From owner-chromosomes@net.bio.net Sun Sep 01 23:00:00 1996 Path: biosci!unsw.EDU.AU!R.Passey From: R.Passey@unsw.EDU.AU (Robert Passey) Newsgroups: bionet.genome.chromosomes Subject: Make Your Own Vacuum Pump for Gel Blotting-WEB PAGE Date: 1 Sep 1996 19:35:56 -0700 Organization: UNSW Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <322A47F5.77E8@pop3.unsw.edu.au> Reply-To: r.passey@unsw.EDU.AU NNTP-Posting-Host: net.bio.net A couple of weeks ago I posted an article in this newsgroup relating to a home made vacuum pump suitable for vacuum blotting gels and slot/dot blots. It is very easy to make and costs less than $70.00 Australian. The design for this pump is now availabe on a web page at ftp://sirronald.wustl.edu/pub/mbmiller/vacuum.htm care of Mike Miller at the Washington University School of Medicine, thanks Mike. Comments, suggestions etc can be sent to R.Passey@UNSW.edu.au. Cheers, Robert Passey. From owner-chromosomes@net.bio.net Mon Sep 02 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!newsfeed.internetmci.com!csn!nntp-xfer-1.csn.net!magnus.acs.ohio-state.edu!infoserver.bgsu.edu!bgnet.bgsu.edu!dweaver From: dweaver@bgnet.bgsu.edu (david weaver) Newsgroups: bionet.genome.chromosomes Subject: Human chromosome 14/pulse field electrophoresis Date: 3 Sep 1996 18:17:59 GMT Organization: Bowling Green State University, Bowling Green, OH (USA) Lines: 6 Message-ID: <50hson$b1s@infoserver.bgsu.edu> Reply-To: dweaver@bgnet.bgsu.edu (david weaver) or dweaver@opus.mco.edu NNTP-Posting-Host: falcon.bgsu.edu I presently am looking into the use of pulse field gel electrophoresis methodology to assess breaks points in our generated human tumor cell lines and need help in locating databases for restriction enzyme maps (NotI, etc.) of human chromosome 14. Any and all suggestions would be appreciated. From owner-chromosomes@net.bio.net Tue Sep 03 23:00:00 1996 Path: biosci!POSTBOX.ACS.OHIO-STATE.EDU!yonli001 From: yonli001@POSTBOX.ACS.OHIO-STATE.EDU (Yongzhong Li) Newsgroups: bionet.genome.chromosomes Subject: LOOKING FOR PH D ASSISTANTSHIP IN PLANT GENETICS AND MOLECULAR BIOLOGY Date: 4 Sep 1996 10:36:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 207 Sender: daemon@net.bio.net Distribution: world Message-ID: <199609041728.NAA05628@postbox.acs.ohio-state.edu> NNTP-Posting-Host: net.bio.net Dear Sir: I am very interested in plant genetics and molecular biology and want to look for assistantship to pursuit my Ph D degree. I am specially interested in plant transformation, tissure culture, gene cloning and gene molecular marker, but not limit of those, everything related to plant genetics and molecular biolargy is OK for me, in anywhere and any country. The following is my CV and academic transcripts Name: Yongzhong Li Company: Maize Research Institute, Jilin Academy of agricultural Sciences Address: No. 6, Xixinghua Street, Gongzhuling, Jilin Province, 136100 P. R. China Phone: (434)621-1123 Fax: (434)621-4884 Present Position: Visiting Scholar Company: Department of Horticulture and Crop Science, The Ohio State University Address: 202 Kottman Hall, 2021 Coffey Road, Columbus, OH 43210, USA E-mail: yonli001@postbox.acs.ohio-state.edu Phone: (614)292-9172 Fax: (614)292-7162 EDUCATION: 1982 -- 1985. MS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China. Majored in crop breeding and genetics. Thesis title: Cluster Analysis on Some Of Maine Soybean Parents Sowing In Northeast Of China 1978 -- 1982 BS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China PROFESSIONAL EXPERIECE: 1995.4 -- present, Visiting scholar, Department Of Horticulture And Crop Science, The Ohio State University I have worked on soybean quantitative genetics. I used JMML program to analyze american soybean genetic improvement in the past thirty years among the data of Uniform Test. I am currently working a). In Situ PCR, To determine the chromosome locations of corn 17S rRNA gene and Pox gene as well as some of SSR genes; b). Root morphological differences between the soybean varieties with good flooding resistance and those with bad flooding resistance after different flooding treats 1994.6 -- 1995.4, Visiting scholar, Department Of Agronomy, Kansas State University Studied tissure culture and plant transformation and worked on sorhgum cytogentics for chromosome aberration after different treatments of different herbcides. 1992 -- 1994.6, Associate professor, Maize Research Institute, Jilin Academy Of Agricultural Sciences (JAAS). Jilin province, China. 1987 -- 1991, Assistant professor, Maize Research Institute, JAAS. Jilin province, China. 1985 -- 1986, Research assistant, Soybean Research Institute, JAAS, Jilin province, China. a). 1990 -- 1994, Charged of " Development Of The New Corn Hybrids With High Yield, Resistance To High Population Density And Diseases Sowing In Jilin Province". b), 1994, Charged Of " Development Of New Corn Hybrids Yielding Over 750 kg/mu" . c), 1990 -- 1994, Took part in " Development Of New Corn Hybrids With High Yield, Resistance To Diseases And Good Quality ". ---- National Project. d), 1987 -- 1990, Successfully completed " Development Of Silage Maize Hybrids ". " Jiqing 7 " and " Jiqing 6 " had been released by the National Silage Variety Release Committee and Are being planted by farmers. e). 1985 -- 1987, Took part in " Research On Soybean Male Sterile With Nuclear-cytoplasmic Reaction ". The first invention award was got from the result in Jilin province. The research result have reached advanced level in the world at present. f). 1991, Studied on corn breeding and production in Germany for three months ( from July to October ). PROFESSIONAL SOCIETY MEMBERSHIP China Societyu of Crop Science China Seed Association TECHNICAL SKILLS In Situ PCR, PCR, RAPD, experiences on plant cytogenetics and some on gene cloning and tissure culture , good experiences in plant breeding and genetics, familar with some of other methods on melecular biology as well as different data and word processing software. PUBLICATIONS: 1. Yongzhong Li, 1986, A theoretical research on cluster analysis and its application in plant breeding. Jilin Agricultural Science, No. 2 . 2. Yongzhong Li, 1987, Correlation and path-coefficient analysis of oil and its components in soybean. Soybean Science, No. 3 . 3. Yongzhong Li, 1987, A cluster analysis on some of main soybean parents sowing in northeast of China. Hereditas, No. 3 . 4. Yongzhong Li, 1988, A correlation, stepwise regression and path-coefficient analysis on some of main soybean varieties sowing in northeast of China. Soybean Science, No. 1 . 5. Yongzhong Li, Huazhang Zhang, et. al. , 1990, An analysis of combining ability and genetics potentiality on fine maize inbred lines in northeast of China. Jilin Agricultural Sciences, No. 1 . 6. Yongzhong Li, Weidong Li, et. al. , 1990, A summary of researches on maize root and stalk. Agronomy Abroad, No. 1 . 7. Xinger Liu, Yongzhong Li, et. al. , 1990, An analysis of combining ability on main quantitative characteristics of ten corn inbred lines. Jilin Agricultural Sciences, No. 2 . 8. Yongzhong Li, 1991, A correlation, stepwise regression and path-coefficient analysis on grain yield and its components in maize hybrids. Jilin Agricultural Sciences, No. 1 . TRANSCRIPTS: 1). BS degree courses ( ( course ( exam grade ) ): Advanced Mathematics ( 5 ) Botany ( 5 ) Philosophy ( 5 ) English ( 5) Physics ( 5 ) Mineral Chemistry and Analytical Chemistry ( 5 ) Organic Chemistry ( 5 ) Agro-meteorology ( 5 ) Microbiology ( 5 ) Economics ( 5 ) Biochemistry and Physiology ( 5 ) Soil Science ( 5 ) Agro-entimology ( 4 ) CPC History ( 5 ) Agro-pathology ( 5 ) Genetics ( 4 ) Agricultural Machinary ( 5 ) Biostatistics and Field Experiment ( 4 ) Farming Science ( 5 ) Fertilizer Science ( 5 ) Crop Breeding Science ( 5 ) Crop Cultivation Science ( 5 ) Agro-economics ( 5 ) Graduation Thesis ( 5 ) Graduation Practice ( 5 ) Quantitative Genetics ( 5 ) Seed Science ( 5 ) Application of Modern Physics in Agriculture ( 5 ) Additional Genetics ( 5 ) Additional Farming Science ( 5 ) ** 5 -- excellant; 4 -- good; 3 -- fair; 2 -- fail 2). MS courses ( ( course ( exam grade ) ) Plant Biochemistry ( 92 ) Molecular Genetics ( 95 ) Cytogenetics ( 91 ) Biological Microtechnique ( 98 ) Advanced Breeding Science ( 90 ) Special Subject on Crop Genetic Breeding ( 80 ) Quantitative Genetics ( 99 ) English ( 92 ) Probability and Mathematic Statistics ( 98 ) Linear Algebra ( 95 ) Instrument Analysis ( 92 ) Natural Dialectics ( 85 ) Graduate Seminar ( 90 ) ** Highest mark is 100. The mark less than 60 means failing Thank you very much for your attention, Yongzhong Li From owner-chromosomes@net.bio.net Tue Sep 03 23:00:00 1996 Path: biosci!POSTBOX.ACS.OHIO-STATE.EDU!yonli001 From: yonli001@POSTBOX.ACS.OHIO-STATE.EDU (Yongzhong Li) Newsgroups: bionet.genome.chromosomes Subject: LOOKING FOR PH D ASSISTANTSHIP IN PLANT GENETICS AND MOLECULAR BIOLOGY Date: 4 Sep 1996 10:36:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 207 Sender: daemon@net.bio.net Distribution: world Message-ID: <199609041728.NAA05727@postbox.acs.ohio-state.edu> NNTP-Posting-Host: net.bio.net Dear Sir: I am very interested in plant genetics and molecular biology and want to look for assistantship to pursuit my Ph D degree. I am specially interested in plant transformation, tissure culture, gene cloning and gene molecular marker, but not limit of those, everything related to plant genetics and molecular biolargy is OK for me, in anywhere and any country. The following is my CV and academic transcripts Name: Yongzhong Li Company: Maize Research Institute, Jilin Academy of agricultural Sciences Address: No. 6, Xixinghua Street, Gongzhuling, Jilin Province, 136100 P. R. China Phone: (434)621-1123 Fax: (434)621-4884 Present Position: Visiting Scholar Company: Department of Horticulture and Crop Science, The Ohio State University Address: 202 Kottman Hall, 2021 Coffey Road, Columbus, OH 43210, USA E-mail: yonli001@postbox.acs.ohio-state.edu Phone: (614)292-9172 Fax: (614)292-7162 EDUCATION: 1982 -- 1985. MS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China. Majored in crop breeding and genetics. Thesis title: Cluster Analysis on Some Of Maine Soybean Parents Sowing In Northeast Of China 1978 -- 1982 BS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China PROFESSIONAL EXPERIECE: 1995.4 -- present, Visiting scholar, Department Of Horticulture And Crop Science, The Ohio State University I have worked on soybean quantitative genetics. I used JMML program to analyze american soybean genetic improvement in the past thirty years among the data of Uniform Test. I am currently working a). In Situ PCR, To determine the chromosome locations of corn 17S rRNA gene and Pox gene as well as some of SSR genes; b). Root morphological differences between the soybean varieties with good flooding resistance and those with bad flooding resistance after different flooding treats 1994.6 -- 1995.4, Visiting scholar, Department Of Agronomy, Kansas State University Studied tissure culture and plant transformation and worked on sorhgum cytogentics for chromosome aberration after different treatments of different herbcides. 1992 -- 1994.6, Associate professor, Maize Research Institute, Jilin Academy Of Agricultural Sciences (JAAS). Jilin province, China. 1987 -- 1991, Assistant professor, Maize Research Institute, JAAS. Jilin province, China. 1985 -- 1986, Research assistant, Soybean Research Institute, JAAS, Jilin province, China. a). 1990 -- 1994, Charged of " Development Of The New Corn Hybrids With High Yield, Resistance To High Population Density And Diseases Sowing In Jilin Province". b), 1994, Charged Of " Development Of New Corn Hybrids Yielding Over 750 kg/mu" . c), 1990 -- 1994, Took part in " Development Of New Corn Hybrids With High Yield, Resistance To Diseases And Good Quality ". ---- National Project. d), 1987 -- 1990, Successfully completed " Development Of Silage Maize Hybrids ". " Jiqing 7 " and " Jiqing 6 " had been released by the National Silage Variety Release Committee and Are being planted by farmers. e). 1985 -- 1987, Took part in " Research On Soybean Male Sterile With Nuclear-cytoplasmic Reaction ". The first invention award was got from the result in Jilin province. The research result have reached advanced level in the world at present. f). 1991, Studied on corn breeding and production in Germany for three months ( from July to October ). PROFESSIONAL SOCIETY MEMBERSHIP China Societyu of Crop Science China Seed Association TECHNICAL SKILLS In Situ PCR, PCR, RAPD, experiences on plant cytogenetics and some on gene cloning and tissure culture , good experiences in plant breeding and genetics, familar with some of other methods on melecular biology as well as different data and word processing software. PUBLICATIONS: 1. Yongzhong Li, 1986, A theoretical research on cluster analysis and its application in plant breeding. Jilin Agricultural Science, No. 2 . 2. Yongzhong Li, 1987, Correlation and path-coefficient analysis of oil and its components in soybean. Soybean Science, No. 3 . 3. Yongzhong Li, 1987, A cluster analysis on some of main soybean parents sowing in northeast of China. Hereditas, No. 3 . 4. Yongzhong Li, 1988, A correlation, stepwise regression and path-coefficient analysis on some of main soybean varieties sowing in northeast of China. Soybean Science, No. 1 . 5. Yongzhong Li, Huazhang Zhang, et. al. , 1990, An analysis of combining ability and genetics potentiality on fine maize inbred lines in northeast of China. Jilin Agricultural Sciences, No. 1 . 6. Yongzhong Li, Weidong Li, et. al. , 1990, A summary of researches on maize root and stalk. Agronomy Abroad, No. 1 . 7. Xinger Liu, Yongzhong Li, et. al. , 1990, An analysis of combining ability on main quantitative characteristics of ten corn inbred lines. Jilin Agricultural Sciences, No. 2 . 8. Yongzhong Li, 1991, A correlation, stepwise regression and path-coefficient analysis on grain yield and its components in maize hybrids. Jilin Agricultural Sciences, No. 1 . TRANSCRIPTS: 1). BS degree courses ( ( course ( exam grade ) ): Advanced Mathematics ( 5 ) Botany ( 5 ) Philosophy ( 5 ) English ( 5) Physics ( 5 ) Mineral Chemistry and Analytical Chemistry ( 5 ) Organic Chemistry ( 5 ) Agro-meteorology ( 5 ) Microbiology ( 5 ) Economics ( 5 ) Biochemistry and Physiology ( 5 ) Soil Science ( 5 ) Agro-entimology ( 4 ) CPC History ( 5 ) Agro-pathology ( 5 ) Genetics ( 4 ) Agricultural Machinary ( 5 ) Biostatistics and Field Experiment ( 4 ) Farming Science ( 5 ) Fertilizer Science ( 5 ) Crop Breeding Science ( 5 ) Crop Cultivation Science ( 5 ) Agro-economics ( 5 ) Graduation Thesis ( 5 ) Graduation Practice ( 5 ) Quantitative Genetics ( 5 ) Seed Science ( 5 ) Application of Modern Physics in Agriculture ( 5 ) Additional Genetics ( 5 ) Additional Farming Science ( 5 ) ** 5 -- excellant; 4 -- good; 3 -- fair; 2 -- fail 2). MS courses ( ( course ( exam grade ) ) Plant Biochemistry ( 92 ) Molecular Genetics ( 95 ) Cytogenetics ( 91 ) Biological Microtechnique ( 98 ) Advanced Breeding Science ( 90 ) Special Subject on Crop Genetic Breeding ( 80 ) Quantitative Genetics ( 99 ) English ( 92 ) Probability and Mathematic Statistics ( 98 ) Linear Algebra ( 95 ) Instrument Analysis ( 92 ) Natural Dialectics ( 85 ) Graduate Seminar ( 90 ) ** Highest mark is 100. The mark less than 60 means failing Thank you very much for your attention, Yongzhong Li From owner-chromosomes@net.bio.net Wed Sep 04 23:00:00 1996 Path: biosci!CCR.DSI.UANL.MX!pearl From: pearl@CCR.DSI.UANL.MX ("Dr. Paul R.Earl") Newsgroups: bionet.genome.chromosomes Subject: Biomx, electronic journal-----Call for papers Date: 5 Sep 1996 14:26:39 -0700 Organization: UANL Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <322F628A.16E7@ccr.dsi.uanl.mx> NNTP-Posting-Host: net.bio.net Please see http://www.uanl.mx/biomx or enter Biomx in Yahoo search to get the HomePage. Thank you for your kind attention. Best Wishes, Dr Paul R Earl From owner-chromosomes@net.bio.net Thu Sep 05 23:00:00 1996 Path: biosci!sfu.ca!jwei From: jwei@sfu.ca (Jun-Zhi Wei) Newsgroups: bionet.genome.chromosomes Subject: Chromosome sizes? Date: 5 Sep 1996 18:03:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199609060103.SAA10490@fraser> NNTP-Posting-Host: net.bio.net Could anyone tell me the actual sizes (how many microns) of double minute chromosomes, the largest and the smallest chromosomes in metaphase human and mouse cells? Thank you. J.-Z. Wei From owner-chromosomes@net.bio.net Sat Sep 07 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!www.nntp.primenet.com!nntp.primenet.com!uunet!in2.uu.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: Eukaryotic mRNA Intron/Exon Boundary Consensus Sequences? Date: 8 Sep 1996 04:38:59 GMT Organization: University of Washington, Seattle Lines: 25 Message-ID: <50til3$6v8@nntp3.u.washington.edu> NNTP-Posting-Host: saul3.u.washington.edu NNTP-Posting-User: roach Can anyone point me to a good reference? All I know is that functional introns usually begin with GT and end with AG. Are there known exceptions? How rare are they? In short, given a random intron, what is the probability distribution for the composition of its first and last two base pairs? Thanks, Jared EXTRA BONUS QUESTION: Does this probability distribution depend on the species? ------------------------------------------------------------------ Jared C. Roach Department of Molecular Biotechnology Health Sciences Building, Room K354 University of Washington Box 357730 Seattle, WA 98195 phone (206) 616-4536 FAX (206) 685-7301 roach@u.washington.edu http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Sun Sep 08 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!bunyip.cc.uq.oz.au!newshost.gu.edu.au!s843382 From: s843382@its.gu.edu.au ( DANIEL J. VALE) Newsgroups: bionet.genome.chromosomes Subject: Re: Eukaryotic mRNA Intron/Exon Boundary Consensus Sequences? Date: 9 Sep 1996 01:06:57 GMT Organization: Griffith University, Brisbane, Australia Lines: 36 Message-ID: <50vqjh$nfr@griffin.itc.gu.edu.au> References: <50til3$6v8@nntp3.u.washington.edu> NNTP-Posting-Host: frodo.student.gu.edu.au roach@u.washington.edu (Jared Roach) writes: These marking sequences are called consesus sequences. They are actually quite a bit longer than two base pairs and the degree of similarity to the consensus controlls how efficently the gene is expressed. The actually sequences you could look up in a good 2nd yr genetics text like 'Genetics' by Weaver and Hendrick? (I think thats right but I don't have it with me at the moment) Various organisms differ in which bases they use in preference when either would do so I imagine that it would vary among organisms. > Can anyone point me to a good reference? >All I know is that functional introns usually begin with GT >and end with AG. Are there known exceptions? How rare are >they? In short, given a random intron, what is the probability >distribution for the composition of its first and last two >base pairs? >Thanks, Jared >EXTRA BONUS QUESTION: Does this probability distribution >depend on the species? >------------------------------------------------------------------ >Jared C. Roach >Department of Molecular Biotechnology >Health Sciences Building, Room K354 >University of Washington >Box 357730 >Seattle, WA 98195 >phone (206) 616-4536 >FAX (206) 685-7301 >roach@u.washington.edu >http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Sun Sep 08 23:00:00 1996 Path: biosci!daresbury!is.bbsrc.ac.uk!Andy.Law From: Andy.Law@bbsrc.ac.uk (Andy Law) Newsgroups: bionet.genome.chromosomes Subject: Re: Eukaryotic mRNA Intron/Exon Boundary Consensus Sequences? Date: Mon, 09 Sep 1996 09:42:55 +0100 Organization: Roslin Institute Lines: 47 Message-ID: References: <50til3$6v8@nntp3.u.washington.edu> NNTP-Posting-Host: pc0734.ri.bbsrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.2.0b13 Jared, In article <50til3$6v8@nntp3.u.washington.edu>, roach@u.washington.edu (Jared Roach) wrote: > Can anyone point me to a good reference? > > All I know is that functional introns usually begin with GT > and end with AG. Are there known exceptions? How rare are > they? In short, given a random intron, what is the probability > distribution for the composition of its first and last two > base pairs? Try the following as a starting point. Use ENTREZ to look them up and find related papers. There are probably more up-to-date references available. Jackson, I.J. (1991) A reappraisal of non-consensus mRNA splice sites. Nucleic Acids Research 19, 3795-3798. Mount, S.M. (1982) A catalogue of splice junction sequences. Nucleic Acids Research 10, 459-472. Ohshima, Y. & Gotoh, Y. (1987) Signals for the selection of a splice site in pre-mRNA: Computer anaysis of splice junction sequences and like sequences. Journal of Molecular Biology 195, 247-259. Shapiro, M.B. & Senapathy, P. (1987) RNA splice junctions of different classes of eukaryotes: Sequence statistics and functional implications in gene expression. Nucleic Acids Research 15, 7155-7174. > > Thanks, Jared > > EXTRA BONUS QUESTION: Does this probability distribution > depend on the species? > > Yes. Andy Law ------------------ ( Andy.Law@bbsrc.ac.uk ) ( Big Nose in Edinburgh ) From owner-chromosomes@net.bio.net Wed Sep 11 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!vixen.cso.uiuc.edu!news.indiana.edu!news From: gilbertd@bio.indiana.edu (Don Gilbert) Newsgroups: bionet.molbio.genbank,bionet.genome.chromosomes Subject: looking for peptidyl transferase, eiF2a, eiF3a, Age-1 sequences? Date: 12 Sep 1996 20:46:52 GMT Organization: Biology, Indiana University - Bloomington Lines: 17 Message-ID: <519srs$gej@dismay.ucs.indiana.edu> NNTP-Posting-Host: chipmunk.bio.indiana.edu Xref: biosci bionet.molbio.genbank:2351 bionet.genome.chromosomes:1331 Does anyone know if the following sequences are available to the public? peptidyl transferase eiF2A eiF3A Age-1 (can't remember chromosome point) in mouse Not in bacteria, but in human if possible for the first 3. I'm asking this for a biologist here at IU. Thanks for any pointers, Don -- -- d.gilbert--biocomputing--indiana u--bloomington--gilbertd@bio.indiana.edu From owner-chromosomes@net.bio.net Thu Sep 12 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!swrinde!nntp.primenet.com!news.sprintlink.net!news-peer.sprintlink.net!newsfeed.internetmci.com!news.stealth.net!newsfeed.uk.ibm.net!02-newsfeed.univie.ac.at!paladin.american.edu!news.jhu.edu!welchlink.welch.jhu.edu!ahawkins From: "ANITA L. HAWKINS" Newsgroups: bionet.genome.chromosomes Subject: Re: Chromosome sizes? Date: Wed, 11 Sep 1996 14:03:48 -0400 Organization: HCF - Johns Hopkins University, Baltimore, Maryland, USA Lines: 29 Message-ID: References: <199609060103.SAA10490@fraser> NNTP-Posting-Host: 128.220.59.78 Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <199609060103.SAA10490@fraser> On 5 Sep 1996, Jun-Zhi Wei wrote: > Could anyone tell me the actual sizes (how many microns) of double minute > chromosomes, the largest and the smallest chromosomes in metaphase human > and mouse cells? Thank you. > J.-Z. Wei Hi, This is not really an easy question... length (in microns) depends on the stage of contraction of the chromosomes. For instance, there is roughly a 20:1 reduction in apparent length between prophase chromosomes and the same chromosomes after an overnight colchicine incubation (they'll look like little cubes instead of rods). This is not a change in the length of the DNA per se, just a change in packing. Overall, mouse chromosomes are "shorter" than human, I'd guess (from memory here) the longest mouse chromosome to be about 1/3 the length of the longest human chromosome, at roughly the same level of condensation. But again, there is no "standard" to measure them against. DMs (double minutes) can also vary quite a bit in size, from dust-like to chunky little mini-chromosomes. Even within a single cell line/tumor. Hope this is some help... Anita Hawkins Oncology Cytogenetics The Johns Hopkins Hospital Baltimore, Maryland, USA From owner-chromosomes@net.bio.net Mon Sep 16 23:00:00 1996 From: 1956mishmash@msn.com (Michele Michaelson) Subject: Chromonsones 22 and 11 Date: 17 Sep 96 12:40:21 -0700 Message-ID: <0000336b+0000019e@msn.com> Path: biosci!agate!spool.mu.edu!newspump.sol.net!www.nntp.primenet.com!nntp.primenet.com!newsxfer2.itd.umich.edu!news.sprintlink.net!news-chi-8.sprintlink.net!news.msn.com!msn.com Newsgroups: bionet.genome.chromosomes Organization: The Microsoft Network (msn.com) Lines: 7 i am looking for information about the above. My daughter is mentally handicapped and she has abnormality in these chromosones. she looks normal and is diagnosed autistic. has anyone heard of similar cases or does anyone know of where would be a good place to look. Doctor's advice has been vague and inconclusive and i would like to try to find out for myself if there is a connection. please e mail to 1956mishmash.msn.com. thanks From owner-chromosomes@net.bio.net Tue Sep 17 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!bullwinkle.ucdavis.edu!not-for-mail From: szmillon@bullwinkle.ucdavis.edu (Leon Millon) Newsgroups: bionet.genome.chromosomes Subject: test Date: 17 Sep 1996 20:53:29 GMT Organization: University of California, Davis Lines: 4 Message-ID: <51n349$60c@mark.ucdavis.edu> NNTP-Posting-Host: bullwinkle.ucdavis.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Sorry, just testing.... . From owner-chromosomes@net.bio.net Wed Sep 18 23:00:00 1996 Path: biosci!POSTBOX.ACS.OHIO-STATE.EDU!yonli001 From: yonli001@POSTBOX.ACS.OHIO-STATE.EDU (yongzhong li) Newsgroups: bionet.genome.chromosomes Subject: LOOKING FOR ASSISTANTSHIP FOR GRADUATE STUDY IN PLANT GENETICS AND MELECULAR BIOLOGY Date: 19 Sep 1996 14:23:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 205 Sender: daemon@net.bio.net Distribution: world Message-ID: <199609192117.RAA07342@postbox.acs.ohio-state.edu> NNTP-Posting-Host: net.bio.net Dear Sir/Madam: I, as a visiting scholar from China, have been working or studying in USA for over two years. Now I am posting this message for application of graduate assistantship in plant genetics and molecular biology. I am perticularly interested in the tissue culture, gene cloning, DNA marker and plant transformation, but not limit of these, every research field related to plant genetics or molecular biology is OK for me. any country is OK for me also. Please look at my CV and academic transcripts in the following. Name: Yongzhong Li ( Male ) Date of birth: March 16, 1959 Company: Maize Research Institute, Jilin Academy of agricultural Sciences Address: No. 6, Xixinghua Street, Gongzhuling, Jilin Province, 136100 P. R. China Phone: (434)621-1123 Fax: (434)621-4884 Present Position: Visiting Scholar Company: Department of Horticulture and Crop Science, The Ohio State University Address: 202 Kottman Hall, 2021 Coffey Road, Columbus, OH 43210, USA E-mail: yonli001@postbox.acs.ohio-state.edu Phone: (614)292-9172 Fax: (614)292-7162 EDUCATION: 1982 -- 1985. MS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China. Majored in crop breeding and genetics. Thesis title: Cluster Analysis on Some Of Maine Soybean Parents Sowing In Northeast Of China 1978 -- 1982 BS in Agronomy. Department of Agronomy, Jilin Agricultural University. Changchun, Jilin province, China PROFESSIONAL EXPERIENCES: 1994.6 -- present, Visiting scholar, 1). 1995.4 -- present: Department Of Horticulture And Crop Science, The Ohio State University I have worked on soybean quantitative genetics. I used JMML program to analyze american soybean genetic improvement in the past thirty years among the data of Uniform Test. I am currently working a). In Situ PCR, To determine the chromosome locations of corn 17S rRNA gene and Pox gene as well as some of SSR genes; b). Root morphological differences between the soybean varieties with good flooding resistance and those with bad flooding resistance after different flooding treats 2). 1994.6 -- 1995.4, Department Of Agronomy, Kansas State University Studied tissue culture and plant transformation and worked on sorhgum cytogentics for chromosome aberration after different treatments of different herbicides. 1992 -- 1994.6, Associate professor, Maize Research Institute, Jilin Academy Of Agricultural Sciences (JAAS). Jilin province, China. 1987 -- 1991, Assistant professor, Maize Research Institute, JAAS. Jilin province, China. 1985 -- 1986, Research assistant, Soybean Research Institute, JAAS, Jilin province, China. a). 1990 -- 1994, Charged of " Development Of The New Corn Hybrids With High Yield, Resistance To High Population Density And Diseases Sowing In Jilin Province". b), 1994, Charged Of " Development Of New Corn Hybrids Yielding Over 750 kg/mu" . c), 1990 -- 1994, Took part in " Development Of New Corn Hybrids With High Yield, Resistance To Diseases And Good Quality ". - National Project. d), 1987 -- 1990, Successfully completed " Development Of Silage Maize Hybrids ". " Jiqing 7 " and " Jiqing 6 " had been released by the National Silage Variety Release Committee and Are being planted by farmers. e). 1985 -- 1987, Took part in " Research On Soybean Male Sterile With Nuclear-cytoplasmic Reaction ". The first invention award was got from the result in Jilin province. The research result has reached advanced level in the world at present. f). 1991, Studied on corn breeding and production in Germany for three months ( from July to October ). PROFESSIONAL SOCIETY MEMBERSHIP China Societyu of Crop Science China Seed Association TECHNICAL SKILLS In Situ PCR, PCR, RAPD, experiences on plant cytogenetics and some on gene cloning and tissue culture , very good experience in plant breeding and genetics, familiar with some of other methods on melecular biology as well as different data and word processing software. PUBLICATIONS: 1. Yongzhong Li, 1986, A theoretical research on cluster analysis and its application in plant breeding. Jilin Agricultural Science, No. 2 . 2. Yongzhong Li, 1987, Correlation and path-coefficient analysis of oil and its components in soybean. Soybean Science, No. 3 . 3. Yongzhong Li, 1987, A cluster analysis on some of main soybean parents sowing in northeast of China. Hereditas, No. 3 . 4. Yongzhong Li, 1988, A correlation, stepwise regression and path-coefficient analysis on some of main soybean varieties sowing in northeast of China. Soybean Science, No. 1 . 5. Yongzhong Li, Huazhang Zhang, et. al. , 1990, An analysis of combining ability and genetics potentiality on fine maize inbred lines in northeast of China. Jilin Agricultural Sciences, No. 1 . 6. Yongzhong Li, Weidong Li, et. al. , 1990, A summary of researches on maize root and stalk. Agronomy Abroad, No. 1 . 7. Xinger Liu, Yongzhong Li, et. al. , 1990, An analysis of combining ability on main quantitative characteristics of ten corn inbred lines. Jilin Agricultural Sciences, No. 2 . 8. Yongzhong Li, 1991, A correlation, stepwise regression and path-coefficient analysis on grain yield and its components in maize hybrids. Jilin Agricultural Sciences, No. 1 . TRANSCRIPTS: 1). BS degree courses ( ( course ( exam grade ) ): Advanced Mathematics ( 5 ) Botany ( 5 ) Philosophy ( 5 ) English ( 5) Physics ( 5 ) Inorganic Chemistry and Analytical Chemistry ( 5 ) Organic Chemistry ( 5 ) Agro-meteorology ( 5 ) Microbiology ( 5 ) Economics ( 5 ) Biochemistry and Physiology ( 5 ) Soil Science ( 5 ) Agro-entimology ( 4 ) CPC History ( 5 ) Agro-pathology ( 5 ) Genetics ( 4 ) Agricultural Machinary ( 5 ) Biostatistics and Field Experiment ( 4 ) Farming Science ( 5 ) Fertilizer Science ( 5 ) Crop Breeding Science ( 5 ) Crop Cultivation Science ( 5 ) Agro-economics ( 5 ) Graduation Thesis ( 5 ) Graduation Practice ( 5 ) Quantitative Genetics ( 5 ) Seed Science ( 5 ) Application of Modern Physics in Agriculture ( 5 ) Additional Genetics ( 5 ) Additional Farming Science ( 5 ) ** 5 -- excellant; 4 -- good; 3 -- fair; 2 -- fail 2). MS courses ( ( course ( exam grade ) ) Plant Biochemistry ( 92 ) Molecular Genetics ( 95 ) Cytogenetics ( 91 ) Biological Microtechnique ( 98 ) Advanced Breeding Science ( 90 ) Special Subject on Crop Genetic Breeding ( 80 ) Quantitative Genetics ( 99 ) English ( 92 ) Probability and Mathematic Statistics ( 98 ) Linear Algebra ( 95 ) Instrument Analysis ( 92 ) Natural Dialectics ( 85 ) Graduate Seminar ( 90 ) ** Highest mark is 100. The mark less than 60 means failing Thank you very much for your attention, Yongzhong Li From owner-chromosomes@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!www.nntp.primenet.com!nntp.primenet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!in1.uu.net!newsfeed.pitt.edu!quadra950.pathology.pitt.edu!user From: mcgorry@med.pitt.edu (Michael C. Gorry) Newsgroups: bionet.genome.chromosomes Subject: Chromosome specific libraries Date: 20 Sep 1996 16:59:46 GMT Organization: Univ of Pittsburgh, Center for Genomic Science Lines: 9 Message-ID: NNTP-Posting-Host: quadra950.pathology.pitt.edu I was wondering if anyone was aware of any available human chromosome specific libraries. I am aware of the Hind III and EcoRI libraries at ATCC but wanted to see if there were any more around. I am particularly interested in chromosomes 6,7,8,9,&10. -- Michael Gorry mcgorry@med.pitt.edu From owner-chromosomes@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!uwm.edu!news-peer.gsl.net!news.gsl.net!portc01.blue.aol.com!news-e2a.gnn.com!pop.gnn.com!MPhelps985 From: MPhelps985@gnn.com (Mark Phelps) Newsgroups: bionet.genome.chromosomes Subject: A question from a layman Date: Thu, 19 Sep 1996 20:42:55 Organization: GNN Lines: 23 Message-ID: <51u3hk$6qh@news-e2b.gnn.com> NNTP-Posting-Host: 45-199.client.gnn.com Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-GNN-NewsServer-Posting-Date: 20 Sep 1996 12:43:32 GMT X-Mailer: GNNmessenger 1.3 Scenario: A family on the way to a funeral. The young child in the back seat of the car is able to describe the cemetary; but she's never been there before, never even been past there before. Question: Is it possible for parental genes to contain not only the seed for the child's memory system, but actual memories themselves? Dilemma: I have no way of testing this theory. I have no science background. (It does seem that this theory is far more provable than simply calling the above scenario "paranormal" or "psychic ability". Even if it is disproven, at least it CAN BE disproven, unlike "psychic" claims.) Would someone please take a few minutes and offer some suggestions? As you do, remember my lack of scientific experience (in ANY field). Thanks Mark Phelps From owner-chromosomes@net.bio.net Fri Sep 20 23:00:00 1996 Path: biosci!agate!news.ucdavis.edu!info.ucla.edu!csulb.edu!news.sgi.com!news1.best.com!news.thenet.net!uunet!in2.uu.net!news00.sunet.se!sunic!mn6.swip.net!mn5.swip.net!news From: "Jan Andersson" Newsgroups: bionet.genome.chromosomes Subject: The 16:th chromosome Date: 21 Sep 1996 20:47:56 GMT Organization: - Lines: 8 Message-ID: <01bba7fe$f1e6ff00$2b69f482@win95.swipnet.se> NNTP-Posting-Host: dialup105-3-3.swipnet.se NNTP-Posting-User: s-58370 X-Newsreader: Microsoft Internet News 4.70.1155 Hello! This i a new subject to me so I´m sorry if this is a "stupid" question. My wife´s sister just became a mother and there seems to be a problem with the 16:th cromosome. The doktors are very secretive so that´s why I´m trying to get some info this way. Does anyone know what problems the 16:th chromosome could cause? Jan Andersson From owner-chromosomes@net.bio.net Fri Sep 20 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!www.nntp.primenet.com!nntp.primenet.com!cs.utexas.edu!newshost.convex.com!newsgate.duke.edu!news.duq.edu!newsfeed.pitt.edu!newsflash.concordia.ca!feed.umontreal.ca!jason!not-for-mail From: meilleus@MAGELLAN.UMontreal.CA (Meilleur Sebastien) Newsgroups: bionet.genome.chromosomes Subject: Re: A question from a layman Date: 21 Sep 1996 05:39:13 GMT Organization: Universite de Montreal Lines: 35 Distribution: world Message-ID: <51vv21$lv3@epervier.CC.UMontreal.CA> References: <51u3hk$6qh@news-e2b.gnn.com> NNTP-Posting-Host: magellan.umontreal.ca X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Mark Phelps (MPhelps985@gnn.com) wrote: : Scenario: A family on the way to a funeral. The young child in : the back seat of the car is able to describe the cemetary; but : she's never been there before, never even been past there before. : : Question: Is it possible for parental genes to contain not only : the seed for the child's memory system, but actual memories : themselves? : : Dilemma: I have no way of testing this theory. I have no science : background. (It does seem that this theory is far more provable : than simply calling the above scenario "paranormal" or "psychic : ability". Even if it is disproven, at least it CAN BE disproven, : unlike "psychic" claims.) : : Would someone please take a few minutes and offer some suggestions? : As you do, remember my lack of scientific experience (in ANY : field). : : Thanks : : Mark Phelps : Here's my idea about it. I'd say it's not possible. Genes are modified by evolution, not by memories. I think memories do not get "crystallized" in genes. At least I never saw anything anywhere about that. It is a possibility but nothing scientific ever got close to it as far as I know. I am no doctor in biology but I wanted to state my opinion on the subject. I hope some other people let us know of their opinions. It is an interesting question to think about. Sebastien Meilleur, Biochemistry student, Quebec, canada From owner-chromosomes@net.bio.net Sat Sep 21 23:00:00 1996 From: 1956mishmash@msn.com (Michele Michaelson) Subject: Chromosomes 11 and 22 Date: 17 Sep 96 19:14:58 -0700 Message-ID: <0000336b+0000019f@msn.com> Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!news1.good.net!news.good.net!service-2.agate.net!news.sprintlink.net!news-dc-10.sprintlink.net!news.msn.com!msn.com Newsgroups: bionet.genome.chromosomes Organization: The Microsoft Network (msn.com) Lines: 11 Dear Dr Emanuel Thank you for responding so quickly to my message. My little girl is eight now and has severe learning difficulties as well as behaviour problems. I would be very interested to find out if she does indeed have one of the disorders you are studying. I am going to look at the links you have given me and see where to go from there. I found out about this newsgroup accidentally and perhaps it was beshert. Thanks again. Michele Michaelson [London] From owner-chromosomes@net.bio.net Sun Sep 22 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in3.uu.net!news.con2.com!usenet From: jarrettm@con2.com Newsgroups: bionet.genome.chromosomes Subject: cDNA Expression Library Date: 23 Sep 1996 22:18:33 GMT Organization: Connect2 InterNet News Site Lines: 7 Message-ID: <5272bp$8v1@news.con2.com> NNTP-Posting-Host: port41.con2.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) I am student at Stuyvesant High school and involved in research at Memorial Sloan-Kettering Cancer Center in New York City. I need to do some research on cDNA Expression Libraries. Can somebody please give me a starting point. Thanks. Please e-mail me back at n-jarrett@juno.com instead of jarrettm@con2.com. From owner-chromosomes@net.bio.net Mon Sep 23 23:00:00 1996 Path: biosci!agate!howland.erols.net!EU.net!Norway.EU.net!online.no!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!daresbury!is.bbsrc.ac.uk!news From: castells@yacorba (castells) Newsgroups: bionet.genome.chromosomes Subject: Re: The 16:th chromosome Date: 24 Sep 1996 11:40:04 GMT Organization: IACR, Rothamsted Lines: 25 Message-ID: <528hak$ee8@is.bbsrc.ac.uk> References: <01bba7fe$f1e6ff00$2b69f482@win95.swipnet.se> NNTP-Posting-Host: yacorba.res.bbsrc.ac.uk "Jan Andersson" wrote in article <01bba7fe$f1e6ff00$2b69f482@win95.swipnet.se> : >I >IHello! >IThis i a new subject to me so I´m sorry if this is a "stupid" question. My >Iwife´s sister just became a mother and there seems to be a problem with the >I16:th cromosome. The doktors are very secretive so that´s why I´m trying to >Iget some info this way. Does anyone know what problems the 16:th chromosome >Icould cause? >I >I Jan Andersson >I Hi There is a web site which allow to search for anomalies on chromosomes. it is on URL: http://www.hgmp.mrc.ac.uk/DHMHD/hum_chrome1.html Unfortunatly, it is a bit of a medical site: Lot of specialist's vocabulary. Worth a try anyway. Nath. From owner-chromosomes@net.bio.net Mon Sep 23 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!comp.vuw.ac.nz!ak.netlink.co.nz!auckland.ac.nz!waikato!wave.co.nz!wh13-13.wave.co.nz!user From: macnetcw@wave.co.nz Newsgroups: bionet.genome.chromosomes Subject: genomic bac libraries Date: Tue, 24 Sep 1996 21:59:45 +1200 Organization: Wave Internet Services Lines: 12 Message-ID: NNTP-Posting-Host: wh13-13.wave.co.nz Genomic libraries / Help request We are constructing a genomic library from radiata pine in bac's. No idea as yet of how to best store the library long term. Can anybody help, please? C Walter NZ Forest Research Institute Rotorua New Zealand E mail: Walterc@FRI.CRI.NZ From owner-chromosomes@net.bio.net Tue Sep 24 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!swrinde!news.uh.edu!rpenny From: rpenny@Bayou.UH.EDU (Riggs Penny) Newsgroups: bionet.genome.chromosomes Subject: Textbook recommendation wanted Date: 25 Sep 1996 17:14:53 GMT Organization: University of Houston Lines: 6 Message-ID: <52bpad$pq2@Masala.CC.UH.EDU> NNTP-Posting-Host: bayou.uh.edu X-Newsreader: TIN [version 1.2 PL2] I'm looking for a good textbook for an undergrad upper-level elective cytogenetics/medical genetics class. Any recommendations? Thanks, Penny From owner-chromosomes@net.bio.net Tue Sep 24 23:00:00 1996 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!csn!nntp-xfer-1.csn.net!magnus.acs.ohio-state.edu!ts26-2.homenet.ohio-state.edu!user From: snyder.9@osu.edu (Pam Snyder) Newsgroups: bionet.genome.chromosomes Subject: Re: A question from a layman Date: Tue, 24 Sep 1996 20:45:05 -0500 Organization: Just me Lines: 31 Message-ID: References: <51u3hk$6qh@news-e2b.gnn.com> NNTP-Posting-Host: ts26-2.homenet.ohio-state.edu In article <51u3hk$6qh@news-e2b.gnn.com>, MPhelps985@gnn.com (Mark Phelps) wrote: > Scenario: A family on the way to a funeral. The young child in > the back seat of the car is able to describe the cemetary; but > she's never been there before, never even been past there before. > > Question: Is it possible for parental genes to contain not only > the seed for the child's memory system, but actual memories > themselves? > > Dilemma: I have no way of testing this theory. I have no science > background. (It does seem that this theory is far more provable > than simply calling the above scenario "paranormal" or "psychic > ability". Even if it is disproven, at least it CAN BE disproven, > unlike "psychic" claims.) > > Would someone please take a few minutes and offer some suggestions? > As you do, remember my lack of scientific experience (in ANY > field). > > Thanks > > Mark Phelps The worst thing about the possibility of parents passing memories to children are that memories are not reality but _perceived_ reality, talk about screwing up a child, if memories could be transmitted from parent to offspring prenatally they would have knowledge of situations and circumstances (or the parents perception of them) that parents would never dream of vocalizing!! From owner-chromosomes@net.bio.net Thu Sep 26 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Sep 1996 02:00:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199609270900.CAA01710@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Fri Sep 27 23:00:00 1996 Path: biosci!faseb.org!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!news-peer.gsl.net!news.gsl.net!portc01.blue.aol.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: norsie@aol.com (NORSIE) Newsgroups: bionet.genome.chromosomes Subject: Who does chromosome mapping? Date: 27 Sep 1996 22:16:50 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 17 Sender: root@newsbf02.news.aol.com Message-ID: <52i1qi$pfk@newsbf02.news.aol.com> Reply-To: norsie@aol.com (NORSIE) NNTP-Posting-Host: newsbf02.mail.aol.com I was hoping someone would be able to tell me how to go through chromosome mapping. I really don't know where to begin. Any information would be helpful. I have a very limited science/biology background. (Unfortunately they didn't require science courses in my MBA program) My husband was told by a doctor in the air force that he should go through chromosome mapping before having children because he has some unusual characteristics like drooping eyes and a broad chest. He is also a fraternal twin and both children had serious health problems when they were born. The doctor said my husband's twin may have an extra chromosome, leaving my husband with one less. Does this sound reasonable? Where can I find more information on this subject? What kind of a doctor would do chromosome mapping? I don't imagine there are too many people in my situation so I would appreciate any direction on this issue. Thank you in advance. From owner-chromosomes@net.bio.net Mon Sep 30 23:00:00 1996 Path: biosci!faseb.org!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!news.sgi.com!rutgers!mcrcr6!deustp01 From: deustp01@mcrcr6.med.nyu.edu (Peter D'Eustachio) Newsgroups: bionet.genome.chromosomes Subject: Re: Electrophoresis Message-ID: <1996Oct1.084934.8535@mcrcr6> Date: 1 Oct 96 08:49:34 EDT References: <52pqa1$98p@sleepy.inch.com> Organization: NYU Medical Center, New York, NY 10016, USA Lines: 28 In article <52pqa1$98p@sleepy.inch.com>, bme@metric.inch.com (Gary Silberman) writes: > Are you interested in any of the following products: .... > Bio Medical Equipment (http://www.inch.com/~bme) would like > to help build the right product for you. > > We have been specializing in custom made products for over > fifteen years. > ... > Sincerely, > Gary D. Silberman > President of Bio Medical Equipment > > "Smart, State of the Art" - http://www.inch.com/~bme > bme@inch.com > As long as Mr. Silberman is using our non-commercial space to advertise his commercial products, let me use it to note that I tried to do business with him about ten years ago. I found his products to be of poor quality and his billing practices were, in my opinion, fraudulent. Peter D'Eustachio NYU Medical Center - Biochemistry From owner-chromosomes@net.bio.net Mon Sep 30 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!newsfeed.internetmci.com!in3.uu.net!sleepy.inch.com!metric!bme From: bme@metric.inch.com (Gary Silberman) Newsgroups: bionet.genome.chromosomes Subject: Electrophoresis Date: 1 Oct 1996 00:57:37 GMT Organization: The Internet Channel Lines: 22 Message-ID: <52pqa1$98p@sleepy.inch.com> NNTP-Posting-Host: inch.com X-Newsreader: TIN [version 1.2 PL2] Are you interested in any of the following products: Custom designed electrophoresis apparati, gel combs, and radiation shielding? Bio Medical Equipment (http://www.inch.com/~bme) would like to help build the right product for you. We have been specializing in custom made products for over fifteen years. Check out our web page or e-mail bme@inch.com to request more information. We make the promise that you will not be dissapointed. Sincerely, Gary D. Silberman President of Bio Medical Equipment "Smart, State of the Art" - http://www.inch.com/~bme bme@inch.com