From owner-chromosomes@net.bio.net Thu Jul 04 23:00:00 1996 Path: biosci!biosci!not-for-mail From: DSF <76042.532@CompuServe.COM> Newsgroups: bionet.genome.chromosomes,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.methds-reagnts Subject: QIAGEN MINIPREP ROBOT Date: 4 Jul 1996 23:40:21 -0700 Organization: Cadus Lines: 4 Sender: biohelp@net.bio.net Distribution: world Message-ID: <4qu5he$c0k$1@mhafc.production.compuserve.com> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.genome.chromosomes:1251 bionet.molbio.embldatabank:656 bionet.molbio.genbank:2320 bionet.molbio.methds-reagnts:46536 Has anyone had any experience with the qiagen miniprep robots especially with regards to sequencing on the abi sequencers (373 and 377) please reply by e-mail. thank you From owner-chromosomes@net.bio.net Thu Jul 04 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news-res.gsl.net!news.gsl.net!hunter.premier.net!newsfeed.internetmci.com!swrinde!tank.news.pipex.net!pipex!oleane!in2p3.fr!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!cs.tu-berlin.de!uni-erlangen.de!lrz-muenchen.de!ipp-garching.mpg.de!wanze!souza From: souza@wanze (Augustine Souza) Newsgroups: bionet.genome.chromosomes,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.methds-reagnts Subject: Re: QIAGEN MINIPREP ROBOT Followup-To: bionet.genome.chromosomes,bionet.molbio.embldatabank,bionet.molbio.genbank,bionet.molbio.methds-reagnts Date: 5 Jul 1996 14:22:32 GMT Organization: Rechenzentrum der Max-Planck-Gesellschaft in Garching Lines: 9 Distribution: world Message-ID: <4rj8f8$6eoe@sat.ipp-garching.mpg.de> References: <4qu5he$c0k$1@mhafc.production.compuserve.com> NNTP-Posting-Host: wanze.biochem.mpg.de X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.genome.chromosomes:1252 bionet.molbio.embldatabank:657 bionet.molbio.genbank:2321 bionet.molbio.methds-reagnts:46552 DSF (76042.532@CompuServe.COM) wrote: : Has anyone had any experience with the qiagen miniprep robots : especially with regards to sequencing on the abi sequencers (373 : and 377) please reply by e-mail. : thank you Why don't you get a postdoc? Gus From owner-chromosomes@net.bio.net Wed Jul 10 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news-stk-200.sprintlink.net!news.vol.it!peroni.ita.tip.net!news.flashnet.it!usenet From: Donatella Mastrangelo Newsgroups: bionet.genome.chromosomes Subject: Help for Giuseppe Date: Thu, 11 Jul 1996 00:50:28 +0000 Organization: Flashnet Lines: 19 Message-ID: <31E44FD4.39D6@flashnet.it> Reply-To: donmas@flashnet.it NNTP-Posting-Host: ppp-043.flashnet.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) I really don't know if this is the right newsgroup, but I try anyway to tell you about Giuseppe. He is a young boy (9 years old) affected by GM1 Gangliosidosis. This kind of illness will be letal. It is called Sandoff Desease. Is there anybody who would be able to tell us information and/or indications of specialized person who could take care of him. There are only few cases in the world and that is the reason why it is so difficult to find help. Please, let us have as much as possible to help him. We are calling from Italy. Thank you so much! Donatella Mastrangelo e-mail: jadeye@flashnet.it From owner-chromosomes@net.bio.net Fri Jul 12 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!newsreader.sprintlink.net!news.sprintlink.net!news-dc-10.sprintlink.net!sun.quiknet.com!news From: zanedick@mail2.quiknet.com (Dan Mc Alpine) Newsgroups: bionet.genome.chromosomes Subject: People! lets get some good posting or or or I'll Date: 11 Jul 1996 22:51:33 GMT Organization: Quiknet Information Services. (916) 773-0917. http://www.quiknet.com Lines: 6 Message-ID: <4s40hl$hgu@sun.quiknet.com> Reply-To: zanedick@mail2.quiknet.com NNTP-Posting-Host: fo-101.quiknet.com Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Newsreader: WinVN 0.92.6+ I'll write one for you!, Sincerely, Dan From owner-chromosomes@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!spool.mu.edu!uwm.edu!news-res.gsl.net!news.gsl.net!swrinde!elroy.jpl.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!news.fibr.net!nntp.primenet.com!news.primenet.com!clark From: clark@primenet.com Newsgroups: bionet.genome.chromosomes Subject: n Date: 15 Jul 1996 20:25:01 -0700 Organization: Primenet (602)416-7000 Lines: 22 Message-ID: <4sf22d$q7c@nnrp1.news.primenet.com> X-Posted-By: clark@usr07.primenet.com Oh, I hope I am doing this right! Anyway, you want a posting about genetic disorders? My son is 20 months old and has Opitz-G Syndrome, which is pretty rare from what I was told. Opitz-G is also sometimes called Midline Defects because everything affected is in the midline. He was born with Hypertelorism (Telecanthus), Cleft Lip, a cleft in his gum line (part was missing), a cleft in his larynx, and Hypospadius, and we believe Hypotonia even though that has not been offically diagnosed. I have had a problem finding anyone on the net who has any info with this. I actually have done quite a bit of reasearch into Opitz-G Syndrome, but have never met anyone else who has it, and would be interested in talking to anyone who has this in thier family, or would like to talk about this. Also if anyone has any suggestions about places to look on the net for newsgroups or whatever related to birth defects or syndromes, please email and tell me. Thank you! Charotte clark@primenet.com -- ------------------------------------------------------------------------------- clark __ __ ____ ___ ___ ____ clark@primenet.com /__)/__) / / / / /_ /\ / /_ / / / \ / / / / /__ / \/ /___ /------------------------------------------------------------------------------- From owner-chromosomes@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!hunter.premier.net!newsfeed.internetmci.com!csn!nntp-xfer-1.csn.net!magnus.acs.ohio-state.edu!ts24-2.homenet.ohio-state.edu!user From: snyder.9@osu.edu (Pam Snyder) Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Subject: Re: looking for information about the APCR genetic disease Date: Sun, 14 Jul 1996 13:33:05 -0500 Organization: Just me Lines: 55 Message-ID: References: <31EBA253.C83@resulb.ulb.ac.be> NNTP-Posting-Host: ts24-2.homenet.ohio-state.edu Xref: biosci bionet.general:22780 bionet.info-theory:4182 bionet.molbio.gene-linkage:1118 bionet.genome.chromosomes:1261 In article <31EBA253.C83@resulb.ulb.ac.be>, Stephane Corteel wrote: > A few weeks ago, we were informed that my wife (28 years old) is affected by a > genetic disease called APCR. > > The only information we have about this disease are : > > - it is a genetic disease apparently due to the mutation of gene > > - it has been recently discovered (2 or 3 years ago) > > - it is related to blood coagulation > > - The effects are increased if the patient eats food containing a high > concentration in vitamin K > > - Due to this disease, my wife has alrealy made 2 pulmonary embolisms > in 1 year. > > Can someon provide us with more information about this problem, references > in scientific litterature, known treatments, aso... > > Thank you for all your answers. > Stephane Corteel, scorteel@resulb.ulb.ac.be I think you are referring to activated Protein C resistance, which is caused by a mutation in the gene encoding for the factor V molecule. This molecule greatly increases the rate of clot formation and is regulated by another protein (activated protein C). APC regulates factor V by cleaving it to reduce the rate of clot formation. If Factor V cannot be appropriately cleaved, clot formation can result. The Mutant Factor V is called Factor V Leiden. The theory is it takes more than just one copy of Factor V Leiden to cause problems (since 5% of the Factor V Leiden heterozygotes do not have clotting problems). The second "hit" could be genetic (as in another clotting factor) or environmental. (Oral contraceptives are a risk factor, as is any kind of trauma). There is a direct molecular test available for this mutation. I assume your wife has been diagnosed molecularly. The following paper defined the mutation, Nature 369:64-7 (1994) There are hundreds of papers out there on the subject right now. A medline search yields, I believe, greater than 300. Search for Factor V, Mutation This newsgroup is subcribed by several labs who test for the assay as well, including the one I work in at the Ohio State University Hospitals. From owner-chromosomes@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!netnews.worldnet.att.net!ix.netcom.com!news From: jjberman@ix.netcom.com(Jules Berman ) Newsgroups: bionet.genome.chromosomes Subject: Announcement: Disease search engine Date: 16 Jul 1996 16:14:05 GMT Organization: Netcom Lines: 25 Message-ID: <4sgf4d$j1q@dfw-ixnews4.ix.netcom.com> NNTP-Posting-Host: bal-md8-11.ix.netcom.com X-NETCOM-Date: Tue Jul 16 11:14:05 AM CDT 1996 Pathology Informatics, Inc., invites health professionals to visit our new web site: http://www.pathinfo.com/ This is the location of our Lightning Hypertext of Disease search engine, that performs simple Boolean searches on a database composed of more than 21,000 information packets related to disease biology and related issues, inluding extensive treatment of cytogenetics and chromosome markers/mapping and tumor biology. All searches are free, and, at this time, there is no limit to the number of permitted searches. Some of the fields covered by the Lightning Hypertext are ignored by traditional paper texts, and include: statistics, epidemiology, incidence data, molecular biology and internet issues. Most of the information is geared to health professionals. Therefore, OUR SEARCH ENGINE SHOULD NOT BE USED BY LAY PERSONS INTERESTED IN SELF-DIAGNOSIS OR SELF-TREATMENT. Queries and comments can be e-mailed to Jules Berman at: jjberman@ix.netcom.com From owner-chromosomes@net.bio.net Mon Jul 15 23:00:00 1996 Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!uchinews!woodlawn!aekentsi From: aekentsi@woodlawn.uchicago.edu (alex emil kentsis) Subject: Re: looking for information about the APCR genetic disease X-Nntp-Posting-Host: woodlawn.uchicago.edu Message-ID: Followup-To: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago X-Newsreader: TIN [version 1.2 PL2] References: <31EBA253.C83@resulb.ulb.ac.be> Date: Tue, 16 Jul 1996 14:23:23 GMT Lines: 28 Xref: biosci bionet.general:22776 bionet.info-theory:4181 bionet.molbio.gene-linkage:1117 bionet.genome.chromosomes:1259 what does APCR stand for? i did a medline search for "apcr" and it revealed nothing of relevance. Stephane Corteel (scorteel@resulb.ulb.ac.be) wrote: : A few weeks ago, we were informed that my wife (28 years old) is affected by a : genetic disease called APCR. : The only information we have about this disease are : : - it is a genetic disease apparently due to the mutation of gene : - it has been recently discovered (2 or 3 years ago) : - it is related to blood coagulation : - The effects are increased if the patient eats food containing a high : concentration in vitamin K : - Due to this disease, my wife has alrealy made 2 pulmonary embolisms : in 1 year. : Can someon provide us with more information about this problem, references : in scientific litterature, known treatments, aso... : Thank you for all your answers. : Stephane Corteel, scorteel@resulb.ulb.ac.be From owner-chromosomes@net.bio.net Mon Jul 15 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!oleane!jussieu.fr!news.belnet.be!news.vub.ac.be!usenet From: Stephane Corteel Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Subject: looking for information about the APCR genetic disease Date: Tue, 16 Jul 1996 16:08:19 +0200 Organization: ULB Lines: 22 Message-ID: <31EBA253.C83@resulb.ulb.ac.be> NNTP-Posting-Host: llpc.ulb.ac.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01Gold (Win95; I) CC: scorteel@resulb.ulb.ac.be, llibert@ulb.ac.be Xref: biosci bionet.general:22775 bionet.info-theory:4180 bionet.molbio.gene-linkage:1116 bionet.genome.chromosomes:1258 A few weeks ago, we were informed that my wife (28 years old) is affected by a genetic disease called APCR. The only information we have about this disease are : - it is a genetic disease apparently due to the mutation of gene - it has been recently discovered (2 or 3 years ago) - it is related to blood coagulation - The effects are increased if the patient eats food containing a high concentration in vitamin K - Due to this disease, my wife has alrealy made 2 pulmonary embolisms in 1 year. Can someon provide us with more information about this problem, references in scientific litterature, known treatments, aso... Thank you for all your answers. Stephane Corteel, scorteel@resulb.ulb.ac.be From owner-chromosomes@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!sgigate.sgi.com!spool.mu.edu!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Something living on colchicine! Date: Wed, 17 Jul 1996 20:26:46 +0100 Organization: University of Reading, U.K. Lines: 16 Message-ID: NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.molbio.methds-reagnts:47022 bionet.genome.chromosomes:1265 Hello all, I just took a flask of colchicine stock solution (0.1M, or about 4%) that had been kept at 4 degrees for about a year... and I found "something" living on the bottom! Colchicine is a strong mutagen. The concentration used to arrest cellular development at metaphase is a hundred times lower than that in the flask. I'm amazed some fungi can grow in those conditions! Nacho de las Heras Botany Department University of Reading Reading RG6 2AS UK From owner-chromosomes@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!oleane!jussieu.fr!uvsq.fr!Newsmaster From: Sid-Ahmed BOUKABARA Newsgroups: bionet.genome.chromosomes Subject: <>> Date: 17 Jul 1996 08:58:16 GMT Organization: Universite de Versailles/St Quentin en Yvelines - France Lines: 26 Message-ID: <4si9v8$sb9@soleil.uvsq.fr> NNTP-Posting-Host: balsa.cetp.ipsl.fr Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 4.1.3 sun4m) X-URL: file:/users/obt/boukabar/y bonjour, Je ne suis pas etudiant en medecine et encore moins medecin, je veux simplement vous demander de me renseigner pour une personne qui a un germe dans sa gorge qui s'appelle l'haemophilus parain fluenz Voici ses questions: 1- Mes manifestations chimiques sont des angines rouges a repetition, y'a -t-il d'autres manifestations pour l'haemophilus parainfluenz? Peuvent elles partir sans traitement ou faut il les traiter? 2- quel est l'origine de l'haemophilus parainfluenz? 3- comment se traite ce germe et combien dure le traitement? 4- y'a-t-il possibilite de recidive? 5- Comment contacte-t-on l'haemophilus parainfluenz et pourquoi (les causes)? En vous remerciant infinimemt pour votre reponse. Merci beaucoup. ps:pouvez vous m'envoyer une copie sur ma boite aux e-mails (car je ne suis pas souvent sur Internet) From owner-chromosomes@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!oleane!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!hole.news.pipex.net!pipex!duke.telepac.pt!news.telepac.pt!usenet From: Raul Osorio Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Subject: Re: looking for information about the APCR genetic disease Date: Wed, 17 Jul 1996 08:56:01 +0200 Organization: TELEPAC, SA Lines: 40 Message-ID: <31EC8E81.4A7A@mail.telepac.pt> References: <31EBA253.C83@resulb.ulb.ac.be> NNTP-Posting-Host: sh1_p1.telepac.pt Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b5 (Win95; I) To: Stephane Corteel Xref: biosci bionet.general:22791 bionet.info-theory:4184 bionet.molbio.gene-linkage:1121 bionet.genome.chromosomes:1263 Stephane Corteel wrote: > > A few weeks ago, we were informed that my wife (28 years old) is affected by a > genetic disease called APCR. > > The only information we have about this disease are : > > - it is a genetic disease apparently due to the mutation of gene > > - it has been recently discovered (2 or 3 years ago) > > - it is related to blood coagulation > > - The effects are increased if the patient eats food containing a high > concentration in vitamin K > > - Due to this disease, my wife has alrealy made 2 pulmonary embolisms > in 1 year. > > Can someon provide us with more information about this problem, references > in scientific litterature, known treatments, aso... > > Thank you for all your answers. > Stephane Corteel, scorteel@resulb.ulb.ac.be Although I don't know the disease, (maybe if you explain the acronym APCR...), it seems to be somehow related with an excess of blood clotting factors or their activity, probably those wich are dependent on vitamin K for their synthesis. One possible course of treatment could be the use of anticoagulants, in order to reduce the clotting activity of your wive's blood to normal levels. Anyway, since I work on a genetics institute (although not on the field of genetics), I'll try and ask my coleagues about the disease, and I'll come back to you with anything I find out. Raul Osorio, MD From owner-chromosomes@net.bio.net Tue Jul 16 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!oleane!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!hole.news.pipex.net!pipex!duke.telepac.pt!news.telepac.pt!usenet From: Raul Osorio Newsgroups: bionet.general,bionet.info-theory,bionet.molbio.gene-linkage,bionet.genome.chromosomes,fr.bio.general Subject: Re: looking for information about the APCR genetic disease Date: Wed, 17 Jul 1996 08:52:12 +0200 Organization: TELEPAC, SA Lines: 40 Message-ID: <31EC8D9C.327@mail.telepac.pt> References: <31EBA253.C83@resulb.ulb.ac.be> NNTP-Posting-Host: sh1_p1.telepac.pt Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b5 (Win95; I) To: Stephane Corteel Xref: biosci bionet.general:22790 bionet.info-theory:4183 bionet.molbio.gene-linkage:1120 bionet.genome.chromosomes:1262 Stephane Corteel wrote: > > A few weeks ago, we were informed that my wife (28 years old) is affected by a > genetic disease called APCR. > > The only information we have about this disease are : > > - it is a genetic disease apparently due to the mutation of gene > > - it has been recently discovered (2 or 3 years ago) > > - it is related to blood coagulation > > - The effects are increased if the patient eats food containing a high > concentration in vitamin K > > - Due to this disease, my wife has alrealy made 2 pulmonary embolisms > in 1 year. > > Can someon provide us with more information about this problem, references > in scientific litterature, known treatments, aso... > > Thank you for all your answers. > Stephane Corteel, scorteel@resulb.ulb.ac.be Although I don't know the disease, (maybe if you explain the acronym APCR...), it seems to be somehow related with an excess of blood clotting factors or their activity, probably those wich are dependent on vitamin K for their synthesis. One possible course of treatment could be the use of anticoagulants, in order to reduce the clotting activity of your wive's blood to normal levels. Anyway, since I work on a genetics institute (although not on the field of genetics), I'll try and ask my coleagues about the disease, and I'll come back to you with anything I find out. Raul Osorio, MD From owner-chromosomes@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!oleane!in2p3.fr!swidir.switch.ch!scsing.switch.ch!elna.ethz.ch!usenet From: Michael Kertesz Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Thu, 18 Jul 1996 20:11:08 +0200 Organization: Microbiology ETHZ Lines: 31 Message-ID: <31EE7E3C.1148@micro.biol.ethz.ch> References: NNTP-Posting-Host: b22-pro486-1.ethz.ch Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Xref: biosci bionet.molbio.methds-reagnts:47082 bionet.genome.chromosomes:1268 Quick!! Characterize and publish!! Chromosome Terror wrote: > > Hello all, > > I just took a flask of colchicine stock solution (0.1M, or about 4%) that > had been kept at 4 degrees for about a year... and I found "something" > living on the bottom! > Colchicine is a strong mutagen. The concentration used to arrest cellular > development at metaphase is a hundred times lower than that in the flask. > I'm amazed some fungi can grow in those conditions! > > Nacho de las Heras > Botany Department > University of Reading > Reading RG6 2AS > UK -- Michael Kertesz ETH-Microbiology, ETH-Zentrum/LFV, CH-8092 Zurich, Switzerland tel: +41-1-632 3357 fax: +41-1-632 1148 e-mail: kertesz@micro.biol.ethz.ch From owner-chromosomes@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!oleane!jussieu.fr!univ-lyon1.fr!cri.ens-lyon.fr!news From: Olivier Gandrillon Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Thu, 18 Jul 1996 10:33:23 +0100 Organization: Ecole Normale Superieure de Lyon Lines: 20 Message-ID: <31EE04E3.16FD@cri.ens-lyon.fr> References: Reply-To: Olivier.Gandrillon@ens-lyon.fr NNTP-Posting-Host: mac-lr5-143b.ens-lyon.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) To: Chromosome Terror Xref: biosci bionet.molbio.methds-reagnts:47054 bionet.genome.chromosomes:1267 Better get rid of it before it invades us! Olivier Chromosome Terror wrote: > > Hello all, > > I just took a flask of colchicine stock solution (0.1M, or about 4%) that > had been kept at 4 degrees for about a year... and I found "something" > living on the bottom! > Colchicine is a strong mutagen. The concentration used to arrest cellular > development at metaphase is a hundred times lower than that in the flask. > I'm amazed some fungi can grow in those conditions! > > Nacho de las Heras > Botany Department > University of Reading > Reading RG6 2AS > UK From owner-chromosomes@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!daresbury!is.bbsrc.ac.uk!news From: Andrei Popov Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Thu, 18 Jul 1996 22:37:59 +0000 Organization: BBSRC Biotechnology and Biological Sciences Research Council Lines: 30 Message-ID: <31EEBCC7.2644@bbsrc.ac.uk> References: NNTP-Posting-Host: pc0743.iapc.bbsrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Xref: biosci bionet.molbio.methds-reagnts:47098 bionet.genome.chromosomes:1270 Chromosome Terror wrote: > > Hello all, > > I just took a flask of colchicine stock solution (0.1M, or about 4%) that > had been kept at 4 degrees for about a year... and I found "something" > living on the bottom! > Colchicine is a strong mutagen. The concentration used to arrest cellular > development at metaphase is a hundred times lower than that in the flask. > I'm amazed some fungi can grow in those conditions! > > Nacho de las Heras > Botany Department > University of Reading > Reading RG6 2AS > UK I had a similar observation. But in my case the bugs were bacteria. Andrei From owner-chromosomes@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!info.ucla.edu!news.bc.net!unixg.ubc.ca!van-bc!n1van.istar!van.istar!west.istar!ott.istar!istar.net!news.nstn.ca!news From: Valerie Mansour Newsgroups: bionet.genome.chromosomes Subject: genetic research Date: Thu, 18 Jul 1996 17:54:16 -0300 Organization: Nova Scotia Technology Network Lines: 7 Message-ID: <31EEA478.5F2D@cochran.com> NNTP-Posting-Host: mac20.cochran.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; 68K) Hello: I'm working on a tv series about the Internet and I'm looking for a parent, in Canada, of a child with a genetic disorder who uses the net for genetic research and is also a member of a support group. Thanks. From owner-chromosomes@net.bio.net Wed Jul 17 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!oleane!jussieu.fr!rain.fr!news.sprintlink.net!news-dc-9.sprintlink.net!news.up.net!news From: John Gatiss Newsgroups: bionet.genome.chromosomes Subject: Colleges Date: Wed, 17 Jul 1996 23:31:18 -0400 Organization: up.net Lines: 3 Message-ID: <31EDB006.E0C@up.net> NNTP-Posting-Host: mun1-175.up.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02Gold (Win95; I) I'd be interested in hearing the viewpoints of some biologists regarding a good school to study biology. I hope to get involved in the genetics field, so is there any college that is particularlly renound in this area? (Not necessarily your alma mater ;-) From owner-chromosomes@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!news2.acs.oakland.edu!newsfeed.concentric.net!news-master!news From: "M. Plewinska" Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Fri, 19 Jul 1996 04:41:52 -0400 Organization: Miami, FL, USA Lines: 8 Message-ID: <31EF4A50.4E4B@concentric.net> References: NNTP-Posting-Host: cnc020038.concentric.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win95; U) Xref: biosci bionet.molbio.methds-reagnts:47105 bionet.genome.chromosomes:1271 I had comething sprout in my DEPC water (the stuff you use to inhibit RNAses). Just goes to show: there is a bug for every situation out there. -- - Magda Plewinska Miami, FL, USA University of Miami Genetics From owner-chromosomes@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!agate!dog.ee.lbl.gov!news.cs.utah.edu!news.cc.utah.edu!howard!rafael From: Rafael Maldonado Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Thu, 18 Jul 1996 13:40:01 -0600 Organization: University of Utah Computer Center Lines: 39 Message-ID: References: NNTP-Posting-Host: howard.genetics.utah.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: rafael@howard In-Reply-To: Xref: biosci bionet.molbio.methds-reagnts:47127 bionet.genome.chromosomes:1272 On Wed, 17 Jul 1996, Chromosome Terror wrote: > > Hello all, > > I just took a flask of colchicine stock solution (0.1M, or about 4%) that > had been kept at 4 degrees for about a year... and I found "something" > living on the bottom! > Colchicine is a strong mutagen. The concentration used to arrest cellular > development at metaphase is a hundred times lower than that in the flask. > I'm amazed some fungi can grow in those conditions! Are you sure that they are fungi? Bacteria are pretty resistant to colchicine. Or may be those fungi cannot permeate colchicine well. Another possibility is that colchicine was inactivated after a year. Or may be you have a contamination of Fusarium colchicinofilus Mal., a fungus that need colchicin to grow when it is not a symbiont of the giant moth of the Amazonas. Lo que no cabe duda es que eres un guarro. Rafa ___________________________________________________________________ | Rafael Maldonado | 'No te creas todo room 6160 Eccles Institute of Human Genetics | lo que leas en Department of Human Genetics | internet.' University of Utah | Salt Lake City, Utah 84112. USA. | "Don't believe Rafael.Maldonado@genetics.utah.edu | everything you read Rafael@howard.genetics.utah.edu | in internet." Tel: 801-581-4429 | Fax: 801-585-3910 | From owner-chromosomes@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!chi-news.cic.net!cs.utexas.edu!cs.utexas.edu!swrinde!cssun.mathcs.emory.edu!cs.utk.edu!gaia.ns.utk.edu!tcooper2.utmem.edu!user From: vvsvetlov@utmem1.utmem.edu (killer yeast) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Fri, 19 Jul 1996 13:32:54 +0800 Organization: Lord Byron Memorial STD Distribution Centre Lines: 18 Message-ID: References: NNTP-Posting-Host: tcooper2.utmem.edu Xref: biosci bionet.molbio.methds-reagnts:47138 bionet.genome.chromosomes:1273 In article , Chromosome Terror wrote: :> I just took a flask of colchicine stock solution (0.1M, or about 4%) that :> had been kept at 4 degrees for about a year... and I found "something" :> living on the bottom! :> Colchicine is a strong mutagen. The concentration used to arrest cellular :> development at metaphase is a hundred times lower than that in the flask. :> I'm amazed some fungi can grow in those conditions! There are colchicine and colcemide resistant strains of yeast around, the same might be true for other fungi (some lysine mutants in tubulin for example). To share a horror story - something is growing at the bottom of John's 1% cycloheximide in the cold room! Looks like them filamentous fungi only more sinister... Check in the cold room if you don't see me for few days. Me wife's phone number is above the table. Regards, Vlad From owner-chromosomes@net.bio.net Thu Jul 18 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!chi-news.cic.net!nntp.coast.net!sdd.hp.com!spool.mu.edu!usenet.eel.ufl.edu!tank.news.pipex.net!pipex!oleane!jussieu.fr!math.ohio-state.edu!news.physics.uiowa.edu!newsrelay.iastate.edu!news.iastate.edu!macgrant.agron.iastate.edu!user From: dgrant@iastate.edu (David Grant) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Fri, 19 Jul 1996 15:28:21 -0500 Organization: Iowa State University Lines: 1 Message-ID: References: NNTP-Posting-Host: macgrant.agron.iastate.edu Xref: biosci bionet.molbio.methds-reagnts:47143 bionet.genome.chromosomes:1274 I once found something (probably bacteria) growing in my 20% SDS in ddH2O From owner-chromosomes@net.bio.net Sat Jul 20 23:00:00 1996 Path: biosci!nntp.ucr.edu!ihnp4.ucsd.edu!munnari.OZ.AU!harbinger.cc.monash.edu.au!nntp.coast.net!sgigate.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!news.campus.mci.net!news.uky.edu!usenet From: "Michael W. Thompson" Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: 21 Jul 1996 21:18:20 GMT Organization: University of Kentucky Lines: 4 Message-ID: <4su6qs$a09@service3.uky.edu> References: NNTP-Posting-Host: 128.163.70.124 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; PPC) X-URL: news:dgrant-1907961528210001@macgrant.agron.iastate.edu Xref: biosci bionet.molbio.methds-reagnts:47168 bionet.genome.chromosomes:1275 I once found something growing in my 4% Sodium Desoxycholate...now don't tell me that's not one NASTY fellow... From owner-chromosomes@net.bio.net Sun Jul 21 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!uwm.edu!news-res.gsl.net!news.gsl.net!swrinde!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!serra.unipi.it!sirio.cineca.it!galileo.polito.it!usenet From: Matteo D'Ambrosio Newsgroups: bionet.genome.chromosomes Subject: HELP: SMA 11 (Spine Muscular Atrophy) Date: Mon, 22 Jul 1996 07:37:21 -0700 Organization: CSELT Lines: 16 Message-ID: <31F39221.5890@cselt.stet.it> NNTP-Posting-Host: drogo.cselt.stet.it Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Roberto is a little child (2 years old) living in Italy. He is affected by a serious genetic desease, which is itemized as: SMA 11 (Spine Muscular Atrophy) with deletion of the exo. no. 7 and 8 gene SMA. Roberto is still hoping to meet someone who can help him. We are looking for specialists in this area, anywhere in the world, who could take care of him. Any kind of information and/or indications are welcome. Thank you! (Please send messages to : matteo.dambrosio@cselt.stet.it) From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!klenchin From: klenchin@facstaff.wisc.edu (Dima Klenchin) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Wed, 24 Jul 96 03:11:29 GMT Organization: UW-Madison Lines: 11 Message-ID: <4t40p4$2ll8@news.doit.wisc.edu> References: <4su6qs$a09@service3.uky.edu> NNTP-Posting-Host: f182-145.net.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 8bit X-Newsreader: News Xpress 2.0 Beta #2 Xref: biosci bionet.molbio.methds-reagnts:47242 bionet.genome.chromosomes:1277 In article <4su6qs$a09@service3.uky.edu>, "Michael W. Thompson" wrote: # I once found something growing in my 4% # Sodium Desoxycholate...now don't tell me #that's not one NASTY fellow... I have found some filamentous crap growing in 20% sodium azide!!! I would NEVER believe it is possible if I were not 100% sure it was not present at a time of preparation (many month ago). - Dima From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!hunter.premier.net!news.cais.net!netaxs.com!wyndmoor1-23.slip.netaxs.com!user From: provider@netaxs.com (David W. Bradley, Ph.D.) Newsgroups: bionet.genome.chromosomes Subject: Chromosome Group for Open Peer Review Date: Wed, 24 Jul 1996 13:05:43 -0400 Organization: Information Providers, Inc. Lines: 7 Message-ID: NNTP-Posting-Host: wyndmoor1-23.slip.netaxs.com The World Journal Club is forming an Chromosome Group for publishing and reviewing scientific data on the internet. Please let us know if you would like to participate. See http://www.journalclub.com/artfrnt.html for an example publication. From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!uwm.edu!news-res.gsl.net!news.gsl.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!strath-cs!st-and!Aberdeen!usenet From: gen159@abdn.ac.uk (David D Morgan) Newsgroups: bionet.genome.chromosomes Subject: genome projects worldwide Date: Tue, 23 Jul 1996 15:22:56 GMT Organization: Molecular & Cell Biology Lines: 17 Message-ID: <4t5jgp$2eh@info.abdn.ac.uk> Reply-To: gen159@abdn.ac.uk NNTP-Posting-Host: sysb.abdn.ac.uk X-Newsreader: Forte Free Agent v0.55 Does anyone have any info about the whereabouts of information regarding the status of the various genome projects that are going on ? Thanks Dave Morgan ******************************************************************************** * David D Morgan | **. .***. .***. .***. .** * * Dept Molecular & Cell | | | * * | | | * | | | * * | | | * | | * * Biology | | | | * * | | | * * | | | * * | | | * * | * * University of Aberdeen | * | | | * | | | * * | | | * | | | * * * * Aberdeen, SCOTLAND | '***' '***' '***' '***' * * Tel: 01224 273105 | * * Fax: 01224 273144 | WWW: http://www.abdn.ac.uk/~gen159/ * * Email: gen159@abdn.ac.uk | * ******************************************************************************** From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!dale.ucdavis.edu!not-for-mail From: szwrobel@dale.ucdavis.edu (Russell Wrobel) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Followup-To: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Date: 24 Jul 1996 04:34:21 GMT Organization: University of California, Davis Lines: 20 Message-ID: <4t494d$576@mark.ucdavis.edu> References: <4su6qs$a09@service3.uky.edu> <4t40p4$2ll8@news.doit.wisc.edu> NNTP-Posting-Host: dale.ucdavis.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Xref: biosci bionet.molbio.methds-reagnts:47245 bionet.genome.chromosomes:1278 Dima Klenchin (klenchin@facstaff.wisc.edu) wrote: : In article <4su6qs$a09@service3.uky.edu>, "Michael W. Thompson" wrote: : # I once found something growing in my 4% : # Sodium Desoxycholate...now don't tell me : #that's not one NASTY fellow... : : I have found some filamentous crap growing in 20% sodium azide!!! : I would NEVER believe it is possible if I were not 100% sure it was not : present at a time of preparation (many month ago). : : - Dima : Did anyone ever throw these mysterious super "organism" under the microscope to actually see if they were cellular? I to once was filamentous stuff in my azide stock but after a brief look in the microscope I found that it was definately not cellular but was most likely some kind of precipitate. Russell From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!agate!spool.mu.edu!uwm.edu!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!oleane!jussieu.fr!infobiogen.fr!sansgene.genethon.fr!not-for-mail From: bernot@sansgene.genethon.fr (Alain Bernot) Newsgroups: bionet.genome.chromosomes Subject: Re: genome projects worldwide Date: 24 Jul 1996 20:54:16 +0200 Organization: Genethon -- Human Genome Research Centre Lines: 14 Message-ID: <4t5rgo$l7e@sansgene.genethon.fr> References: <4t5jgp$2eh@info.abdn.ac.uk> NNTP-Posting-Host: sansgene.genethon.fr To: gen159@abdn.ac.uk the server of the Sanger Center (which is sequencing the worm C. elegans and parts of the human genome) proposes many links to other genome centers involved in various genome project http://www.sanger.ac.uk see also: genethon : http://www.genethon.fr whitehead institute : http://www-genome.wi.mit.edu WashU : http://ibc.wustl.edu TIGR : http://www.tigr.org CHLC : http://www.cshl.org etc.... From owner-chromosomes@net.bio.net Tue Jul 23 23:00:00 1996 Path: biosci!SKI.MSKCC.ORG!a-lustig From: a-lustig@SKI.MSKCC.ORG (Dr. Arthur Lustig) Newsgroups: bionet.genome.chromosomes Subject: postdoctoral positions Date: 24 Jul 1996 14:27:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITIONS AVAILABLE TO STUDY TELOMERE SIZE REGULATION AND TELOMERIC SILENCING IN SACCHAROMYCES CEREVISIAE. I am in the process of moving my lab to Tulane University Medical Center in New Orleans and am seeking qualified postdoctoral researchers to study one of two problems: a) the role of the telomere-binding protein RAP1 in telomere size control and telomeric silencing (see Lustig et al. MCB 16: 2483-2495, 1996); and b) the characterization of a novel process, called telomeric rapid deletion (Li and Lustig, Genes Dev. 10:1310-1326, 1996), that appears to combine elements of intrachromatid recombination and pairing of non-homologous telomeres to regulate telomere size. Interested applicants can respond by e-mail or at this address until August 9. After that time, applicants can contact me at alustig@mailhost.tcs. tulane.edu. or at the following address. Department of Biochemistry SL43 Tulane University Medical Center 1430 Tulane Avenue New Orleans, LA 70112. Arthur J. Lustig, Ph.D Head Laboratory of Chromosome Structure Memorial Sloan-Kettering Cancer Center 1275 York Avenue New York, NY 10021 212-639-7986 (phone) 212-639-2861 (fax) a-lustig@ski.mskcc.org (e-mail) From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Rifat Hamoudi Newsgroups: bionet.genome.chromosomes Subject: Making cells loose their DNA material Date: 25 Jul 1996 17:41:11 +0100 Lines: 9 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4t8837$qoj@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: IMSP Original-To: biochrom@dl.ac.uk Does anyone know of any methods that encourage the cell to loose their DNA at various stages of the DNA cycle. Thanks. rifat@icr.ac.uk From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Rifat Hamoudi Newsgroups: bionet.genome.chromosomes Subject: Fluorescent DNA fingerprinting Date: 25 Jul 1996 17:43:00 +0100 Lines: 10 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4t886k$qr3@mserv1.dl.ac.uk> MIME-Version: 1.0 X-Authentication: IMSP Original-To: biochrom@dl.ac.uk Hi, Has anyone tried to carry out flourescent fingerprinting. If so I would appreciate any feedback on the success or otherwise of the experiments. Also whether fingerprinting was done on PACs or YACs? Thanks. Rifat From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!daresbury!is.bbsrc.ac.uk!news.ox.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gwilliam From: gwilliam@hgmp.mrc.ac.uk (Gary Williams) Newsgroups: bionet.genome.chromosomes Subject: Re: genome projects worldwide Date: 25 Jul 1996 08:26:50 GMT Organization: UK HGMP Resource Centre Lines: 11 Message-ID: <4t7b4a$2ts@mercury.hgmp.mrc.ac.uk> References: <4t5jgp$2eh@info.abdn.ac.uk> NNTP-Posting-Host: gallium.hgmp.mrc.ac.uk In article <4t5jgp$2eh@info.abdn.ac.uk>, David D Morgan wrote: >Does anyone have any info about the whereabouts of information regarding the >status of the various genome projects that are going on ? Try http://weber.u.washington.edu/~roach/human_genome_progress2.html for a report on the human genome program, or try looking at the various projects described under http://www.hgmp.mrc.ac.uk/Public/genome-db.html From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!AGR.EHIME-U.AC.JP!Jose From: Jose@AGR.EHIME-U.AC.JP Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome Group for Open Peer Review Date: 24 Jul 1996 19:39:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <9607250247.AA01222@nonami.agr.ehime-u.ac.jp> References: NNTP-Posting-Host: net.bio.net provider@netaxs.com (David W. Bradley, Ph.D.) $B$5$s$O=q$-$^$7$?!'(J > >http://www.journalclub.com/artfr From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!agate!sunsite.doc.ic.ac.uk!sunews!suma3!abrdlher From: Chromosome Terror Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Thu, 25 Jul 1996 01:07:35 +0100 Organization: University of Reading, U.K. Lines: 14 Message-ID: References: <4su6qs$a09@service3.uky.edu> <4t40p4$2ll8@news.doit.wisc.edu> <4t494d$576@mark.ucdavis.edu> NNTP-Posting-Host: suma3-e2.reading.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <4t494d$576@mark.ucdavis.edu> Xref: biosci bionet.molbio.methds-reagnts:47280 bionet.genome.chromosomes:1284 On 24 Jul 1996, Russell Wrobel wrote: > Did anyone ever throw these mysterious super "organism" under the > microscope to actually see if they were cellular? I to once was > filamentous stuff in my azide stock but after a brief look in the > microscope I found that it was definately not cellular but was most > likely some kind of precipitate. > > Russell The one in colchicine forms a nice mycelium, with big vacuoles and no septae. Nach. From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!CRIC.COM!Moir From: Moir@CRIC.COM (Donald Moir) Newsgroups: bionet.genome.chromosomes Subject: Preparation of lambda clone DNA for gridding Date: 25 Jul 1996 09:07:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I'm looking for information on the preparation of lambda clone DNA for high density gridding by robot onto nylon membrances. Is aeration adequate with growth in 96-well microtiter dishes without agitation to obtain good yields of lambda? Are there publications on this topic; most seem to deal with cosmid clones.> ********************************* Donald Moir, Ph.D. Genome Therapeutics Corp. 100 Beaver St. Waltham, MA 02154 tel: 617-893-5007 ext.114 fax: 617-647-3592 Internet: moir@cric.com ********************************* From owner-chromosomes@net.bio.net Wed Jul 24 23:00:00 1996 Path: biosci!rutgers!uwm.edu!newsfeed.internetmci.com!newsreader.sprintlink.net!news.sprintlink.net!news-fw-22.sprintlink.net!news.dca.net!news From: Rich Kiser Newsgroups: bionet.genome.chromosomes Subject: DNA testing Date: Thu, 25 Jul 1996 14:01:59 -0400 Organization: DCANet - Delaware Common Access Network Lines: 24 Message-ID: <31F7B697.755C@itmc.com> NNTP-Posting-Host: 204.183.89.133 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; U) My name is Rich Kiser and I was asked to find out some information related to DNA testing. I am not a scientist, I am just someone who has used the internet for awhile and was asked to find some information. I am not sure if this is an appropriate place to leave this message. If it is not I apoligize and would appreciate some direction in finding people to talk to. Basically, I need to know if a DNA test to determine if a man is the father of two children can be corrupted if the blood sample used for the test was taken the same day the man received 4 units of blood from a transfusion. Apparently, the man was in the hospital being treated for internal bleeding and had received blood over a 6 day period. The day he died he received 4 units and blood samples were taken after the transfusions. These samples were then used to do DNA testing to determine if the man was the true father of the children since he was not married to the mother. The first DNA test said that he was not the father, but everyone knows he was. As you might guess there is litigation pending against the mother from the fathers side of his family. Any help in this matter would be most appreciated and any direction to other resources would be a great help. Rich Kiser From owner-chromosomes@net.bio.net Thu Jul 25 23:00:00 1996 Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Path: biosci!agate!newsgate.duke.edu!news-feed-1.peachnet.edu!usenet.eel.ufl.edu!spool.mu.edu!uwm.edu!news-res.gsl.net!news.gsl.net!hunter.premier.net!newsfeed.internetmci.com!act.news.telstra.net!newshost.telstra.net!news.ci.com.au!metro!metro!news.nsw.CSIRO.AU!news From: Alan Brownlee Subject: Re: Something living on colchicine! X-Nntp-Posting-Host: fungus.prospect.anprod.csiro.au Message-ID: Sender: news@news.nsw.CSIRO.AU Organization: CSIRO Australia References: <31EE7E3C.1148@micro.biol.ethz.ch> Date: Fri, 26 Jul 1996 04:34:11 GMT Lines: 8 Xref: biosci bionet.molbio.methds-reagnts:47330 bionet.genome.chromosomes:1291 quite simple!, colchicine is only nasty to organisms that contain tubulin (microtubule subunit) with the ability to bind bind and be disrupted. Not all eukaryotes, and certainly no bacteria, carry tubulin with the correct sequence. hence colchicine is selective. From owner-chromosomes@net.bio.net Thu Jul 25 23:00:00 1996 Path: biosci!rutgers!uwm.edu!news-res.gsl.net!news.gsl.net!hunter.premier.net!netnews.worldnet.att.net!news.u.washington.edu!root From: dadler@u.washington.edu (David A. Adler) Newsgroups: bionet.genome.chromosomes Subject: Cytogenetics Gallery Update - feedback request Date: 26 Jul 1996 19:07:49 GMT Organization: University of Washington Lines: 25 Message-ID: <4tb525$fb9@nntp3.u.washington.edu> NNTP-Posting-Host: larry.pathology.washington.edu X-Newsreader: NewsFlash [$Revision: 2.230 $] NF-00106-05 Hello all, A visiting summer student from Chalmers University of Technology (Gothenburg), Cecilia Wendin, has remodeled and expanded the UW Pathology's Cytogenetics Gallery. We would now like to get feedback/"beta-testing" and appreciate any comments about content, design, functionality. Especially important is to know if something doesn't work, i.e. bad links and errors in content. You can access the gallery at: http://www.pathology.washington.edu/Cytogallery thank you for your help in testing the new gallery - send email comments to: dadler@u.washington.edu David -- David A. Adler Pathology, Box 357470 University of Washington Seattle, WA 98195 (206) 543-0716 (phone) (206) 543-3644 (fax) http://www.pathology.washington.edu "Science is nothing but trained and organized common sense" T.H.Huxley From owner-chromosomes@net.bio.net Fri Jul 26 23:00:00 1996 Path: biosci!agate!blanket.mitre.org!news.tufts.edu!nntp.uac.net!news.mcb.net!dcs.ed.ac.uk!newsfeed.ed.ac.uk!cardhu!chrisb From: chrisb@hgu.mrc.ac.uk (Chris Boyd) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Followup-To: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Date: 25 Jul 1996 08:53:23 GMT Organization: MRC Human Genetics Unit Lines: 24 Message-ID: <4t7cm3$oml@scotsman.ed.ac.uk> References: NNTP-Posting-Host: cardhu.hgu.mrc.ac.uk X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.molbio.methds-reagnts:47356 bionet.genome.chromosomes:1293 David Grant (dgrant@iastate.edu) wrote: : I once found something (probably bacteria) growing in my 20% SDS in ddH2O E. coli grows quite well in L broth + 20% SDS. In fact, you can use this to cure it of (some) plasmids -- a sort of E. colonic irrigation: author = "M. Tomoeda and M. Inuzuka and N. Kubo and S. Nakamura", title = "{Effective elimination of drug resistance and sex factors in {\it Escherichia coli} by sodium dodecyl sulphate}", journal = "J. Bacteriol.", volume = "95", pages = "1078--1089", year = "1968", I tried this once, but it wasn't as good as acridine orange curing. Best wishes, -- Chris Boyd | from, | MRC Human Genetics Unit chrisb@hgu.mrc.ac.uk | not | Western General Hospital http://www.hgu.mrc.ac.uk/~chrisb | for | Edinburgh EH4 2XU, SCOTLAND From owner-chromosomes@net.bio.net Fri Jul 26 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 27 Jul 1996 02:00:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199607270900.CAA04481@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-chromosomes@net.bio.net Sun Jul 28 23:00:00 1996 Path: biosci!agate!ihnp4.ucsd.edu!munnari.OZ.AU!news.unimelb.EDU.AU!wehi.edu.au!mac174.wehi.edu.au!user From: morahan@wehi.edu.au (Grant Morahan) Newsgroups: bionet.molbio.methds-reagnts,bionet.genome.chromosomes Subject: Re: Something living on colchicine! Date: Mon, 29 Jul 1996 14:04:58 +1000 Organization: WEHI Lines: 11 Message-ID: References: <4su6qs$a09@service3.uky.edu> <4t40p4$2ll8@news.doit.wisc.edu> <4t494d$576@mark.ucdavis.edu> NNTP-Posting-Host: mac174.wehi.edu.au X-Newsreader: Yet Another NewsWatcher 2.0b28 Xref: biosci bionet.molbio.methds-reagnts:47383 bionet.genome.chromosomes:1295 In article , Chromosome Terror wrote: > The one in colchicine forms a nice mycelium, with big vacuoles and no septae. Colchicine is an alkaloid produced by certain plant species. Therefore, it is not at all surprising that some fungi are resistant to it. -- Grant Morahan, Ph.D. The Walter and Eliza Hall Institute of Medical Research From owner-chromosomes@net.bio.net Mon Jul 29 23:00:00 1996 Path: biosci!biosci!not-for-mail From: James Freeman Newsgroups: bionet.general,bionet.genome.chromosomes,bionet.molbio.yeast,bionet.molbio.proteins Subject: Yeast Genome Analysis from the BMERC Date: 30 Jul 1996 13:48:55 -0700 Organization: BioMolecular Resource Center Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <31FE28BA.6F5C@darwin.bu.edu> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.general:22877 bionet.genome.chromosomes:1296 bionet.molbio.yeast:5590 bionet.molbio.proteins:8412 To whom it may concern: The completion of the sequencing of the entire DNA of the S. cerevisae genome, is a major event in the history of biology. All those involved are to be congratulated as we now have the first full genetic blueprint of a "free living" eukaryotic organism. The analysis of these gene products will provide us with a powerful tool for reading the genomes of other eukaryotes, particularly those of higher eukaryotes, which represent the majority of the data currently in the genetic databases. The analysis of the yeast genome is provided a useful framework for the annotation of many of the complete genome projects currently nearing completion, as well as the upcoming human genome. The yeast sequence information used to create this yeast webpage was provided by the GeneQuiz Consortium and the Mips Genome Commission . We have made an initial attempt to integrate these two data structures as well as supplement their annotation with that obtained >From a set of functionally diagnostic patterns (Adams, R. M., et al. Protein Science 5, 1240-49, 1996). In addition we have constructed a data structure of yeast sequence probable homolog clusters or cliques. To aid those desiring to use this new resource we constructed these search tools: User sequence as query (via blast). http://bmerc-www.bu.edu/protein-seq/wwwblast.html User keyword as query. http://bmerc-www.bu.edu/protein-seq/yeast-keyword-search.html Unix egrep regular expression as a sequence query. http://bmerc-www.bu.edu/protein-seq/yeast-egrep-search.html These tools are freely usable to non-commercial individuals and institutions. Commercial users may sample the service twice. Commercial entities wishing to use this service can contact jfreeman@darwin.bu.edu for more information. -- Jim Freeman P: mammon@tiac.net W: jfreeman@darwin.bu.edu Programmer/Analyst at Bio-Molecular Engineering Center at BU Etiam unum capillum umbram suam habet. http://www.tiac.net/users/mammon/index.html From owner-chromosomes@net.bio.net Tue Jul 30 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!online.no!Norway.EU.net!EU.net!howland.reston.ans.net!Germany.EU.net!main.Germany.EU.net!fu-berlin.de!news.th-darmstadt.de!uni-erlangen.de!uni-regensburg.de!news.uni-stuttgart.de!news.urz.uni-heidelberg.de!usenet From: saffrich@embl-heidelberg.de (Rainer Saffrich) Newsgroups: bionet.genome.chromosomes Subject: ANNOUNCE: EU Advanced Workshop in Biotechnology - DNA Sequencing: Advanced Approaches, Automated Methods and Analysis Date: Wed, 31 Jul 1996 17:50:00 GMT Organization: EMBL Lines: 74 Message-ID: <4to682$2qn@sun0.urz.uni-heidelberg.de> NNTP-Posting-Host: pc-ansorge40.embl-heidelberg.de X-Newsreader: Forte Free Agent 1.0.82 EU Advanced Workshop in Biotechnology DNA Sequencing: Advanced Approaches, Automated Methods and Analysis European Molecular Biology Laboratory (EMBL) Heidelberg, Germany November 13 - 23, 1996 Organizers: W. Ansorge, H. Voss, J. Zimmermann, C. Schwager Speakers and Instructors: W. Ansorge (EMBL), V. Benes (EMBL), M. Berks (Cambridge), C. Cantor (Boston), B. Canard (Nice), G. Casari (EMBL), R. Eritja (EMBL), J. Gebert (Heidelberg), J. Hoheisel (Heidelberg), M. Jones (Cambridge), T. Kristensen (Oslo), U. Landegren (Uppsala), S. Oliver (Manchester), S. Pääbo (München), H.K. Schackert (Dresden), M. Uhlen (Stockholm), C. Schwager (EMBL), H. Voss (EMBL), S. Wiemann (Heidelberg), J. Zimmermann (EMBL). The objective of the course is to teach main aspects of DNA sequencing and to show the participants how to carry out DNA sequencing in the framework of their own research projects. The latest methods, strategies and applications will be discussed and extensively practised or demonstrated in the laboratory. Lectures and seminars will complete the program of the course. The course is intended mainly for Ph.D. students and postdoctoral scientists. The selection of participants will be on the basis of their scientific background and the importance of the course techniques for their own research. Practical laboratory course program includes: Random and directed cloning strategies Preparation of template DNA Labelling techniques Cycle sequencing Comparison of sequencing enzymes Automation and robotics Computer assembly and sequence analysis There will be lectures on special topics from invited speakers, blackboard sessions for the practicals and demonstrations and presentations of the participants about their own field of interest and research. The course consists of about 75% practical work and 25% lectures and seminars. For application to the course no forms are necessary, to apply please send only a formal letter including a short c.v., a statement about your current research and a list of recent publications to: Dr. W. Ansorge EMBL Meyerhofstrasse 1 D-69117 Heidelberg GERMANY Telephone: +49-6221-387355 or +49-6221-387574 Telefax: +49-6221-387306 e-mail: ansorge@embl-heidelberg.de or voss@embl-heidelberg.de Deadline for applications is September 15th, 1996. Applications are invited from EU countries. A limited number of grants for travel and accommodation is available for participants. The number of participants is limited to 25. For further information and questions about the course please contact us again.