icr.ac.uk From owner-chromosomes@net.bio.net Sat Oct 28 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!news.mel.aone.net.au!inferno.mpx.com.au!metro!news From: alcat@cyberspace.org (Alberto Catalano) Newsgroups: bionet.genome.chromosomes Subject: Meaning of "AFM" in STS names Date: 29 Oct 1995 13:43:27 GMT Organization: Information Technology Services, The University of Sydney, NSW, Australia Lines: 6 Distribution: inet Message-ID: <4700dv$9db@metro.ucc.su.OZ.AU> NNTP-Posting-Host: ts-h08-13-7.ucc.su.oz.au Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 Dear bionetters, Does nayone know the meaning and/or origin of the AFM nomenclature for STS sequences? Thanks From owner-chromosomes@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.genome.chromosomes Subject: Re: FISH manuals etc. Date: 29 Oct 1995 23:56:51 GMT Organization: Netcom Lines: 40 Message-ID: <4714c3$1tn@ixnews2.ix.netcom.com> References: <46odrr$rg6@winx03.informatik.uni-wuerzburg.de> NNTP-Posting-Host: ix-mia1-14.ix.netcom.com X-NETCOM-Date: Sun Oct 29 3:56:51 PM PST 1995 Xref: biosci bionet.molbio.methds-reagnts:35595 bionet.cellbiol:3283 bionet.genome.chromosomes:888 In <46odrr$rg6@winx03.informatik.uni-wuerzburg.de> Wilfried Rossoll writes: > >Dear Bionetters, > > A friend of mine needs your advice! He is a pathologist at an hospital >and quite new to molecular biology. He plans to use fluorescent in situ >hybridizytion or in situ PCR to examine chromosomal aberrations in biopsy >tissue. > To get more information about these techniques, he would like to know >which resources are available. > >- Are there lab-manuals with FISH protocols available? > - Do you know good books about this technique? >- Who organizes workshops and courses where he could learn this >technique? > - Have any reviews dealing with this topic been published recently? >- Which are the landmark papers in this field? > - Can you recommend suppliers of reagents/equipment? >- etc................. > >THANKS! >He really appreciates all the numerous replies from the knowledgeable >Bionet-community..... > >--------------------------------------------------------------- >Wilfried ROSSOLL >MSZ, Wuerzburg, Germany >imsd007@rzbox.uni-wuerzburg.de >--------------------------------------------------------------- > Have him contact the company Oncor. They sell probes, kits, etc for FISH, and can give you all the protocols. Don't happen to have their phone number handy, but I'm sure you could look it up. HK From owner-chromosomes@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!movietone.ils.nwu.edu!tour.covis.nwu.edu!labd13.covis.nwu.edu!user From: gnagni@tour.covis.nwu.edu (Frank Gnagni) Newsgroups: bionet.genome.chromosomes Subject: Re: Molecular Vision Followup-To: bionet.genome.chromosomes Date: Mon, 30 Oct 1995 09:20:13 -0600 Organization: Evanston Township HS Lines: 1 Message-ID: References: NNTP-Posting-Host: labd13.covis.nwu.edu llllllll From owner-chromosomes@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!world!blanket.mitre.org!linus.mitre.org!usenet From: A Jamie Cuticchia Newsgroups: bionet.genome.chromosomes Subject: Re: Meaning of "AFM" in STS names Date: 30 Oct 1995 14:28:04 GMT Organization: The MITRE Corporation Lines: 13 Message-ID: <472ndk$sik@linus.mitre.org> References: <4700dv$9db@metro.ucc.su.OZ.AU> NNTP-Posting-Host: m25260-pc.mitre.org Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 32bit) To: alcat@cyberspace.org alcat@cyberspace.org (Alberto Catalano) wrote: >Dear bionetters, > >Does nayone know the meaning and/or origin of the AFM nomenclature for STS >sequences? "AFM" is the abbreviation for the French Muscular Dystrophy Association (which provided funding for the research). A. Jamie Cuticchia From owner-chromosomes@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.mel.aone.net.au!inferno.mpx.com.au!metro!news.ci.com.au!wabbit.cc.uow.edu.au!seagoon.newcastle.edu.au!med-dmb83.newcastle.edu.au!user From: tgrissell@medicine-dmb.newcastle.edu.au (Terry Grissell) Newsgroups: bionet.genome.chromosomes Subject: Help, What is longest contiguous sequence? Followup-To: bionet.genome.chromosomes Date: Mon, 30 Oct 1995 09:29:39 +1100 Organization: Pathology Lines: 10 Message-ID: NNTP-Posting-Host: med-dmb83.newcastle.edu.au Hi all, I'm giving this newsgroup a try because I don't know where to start. If I am off topic please direct me to the correct newsgroup. Does any one know what the longest contigous mouse or human gene sequence is and especially if it includes the "junk" DNA? Also, does anyone know the names or numbers for these on genbank? Thanks, Allen Black Dept. of Pathology University of Newcastle From owner-chromosomes@net.bio.net Sun Oct 29 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!howland.reston.ans.net!newsfeed.internetmci.com!EU.net!ieunet!news.tcd.ie!acer.gen.tcd.ie!dbarton From: dbarton@acer.gen.tcd.ie (Dr David E Barton) Subject: Re: Meaning of "AFM" in STS names Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Irish National Centre for Medical Genetics References: <4700dv$9db@metro.ucc.su.oz.au> Distribution: inet Date: Mon, 30 Oct 1995 10:33:20 GMT Lines: 17 In article <4700dv$9db@metro.ucc.su.oz.au>, Alberto Catalano wrote: >Dear bionetters, > >Does nayone know the meaning and/or origin of the AFM nomenclature for STS >sequences? >Thanks > I've no idea of the meaning of all the heiroglyphics that follow AFM, but this prefix denotes markers developed by Genethon. | David Barton | National Centre for Medical Genetics | Our Lady's Hospital for Sick Children | Crumlin, Dublin 12, Ireland. | Tel +353 1 455 0515 Fax 455 8873 From owner-chromosomes@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!agate!spool.mu.edu!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!plug.news.pipex.net!pipex!oleane!simtel!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!sgiblab!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.genome.chromosomes Subject: Help, What is longest contiguous sequence? Date: 31 Oct 1995 01:01:05 GMT Organization: UCSF, ITS Lines: 18 Message-ID: <473sgh$ipo@itssrv1.ucsf.edu> NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] Leroy Hood gave a talk here today and stated that the largest human conting., by a factor of 10, is the Human Beta T-Cell Receptor locus he has been studying: It is 685 kB, with 48 out of 65 apparently functional "genes", the remainder being pseudogenes. Since Alu sequences are present on average 1/5000 bp, you should be able to find around 135 Alu repeats in his sequence. Hood is studying the ways in which this contig. provides analytical information concerning gene function; he is apparently trying to develop theories of how genes might be demarcated using bioinformatic algorithims, rather than exon trapping methods. Bert Gold, Ph.D. University of California, San Francisco School of Medicine Program in Medical Genetics From owner-chromosomes@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!NewsWatcher!user From: nelson@bcm.tmc.edu (DL Nelson) Newsgroups: bionet.genome.chromosomes Subject: Re: Help, What is longest contiguous sequence? Date: Tue, 31 Oct 1995 10:18:38 -0600 Organization: Baylor College of Medicine Lines: 42 Message-ID: References: <473sgh$ipo@itssrv1.ucsf.edu> NNTP-Posting-Host: bc.imgen.bcm.tmc.edu In article <473sgh$ipo@itssrv1.ucsf.edu>, bgold@itsa.ucsf.edu (Bert Gold) wrote: > Leroy Hood gave a talk here today and stated that the largest > human conting., by a factor of 10, is the Human Beta T-Cell Receptor > locus he has been studying: It is 685 kB, with 48 out of 65 apparently > functional "genes", the remainder being pseudogenes. Since Alu > sequences are present on average 1/5000 bp, you should be able to > find around 135 Alu repeats in his sequence. Hood is studying the > ways in which this contig. provides analytical information concerning > gene function; he is apparently trying to develop theories of how genes > might be demarcated using bioinformatic algorithims, rather than > exon trapping methods. > Lee is exagerating a bit--it's more like a factor of 3. There are six files in GenBank with human sequences greater than 100 kb. These are found in a table (pg 122) in the October 1995 issue of Nature Genetics in an excellent article by Richard Gibbs on the prospects for major human DNA sequencing (Gibbs 1995. Nat Genet 11:121-125.). Size (kb) Gene Acc # 685 T-cell receptor beta locus L36092 180 Retinoblastoma locus L11910 152 fmr1 locus L29074 152 Breakpoint-cluster region (BCR) U07000 130 IDS gene L43581 101 Neurofibromatosis type-1 locus L05367 There is some sequence from the Sanger Centre of chromosome 4p that may be long as well, but it is deposited as individual cosmids. These can also be found among those listed by Keith Robison (robison@nucleus.harvard.edu) on his web site "The 100 kb Club": http://golgi.harvard.edu/100kb/ Hope to see more soon, long range human sequence is still ~0.1% of the total (3.5 Mbp / 3 Gbp). From owner-chromosomes@net.bio.net Mon Oct 30 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!EMBL-EBI.ac.uk!tome From: tome@EMBL-EBI.ac.uk (Patricia Rodriguez-Tome) Subject: Re: Meaning of "AFM" in STS names Sender: news@ebi.ac.uk (Mr news) Message-ID: Date: Mon, 30 Oct 1995 15:03:04 GMT Distribution: inet Lines: 27 Reply-To: tome@ebi.ac.uk References: <4700dv$9db@metro.ucc.su.OZ.AU> Organization: European Bioinformatics Institute (EMBL) - UK X-Newsreader: mxrn 6.18-32 In article <4700dv$9db@metro.ucc.su.OZ.AU>, alcat@cyberspace.org (Alberto Catalano) writes: >Dear bionetters, > >Does nayone know the meaning and/or origin of the AFM nomenclature for >STS >sequences? >Thanks > > AFM = Association Francaise contre les Myopathies (French Association against muscular Distrophy). The STS made at Genethon (funded by AFM) in France begin with AFM For more information about Genethon : http://www.genethon.fr/genethon_en.html -- ======================================================================= Dr. Patricia Rodriguez-Tome | URL: http://www.ebi.ac.uk The EMBL Outstation, Hinxton - The European Bioinformatics Institute Hinxton Hall, Hinxton | Tel: +44 (0)1223 494 409 Cambridge CB10 1RQ, UK | Fax: +44 (0)1223 494 468 ======================================================================== From owner-chromosomes@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.tamu.edu!newshost.comco.com!news.texas.net!news.sprintlink.net!news.Edu.TW!news.cc.nctu.edu.tw!nctuccca.edu.tw!netnews.ntu.edu.tw!news From: b1205116@cc.ntu.edu.tw (Peng, Hsien-wei) Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.genome.chromosomes Subject: Re: FISH manuals etc. Date: 31 Oct 1995 06:58:58 GMT Organization: Inst. of Biomedical Science, Acad. Sinica, Taiwan Lines: 37 Message-ID: <474hfi$rkj@netnews.ntu.edu.tw> References: <46odrr$rg6@winx03.informatik.uni-wuerzburg.de> <4714c3$1tn@ixnews2.ix.netcom.com> NNTP-Posting-Host: @140.109.42.15 Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.6 Xref: biosci bionet.molbio.methds-reagnts:35658 bionet.cellbiol:3294 bionet.genome.chromosomes:895 >>- Are there lab-manuals with FISH protocols available? >> - Do you know good books about this technique? >>- Who organizes workshops and courses where he could learn this >>technique? >> - Have any reviews dealing with this topic been published recently? >>- Which are the landmark papers in this field? >> - Can you recommend suppliers of reagents/equipment? >>- etc................. >Have him contact the company Oncor. >They sell probes, kits, etc for FISH, and can give you all the >protocols. Don't happen to have their phone number handy, but I'm sure >you could look it up. >HK ========================================================================= Oncor: Phone 1-800-776-6267, 1-301-963-3500 FAX 1-301-926-6129 209 Perry Parkway, Gaitherberg, MD 20877, USA There is still another company selling a variety of pre-labeled probes -- Vysis: Phone 1-708-271-7000 FAX 1-708-271-7028 3100 Woodcreek Drive, Downes Grove, IL 60515, USA I have ever read a book talking about FISH protocols: -- In Situ Hybridization Protocols, K. H. Andy Choo ed. Humana Press, c1994 Or you can ask Boehringer Mannheim for their -- Nonradioactive In Situ Hybridization Application Manual Good luck ! From owner-chromosomes@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!news.uoregon.edu!news.dacom.co.kr!usenet.seri.re.kr!news.kreonet.re.kr!worak.kaist.ac.kr!usenet From: "Kernee H.Chang" Newsgroups: bionet.genome.chromosomes Subject: Q: GS system & BHK cells Date: 31 Oct 1995 01:23:59 GMT Organization: KAIST Lines: 24 Message-ID: <473trf$2sn@worak.kaist.ac.kr> NNTP-Posting-Host: kimjh.kaist.ac.kr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Dear Netters I’m trying to use Amplification vector system in mammalian cell line. Since I haven’t been using various system I’m wondering whether GS(glutamine synthetase) sysem fit to BHK host. I’ve never seen such a match but has anybody succeecced in the case ? Thanks a lot ! ______________________________________________ Kernee H. Chang Dept. Biological Sciences Korea Advanced Institute of Technology Taejon, Korea E-mail; kernee@bioneer.kast.ac.kr FAX: 042-869-2654 _______________________________________________ From owner-chromosomes@net.bio.net Tue Oct 31 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!uwm.edu!cs.utexas.edu!usc!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!ebi.ac.uk!EMBL-EBI.ac.uk!tome From: tome@EMBL-EBI.ac.uk (Patricia Rodriguez-Tome) Subject: Re: Meaning of "AFM" in STS names Sender: news@ebi.ac.uk (Mr news) Message-ID: Date: Tue, 31 Oct 1995 14:43:10 GMT Distribution: inet Lines: 44 Reply-To: tome@ebi.ac.uk References: <4700dv$9db@metro.ucc.su.oz.au> Organization: European Bioinformatics Institute (EMBL) - UK In article , dbarton@acer.gen.tcd.ie (Dr David E Barton) writes: >In article <4700dv$9db@metro.ucc.su.oz.au>, >Alberto Catalano wrote: >>Dear bionetters, >> >>Does nayone know the meaning and/or origin of the AFM nomenclature for >STS >>sequences? >>Thanks >> > >I've no idea of the meaning of all the heiroglyphics that follow AFM, >but >this prefix denotes markers developed by Genethon. > > | David Barton > | National Centre for Medical Genetics > | Our Lady's Hospital for Sick Children > | Crumlin, Dublin 12, Ireland. > | Tel +353 1 455 0515 Fax 455 8873 > Well, as I said before AFM=Association Francaise contre les Myopathies, the French muscular distrophy association that provides the funding for the research at Genethon and the hieroglyphics that follow AFM - come on, nobody recognized plate number and position on the plate ( well OK, it may not be true for all of them but you can see the pattern. If it is not the plate, it is the tube :-) Cheers Pat RT -- ======================================================================= Dr. Patricia Rodriguez-Tome | URL: http://www.ebi.ac.uk The EMBL Outstation, Hinxton - The European Bioinformatics Institute Hinxton Hall, Hinxton | Tel: +44 (0)1223 494 409 Cambridge CB10 1RQ, UK | Fax: +44 (0)1223 494 468 ======================================================================== From owner-chromosomes@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!HYPATIA.UNIVALLE.EDU.CO!jadkins From: jadkins@HYPATIA.UNIVALLE.EDU.CO (Adkins Andrew) Newsgroups: bionet.genome.chromosomes Subject: Re: FREE $$$ MAKING SOFTWARE !!! Date: 1 Nov 1995 13:45:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <475gnk$2t4@python.viper.net> NNTP-Posting-Host: net.bio.net Dear Sirs, I am interested in subscribing but must connect via Internet since I am in Colombia, South America. Can I do this? Please supply information. Further, Internet is relatively new to Colombia and I am interested in becoming a server. I would like to speak to someone about this, the potential for making REAL money exists since it is virgin teritory. Right now, only this university in Cali is connected and the public and professional people are dying for a connection. I am a US professional and am familiar with all the Net has to offer. There is much discussion about servers but so far, no one is doing it. I would like to get it set up early and take the first clients. If you have interest, please contact me. Thanks, Dr. Jerrold A. Adkins jadkins@hypatia.univalle.edu.co From owner-chromosomes@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!sgiblab!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.genome.chromosomes Subject: Help, What is longest contiguous sequence? Date: 1 Nov 1995 16:31:20 GMT Organization: UCSF, ITS Lines: 13 Message-ID: <4787co$p20@itssrv1.ucsf.edu> NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 31 Oct 1995 07:59:26 -0500 From: Chris Overton To: bgold@itsa.ucsf.edu Subject: Re: Help, What is longest contiguous sequence? See Bruce Roe's sequence on the BCR region of human chromosome 22 in GenBank--- it's in the neighborhood of 200KB of contiguous sequence (although may not be represented as such in GenBank). Also in collaboration with Bruce, we have another sequence in the DiGeorge region of chromosome 22 that is over 200KB and growing --- this has not yet been released. We have more than 1.5MB total sequence from 22 that should be coalescing in the next year. Chris Overton From owner-chromosomes@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!wizard.pn.com!news.zeitgeist.net!ico.net!pacbell.com!decwrl!lll-winken.llnl.gov!fnnews.fnal.gov!nntp-server.caltech.edu!ung From: ung@cco.caltech.edu (-Jin Kim) Newsgroups: bionet.genome.chromosomes Subject: Web page for Bacterial Artificial Chromosome libraries Date: 1 Nov 1995 23:03:07 GMT Organization: California Institute of Technology, Pasadena Lines: 9 Message-ID: <478ubb$q5m@gap.cco.caltech.edu> NNTP-Posting-Host: stucco.cco.caltech.edu Summary: Find out how to build/use BAC clones/libraries: www.tree.caltech.edu Keywords: BAC cloning technology now on WEB page:www.tree.caltech.edu X-Newsreader: NN version 6.5.0 #13 (NOV) We have recently put the information and protocols for the construction and use of Bacterial Artificial Chromosome clones and libraries. We hope this WEB page will help those people seeking to construct/use stable genomic clone resources. The address is: http://www.tree.caltech.edu Ung-Jin Kim