From owner-chromosomes@net.bio.net Wed May 01 23:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!news.ox.ac.uk!news From: mike stacey Newsgroups: bionet.genome.chromosomes Subject: Re: What is a good DAPI concentration for F.I.S.H. ? Date: 2 May 1996 10:53:35 GMT Organization: Dept. of Pharmacology. Oxford University Lines: 10 Message-ID: <4ma47f$kkp@news.ox.ac.uk> References: <3184B4C4.4210@odyssee.net> NNTP-Posting-Host: simlab.pharm.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: dellaire@ODYSSEE.NET X-URL: news:3184B4C4.4210@odyssee.net we use a stock concentration of DAPI at 10mg/ml. from this stock I take 1.5ul into 1ml of antifade solution. The antifade i use is called Vectashield, I can give you details if you want. I spot 10ul of DAPI in antifade onto a slide and drop on a coverslip and seal with nailvarnish. Store your slides in the dark. Hope it goes ok, and good luck. Mike ps I'm trying to do FISH on histological sections, and am having great difficulty. Have you any experience in this area?? From owner-chromosomes@net.bio.net Fri May 03 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!howland.reston.ans.net!news.sprintlink.net!new-news.sprintlink.net!news.dx.net!news From: roland.nero@hroads.com (Downlink) Newsgroups: bionet.genome.chromosomes Subject: Re: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: Sat, 04 May 1996 04:03:09 GMT Organization: The DataXchange Network, Inc Lines: 29 Message-ID: <4mea25$lll@news.dx.net> References: <4m0fth$9qg_003@gem.co.za> NNTP-Posting-Host: ppp23.pm1.hroads.com X-Newsreader: Forte Free Agent 1.0.82 adrianv@gem.co.za (Adrian Varkel) wrote: >FROM: adrianv@gem.co.za (Adrian Varkel) >SUBJECT: HELP // Rates of Ageing : Genetically predetermined or environmentally dependant ? >DATE: 28 Apr 1996 13:58:03 GMT >ORGANIZATION: Global Electronic Messaging >Hello, >My name is Leon Varkel and I am studying 1st year medicine at the University of Cape Town in >South Africa. I have an assignment of 10-12 pages on the subject : Rates of Ageing, genetically >predetermined or environmentally dependant ? I have searched for hours on both the internet >and in the university's medical library and have been unable to find any information related >to this topic. I have found information on the subject "Ageing" (which is too general), >but not "Rates of Ageing". The course which this assignment is for is called 'Human Biology'. >This may help readers to understand the type of information I need. >If anyone has any information or knows where I could find information on this subject I would >appreciate it if you would help me. >Thanks >Leon Let me start out by saying that I am in no way associated with the medical profession, but...have you tried Internet Phone? I have seen some medical discussion groups while using it, if you have the patience to sift through all the non-associated rift raft. From owner-chromosomes@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!OKWAY.OKSTATE.EDU!prade From: prade@OKWAY.OKSTATE.EDU ("Rolf A. Prade") Newsgroups: bionet.genome.chromosomes Subject: First Fungal Genome Workshop - First Announcement Date: 6 May 1996 16:06:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Distribution: world Message-ID: <318E31A8.7B80@okway.okstate.edu> NNTP-Posting-Host: net.bio.net First Fungal Genome Workshop August 19 and 20 1996 Stillwater, OK USA First Announcement The First Fungal Genome Workshop will highlight current research in biology, biotechnology and genome sciences as they are applied to filamentous fungi. The goal is to demonstrate the urgent need of large-scale DNA sequencing in the complex integration process of fundamental research with biotechnology and industry. The workshop will bring together investigators from academia and industry and the discussions will focus on genome technology applied to filamentous fungi. Beginning May 20 visit: http://www.okstate.edu/artsci/micro/ffgw/ffgw.htm for detailed and updated information Speakers: T. Adams (Texas A&M University, USA); M. Adams (The Institute for Genomic Research, TIGR, USA); J. Arnold (University of Georgia, USA); A. Clutterbuck (University of Glasgow, UK); N. Dunn-Coleman ( Genencor International, Inc., USA ); M. Goeden ( University of Wisconsin, USA); J. Hamer, (Purdue University, USA); C. van den Hondel (TNO, NL); M. Johnston (Washington University, USA); K. Kochut (University of Georgia, USA); M. Penalva (CSIC, SP); R. Prade (Organizer - Oklahoma State University, USA); G. Selten ( Gist- Brocades, NL); M. Rey (Novo Nordisk Biotech, USA) B. Roe (University of Oklahoma, US); C. Scazzochio (Universite de Paris-Sud, FR); G. Turner (University of Sheffield, UK). Short Presentations: There is time available for short presentations ( 10 min. ). Interested persons are requested to submit a 1 page abstract of their proposed presentation along with a short resume. All requests must be received no later than July 30, 1996. A committee will review all requests and grantees notified by August 5, 1996. The decision by the review committee will be final. Exhibits & Sponsorship: A limited amount of space is available for exhibiting. If you are interested in exhibiting advertising in the conference documentation or sponsoring a lunch, coffee break or cocktail reception, please call Darlene Brooks at (405) 744 8462. Registration: Application forms, complete and updated workshop program, deadlines and further information can be obtained by addressing one of the following options: Web Site, http://www.okstate.edu/artsci/micro/ffgw/ffgw.htm; e-mail, dbrooks@okway.okstate.edu; FAX (405) 744 6992. or mail: Darlene Brooks Coordinator, Extension Programs Arts & Sciences Extension Oklahoma State University Stillwater, OK 74078. USA. From owner-chromosomes@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!oleane!jussieu.fr!infobiogen.fr!sansgene.genethon.fr!not-for-mail From: bernot@sansgene.genethon.fr (Alain Bernot) Newsgroups: bionet.genome.chromosomes Subject: Re: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: 6 May 1996 08:49:46 +0200 Organization: Genethon -- Human Genome Research Centre Lines: 9 Message-ID: <4mk7ea$7km@sansgene.genethon.fr> References: <4m0fth$9qg_003@gem.co.za> NNTP-Posting-Host: sansgene.genethon.fr To: adrianv@gem.co.za (Adrian Varkel) hi, Concerning the genetically predetermined rate of ageing, you could have a look on : Premature aging gene discovered Science 272 193 (1996) Alain From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!news.sprintlink.net!rebecca!newserve!ub!csn!news-1.csn.net!magnus.acs.ohio-state.edu!lerc.nasa.gov!kira.cc.uakron.edu!neoucom.edu!news.ysu.edu!news.ecn.uoknor.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:29:28 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7A68.57EA@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) CC: bionet.general Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!news.sprintlink.net!rebecca!newserve!ub!csn!news-1.csn.net!magnus.acs.ohio-state.edu!lerc.nasa.gov!kira.cc.uakron.edu!neoucom.edu!news.ysu.edu!news.ecn.uoknor.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:29:19 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7A5F.5DBE@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) CC: bionet.general Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!angis.su.OZ.AU!tim From: tim@angis.su.OZ.AU (Tim Littlejohn) Newsgroups: bionet.genome.chromosomes Subject: Re: definition of "Genome" Date: 7 May 1996 01:15:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <318F7EBE.7272@student.uq.edu.au> NNTP-Posting-Host: net.bio.net Andrea, On Tue, 7 May 1996, Andrea Crampton wrote: > Can anyone give me a good definition of the word genome.there seems to > be some conflicting fews floating around. Does it refer solely to the > haploid chromosome content when refering to eukaryotes or can it > include the diploid content......? Also should mitochondrial DNA be > include in the description of an organisms genome??? (i am of the > bbelief that it is not) The mitochondrial genome should be included as a part of the organism's genome and a genome in its own right. This is because the two are really inseparable: many mitochondrial genes have migrated from the mitochondrial genome into the nuclear genome, making the eukaryotic genome a mosaic in this sense. In Canada there is both an organelle genome program: http://megasun.bch.umontreal.ca/ogmpproj.html and an organelle genome database project: http://megasun.bch.umontreal.ca/gobase/gobase.html which look at organelle genomics. You might find these sites of interest. Cheers, Tim Littlejohn -------------------------------------------------------------------------- | ANGIS- The Australian National Genomic Information Service | | http://www.angis.su.oz.au | | email: tim@angis.su.oz.au Phone/FAX: 61-2-351 2948 | -------------------------------------------------------------------------- From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:47:58 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7EBE.7272@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:47:45 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7EB1.1DC6@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) CC: bionet.general Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!sunsite.doc.ic.ac.uk!hgmp.mrc.ac.uk!news From: Margaret Leversha Newsgroups: bionet.genome.chromosomes Subject: Re: What is a good DAPI concentration for F.I.S.H. ? Date: 7 May 1996 09:45:40 GMT Organization: The Sanger Centre Lines: 21 Message-ID: <4mn644$9d1@mercury.hgmp.mrc.ac.uk> References: <3184B4C4.4210@odyssee.net> NNTP-Posting-Host: t70-barramundi.sanger.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) To: dellaire@ODYSSEE.NET X-URL: news:3184B4C4.4210@odyssee.net Hi Graham, The concentration of DAPI used seems to vary depending on what people want to counterstain. If you're not wanting to see chromosome bands you can use higher concentrations, but banding definition is usually clearer with lower counterstain concentrations. We use an aqueous stock at 1mg/ml, stored in the frig. After FISH, we counterstain the chromosomes for 3-4 minutes in a 0.08mg/ml solution (4ul stock in 50ml 2xSSC), then quickly dehydrate and mount in antifade. We used to make up the DAPI in antifade (Citifluor AF1) but found that the staining intensity drops off pretty quickly - maybe due to the alkaline pH. Simple DAPI banding didn't seem to work this way either (again maybe pH-related). I'd be interested to hear what others have to say about this. Best wishes, Margaret From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!news.sprintlink.net!rebecca!newserve!ub!csn!news-1.csn.net!magnus.acs.ohio-state.edu!lerc.nasa.gov!kira.cc.uakron.edu!neoucom.edu!news.ysu.edu!news.ecn.uoknor.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:29:58 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7A86.6890@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) CC: bionet.general Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Mon May 06 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!news.sprintlink.net!rebecca!newserve!ub!csn!news-1.csn.net!magnus.acs.ohio-state.edu!lerc.nasa.gov!kira.cc.uakron.edu!neoucom.edu!news.ysu.edu!news.ecn.uoknor.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: definition of "Genome" Date: Tue, 07 May 1996 17:29:47 +0100 Organization: University of Queensland Lines: 10 Message-ID: <318F7A7B.55E7@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) CC: bionet.general Hi! Can anyone give me a good definition of the word genome.there seems to be some conflicting fews floating around. Does it refer solely to the haploid chromosome content when refering to eukaryotes or can it include the diploid content......? Also should mitochondrial DNA be include in the description of an organisms genome??? (i am of the bbelief that it is not) Thanks for your input in this matter Andrea From owner-chromosomes@net.bio.net Wed May 08 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!pasteur.fr!jussieu.fr!uvsq.fr!Newsmaster From: Olivier Chocron Newsgroups: bionet.genome.chromosomes Subject: (no subject) Date: 9 May 1996 10:21:18 GMT Organization: Universite de Versailles/St Quentin en Yvelines - France Lines: 17 Message-ID: <4msguu$hbb@soleil.uvsq.fr> NNTP-Posting-Host: pluton.robot.uvsq.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.4 sun4m) X-URL: news:bionet.genome.chromosomes -- Hello, I was wondering in true twins have exactly the same genome or not.. I think the answer is yes as the gametes totally combine to yield a new cell which will divide after. Does someone have an answer? Yours, +---------------------------------------------------------------+ o o | Chocron Olivier , Laboratoire de Robotique de Paris | \___/ |// | 10-12 Av. de L'Europe 78140 Velizy-Villacoublay FRANCE | [o o] \| | Tel : (33) (1) 39.25.49.58 FAX: (33) (1) 39.25.49.67 | [ = ] \ | email: chocron@robot.uvsq.fr | || / +---------------------------------------------------------------+ / \====/ From owner-chromosomes@net.bio.net Wed May 08 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.genome.chromosomes Subject: Identical Twins Q? From Olivier Chocron Date: 9 May 1996 05:45:22 -0700 Organization: McGill Div. of Experimental Medicine Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <3191E64F.511D@odyssee.net> NNTP-Posting-Host: net.bio.net Hello Olivier, Not to throw too much of a wrench in the works I would like to just say that even in you and I, our genome is not the "exact" same in all our cells. It turns out that through somatic mutations, some small changes can occur so that the complement of DNA in every cell in your body could differ slightly. Sometimes this is quite evident when there is loss of heterozygosity and recessive disease phenotypes are uncovered (ex. Rb gene). A different process of change that can occur is X mosaicism in females where you have a stochaic inactivation of one X or the other which then effects what allels are expressed in those cells. So even if two embryos are derived from the same egg, as in true twins, I don't think they would have the "exact" same genotype. Small mutations can creep in, many not even apparent as they could occur in the millions of bases of repetitive sequence. The earlier the change the more cells of the embryo contain the change and so on. As far as a differences in outward phenotype or appearance between the twins, we again enter the debate on the extent that physics affects development vs. biology. Meaning that slight changes in physical forces acting on the embryo and during development may affect the outcome of tissue modeling etc. Such epigenetic influences are hard enough to envision never mind model. Just my two bits G. Dellaire From owner-chromosomes@net.bio.net Wed May 08 23:00:00 1996 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in1.uu.net!sangam!iitb!powai!powai.cc.iitb.ernet.in!mmrajan From: "Dr.M.M.Rajan" Subject: Mosquito cell Lines! Sender: news@powai.cc.iitb.ernet.in (news system user) Message-ID: Date: Thu, 9 May 1996 13:13:32 GMT Content-Type: TEXT/PLAIN; charset=US-ASCII Mime-Version: 1.0 Organization: Computer Centre, Indian Institute of Technology, Bombay Lines: 41 From mmrajan@cc.iitb.ernet.in Thu May 9 18:03:03 1996 Date: Thu, 9 May 1996 17:58:02 +0530 From: "Dr.M.M.Rajan" Subject: Mosquito cell lines! Dear Newsgroup reader, Hi! One of my colleagues, Dr.Supriya Kshirsagar, in the Molecular Biology Division is working on mitochondrial dna. She would like to know where she could get mosquito genomic library of any of the species (of the genera Anopheles, Aedes, Culex) any stage (larvae to adult). Anybody with knowledge or information about this, may I please request them to write back or contact: cell@nccs.ernet.in Thanking in anticipation, Yours M.M.Rajan. ---------------------------------------------------------------------------- Dr.M.M.Rajan, M.B.,B.S., Doctoral Research Scholar - Biomedical Engineering, School of Biomedical Engineering, Indian Institute of Technology, Bombay - 400 076. email : mmrajan@cc.iitb.ernet.in At present at: National centre for Cell Sciences Pune - 411 029 cell@nccs.ernet.in ----------------------------------------------------------------------------- From owner-chromosomes@net.bio.net Thu May 09 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!netnews2.nwnet.net!news.u.washington.edu!news.alt.net!news1.alt.net!news.aa.net!ratty.wolfe.net!usenet From: Rhee Newsgroups: bionet.genome.chromosomes Subject: Huntington's Disease Date: Thu, 09 May 1996 17:45:09 +0000 Organization: Wolfe Internet Access, L.L.C. Lines: 8 Message-ID: <31922F25.6273@wolfenet.com> NNTP-Posting-Host: yak-ts1-p56.wolfenet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) I'm working on my extended essay in biology to earn my IB diploma and I was wondering if anyone out there has any information on Huntinton's and the genetics behind it, its place on the genome, etc. I'd appreciate any information, except the type that corrects my grammar, spelling, and punctuation. Cheers, Pollyanna Rhee From owner-chromosomes@net.bio.net Thu May 09 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!newsfeed.internetmci.com!in2.uu.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: How Much of the Human Genome has been Sequenced? (Web Page) Date: 10 May 1996 06:31:45 GMT Organization: University of Washington, Seattle Lines: 20 Message-ID: <4munsh$59i@nntp3.u.washington.edu> NNTP-Posting-Host: saul5.u.washington.edu NNTP-Posting-User: roach I have just updated my "How Much of the Human Genome has been Sequenced?" web page to include statistics through Genbank Release 94.0. This puts us at 0.39% of the human genome sequenced to date. On the other hand, this is double what it was five months ago. http://weber.u.washington.edu/~roach/human_genome_progress2.html --------------------------------------------------- Jared C. Roach Department of Molecular Biotechnology University of Washington, Room K354 Box 357730 Seattle, WA 98195 phone 616-4536 FAX 685-7301 roach@u.washington.edu http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Thu May 09 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!oleane!in2p3.fr!univ-lyon1.fr!jussieu.fr!uvsq.fr!Newsmaster From: Olivier Chocron Newsgroups: bionet.genome.chromosomes Subject: Re: Identical Twins Q? From Olivier Chocron Date: 10 May 1996 17:55:56 GMT Organization: Laboratoire de Robotique de Paris Lines: 25 Message-ID: <4mvvvc$3dj@soleil.uvsq.fr> References: <3191E64F.511D@odyssee.net> NNTP-Posting-Host: pluton.robot.uvsq.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.4 sun4m) To: dellaire@ODYSSEE.NET X-URL: news:3191E64F.511D@odyssee.net -- Hi all, Thanks to (Graham Dellaire) for his answer about true twins. Meanwhile, I have three more questions to ask to the genetic comunity: 1) how many genes are found in each chromosome (let say in haploid form) in mankind genotype? Are all the human genes known? 2) in how many distincts alleles each human gene can be expressed? I learned two but it was in basic genetics (drosophilia and bins). 3) Can we manipulate human embryos to make two equal babies as for bovins? Are all genes equally important or can we loose some without dramatic results? Thanks, Olivier +---------------------------------------------------------------+ o o | Chocron Olivier , Laboratoire de Robotique de Paris | \___/ |// | 10-12 Av. de L'Europe 78140 Velizy-Villacoublay FRANCE | [o o] \| | Tel : (33) (1) 39.25.49.58 FAX: (33) (1) 39.25.49.67 | [ = ] \ | email: chocron@robot.uvsq.fr | || / +---------------------------------------------------------------+ / \====/ From owner-chromosomes@net.bio.net Fri May 10 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!uunet!in2.uu.net!minerva.ibernet.es!newsfeed.tip.net!peroni.ita.tip.net!news.vol.it!news From: tramuto@mbox.vol.it Newsgroups: bionet.genome.chromosomes Subject: Transactivation of HIV1 Date: Thu, 09 May 1996 22:11:33 +0200 Organization: Video On Line Lines: 15 Message-ID: <31925175.6611@mbox.vol.it> NNTP-Posting-Host: molpa14.vol.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Dear Collegues, I am intersted in transactivation of HIV1 LTR by TNF(alfa) and PMA; particularly I need an experimental protocol for epithelial cells (HeLa) transfected with a plasmid containing LTR-CAT. If someone is working in this field, please, contact me. Thanks Dr. TRAMUTO Fabio E-mail: tramuto@mbox.vol.it Dept of Hygiene and Microbiology University of Palermo Via del Vespro, 133 I-90127 - Palermo - Italy From owner-chromosomes@net.bio.net Fri May 10 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!swrinde!newsfeed.internetmci.com!newsfeed.direct.ca!news From: jportela@direct.ca Newsgroups: bionet.genome.chromosomes Subject: Re: Huntington's Disease Date: Sat, 11 May 1996 16:03:55 GMT Organization: Canada Internet Direct, Inc. Lines: 19 Message-ID: <4n2d8s$osj@aphex.direct.ca> References: <31922F25.6273@wolfenet.com> NNTP-Posting-Host: van-as-11c08.direct.ca X-Newsreader: Forte Free Agent 1.0.82 Dear Rhee: I am sure you probably already know about Dr. M Hayden. He is responsible for the identification, sequencing and implementation of the diagnostic test for Huntington's disease. I sure you could find all you need to know by following a search path through biological abstracts. Dr. Hayden is currently at UBC in British Columbia in the Dept of Medical Genetics. >I'm working on my extended essay in biology to earn my IB diploma and >I was wondering if anyone out there has any information on Huntinton's >and the genetics behind it, its place on the genome, etc. I'd >appreciate any information, except the type that corrects my grammar, >spelling, and punctuation. >Cheers, >Pollyanna Rhee From owner-chromosomes@net.bio.net Fri May 10 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rcjohnsen@aol.com (Rcjohnsen) Newsgroups: bionet.genome.chromosomes Subject: Re: Huntington's Disease Date: 11 May 1996 17:20:38 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 22 Sender: root@newsbf02.news.aol.com Message-ID: <4n30b6$ibj@newsbf02.news.aol.com> References: <4n2d8s$osj@aphex.direct.ca> Reply-To: rcjohnsen@aol.com (Rcjohnsen) NNTP-Posting-Host: newsbf02.mail.aol.com Pollyanna You can find out about Huntington by going to OMIM at the address McKusick's Mendelian inheritance in man http://www3.ncbi.nlm.nih.gov/omim/ and enter the key word in the search engine. It will retrieve the latest and greatest about the disease; clinical, diagnosis, the gene location, references. Alternatively, go to the Web Crawler http://webcrawler.com/ and enter Huntington Disease and the search engine will retrieve over 10,000 documents. Thne you can go to Yahoo at http://www.yahoo.com/ and try the same thing or go to SEARCH ENGINE FOR SCI http://www.chem.msu.su/eng/comparison.html All addresses should yield lots of info Good luck. Rcjohnsen@aol.com From owner-chromosomes@net.bio.net Sun May 12 23:00:00 1996 Newsgroups: bionet.genome.chromosomes Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!sgigate.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.ner.bbnplanet.net!cpk-news-hub1.bbnplanet.com!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!news From: "Pablo A. Scolnik" Subject: E. coli genome blots Content-Type: text/plain; charset=us-ascii Message-ID: <1996May13.153557.19499@es.dupont.com> Sender: news@es.dupont.com (USENET News System) Nntp-Posting-Host: scolnipa.es.dupont.com Content-Transfer-Encoding: 7bit Organization: dupont Mime-Version: 1.0 Date: Mon, 13 May 1996 15:35:57 GMT X-Mailer: Mozilla 1.1 (Macintosh; U; 68K) X-Url: news:bionet.genome.chromosomes Lines: 18 I need to set up some global gene expression experiments in E. coli using labeled RNA against genome grids. I've read Shuang-En Chuang et al. (J. Bact. '93), and they use the Kohara phages. They also mention that blots are available from Takara Shugo. I wonder whether: 1. someone has used the commercial blots and they are O.K. 2. as a consequence of the genome sequencing project we have better alternatives available, i.e. single-gene probes that can be arrayed at high density. Thank you Pablo A. Scolnik scolnipa@esvax.dnet.dupont.com From owner-chromosomes@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.genome.chromosomes Subject: Re: (no subject) Date: Mon, 13 May 1996 17:23:18 -0700 Organization: Lawrence Livermore Lines: 12 Message-ID: <3197D276.589EB32B@mackiller.llnl.gov> References: <4msguu$hbb@soleil.uvsq.fr> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; Linux 1.1.72 i486) Olivier Chocron wrote: > > -- Hello, > > I was wondering in true twins have exactly the same genome or not.. > I think the answer is yes as the gametes totally combine to yield > a new cell which will divide after. > > Does someone have an answer? True twins? (Identical, fraternal half Identical) Telomeric sequences conserved or not? From owner-chromosomes@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!csn!nntp-xfer-1.csn.net!magnus.acs.ohio-state.edu!ts30-1.homenet.ohio-state.edu!user From: snyder.9@osu.edu (Pam Snyder) Newsgroups: bionet.genome.chromosomes Subject: Re: Identical Twins Q? From Olivier Chocron Date: Sat, 11 May 1996 21:49:17 -0500 Organization: Just me Lines: 40 Message-ID: References: <3191E64F.511D@odyssee.net> <4mvvvc$3dj@soleil.uvsq.fr> NNTP-Posting-Host: ts30-1.homenet.ohio-state.edu In article <4mvvvc$3dj@soleil.uvsq.fr>, Olivier Chocron wrote: > -- Hi all, > > Thanks to (Graham Dellaire) for his answer about true twins. > Meanwhile, I have three more questions to ask to the genetic comunity: > > 1) how many genes are found in each chromosome (let say in haploid form) > in mankind genotype? The estimate is around 100,000 Are all the human genes known? NO > > 2) in how many distincts alleles each human gene can be expressed? > I learned two but it was in basic genetics (drosophilia and bins). humans can carry no more that 2 alleles at any loci(spot on a chromosome). The number of alleles that actually exist in a population can be very numerous > > 3) Can we manipulate human embryos to make two equal babies as for bovins? When attempts have been made (and it not something that is easy to get approval for) the cells did not survive beyond 3 divisions > > Are all genes equally important or can we loose some without dramatic results? men typically have one one copy of each gene on the X chromosome and do fine, If however that one copy is altered in any way they are screwed. Some autosomal (nonsex chromosomal) genes are like this as well. Any disease that is called recessive requires two abberant copies to cause the disease. One missing or altered allele is not sufficient to cause the symptoms of the disease. > > Thanks, > > Olivier > +---------------------------------------------------------------+ o o > | Chocron Olivier , Laboratoire de Robotique de Paris | \___/ |// > | 10-12 Av. de L'Europe 78140 Velizy-Villacoublay FRANCE | [o o] \| > | Tel : (33) (1) 39.25.49.58 FAX: (33) (1) 39.25.49.67 | [ = ] \ > | email: chocron@robot.uvsq.fr | || / > +---------------------------------------------------------------+ / \====/ From owner-chromosomes@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!usenet From: Chris Barry Newsgroups: bionet.genome.chromosomes Subject: Re: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: Tue, 14 May 1996 10:28:59 -0700 Organization: Lawrence Livermore Lines: 19 Message-ID: <3198C2DB.3A3E547E@mackiller.llnl.gov> References: <4m0fth$9qg_003@gem.co.za> <4mea25$lll@news.dx.net> NNTP-Posting-Host: mackiller.llnl.gov Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; Linux 1.1.72 i486) > >My name is Leon Varkel and I am studying 1st year medicine at the University of Cape Town in > >South Africa. I have an assignment of 10-12 pages on the subject : Rates of Ageing, genetically > >predetermined or environmentally dependant ? I have searched for hours on both the internet > >and in the university's medical library and have been unable to find any information related > >to this topic. I have found information on the subject "Ageing" (which is too general), > >but not "Rates of Ageing". The course which this assignment is for is called 'Human Biology'. > >This may help readers to understand the type of information I need. > > >If anyone has any information or knows where I could find information on this subject I would > >appreciate it if you would help me. > > >Thanks > >Leon I suggest you try doing a search under "telomeric sequences". I think a lot of research has been done with regard telomerase and cellular ageing. That should keep you occupid for a while. Chris From owner-chromosomes@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!rutgers!oitnews.harvard.edu!news.dfci.harvard.edu!1002 From: mohan_natesan@dfci.harvard.edu (Mohan) Newsgroups: bionet.genome.chromosomes,bionet.general,bionet.immunology,bionet.cellbiol Subject: Help//Obtaining CXS mice RI strains Date: 14 May 1996 18:54:27 GMT Organization: Dana-Farber Cancer Institute Lines: 13 Sender: -Not-Authenticated-[4156] Message-ID: <4nakt3$3he@netman-mel.dfci.harvard.edu> NNTP-Posting-Host: mac1002.dfci.harvard.edu X-Posted-From: InterNews 1.0@netman-mel.dfci.harvard.edu. Xdisclaimer: No attempt was made to authenticate the sender's name. Xref: biosci bionet.genome.chromosomes:1178 bionet.general:21670 bionet.immunology:8818 bionet.cellbiol:4697 I am trying to obtain CXS mouse RI strains for my study (BALB/c X STS/A). I would appreciate if someone can tell me a source in USA. I have tried all the companies and Netherlands Cancer Center which originally devleoped these strains. Thanks. ---------------------------------------------------------------------- Mohan N D1002 DFCI 44 Binney St Boston MA 02115 mohan_natesan@dfci.harvard.edu From owner-chromosomes@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news.moneng.mei.com!news.ecn.bgu.edu!vixen.cso.uiuc.edu!news.uoregon.edu!newsxfer2.itd.umich.edu!uunet!in2.uu.net!news.iadfw.net!dal22-24.ppp.iadfw.net!user From: burke@airmail.net (Burke Squires) Newsgroups: bionet.genome.chromosomes Subject: Re: help // rates of ageing : genetically predetermined or environmentally dependant ? Date: 15 May 1996 03:33:17 GMT Organization: St. Monica School Lines: 28 Message-ID: References: <4m0fth$9qg_003@gem.co.za> <4mea25$lll@news.dx.net> <3198C2DB.3A3E547E@mackiller.llnl.gov> NNTP-Posting-Host: dal22-24.ppp.iadfw.net In article <3198C2DB.3A3E547E@mackiller.llnl.gov>, Chris Barry wrote: > >I suggest you try doing a search under "telomeric sequences". I think a >lot of research has been done with regard telomerase and cellular >ageing. That should keep you occupid for a while. > >Chris I will be returning to this in the are of telomere/telomerase soon. As alternatives to that spelling try telomere, and telomerase (an enzyme that rebuilds telomeres!) as well as stuff on apotosis, or programmed cell death. Burke Squires -- ====================================================== = Burke Squires, B.S. ;-) = = St. Monica School Dallas, Texas = = Computer Teacher/Technical Coordinator = = BurkeS@eWorld.com = ====================================================== = When people tell me there is strength in numbers = = I remind them that one (1) is a number! = = - Burke Squires, 1992 = ====================================================== From owner-chromosomes@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.genome.chromosomes Subject: tobacco mutant seeds Date: Fri, 17 May 1996 17:20:26 GMT Organization: Imbb-forth,CREtE, creece Lines: 32 Message-ID: <009A276B.4F65A29E@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need tobacco plants that carry known markers; especially ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Please note that this message had to be reposted because of a failure to our VAX system that didnt allow us to recieve any responses. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.forth.gr From owner-chromosomes@net.bio.net Fri May 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.hooked.net!nh1.well.com!usenet From: newslist@gina.com (Christian Carollo) Newsgroups: bionet.genome.arabidopsis,bionet.genome.chromosomes,bionet.immunology Subject: New Reference Web Site Date: Mon, 13 May 1996 22:59:58 GMT Organization: The WELL Lines: 15 Message-ID: <4n8du0$fbg@nh1.well.com> NNTP-Posting-Host: 204.140.244.217 X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.genome.arabidopsis:4568 bionet.genome.chromosomes:1183 bionet.immunology:8864 Hello, A new medical research reference site has recently opened up on the web. The site is owned and operated by Allegheny-Singer Research Institute. ASRI serves as the research arm of the Allegheny Health, Education and Research Foundation (AHERF). ASRI is a medical research organization with more than 100 scientists who conduct research in five major areas -- human genetics, the neurosciences, oncology, cardiovascular/pulmonary and musculoskeletal or orthopedic. Their web address is: http://www.asri.edu. For further information please speak with Angela Burrows, at Burrows@asri.edu. From owner-chromosomes@net.bio.net Fri May 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!sgigate.sgi.com!nntp.coast.net!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: djkjd@aol.com (Djkjd) Newsgroups: bionet.genome.chromosomes Subject: Re: Identical Twins Q? From Olivier Chocron Date: 18 May 1996 01:00:06 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <4njlgm$16d@newsbf02.news.aol.com> References: <4mvvvc$3dj@soleil.uvsq.fr> Reply-To: djkjd@aol.com (Djkjd) NNTP-Posting-Host: newsbf02.mail.aol.com Hello. Can you tell me where to locate information about chromosomal abnormalities and spontaneous abortion (miscarriage)? I'd appreciate your help. e-mail address is: djkjd@aol.com From owner-chromosomes@net.bio.net Sun May 19 23:00:00 1996 Path: biosci!VMS.UCC.OKSTATE.EDU!prade From: prade@VMS.UCC.OKSTATE.EDU ("Rolf A. Prade") Newsgroups: bionet.genome.chromosomes Subject: Visit The First Fungal Genome Workshop HP Date: 20 May 1996 14:10:01 -0700 Organization: Oklahoma State University Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <31A08B6D.76A9@vms.ucc.okstate.edu> Reply-To: prade@vms.ucc.okstate.edu NNTP-Posting-Host: net.bio.net 1st FGW The First Fungal Genome Workshop Stillwater, OK - August 19 & 20 Program and Registration http://www.okstate.edu/artsci/micro/ffgw/ffgw.htm ---*--- From owner-chromosomes@net.bio.net Mon May 20 23:00:00 1996 Path: biosci!agate!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.genome.chromosomes Subject: Chromosomal Pet Peeve Date: 21 May 1996 04:15:11 GMT Organization: UCSF, ITS Lines: 28 Message-ID: <4nrg0f$15lc@itssrv1.ucsf.edu> NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] Colleagues I continue to study very hard for the Genetics Boards exam and find that I have again stumbled on the difficult issue of how many chomosomes (and how much DNA) is present when during meiosis. This is a favorite question of the exam-gods, but I always seem to over interpret (probably from doing a Ph.D. in spermatogenesis). At any rate, the issue I would like your help with is this: How can all the textbooks continue to maintain that the number of chromosomes during MI of Meiosis and M2 of Meiosis (until completion) always remains at 2N? At this point they mostly admit that the amount of DNA goes up to 4C before leptotene, but how can you have 4C,2N? Apparently, the textbook makers think that one tetrad equals one chromosome? At any rate, are they really counting centromeres when they say chromosomes?? If you can't figure out what the hell I'm writing about, you are welcome to look at Vogel and Motulsky, page 36, or just try to answer the problems in Thompson and Thompson, page 30, problems 4a and 4b (I think the answers at the end of the book are wrong; but your opinion is welcomed). Bert Gold, Ph.D. UCSF From owner-chromosomes@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!quagga.ru.ac.za!und.ac.za!news.unp.ac.za!zoology.unp.ac.za!Bonkewitzz From: Bonkewitzz@zoology.unp.ac.za (Americo.Bonkewitzz) Newsgroups: bionet.genome.chromosomes Subject: Meiosis speed & non-viables gametes Date: Wed, 22 May 1996 07:38:51 GMT Organization: University of Natal , Pietermaritzburg, South Africa Lines: 18 Message-ID: NNTP-Posting-Host: 143.128.64.122 Hi, I am trying to find out whether the speed of Meiosis could produce non- viables gametes or "bad gametes" (gametes with chismata-associated errors, lack of DNA reparation, etc). There are many animal due to sperm competition that are able to produce a tremendous amount of sperm in a short period of time, wouldn't this lead to errors in crossing overs ? Is it the amount of gametes to produce or the speed at which is produce what causes non-viability or "bad gametes" ? Has anyone got any reference or could anyone give an expert opinion ? Thank you in advance A. N. Bonkewitzz bonkewitzz@zoology.unp.ac.za bonkewit@unpsun1.cc.unp.ac.za From owner-chromosomes@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!agate!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.genome.chromosomes Subject: Re: Meiosis speed & non-viables gametes Date: 22 May 1996 19:09:37 GMT Organization: UCSF, ITS Lines: 28 Message-ID: <4nvoph$141g@itssrv1.ucsf.edu> References: NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] In humans, it appears the opposite is true. That is, that for unknown reasons, women show more chromosomal recombinants than men (especially near the centromere), and as you know, meiosis in men is generally much faster than women (male meiosis measured in days, female in tens of years). Bert Gold UCSF Americo.Bonkewitzz (Bonkewitzz@zoology.unp.ac.za) wrote: : Hi, : I am trying to find out whether the speed of Meiosis could produce non- : viables gametes or "bad gametes" (gametes with chismata-associated errors, : lack of DNA reparation, etc). There are many animal due to sperm competition : that are able to produce a tremendous amount of sperm in a short period of : time, wouldn't this lead to errors in crossing overs ? : Is it the amount of gametes to produce or the speed at which is produce what : causes non-viability or "bad gametes" ? : Has anyone got any reference or could anyone give an expert opinion ? : Thank you in advance : A. N. Bonkewitzz : bonkewitzz@zoology.unp.ac.za : bonkewit@unpsun1.cc.unp.ac.za From owner-chromosomes@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!D.UMN.EDU!rhemming From: rhemming@D.UMN.EDU (ruth hemming) Newsgroups: bionet.genome.chromosomes Subject: Faculty position Date: 22 May 1996 05:49:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605221245.HAA27278@mail.d.umn.edu> NNTP-Posting-Host: net.bio.net > > Temporary Faculty Position > > Aquatic Biology/Animal Biology/Computer Applications > >The Department of Biology at the University of Minnesota Duluth has a faculty position >at the rank of instructor available for the 1996-97 academic year with possibility of >renewal. Responsibilities of the position include teaching upper-division courses in >limnology and animal diversity, a freshman-level course in animal biology, and a course >in computer applications in biology; providing academic advisement to undergraduate >biology majors; and assisting and advising departmental faculty and staff with computer >applications. Applicants must have an M.S. degree in a biological science by the time of >appointment, part-time college teaching experience in biology, and at least one peer- >reviewed publication in aquatic biology. > >Application materials should include: > >Letter of application >Curriculum vitae that documents teaching and computer experience >Undergraduate and graduate transcripts (photocopies acceptable) >Names and telephone number of three references familiar with applicant's academic >background or teaching experience. > > Applications must be postmarked by 7 June, 1996, to: > > Donald P. Christian > Department of Biology > University of Minnesota > Duluth, MN 55812 > Telephone 218/726-7263 > FAX 218/726-8142 > email dchristi@d.umn.edu > >The University of Minnesota is an equal opportunity educator and employer. > > From owner-chromosomes@net.bio.net Wed May 22 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rcjohnsen@aol.com (Rcjohnsen) Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosomal Pet Peeve Date: 23 May 1996 13:57:54 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 5 Sender: root@newsbf02.news.aol.com Message-ID: <4o28v2$etq@newsbf02.news.aol.com> References: <4nrg0f$15lc@itssrv1.ucsf.edu> Reply-To: rcjohnsen@aol.com (Rcjohnsen) NNTP-Posting-Host: newsbf02.mail.aol.com I believe a key might be the terminology. A chromosome has a single functional centromere regardless of how many strands it carries. So Meiosis begins with strands at 2N, and after replication it goes to 4N, then at MI, it goes back to 2 N and after MII it is 1N. The number of chromosomes is 2N, 2N amd 1N respectively. From owner-chromosomes@net.bio.net Fri May 24 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!surfnet.nl!howland.reston.ans.net!EU.net!Austria.EU.net!01-newsfeed.univie.ac.at!uw.edu.pl!news.nask.pl!rose.man.poznan.pl!news.amu.edu.pl!usenet From: ameis@man.poznan.pl (Adam Meissner) Newsgroups: bionet.genome.chromosomes Subject: Slugs - how many chromosomes? Date: 24 May 1996 15:21:14 GMT Organization: Technical University of Poznan Lines: 9 Message-ID: <4o4k5a$knb@math.amu.edu.pl> NNTP-Posting-Host: 150.254.41.20 Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.6 Hello Everybody, Could anybody tell me how many chromosomes has a slug Derocros laere and Lehmonnia valentiana? Thanks in advance for any reply. Adam Meissner PS. Would you please, Cc: your reply to AMEIS@MAN.POZNAN.PL From owner-chromosomes@net.bio.net Sun May 26 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!howland.reston.ans.net!agate!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!macr3-20.welc.cam.ac.uk!user From: whc@mole.bio.cam.ac.uk (W.H. Colledge) Newsgroups: bionet.genome.chromosomes Subject: Wanted: Internet site of genome information Date: Sun, 26 May 1996 19:28:29 +0000 Organization: University of Cambridge Lines: 11 Message-ID: NNTP-Posting-Host: macr3-20.welc.cam.ac.uk Please could someone let me know of any internet sites that have information about the genomic organization of genes rather than the more usual cDNA or exon open reading frame information. A WWW site would be ideal. I am specifically interested in the genomic organisation of histone 3.3. Many thanks. -- Dr.W.H.Colledge Physiological Laboratory University of Cambridge Cambridge CB2 3EG From owner-chromosomes@net.bio.net Mon May 27 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: petersim@aol.com (Petersim) Newsgroups: bionet.genome.chromosomes Subject: DNA scientist - meet Date: 28 May 1996 12:26:25 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 18 Sender: root@newsbf02.news.aol.com Message-ID: <4of9fh$dac@newsbf02.news.aol.com> Reply-To: petersim@aol.com (Petersim) NNTP-Posting-Host: newsbf02.mail.aol.com Norman Simmons was nominated for a Nobel Prize for his work. He isolated the first samples of DNA in the 1950's. Stop in for a visit at www.tiac.net/users/perersim/ Thought your members might be interested in a video I produced about my father who in 1952 isolated the first samples of DNA. He sent these to Watson and Crick. He is now 81 years old and still as sharp as ever. In this video he describes his early work in DNA research. I thought it might be interesting to other scientists or researchers. He was nominated for the Nobel Prize for his work. See a description of this and other videos at www.tiac.net/users/perersim/ This is not a commercial message, the video is listed for $49 dollars on the web site but for members of this news group I would like to donate the video at cost plus postage. If anyone is interested in the video send me a message at petersim@aol.com. From owner-chromosomes@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!Norway.EU.net!nntp.uio.no!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!daresbury!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosomal Pet Peeve Date: Tue, 28 May 1996 16:18:45 +0100 Organization: University of Reading, U.K. Lines: 25 Message-ID: References: <4nrg0f$15lc@itssrv1.ucsf.edu> NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <4nrg0f$15lc@itssrv1.ucsf.edu> On 21 May 1996, Bert Gold wrote: > At any rate, the issue I would like your help with is this: > > How can all the textbooks continue to maintain that the number of > chromosomes during MI of Meiosis and M2 of Meiosis (until completion) > always remains at 2N? At this point they mostly admit that the > amount of DNA goes up to 4C before leptotene, but how can you > have 4C,2N? Apparently, the textbook makers think that one tetrad equals > one chromosome? At any rate, are they really counting centromeres > when they say chromosomes?? I think you're getting a bit mixed up between chromatids and chromosomes. Each chromosome has one centromere, but may have 1 or 2 chromatids, depending on the stage you're looking at. Nach McNach Jose I. de las Heras Genetics Group Botany Dept. University of Reading Reading RG6 2AU U.K. From owner-chromosomes@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: kyakerjeff@aol.com (KyakerJeff) Newsgroups: bionet.genome.chromosomes Subject: Reasearch Project I NEED HELP!!! Date: 29 May 1996 09:20:05 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 2 Sender: root@newsbf02.news.aol.com Message-ID: <4ohiu5$q8k@newsbf02.news.aol.com> References: Reply-To: kyakerjeff@aol.com (KyakerJeff) NNTP-Posting-Host: newsbf02.mail.aol.com Anyone with web or gopher sites on DNA or genetics Puleeeese Email me or post a message From owner-chromosomes@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!bofh.dot!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.genome.chromosomes Subject: Chromosome microdissection. Date: Tue, 28 May 1996 22:41:29 +0100 Organization: University of Reading, U.K. Lines: 10 Message-ID: NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I am carrying out some experiments which involve the microdissection of chromosomes, and their amplification with the DOP-PCR technique. I would like to know if there is anybody here with expertise in that area so he/she can advise me about a couple of points. J.I. de las Heras Botany Dept. University of Reading Reading UK From owner-chromosomes@net.bio.net Wed May 29 23:00:00 1996 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.genome.chromosomes Subject: Genomic labelling in-situ of plant DNA Date: Thu, 30 May 1996 22:08:09 +0100 Organization: University of Reading, U.K. Lines: 21 Message-ID: NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I am working with wheat. I want to intercross two lines, but I want to keep track, in the very early embryo and endosperm development, of the parental chromosomes. I thought of introducing tritiated thimidine into the spikes, previous to meiosis. Any other way of labelling, not involving tritium? Any thoughts as to what would be the best way to introduce the labelled compound into the plant? I thought of putting a fine thread through the stem, wetted in the labelling solution, and let the transpiration flow carry the label to the pollen mother cells, and to the eggs. Any better ways? Thank you for any help Nacho de las Heras Botany Dept. University of Reading Reading UK From owner-chromosomes@net.bio.net Thu May 30 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!netcom.net.uk!ix.netcom.com!news From: hk-miami@ix.netcom.com(HK) Newsgroups: bionet.genome.chromosomes Subject: Re: Reasearch Project I NEED HELP!!! Date: 31 May 1996 22:55:24 GMT Organization: Netcom Lines: 11 Message-ID: <4ontcs$pkl@dfw-ixnews4.ix.netcom.com> References: <4ohiu5$q8k@newsbf02.news.aol.com> NNTP-Posting-Host: mia-fl2-11.ix.netcom.com X-NETCOM-Date: Fri May 31 5:55:24 PM CDT 1996 In <4ohiu5$q8k@newsbf02.news.aol.com> kyakerjeff@aol.com (KyakerJeff) writes: > >Anyone with web or gopher sites on DNA or genetics Puleeeese Email me or >post a message ======================================================================= ===Why don't you just do a search? Then, you can select the sites that are of interest to you. To list all the sites concerning DNA and genetics would be a huge task. HK From owner-chromosomes@net.bio.net Fri May 31 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!usenet1.news.uk.psi.net!uknet!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!nntp0.brunel.ac.uk!strath-cs!st-and!Aberdeen!usenet From: "A. Hamali" Newsgroups: bionet.genome.chromosomes Subject: Re: definition of "Genome" Date: 30 May 1996 14:37:28 GMT Organization: University of Aberdeen, Scotland Lines: 3 Message-ID: <4okbr8$saq@info.abdn.ac.uk> References: <318F7A86.6890@student.uq.edu.au> NNTP-Posting-Host: fhdpc2.ugrad.abdn.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) To: Andrea,Crampton HI I will be glad if you could the forward the answer if you get it thanks