From owner-chromosomes@net.bio.net Thu Feb 01 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: opv.lsp@ix.netcom.com(OPV Lifescience Partners ) Newsgroups: bionet.genome.chromosomes Subject: BRCA1 Genetic Lab Looking for Operations Director Date: 2 Feb 1996 19:22:14 GMT Organization: Netcom Lines: 126 Message-ID: <4eto96$cqb@ixnews3.ix.netcom.com> NNTP-Posting-Host: sfo-ca6-10.ix.netcom.com X-NETCOM-Date: Fri Feb 02 11:22:14 AM PST 1996 OPV Lifescience Partners email opv.lsp@ix.netcom.com fax 415 674-1965 OPV Lifescience Partners has been retained to identify and place a Director of Operations for a cutting edge genetic reference lab. Please read the position description and determine whether you are an appropriate candidate. If you are please email ( no attachments) a resume and cover letter to opv.lsp@ix.netcom.com or fax us at 415 674 1965. All resumes will be reviewed, please do not attempt to contact our client directly as we are the retained agent for this position search and your direct contact will not improve chances of placement. We appreciate your time and immediate application for this position. _______________________________________________________________________ ______ Position Description Director of Operations REPORTS TO: President, Myriad Genetic Laboratories, Inc. RESPONSIBILITIES: The Director of Operations will have responsibility for managing day-to-day operations of what will become a very high volume, state-of-the-art genetic testing laboratory. This person will be responsible for the developme nt, planning, carrying out and control of testing methods, processes and operations. The Director of Operations will be responsible for hiring, managing and motivating a supervisory team through a major scale up in capac ity and service volume. Experience planning, implementing and maintaining of clinical laboratory procedures, processes and operations at the highest professional standards; Outstanding people skills for hiring, directing and motivating key personnel. History of hands on management and getting things done. Experience with laboratory processes based on DNA testing. Specifically, this individual will be responsible for planning, organizing and managing the scale up and operation of a rapidly growing commercial clinical laboratory. The Director of Operations will be responsible for: Producing accurate test results in a turnaround time sensitive service business. Developing processes and technologies to improve service, reduce costs and assure continued compliance with CLIA and other regulatory. Assuring timely and effective responses to customer inquiries and complaints. Planning and coordinating the scale up of manpower, equipment, facilities, materials and controls to deliver superior service as volume expands. EDUCATION: Undergraduate degree in life science An advanced degree in molecular biology, genetics. CANDIDATE QUALIFICATIONS: A minimum of five years management and supervisory experience in a clinical reference laboratory. Excellent management and communication skills, both verbal and written. The ability to deal effectively with all levels of employees. A keen sense of differences in disciplines and the ability to affect team performance. A solid understanding of service goals, including accuracy, turnaround time and customer satisfaction. PROFILE: Highly energetic, results oriented and dedicated to achieving established and personally defined goals. Excellent interpersonal, motivational and communication skills. Strong leadership skills; exhibit a strong presence and ability to develop and command the respect of others. A depth of management experience and ability to take charge of the major undertaking involved in this position. Technical capabilities to understand and incorporate the technologies used in Myriad Labs' testing into the factors for success and economy. (Board certification for DNA testing a plus.) Sensitivity to the social and ethical issues that surround the right of privacy and the potential discriminatory ways disease predisposition testing might be used if inadequate controls existed. An ability to communicate well with both superiors and subordinates in developing and completing multiple projects in a result driven environment. LOCATION: Salt Lake City, Utah COMPENSATION A compensation package to include a base salary between ($70,000 to $80,000) and relocation assistance based on the candidate's situation. OPPORTUNITY: To build a premier genetic testing service laboratory. Myriad Labs offers an opportunity to make a significant change in testing that will result in a meaningful improvement in the quality of early detection and treatment for major diseases. From owner-chromosomes@net.bio.net Thu Feb 01 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!ix.netcom.com!netnews From: opv.lsp@ix.netcom.com(OPV Lifescience Partners ) Newsgroups: bionet.genome.chromosomes Subject: Medical Director for BRCA1 BRCA2 Reference Lab Sought Date: 2 Feb 1996 19:45:07 GMT Organization: Netcom Lines: 142 Message-ID: <4etpk3$hrk@ixnews4.ix.netcom.com> NNTP-Posting-Host: sfo-ca6-10.ix.netcom.com X-NETCOM-Date: Fri Feb 02 11:45:07 AM PST 1996 *************OPV LIFESCIENCE PARTNERS************************ email opv.lsp@ix.netcom.com fax 415 674-1965 ***************************************************************** OPV Lifescience Partners has been retained to identify and place the Medical Director of a reference lab that is working on the cutting edge of predisposition genetics. Please read the position description below and determine whether you are or you know a qualified candidate for this position. We will be conducting this search over the next six weeks so your prompt response is required. All C.V. will be reviewed and appropriate candidates will be contacted. Please email a cover letter and resume to opv.lsp@ix.netcom.com( no attachments will be read) or fax us your resume at 415 674-1965. We appreciate your time and cooperation. *****Note: Do not contact our client directly as we are the exclusive retained firm conducting this position search, any direct contact will not have beneficial results******* _________________________________________________________ POSITION DESCRIPTION Medical Director REPORTS TO: President, Myriad Genetic Laboratories RESPONSIBILITIES: The Medical Director will have responsibility for oversight of Myriad's CLIA-licensed laboratory and its education department. Specifically the Medical Director will be responsible for the results of Myriad's genetic tests; explaining the benefits, educating physicians about the data and providing insurers and government with information for reimbursement considerations. Responding, monitoring, and providing education to clients so as to expand and support reference lab services. Assuring that the company complies with all Federal, State and Local regulations so as not to impede or delay service offerings. This would include the Medical Director's name on the CLIA license. Working with Government, HMO and Insurance providers to obtain reimbursement by showing the cost benefit of testing high risk individuals. Working with opinion leaders on ethical and social issues regarding genetic testing. EDUCATION: Undergraduate degree in life science An advanced degree: MD with pathology/oncology, knowledge of molecular genetics and clinical experience. PERFORMANCE PARAMETERS: Capable of working effectively with his/her peers and subordinates, the research and medical community, customers and suppliers, and regulatory agencies to achieve corporate goals. Ability to explain and develop programs to justify use and reimbursability of genetic testing services CANDIDATE QUALIFICATIONS: The Medical Director will be working on the cutting edge of genetic testing. The candidate must be able to show purpose and value to the new technology while being able to help craft and formulate guidelines for the technology. The candidate must have the depth and experience to work with a variety of third parties who will reimburse the testing services. This is a quickly growing organization that will demand the ability to grow and develop technology that represents a new paradigm in disease detection. Experience working with national accounts including managed care (e.g., HMOs and PPOs), cancer centers, and physician professional corporations, who would obtain services from reference labs, especially esoteric labs. Government and third party payor reimbursement experience. Ten years of professional business experience in the diagnostics or reference laboratory industry or pharmaceutical industry with at least two years of experience with education and reimbursement issues. A technical understanding of genomics and diseases where predisposition factors play a key role. Sensitivity to the social and ethical issues which surround disease predisposition testing. Experience with CLIA Regulations and quality assurance/quality control programs. MEDICAL DIRECTOR PROFILE: Highly energetic, results oriented and dedicated to achieving established and personally defined goals. Excellent interpersonal, motivational and communication skills. Strong leadership skills; exhibit a strong presence and ability to develop and command the respect of others. Maturity to lead and resolve issues in a pressure driven environment. Technical capabilities to understand and incorporate the technologies used in Myriad Labs testing into the factors for success, entry barriers, reimbursement factors, and distribution dynamics. Sensitivity to the social and ethical issues involved in disease predisposition testing. Government and third party payor reimbursement experience. LOCATION: Salt Lake City, Utah COMPENSATION: A compensation package to include a base salary in the low to mid one hundreds and relocation assistance based on the candidate's situation. OPPORTUNITY: To build a leading company in the emerging field of genetic testing that will work with cutting edge technology. Myriad Genetic Laboratories will make a significant change in predisposition testing that will improve the quality of early diagnosis and treatment for major diseases. Improving the lives of countless numbers of people will result in a sense of personal achievement. From owner-chromosomes@net.bio.net Fri Feb 02 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!psinntp!psinntp!psinntp!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.genome.chromosomes Subject: Mouse Chromosome Paints??? DO they exits? Date: 3 Feb 1996 04:21:23 GMT Organization: McGill University Lines: 12 Message-ID: <4euns3$7bl@sifon.cc.mcgill.ca> NNTP-Posting-Host: b-10.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) Doses anyone out there have chromosome specific coatasomes (chromosome paints) for the mouse Or know of a reference for them.... I have been trying to find some in the literature and I am having a hard time. Thanks in advance Graham Dellaire dellaire@odyssee.net From owner-chromosomes@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!col.hp.com!csn!magnus.acs.ohio-state.edu!lerc.nasa.gov!purdue!haven.umd.edu!news.umbc.edu!umbc9.umbc.edu!kao From: "Mr. Jyh-Shyang Kao" Newsgroups: bionet.genome.chromosomes Subject: E. coli linkage map Date: Mon, 5 Feb 1996 15:20:28 -0500 Organization: University of Maryland, Baltimore County Lines: 5 Message-ID: NNTP-Posting-Host: umbc9.umbc.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: kao X-Sender: kao@umbc9.umbc.edu Could anyone out there tell me where I can find the latest version of E. coli K-12 chromosome linkage map? I have the edition 7 (Bachmann, 1987). I am wondering is there a newer version. Thanks in advance. Jay K. From owner-chromosomes@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news From: Ricky Critcher Newsgroups: bionet.genome.chromosomes Subject: Mouse chromosome paints Date: Mon, 05 Feb 1996 08:13:58 +0000 Organization: University of Cambridge, England Lines: 3 Message-ID: <3115BC46.1629@mole.bio.cam.ac.uk> NNTP-Posting-Host: png-mac8.gen.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b6a (Macintosh; I; 68K) Cambio (UK) sell mouse paints which are labelled with Cy 3, biotin, or FITC. Alternativly you could make your own if you can get hold of some monochromosomal mouse hybrids and pcr the mouse chromosome. From owner-chromosomes@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news From: Ricky Critcher Newsgroups: bionet.genome.chromosomes Subject: Re: Mouse Chromosome Paints??? DO they exits? Date: Mon, 05 Feb 1996 08:12:14 +0000 Organization: University of Cambridge, England Lines: 9 Message-ID: <3115BBDE.32DA@mole.bio.cam.ac.uk> References: <4euns3$7bl@sifon.cc.mcgill.ca> <4f3ne5$147@cloner2.ix.netcom.com> NNTP-Posting-Host: png-mac8.gen.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b6a (Macintosh; I; 68K) Cambio (UK) sell mouse specific chromosome paints labelled with Cy3, Biotin, or FITC. Alternativley if you have access to mouse monochromosomal hybrids you can make your own by using pcr to amplify the mouse chromosome. Ricky Critcher Dept.of Genetics Cambridge England From owner-chromosomes@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: hk-miami@ix.netcom.com(HK ) Newsgroups: bionet.genome.chromosomes Subject: Re: Mouse Chromosome Paints??? DO they exits? Date: 5 Feb 1996 01:44:37 GMT Organization: Netcom Lines: 24 Message-ID: <4f3ne5$147@cloner2.ix.netcom.com> References: <4euns3$7bl@sifon.cc.mcgill.ca> NNTP-Posting-Host: ix-mia1-26.ix.netcom.com X-NETCOM-Date: Sun Feb 04 5:44:37 PM PST 1996 In <4euns3$7bl@sifon.cc.mcgill.ca> Graham Dellaire writes: > >Doses anyone out there have chromosome specific coatasomes (chromosome paints) for the mouse >Or know of a reference for them.... > >>I have been trying to find some in the literature and I am having a hard time. > >Thanks in advance >Graham Dellaire >>dellaire@odyssee.net ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Could you make your own? Make chromosomes from mice, and microdissect the one of interest (or have someone do it). Then, make a probe using PCR and biotin-dUTP. See Genomics 6:243-251. 1990. If it can be done with human chromosomes (whole chromosomes or chromosome regions), it can certainly be done with mouse chromosomes. Helene University of Miami From owner-chromosomes@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!swsbe6.switch.ch!surfnet.nl!Hermes.nki.nl!news From: coco@nki.nl (coco) Newsgroups: bionet.genome.chromosomes Subject: Re: Mouse Chromosome Paints??? DO they exits? Date: 6 Feb 1996 17:03:24 GMT Organization: The Netherlands Cancer Institute Lines: 48 Message-ID: <4f81ks$7sl@Hermes.nki.nl> References: <4euns3$7bl@sifon.cc.mcgill.ca> Reply-To: Coco@nki.nl NNTP-Posting-Host: pca64.nki.nl Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 In article <4euns3$7bl@sifon.cc.mcgill.ca>, popa0206@PO-Box.McGill.CA says... > >Doses anyone out there have chromosome specific coatasomes (chromosome paints) for the mouse >Or know of a reference for them.... > > >I have been trying to find some in the literature and I am having a hard time. > > >Thanks in advance > >Graham Dellaire > >dellaire@odyssee.net Yes the do exist. Here is the reference: Nat Genet 9: 369-375 (1995)[95315987] Chromosome specific paints from a high resolution flow karyotype of the mouse. P. Rabbitts, H. Impey, A. Heppell-Parton, C. Langford, C. Tease, N. Lowe, D. Bailey, M. Ferguson-Smith & N. Carter MRC Radiotherapeutics Unit, MRC Centre, Cambridge, UK. Chromosomes from antigen stimulated B-cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus-specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced. Jose From owner-chromosomes@net.bio.net Tue Feb 06 22:00:00 1996 Path: biosci!GALAXY.GOV.BC.CA!PMACLEOD From: PMACLEOD@GALAXY.GOV.BC.CA ("Dr. Patrick Macleod 727-4461") Newsgroups: bionet.genome.chromosomes Subject: Realtime Video/multimedia representations of meiosis/mitosis Date: 7 Feb 1996 10:08:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I0XF33KD6UHV9IIN@mr.gov.bc.ca> NNTP-Posting-Host: net.bio.net I am looking for s source of a brief segment that I can use in expalining meiosis and mitosis to couple in a genetic counselling clinic. I have lots of ideograms, FISH cartoons but I would like something that captures the dynamic aspects of cell divisions. Can anyone help diredt me to a source? From owner-chromosomes@net.bio.net Wed Feb 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!dsi.unimi.it!news.unige.it!news From: mazzocco@sirio.cba.unige.it Newsgroups: bionet.genome.chromosomes Subject: silent mutation Date: Thu, 08 Feb 96 12:53:35 PDT Organization: Univ. of Genoa, Italy Lines: 8 Message-ID: NNTP-Posting-Host: cassiopea.cba.unige.it Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage I'm looking for someone who is able to solve this problem: what's the importance of silent mutation among human pathology? Thank you very much, Katia Mazzocco From owner-chromosomes@net.bio.net Thu Feb 08 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!harbinger.cc.monash.edu.au!bunyip.cc.uq.oz.au!news From: andrea crampton Newsgroups: bionet.genome.chromosomes Subject: microdissection of chromosomes Date: 9 Feb 1996 02:06:46 GMT Organization: university of queensland Lines: 31 Message-ID: <4fea7m$qh7@dingo.cc.uq.oz.au> NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="-------------------------------263842142915020" X-Mailer: Mozilla 1.12(Macintosh; I; 68K) X-URL: news:bionet.genome.chromosomes?ALL This is a multi-part message in MIME format. ---------------------------------263842142915020 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Hi! has anyone tried to use microdissection to isolate a whole chromosome and then use it as a base for a chromosome specfic library????? HOW SUCCESSFUL AND HOW EASY IS MICRODISSECTION WHEN USED FOR SUCH AN APPLICATION................i don't want to dive in and break my neck so to speak......... ---------------------------------263842142915020 Content-Transfer-Encoding: 7bit Content-Type: text/plain >!>!>!>!>!>!>!>!>!>!>!>!>>!>!>!>!>!>!>!>>!>!>!>!>!>!>!>>!>!>!>!>!>! #:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:#:# Newsgroups: bionet.genome.chromosomes Subject: Size of Mouse Genes Date: Fri, 09 Feb 1996 14:23:06 -0600 Organization: Dept. of Physiology-UAB Lines: 3 Message-ID: <311BAD2A.4259@phybio.bhs.uab.edu> NNTP-Posting-Host: phy192.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) I would like to know the size of the entire murine gene of the following: CD3 eta/phi, L14 S-type lectin, TCR delta, TdT, Tenascin, and vav. Where can I find this information? From owner-chromosomes@net.bio.net Sun Feb 11 22:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!in2.uu.net!csn!nntp-xfer-2.csn.net!tali.UCHSC.edu!essex.UCHSC.edu!binstoct From: Teresa Binstock Newsgroups: bionet.molbio.bio-matrix,bionet.molbio,bionet.genome.chromosomes,bionet.general,bionet.cellbiol Subject: RIBOSOME INQUIRY re: S4x and S4y Date: Mon, 12 Feb 1996 03:36:49 -0700 Organization: University of Colorado, Health Sciences Center Lines: 27 Message-ID: References: <311EC8DB.41C6@dancer.biochem.missouri.edu> NNTP-Posting-Host: essex.uchsc.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <311EC8DB.41C6@dancer.biochem.missouri.edu> Xref: biosci bionet.molbio.bio-matrix:706 bionet.genome.chromosomes:1037 bionet.general:19865 bionet.cellbiol:4020 In humans, the X and Y chromsomomes have genes for S4 ribosomal subunits. These genes are homologous but not identical. The most complete symbol for each gene is rpS4x and rpS4y. This means that human males translate mRNA via {one of S4x and one of S4y} whereas human females translate mRNA via {two of S4x}. This sexual dimorphism of S4x and S4y exists independently of gonadal and adrenal hormones. Yet, given all the mRNA processed by humans in their lifetimes and, relatedly, the nearly infinite number of ribosomes used in those translations, the probability seems high that at least a few specific mRNA have sexually dimorphic translation in humans. In other words, at least some genes would lead to differing proteins due to being processed by S4x or by S4y. Should this be the case, then at least some biological sex differences would be independent of gonadal hormones, a finding that would help lay to rest the seemingly outmoded notion of the "bipotential embryo". I would like to receive suggestions regarding how to experimentally determine translation differences between S4x and S4y, and would appreciate sincere replies being sent me directly, and posted to newsgroup only if you so wish. Thank you. Teresa C. Binstock, Researcher Developmental & Behavioral Neuroanatomy Denver CO USA Teresa.Binstock@uchsc.edu From owner-chromosomes@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!PITT.EDU!johnnyv+ From: johnnyv+@PITT.EDU (John Q. Genetics) Newsgroups: bionet.genome.chromosomes Subject: Used"Dupont Genesis" Automated DNA Seqencers FOR SALE. Date: 14 Feb 1996 11:53:02 -0800 Organization: MGB Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net We have 2 - Genesis Sequencers that are in good condition. Please contact John Cardamone at 412-383-9769 at the University of Pittsburgh, Department of Molecular Genetics & Biochemistry, Research Support Facilities. We uill take the best offer. Thank You! John Cardamone jcard+@pitt.edu Also, please visit our web site, http://www.pitt.edu/~rsup From owner-chromosomes@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: Re: Genome Factoids Wanted Date: 15 Feb 1996 00:29:09 GMT Organization: University of Washington, Seattle Lines: 60 Message-ID: <4ftuol$qv0@nntp3.u.washington.edu> References: NNTP-Posting-Host: saul3.u.washington.edu NNTP-Posting-User: roach Here is a relevant report I wrote for internal circulation a few months ago. Note that a few large chunks of sequeunce have been deposited into Genbank since then. September 30, 1995 Here's a run-down on the progress of sequencing the human genome to date (Genbank Release 90.0), considering only DNA (i.e. no mRNA, RNA or ss-DNA): Number of Contigs Greater Than Total Length in these Contigs 40 kb 27 2.60 Mb 30 kb 59 3.74 Mb 20 kb 86 4.40 Mb 10 kb 240 6.50 Mb 5 kb 631 9.15 Mb 1 kb 4115 16.59 Mb Note on table interpretation: There are 59 contigs greater than 30kb, not 27+59. Considering contigs greater than 10 kb as being useful for building the Human Genome sequence, that makes roughly (6.50 Mb)/(3 Gb)=0.22% of the genome sequenced to date. Here are the loci greater than 40kb: HUMTCRB 684973 TCRb 7 HUMRETBLAS 180388 Retinoblastoma 13 HUMFMR1S 152351 HSU07000 152141 HUMIDUR 130000 HUMNEUROF 100849 Neurofibromatosis 17 HUMTCRADCV 97634 TCRa 14 HSABLGR3 84539 HSTCRBV (redundant with HUMTCRB) 77743 HUMHBB 73308 Beta Globin 11 HUMFGLBTK 69363 HUMMMDBC 68468 HUMGHCSA 66495 Growth Hormone and Chorionic Somatomammotropin 17 HSMHCAPG 66109 Major Histocompatibility Complex 6 HSABLGR2 59012 HUMHDABCD 58864 HUMHPRTB 56737 Hypoxanthine Phosphoribosyl Transferase X HUMVITDBP 55136 Vitamin D Binding Protein Gene HUMPKD1GEN 53522 HSG6PDGEN 52173 HSU24498 47934 HSU34879 46610 HSU15177 43599 HSU13369 42999 HSU15422 40573 HUMHDAC 40289 HSL261H12 40198 HUMHDAD 40103 Jared Roach roach@u.washington.edu http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!tank.news.pipex.net!pipex!peer-news.britain.eu.net!warwick!news.wlv.ac.uk!usenet From: Alastair H J Kerr Newsgroups: bionet.genome.chromosomes Subject: studying biotech, SANDWICH job wanted Date: 15 Feb 1996 15:35:09 GMT Organization: University of Wolverhampton, U.K. Lines: 38 Message-ID: <4fvjrd$frp@ccuh.wlv.ac.uk> NNTP-Posting-Host: ma225-bw.wlv.ac.uk Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="-------------------------------293583154230632" X-Mailer: Mozilla 1.2N (Windows; I; 16bit) This is a multi-part message in MIME format. ---------------------------------293583154230632 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii Looking for Sandwich placement from Sept '96. Will fit any role. (see bionet.jobs.wanted for more info) BSc (hons) student Wolverhampton, willing to travel. Thanks. a9481359@wlv.ac.uk Alastair ---------------------------------293583154230632 Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=iso-8859-1 ---------------------------------------------------------------------------- Newsgroup: bionet.genome.chromosomes * microdissection of chromosomes - andrea crampton (31) * silent mutation - mazzocco@sirio.cba.unige.it (8) * Size of Mouse Genes - Edward Walthall (3) * Used"Dupont Genesis" Automated DNA Seqencers FOR SALE. - John Q. Genetics (10) * Re: Genome Factoids Wanted - Jared Roach (60) * Telomeres and chromosome lo - "David Matthes" (29) * RIBOSOME INQUIRY re: S4x and S4y - Teresa Binstock (27) ---------------------------------------------------------------------------- ---------------------------------293583154230632-- From owner-chromosomes@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!BIOMAIL.SJSU.EDU!matthes From: matthes@BIOMAIL.SJSU.EDU ("David Matthes") Newsgroups: bionet.genome.chromosomes Subject: Telomeres and chromosome lo Date: 14 Feb 1996 17:10:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <9602150001.AA07435@chemsuna.sjsu.edu> NNTP-Posting-Host: net.bio.net Telomeres and chromosome loss in hybridomas In my reading about the formation of hybrids between human fibroblasts or lymphocytes and mouse tumor cell lines, I encounter little in the way of explanation for the unequal chromosome loss in the hybrid cells. I recognize that it is advantageous for mapping genes to human chromosomes that most (but not all) of the human chromosomes are lost, but my question is why are the human chromosomes lost and not those of the mouse? I only know of the following two speculations: 1) Mouse chromosomes may replicate more quickly than human chromosomes. Failure to replicate as quickly results in loss. 2) The chromosome loss happens in a similar way as occurs in haploidization in Aspergillis. I'm not sure why or how this would account for the species bias of chromosome loss, but it was mentioned by one author. Does anyone know of work examining how differences in telomeres and telomerase in the two parent cell lines might contribute to the differential chromosome loss? Have reciprocal fusions been made in which human tumor lines have been fused with mouse fibroblasts or lymphocytes? Are mouse chromosomes lost in such hybrids? Thank you. From owner-chromosomes@net.bio.net Wed Feb 14 22:00:00 1996 Path: biosci!biosci!not-for-mail From: dbecker@ix.netcom.com (Daniel M. Becker) Newsgroups: bionet.biology.cardiovascular,bionet.cellbiol,bionet.diagnostics,bionet.general,bionet.genome.chromosomes,bionet.immunology,bionet.metabolic-reg,bionet.microbiology,bionet.molbio.gdb,bionet.molbio.gene-linkage,bionet.molbio.hiv,bionet.molbio.methds-reagents,bionet.molbio.yeast,bionet.virology,bit.listserve.medforum Subject: W. French Anderson seminar, June 1988: did you attend? (legal dispute involving patent rights) Date: 15 Feb 1996 15:58:20 -0800 Organization: Netcom Lines: 23 Sender: biohelp@net.bio.net Distribution: world Message-ID: <3122b3d7.712618@nntp.ix.netcom.com> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biology.cardiovascular:787 bionet.cellbiol:4082 bionet.diagnostics:638 bionet.general:19938 bionet.genome.chromosomes:1043 bionet.immunology:7727 bionet.metabolic-reg:674 bionet.microbiology:4967 bionet.molbio.gdb:427 bionet.molbio.gene-linkage:978 bionet.molbio.hiv:1919 bionet.molbio.yeast:4740 bionet.virology:6227 In connection with a pending legal dispute involving patent rights to human gene therapy, I would like to identify all attendees of a June 1988 seminar given by Dr. W. French Anderson at N.I.H. at which he described the N2-TIL gene therapy protocol (nominally dated June 10, 1988) of which he, R. Michael Blaese, and Steven A. Rosenberg were principal investigators. The Washington Post reported on Wednesday, June 15, 1988 that "[i]n a description of the experiment filed with government officials this week, a research team including Dr. Steven A. Rosenberg of the NCI and Dr. W. French Anderson. . . proposed inserting a 'marker' gene into a newly identified type of cancer-fighting cell and then placing the altered cells into patients. . . . Anderson first described the experiment at a seminar last week at NIH." If you attended this seminar or know someone who did, please contact me via e-mail or snail mail: Daniel M. Becker, M.D., J.D. Pennie & Edmonds 2730 Sand Hill Rd, Suite 300 Menlo Park, CA 94022 dbecker@pennie.com From owner-chromosomes@net.bio.net Fri Feb 16 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: How much of the human genome has been sequenced. (Web Page) Date: 17 Feb 1996 02:34:51 GMT Organization: University of Washington, Seattle Lines: 8 Message-ID: <4g3esb$goo@nntp3.u.washington.edu> NNTP-Posting-Host: saul2.u.washington.edu NNTP-Posting-User: roach I have incorporated my report on how much of the human genome has been sequencedinto a web page at http://weber.u.washington.edu/~roach/human_genome_progress2.html This report is now updated through Genbank Release 92.0 (Dec 15, 1995). From owner-chromosomes@net.bio.net Sat Feb 17 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!omnifest.uwm.edu!omnifest.uwm.edu!not-for-mail From: rbuyan@omnifest.uwm.edu (Ronald A. Buyan) Newsgroups: bionet.genome.chromosomes Subject: Re: Telomeres and chromosome lo Date: 18 Feb 1996 17:11:08 -0600 Organization: Omnifest Lines: 2 Distribution: na Message-ID: <4g8bmc$fs1@omnifest.uwm.edu> NNTP-Posting-Host: 129.89.70.58 There is an Article in the Feb 1996 issue of Scientific American on the subject with some references that may be of some help. From owner-chromosomes@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!agate!hpg30a.csc.cuhk.hk!news.cuhk.edu.hk!newsfeeder.ust.hk!nntp.hk.super.net!news.iij.ad.jp!wnoc-tyo-news!tokyonet.ad.jp!wincgw1!creamy!icspub!odins-suita!aist-nara!t-itou From: t-itou@bs.aist-nara.ac.jp (Takesi Itou) Newsgroups: bionet.genome.chromosomes Subject: Re: E. coli linkage map Message-ID: <4g9ev5$744@fse3.aist-nara.ac.jp> Date: 19 Feb 96 09:13:09 GMT References: Organization: Nara Institute of Science and Technology Lines: 9 NNTP-Posting-Host: genome4.aist-nara.ac.jp Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII X-Newsreader: mnews [version 1.18PL3] 1994-08/01(Mon) > Could anyone out there tell me where I can find the latest version of E. > coli K-12 chromosome linkage map? I have the edition 7 (Bachmann, 1987). Refer to Bachmann, B., (1990) Microbiol. Rev., 54, 130-197. This may be the latest and the last linkage map by Bachmann. The physical map of E. coli K-12 is also available through http://bsw3.aist-nara.ac.jp, but note that these pages are still under construction. From owner-chromosomes@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: e013mr02@area.ba.cnr.it (Mariano ROCCHI) Newsgroups: bionet.genome.chromosomes Subject: chromosome 6 Date: 20 Feb 1996 17:43:12 -0000 Lines: 15 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4gd17g$gmq@mserv1.dl.ac.uk> X-Sender: e013mr02@193.205.35.100 Original-To: biochrom@dl.ac.uk Does anyone have a defective rodent cell line suitable which can be used to select the human chromosome 6 in somatic cell hybrids? Thank in advance Dr. Mariano Rocchi Istituto di Genetica Via Amendola 165/A 70126 Bari - Italy tl +39-80-544.3371 fax +39-80-544.3386 e-mail m.rocchi@area.ba.cnr.it From owner-chromosomes@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!surfnet.nl!sun4nl!xs4all!usenet From: vic2000@easynet.co.uk (-vos-) Newsgroups: bionet.genome.chromosomes Subject: question: XYY-SYNDROM Date: 22 Feb 1996 21:27:53 GMT Organization: 1996 Lines: 7 Message-ID: <4gin4p$2rv@news.xs4all.nl> NNTP-Posting-Host: asd04-18.dial.xs4all.nl X-Newsreader: WinVN 0.92.6+ hello, I want some information about the X-Y-Y chromosome SYNDROME and it's hard to get the right info. Can anybody give me ? Thanks. please email: vic2000@easynet.co.uk From owner-chromosomes@net.bio.net Sun Feb 25 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.genome.chromosomes Subject: Anyone Used Mac Probe for FISH analysis??? Date: 26 Feb 1996 14:40:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602262237.RAA12148@atlas.odyssee.net> NNTP-Posting-Host: net.bio.net I have some graphics files from some Fluorescent In Situ Hyb. analysis. I was using MAC-PROBE to capture and digitize the photos.... Does anyone else have this program? If you do... what kind of graphic files are they and how the hell do you open them (what software). I can't seem to save it as anything other than the native file type the program uses!!!! Help Graham Dellaire McGill University dellaire@odyssee.net From owner-chromosomes@net.bio.net Sun Feb 25 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!surfnet.nl!sun4nl!xs4all!usenet From: foxsong@worldcity.nl (Hans Vosgezang) Newsgroups: bionet.genome.chromosomes Subject: Klinefelter Syndrome ? Date: Mon, 26 Feb 1996 10:21:09 GMT Organization: XS4ALL, networking for the masses Lines: 43 Message-ID: <4gr214$lbq@news.xs4all.nl> NNTP-Posting-Host: dyna1.worldcity.nl X-Newsreader: Forte Free Agent 1.0.82 Hello All! Here is a couple of questions about the Klinefelter syndrome. I am myself are a Klinefelter and a member of the Klinefelter club of Holland. We know allready a lot about these chromosome-deviation but we don’t know how it works somewhere else, so... 1 What _is_ the Klinefelter Syndrome? 2a What does the Klinefelter Syndrome mean to the man with this syndrome? 2b What does it mean to his parents? 2c What does it mean to his partner? 2d What does it mean to his family? 3a What kind of medication is prescribed to treat the side-effect of the Klinefelter syndrome? Meaning: Not an enlisted name, but what the medicine contains and how it is administrated? 3b Why is a specific medicine prescribed to you, your Klinefelter-son, your Klinefelter-partner? 4a Does the Klinefelter syndrome have certain characteristics? 4b Does the Klinefelter syndrome have psychological characteristics? 4c Does the Klinefelter syndrome have psycho-social characteristics? 4d What can be done about these psychological and psycho-social characteristics? 5 Does the Klinefelter syndrome have it’s limitations? 6 Are there aspects of the Klinefelter syndrome you would like to talk about? 7a What scientific studies/resources are there, concerning the Klinefelter syndrome? 7b Are scientists left with unanswered questions after completing their Klinefelter syndrome-studies? Please, if you answer a question... would you answer by number? From owner-chromosomes@net.bio.net Mon Feb 26 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 27 Feb 1996 02:01:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 149 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602271000.CAA14679@net.bio.net> NNTP-Posting-Host: net.bio.net I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We have three sponsors to date with a couple more pending. The process is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we hope to have new and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Statistics are for the four week period from 22 Jan. - 18 Feb. 1996 and usage continues to grow. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5000 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 600 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. ---------------------------------------------------------------------- From owner-chromosomes@net.bio.net Wed Feb 28 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!sgigate.sgi.com!fido.asd.sgi.com!news.neu.sgi.com!news From: Reinhard Schneider Newsgroups: bionet.genome.chromosomes Subject: Yeast genome analysis on a SGI Supercomputer - demonstration project. Date: Thu, 29 Feb 1996 21:58:37 +0100 Organization: EMBL Lines: 67 Message-ID: <3136137D.15FB@embl-heidelberg.de> NNTP-Posting-Host: epca4.neu.sgi.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b6aS (X11; I; IRIX64 6.1 IP21) GeneCrunch: See genome analysis on a supercomputer as it happens. Watch the analysis of genome information live on the WWW, March 4-7, 1996. In a joint demonstration project between Silicon Graphics and scientists from the EMBL-Heidelberg/EMBL-EBI the GeneQuiz software system will analyze more than 6000 protein sequences from the genome of yeast (Saccharomyces cerevisiae) on a 64 processor SGI POWER CHALLENGEarray in just a few days. The project will demonstrate large-scale genome sequence analysis on an almost-complete set of yeast genes. The analysis will be repeated as soon as the international collaborative effort of yeast researchers releases to the public the complete genetic blueprint of yeast, scheduled for later this year. The results on the likely biological function of many new genes will be of interest to biological researchers in both academia and industry. The results will be published on two internet Web sites as the analysis progresses, starting March 4th, 1996: http://genecrunch.sgi.com http://genecrunch.sgi.ch In addition to the biological results of the analysis, the sites will also contain information describing the supercomputing and web technology used. GeneQuiz is an automated system for large-scale genome sequence analysis. It aims at adding value to biosequence information by predicting the biological function of the corresponding genes. GeneQuiz accesses up-to-date protein and DNA databases, derives information by sophisticated search and analysis methods, and interprets any findings in terms of biological function using expert rules. It also predicts three-dimensional structures of protein molecules when these can be inferred by homology . The predicted functions are made available in hyperlinked WWW tables and the predicted coordinates of 3D structures in Protein Data Bank format. GeneQuiz is a collaborative effort between the two EMBL institutes and former EMBL scientists at CNB Madrid, MDC Berlin, and SRI Meno Park. The POWER CHALLENGEarray used for this project is located at the Silicon Graphics European Supercomputing Technology Center in Cortaillod, Switzerland. The POWER CHALLENGEarray consists of 4 POWER CHALLENGE nodes each with 16 R8000 Processors linked together with the very fast HIPPI interfaces. Reinhard Schneider and Georg Casari Biological Structures and Biocomputing program EMBL-Heidelberg Chris Sander European Bioinformatics Institute EMBL-EBI From owner-chromosomes@net.bio.net Thu Feb 29 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.tamu.edu!news.utdallas.edu!news.starnet.net!wupost!ursa.smsu.edu!newsdist.tc.umn.edu!mayonews.mayo.edu!news From: errabolu Newsgroups: bionet.genome.chromosomes Subject: Cells lacking X or 18 chromosomes. Date: 29 Feb 1996 22:56:38 GMT Organization: Mayo Foundation Lines: 7 Message-ID: <4h5av6$f37@tribune.mayo.edu> NNTP-Posting-Host: molebio.mayo.edu Hai Folks, I would like to know if you have come across a human cell line(s) which are negative in chromosome X and 18. I am interested in cell lines which have lost both these chromosomes or tumors whose development and prognosis is affected by the loss of one or both of the chromosomes. Thanks. errabolu@mayo.edu