From owner-chromosomes@net.bio.net Mon Apr 01 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!rzunews.unizh.ch!NewsWatcher!user From: hubscher@vetbio.unizh.ch (Ulrich Hubscher) Newsgroups: bionet.molbio.methds-reagnts,bionet.general,bionet.genome.chromosomes,bionet.molbio.proteins,bionet.molbio.yeast,bionet.virology,bionet.molbio.recombination Subject: ANNOUNCE: EMBO Workshop on Molecular Biology of DNA Replication Date: Tue, 02 Apr 1996 16:39:07 +0100 Organization: Universitat Zurich-Irchel Lines: 46 Message-ID: NNTP-Posting-Host: 130.60.120.18 Xref: biosci bionet.molbio.methds-reagnts:42570 bionet.general:20910 bionet.genome.chromosomes:1104 bionet.molbio.proteins:7493 bionet.molbio.yeast:5039 bionet.virology:6861 bionet.molbio.recombination:153 EMBO WORKSHOP: MOLECULAR BIOLOGY OF DNA REPLICATION September 8 - 13, 1996 Weggis (Luzern), Switzerland Organizers: U. Hubscher, Zurich P. Plevani, Milano S. Spadari, Pavia The main topics which will be covered are: Regulatory mechanisms controlling entry into S-phase; replisome assembly and its functional organization within the nucleus; protein-protein interactions among replication proteins; structural organization of the replisome; connection between DNA replication and other DNA transactions (e.g. repair, transcription). Keynote address: Bruce Stillman Cold Spring Harbor Laboratory New York, USA The registration fee of CHF 850. includes all meals, lodging and social events. A certain amount of the budget will be reserved to partially refund the registration fee to young applicants. Appllcations contalnlng one page abstract shoud be sent as soon as possible but not later than May 31st, 1996 to: EMBO WORKSHOP 96: Universitat Zurich-lrchel, Institut fur Veterlnarbiochemie Winterthurerstrasse 190 CH-8057 Zurich, Switzerland _____________________________________________________________________ Ulrich Hubscher Institut fur Veterinarbiochemie Tel: (41-1)-257-54-72 Universitat Zurich-Irchel Fax: (41-1)-257 59 04 Winterthurerstrasse 190 CH-8057 Zurich Switzerland _____________________________________________________________________ From owner-chromosomes@net.bio.net Tue Apr 02 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!swidir.switch.ch!in2p3.fr!oleane!plug.news.pipex.net!pipex!weld.news.pipex.net!pipex!tank.news.pipex.net!pipex!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: suzric@aol.com (SUZRIC) Newsgroups: bionet.genome.chromosomes Subject: Benign Diffuse Symmetric Lipomatosis (BDSL) Date: 3 Apr 1996 12:41:10 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: root@newsbf02.news.aol.com Message-ID: <4jud7m$kug@newsbf02.news.aol.com> Reply-To: suzric@aol.com (SUZRIC) NNTP-Posting-Host: newsbf02.mail.aol.com Does anyone have any information about Benign Diffuse Symmetric Lipomatosis? It's a disorder which is characterized by a short broad neck with fatty tissue deposits all around, extending to the jaw, onto the front and back of the chest, into the armpits and the upper forearm area. It seems to be relataively rare, but I would be very grateful for any information as to this disorder or perhaps someone to contact about this disorder. Thank you very much. From owner-chromosomes@net.bio.net Wed Apr 03 23:00:00 1996 Path: biosci!.rfhsm.ac.uk!k9mhc From: k9mhc@.rfhsm.ac.uk (Mr Vid Mohan-Ram) Newsgroups: bionet.genome.chromosomes Subject: dog c'some size Date: 4 Apr 1996 00:51:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604040837.JAA20627@rfhsun2.rfhsm.ac.uk> NNTP-Posting-Host: net.bio.net hi what is the size of the canine genome? thanks vid From owner-chromosomes@net.bio.net Wed Apr 03 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: cashug@aol.com (CAShug) Newsgroups: bionet.genome.chromosomes Subject: X-chr YACs Date: 3 Apr 1996 20:37:37 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: root@newsbf02.news.aol.com Message-ID: <4jv951$25f@newsbf02.news.aol.com> Reply-To: cashug@aol.com (CAShug) NNTP-Posting-Host: newsbf02.mail.aol.com Hi: There was a message posted about X-chromosome YACs. I lost the message when my computer bombed. Could you repeat message? I'd appreciate it. Thanks, Chris Shugrue cashug@aol.com From owner-chromosomes@net.bio.net Fri Apr 05 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.reston.ans.net!cs.utexas.edu!swrinde!newsfeed.internetmci.com!in2.uu.net!prodigy.com!usenet From: GKXG23A@prodigy.com (Robert Eisenstaedt) Newsgroups: bionet.genome.chromosomes Subject: Genetics Survey Date: 6 Apr 1996 23:27:14 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 68 Distribution: world Message-ID: <4k6uki$1lqq@usenetp1.news.prodigy.com> NNTP-Posting-Host: innugap8-int.news.prodigy.com X-Newsreader: Version 1.2 I'm doing a survey. I would really like it if you could answer it as truthfully as you can and then send it directly back to me at GKXG23C. Gender: Age: Height: Blood type: Ethnic background: Color of hair: Color of your mother's hair: Color of your father's hair: Color of your sibling(s)'s hair: Color of eyes: Color of mother's eyes: Color of father's eyes: Color of sibling(s)'s eyes: Do you have a widow's peak? (A widow's peak is found along your hairline. It is in the middle of your head and comes to a point that directs your vision toward your nose.) Does your mother have a widow's peak? Does your father have a widow's peak? Does your silbling(s) have a widow's peak? Is your hair naturally curly, wavy, or straight? Is your mother's hair naturally curly, wavy, or straight? Is your father's hair naturally curly, wavy, or straight? Is your sibling(s) hair naturally curly, wavy, or straight? Thanks very much! Remember to send it directly to me at GKXG23C! From owner-chromosomes@net.bio.net Fri Apr 05 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!news.ac.net!imci4!newsfeed.internetmci.com!miwok!usenet From: ivanl@castles.com (Ivan Latinkic) Newsgroups: bionet.genome.chromosomes Subject: Genetics research project for my college class. Need Help with references. Date: 6 Apr 1996 01:53:01 GMT Organization: Sierra College Lines: 15 Message-ID: <4k4ipt$dlj@miwok.nbn.com> NNTP-Posting-Host: rocklin10.castles.com Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.6 Hi, I'm doing a research paper in genetics for my Anthropology class. I'm having troubles finding good resources (recent books and magazine articles). Could anybody recommend me some good books (less than 10 yrs. old) or some magazines that deal a lot with genetics. Specifically, I'm looking for articles that talk about the link between genetics and diseases. I appreciate any help with this. Thank you, Ivan ivanl@castles.com From owner-chromosomes@net.bio.net Sun Apr 07 23:00:00 1996 Path: biosci!GDB.ORG!avoltz From: avoltz@GDB.ORG (Amy Voltz) Newsgroups: bionet.genome.chromosomes Subject: Mammalian Homology and Enzyme Function Data via GDB! Date: 8 Apr 1996 09:15:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: Amy Voltz NNTP-Posting-Host: net.bio.net Mammalian Homology and Enzyme Function Data now available from GDB! The Genome Data Base (GDB) has re-established links from over 1500 GDB human gene entries to the mammalian homology data within the Mouse Genome Database (MGD). While the primary focus of MGD is mouse, their homology data includes information on mammals from the baboon to the wallaby! These data include gene symbols, cytogenetic locations and citations. To access the GDB/MGD homology links, follow the steps outlined below to perform a gene search in GDB, then follow the "Homology" link to MGD. Links to enzyme function data for over 400 protein products of human genes can also be found in GDB. Enzyme data is based on Enzyme Commission (EC) numbers as maintained by the Enzyme nomenclature database at SWISS-PROT. The Enzyme database entries include the reaction catalyzed, cofactor(s), links to the PROSITE database (protein sites and patterns), and detailed SWISS-PROT entries that include protein sequence and links to many other databases. To access mammalian homology and enzyme function data from GDB, start at the GDB homepage (http://gdbwww.gdb.org). Select the "Quick Search" option. Enter the symbol for your favorite gene as the "Genome Object Name", e.g. SOD1, and press "Submit". Choose "Gene SOD1" from the list of query results returned. For mammalian homology data: Choose "Mouse Genome Database: Sod1" from the "Homologies" section of the information returned for the SOD1 gene. For enzyme function data: Choose "Protein: Superoxide Dismutase" from the information returned for the SOD1 gene. (The enzyme function links have been made from the genes via their protein products). The link to the EC database is visible under "Enzyme Links": EC 1.15.1.1 Sincerely, Amy K. Voltz avoltz@gdb.org From owner-chromosomes@net.bio.net Sun Apr 07 23:00:00 1996 Message-ID: <072304Z08041996@anon.penet.fi> Path: biosci!agate!howland.reston.ans.net!EU.net!news.eunet.fi!anon.penet.fi Newsgroups: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,rec.gardens,carleton.clubs.cannabis,sdnet.hemp From: an307269@anon.penet.fi (Alien Anthropologist) X-Anonymously-To: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,rec.gardens,carleton.clubs.cannabis,sdnet.hemp Organization: Anonymous forwarding service Reply-To: an307269@anon.penet.fi Date: Mon, 8 Apr 1996 07:14:13 UTC Subject: Re: (fwd) Botany of Skunk/Northern-Lights/Kush cross (fwd) Lines: 55 Xref: biosci bionet.plants:10847 bionet.genome.chromosomes:1111 rec.gardens:106030 People with a moral problem with a plant, please read no further (get an education while you're at it) Cannabis: Sorry to repost, but I got a couple of confused responses to this post before and half of my post got chopped from the annonymizer. It's not that I'm too curious if it was a polyploid, or even that AFOAF wants to make it one, but more how to reverse a cross. BTW, when you people want to put your clones on ice for a while, what do you usually do? Is it a matter of using giberellic acid to form a male, and crossing your hermaphrodite to get seeds? I'm worried the next generation will be genetically inferior due to inbreeding. See if you can address all the above and below questions for the good of the group, thanks... begin repost: Last year AFOAF had the most amazing hybrid (what they say was a Northern Lights x Skunk hybrid. Unfortunately his/her last clone died, but not before s/he had a chance to cross with a Kush male. My questions stemming (no punn intended) purely from a curiosity standpoint, and a haphazard genetic education are as follows: 1) Why did the plant seem to remain with only 3 blades per leaf, long after reverting from flowering cycle - would it ever have changed back to 5? Much more importantly: 2) Is it possible to regenerate the original strain, either through reciprocal crosses and then subsequently producing an f2 generation by crossing daughter plants (with use of giberillic acid), or any other method? ie., if one raised the seeds and crossed the males with the females, and took the second generation, could s/he expect a normal mendellian ratio, and select for parental types? Would one even be able to select for these phenotypes? Are all the seeds produced on one plant identical genetically or is there variation? As an aside: Is there room for peace and love again in such a sterile world? You may want to step down from your moral high (no punn intended) horse and think of the devistation our civilization is leaving in its wake. Put on an album like Roger Waters _Amused to Death_ while you're at it. There's not much time. Free grass; free yourself; free the world. --****ATTENTION****--****ATTENTION****--****ATTENTION****--***ATTENTION*** Your e-mail reply to this message WILL be *automatically* ANONYMIZED. Please, report inappropriate use to abuse@anon.penet.fi For information (incl. non-anon reply) write to help@anon.penet.fi If you have any problems, address them to admin@anon.penet.fi From owner-chromosomes@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.genome.chromosomes,bionet.molbio.rapd Subject: tobacco mutant seeds Date: Tue, 09 Apr 1996 18:37:04 GMT Organization: Imbb-forth,CREtE, creece Lines: 25 Message-ID: <009A0999.B853B614@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Xref: biosci bionet.genome.chromosomes:1114 bionet.molbio.rapd:1559 Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need to tobacco plants that carry known markers; ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.froth.gr From owner-chromosomes@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!jasper.knox.net!jasper.knox.net!npecsok From: "news.know.net" Newsgroups: bionet.genome.chromosomes Subject: lysosomes Date: Mon, 8 Apr 1996 19:45:52 -0400 Organization: Cornerstone Microsystems Inc., Mt.Vernon Ohio USA Lines: 12 Message-ID: NNTP-Posting-Host: jasper.knox.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I am extremely sorry to cause you any loss of time. I have been studying lysosomes for a little while. Basically briefing myself on the and I have become very interested in the way that the lysosome while containing enzymes that digest organic material do not digest the lysosome and in turn the cell itself. This may turn out to be a very simple question but it is one that has intrigued me for a while. I am sorry if this does not relate to your field of study, but I have tried many different newsgroups without sucess and i have been informed that this might be a good place to go. If you don't have any information on this subject and you know someone that does it would be most kind if you could give me there address thank you for your time NPECSOK@knox.net From owner-chromosomes@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.plants,bionet.genome.chromosomes Subject: tobacco mutant seeds (e mail correction) Date: Tue, 09 Apr 1996 22:21:14 GMT Organization: Imbb-forth,CREtE, creece Lines: 25 Message-ID: <009A09B9.097489F5@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Xref: biosci bionet.plants:10868 bionet.genome.chromosomes:1115 Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need tobacco plants that carry known markers; ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.forth.gr From owner-chromosomes@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: bshomer@ebi.ac.uk (Benny Shomer) Newsgroups: bionet.molbio.methds-reagnts,bionet.immunology,bionet.cellbiol,bionet.general,bionet.genome.arabidopsis,bionet.genome.chromosomes,bionet.microbiology,bionet.molbio.proteins,bionet.plants,fr.bio.biolmol,fr.bio.general,sci.bio.microbiology Subject: mtDNA primers - Call for Marathon!!! Date: 9 Apr 1996 21:48:17 -0700 Organization: EBI - European Bioinformatics Institute Lines: 94 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.molbio.methds-reagnts:42854 bionet.immunology:8490 bionet.cellbiol:4467 bionet.general:21048 bionet.genome.arabidopsis:4435 bionet.genome.chromosomes:1116 bionet.microbiology:5645 bionet.molbio.proteins:7542 bionet.plants:10879 sci.bio.microbiology:3133 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ The PCR Primers database - An update. A call for a mitochondrial DNA primers marathon. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ The PCR primers database is a practical database of fully tested and optimized primers for PCR reactions. The database contains all the data items required to reproduce precisely the reaction conditions of the submitting author and contact details in case of a need. To enhance the rate of information flow into the database and obtain focused interest, I would like to invite colleagues to take a part in a "marathon" of collecting primers that were sucessfully used in PCR amplifications of mitochondrial DNA from various species. This theme has been selected due to a great interest on behalf of many people who enquired about the presence of such primers. If this initiative will be sucessful, other themes will be selected according to need. Please let your colleagues know about the database and about this call, especially those researchers who are off the network or who do not read the bionet newsgroups on a regular basis. Obviousely, submission of any other primers are definitely allways welcome. * Data submissions: The database relies heavily on submissions from users. The importance to the users community will increase with the increase in entry numbers. Users are encouraged to submit primers to the database. Submissions can be made either through the WWW or by using a text based submission form. If you maintain large numbers of primers in a spreadsheet or small database or a simple but organized text file, please contact me. I can process large numbers of entries from almost any format. Summary of access means: ------------------------ The database home page can be accessed directly at: http://www.ebi.ac.uk/primers_home.html The data and documentation can be obtained through anonymous ftp to: ftp.ebi.ac.uk under: /pub/databases/primers/ Searches at the CAOS/CAM server: http://www-srs.caos.kun.nl/srs/srsc Users who have no other means of connection besides email, can now use the EMBL-EBI automatic mail server to retrieve the database files and documents. To start working with the mail server in general, send a message containing the word "HELP" either in the subject line or in the body of message, to: netserv@ebi.ac.uk Here are the commands which are available for the primers database: Here is a summary of all the available commands for the primers database, that can be specified in an email message sent to netserv@ebi.ac.uk ------------------------------------------------------------------------- To obtain send the command ------------------------------------------------------------------------- A general help file HELP PRIMERS The database fields definitions file GET PRIMERS:DEFINITIONS A bogus filled entry as an example GET PRIMERS:EXAMPLE An electronic data submission form GET PRIMERS:SUBMISSION.FORM The actual data file with the entries GET PRIMERS:PRIMERS ------------------------------------------------------------------------- Dear colleague, please do not forget, this database will NOT be created by journal scanning and active searching, but from direct submissions from researchers only. The key to the success of this database is your submissions, so do set aside some spare time and send your primers. Our email: primers@ebi.ac.uk WWW : http://www.ebi.ac.uk/primers_home.html fax : +44 1223 494468 Phone : +44 1223 494437 I highly welcome any questions, corrections, remarks or discussions concerning the PCR Primers database (Email is the most preferred way). Best wishes. Good luck with your PCR experiments. Yours, Benny Shomer Email: bshomer@EBI.ac.uk http://www.ebi.ac.uk/ebi_docs/staff/benny.html From owner-chromosomes@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!howland.reston.ans.net!torn!news.ryerson.ca!hopper.acs.ryerson.ca!ntapiero From: ntapiero@acs.ryerson.ca (Neev Tapiero - CNED/P94) Newsgroups: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,rec.gardens,carleton.clubs.cannabis,sdnet.hemp Subject: Re: (fwd) Botany of Skunk/Northern-Lights/Kush cross (fwd) Followup-To: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,rec.gardens,carleton.clubs.cannabis,sdnet.hemp Date: 10 Apr 1996 17:12:03 GMT Organization: Ryerson Polytechnic University Lines: 31 Message-ID: <4kgq53$19ku@ns2.ryerson.ca> References: <072304Z08041996@anon.penet.fi> NNTP-Posting-Host: hopper.acs.ryerson.ca Xref: biosci bionet.plants:10892 bionet.genome.chromosomes:1118 rec.gardens:106486 : 1) Why did the plant seem to remain with only 3 blades per leaf, long : after reverting from flowering cycle - would it ever have changed back : to 5? that's a genetic question requiring controlled studies. : Much more importantly: : : 2) Is it possible to regenerate the original strain, either through : reciprocal crosses and then subsequently producing an f2 generation by : crossing daughter plants (with use of giberillic acid), or any other method? you'll need the an original NLxSkunk1 plant to start. every generation must be cros bred with NLxS until the undesirable traits are bred out. Put it this way: if you crossed your NLxSxKush with NLxS 100 times (yes, 100 plants) maybe 1 or 2 of them will have the right genetics your looking for. otherwise you'll have to wait 2-8 generations to get what your looking for. your best chance is save time, spend the money at Sensi or HempBC or some other seed co. take care N =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= just grow it and grow it and grow it. From owner-chromosomes@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!galaxy.ucr.edu!library.ucla.edu!info.ucla.edu!ihnp4.ucsd.edu!usc!howland.reston.ans.net!newsfeed.internetmci.com!uwm.edu!newsspool.doit.wisc.edu!wiscnews.wiscnet.net!news From: smic@coredcs.com (Jim Crahan) Newsgroups: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,rec.gardens,carleton.clubs.cannabis,sdnet.hemp Subject: (fwd) Botany of Skunk/Northern-Lights/Kush cross (fwd) Date: Wed, 10 Apr 1996 13:21:12 GMT Organization: University of Wisconsin, Madison Lines: 16 Message-ID: <4kg90a$g7g@wiscnews.wiscnet.net> References: <072304Z08041996@anon.penet.fi> NNTP-Posting-Host: ppp2.coredcs.com Xref: biosci bionet.plants:10884 bionet.genome.chromosomes:1117 rec.gardens:106436 an307269@anon.penet.fi (Alien Anthropologist) wrote: > > People with a moral problem with a plant, please read no further (get an > education while you're at it) > > Cannabis: Just a hunch, But I think you're in the wrong group. Jim Crahan Stevens Point, WI ** I don't do cocaine.... I just like the way it smells. ** From owner-chromosomes@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!torn!ccshst05.cs.uoguelph.ca!wright.aps.uoguelph.ca!yeganehh From: yeganehh@wright.aps.uoguelph.ca (Hassan Mehrabani-Yeganeh) Newsgroups: bionet.genome.chromosomes Subject: How do you make ~John Date: 12 Apr 1996 02:09:36 GMT Organization: University of Guelph Lines: 20 Message-ID: <4kke10$i6t@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: wright.aps.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Hi everybody, I wonder if any of yoy LaTeX experts could tell me how to produce a nmae like ~John. Make sure there is no space between ~ and John. Also ~ should not be put over John. This is useful when you want to write your URL address. Best Regards, Hassan From owner-chromosomes@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!usc!elroy.jpl.nasa.gov!swrinde!gatech!newsfeed.internetmci.com!tank.news.pipex.net!pipex!howland.reston.ans.net!torn!govonca3!usenet From: Marianne Lepa Newsgroups: alt.drugs.pot.cultivation,alt.hemp,bionet.plants,bionet.genome.chromosomes,alt.sustainable.agriculture,carleton.clubs.cannabis,sdnet.hemp Subject: Re: (fwd) Botany of Skunk/Northern-Lights/Kush cross (fwd) Date: Thu, 11 Apr 1996 20:10:40 -0700 Organization: Mendel's Garden Lines: 4 Message-ID: <316DC9AF.5EF3@mail.flarc.edu.on.ca> References: <072304Z08041996@anon.penet.fi> <4kgq53$19ku@ns2.ryerson.ca> NNTP-Posting-Host: 204.41.178.3 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.plants:10910 bionet.genome.chromosomes:1119 Could further posters to this thread please remove rec.gardens from the header? Thank you. From owner-chromosomes@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!MAIL.UMU.SE!hong From: hong@MAIL.UMU.SE (Hong Qian) Newsgroups: bionet.genome.chromosomes Subject: Looking for protein Date: 17 Apr 1996 02:31:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604170928.AA06491@mail.umu.se> NNTP-Posting-Host: net.bio.net Hello everyone out there: I am a Ph D. student working for protein structure determination by NMR method. I had finished calculating one protein structure which contains 90 amino acids. Now I am looking for a new protein. If someone has an over-expressed protein which is suitable for NMR study, I would be very happy working for it. The protein used for NMR structure calculation must fufill the following conditions: 1. Solvable at mili-molar range in water. 2. Stable at room temperature. 3. Molecular weight below 30 kD 4. If it contains more than 70 amino acids, it should be isotopic labelled by nitrogen 15 or both nitrogen-15 and carbon-13. Generally, we need around 10 mg protein for NMR study. Of course, it depends on molecular weight of protein. If anyone interest in this, please contact with me by email. Hong Qian NMR Research Group Department of Organic Chemistry Umea University S- 901 87, Umea Sweden email: hong@indro.chem.umu.se tel: 46-90-166936 fax: 46-90-138885 From owner-chromosomes@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.idt.net!usenet From: D Newsgroups: bionet.genome.chromosomes Subject: conserved 5' UTR Date: 17 Apr 1996 04:58:22 GMT Organization: IDT Corporation Lines: 4 Message-ID: <4l1tpe$alf@News.IDT.NET> NNTP-Posting-Host: n1m27.accesscomm.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) X-URL: news:bionet.genome.chromosomes Can anyone point me to some examples of genes with highly conserved 5'UTRs, preferable mammalian? From owner-chromosomes@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!swrinde!sgigate.sgi.com!nntp-hub2.barrnet.net!news1.digital.com!decwrl!amd!netcomsv!uu4news.netcom.com!netcomsv!uu3news.netcom.com!ixnews1.ix.netcom.com!ix.netcom.com!news From: drachlin@ix.netcom.com(Dana Rachlin) Newsgroups: bionet.molbio.yeast,comp.ai.genetic,bionet.genome.chromosomes Subject: Seeking gene sequence info for fungi Date: 17 Apr 1996 20:45:13 GMT Organization: Netcom Lines: 13 Message-ID: <4l3l8p$qid@dfw-ixnews6.ix.netcom.com> NNTP-Posting-Host: ix-nyc12-30.ix.netcom.com X-NETCOM-Date: Wed Apr 17 3:45:13 PM CDT 1996 Xref: biosci bionet.molbio.yeast:5148 comp.ai.genetic:8010 bionet.genome.chromosomes:1124 Hello, We are interested in finding out about any web sites that contain gene sequence information (either partial fragments, complete genes or genomes) for bacteria and fungi. If you can direct us to any of these we would be very grateful. Please send responses to: drachlin@findsvp.com Thank you kindly in advance for your help! Dana Rachlin From owner-chromosomes@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!usenet From: Rachelle Donn Newsgroups: bionet.genome.chromosomes Subject: exon trapping Date: 17 Apr 1996 14:58:11 GMT Organization: University of Manchester (UK) Lines: 6 Message-ID: <4l30u3$q96@yama.mcc.ac.uk> NNTP-Posting-Host: pc422.ser.man.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) I am trying to understand the basic principles (and some of the details) involved in the technique of exon trapping. Could anyone please help me with information on this subject? Thank you. From owner-chromosomes@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!pasteur.fr!infobiogen.fr!sansgene.genethon.fr!not-for-mail From: bernot@sansgene.genethon.fr (Alain Bernot) Newsgroups: bionet.genome.chromosomes Subject: Re: conserved 5' UTR Date: 18 Apr 1996 11:02:24 +0200 Organization: Genethon -- Human Genome Research Centre Lines: 4 Message-ID: <4l50f0$ljk@sansgene.genethon.fr> References: <4l1tpe$alf@News.IDT.NET> NNTP-Posting-Host: sansgene.genethon.fr To: D I suggest you to read : Duret et al, NAR 21, 2315 (1993) From owner-chromosomes@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!news.epix.net!usenet From: Newsgroups: bionet.genome.chromosomes Subject: dysmorphic syndromes Date: 19 Apr 1996 02:25:02 GMT Organization: epix.net Lines: 5 Message-ID: <4l6thu$bpn@guava.epix.net> NNTP-Posting-Host: shckppp75.epix.net I'm seeking info or direction about dysmorphic syndromes. I'm a nurse caring for a child with dysmorphism. Many genetic consults have been inconclusive. Mother took "Fat Burners" (diet aid) early in first trimester. Family is desperate for more information to learn more about their child. Any assistance would be appreciated. Thanks. (frankj@epix.net) From owner-chromosomes@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!galaxy.ucr.edu!library.ucla.edu!csulb.edu!newshub.csu.net!usc!elroy.jpl.nasa.gov!lll-winken.llnl.gov!fnnews.fnal.gov!stc06.ctd.ornl.gov!usenet From: Sheryl Martin Newsgroups: bionet.molbio.yeast,comp.ai.genetic,bionet.genome.chromosomes Subject: Re: Seeking gene sequence info for fungi Date: 19 Apr 1996 20:36:00 GMT Organization: Lockheed Martin Energy Systems Lines: 5 Message-ID: <4l8tfg$dv7@stc06.ctd.ornl.gov> References: <4l3l8p$qid@dfw-ixnews6.ix.netcom.com> NNTP-Posting-Host: esds22.esd.ornl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; U; 16bit) Xref: biosci bionet.molbio.yeast:5156 comp.ai.genetic:8030 bionet.genome.chromosomes:1127 You might find one of the links on the Virtual Library: Genetics helpful. If anyone has suggestions for links to add to the Genetics Virtual Library page, please send them to me at martinsa@ornl.gov. From owner-chromosomes@net.bio.net Sun Apr 21 23:00:00 1996 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!paladin.american.edu!gatech!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: suzric@aol.com (SUZRIC) Newsgroups: bionet.genome.chromosomes Subject: Benign Diffuse Symmetric Lipomatosis (BDSL) Date: 22 Apr 1996 13:54:18 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 9 Sender: root@newsbf02.news.aol.com Message-ID: <4lgh4a$i5o@newsbf02.news.aol.com> Reply-To: suzric@aol.com (SUZRIC) NNTP-Posting-Host: newsbf02.mail.aol.com Does anyone have any information about Benign Diffuse Symmetric Lipomatosis (BDSL)? I would really appreciate any help you can give me in terms of reference materials and/or suggestions on places to search this topic. I am at a total impasse in my search and am in need of any help you can offer. Thank you very much. From owner-chromosomes@net.bio.net Mon Apr 22 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!swidir.switch.ch!serra.unipi.it!news From: ZITO@CMNS.MNEGRI.IT (Francesco Zito) Newsgroups: bionet.genome.chromosomes Subject: ANNOUNCEMENT (ETRO Working Party on genetics and vascular diseases) May 30-June 1, 1996 Date: 23 Apr 1996 13:18:45 GMT Organization: Consorzio Mario Negri Sud Lines: 27 Sender: -Not-Authenticated-[8706] Message-ID: <4lilbl$f26@serra.unipi.it> NNTP-Posting-Host: journ02.cmns.mnegri.it X-Posted-From: InterNews 1.0.5@journ02.cmns.mnegri.it Xdisclaimer: No attempt was made to authenticate the sender's name. Due to the success of the first European Thrombosis Research Organization (ETRO) Working Party on genetics held in 1995, a second Working Party on "Population Genetics of Hemostatic Risk Factors for Arterial Vascular Disease: Genetic - environmental factor interaction" will be held on May 30 - June 1, 1996 at Consorzio Mario Negri Sud (CMNS), in S. Maria Imbaro Italy. This Working Party, organized by ETRO and CMNS with the support of the E.U., will aim at combining and presenting European expertise in the fields of thrombosis, genetics and epidemiology. For your convenience, we have created a web site at http://www.cmns.mnegri.it/etro where you will find registration and grant forms, together with the preliminary programme and other valuable information. From this web site you will be able to submit grant applications and register on-line. To facilitate interaction, we are inviting participants and other news group subscribers to submit questions, via internet or fax, to speakers prior to the Working Party. Questions can be submitted on-line by visiting our web site at http://www.cmns.mnegri.it/etro. All submitted questions have the possibility of becoming topics for debate during the Working Party or may be published in the proceedings. Please note the deadline for Early Registration is April 30, 1996. From owner-chromosomes@net.bio.net Mon Apr 22 23:00:00 1996 Path: biosci!biosci!not-for-mail From: mourad@cephb.fr (Mourad Sahbatou) Newsgroups: bionet.general,bionet.genome.chromosomes,bionet.molbio.gene-linkage Subject: THE CEPH GENOTYPE DATABASE V8.0 AVAILABLE ON WEB AND FTP SERVERS Date: 22 Apr 1996 21:49:54 -0700 Organization: Fondation Jean Dausset - CEPH Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <4lfv89$oal@vodka.cephb.fr> NNTP-Posting-Host: net.bio.net Keywords: genotype, database, limkage, microsatellite, CEPH Xref: biosci bionet.general:21260 bionet.genome.chromosomes:1130 bionet.molbio.gene-linkage:1038 THE CEPH GENOTYPE DATABASE V8.0 AVAILABLE ON WEB AND FTP SERVERS. The most recent version of The CEPH genotype database (V8.0) is now available to the scientific international community. The database holds over 2,450,000 CEPH family genotypes generated during the last 12 years for some 10,975 polymorphic markers, including more than 7,950 microsatellite markers ( 61% of which are hightly polymorphic). The mean heterozygote frequency of the loci in V8.0 is 0.62, and the mean number of alleles per locus is 6.2, essentially reflecting the large number of microsatellites in the database. In addition to the raw genotypes, the database provides users with linkage file formats of markers and two-point lod scores among all pairs of markers on the same chromosome (3,010,048 two-point lodscores have been computed). You can efficiently retrieve and dump the data from our Web/database interface (http://www.cephb.fr/ceph-genotype-db.html). CEPH anonymous FTP server : ftp.cephb.fr CEPH World Wide Web server: http://www.cephb.fr CEPH database manager : cephdbm@cephb.fr From owner-chromosomes@net.bio.net Tue Apr 23 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!EU.net!news2.EUnet.fr!vodka.cephb.fr!news From: SUSINI Newsgroups: bionet.genome.chromosomes Subject: A LOUER EN CORSE Date: Wed, 24 Apr 1996 17:07:21 +0200 Organization: ceph Lines: 15 Message-ID: <317E43A9.6627@cephb.fr> NNTP-Posting-Host: macyac2.cephb.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) A LOUER A AJACCIO UN STUDIO ENTIEREMENT EQUIPE (CUISINE,SALLE DE BAIN) GRAND BALCON AU BORD DE LA PLAGE AVEC VUE SUR LE GOLFE D'AJACCIO JUILLET 2000 / SEMAINE AOUT 2200/ SEMAINE SEPTEMBRE 2000/SEMAINE MAIL OU 53 72 51 19 (PARIS) 43 04 48 01 From owner-chromosomes@net.bio.net Tue Apr 23 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "KARL KLEINE " Newsgroups: bionet.genome.chromosomes Subject: Complete DNA sequence of the Yeast genome available NOW!!! Date: 24 Apr 1996 15:44:43 +0100 Lines: 152 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4lleor$m6j@mserv1.dl.ac.uk> Original-To: biochrom@dl.ac.uk ====================================================================== The Complete DNA Sequence of S. cerevisiae ====================================================================== The complete sequence of the yeast Saccharomyces cerevisiae strain S288C has been determined in an international collaboration of more than 100 laboratories. By April 24th, the sequence data can be copied from the following ftp sites: Europe ftp.mips.embnet.org (directory /yeast) ftp.ebi.ac.uk America ftp-genome.stanford.edu Japan Riken institute (soon) The contributing consortia are: complete chromosomes partial chromosomes EU (Goffeau) II, III, VII, X, XI IV, XII, XVI XIV,XV St. Louis (Johnston) VIII IV, XVI Sanger (Barrell) IX, XIII IV, XVI McGill (Bussey) I XVI Stanford (Davis) V IV Riken (Murakami) VI CHROMOSOME DNA Coordinator Length Release I H. Bussey, Montreal 230,195 1995 (modif. 4-1996) II H. Feldmann, Munich 813,137 8-1994 (modif. 4-1996) III S. Oliver, Manchester 315,344 12-1991 (modif. 4-1996) IV (I) C. Jacq, Paris IV (II) B. Barrell, Cambridge IV (III) M. Johnston, St. Louis IV (IV) R. Davis, Stanford 1,522,191 4-1996 V R. Davis, Stanford 574,860 1995 (modif. 4-1996) VI Y. Murakami, Tokyo 270,148 1995 VII H. Tettelin, Louvain 1,090,936 4-1996 VIII M. Johnston, St. Louis 562,638 8-1994 IX B. Barell, Cambridge 439,885 1995 X F. Gallibert, Rouen 745,443 10-1995 (modif. 4-1996) XI B. Dujon, Paris 666,448 4-1994 XII (left) J. Hoheisel, Heidelberg XII (right) M. Johnston, St. Louis 1,078,171 4-1996 XIII B. Barell, Cambridge 924,430 1995 XIV P. Philippsen, Basel 784,328 4-1996 XV B. Dujon, Paris 1,091,282 4-1996 XVI (I) A. Goffeau, Louvain XVI (II) H. Bussey, Montreal XVI (IIa) R. Davis, Stanford XVI (III) B. Barrell, Cambridge XVI (IV) M. Johnston, St. Louis 948,061 4-1996 mitochondrial genome (as compiled by de Zamaroczy M. and Bernardi G.) Length: 78,520 nt __________________________________________ Total: 12,136,020 nt 12,057,500 nt (without mitochondrial genome) The yeast genome contains several regions with extensive repeated sequences. These repeats have not been sequenced in their entirety but at least one or two copies have been included in the final sequences. Missing repeated sequences: ~ 100 copies of rDNA repeat(chrXII) each ~9,137 nt = 913,700 nt 2 copies of the ENA2 (chrIV) repeat ~3,885 nt = 7,770 nt ~ 10 copies of the CUP1 repeat (chrVIII) ~ 1,998 nt = 19,980 nt ~ 3-5 copies of Y' elements each ~6,700 nt (chrIV telR 2-3 copies; chrXII telR 1-2 copies) = 26,800 nt ~ 750 nt of telomeric sequence of chrVI = 750 nt ___________________________________________________________________ Total of missing repeated sequences: 969,000 nt The total genome of S.c. S288C therefore contains: 12,136,020 nt + 969,000 nt _______________ 13,105,020 nt NOTE: The strain S288C lacks some of the gene families found in brewery and other industrial strains such as the MEL, MAL and SUC gene families and maybe others. Sequence data released before April 1996 are annotated and the systematic nomenclature has been assigned. Due to the priority of the rapid release of the data to the scientific community, the assignment of the systematic nomenclature for open reading frames has not yet been completed for all 16 chromosomes. In collaboration with the consortia involved in this project these nomenclature will subsequently be added to the information on the WWW pages (see below) and finally included in tables on the FTP sites. (Please do NOT start doing your own nomenclature to avoid unnecessary confusion!) Detailed information on open reading frames, genetic elements, and the graphical display by the GSG (Genome Sequence Graph) are available on the WWW: http://www.mips.biochem.mpg.de Reference information is linked to the S. cerevisiae page for each chromosome. Information on the web includes homology listings and graphical display of the chromosomes. A detailed classification of open reading frames is in progress. H.W. Mewes & K. Kleine Max-Planck-Inst. f. Biochemie MIPS 82152 Martinsried, Germany Tel.: +49 89 8578 2657 FAX : +49 89 8578 2655 email: mewes@mips.embnet.org kleine@mips.embnet.org ========================================================================= The data were assembled (EU part) and edited by MIPS (Max-Planck-Inst. f. Biochemie, Martinsried, Germany). We are greatful for comments, corrections of sequence data, and any contribution to the annotation of the data (to be submitted to : zollner@mips.embnet.org ). Acknowledgement: The ftp-directories contain the proper reference for each chromosome. The following MIPS staff members have contributed to the data evaluation: Yeast sequence processing: K. Kleine, K. Albermann, J. Hani, H. Tettelin (Louvain), A. Zollner Informatics: A. Gleissner, C. Harris (WWW), K. Heumann (HPT/GSG), S. Liebl (XChromo), H.W. Mewes (coordinator), A. Maierl, P. Zaccharia Protein Sequence Databank (annotation): G. Fobo, F. Pfeiffer MIPS is supported by the Max-Planck-Gesellschaft, the Forschungszentrum f. Umwelt u. Gesundheit (GSF), the European Commission, and the BMBF ========================================================================= From owner-chromosomes@net.bio.net Tue Apr 23 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!usenet From: B9510@ibf.hist.no (Torstein Karlsen) Newsgroups: bionet.genome.chromosomes Subject: I have discovered the Z-chromosome Date: 24 Apr 1996 17:56:55 GMT Organization: HiST Lines: 7 Message-ID: <4llq17$hia@dole.uninett.no> NNTP-Posting-Host: pcro38284.stud.hint.no X-Newsreader: WinVN 0.92.6+ I have for a longer time had the feeling that there were more than just the X- and Y-chromosome in pair 24. After a longer research I managed to discover a unknown chromosome in pair 24. This chromosome did only a appear in a few testpersons, which all came from nearby the border next to Russia,near Murmansk I would like to get comments on these discoveries! From owner-chromosomes@net.bio.net Wed Apr 24 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!news.restena.lu!usenet From: John Watson Newsgroups: bionet.genome.chromosomes Subject: Hemophilia in women Date: 25 Apr 1996 10:09:59 GMT Organization: European School Luxembourg Lines: 4 Message-ID: <4lnj1n$sl@caladan.restena.lu> NNTP-Posting-Host: slip1.restena.lu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Does X-linked hemophilia in women really exist or is a double dose of the gene leathal? Is there anyone out there that can give me a clear answer to this question From owner-chromosomes@net.bio.net Wed Apr 24 23:00:00 1996 Path: biosci!rutgers!gatech!gt-news!prism!acmex!gt0619a From: gt0619a@acmex.gatech.edu (Brian) Newsgroups: bionet.genome.chromosomes Subject: question on mutations Date: 25 Apr 1996 16:27:46 GMT Organization: Georgia Institute of Technology Lines: 9 Message-ID: <4lo962$hrv@catapult.gatech.edu> NNTP-Posting-Host: acmex-prism.gatech.edu X-Newsreader: TIN [version 1.2 PL2] I would like to know how often mutations occurr per successful replication of DNA. Is DNA quite effective in replication or is this process often flawed? Brian Harriss gt0619a@prism.gatech.edu (a Civil Engineering student, not bio) From owner-chromosomes@net.bio.net Wed Apr 24 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!pasteur.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!rag3.rz-berlin.mpg.DE!andy From: andy@rag3.RZ-Berlin.MPG.DE (Andrei Grigoriev) Newsgroups: bionet.software,bionet.genome.chromosomes Subject: Physical Mapping Calculators - a couple of java applets Date: 25 Apr 1996 16:24:11 GMT Lines: 10 Message-ID: <4lo8vb$s58@fu-berlin.de> NNTP-Posting-Host: rag3.rz-berlin.mpg.de (141.14.130.162) NNTP-Posting-User: andy X-Access: 16 25 816 X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.software:15302 bionet.genome.chromosomes:1136 Two Java applets, calculating numbers of contigs, etc Perhaps a little too late to appear - they just had to deliver Java a few years ago :) http://www.mpimg-berlin-dahlem.mpg.de/~andy/calc/mapcalc.html You'll need a Java-enabled browser to use those. Feedback is most welcome. Andrei Grigoriev From owner-chromosomes@net.bio.net Wed Apr 24 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!rag2.rz-berlin.mpg.DE!crollius From: crollius@rag2.RZ-Berlin.MPG.DE (Hugues Roest Crollius) Newsgroups: bionet.genome.chromosomes Subject: Re: Hemophilia in women Date: 25 Apr 1996 19:40:01 GMT Lines: 48 Message-ID: <4lokeh$6la@fu-berlin.de> References: <4lnj1n$sl@caladan.restena.lu> NNTP-Posting-Host: rag2.rz-berlin.mpg.de (141.14.130.102) NNTP-Posting-User: crollius X-Access: 16 25 816 In article <4lnj1n$sl@caladan.restena.lu>, John Watson writes: |> Does X-linked hemophilia in women really exist or is a |> double dose of the gene leathal? Is there anyone out there |> that can give me a clear answer to this question |> Hello John, In principle X inactivation is random, therefore a female carrying a mutated gene for haemophilia is not affected. However in some cases X inactivation deviates from randomness in a "tissue specific" manner. This is described as "lyonization" and in these cases, a tissue may contain a much higher proportion of cells carrying a mutated gene on the active X chromosome. If lyonization of the factor VIII (F8) or IX (F9) genes in liver occurs, it will result in a case of female with haemophilia. There is a description of this phenomenon in a book called "Human Gene Mutation" D.N. Cooper and M. Krawczak (1993) in "Human Gene Mutations" (Bios Scientific Publishers ltd, London) pp308-309 Articles reporting cases of female haemophilia are : Ingerslev et a., (1989). Female haemophilia A in a familiy with seeming extreme bidirectional lyonization tendency: abnormal premature X-chromosome inactivation ? Clin. Genet. 35: 41-48 Nisen et al., (1986) The molecular basis of severe hemophilia B in a girl N. Engl. J. Med. 315: 1139-1142 Others are also referenced in the above book chapter. Hope this helps. Hugues Hugues Roest Crollius Abt. Lehrach Max-Planck-Institut fuer Molekulare Genetic Ihnestrasse 73 14195 Berlin Germany e-mail: roest@mpimg-berlin-dahlem.mpg.de From owner-chromosomes@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Mike Cherry Newsgroups: bionet.genome.chromosomes,bionet.molbio.yeast Subject: Yeast Genome BLAST and FASTA Searches Date: 26 Apr 1996 17:58:15 -0700 Organization: Dept Genetics, Stanford University Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <317ED2A0.41C6@genome.stanford.edu> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.genome.chromosomes:1139 bionet.molbio.yeast:5186 Congratulations to the hundreds of individuals that were involved in determining the complete genomic sequence of yeast, and to the thousands of researchers that can now ponder the first sequenced eukaryotic genome. The Saccharomyces Genome Database (SGD) is mirroring the sequence and reference files released by MIPS this morning. SGD is also providing BLAST and FASTA searches against the complete genomic sequence via the WWW. To perform a search against the complete Saccharomyces genome go to the SGD home page and follow the links labelled BLAST or FASTA. http://genome-www.stanford.edu/Saccharomyces/ SGD is currently working to integrate the newly released sequence into its database. Mike J. Michael Cherry Internet: cherry@genome.stanford.edu Department of Genetics Stanford University School of Medicine Medical Center, Room M311 Stanford, California 94305-5120 Voice: 415-723-7541 FAX: 415-723-7016 From owner-chromosomes@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 27 Apr 1996 02:00:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604270900.CAA02538@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-chromosomes@net.bio.net Sat Apr 27 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!hermes.is.co.za!news.gem.co.za!cpt20 From: adrianv@gem.co.za (Adrian Varkel) Newsgroups: bionet.genome.chromosomes Subject: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: 28 Apr 1996 14:09:47 GMT Organization: Global Electronic Messaging Lines: 20 Message-ID: <4m0fth$9qg_003@gem.co.za> NNTP-Posting-Host: cpt17.gem.co.za To: all X-Newsreader: News Xpress Version 1.0 Beta #3 FROM: adrianv@gem.co.za (Adrian Varkel) SUBJECT: HELP // Rates of Ageing : Genetically predetermined or environmentally dependant ? DATE: 28 Apr 1996 13:58:03 GMT ORGANIZATION: Global Electronic Messaging Hello, My name is Leon Varkel and I am studying 1st year medicine at the University of Cape Town in South Africa. I have an assignment of 10-12 pages on the subject : Rates of Ageing, genetically predetermined or environmentally dependant ? I have searched for hours on both the internet and in the university's medical library and have been unable to find any information related to this topic. I have found information on the subject "Ageing" (which is too general), but not "Rates of Ageing". The course which this assignment is for is called 'Human Biology'. This may help readers to understand the type of information I need. If anyone has any information or knows where I could find information on this subject I would appreciate it if you would help me. Thanks Leon From owner-chromosomes@net.bio.net Sun Apr 28 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.genome.chromosomes Subject: What is a good DAPI concentration for F.I.S.H. ? Date: 29 Apr 1996 05:34:14 -0700 Organization: McGill Div. of Experimental Medicine Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <3184B4C4.4210@odyssee.net> NNTP-Posting-Host: net.bio.net Hello all, I just bought a new stock of DAPI and I have no idea what would be a good concentration for FISH (i.e. counterstaining chromosomes). Does anyone one have a good rule of thumb... ie. for time and conc. etc.? As well, what is a good solvent to disolve it in? I think the old stock we had was in PBS... but I am not sure... I have heard others disolve the DAPI in whatever anti-fade reagent they are using. Any and all suggestions are welcome. Graham Dellaire McGill University Experimental Medicine dellaire@odyssee.net or popa0206@po-box.mcgill.ca From owner-chromosomes@net.bio.net Sun Apr 28 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!oleane!in2p3.fr!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!rag2.rz-berlin.mpg.DE!crollius From: crollius@rag2.RZ-Berlin.MPG.DE (Hugues Roest Crollius) Newsgroups: bionet.genome.chromosomes Subject: Re: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: 29 Apr 1996 10:33:52 GMT Lines: 40 Message-ID: <4m25ug$qjq@fu-berlin.de> References: <4m0fth$9qg_003@gem.co.za> NNTP-Posting-Host: rag2.rz-berlin.mpg.de (141.14.130.102) NNTP-Posting-User: crollius X-Access: 16 25 816 Hi Leon! It just happened that I had the following review article on top of the pile: Schachter, F. Cohen, D., Kirkwood, T. (1993) Prospects for the genetics of human longevity. Human Genetics 91:519-526 Hope this helps. Hugues H. Roest Crollius Abt. Lehrach Max-Planck-Institut fuer Molekulare Genetic 73 Ihnestrasse 14195 Berlin Germany e-mail: roest@mpimg-berlin-dahlem.mpg.de |> |> Hello, |> |> My name is Leon Varkel and I am studying 1st year medicine at the University of Cape Town in |> South Africa. I have an assignment of 10-12 pages on the subject : Rates of Ageing, genetically |> predetermined or environmentally dependant ? I have searched for hours on both the internet |> and in the university's medical library and have been unable to find any information related |> to this topic. I have found information on the subject "Ageing" (which is too general), |> but not "Rates of Ageing". The course which this assignment is for is called 'Human Biology'. |> This may help readers to understand the type of information I need. |> |> If anyone has any information or knows where I could find information on this subject I would |> appreciate it if you would help me. |> |> Thanks |> Leon From owner-chromosomes@net.bio.net Sun Apr 28 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!news.sprintlink.net!news.mcn.net!Pjesscanada From: jesscanada@mcn.net (Jess Canada) Newsgroups: bionet.genome.chromosomes Subject: It's time Date: Sat, 27 Apr 96 05:39:37 GMT Organization: JC! Software Lines: 1 Message-ID: <4lru5t$6dj@news.mcn.net> NNTP-Posting-Host: gfpm02-070.mcn.net X-Newsreader: News Xpress Version 1.0 Beta #3 It's time for my afternoon walk.... From owner-chromosomes@net.bio.net Mon Apr 29 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!natinst.com!news-relay.us.dell.com!swrinde!newsfeed.internetmci.com!csn!news-1.csn.net!ub!dsinc!netnews.upenn.edu!brass2.med.upenn.edu!user From: obrien@pharm.med.upenn.edu (:-P) Newsgroups: bionet.genome.chromosomes Subject: Chromosome 5 research centers? Date: Tue, 30 Apr 1996 13:49:47 -0500 Organization: U. of Pennsylvania Lines: 10 Message-ID: NNTP-Posting-Host: brass2.med.upenn.edu I'm trying to find cosmids or YACs specific to chromosome 5. Are there recognized centers of research for this chromosome? If so, will you recommend a contact person to me? Please e-mail me. Thanks. -- Still Trying to Avoid the Drunk Monkey :-Peter From owner-chromosomes@net.bio.net Mon Apr 29 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!natinst.com!news-relay.us.dell.com!swrinde!newsfeed.internetmci.com!csn!news-1.csn.net!ub!dsinc!netnews.upenn.edu!brass2.med.upenn.edu!user From: obrien@pharm.med.upenn.edu (:-P) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome 5 research centers? Date: Tue, 30 Apr 1996 13:51:20 -0500 Organization: U. of Pennsylvania Lines: 16 Message-ID: References: NNTP-Posting-Host: brass2.med.upenn.edu In article , obrien@pharm.med.upenn.edu (:-P) wrote: @--> I'm trying to find cosmids or YACs specific to chromosome 5. Are there @--> recognized centers of research for this chromosome? If so, will you @--> recommend a contact person to me? Please e-mail me. Thanks. @--> I mean human chromosome 5...sorry. -- Still Trying to Avoid the Drunk Monkey :-Peter From owner-chromosomes@net.bio.net Tue Apr 30 23:00:00 1996 Path: biosci!oncor.com!kplaia From: kplaia@oncor.com ("Ken Plaia") Newsgroups: bionet.genome.chromosomes Subject: Chromosome 5 Date: 1 May 1996 08:05:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <9605011450.AA03678@uu11.psi.com> NNTP-Posting-Host: net.bio.net Subject: Time: 10:31 AM OFFICE MEMO Chromosome 5 Date: 5/1/96 Dear Peter, For information centers on Chromosome 5, you can contact the Human Genome Project @ 410-955-9705. They can put you in contact with people working on chromosome 5. Good Luck! --Ken Plaia kplaia@oncor.com