From owner-chromosomes@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!news00.sunet.se!sunic!news99.sunet.se!newsfeed.tip.net!peroni.ita.tip.net!venere.inet.it!usenet From: staff@ba.dada.it (EXE s.r.l.) Newsgroups: bionet.genome.chromosomes Subject: URGENT HELP FOR MIRIAM Date: Sun, 03 Dec 1995 11:33:09 GMT Organization: I.Net S.p.A. Lines: 25 Message-ID: <49s1r3$1kha@venere.inet.it> NNTP-Posting-Host: 194.185.15.150 X-Newsreader: Forte Free Agent 1.0.82 Hi I'm an Internet Provider of Barletta ( Bari ) Italy Sorry but i'm a newuser of NewsGroup and I don't Know exactly if i can post this article without subsribe it. It's for Miriam.... Miriam, twin, female, aged 10 months, is suffering from spinal (progressive) muscolar atrophy. Molecular test of DNA (carried aut in Rome at "A. Gemelli" Hospital, Medical School, Departement of Medical Genetics), shows: "Exon 7 of SMN gene (Motor Neuron Survival) is lost (by deletion). Coding sequence reveals, in one of the exons 7 of the cloned centromere, the insertion of a base in the intron 6.24 bp before exon 7" If you can give us useful informations about its treatment, call the following address: staff@ba.dada.it with subject: Miriam THANKS! From owner-chromosomes@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rjnai@aol.com (RJNAI) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Meeting announcement: DNA Forensics Date: 4 Dec 1995 15:26:02 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <49vlgq$o14@newsbf02.news.aol.com> References: <48vn3q$gru@xensei3.xensei.com> Reply-To: rjnai@aol.com (RJNAI) NNTP-Posting-Host: newsbf02.mail.aol.com Xref: biosci bionet.molbio.methds-reagnts:37163 bionet.genome.chromosomes:945 Hi, If you have any information about the deletion of the 9th chromosome please E-mail me.It is very important.Thank you veery much From owner-chromosomes@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rjnai@aol.com (RJNAI) Newsgroups: bionet.genome.chromosomes Subject: Deletion of the 9th Chromosome Date: 4 Dec 1995 15:29:39 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <49vlnj$o35@newsbf02.news.aol.com> Reply-To: rjnai@aol.com (RJNAI) NNTP-Posting-Host: newsbf02.mail.aol.com Hi, I'm looking for information on the deletion of the 9th chromosome.If anyone has information please E-mail me. From owner-chromosomes@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Conferenc on DNA Replication in Eukaryotes Date: 4 Dec 1995 23:23:27 GMT Organization: McGill University Lines: 65 Message-ID: <49vvtf$53i@sifon.cc.mcgill.ca> NNTP-Posting-Host: g-02.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) 2nd Announcement The Fourth McGill University Conference on Regulation of Eukaryotic DNA Replication St. Sauveur, Quebec, Canada October 1996. Organized by: Maria Zannis-Hadjopoulos and Gerald Price McGill Cancer Centre, Department of Oncology Faculty of Medicine and Faculty of Graduate Studies and Research McGill University http://www.mcgill.ca/mcgill/servers/Admin/UBO/dna.html The meeting will include plenary lecture sessions and poster sessions. TOPICS: * Eukaryotic Origins of DNA Replication * Nuclear and DNA Structure in DNA Replication * Modulation of Eukaryotic DNA Replication Preliminary Program The conference will be held at the Manoir Saint-Sauveur in the Laurentians. The first session will begin in the evening of October 17 and the final session will end in the early afternoon of October 20. Poster and oral presentations will take place on October 18 and 19. All participants are encouraged to present a poster on any of the topics that will be covered in the conference. Conference Location: The meeting will be held at the Laurentians' newest four-season conference resort hotel - a major luxury complex just about 45 minutes from Montreal, amid the dynamic beauty of the Laurentians. Saint-Sauveur-des-Monts, the Laurentians' most picturesque village, is the gateway village to the Laurentians. Transportation will be available from the airport to the conference site, and return. Registration materials and information regarding abstract submission will be sent in early 1996. *********Abstract deadline date: June 27, 1996.************ For More Information Please visit our Web Site http://www.mcgill.ca/mcgill/servers/Admin/UBO/dna.html From this site you can obtain: * A Preliminary Program * Further Information on Conference Location * A Reply Card to Obtain Registration Package G. Dellaire Exp. Medicine McGill University dellaire@odyssee.net From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!mn6.swip.net!seunet!news2.swip.net!mailgate.astra.com!usenet From: Lars Janzon Newsgroups: bionet.genome.chromosomes Subject: (no subject) Date: 6 Dec 1995 07:17:29 GMT Organization: Astra Arcus AB, Immunology R&D Lines: 13 Message-ID: <4a3g29$e7c@mailgate.astra.com> NNTP-Posting-Host: 157.96.68.13 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes?ALL Dear all, I would like to do FISH on CHO cell metaphase chromosomes. Does anyone know if there are chromosome specific probes for CHO cells or if there are other probes that can be used? Any help would be greatly appreciated. Lars Janzon Lars.Janzon@arcus.astra.se.com Astra Arcus AB Sweden From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!netnews.upenn.edu!cbil.humgen.upenn.edu!cbell From: cbell@cbil.humgen.upenn.edu (Callum Bell) Newsgroups: bionet.genome.chromosomes Subject: Inverse PCR on BACs/PACs Date: 5 Dec 1995 21:20:49 GMT Organization: University of Pennsylvania Lines: 8 Message-ID: <4a2d3h$fd3@netnews.upenn.edu> NNTP-Posting-Host: cbil.humgen.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.3] If anyone has successfuly used IPCR for the quick capture of ends of BAC and PAC clones please get in touch. If no replies, I'll experiment with different primers and post the sequences if there is any interest. Callum Bell From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!biosci!not-for-mail From: jboatri@emory.edu (Jeffrey H. Boatright) Newsgroups: bionet.cellbiol,bionet.cellbiol.cytonet,bionet.general,bionet.genome.chromosomes,bionet.journals.note Subject: New article in Molecular Vision Date: 5 Dec 1995 16:56:46 -0800 Organization: Emory University Lines: 26 Sender: kristoff@net.bio.net Distribution: world Message-ID: <4a2poe$ec3@net.bio.net> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.cellbiol:3599 bionet.cellbiol.cytonet:394 bionet.general:18762 bionet.genome.chromosomes:949 bionet.journals.note:543 The article ³A Polymorphic Trinucleotide Repeat at DXS8170 in the Critical Region of X-Linked Retinitis Pigmentosa Locus RP3 at Xp21.1² has been published in the Web journal _Molecular Vision_ at: http://www.emory.edu/MOLECULAR_VISION/index.html Molecular Vision is a peer-reviewed Web journal dedicated to the dissemination of research results in molecular and cell biology and genetics of the visual system (ocular and cortical).The journal accepts research articles, short reports, technical notes (*not necessarily vision-related*), and invited reviews. Manuscripts should be sent (via email or regular mail) to: Dr. Jeffrey H. Boatright Suite B5500 Emory Eye Center 1327 Clifton Road Atlanta, GA 30322 USA jboatri@emory.edu Thank you for your time, Jeff Boatright From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!nntp.coast.net!swidir.switch.ch!cisun2000.unil.ch!news From: Serge Leyvraz Newsgroups: bionet.genome.chromosomes Subject: PhD position Date: 6 Dec 1995 13:13:55 GMT Organization: CHUV (Lausanne) Lines: 20 Message-ID: <4a44uj$5vg@cisun2000.unil.ch> NNTP-Posting-Host: pc1bh10309.hospvd.ch Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) The research laboratory of The Centre Pluridisciplinaire d'Oncologie, University Hospital Lausanne Switzerland is recruiting a PHD or a Biologist with expertise in hematopoietic cell culture for the developpment of a project reagrding progenitor expansion, and tumor cell purging.The program consists in basic and clinically oriented research for the developpment of therapeutic approaches in solid tumor. the position is available in early 1996. Join our group and thanks for your help. Serge Leyvraz MD Chief Medical Oncology Tel : +41 21 314 01 50 Fax : +41 21 314 01 81 e-mail: Serge.Leyvraz@chuv.hospvd.ch From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!usc!news.cerf.net!newsserver.sdsc.edu!newshub.csu.net!charnel.ecst.csuchico.edu!csusac!csus.edu!news.ucdavis.edu!bullwinkle!ez041797 From: ez041797@bullwinkle.ucdavis.edu (Jean-Manuel Henry) Newsgroups: bionet.genome.chromosomes Subject: DNA sequences for plant pathogens Date: 6 Dec 1995 05:07:33 GMT Organization: University of California, Davis Lines: 14 Message-ID: <4a38el$g06@mark.ucdavis.edu> NNTP-Posting-Host: bullwinkle.ucdavis.edu Hi, I am looking for small ribosomal subunit rna sequences for some common plant/ seed pathogens. I am most interested in finding the rRNA sequence for "WAtermelon Fruit Blotch" (acidovorax avenae subsp. citrulli). I have checked all of the major resources on the web (small subunit databases and embl/ genbank) with no success. If anyone knows of any resources, texts, or persons that could help me locate some of these sequences please email me. Thank you, Jean-Manuel Henry UC Davis Department of Molecular and Cellular Biology From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Newsgroups: bionet.neuroscience,bionet.genome.chromosomes,bionet.cellbiol Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!utnut!utinfo!news From: bscott@spine.med.utoronto.ca (Brian Scott) Subject: visualization of newly divided neurons? X-Nntp-Posting-Host: spine.med.utoronto.ca Message-ID: Sender: news@utcc.utoronto.ca (News) Organization: Faculty of Medicine, University of Toronto Date: Wed, 6 Dec 1995 21:09:24 GMT Lines: 18 Xref: biosci bionet.neuroscience:11571 bionet.genome.chromosomes:955 bionet.cellbiol:3607 Hello, I'm trying to find out if there is a way of visually identifying living cells which have recently divided in order to do electrophysiological recording from them. Specifically, I'd like to label neural progenitor cells in the rat hippocampus postnatally and then wait until they've differentiated and taken up their normal functional role. Then I'd like to do slice recording from these young cells. I know of methods for identifying these cells in fixed tissue (e.g. autoradiography and bromodeoxyuridine immuno.) but is there a way of seeing these cells in living tissue? A fluorescent thymidine analogue which is taken up into dividing cells and could be viewed a number of weeks later would be lovely! :-) Brian From owner-chromosomes@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: genome data base Date: 6 Dec 1995 07:03:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512061502.KAA155756@r02n07.cac.psu.edu> NNTP-Posting-Host: net.bio.net Dear netters I need someone's help how can I connect to genome data base to find the sequence of microsatellite. Otherwise, does somebody know D11S988's sequence. If you help me, you will be very appreciated. Thanks Division of Experimental Pathology Department of Pathology, Penn State University College of Medicine, Hershey, PA 17033. U.S.A. Tel; 717-531-4710 From owner-chromosomes@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com (HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: genome data base Date: 7 Dec 1995 03:34:19 GMT Organization: Netcom Lines: 32 Message-ID: <4a5nbr$on2@cloner2.ix.netcom.com> References: <199512061502.KAA155756@r02n07.cac.psu.edu> NNTP-Posting-Host: ix-mia4-25.ix.netcom.com X-NETCOM-Date: Wed Dec 06 7:34:19 PM PST 1995 In <199512061502.KAA155756@r02n07.cac.psu.edu> cth3@EMAIL.PSU.EDU (Thomas Hwang) writes: > >Dear netters >I need someone's help how can I connect to genome data base >Penn State University College of Medicine, >Hershey, PA 17033. U.S.A. >Tel; 717-531-4710 > Web site for the genome data base is located at http://gdbwww.gdb.org/ or ftp at ftp.gdb.org or gopher at gopher.gdb.org IF you call them (410-955-9705) or fax them (410-614-0434) they will mail or fax you a form to fill out, after which they will provide you with a password, which will enable you to access more areas of the database. Helene University of Miami From owner-chromosomes@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!usc!news.cerf.net!news From: dcharles@chemscope.com Newsgroups: bionet.genome.chromosomes Subject: New Web Site:chemscope.com-Daily Biotechnology/Device News, FDA Approvals, Suppliers, Jobs, Web Pages Date: 7 Dec 1995 19:33:50 GMT Organization: CERFnet Lines: 20 Message-ID: <4a7fiu$4ic@news.cerf.net> NNTP-Posting-Host: default5.palto.cerfnet.com Visit chemscope.com, a daily web magazine dedicated to the biotechnology, medical device, and pharmaceutical industry. View: - Daily Industry News, Optional Daily Email/FAX Delivery - FDA Approvals, Warning Letters - Browse/Search Worldwide Suppliers Database (biologicals, lab instrumentation, medical materials) - Post or Read Device Equipment for Sale - Post or Read New Products - Post or Read Resumes (Public or Confidential) - Post or Read Company Job Listings - Personal/Company Web Pages @ $25/month - remote update using FTP, password protected - immediate forward responses to your Email or FAX machine - ChemScope hyperlinks - optional password restricted web access to pages David E. Charles, VP Marketing 415.568.1266 dcharles@chemscope.com From owner-chromosomes@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!usc!news.cerf.net!nic.cerf.net!morioka From: morioka@nic.cerf.net (Craig Morioka) Newsgroups: bionet.genome.chromosomes Subject: mtDNA Question Date: 7 Dec 1995 18:34:20 GMT Organization: CERFnet Dial n' CERF Customer Lines: 8 Message-ID: <4a7c3c$398@news.cerf.net> NNTP-Posting-Host: nic.cerf.net I'm looking for some info as to how human mitochondrial DNA is passed on from the mother to her progeny. Since mtDNA has its own circular genome 16.5 kbp the mitochondria must be passed on to the child through the mitochodria in the ovum. Is this correct? or is the mtDNA passed through the placentia? thanks for any help, From owner-chromosomes@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!EU.net!Germany.EU.net!zib-berlin.de!uni-duisburg.de!sun1.hrz.uni-essen.de!news From: Christian Schunck Newsgroups: bionet.genome.chromosomes Subject: Synthetic Chemical Endonucleases Date: 11 Dec 1995 14:16:08 GMT Organization: Universität GH Essen Lines: 25 Message-ID: <4ahef8$iun@sun1.uni-essen.de> NNTP-Posting-Host: t04b30.biologie.uni-essen.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Who knows how to get some special synthetic chemical nucleases as the following four: 2:1 1,10-phenanthroline -cuprous ion (OP-Cu) methidium propyl-EDTA-iron II (MPE) ferrous-EDTA tris (4,7-diphenyl-Tris(1,10-phenanthroline)ruthenium (II) Who has experiences with these substances? For further information about what substances I mean: Annu. Rev. Biochem., 1990, 59:207-36. I would be grateful about a short mail. -- ************************************************** C H R I S T I A N ****** S C H U N C K Universitaet GH Essen ** UE ** University of Essen FB 9 Genetik ****** Dept. of Genetics D-45117 Essen 45117 Essen Phone +49 201 183 2834 Mail christian.schunck@uni-essen.de Fax +49 201 183 2866 Germany ************************************************** From owner-chromosomes@net.bio.net Wed Dec 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: raveroni@aol.com (Raveroni) Newsgroups: bionet.genome.chromosomes Subject: Re: DNA help Date: 14 Dec 1995 15:21:10 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <4aq0vm$rki@newsbf02.news.aol.com> Reply-To: raveroni@aol.com (Raveroni) NNTP-Posting-Host: newsbf02.mail.aol.com I'm looking for a video by the name of "The Search For the Double Helix" starring Jeff Goldbloom. If anyone has any idea where I could get this, it would be greatly appreciated. thank you! From owner-chromosomes@net.bio.net Wed Dec 13 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!ns1.faseb.org!lamarck.sura.net!fconvx.ncifcrf.gov!tobin From: tobin@ncifcrf.gov (Greg Tobin) Subject: Mouse genetic markers Message-ID: Organization: Frederick Cancer Research and Development Center Date: Thu, 14 Dec 1995 22:31:14 GMT Lines: 10 I am looking for chromosome-specific markers suitable for making FISH probes for mouse gene localization. Rather than contact a different lab for each chromosome, I thought I'd ask if anyone has a lead on some mouse probes and possibly has several that I could use to get started. Thanks -Greg From owner-chromosomes@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!metro!angis.su.oz.au!kanemats From: kanemats@angis.su.oz.au (Kanematsu Institute) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes,bionet.cellbio Subject: Somatic cell hybrids using mouse A9 x human Date: 15 Dec 1995 04:19:28 GMT Organization: ANGIS, The University of Sydney, Australia Lines: 63 Distribution: inet Message-ID: <4aqt0g$fe1@metro.ucc.su.OZ.AU> Reply-To: kanemats@angis.su.oz.au (Kanematsu Institute) NNTP-Posting-Host: morgan.angis.su.oz.au Summary: advice on somatic cell hybrid Keywords: cell culture fusion hybrid human mouse chromosome HPRT HAT A9 leukemia Xref: biosci bionet.molbio.methds-reagnts:37659 bionet.genome.chromosomes:963 Dear bionetters, I am doing some experiments to optimise conditions for somatic cell hybridisation between human myeloid leukemia cells and mouse A9 cells (which are HPRT -ve fibroblasts and can be killed in HAT medium). My ultimate aim is to obtain a human x mouse hybrid that is: a) immortalized b) contains a derivative chromosome from the human leukemia cells isolated from its homologous human chromosomes (for gene mapping) I have been culturing the A9 cell in DMEM with 10% calf serum. In the course of culturing them, a small percentage of (sometimes multinucleated) giant cells appear. This occurs even when subcloned, so the giant cells are derived from the same population as the "normal" A9 cells. Does anyone know the cause of this? Is this normal? I have traced the history of the A9 line to carcinogenically transformed mouse fibroblasts (L cells) grown at the NCI in the 1940's. In an article in JNCI, it merely describes the appearance of the giant cells without giving any hypothesis regarding their origin. I would be interested in hearing from anyone with experience in culturing A9's to let me know of their experiences. I would also like to hear from anyone with experience in somatic cell fusion (particularly using A9 or human leukemia or normal white blood cells) using polyethylene glycol (PEG). The brand I'm using is BDH PEG 1500 at 35% w/v in serum free RPMI 1640, using the method described in: "METHODS IN MOLECULAR BIOLOGY VOL.29: Chromosome Analysis Protocols" Edited: John R Gosden, 1994, Humana Press Ch 17: 'Immortalized Cell Lines: Their creation and use in gene mapping' by Veronica van Heyningen. My specific questions are: 1) Will hybrids between (adherent) A9 and (non-adherent) human hematopoietic cells be adherent, non-adherent, semi-adherent or combinations of these? 2) How well do hybrids grow compared to the parent cells? 3) When and how quickly should I "wean" the hybrids of the HAT medium? 4) How efficient is the process of fusion when it works well? 5) What determines the rate of chromosome loss / retention for non-selected chromosomes? 6) How many human vs mouse chromosome does one expect in the hybrid clones? Answers to any of these questions (or advice on other related matters) would be much appreciated. Thanks Alberto Catalano e-mail: kanemats@morgan.angis.su.oz.au Ph: (02) 515-7453 Fax: (02) 515-6255 Kanematsu Laboratories * or * Kanematsu Laboratories Royal Prince Alfred Hospital Level 3, Blackburn Bldg, D06 Missenden Rd, Camperdown NSW 2050 Sydney University NSW 2006 Australia Australia From owner-chromosomes@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!warwick!griffin.nott.ac.uk!usenet From: Keith Bradnam Newsgroups: bionet.genome.chromosomes Subject: Re: DNA help Date: 18 Dec 1995 11:59:06 GMT Organization: Department of Genetics Lines: 7 Message-ID: <4b3l2a$21k@griffin.ccc.nottingham.ac.uk> References: <4aq0vm$rki@newsbf02.news.aol.com> NNTP-Posting-Host: evol.gene.nottingham.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; OSF1 V3.2 alpha) X-URL: news:4aq0vm$rki@newsbf02.news.aol.com raveroni@aol.com (Raveroni) wrote: >I'm looking for a video by the name of "The Search For the Double Helix" >starring Jeff Goldbloom. If anyone has any idea where I could get this, Wasn't this called something else? "Life Story" springs to mind, or maybe "The Story of Life". I think it was a BBC film wasn't it? Hope this helps. From owner-chromosomes@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!newsrelay.iastate.edu!news.iastate.edu!macgrant.agron.iastate.edu!user From: dgrant@iastate.edu (David Grant) Newsgroups: bionet.genome.chromosomes Subject: Re: DNA help Date: 19 Dec 1995 14:48:31 GMT Organization: Iowa State University Lines: 19 Message-ID: References: <4aq0vm$rki@newsbf02.news.aol.com> <4b3l2a$21k@griffin.ccc.nottingham.ac.uk> NNTP-Posting-Host: macgrant.agron.iastate.edu In article <4b3l2a$21k@griffin.ccc.nottingham.ac.uk>, Keith Bradnam wrote: > raveroni@aol.com (Raveroni) wrote: > >I'm looking for a video by the name of "The Search For the Double Helix" > >starring Jeff Goldbloom. If anyone has any idea where I could get this, > > Wasn't this called something else? "Life Story" springs to mind, or maybe "The > Story of Life". I think it was a BBC film wasn't it? Hope this helps. Raveroni is correct, the film was called "The Search for the DOuble Helix". I used to have a copy but eventually re-used the tape. David Grant dgrant@iastate.edu -- David Grant dgrant@iastate.edu 515-294-1205 From owner-chromosomes@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!HELIX.MGH.HARVARD.EDU!HAINES From: HAINES@HELIX.MGH.HARVARD.EDU ("Jonathan L. Haines") Newsgroups: bionet.genome.chromosomes Subject: Course on mapping human complex diseases Date: 19 Dec 1995 12:13:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 110 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HYZSGA93K28WW4NM@HELIX.MGH.HARVARD.EDU> NNTP-Posting-Host: net.bio.net ********************************* * COURSE ANNOUNCEMENT * ********************************* * GENETIC ANALYSIS METHODS * ********************************* * DEADLINE FOR APPLICATIONS * * JANUARY 15, 1996 * ********************************* GENETIC ANALYSIS METHODS FOR MEDICAL RESEARCHERS is a comprehensive four day course directed toward physician scientists and/or other medical researchers. The course will introduce state-of-the-art approaches for the mapping of human inherited disorders with an emphasis on the mapping of complex common diseases phenotypes. The overall focus of the course is on the development of a broad-based knowledge of the application of Human Genome Initiative resources to the design and execution of disease gene mapping projects. COURSE FACULTY: The course is taught by an internationally renowned faculty of scientists and physicians. Each faculty member has extensive expertise in several course topics. Co-organizers: Margaret A. Pericak-Vance, Ph.D., Department of Medicine, Department of Genetics, Duke University School of Medicine. She is a founding fellow of the American College of Medical Genetics and a board-certified Ph.D. medical geneticist with fifteen years experience in genetic counseling. Dr. Pericak-Vance is a genetic epidemiologist whose research has focused on the mapping of both simple mendelian and complex phenotypes of genetic disorders. She is actively mapping or has been involved in mapping such disorders as amyotrophic lateral sclerosis (Lou Gehrig disease), tuberous sclerosis, Alzheimer disease (AD), multiple sclerosis, primary open-angle glaucoma, and age-related macular degeneration. Jonathan L. Haines, Ph.D., Department of Neurology, Massachusetts General Hospital, and Harvard Medical School. Dr. Haines is a genetic epidemiologist whose research has focused on both human disease and general (reference) chromosome mapping. Dr. Haines has been involved in mapping such diseases as amyotrophic lateral sclerosis, Alzheimer disease, Huntington disease, tuberous sclerosis, neurofibromatosis, multiple sclerosis, primary open- angle glaucoma, and Batten disease. Instructors: Arthur S. Aylsworth, M.D., Division of Genetics and Metabolism, Department of Pediatrics, The University of North Carolina at Chapel Hill. Dr. Aylsworth is a board-certified pediatrician and clinical geneticist. As a physician- scientist, Dr. Aylsworth plays primary roles in family ascertainment and phenotypic delineation. He is actively involved in studies on neurofibromatosis and neural tube defects. David Goldgar, Ph.D., Genetic Epidemiology, Department of Medical Informatics, University of Utah. Dr. Goldgar is a statistical geneticist with extensive experience in both the theoretical and practical aspects of mapping human quantitative and complex traits. He has concentrated on the genetic epidemiology of common cancers, focusing on the genetic mapping of breast and ovarian cancer. Marcy MacDonald, Ph.D., Department of Neurology, Massachusetts General Hospital, and Harvard Medical School. Dr. MacDonald is a molecular geneticist with vast experience in applying molecular techniques to positional cloning problems. She has concentrated her efforts on the cloning and description of the Huntington disease gene and other trinucleotide repeat disease genes. Deborah A. Meyers, Ph.D., Department of Medicine, The Johns Hopkins University. Dr. Meyers is a statistical geneticist who has concentrated her research on the genetic basis of complex disorders including allergy, and psychiatric diseases such as schizophrenia and Alzheimer disease. She has extensive didactic experience in her teaching role at the short course in Medical and Experimental and Mammalian Genetics, The Jackson Laboratory, Bar Harbor, Maine. Marcy C. Speer, M.S., Ph.D., Department of Medicine, Duke University Medical School. Dr. Speer is a board-certified genetic counselor with twelve years of experience. She is also a genetic epidemiologist whose research interest is in mapping human genetic diseases, including the muscular dystrophies and neural tube defects. She is also involved in studies of unusual genetic phenomena such as anticipation and imprinting. Participation in the course will be dependent on completion of an application form that describes the applicant's background and research interests and is limited to 35 students. All participants will need to show evidence of a postgraduate genetics course or its equivalent. Participants must provide a brief statement describing their research interests, their reason for taking the course, and their long-term objectives in relation to the course curriculum. This information will be used to select a highly motivated participant group. Minority and women applicants are specifically encouraged to apply. A limited number of scholarships are available for registered students or fellows. Scholarship selection will be based on the strength of the individual applications. The course will be held at the Center for Executive Education on the campus of Babson College just outside of Boston, MA, U.S.A. It will be held from April 14th-April 17th, 1996. The total cost of the course is $1125. The DEADLINE for completed applications is JANUARY 15, 1995. For more information, brochures, and application forms please contact: Margaret A. Pericak-Vance, Ph.D. c/o Nadine Powers, Course Administrator Duke University Medical Center, Box 2900 Durham, N.C. 27710 (919) 684-6274 (voice); or (919) 684-6514 (fax) genclass@genemap.mc.duke.edu (email) or WWW:http://www.mc.duke.edu/depts/genetics/courses From owner-chromosomes@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!infoserv.rug.ac.be!lmb32!marcvdc From: marcvdc@lmb1.rug.ac.be Newsgroups: bionet.genome.chromosomes Subject: large intron in c.elegans Date: Tue, 19 Dec 1995 20:18:27 UNDEFINED Organization: Laboratory of Molecular Biology - University of Gent, Belgium Lines: 22 Message-ID: NNTP-Posting-Host: lmb32.rug.ac.be X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Dear Netters, First of all, I am not at all familiar with the c. elegans genome. However, I found protein homology between a mammalian cDNA and a c. elegans cosmid. Now I am wondering wether this is significant. There are already several open reading frames known in this c. elegans cosmid but they are quiet small. My cDNA has homology over 40.000 bp of the cosmid, implicating that I have putative introns of 5000 kb and 20.000 kb. Moreover, this putative c. elegans cDNA would overspan several characterised c. elegans cDNA's. Does anyone know wether this would be possible in c.elegans? Please also reply to my personal e-mail address. Marc Marcvdc@lmb1.rug.ac.be From owner-chromosomes@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!LAN.TJHSST.EDU!THORN From: THORN@LAN.TJHSST.EDU Newsgroups: bionet.genome.chromosomes Subject: Re: DNA help Date: 19 Dec 1995 07:49:10 -0800 Organization: TJHSST DOMAIN Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <37D0FFD64F0@lan.tjhsst.edu> NNTP-Posting-Host: net.bio.net > >I'm looking for a video by the name of "The Search For the Double Helix" > >starring Jeff Goldbloom. If anyone has any idea where I could get this, > > Wasn't this called something else? "Life Story" springs to mind, or maybe "The > Story of Life". I think it was a BBC film wasn't it? Hope this helps. This is available as a tape and as a laserdisk with backgound info about the protagonists AND the science. I believe Sunburst markets them. Check with a precollege teacher or get hold of the Sunburst or Videodiscovery catalogs. George Lucas produced the videodisk, so you may be able to get some info via the WWW if Amblin entertainment or Lucas have websites. I have seen the video and videodisk. Actually, there may even be a CD-ROM in the Sunburst set. I just don't have the catalogs around. Regards, Toby Mogollon Horn, Ph.D. *THE DNAHAND* Life Science and Biotechnology Laboratory Thomas Jefferson High School for Science and Technology 6560 Braddock Road Alexandria, VA 22312 phone 703-750-5024 fax 703-750-5010 From owner-chromosomes@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!EU.net!Austria.EU.net!newsfeed.ACO.net!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!dsi.unimi.it!master.cci.unibs.it!lunardi From: lunardi@master.cci.unibs.it (ITCPACLE LUNARDI - BRESCIA) Newsgroups: bionet.genome.chromosomes Subject: HELP: rare illness Date: Wed, 20 Dec 1995 10:30:13 Organization: Computer Science Dep. - Milan University Lines: 30 Message-ID: NNTP-Posting-Host: 192.167.18.170 X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Urgently Required Detailed information and the address of any hospitals or research institutes specialized in diseases of the blood especially anything relating to the diseases known as the MOSCHOWITZ SYNDROME OR THROMBOTIC MICRO ANGIOPATHY. Please send all information possible to any one of the following addresses: FAX:+39 30 6577098 TEL.: +39 30 657117 -657143 --------------------------------------------------------------------------------- MESSAGGIO URGENTISSIMO. Si richiedono dettagliate informazioni ed eventuali indirizzi di centri specializzati nello studio e nella cura di malattie del sangue: in particolare oggetto della richiesta è la malattia denominata PORPORA TROMBOTICA TROMBOCITOPENICA detta anche SINDROME DI MOSCHOWITZ, MICROANGIOPATICA TROMBOTICA. Le informazioni in nostro possesso sono: -malattia rara del bambino e dell’adulto, caratterizzata anatomicamente da una lesione diffusa delle arteriole e dei capillari che associa in spessimento dell’endotelio, deposito sotto-endoteliale di sostanze fibrinoidi e trombosi; clinicamente da una insorgenza acuta febbrile, una anemia emolitica intensa con emazie deformate, una porpora e a volte emorragie legate ad un abbassamento del tasso delle piastrine ematiche, lesioni renali con ematuria ed azotemia, manifestazioni neurologiche fugaci e variabili ed un evoluzione a poussèes e remissioni, ma sempre mortale. -rientra nel quadro delle anemie emolitiche microangiopatiche. DATA L’URGENZA Vi chiediamo inoltre di farci pervenire, se possibile, quanto richiesto ad uno dei seguenti recapiti: FAX: +39 30 6577098 TEL.: +39 30 657117 -657143 From owner-chromosomes@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!LAN.TJHSST.EDU!THORN From: THORN@LAN.TJHSST.EDU Newsgroups: bionet.genome.chromosomes Subject: Re: DNA help Date: 21 Dec 1995 17:57:13 -0800 Organization: TJHSST DOMAIN Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <3B738887AA3@lan.tjhsst.edu> NNTP-Posting-Host: net.bio.net The videodisk/CD-Rom etc on Life Story/the Race for the Double Helix---whatever its title...is available from WINGS for Learning/Sunburst 1600 Green Hills Rd. POBox 660002 Scotts Valley CA 95067-0002 800-321-7511 (as listed in the NSTA Education Suppliers 1995) Regards, Toby Mogollon Horn, Ph.D. *THE DNAHAND* Life Science and Biotechnology Laboratory Thomas Jefferson High School for Science and Technology 6560 Braddock Road Alexandria, VA 22312 phone 703-750-5024 fax 703-750-5010 From owner-chromosomes@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!agate!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!gatech!gt-news!prism!acmey!gt0619a From: gt0619a@acmey.gatech.edu (B) Newsgroups: bionet.genome.chromosomes Subject: Question on gen. eng. tech. Date: 16 Dec 1995 05:58:58 GMT Organization: Georgia Institute of Technology Lines: 17 Message-ID: <4atn72$b98@catapult.gatech.edu> NNTP-Posting-Host: acmey-prism.gatech.edu X-Newsreader: TIN [version 1.2 PL2] I have a question about the state of development of genetic engineering technology. I am wondering how long it will be before this technology is developed to the point that people will be able to go to the doctor and have their nearsightedness cured or other inherited disorders eliminated. Also, how soon will will it be before people will go to a geneticist MD to have the equivalent to cosmetic surgery done on themselves. Is this even in the foreseeable future of the technology? Any comments & responses would be appreciated. Thanks, Brian gt0619a@prism.gatech.edu From owner-chromosomes@net.bio.net Sun Dec 24 22:00:00 1995 Path: biosci!agate!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!news.sprintlink.net!gate.net!news-adm From: imxtc4u@gate.net Newsgroups: bionet.genome.chromosomes Subject: Genetics Question Date: 24 Dec 1995 23:16:45 GMT Organization: CyberGate, Inc. Lines: 20 Message-ID: <4bkn0t$ebg@news.gate.net> NNTP-Posting-Host: jaxfl2-8.gate.net X-Newsreader: SPRY News 3.03 (SPRY, Inc.) I not a regular reader of this group, so if the following question is not appropriate, my apologies. I have a couple questions that perhaps someone could answer for me. It has to do with genetics. If a person is a carrier of a defective gene for a neuro-muscular problem, I assume they wouldn't necessarily have any symptoms. Am I correct? Second if a father and mother were both carriers, would all the children suffer from the problem or would some of them be exepted for some reason or another? I am trying to determine where something started within the family. It has started with my father but none of his siblings (all females living) had the problem. Both my sister and I have neuromuscular problems of undetermined cause at this time. I've sent out letter to others with the same last name across the US to see if the same thing has happened within other branches of the family. So far, it has not. Kindly respond directly. From owner-chromosomes@net.bio.net Tue Dec 26 22:00:00 1995 Path: biosci!biosci!not-for-mail From: lstein@genome.wi.mit.edu (Lincoln Stein) Newsgroups: bionet.genome.chromosomes Subject: RELEASE 9 OF WHITEHEAD INSTITUTE PHYSICAL MAP OF HUMAN GENOME Date: 26 Dec 1995 17:59:14 -0800 Organization: Whitehead Institute for Biomedical Research Lines: 100 Sender: daemon@net.bio.net Distribution: world Message-ID: <4bq2cn$j1f@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: net.bio.net ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH HUMAN GENOMIC MAPPING PROJECT DATA RELEASE 9 (DECEMBER 1995) The ninth release of data from the Human Physical Mapping Project at the Whitehead Institute/MIT Genome Center, covering data generated through the end of December, 1995, is now available. This data release contains YAC screening data for 15,086 sequence tagged sites (STSs) screened on the CEPH mega-YAC library. For each STS, we report addresses for the YACs found to contain the STS. From the data obtained so far, there are 335 contigs assembled using single linkage between STSs. In addition, we also report a radiation hybrid map of the genome containing 6193 STS markers mapped on the Genebridge Panel, as well as integrations of the genetic, radiation hybrid and YAC contig maps. The data is available electronically in two ways. ANONYMOUS FTP: The entire data release is available as a set of Microsoft Excel files and tab-delimited ascii files on our ftp server. Using an ftp client (such as "Fetch" on the Macintosh), connect to ftp-genome.wi.mit.edu Use "anonymous" as your user name, and give your e-mail address as your password. The data files are present in the directory /distribution/human_STS_releases/sep95. The contents are as follows: 12-95.INTRO.txt Introduction to the data release, in straight text format 12-95.INTRO.html The same in HTML (World Wide Web) format 12-95.STS2YAC.txt STS & YAC screening data as tab-delimited text. chromosomes/ The same, split into smaller chromosome-specific files 12-95.YAC2STS.txt Inverse map of YAC to STS screening data 12-95.CONTIG2STS.txt YAC contig lists. 12-95.CONTIG2STS.txt The same, inverted. 12-95.sequence.txt Full sequences of STSs developed in-house. pictures/ Pictures of integrated maps in Macintosh and PS forms. rhmap/ Radiation hybrid maps. The data is also available in compressed form using the gzip program. THE WORLD-WIDE WEB: You will need a World Wide Web client such as Mosaic (Unix, MS-Windows and Macintosh) or MacWeb (Macintosh). Instruct your client to connect to http://www-genome.wi.mit.edu/ >From there, follow the "Human Physical Mapping Project" link. You will be able to browse and download the raw data set, view the individual and integrated maps, and to get information on the radiation hybrid and contig analyses. All mapped STSs are available through the Genome Database (GDB) and through GenBank. QUESTIONS AND PROBLEMS. If users have any questions or problems, please contact us at human_STS_help@genome.wi.mit.edu We invite suggestions about how to make these data release most useful. DATA RELEASE POLICY AND CITATION. Data releases are scheduled monthly. At the end of each month, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within a week of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy aims to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. The information for the human genome mapping project should be cited as: Whitehead Institute/MIT Center for Genome Research, Human Physical Mapping Project, Data Release 9 (December 1995). -- Lincoln D. Stein Whitehead Institute/MIT Genome Center lstein@genome.wi.mit.edu Cambridge, MA 02142 http://www-genome.wi.mit.edu/~lstein From owner-chromosomes@net.bio.net Tue Dec 26 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 27 Dec 1995 02:00:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512271000.CAA09309@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Wed Dec 27 22:00:00 1995 Newsgroups: bionet.genome.chromosomes Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!torn!blaze.trentu.ca!cegsm From: "Gary S. McKenzie" Subject: SRY and sex determination in drosophilia X-Nntp-Posting-Host: blaze.trentu.ca Content-Type: TEXT/PLAIN; charset=US-ASCII Message-ID: Sender: news@blaze.trentu.ca (USENET News System) Organization: Trent University Mime-Version: 1.0 Date: Thu, 28 Dec 1995 16:51:27 GMT Lines: 7 Hi I am interested in finding a review paper (within the last 2 mths) on sex determination and SRY in drosphilia complex. Additionally any groups that may be working currently on this area. Any information would be greatly appreciated. Thanx G.McKenzie cegsm@TrentU.Ca From owner-chromosomes@net.bio.net Thu Dec 28 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.nic.surfnet.nl!highway.leidenuniv.nl!jasper From: jasper%ruly46.leidenuniv.nl (Jasper Saris) Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics Question Date: 29 Dec 1995 12:55:55 GMT Organization: Leiden University, The Netherlands Lines: 36 Message-ID: <4c0ogr$jnh@highway.leidenuniv.nl> References: <4bkn0t$ebg@news.gate.net> NNTP-Posting-Host: ruly46.medfac.leidenuniv.nl X-Newsreader: TIN [version 1.2 PL2] imxtc4u@gate.net wrote: : I not a regular reader of this group, so if the following question is : not appropriate, my apologies. : I have a couple questions that perhaps someone could : answer for me. It has to do with genetics. If a person : is a carrier of a defective gene for a neuro-muscular : problem, I assume they wouldn't necessarily have any : symptoms. Am I correct? Correct, depending on several factors. If the variant gene has a recessive impact you need two not-normal funtioning copies. One on each chromosome. If the "defect" is dominant only one copie is sufficient for disease development. Often it is more complex and more genes are involved (genetic bakground variation) or environmental factors have a role. If you have at a point do get a name for your disease you could go to the OMIM database from GDB (Genetic DataBase?) USA, f.i. via www.gdb.org. There you can get more information. For instance on the pattern of inheritance. A genetic Counsellor should be able to "translate" the scientific talk if that would be a problem. Anyway he can explicate one the day-to-day long-term effects. Second if a father and mother : were both carriers, would all the children suffer from : the problem or would some of them be exepted for some : reason or another? Yes, depending of the disease type and particular inheritance of their chromosomes. : I am trying to determine where something started within : the family. It has started with my father but none of : his siblings (all females living) had the problem. Both : my sister and I have neuromuscular problems of undetermined : cause at this time. I've sent out letter to others with : the same last name across the US to see if the same thing has : happened within other branches of the family. So far, : it has not. Kindly respond directly. From owner-chromosomes@net.bio.net Thu Dec 28 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!istar.net!news1.ottawa.istar.net!fonorola!news.ottawa.istar.net!Rezonet.net!news.infobahnos.com!usenet From: "Stephane F. Kallos" Newsgroups: bionet.genome.chromosomes Subject: Re: Genetics Question Date: 28 Dec 1995 18:05:43 GMT Organization: Infobahn Online Services Lines: 39 Message-ID: <4bum9n$abp@news.infobahnos.com> References: <4bkn0t$ebg@news.gate.net> NNTP-Posting-Host: dialup6.infobahnos.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: imxtc4u@gate.net X-URL: news:4bkn0t$ebg@news.gate.net imxtc4u@gate.net wrote: >I have a couple questions that perhaps someone could >answer for me. It has to do with genetics. If a person >is a carrier of a defective gene for a neuro-muscular >problem, I assume they wouldn't necessarily have any >symptoms. Am I correct? Yes, assuming it is a recessive allele of the gene, which is what you are implying by the term "carrier." >Second if a father and mother >were both carriers, would all the children suffer from >the problem or would some of them be exepted for some >reason or another? Some of the children would suffer from the problem, some would be carriers, and others would be exempted. To calculate the probability (not absolute certainty), you would need to know if the gene is sex-linked. Your indication that no women that you know of suffer from the problem, is an indication that it may be sex-linked. Most of this information required can be determined (with an educated guess) by creating a large family tree which indicates the sex of the individual and whether or not they posses the trait. Don't forget that this becomes much more complicated with polygenic inheritance (when multiple genes affect a single trait). > >I am trying to determine where something started within >the family. It has started with my father but none of >his siblings (all females living) had the problem. Both >my sister and I have neuromuscular problems of undetermined >cause at this time. I've sent out letter to others with >the same last name across the US to see if the same thing has >happened within other branches of the family. So far, >it has not. Kindly respond directly.