From owner-chromosomes@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!morgan.angis.su.oz.au‚!kanemats From: kanemats@morgan.angis.su.oz.au‚ Newsgroups: bionet.genome.chromosomes Subject: Somatic cell hybrids using mouse A9 x human Date: 1 Jan 1996 20:56:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601020454.PAA25527@morgan.angis.su.OZ.AU> NNTP-Posting-Host: net.bio.net Summary: advice on somatic cell hybrid Keywords: cell culture fusion hybrid human mouse chromosome HPRT HAT A9 leukemia Dear bionetters, I am doing some experiments to optimise conditions for somatic cell hybridisation between human myeloid leukemia cells and mouse A9 cells (which are HPRT -ve fibroblasts and can be killed in HAT medium). My ultimate aim is to obtain a human x mouse hybrid that is: a) immortalized b) contains a derivative chromosome from the human leukemia cells isolated from its homologous human chromosomes (for gene mapping) I have been culturing the A9 cell in DMEM with 10% calf serum. In the course of culturing them, a small percentage of (sometimes multinucleated) giant cells appear. This occurs even when subcloned, so the giant cells are derived from the same population as the "normal" A9 cells. Does anyone know the cause of this? Is this normal? I have traced the history of the A9 line to carcinogenically transformed mouse fibroblasts (L cells) grown at the NCI in the 1940's. In an article in JNCI, it merely describes the appearance of the giant cells without giving any hypothesis regarding their origin. I would be interested in hearing from anyone with experience in culturing A9's to let me know of their experiences. I would also like to hear from anyone with experience in somatic cell fusion (particularly using A9 or human leukemia or normal white blood cells) using polyethylene glycol (PEG). The brand I'm using is BDH PEG 1500 at 35% w/v in serum free RPMI 1640, using the method described in: "METHODS IN MOLECULAR BIOLOGY VOL.29: Chromosome Analysis Protocols" Edited: John R Gosden, 1994, Humana Press Ch 17: 'Immortalized Cell Lines: Their creation and use in gene mapping' by Veronica van Heyningen. My specific questions are: 1) Will hybrids between (adherent) A9 and (non-adherent) human hematopoietic cells be adherent, non-adherent, semi-adherent or combinations of these? 2) How well do hybrids grow compared to the parent cells? 3) When and how quickly should I "wean" the hybrids of the HAT medium? 4) How efficient is the process of fusion when it works well? 5) What determines the rate of chromosome loss / retention for non-selected chromosomes? 6) How many human vs mouse chromosome does one expect in the hybrid clones? Answers to any of these questions (or advice on other related matters) would be much appreciated. Thanks Alberto Catalano e-mail: kanemats@morgan.angis.su.oz.au Ph: (02) 515-7453 Fax: (02) 515-6255 Kanematsu Laboratories * or * Kanematsu Laboratories Royal Prince Alfred Hospital Level 3, Blackburn Bldg, D06 Missenden Rd, Camperdown NSW 2050 Sydney University NSW 2006 Australia Australia From owner-chromosomes@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.u.washington.edu!root From: "Peter J. Myler" Newsgroups: bionet.genome.chromosomes Subject: cosmid sequencing Date: Wed, 03 Jan 1996 10:07:13 -0800 Organization: Seattle Biomedical Research Institute Lines: 35 Message-ID: <30EAC5D1.3970@u.washington.edu> NNTP-Posting-Host: cs20-14.u.washington.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (Win95; I) CC: mylerpj2u.washington.edu Hi folks: We are about to embark on a large scale cosmid (genome) sequencing venture and I would like to survey people to get their opionions on a couple of alternative approaches. We are planning to shotgun clone cosmid and sequence using an ABI 373A (and hopefully a 377 later). 1. subcloning - is it better to use M13 (ssDNA - better read lengths?) or a ds plasmid (e.g. pBluescript - better for joining contigs, 2 sequences per template). 2. screening subclones. Does it make sense (in terms of money & effort) to screen subclones (by restriction digestion and hybridization) to eliminate no-insert clones and clones containing cosmid vector DNA or just go ahead and sequence everyting and sort it out later? What about E.coli contamination? 3. sequencing reaction - dye primer vs dye terminator? When should you use TaqFS vs Thermosequenase? 4. what sort of read lengths are people routinely getting (from 373A, with & without stretch and 377)? Thanks to everyone in anticipation of your response. Regards Peter -- =======================================================================Peter J. Myler phone: (206) 284-8846x332 Seattle Biomedical Research Institute FAX: (206) 284-0313 4 Nickerson Street e-mail: MYLERPJ@U.WASHINGTON.EDU Seattle, WA 98109-1651 ======================================================================= From owner-chromosomes@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!kumc.edu!beslab3 From: beslab3@kumc.edu (Besharse Lab3) Newsgroups: bionet.genome.chromosomes Subject: Mapping to mouse chromosomes Date: 3 Jan 1996 14:16:31 -0800 Organization: KU Medical Center Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601032218.AA04693@www.kumc.edu> NNTP-Posting-Host: net.bio.net What is the fastest and/or easiest way to map a cDNA sequence to a mouse chromosome? Are hybridoma southerns or DNA available from a company or from individual laboratories? Please respond to the net. Thanks, David Osterbur From owner-chromosomes@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.ner.bbnplanet.net!das-news2.harvard.edu!oitnews.harvard.edu!purdue!yuma!usenet From: "D.L. Knudson" Newsgroups: bionet.genome.chromosomes Subject: Re: cosmid sequencing Date: Thu, 04 Jan 1996 14:05:08 -0700 Organization: Colorado State University Lines: 49 Message-ID: <30EC4104.45E5@klab.agsci.colostate.edu> References: <30EAC5D1.3970@u.washington.edu> NNTP-Posting-Host: klab.agsci.colostate.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (X11; I; SunOS 5.5 sun4m) To: "Peter J. Myler" CC: dknudson@lamar.colostate.edu I too would appreciate hearing from you regarding the responses that you receive to your questions. Preter J. Myler wrote: > > Hi folks: > > We are about to embark on a large scale cosmid (genome) sequencing > venture and I would like to survey people to get their opionions on a > couple of alternative approaches. We are planning to shotgun clone > cosmid and sequence using an ABI 373A (and hopefully a 377 later). > > 1. subcloning - is it better to use M13 (ssDNA - better read lengths?) > or a ds plasmid (e.g. pBluescript - better for joining contigs, 2 > sequences per template). > > 2. screening subclones. Does it make sense (in terms of money & effort) > to screen subclones (by restriction digestion and hybridization) to > eliminate no-insert clones and clones containing cosmid vector DNA or > just go ahead and sequence everyting and sort it out later? What about > E.coli contamination? > > 3. sequencing reaction - dye primer vs dye terminator? When should you > use TaqFS vs Thermosequenase? > > 4. what sort of read lengths are people routinely getting (from 373A, > with & without stretch and 377)? > > Thanks to everyone in anticipation of your response. > > Regards > > Peter > > -- > =======================================================================Peter J. Myler phone: (206) 284-8846x332 > Seattle Biomedical Research Institute FAX: (206) 284-0313 > 4 Nickerson Street e-mail: MYLERPJ@U.WASHINGTON.EDU > Seattle, WA 98109-1651 > ======================================================================= -- =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= Dr. Dennis L. Knudson, Professor of Entomology and Microbiology Department of Entomology Telephone: 970 491-7255 College of Agricultural Sciences Fax: 970 491-0564 Colorado State University Internet:dknudson@lamar.colostate.edu Fort Collins, CO 80523 USA URL http://klab.agsci.colostate.edu/ =*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*=*= From owner-chromosomes@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Chris Baker <73614.3607@CompuServe.COM> Newsgroups: bionet.genome.chromosomes Subject: DNA Date: 4 Jan 1996 23:12:49 GMT Organization: Walton High Lines: 6 Message-ID: <4chmth$qqo$1@mhafc.production.compuserve.com> I was sondering about research of ribosomal RNA and its functions. What is the currently known purpose of it? Chris Baker -- Chris Baker From owner-chromosomes@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news00.sunet.se!sunic!nntp.coast.net!harbinger.cc.monash.edu.au!bunyip.cc.uq.oz.au!news From: James Holman Newsgroups: bionet.genome.chromosomes Subject: Chromosome Counts Date: 4 Jan 1996 23:38:59 GMT Organization: Department of Botany, The University of Queensland Lines: 22 Message-ID: <4choej$fcq@dingo.cc.uq.oz.au> NNTP-Posting-Host: ecology3.botany.uq.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Hi everyone, I currently trying to carry out chromosome counts on Senna (small shrub) but have encountered problems. I am doing root tip squashes. One problem is that the roots are very fibrous and thin thus causing it to be difficult to macerate. The method I have employed entails 1. Put root tip in colchicine for 3 hrs. 2. Put in acetic alcohol for 24 hrs to fix. 3. Add drop of actic acid and macerate. Subject to low heat (stew). 4. Add drop of aceto orcin and further macerate. Subject to low heat 5. Blot off aceto orcin and replace with actic acid. 6. Squash the root tip under the coverslip. This has presently yeilded no results. I am considering trying young leaf material. I would greatly appreciate any suggestions (ie ideas, new methods.) that may aid in my search for these chromosomes. Thanks for your help Cheers James Holman. From owner-chromosomes@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!rutgers!uwm.edu!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: orr_fv@ix.netcom.com(Frank Orr ) Newsgroups: bionet.genome.chromosomes Subject: 16th chromosome Date: 5 Jan 1996 02:13:58 GMT Organization: Netcom Lines: 12 Message-ID: <4ci1h6$d05@ixnews7.ix.netcom.com> NNTP-Posting-Host: atl-ga15-02.ix.netcom.com X-NETCOM-Date: Thu Jan 04 6:13:58 PM PST 1996 I am the parent of a six year old boy who has a deletion in the Q portion of the 16th chromosome. I believe the deletion is of the 21st band.. poss some of the 20th and 22nd as well, if I get any responses I can get more accurate. I understand that a Doctor in Australia is doing research on the 16th but I don't have any specifics. I am trying to find any information that I can, this particular occurance is very rare and I have not had much luck. (my wife suggested the internet) I would very much like to correspond with any parents or anyone that might have any information on the 16th. Thanks From owner-chromosomes@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!WATSON.WUSTL.EDU!rwilson From: rwilson@WATSON.WUSTL.EDU (Rick Wilson) Newsgroups: bionet.genome.chromosomes Subject: Course Announcement Date: 5 Jan 1996 05:28:58 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601051326.AA07158@watson.wustl.edu> NNTP-Posting-Host: net.bio.net COURSE ANNOUNCEMENT ADVANCED GENOME SEQUENCE ANALYSIS March 20 - April 2, 1996 Cold Spring Harbor Laboratory XXXXXXXXXXXXXXXXXXXXXXXXXXX Course Application Deadline: January 15, 1996 XXXXXXXXXXXXXXXXXXXXXXXXXXX Ellson Y. Chen, Perkin Elmer Corporation Richard Gibbs, Baylor College of Medicine W. Richard McCombie, Cold Spring Harbor Laboratory Richard K. Wilson, Washington University Recent advances in the automation of DNA sequencing have opened new possibilities for the analysis of complex genomes at the DNA sequence level. This two week course will provide intensive training in this rapidly evolving field. The course will emphasize techniques and strategies for using automated sequences to sequence large, contiguous genomic regions. Students will carry out all of the steps in the sequencing process from preparing cosmid DNA to computer analysis of the finished sequence. Topics will include subclone library generation, large-scale template purification, sequencing reactions, gel analysis on automated sequencers, sequence assembly, gap filling and conflict resolution, Students will work in groups to sequence a large region of DNA. In the last year's course a 45kb cosmid was sequenced (GenBank) accession #U23729). Through this process they will be trained in crucial project and data management techniques. A series of lecturers will discuss their applications of these techniques as well as alternate strategies for high speed automated DNA sequencing. Last year's speakers included: C.T. Caskey, A. Dusterhoft, T. Marr, D. Smith, R. Smith, F. W. Studier, B. Roe, R. Weiss, and J.D. Watson. Additional information available through the CSHL web site: http://www.cshl.org/meetings/96cagsa.htm From owner-chromosomes@net.bio.net Thu Jan 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.tamu.edu!news From: Marianne Oprisko Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome Counts Date: Fri, 05 Jan 1996 13:55:32 -0800 Organization: tamu Lines: 23 Message-ID: <30ED9E54.1859@acs.tamu.edu> References: <4choej$fcq@dingo.cc.uq.oz.au> NNTP-Posting-Host: ppp15-17.rns.tamu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4a (Win16; I) To: James Holman James Holman wrote: > > Hi everyone, > I currently trying to carry out chromosome counts on Senna(small shrub) > but have encountered problems. I am doing root tip squashes. One > problem is that the roots are very fibrous and thin thus causing it to be difficult to macerate. James, I did this for my dissertation (nearly finished) on warmseason grass fiberous roots. Those root tips are tricky. are you sure you are only squashing the meristematic region. A good rule of thumb is from the upper edge of the root cap and 2-3 mm toward the stem. Try not to macerate too much since the meristematic cells are only a small ring within this disk. Also a group working with cotton here at Texas A&M are using a combination of cellulase and pectinase. You might want to give them a call--that's Stelle's lab thru the Dept. of Soil&Crop Sci. -- Marianne Oprisko Work: MOprisko@tamu.edu Home: MJO1354@acs.tamu.edu Voice Mail/Fax (409) 690-0738 From owner-chromosomes@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!gatech!newsfeed.internetmci.com!tank.news.pipex.net!pipex!peer-news.britain.eu.net!lyra.csx.cam.ac.uk!news From: Jo Fay Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome Counts Date: 8 Jan 1996 13:34:56 GMT Organization: The Sanger Centre Lines: 23 Message-ID: <4cr6i0$qfr@lyra.csx.cam.ac.uk> References: <4choej$fcq@dingo.cc.uq.oz.au> NNTP-Posting-Host: pabay.sanger.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.3 sun4m) X-URL: news:4choej$fcq@dingo.cc.uq.oz.au James Holman wrote: >Hi everyone, >I currently trying to carry out chromosome counts on Senna (small shrub) >but have encountered problems. I am doing root tip squashes. One >problem is that the roots are very fibrous and thin thus causing it to be >difficult to macerate. I had to do root tip squashes for c-banding as a project student, and found that softening the cell wall by partly digesting the cellulose made a big difference. I'm afraid I don't have the reference any more, but it was from a paper studying the c-banding of chromosomes of allium species. Another method was to completely digest the cell wall but that involves centrifugation and so uses more equipment. Jo -- __________________________________________________ | | | | Hug a furry animal | jmf@sanger.ac.uk | | today | | |____________________________|_____________________| From owner-chromosomes@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!biosci!not-for-mail From: phi94fpi@hpboot1.rz.uni-leipzig.de (i.V. Ralf Hofestaedt) Newsgroups: de.sci.medizin,de.sci.biologie,bionet.cellbiol,bionet.diagnostics,bionet.genome.chromosomes,bionet.info-theory,bionet.metabolic-reg,bionet.molbio.embldatabank Subject: Conference on Bioinformatics call for papers Date: 8 Jan 1996 12:10:44 -0800 Organization: University of Leipzig, Germany Lines: 150 Sender: daemon@net.bio.net Distribution: world Message-ID: <4crri1$npg@server2.rz.uni-leipzig.de> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.cellbiol:3752 bionet.diagnostics:521 bionet.genome.chromosomes:990 bionet.info-theory:3852 bionet.metabolic-reg:649 bionet.molbio.embldatabank:590 The following is a call for papers for the German Conference on Computer Science and Biology If you need an earlier decision on your submitted paper, so you can make your plans, please let us know. We could provide an early decision, although the partition of accepted abstracts into oral presentation and posters will take place only after June 15. If you need early acceptance, please submit your abstract by April 20. FIRST ANNOUNCEMENT COMPUTER SCIENCE AND BIOLOGY German Conference on B I O I N F O R M A T I C S (GCB`96) Leipzig, September 30th - October 2nd, 1996 The German Conference on Bioinformatics is organized on behalf of FG 4.0.2 Informatik in den Biowissenschaften of the German Society of Computer Science (GI) in cooperation with the AG of Computereinsatz in den Biowissenschaften of the German Society of Chemical Technique and Biotechnology (DECHEMA) and the AG Mathematische Modelle in Biologie und Medizin of the German Society of Medical Informatics, Statistics and Epidemiology (GMDS). The conference will take place at the University of Leipzig, September 30th - October 2nd, 1996, and is intended to bring together scientists who are addressing problems in biosciences and medicine using advanced computational methods including data modeling, simulation, artificial intelligence, computer graphics (visualization), robotics, combinatorial and stochastic optimization. The conference is concerned with all aspects combining computer science and biosciences. Topics of particular interest include, but are not limited to: - Genome Analysis - Models of Gene Regulation - Formal Languages and DNA - Molecular Docking and Recognition - Molecular Modeling and Protein Design - Models of Pattern and Structure Formation - Models in Cell Biology - Models of Dynamic Biological Systems - Self-Organization and Complex Systems - Metabolic Engineering - Metabolic Pathways - High Performance Computing - Biological Database Technology - Visualization and Animation of Biological Processes - Artificial Intelligence and Complex Systems - Evolutionary Computing - DNA Computing - Biological Paradigms in Computer Science Proceedings Extended abstracts of all accepted presentations including posters and computerdemos will be published in the conference abstract book which will be distributed to all participants. It is planned that full length papers will be invited by the PC for publication by Springer-Verlag "Lecture Notes of Computer Science" after the conference. Submission Procedures The closing date for receiving papers (extended abstracts of 3 pages), posters and computer demos (abstracts of 1 page) is May 1, 1996. The conference offers the possibility for the presentation of tutorials (abstracts of 1 page). The decision on acceptance for presentation will be communicated by June 15, 1996. Authors are urged to specify the category to which they are submitting their paper. Submissions must be written in English and should include title, author's name, mailing address, telephone number, fax number, email address and a list of keywords. They should be sent to: PD Dr. R. Hofestaedt Tel. 0341 / 9716100 Prof. Dr. M. Loeffler Fax 0341 / 9716109 University Leipzig email: GCB96@imise.uni-leipzig.de Department of Medical Informatics, Statistics and Epidemiology Liebigstr. 27 04103 Leipzig Organizing Committee R. Hofestaedt (Leipzig & Koblenz, GI-FG, Germany) T. Lengauer (Bonn, GI-FG, Germany) M. Loeffler (Leipzig, GMDS, Germany) D. Schomburg (Braunschweig, DECHEMA, Germany) Program Comittee J. Collado-Vides (Mexico) M. Mewes (Germany) A. Danchin (France) J. Shavlik (USA) A. Dress (Germany) S. Suhai (Germany) P. Karp (USA) M. Vingron (Germany) H. Kubinyi (Germany) E. Wingender (Germany) H. Lim (USA) H. Zima (Austria) M. Mavrovouniotis (USA) Important Dates Deadline of Submission: May 1, 1996 Notification of Acceptance: June 15, 1996 Receipt of Camera Ready Manuscript August 1, 1996 Registration Fees Full participation 250 DM GI, GMDS, DECHEMA 200 DM Student 80 DM One day registration 90 DM Includes: a copy of the proceedings, tea/coffee at the conference and food and drink at the poster session. ___________________________________________________________________ Registration form (to send preferably by email): GCB`96 Universitaet Leipzig Institut fuer Medizinische Informatik Phone 0341 / 9716100 und Statistik Fax 0341 / 9716109 Liebigstr. 27 email: GCB96@imise.uni-leipzig.de 04103 Leipzig Name: ____________________________________________ Institution: ____________________________________________ Address: ____________________________________________ Email: ____________________________________________ Tel. /Fax: ____________________________________________ I would like to participate in GCB`96 ( ) yes ( ) no ORAL ( ) POSTER ( ) COMPUTERDEMO ( ) From owner-chromosomes@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!news.unb.ca!coranto.ucs.mun.ca!news.nstn.ca!thor.atcon.com!usenet From: tandrews@atcon.com (Todd Andrews) Newsgroups: bionet.genome.chromosomes Subject: HLBA27 genetic marker, more info? Date: Mon, 08 Jan 1996 21:46:04 GMT Organization: Seldom, but sometimes Lines: 11 Message-ID: <4crvvl$lol@thor.atcon.com> Reply-To: tandrews@atcon.com NNTP-Posting-Host: cht-d131.atcon.com X-Newsreader: Forte Free Agent 1.0.82 I have been told that I have the genetic marker HLBA27, which is supposed to mark me as susceptable to ryghters(sp?) syndrome. I will assume that this marker/syndrome combination is truthful, but I will also question the diagnosis. I am curious about further susceptabilities to other syndromes/problems. For anyone familiar with said marker, thanks for any information. Thank you, -T From owner-chromosomes@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!tank.news.pipex.net!pipex!peer-news.britain.eu.net!warwick!news.coventry.ac.uk!leofric!sb14 From: Sandy Newsgroups: bionet.genome.chromosomes Subject: colour of eyes ? Date: Tue, 9 Jan 1996 14:53:23 +0000 Organization: Coventry University Lines: 22 Message-ID: NNTP-Posting-Host: sb14@leofric.coventry.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: sb14@leofric I do hope someone here can help me to understand how the colour of the eyes is determined. ( or indicate where I can find some information about it) I remember from lecturers ( it was years ago!) what happens when both parents have black eyes or both blue eyes or one black and the other blue. And I think I still remember the case when the eyes are brown. But that doesn't explain the possibility of green or grey eyes for the children. What is the difference between the colour blue and green ? I am no expert on that kind of topic so please exuse me if my question is silly or not correct. Thank you /Sandy sb14@coventry.ac.uk From owner-chromosomes@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.cais.net!news.his.com!news From: Dietmar Tietz Newsgroups: bionet.biophysics,bionet.diagnostics,bionet.genome.chromosomes Subject: --> CALL FOR PAPERS <-- Date: 9 Jan 1996 21:03:43 GMT Organization: Heller Information Services, Inc. Lines: 35 Message-ID: <4cul7f$gt3@news2.his.com> NNTP-Posting-Host: djt.his.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; 68K) To: djt@his.com X-URL: newsrc://news.his.com/ Xref: biosci bionet.biophysics:1572 bionet.diagnostics:528 bionet.genome.chromosomes:995 Dear Researchers: I am writing to you as the Editor of a book on Nucleic Acid Electrophoresis which is to be issued by Springer Verlag (Berlin, Heidelberg, New York), one of the leading scientific publishers. This book is intended to be part of the Springer Lab Manual Series which takes a practical approach and addresses a wide spectrum of readers extending from the college student to the senior investigator. The objective of this series is to communicate hands-on experience and to provide practical hints including easy-to-follow methods. At a time when research papers have become so short that they can barely describe the methods in sufficient detail, this lab manual will be a good opportunity to transmit to the scientific community a broader spectrum of your unpublished observations and valuable experience. Please contact me if you are interested in contributing a chapter. Our aim is to cover all aspects of electrophoresis. At this time, we are particularly interested in applications related to DNA sequencing and capillary electrophoresis. Thank you for your interest. Looking forward to hearing from you I remain, Sincerely yours, Dietmar Tietz, Ph.D. Fax: USA-(301)-681-7002 Email: djt@his.com or TVJ@CU.NIH.GOV WWW: http://www.his.com/~djt/ From owner-chromosomes@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!serra.unipi.it!t500.vol.it!peroni.ita.tip.net!venere.inet.it!usenet From: Cicinelli Newsgroups: bionet.genome.chromosomes Subject: traslation chromosome 1 and chromosome 2 Date: Tue, 09 Jan 1996 21:04:03 +0100 Organization: Dr. Ettore CICINELLI Lines: 30 Message-ID: <30F2CA33.417F@ba.dada.it> NNTP-Posting-Host: 194.185.15.139 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4 (Win95; I) My daughter has the following problem, as specified by Geneticist Doctor of S. Giovanni Rotondo Hospital in Italy. QUOTE The child has performed chromosomal analysis that has underlined an anomaly charac- terized from a traslation apparently balanced between a chromosome 1 and a chromo some 2. The chromosomal analysis of the parents has resulted normal and for such I motivate the present anomaly in daughter is insurgent "ex novo" for an error during the gametogenesis of one of the parents.Usually the traslation balance, kind if you inherit from a parent, they don't involve problems of mental delay. The traslation "ex novo" they are accompanied to mental delay in the 5% of the stricken subjects, with an extremely varying and unpredictable gravity. In conclusion, also if the problems neurologist of Ilaria could be brought back to a genetic cause, the certainty of this hypothesis is not currently verifiable. UNQUOTE My question is this one: <> From owner-chromosomes@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!news.pe.net!news.corpcomm.net!newspeak.ultratech.net!worldlinx.com!news.worldlinx.com!news From: anon1@anon.penet.fi (anon1@anon.penet.fi) Newsgroups: bionet.genome.chromosomes Subject: PLEASE HELP....re. rate of DNA replication... Date: Thu, 11 Jan 1996 16:43:17 GMT Organization: WorldLinx Telecommunications Inc. Lines: 16 Message-ID: <30f53e15.786941@news.odyssey.on.ca> Reply-To: anon1@anon.penet.fi NNTP-Posting-Host: 206.47.131.103 X-Newsreader: Forte Agent .99c/32.142 for a biology paper, i need to solve the follwing question: WHY IS DNA REPLICATION IN PROKARYOTES NEARLY 100 TIMES FASTER THAN REPLICATION IN EUKARYOTES ? i have wondered if it is because of (a) life span of pro vs eu karyotes, (b) because of existence ofa nuclear membrane in eukaryotes, where one is not found in prokaryotes... if anyone can shed some light on this question, please reply by posting or send email to: k.venkiteswaran@odyssey.on.ca thanks in advance! From owner-chromosomes@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!uwvax!newssinet!news.nc.u-tokyo.ac.jp!tkyex1.phys.s.u-tokyo.ac.jp!news.tisn.ad.jp!nalserver.nal.go.jp!statky2.stanet!news.imnet.ad.jp!news.affrc!inlink_81!shignak From: shignak@abr.affrc.go.jp (Shigeki "LARGE" NAKAYAMA) Newsgroups: bionet.genome.chromosomes,bionet.plants Subject: A Plant Chromosome Mailing List Date: 8 Jan 1996 23:47:57 GMT Organization: Natl Inst Agrobiol Resour, Tsukuba, Japan Lines: 25 Message-ID: NNTP-Posting-Host: inlink_81.abr.affrc.go.jp Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp X-Newsreader: NewsWatcher-J 2.0b19J12 Xref: biosci bionet.genome.chromosomes:998 bionet.plants:9881 Dear Plant Chromosomers: A Plant Chromosome Mailing List will be created for plant chromosome scientists using an e-mail. A mailing list is a single e-mail address for a set of users. By using a mailing list, users can send a message to everyone on that list by sending it to a single e-mail address. Only English is available for this mailing list. Please join this mailing list. When you will join in and/or introduce member(s) to this mailing list, please mail to shignak@abr.affrc.go.jp I am looking forward to your reply. Thank you very much. Shigeki Nakayama !(^_^)!|(-_^)||(-_-)|m(_ _)m<( )>w( - )w|(-_-)||(^_-)|!(^_^)! Shigeki Nakayama NIAR, Tsukuba, Japan shignak@ss.abr.affrc.go.jp !(^_^)!|(^_-)||(-_-)|w( - )w<( )>m(_ _)m|(-_-)||(-_^)|!(^_^)! From owner-chromosomes@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!panix!news.eecs.umich.edu!pravda.aa.msen.com!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.uni-c.dk!news From: Nikolaj Blom Newsgroups: bionet.genome.chromosomes Subject: Genome sizes Date: 15 Jan 1996 10:39:20 GMT Organization: Center for Biological Sequence analysis Lines: 38 Message-ID: <4ddaso$p8j@news.uni-c.dk> NNTP-Posting-Host: glyco.cbs.dtu.dk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.2 IP22) To: nikob@cbs.dtu.dk X-URL: news:bionet.genome.chromosomes Hello there, I am currently trying to compile a list of genome sizes of different organisms. I haven't been able to locate the genomic sizes of the following organisms. Can anyone point me to a source\ or provide the figures? Rattus norvegicus (rat) Oryza sativa (rice) Oryctolagus cuniculus (rabbit) Plasmodium falciparum Schizosaccharomyces pombe Sus scrofa (pig) Thanks in advance, Nikolaj Blom -- //\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\ / \ \ Nikolaj Blom, PhD-student / / Center for Biological Sequence Analysis \ \ phone1: +45 4525 2484 Department of Physical Chemistry / / phone2: +45 4525 2477 The Technical University of Denmark \ \ fax: +45 4593 4808 Building 206 / / e-mail: nikob@cbs.dtu.dk DK-2800 Lyngby Denmark \ \ / / WWW: http://www.cbs.dtu.dk/nikob.html \ \ / \\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\//\\// From owner-chromosomes@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!HELIX.MGH.HARVARD.EDU!HAINES From: HAINES@HELIX.MGH.HARVARD.EDU ("Jonathan L. Haines") Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 14 Jan 1996 18:29:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I00JULN4768WWAG5@HELIX.MGH.HARVARD.EDU> NNTP-Posting-Host: net.bio.net ********************************* * REVISED * * COURSE ANNOUNCEMENT * ********************************* * GENETIC ANALYSIS METHODS * ********************************* * DEADLINE FOR APPLICATIONS * * EXTENDED DUE TO BAD WEATHER * * JANUARY 19, 1996 * ********************************* Due to the unusually bad weather on the East Coast, and the resulting disruptions of mail and express mail delivery, the deadline for applying to this course has been extended until Friday, January 19th, 1996. Many applications have already arrived, and space is limited. To insure consideration of your application, please be sure that it arrives on or before the 19th. =========================================================================== GENETIC ANALYSIS METHODS FOR MEDICAL RESEARCHERS is a comprehensive four day course directed toward physician scientists and/or other medical researchers. The course will introduce state-of-the-art approaches for the mapping of human inherited disorders with an emphasis on the mapping of complex common diseases phenotypes. The overall focus of the course is on the development of a broad-based knowledge of the application of Human Genome Initiative resources to the design and execution of disease gene mapping projects. The course will be held at the Center for Executive Education on the campus of Babson College just outside of Boston, MA, U.S.A. It will be held from April 14th-April 17th, 1996. The total cost of the course is $1125. The DEADLINE for completed applications is JANUARY 19, 1995. For more information, brochures, and application forms please contact: Margaret A. Pericak-Vance, Ph.D. c/o Nadine Powers, Course Administrator Duke University Medical Center, Box 2900 Durham, N.C. 27710 (919) 684-6274 (voice); or (919) 684-6514 (fax) genclass@genemap.mc.duke.edu (email) or WWW:http://www.mc.duke.edu/depts/genetics/courses From owner-chromosomes@net.bio.net Mon Jan 15 22:00:00 1996 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.genome.chromosomes Subject: RE: DNA Replication Rates lower Eukaryotes and Prokaryotes... why a difference... Date: 15 Jan 1996 22:05:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 105 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601160531.AA01698@odyssee.net> NNTP-Posting-Host: net.bio.net >for a biology paper,=20 >i need to solve the follwing question: >WHY IS DNA REPLICATION IN PROKARYOTES NEARLY 100 TIMES FASTER THAN >REPLICATION IN EUKARYOTES ? The numbers I have quote: E.coli at 500 nt/s and Human Fibroblast at 50 nt/s that would be 10X faster >i have wondered if it is because of (a) life span of pro vs eu >karyotes, (b) because of existence ofa nuclear membrane in eukaryotes, >where one is not found in prokaryotes... >if anyone can shed some light on this question, please reply by >posting or send email to: k.venkiteswaran@odyssey.on.ca Okay=20 Here are a few possibilities I could come up with. One, in E.coli you are not worrying about mutations that much. In fact = a high mutation rate is desirable if the bacterium is to adapt to = changing environments. The result is you can replicate your DNA faster = without worrying about a mutation occurring. In higher eukaryotes such = as mammals this is a big problem... mutations can kill the whole = organism if well placed (i.e. cancer). For yeast I don't think this is = as strong a point as they are unicellular and much like in bacteria = --high mutation rates are adaptive. The error rate in E.coli is consequently ~10-8 where as in human = Fibroblasts it is ~10-10 Two in E.coli you have very little packing of DNA compared to higher = eukaryotes. To start DNA replication you need to gain access to the DNA = and expose the template for the polymerase. For instance you have the = HU protein of bacteria compared to H1, H2A, H2B, H3 and H4 as well as = the HMG proteins in mammals. The bacterial genome is much less complex = so by simple logic to replicate DNA it is much easier to gain accesss to = DNA in bacteria then in a mammal and hence it is faster in bacteria. =20 Further as a point of interest In E.Coli you only have one origin of replication where as in mammalian = cells it is estimated there are between 10 to the 4 --10 to the 6 = possible origins. This may reflects both the difference in size and = complexity of the two genomes as well as the speed at which DNA is = replicated. Multiple initiations of replication could help increase the = time to replicate the genome if it is very large. E.Coli has 3 X10 to the 6 bp and in the human genome there are ~3X 10 to = the 9 or=20 1000X more DNA to replicate. =20 1000 X 10 (the difference in speed of DNA replication)=3D 10 000 or 10 = to the 4 hmmmm by a rough calculation it makes sense why you have ~10 to the 4 = origins in the human genome and only 1 in bacteria. --------------------------------- Lastly, To your suggestion of the nuclear membrane as a possible reason for = higher replication rates I think the membrane provides a way of = segregating transcription and translation first of all and may not = really have much to do with replication on a surface level. =20 Graham Dellaire dellaire@odyssee.net From owner-chromosomes@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!EMAIL.PSU.EDU!cth3 From: cth3@EMAIL.PSU.EDU (Thomas Hwang) Newsgroups: bionet.genome.chromosomes Subject: PASTA Date: 17 Jan 1996 07:09:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601171507.KAA29260@r02n05.cac.psu.edu> NNTP-Posting-Host: net.bio.net Dear netters Does anyone know how can I search DNA sequence homology with PASTA? If you help me, you will be very appreciated. Thanks Department of Pathology, Penn State University College of Medicine Hershey, PA 17033,U.S.A. Tel;1-717-531-4710 Fax;1-717-531-5021 From owner-chromosomes@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: Re PASTA Date: 17 Jan 1996 17:15:42 -0000 Lines: 23 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4djaru$1fj@mserv1.dl.ac.uk> X-Mts: smtp X-Authentication-Warning: pluto.icr.ac.uk: Host localhost didn't use HELO protocol Original-To: biochrom@dl.ac.uk Dear Newsreaders , In response to Thomas Hwang's article : Dear netters Does anyone know how can I search DNA sequence homology with PASTA? If you help me, you will be very appreciated. Thanks Department of Pathology, Penn State University College of Medicine Hershey, PA 17033,U.S.A. Tel;1-717-531-4710 Fax;1-717-531-5021 I can only assume that the program he is looking for is FASTA in the GCG package . This is unless there is anyone out there working on the Spagetti or Macaroni genome project ( if there is please let me know ) . If FASTA is correct the help files with GCG should give you all the required information . David Cain From owner-chromosomes@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.genome.chromosomes Subject: CALL FOR DISCUSSION: AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) Followup-To: bionet.general Date: 18 Jan 1996 23:10:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 125 Sender: biohelp@net.bio.net Distribution: world Message-ID: <4dng4i$6t3@net.bio.net> NNTP-Posting-Host: net.bio.net We have received a proposal below for a new newsgroup, AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) Discussion on the following proposal will now be open through 28 January on BIOFORUM/bionet.general (*not* on BIONEWS/bionet.announce). For those who are not on the BIOFORUM/bionet.general newsgroup currently, either read bionet.general on USENET or contact one of the following biosci addresses to sign up by e-mail: Subscription Address Location -------------------- -------- biosci@daresbury.ac.uk Europe, Africa, and Central Asia biosci@net.bio.net Americas and the Pacific Rim One warning, however: BIOFORUM/bionet.general is a "chatty" newsgroup and many other items will be discussed there besides this newsgroup proposal. USENET news access is definitely better than e-mail if you want to monitor only this discussion. Discussion Guidelines --------------------- Please do not post one-line messages saying things like "I am in favor of such a newsgroup". We will collect votes later. The discussion period is an opportunity for anyone to bring up reservations about the proposed charter below and to propose modifications prior to the vote. If the charter is perfect as is, then there is no need for anyone to say anything!!! The newsgroup discussion leader may submit a revised proposal in light of the discussion, and the new charter would be included in the call for votes which will go out on 29 January (Newsgroup Discussion leaders - please note this deadline - if no revisions are received before the deadline, the original charter will be voted on.). Please be aware that only one vote is counted per e-mail address, so it is necessary to have your own account in order to vote. Multiple votes from the same address are not accepted. If you are interested in the following newsgroup, but do not have an e-mail address of your own, please obtain one from your computer center before the call for votes is issued. PLEASE NOTE that the topics covered under the charter below were previously included in the charter of the CHROMOSOMES/bionet.genome.chromosomes newsgroup. The latter group has not in practice focused on automated sequencing, so we have entertained this proposal to move an existing active mailing list into the BIOSCI/bionet hierarchy to focus specifically on this topic. --------------------------------------------------------------------- Proposal to create AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) USENET newsgroup name: bionet.genome.autosequencing Status: Moderated One line Description: Research and support on automated DNA sequencing Moderation address: bionet-genome-autosequencing@net.bio.net (autoseq-moderator@net.bio.net is an alias for bionet-genome-autosequencing@net.bio.net) Administrator: David Cain Mailing list name: AUTOMATED-SEQUENCING E-mail addresses: autoseq@net.bio.net autoseq@daresbury.ac.uk Newsgroup character: AUTOMATED-SEQUENCING/bionet.genome.autosequencing is a forum for the concentrated discussion and source of assistance on issues relating to automated DNA sequencing and fragment analysis such as microsatellite analysis and SSCP . AUTOMATED-SEQUENCING has been chosen as the name to represent the many different systems currently avaliable . Functions of the newsgroup: The newsgroup will offer a well needed focus for the discussion of automated DNA sequencing / fragment analysis which has until now been spread across a number of newsgroups resulting in many cross postings . The newsgroup will replace the one currently running on mailbase which has been running for just under a year and proaved to be well supported . Topics for discussion will include sequencing chemistries , template preparation methods , hints and tips for optimisation , recommended protocols for both large and small scale projects as well as providing a forum for support between scientists. Other aspects of the post-sequencing process will also be dealt with, for example, sequence troubleshooting and software issues. The newsgroup will also serve as a bulletin board for the announcement of meetings , conferences , job opportunities within the rapidly expanding sequencing market . Subscriptions are welcome from all academic institutions , hospitals , government agencies and other research facilities as well as commercial and industrial organisations . Contributions which support the outlined functions of the newsgroup will be actively encouraged . Moderation Policy: Chain letters , mass posted commercial messages and other messages deemed to be "Junk mail" will be deleted without comment . Inappropriate messages for commercial or legal reasons will be returned to the originator for editing and re-submission . Messages which are not strictly within the terms of the charter but still deemed to be of interest to the subscribers will be accepted as long as they do not breach any of the terms and conditions of the charter . Due to the commercial competitive nature of the subject companies with a vested interest will be encouraged to limit their activities on the newsgroup to replying directly to questions posted or clearing an article with the moderators prior posting to the list. Advertisements for commercial products are prohibited on the BIOSCI/bionet newsgroups. Proposed Moderators David Cain DNA sequencing Unit , Institute of Cancer Research , Chester Beatty Labs , London , UK Robert Feakes Department of Genetics , University of Cambridge , Cambridge , UK From owner-chromosomes@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!prodigy.com!usenet From: DJZF93A@prodigy.com (Ron Kirschbaum) Newsgroups: bionet.genome.chromosomes Subject: Trisomy 12 Date: 19 Jan 1996 05:56:14 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 10 Distribution: world Message-ID: <4dnbpu$17ig@usenetp1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 I recently had a miscarriage in which the pathology test came back as Trisomy 12. I have searched everywhere and cannot find anything on this subject. I've had 2 miscarriages and my husbands ex-wife has had one also. All 3 preg were lost within the first trimester. We were wondering if they can do tests to find out if this will continue to happen, and if so the names of the tests? There is no history of miscarriage(besides ourselves) or major birth defects on either side of the family. We are both 24 yrs old w/no living children. Thanks in advance. Jamie in Phoenix, AZ. From owner-chromosomes@net.bio.net Sat Jan 20 22:00:00 1996 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in2.uu.net!nih-csl!NewsWatcher!user From: fransh@Box-f.nih.gov (Frans Hochstenbach) Subject: Re: Genome sizes Message-ID: Sender: postman@alw.nih.gov (AMDS Postmaster) Nntp-Posting-Host: 156.40.148.31 Organization: National Institutes of Health References: <4ddaso$p8j@news.uni-c.dk> Date: Sat, 20 Jan 1996 23:12:06 GMT Lines: 17 Nikolaj Blom wrote: > I haven't been able to locate the genomic > sizes of the following organisms. Can anyone point me to a source\ > or provide the figures? ... > Schizosaccharomyces pombe The Schiz. pombe genome is estimated to be 13.4 Mb in size and is divided into three chromosomes, namely chromosome I (5.7 Mb), II (4.6 Mb), and III (3.5 Mb). For more info on Schiz. pombe, such as genomic sequencing, see http://www.nih.gov/sigs/yeast/fission.html Yours, ---Frans. From owner-chromosomes@net.bio.net Sun Jan 21 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!fdn.fr!jussieu.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!news.tau.ac.il!usenet From: anka Newsgroups: bionet.genome.chromosomes Subject: (no subject) Date: 22 Jan 1996 13:32:39 GMT Organization: Tel-Aviv University Computation Center Lines: 2 Message-ID: <4e03ln$26v@post.tau.ac.il> NNTP-Posting-Host: class9.tlv.ibm.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) sub From owner-chromosomes@net.bio.net Sun Jan 21 22:00:00 1996 Path: biosci!ODIN.MDACC.TMC.EDU!sa83506 From: sa83506@ODIN.MDACC.TMC.EDU Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 22 Jan 1996 14:40:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net list From owner-chromosomes@net.bio.net Mon Jan 22 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!EU.net!peer-news.britain.eu.net!uknet!ftel.co.uk!bham!bhamcs!news.ox.ac.uk!news From: Nada Matas Newsgroups: bionet.genome.chromosomes Subject: Chromosome 8 workshop Date: 23 Jan 1996 11:00:02 GMT Organization: Dept. of Pharmacology. Oxford University Lines: 3 Message-ID: <4e2f3i$d90@news.ox.ac.uk> NNTP-Posting-Host: somogyi.pharm.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes Is there a chromosome 8 workshop in the US this year, and if so, when? From owner-chromosomes@net.bio.net Mon Jan 22 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!EU.net!peer-news.britain.eu.net!uknet!ftel.co.uk!bham!bhamcs!news.ox.ac.uk!news From: Mike Stacey Newsgroups: bionet.genome.chromosomes Subject: chromosome 8 workshop Date: 23 Jan 1996 11:30:55 GMT Organization: Dept. of Pharmacology. Oxford University Lines: 4 Message-ID: <4e2gtf$e4i@news.ox.ac.uk> NNTP-Posting-Host: somogyi.pharm.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: mike.stacey@pharm.ox.ac.uk X-URL: news:bionet.genome.chromosomes Is there a Chromosome 8 workshop this year, and if so, when and where is it?? From owner-chromosomes@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!psinntp!psinntp!psinntp!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!news From: jesse@PO-Box.McGill.CA> Newsgroups: bionet.genome.chromosomes Subject: basic genetics Date: 24 Jan 1996 00:16:50 GMT Organization: McGill University Computing Centre Lines: 6 Message-ID: <4e3tpi$grv@sifon.cc.mcgill.ca> NNTP-Posting-Host: u-04.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) What evidence suggests that Down syndrome is more often the result of non disjunction during oogenesis rather spermatogenesis? ANY INFO WOULD BE GREATLY APPRECIATED! Thanks in advance! From owner-chromosomes@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!STJOHNS.EDU!YBRTBIO From: YBRTBIO@STJOHNS.EDU (rebekah rasooly) Newsgroups: bionet.genome.chromosomes Subject: (none) Date: 24 Jan 1996 06:58:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <24JAN96.10669355.0128.MUSIC@SJUMUSIC> NNTP-Posting-Host: net.bio.net one can examine down syndrome patients and determine which chromosomes 21 they have received using RFLP's. when this was done in a recent study (sherman et al., 1994, human molec. genetics 3:1529-35), it was determined that almost 90% were the result of maternal nondisjunction (311/352), of which 75% occured at meiosis I (that is, both maternal homologues were present in the trisomy 21 patient). rebekah rasooly, st. john's university From owner-chromosomes@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!JULIET.UCS.INDIANA.EDU!JCLAY From: JCLAY@JULIET.UCS.INDIANA.EDU (JANE CLAY) Newsgroups: bionet.genome.chromosomes Subject: Recombinant DNA courses at Indiana University Date: 24 Jan 1996 14:31:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601242229.RAA16743@cayman.ucs.indiana.edu> NNTP-Posting-Host: net.bio.net During the summer of 1996, Indiana University's Department of Biology, in cooperation with the I.U. Division of Continuing studies, will offer two week-long laboratory courses focusing on the techniques and procedures used in recombinant DNA research and their application. Participants also have the opportunity to work with a DNA sample of their own research organism, if they choose. Both courses will be taught on the Indiana University campus in Bloomington. The first course, "Recombinant DNA Technology," will introduce participants to procedures involved in recombinant DNA work and to the molecular aspects of genetic engineering. Most of the procedures that are taught to biology graduate students in the recombinant DNA section of a graduate techniques course at Indiana University will be covered. Participants can make arrangements to isolate genomic DNA from their own research organisms during the course. The following techniques will be included: *DNA and cloning vector manipulation *PCR technology *Preparation of recombinant DNA *Transformation of bacterial cells *Selection and assay of cloned and amplified fragments of "foreign" DNA *Transfer of DNA for probing (Southern blot) *Preparation of nonradioactive DNA probes The course is scheduled for June 3-7, 1996. Registration deadline is May 17. The second course, "Application of Recombinant DNA Technology: RFLP and Fingerprinting Analysis, RAPD Analysis, and DNA Sequencing," will provide participants with the opportunity to learn about the materials and techniques used in recombinant DNA research. Participants may bring a DNA sample to sequence during the course. This course will emphasize the following techniques: *DNA sequencing using non-radioactive methods *RAPD analysis of genomic DNA *Fingerprinting and RFLP analysis of genomic DNA *Electroporation of bacterial cells *Chemiluminescent detection of nucleic acids *Application of computers to DNA sequencing data analysis *Preparation of random fragment sequencing libraries and double-stranded DNA for sequencing *Use of bioneb cell and bipolymer disruption systems This course is scheduled for June 10-14, 1996. Registration deadline is May 24. For additional information, contact Jane Clay, Division of Continuing Studies, Owen Hall 204, Indiana University, Bloomington, IN 47405, phone (812) 855-6329. Internet: JClay@Indiana.edu Web: http://www.Indiana.edu/~scs/prof.html Jane Clay Associate Director Division of Continuing Studies Owen Hall 204 Indiana University Bloomington, IN 47405 Phone: 812-855-6329 Fax: 812-855-8997 email: JClay@Indiana.edu From owner-chromosomes@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!primus.ac.net!news.cais.net!nntp.uio.no!usenet From: rune.andreassen@rh.uio.no (Rune Andreassen) Newsgroups: bionet.genome.chromosomes Subject: VNTR D14S13 Date: 26 Jan 1996 20:27:33 GMT Organization: Universitet i Oslo Lines: 8 Message-ID: <4ebdfl$soj@ratatosk.uio.no> NNTP-Posting-Host: rhpc173.uio.no X-Newsreader: WinVN 0.92.6+ I would like to look at sequence variability within the repeat array in the VNTR D14S13 (CMM101). To do this I need to know some ofthe flanking sequence on both sides of the tandem array (to designe suitable PCR primers) Could anyone help me with information about, or how to get, the sequence of the flanking DNA in this VNTR? Thank you in advance, Rune. From owner-chromosomes@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!sunsite.doc.ic.ac.uk!charlie.lif.icnet.uk!callisto.lif.icnet.uk!schalkwy From: schalkwy@callisto.lif.icnet.uk (Leo Schalkwyk) Newsgroups: bionet.genome.chromosomes Subject: Re: Mapping to mouse chromosomes Date: 26 Jan 1996 21:20:38 GMT Organization: Imperial Cancer Research Fund Lines: 17 Distribution: world Message-ID: <4ebgj6$7l1@charlie.lif.icnet.uk> References: <9601032218.AA04693@www.kumc.edu> NNTP-Posting-Host: callisto.lif.icnet.uk X-Newsreader: TIN [version 1.2 PL2] There aren't the same hybrid resources as in the human, but there is a panel (not monochromosomal) of mouse/ hamster hybrids made by Y. Boyd and distributed by the HGMP resource center which can be used for chromo- some assignments. See http://www.hgmp.mrc.ac.uk/Public/Docs/hgmp-bio.html#Comparative mapping There is also a partial set of monochromosome hybrids prepared in P. Avner's lab. Besharse Lab3 (beslab3@kumc.edu) wrote: : What is the fastest and/or easiest way to map a cDNA sequence to a mouse : chromosome? Are hybridoma southerns or DNA available from a company or : from individual laboratories? Please respond to the net. Thanks, David : Osterbur From owner-chromosomes@net.bio.net Fri Jan 26 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 27 Jan 1996 02:00:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601271000.CAA03038@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Fri Jan 26 22:00:00 1996 Path: biosci!rutgers!uwm.edu!psuvax1!news.eecs.nwu.edu!newsfeed.acns.nwu.edu!news.acns.nwu.edu!news From: "Elena V. Zagariya" Newsgroups: bionet.genome.chromosomes Subject: Resume:research assistant Date: 26 Jan 1996 16:59:12 GMT Organization: Northwestern University, Evanston, IL, US Lines: 165 Message-ID: <4eb190$j9p@news.acns.nwu.edu> NNTP-Posting-Host: 165.124.232.44 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes C U R R I C U L U M V I T A E Name: Elena V. Zagariya E. mail: amz717@nwu.edu Visa Status: J-2 visa with US Job permit till 1997 Green Card - expecting in March 1996 Date/place of birth: April 6, 1969, Kiev, Ukraine Marital status: Married: Spouse - Alexander M. Zagariya, Ph.D., Daughter Olga (born Jan. 29, 1991) Residence: 374 Inland Drive, #2B, Wheeling, Illinois 60090, USA Phone/FAX: (708) 520-0948 Education and Positions: 1995 Pass Exams for attending Graduate School in Biology 1993 - 1994 Research Technician, Molecular Geriatrics Corp., 101 Waukegan Road, Suite #970, Lake Bluff, IL 60044, USA 1994 B. Sc. degree in Biology, Department of General and Molecular Genetics, Kiev University, (Kiev, Ukraine) GRE exam (Chicago, Illinois) 1993 TOEFFL exam (Birmingham, Alabama) 1990-1992 Research assistant and technician, Ukraine National Hospital #11, Ukraine Health Program, Kiev, Ukraine 1986-1990 B. Sc. degree in Economics, Kiev National Institute of Economy, (Kiev, Ukraine) 1986-1988 Kiev School of Nursing (Kiev, Ukraine) Certificate (Summa Cum Laude) Laboratory skills: -Mammalian Cell Culture, -Immunochemical staining, -Immunofluorescent microscopy, -Differentiation of Human Neuroblastoma Cells, -DNA isolation, -Gel electrophoresis, -Northern electroblotting, -Blood analysis (hematocrit), -pipettors, -autoclaves, -spectrophotometers, -solution and growth media preparation, -Basic molecular biology techniques Computer skills: -Macintosh, IBM AT(PC) and DOS operating systems -Windows 95 (and Windows 3.1 and MS-DOS 6.22) -All facets of the Internet (i.e. Gopher, Network, Usenet, E. mail) -Microsoft Word 5.1 and 6.0 -Drawing programs - Aldus superpaint and Cricket graph. Honors: 1990 - Kiev University young scientists competition, 2 prize 1991 - Kiev Hospital #11 young scientists competition Presentations and publications: 1. National Scientific Meeting of the American College of Rheumatology, October 21-26, 1995 at the Moscone Center and Marriott Hotel in San Francisco, California 2. E. Zagariya, G. Berdyshev. Frequency of human longevity in the Ukraine. Cytology and Genetics, V.23, N4, p.29-32, 1990 3. Participation in the International Symposium: Plant Biotechnology and Genetic Engineering, October 3-6, 1994, Kiev, Ukraine 4. S. Khrapunov, E. Zagariya, A. Dragan, A. Sivolob, and A. Zagariya Mechanisms of stabilizing nucleosome structure. Study of dissociation of histone octamer from DNA. In preparation. 1996 5. Differentiation of neurites growth during Alzheimer Disease. The American Society for Cell Biology, New Orleans, Louisiana, USA, December 11-15, 1993 6. Participation in the Symposia for Cancer Biology, May 14-17, 1990, Kiev, Ukraine References: 1. Karim Mehrazar, Ph.D., Assistant Professor, Clinical Coordinator, Indiana University, Northwest, Clinical Laboratory Science Program, 3400 Broadway, Gary, Indiana 46408-1197 Tel.: (219) 980-6923 FAX: (219) 980-6649 2. Leonid A. Sitailo, Ph.D., Research Associate, Department of Medicine, Loyola University, Chicago, Bldg. 54, Room 101, 2160 South First Avenue, Maywood, IL 60153, Tel.: (708) 343-7200, ext. 5915 FAX.: (708) 216-2792 3. Patrizia LoPresti, MD, Instructor, Department of Pediatrics, MC 4068, 5841 South Maryland Avenue, University of Chicago, WCHC - 486, Chicago, Illinois 60637 Tel.: (312) 702-6441 FAX: (312) 702-9234 4. Lester I. Binder, Ph.D., Professor, Department of Molecular, Structural and Cellular Biology, Northwestern University, School of Medicine, Evanston, IL 60208 Tel.: (312) 503-0823 FAX (312) 503-7912 5. Gennady D. Berdyshev, M.D., Ph.D., Professor, Chair, Department of General and Molecular Genetics, Kiev University, Dobrokhotova Street 4, Room 70, Kiev - 252142, Ukraine Tel.: 9011-7044-444-0761 6. Korneev Alexander, M.D., Ph.D., Senior Research Scientist, ERGO Science Corporation, 100 First Avenue, Charleston, MA 02129 Tel.: (617) 241-8900 FAX : (617) 241-8822 7. Tamara Diachkovskaya, Ph.D., Senior Research Associate, Kiev University, Dobrokhotova Street 4, Room 70, Kiev - 252142, Ukraine If I sound right for you, I am best reached by E. mail: amz717@nwu.edu From owner-chromosomes@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsxfer2.itd.umich.edu!gatech!taco.cc.ncsu.edu!newton.uncg.edu!hamlet.uncg.edu!clwisch From: "Carrie L. Wisch" Newsgroups: bionet.genome.chromosomes Subject: Hi! Date: Sun, 28 Jan 1996 22:13:50 -0500 Organization: The University of North Carolina at Greensboro Lines: 6 Message-ID: NNTP-Posting-Host: hamlet.uncg.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Hi! I just wanted to know what the outlook for jobs in biotechnology is. I am a freshman in college majoring in biology and concentrating in biotechnology and I just want to know. I haven't heard much news about the outlook. Thank you, Carrie From owner-chromosomes@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.genome.chromosomes Subject: CALL FOR VOTES: AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) Date: 29 Jan 1996 07:04:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 136 Sender: biohelp@net.bio.net Distribution: world Message-ID: <4einls$t7v@net.bio.net> Reply-To: biovote@net.bio.net NNTP-Posting-Host: net.bio.net Voting is now open on the following proposal to create the mailing list & newsgroup AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) The charter was not modified as a result of the discussion. *** NOTE *** We are often running several votes for other newsgroups, so please be certain to follow the voting directions *carefully*! If you just send in a message saying "YES" or "NO" it will not be counted if it is not clear which proposal you are responding to. ---------------------------------------------------------------------- Proposal to create AUTOMATED-SEQUENCING/bionet.genome.autosequencing (moderated) USENET newsgroup name: bionet.genome.autosequencing Status: Moderated One line Description: Research and support on automated DNA sequencing Moderation address: bionet-genome-autosequencing@net.bio.net (autoseq-moderator@net.bio.net is an alias for bionet-genome-autosequencing@net.bio.net) Administrator: David Cain Mailing list name: AUTOMATED-SEQUENCING E-mail addresses: autoseq@net.bio.net autoseq@daresbury.ac.uk Newsgroup character: AUTOMATED-SEQUENCING/bionet.genome.autosequencing is a forum for the concentrated discussion and source of assistance on issues relating to automated DNA sequencing and fragment analysis such as microsatellite analysis and SSCP . AUTOMATED-SEQUENCING has been chosen as the name to represent the many different systems currently avaliable . Functions of the newsgroup: The newsgroup will offer a well needed focus for the discussion of automated DNA sequencing / fragment analysis which has until now been spread across a number of newsgroups resulting in many cross postings . The newsgroup will replace the one currently running on mailbase which has been running for just under a year and proaved to be well supported . Topics for discussion will include sequencing chemistries , template preparation methods , hints and tips for optimisation , recommended protocols for both large and small scale projects as well as providing a forum for support between scientists. Other aspects of the post-sequencing process will also be dealt with, for example, sequence troubleshooting and software issues. The newsgroup will also serve as a bulletin board for the announcement of meetings , conferences , job opportunities within the rapidly expanding sequencing market . Subscriptions are welcome from all academic institutions , hospitals , government agencies and other research facilities as well as commercial and industrial organisations . Contributions which support the outlined functions of the newsgroup will be actively encouraged . Moderation Policy: Chain letters , mass posted commercial messages and other messages deemed to be "Junk mail" will be deleted without comment . Inappropriate messages for commercial or legal reasons will be returned to the originator for editing and re-submission . Messages which are not strictly within the terms of the charter but still deemed to be of interest to the subscribers will be accepted as long as they do not breach any of the terms and conditions of the charter . Due to the commercial competitive nature of the subject companies with a vested interest will be encouraged to limit their activities on the newsgroup to replying directly to questions posted or clearing an article with the moderators prior posting to the list. Advertisements for commercial products are prohibited on the BIOSCI/bionet newsgroups. Proposed Moderators David Cain DNA sequencing Unit , Institute of Cancer Research , Chester Beatty Labs , London , UK Robert Feakes Department of Genetics , University of Cambridge , Cambridge , UK ---------------------------------------------------------------------- Voting is now open on the proposal for AUTOMATED-SEQUENCING/bionet.genome.autosequencing and will run through 24:00 hrs Pacific Time on 27 February 1996. Please send your vote to either of the following addresses: Address Location Network ------- -------- ------- biovote@daresbury.ac.uk U.K. JANET biovote@net.bio.net U.S.A. Internet/BITNET PLEASE BE SURE TO FOLLOW THE FORMAT BELOW - WE OFTEN RUN MORE THAN ONE VOTE AT A TIME SO A SIMPLE "YES" OR "NO" MESSAGE WITHOUT THE NEWSGROUP NAME MAY BE AMBIGUOUS. Your vote should contain a single line: YES on AUTOSEQ if you favor allowing the creation of this newsgroup or NO on AUTOSEQ if you think that this proposal will adversely affect the BIOSCI/bionet system. While not intended to be an exhaustive list of possible concerns (more specific concerns may have been raised during the discussion period on BIOFORUM/bionet.general and interested readers are referred to these), some general reasons for voting NO might be if you are concerned about newsgroup proliferation and/or believe that the proposed group will not be utilized, or if you think that the proposed newsgroup would substantially duplicate or overlap with the function of existing newsgroups. If you are simply not interested in participating in the newsgroup above, please don't cast a NO vote, but instead just don't vote at all. The newsgroup proposal must receive at least 80 YES votes to pass and the number of YES votes must be greater than the number of NO votes by at least 40. Discussion of the newsgroup proposal is now closed and we strongly discourage posting any messages in other forums about the fact that a CALL FOR VOTES has been issued. Permission is granted to distribute this to the current AUTOSEQ mailing list and the BIOSCI CHROMOSOMES newsgroup. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Mon Jan 29 22:00:00 1996 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!gatech!newsfeed.internetmci.com!vixen.cso.uiuc.edu!uchinews!bmimac3.bsd.uchicago.edu!user From: edesjard@ben-may.bsd.uchicago.edu (Ed DesJardins) Subject: Genome Factoids Wanted X-Nntp-Posting-Host: bmimac3.bsd.uchicago.edu Message-ID: Followup-To: bionet.genome.chromosomes Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago Date: Tue, 30 Jan 1996 21:02:38 GMT Lines: 29 Does anyone know the (approximate) answers to the following questions? I was asked these questions and wasn't quite sure if I was in the right ball park. 1) What percentage of the human genome is protein coding information? 5%? Less than 5%? Do these estimates include or exclude introns in the calculation of "coding regions?" 2) What percentage of the human genome has been sequenced? 1%? A few percent? I assume that the vast majority of known human sequence comes from protein coding regions? 3) What percentage of the 100,000 or so genes that make up a human have been cloned (sequenced) at the cDNA level (excluding ESTs)? 5%? 10%? 4) Of the 100,000 or so expressed human genes, how many have been identified by EST? 50% More than 50%? Thanks. Ed edesjard@ben-may.bsd.uchicago.edu From owner-chromosomes@net.bio.net Tue Jan 30 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.nic.surfnet.nl!elsevier.nl!surfnet.nl!news.sara.nl!news From: renger@anal.chem.uva.nl (Renger Jellema) Newsgroups: bionet.genome.chromosomes Subject: DNA / genome Date: Wed, 31 Jan 1996 12:53:19 GMT Organization: Academic Computer Services Amsterdam (SARA) Lines: 21 Message-ID: <4enoot$o3c@mira.sara.nl> NNTP-Posting-Host: anal-pc12.chem.uva.nl X-Newsreader: Forte Free Agent 1.0.82 To All: I'm looking for a very basic picture of DNA. Actually it should only contain something like 2 threads wounded together as a double helix. Can anyone supply me with such a picture (BMP, JPG, GIF or something like that). Maybe there's a WEB site which contains such a picture. But I didn't find it (yet). THX, Ranger. --------------------------------------- \|||/ @ @ 100% Chemical and proud of it. * --------------------------------------- Renger@anal.chem.uva.nl http://analserv.chem.uva.nl From owner-chromosomes@net.bio.net Wed Jan 31 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!Germany.EU.net!wizard.pn.com!news.xensei.com!news From: symposia@cambridge.org (James Larkin) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes,misc.legal Subject: Meeting announcement: DNA Forensics Date: Thu, 01 Feb 1996 17:05:05 GMT Organization: Cambridge Symposia Lines: 59 Message-ID: <4eqrtt$dhv@xensei3.xensei.com> Reply-To: symposia@cambridge.org NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.molbio.methds-reagnts:39624 bionet.genome.chromosomes:1022 misc.legal:112708 Cambridge Symposia, organized to serve the scientific community through the administration of interdisciplinary conferences in the biological sciences, is announcing their upcoming meeting, "DNA Forensics: Science, Practice, and Future," to be held April 21-27, 1996 in Sante Fe, New Mexico. Topic Description: Almost twenty years back it was shown that the blue print of life, the deoxyribonucleic acid (DNA), varies enormously in its structure and organization between individuals, and the recombinant DNA technology allows one to unravel this variation. More tha n eleven years have passed since the world has witnessed that by typing DNA from biological samples left at crime scenes, it is possible to exonerate a wrongly accused suspect, and the same technique does indeed identify the true perpetrator. Since then, versions of the same technology have been used in numerous cases around the world to exculpate suspects and to convict criminals. In civil litigations of identifying parents and relatives as well, DNA typing has played a major role around the world. Fo rensic and medico-legal experts, in unison, agree that the DNA technology is the most powerful tool that a scientist can offer for forensic investigations, even more diversely usable than the traditional fingerprinting imaging. In spite of the tremendous success of using DNA in forensics around the world, such well-publicized criticisms by otherwise well known experts, raise a few questions. Why are there such contradictory views? Is the science of DNA typing still in its adolescence that needs more ma turity to apply in legal cases? Is the practice of DNA typing fallible to the extent that the results are not trustworthy? If DNA typing is so controversial in forensics, how reliable are its applications in medicine and biology? What is in store for t his technology for future? The theme of this conference is to discuss these basic issues. While a number of recent meetings and symposia have discussed these issues in platforms that varies from sophisticated technical details to educating general publi c, the goal of this meeting is to put the science, practice, and future of DNA forensics in their proper perspective. Experts encompassing molecular geneticists, forensic practitioners, legal authorities, and statistical geneticists, will be invited to initiate discussions on these subjects. The meeting will be divided in three parts consistent with the major themes. In each session the main presentations will be grouped in thematic format, at the conclusion of each of such sessions time for discussion will be preserved to raise alternative views and their justification. ---------------------------------------------------------------------------------------------------------- For immediate information on this meeting, including a full program draft, please see our website: http://www.cambridge.org/symposia/ James Larkin Cambridge Symposia 1037 Chestnut Street Newton Upper Falls, MA 02164 tel. (617) 630-1399 fax. (617) 630-1395 symposia@cambridge.org