From owner-chromosomes@net.bio.net Wed Oct 02 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.erols.net!EU.net!news.eunet.fi!newsmaster From: mtg Newsgroups: bionet.genome.chromosomes Subject: Q: Choromosome 9 length ? (more info) Date: Thu, 03 Oct 1996 15:13:27 +0300 Lines: 21 Message-ID: <3253ADE7.7C5F@neste.com> NNTP-Posting-Host: manager.kni.neste.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (X11; I; SunOS 5.4 sun4m) I wrote earlier: >Case: After amniocentesis (during the 16th week of pregnancy) >and chromosome mapping one "branch" (don't know which) of >chromosome number 9 was found to be longer than the other one >next to it. Could someone please tell me in layman's terms is >there usually an excess of genes or are genes somehow "streched" >in order the branch to be longer ? >How common is this ? > >If one of the parents has the similar deflection and if he/she >is diagnosed not to have any abnormalities what is the overall >probability that the deflection causes abnormalities to the fetus ? >Is there an estimate whether this deflection will affect the >fetus/child if none of the parents has a longer branch in >chromosome 9 ? Should this longer branch be DNA-mapped(?) as well ? I got some more info: The "longer" branch is on the p-side, where there seems to be a tiny extension (g-stripe(?)) near 9p13 (or 9p1.3 , can't understand the terminology). Thanks again From owner-chromosomes@net.bio.net Wed Oct 02 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.erols.net!EU.net!news.eunet.fi!newsmaster From: mtg Newsgroups: bionet.genome.chromosomes Subject: Q: Choromosome 9 length ? Date: Thu, 03 Oct 1996 12:19:44 +0300 Lines: 16 Message-ID: <32538530.4444@neste.com> NNTP-Posting-Host: manager.kni.neste.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (X11; I; SunOS 5.4 sun4m) Case: After amniocentesis (during the 16th week of pregnancy) and chromosome mapping one "branch" (don't know which) of chromosome number 9 was found to be longer than the other one next to it. Could someone please tell me in layman's terms is there usually an excess of genes or are genes somehow "streched" in order the branch to be longer ? How common is this ? If one of the parents has the similar deflection and if he/she is diagnosed not to have any abnormalities what is the overall probability that the deflection causes abnormalities to the fetus ? Is there an estimate whether this deflection will affect the fetus/child if none of the parents has a longer branch in chromosome 9 ? Should this longer branch be DNA-mapped(?) as well ? Thank You From owner-chromosomes@net.bio.net Wed Oct 02 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!sn.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!flamenco!slonim From: slonim@flamenco.wi.mit.edu (Donna Slonim) Newsgroups: bionet.genome.chromosomes Subject: WICGR Mouse Genomic Mapping Project, Data Release 12 Date: 3 Oct 1996 19:13:57 GMT Organization: Whitehead Institute for Biological Research Lines: 181 Sender: slonim@flamenco (Donna Slonim) Message-ID: <53139l$369@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: flamenco.wi.mit.edu ---------------------------------------------------------------------------- WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH MOUSE GENOMIC MAPPING PROJECT Data Release October 1996 ---------------------------------------------------------------------------- GENETIC MAP The July 1996 Data Release was the final release of the Mouse Genetic Map at the Whitehead Institute/MIT Center for Genome Research. It reflects the data represented in the 14 March, 1996 issue of Nature. Please see this paper, and also Genetics 131:423-447 (1992) for descriptions of the materials and methods used to construct the maps. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. ---------------------------------------------------------------------------- MOUSE PHYSICAL MAPPING PROJECT Since completing the genetic map, our goal is to construct a physical map of the mouse, consisting of 10,000 markers screened against a mouse YAC library. Many of the markers will be the SSLPs that are also on the genetic map, but we will also be adding random STSs to the physical map to reach a total of 10,000 markers. In this release, we include data for 4,208 markers successfully screened against the YAC library. 4,006 of these markers have been placed into physcial map contigs. The table below shows the number of physically and genetically-mapped markers on each chromosome. The right-most columns count the number of markers shared by the two maps ("BOTH"), and the total number of distinct markers mapped in any way ("TOTAL MARKERS"). Breakdown of Mapped Markers by Chromosome CHROMOSOME PHYSICAL GENETIC BOTH TOTAL MARKERS chr1 371 511 323 559 chr2 344 513 314 543 chr3 188 342 166 364 chr4 195 348 188 355 chr5 237 401 226 412 chr6 209 367 200 376 chr7 209 356 207 358 chr8 233 350 227 356 chr9 205 336 193 348 chr10 185 293 179 299 chr11 253 350 248 355 chr12 203 278 200 281 chr13 248 302 241 309 chr14 175 259 170 264 chr15 202 264 195 271 chr16 150 214 140 224 chr17 75 254 74 255 chr18 123 231 122 232 chr19 83 134 83 134 chrX 118 228 109 237 TOTAL 4006 6331 3805 6532 YAC Library The YAC library being used is described in an upcoming issue of Mammalian Genome (Haldi, et al.). It consists of 40,000 YACs with an average insert size of 820 kb. The entire library, or individual YAC clones, is available through Research Genetics, Huntsville, Alabama (800-533-4363 phone, 800-336-9014 FAX) and Genome Systems, Inc. of St. Louis, Missouri, (phone 800-430-0030), and also through the Mouse Genome Centre, Oxfordshire, England. We are currently using a subset of 24,000 clones in building the physical map. These 24,000 clones are arrayed here at the Center in a 5-dimensional pooling scheme. The STSs are screened on these 5D pools, with 3 hits describing a single YAC address, and up to two extra hits confirming that address. STSs screened Please note that this release of the physical map represents only a first pass through the genome. It includes only those SSLPs that worked well on the first try, and did not require any special attention. In later releases we plan to include as many of the SSLPs as can be successfully screened, as well as ESTs, random STSs and other markers. The genetically-mapped SSLPs are screened on the 5-dimensional pools, using the same pair of primers as were used when we placed them on the genetic map. If the marker fails on the first try, it is run through the screening process a second time. If it fails twice, we attempt to convert the SSLP into an STS by picking two new primers and an internal oligo from one side or the other side of the CA-repeat. Markers that fail this final step will probably not be placed on the physical map. If you are working with an SSLP that you want to know the precise placement of in relation to other SSLPs nearby, you may wish to consult the European Collaborative Interpecific Mouse Backcross. They are mapping our SSLPs in a high-resolution 1000-animal backcross. Chromosomes 2, 11, 15, 16, and 17 are completed, chromosomes 3 and 4 are nearly done, and others are underway. Many of the markers on both the genetic and physical map are available through Research Genetics, at a reduced cost under a community discount arrangement set up by the WICGR. (Note: WI/MIT CGR has placed the markers in the public domain. The center and its personnel receive no financial benefit from the sale of primers.) Citing this data Data releases occur on a quarterly basis or more frequently if the amount of new data warrants it. At the end of each quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within two weeks of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy is among the most rapid, broad and regular of any genome center. Its purpose is to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. References to this data in publications should be cited by listing each of the following three sources: 1. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. 2. Dietrich, W.F. et al. (1994) A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genetics 7:220-245. 3. Copeland, N.G., D.J. Gilbert, N.A. Jenkins, J.H. Nadeau, J.T. Eppig, L.J. Maltais, J.C. Miller, W.F. Dietrich, R.G. Steen, S.E. Lincoln, A. Weaver, D.C. Joyce, M. Merchant, M. Wessel, H. Katz, L.D. Stein, M.P. Reeve, M.J. Daly, R.D. Dredge, A. Marquis, N. Goodman, E.S. Lander (1993) Genome Maps IV. Science 262:67. 4. Supplemented by additional markers in: Whitehead Institute/MIT Center for Genome Research, Genomic Map of the Mouse, Database Release 10, June 1996. Additional publications with related information: Dietrich, W., J. Miller, H. Katz, D. Joyce, R. Steen, S. Lincoln, M. Daly, M.P. Reeve, A. Weaver, P. Anagnostopoulos, N. Goodman, N. Dracopoli, E.S. Lander (1992) Genetic Maps. Stephen J. O'Brien, ed. Cold Spring Harbor Laboratory Press. Assay Conditions for the Genetic Map: Assay conditions and other experimental details are given in: Dietrich, W., et al., 1992. Genetics 131: 423-447. Briefly, the PCR protocol used radiolabeled primers in a 25 cycle PCR (1 '94 degrees, 2' 55 degrees, 3' 72 degrees) on 20 ng of genomic DNA. CONTENTS OF THIS DIRECTORY 10-96.MarkerInfo.txt PCR primer and product size 10-96.GeneticMapInfo.txt Genetic mapping information 10-96.PhysicalMapInfo.txt Physical mapping information 10-96.STS2Contig.txt Contig information 10-96.Contig2STS.txt Contig information - inverted Sequences.txt Raw source sequences for STSs pictures/ Maps in postscript and Macintosh format For further information contact: Donna Slonim, slonim@genome.wi.mit.edu; or Joyce Miller, jmiller@genome.wi.mit.edu Last modified October 2, 1996 From owner-chromosomes@net.bio.net Mon Oct 07 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.erols.net!news.sgi.com!esiee.fr!jussieu.fr!oleane!in2p3.fr!swidir.switch.ch!01-newsfeed.univie.ac.at!news.cesnet.cz!rhino.cis.vutbr.cz!risc.upol.cz!decsys.vsb.cz!newsmast From: Respondby@Mail.com (LSAT Productions) Newsgroups: bionet.genome.chromosomes Subject: !!!!!!!!!!! FREE INTERNET ACCESS !!!!!!!!!!! Date: Fri, 04 Oct 1996 08:22:27 GMT Organization: Home.com Lines: 74 Message-ID: <532h8j$73v@decsys.vsb.cz> Reply-To: Respondby@Mail.com NNTP-Posting-Host: cust104.max22.los-angeles.ca.ms.uu.net X-Newsreader: Forte Agent NS#8 1.0.82 !!!!!!!!!!! FREE INTERNET ACCESS !!!!!!!!!!! NEVER EVER pay for Internet Access AGAIN!!! E-V-E-R! Amazing Course on Audio Tape describes STEP by STEP what your Internet Service Provider doesn't want you to know! * How to get FREE LOCAL DIAL-UP PPP Internet Access! * How to Surf the Web,Newsgroups,and EMAIL Anonymously/Untraceable! * How to Make VOICE Phone Calls ANYWHERE in the World UNTRACEABLE! * Where you can get FREE Email Remailing * Where you can get FREE Email Addresses * Where you can get FREE Access to News Servers * How to get FREE Internet Tools for Email, News, WWW, Etc. * How to get free accounts on BBS's * MUCH MUCH More!!! No matter where you live in the world we guarantee you will get FREE internet access legally and anonymously! TO ORDER: LSAT Productions PO Box 2747-453 DEPT NS-8A Huntington Beach, CA 92648 USA WE ACCEPT Check or Money Order. $24.95 Delivered (WORLDWIDE) (US FUNDS ONLY) Act NOW supplies are in Limited Supply! FAST SERVICE! ORDER FORM - Print out and mail - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Price Each Sub-Total __ Total # of Courses __________ ___________ Shipping (+ 1.00/add. course) ___________ Sales Tax (CA residents 7.75%%) ___________ Order total US $___________ PAYMENT BY: ___ Check ___ Money Order - US FUNDS only! SHIP TO: __________________________ _____________________ Name Phone # ______________________________ Address ______________________________ As it should be written on a mail City,State,Zip piece. ______________________________ Country WE ACCEPT US FUNDS ONLY! Please make checks payable to -> LSAT From owner-chromosomes@net.bio.net Tue Oct 08 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!bunyip.cc.uq.oz.au!news From: Andrea Crampton Newsgroups: bionet.genome.chromosomes Subject: c/s spreads from tick cells Date: Wed, 09 Oct 1996 15:16:44 +0000 Organization: University of Queensland Lines: 9 Message-ID: <325BC1DC.58CB@student.uq.edu.au> Reply-To: s316741@student.uq.edu.au NNTP-Posting-Host: barker.vet.uq.oz.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) hi! is there any one out there doing tick cell culture and preparing chromosome spreads????? if so i would love to get in contact with you to swap ideas.... ia m working on Boophilus microplus.... Hope to hear from you Andrea From owner-chromosomes@net.bio.net Wed Oct 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: dvorah@agri.huji.ac.il (Deborah Weisman) Newsgroups: bionet.genome.chromosomes,bionet.molbio.gene-linkage Subject: INTERNATIONAL COURSE IN QTL DETECTION (corrected) Date: 10 Oct 1996 16:27:46 -0700 Organization: Hebrew University Faculty of Agriculture Lines: 115 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.genome.chromosomes:1360 bionet.molbio.gene-linkage:1188 INTERNATIONAL COURSE IN QTL DETECTION AND MARKER-ASSISTED SELECTION FIRST ANNOUNCEMENT This is the first announcement of an international course in statistical methods of QTL detection and marker-assisted selection. The course will cover all important methods in QTL detection and estimation for plants, animals, and humans. It will also describe methods for marker-assisted selection for both plants and animals. The main emphasis will be on theory, genotyping methods will be considered only briefly. When: August 24 to September 4, 1997 (This is tentative, we might move it up a week by popular demand). Where: Jerusalem, Israel, The Mitzpa Rachel Convention Center, located on the southern outskirts of Jerusalem. The view is spectacular. Instructors: Joel I. Weller, ARO, The Volcani Center Morris Soller, The Hebrew University of Jerusalem Abraham Korol, Haifa University Language of Instruction: English (or a reasonable facsimile) Cost: (If you have to ask, you can't afford it) Price including complete room and board, and a weekend tour of the Galilee will be about $1700 for single occupancy, and $1300 for double occupancy. These prices are NOT final and are subject to change. I hope to be able to offer about ten scholarships for graduate students and nontenured postdocs (If I can get some funding). The scholarships will be for half of the double occupancy costs. Again, more information on this will follow: Acceptance: The course will be limited to 40 participants. It will be assumed that the participants have a good general knowledge of quantitative genetics, basic statistics, matrix algebra, and mixed model methodology. They must also have a "sound background in biology" for example, they must know how many legs a cow has, and what sound a chicken makes. Unless I find that the response in overwhelming, acceptance will be on a first-come, first-serve basis. Syllabus: The tentative course outline is given below. This will definitely change. Correspondence: Please conduct all correspondence directly with me, by either E-mail, FAX, or snail mail, as convenient. My addresses are given below. I should be able to send out more detailed information within the next two months to all interested parties. Meanwhile I would appreciate a response from all individuals seriously considering attending. I hope to set up a homepage for this course soon. -------------------------------------------------------------------------- Joel Ira Weller * /^^\ || /^^\ * Institute of Animal Sciences * /^^^^\||/^^^^\ * A. R. O., The Volcani Center * //^^^/^\/^\^^^\\ * P. O. Box 6 * / | | | | \ * Bet Dagan 50250 * 88 | | 88 * ISRAEL * / | * * / | * E-mail: weller@agri.huji.ac.il * / | * Phone : 972-8-470583/143 * | \ * Fax : 972-8-475075 * | \ * ---------------------------------------------------------------------------- ABBREVIATED COURSE OUTLINE I. Genetic markers and quantitative trait loci, basic concepts A. Historical overview. B. The "usual" assumptions. C. Experimental designs to detect QTL, generation of linkage disequilibrium. II. Statistical methods A. Problems in QTL analysis. B. Analysis objectives. C. Estimation of QTL effects with fixed linear models. D. Maximum likelihood estimation (MLE). E. Other parameter estimation methods. III. Estimation and maximization of statistical power A. Estimation of required experiment size for anova analysis. B. Methods to increase power per individual genotyped. C. Number of markers vs. number of individuals. D. The problem of multiple comparisons. IV. Marker-assisted selection (MAS) A. Advantages and disadvantages of traditional breeding. B. Possible contributions of MAS to within-breed selection. C. Problems with application of MAS. D. Expected gains with application of MAS. -- Deborah Weisman Computer Center Faculty of Agriculture Hebrew University of Jerusalem POB 12 Rehovot 76100 ISRAEL fax:972-8-9466306 ph: 972-8-9481232 dvorah@agri.huji.ac.il From owner-chromosomes@net.bio.net Thu Oct 10 23:00:00 1996 Path: biosci!unifr.ch!Xavier.Gansel From: Xavier.Gansel@unifr.ch (Xavier Gansel) Newsgroups: bionet.genome.chromosomes Subject: Problem with lambda subcloning and sequencing Date: 11 Oct 1996 08:28:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, The aim of this question concern sequencing of lambda DNA. I cloned a genomic fragment in a lambda phage corresponding to the cDNA I am studying. The genomic part corresponding to the ORF have been subcloned and sequenced and I try to go in the promoter walking form the ORF. It turns out that I can't subclone any fragment of this promoter in pUC based plasmid by walking from the 5' end of the ORF. I mean that all the transformant I got are shorter than expected, when I obtain some, and do not hybridize with my probe. I tried to cycle sequence "by hands" from lambda DNA with no results, and I do not have access to an automated sequencer. I want the sequence of this promoter in order to analysis is regulation ... any advices would be greatly appreciated, Thanks in advance, Xavier. Could you please directly reply to my e.mail address, I shall post back a summary of your answers. <><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Xavier Gansel Plant Biology Institute University of Fribourg 3 Rue A. Gockel tel +41 37 29 88 18 / 10 1700 Fribourg fax +41 37 29 97 40 Switzerland e-mail xavier.gansel@unifr.ch <><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> From owner-chromosomes@net.bio.net Sat Oct 12 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.genome.chromosomes Subject: New News Group devoted to Genome Structure/Function Date: 12 Oct 1996 20:54:11 -0700 Organization: McGill Div. of Experimental Medicine Lines: 124 Sender: daemon@net.bio.net Distribution: world Message-ID: <32610FC8.3E44@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Dear Bionet readers, I am posting this note to ask for comments and suggestions for a new news group on genome/chromatin structure and function: tentatively called bionet.genome.structure The importance of chromatin/genomes structure for the processes of replication, transcription and recombination is becoming more and more apparent. Chromatin context can affects the expression and replication timing of a gene domain. Such interelationship between gene function and changes in chromatin structure have been demonstrated through an evolution of techniques from Dnase I sensitivity mapping to fluorescent in situ hybridization (FISH. I believe with such far reaching affects on many areas of molecular biology, it is time we devoted a news group to the study of genome structure and function. The following is a list of "possible" topics. If you feel we should include others or have suggestions as to the format of the news group please reply via e-mail to: dellaire@odyssee.net In addition to myself three tentative discussion leaders have already been contacted and wish to encourage the formation of such a group. They are: Dr. Eric Milot (Erasmus, Neatherlands) Dr. Ronald Hancock (U of Laval, Quebec, Canada) Dr. Peter Cook (Oxford, England) Cheers, Graham Dellaire ++++++++++++++++++ Here is the list of topics so far. 1. Genome/chromatin accessibility and recombination -recombination hotspots (mieotic and mitotic) -fragile sites -imprinting and recombination rates -ectopic gene targeting and chromatin structure 3. Effect of DNA topology/structure(Triple strand, Z-DNA, cruciform, bent etc) on biological processes such as: -replication -transcription -recombination 4. Histones and Nucleosomes and chromatin structure/function -H1 repression of transcription -Post translational modification of histones acetylation (H4, H3), phosphorylation (H1, H3) and ubiquitination (H2A, H2B) -Histone variants (ex. H2A.Z in mammals, H5 of chicken) 5. Models of genome structure (Loop Domain Model, Channel Model, MegaBase Giant Loop Model, etc.) 6. Evolution of the Genome -isochores and base-content (GC vs. AT) -formation of gene clusters and syntenic mapping -repetitive elements (satellites, telomeric and centromeric (alpha) repeats, lines and sines) 7. Biologically important mutants and knockouts that affect genome/chromatin structure -ex. SNF/SWI, TOPO mutants in yeast -RAD 51,52,54 knockout mice -AT, BLM, FA mouse models 8. Techniques for genome/chromatin analysis -Fluorescent Insitu Analysis -psoralen, polyamine crosslinking -In vivo nucleosome foot printing -Dnase I/Micrococcal Nuclease sensitivity -VM26 Topoisomerase II site mapping 9. Chromatin/DNA binding proteins and their effects on chromatin structure and/or gene expression -Polycomb proteins -Rap1 (telomere silencing) -alpha2-MCM1 (repression of MAT locus) -CENP A/B/C (centromere structure/function) -XCAP-C/E, SMC1/2 (chromatin Condensation) -remodeling of chromatin by SWI/SNF proteins 10. Matrix attatchent regions (MAR's), domain boundaries and locus control regions (LCR's) and their relationship to gene structure and function. -definition of transcription/replication domains -model systems ex. betaglobin (LCR) SCS/SCS' of the Drosophila Heat Shock Locus (HS87a7) 11. Phenomenon of Position Effect and Transvection -in drosophila (HP1, polycomb, heterochromatization) -in mammalian systems (silencing or variegated expression of transgenes) 12. Epigenetic effects on gene function -imprinting -methylation -maintenance of early/late replication 13. Dosage compensation mechanisms and X chromosome inactivation -MSL proteins of Drosophila -XIC (Xist RNA) in mammals -CpG methylation 14. Chromatin structure and DNA replication -ORC1 protein of yeast 15. DNA repair and chromatin structure -TFIIH (transcription coupled repair) -p53 -BLM and AT genes -poly-ADP-polymerase (PARP) From owner-chromosomes@net.bio.net Sat Oct 12 23:00:00 1996 Path: biosci!agate!howland.erols.net!news2.digex.net!news6.digex.net!news2.dn.net!ppp14.cpod.fr!user From: demouliere@mail.cpod.fr (demoulière) Newsgroups: bionet.genome.chromosomes Subject: Re: c/s spreads from tick cells Date: 13 Oct 1996 21:25:15 GMT Organization: digitalNation Lines: 13 Message-ID: References: <325BC1DC.58CB@student.uq.edu.au> NNTP-Posting-Host: ppp14.cpod.fr A propos de cellules et de chromosomes In article (Dans l'article) <325BC1DC.58CB@student.uq.edu.au>, s316741@student.uq.edu.au wrote (écrivait) : > hi! > is there any one out there doing tick cell culture and preparing > chromosome spreads????? > > if so i would love to get in contact with you to swap ideas.... ia m > working on Boophilus microplus.... > > Hope to hear from you > Andrea From owner-chromosomes@net.bio.net Sat Oct 12 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!howland.erols.net!newsfeed.internetmci.com!newshub.csu.net!charnel.ecst.csuchico.edu!csusac!csus.edu!news From: jbhoppin@sfsu.edu Newsgroups: bionet.genome.chromosomes Subject: for a story on mapping the genome Date: Fri, 11 Oct 1996 14:14:04 -0800 Organization: California State University Sacramento Lines: 19 Message-ID: <325EC6AC.E29@sfsu.edu> NNTP-Posting-Host: @slip-225.sfsu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Hello all, I'm a reporter at San Frnacisco State working on a multimedia story regarding the science and future implications of mapping the human genome. I was wondering if someone could put me in touch with some sources (books, articles, people) to help me understand the process, including what is being mapped, how it is done, who is doing it, the debate about patenting, the Human Genome Diversity Project, how this might change medicine as we know it, how much money is being spent, etc. Anybody who can put this in layman's terms would be an enormous help. In advance, thank you. -Jason jbhoppin@sfsu.edu From owner-chromosomes@net.bio.net Sat Oct 12 23:00:00 1996 Path: biosci!biosci!not-for-mail From: tara@linkage.rockefeller.edu (Tara Cox Matise) Newsgroups: bionet.general,bionet.molbio.gene-linkage,bionet.genome.chromosomes Subject: Human Chr. 1 WWW Page and Genetic Map Date: 12 Oct 1996 21:49:14 -0700 Organization: Rockefeller University Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.general:23585 bionet.molbio.gene-linkage:1189 bionet.genome.chromosomes:1363 1. The home page for the Human Chromosome 1 WWW page has moved to http://linkage.rockefeller.edu/chr1. 2. A Data Page has been added to the Chr1 WWW pages. This page will serve as a link to data provided by members of the chr1 community - this may be published or unpublished data and provides a forum for us to display and view data prior to publication, data that is too lengthy to be published, or data that would not otherwise be readily available to us. The address of the data page is: http://linkage.rockefeller.edu/chr1/chr1data.html 3. The first set of data has been added to the Chr1 Data Pages. I am working on constructing genetic maps of chromosome 1 that contain over 800 markers - basically all the polymorphic markers I can find. The first of these maps is complete and has been made available on the data pages. I call this map a Comprehensive Positional map since it includes all markers, but simply shows the position of these markers relative to the Genethon chromosome 1 map. I am currently expanding the map to add additional markers to the Genethon map wherever possible and will put this on the data page when complete. You can find a link to the genetic map from the Data Page, and the direct address is: http://linkage.rockefeller.edu/chr1/data/genmap/genmap.shtml Sincerely, Tara Matise Lab. of Statistical Genetics Rockefeller University From owner-chromosomes@net.bio.net Sun Oct 13 23:00:00 1996 Path: biosci!BRIC.POSTECH.AC.KR!mhlee From: mhlee@BRIC.POSTECH.AC.KR (mhlee) Newsgroups: bionet.genome.chromosomes Subject: help; DNA sequence of Cotesia glomerata virus (Polydnavirus: Bracoviridae) Date: 13 Oct 1996 18:03:55 -0700 Organization: bric Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <32619236.A39@bric.postech.ac.kr> Reply-To: mhlee@bric.postech.ac.kr NNTP-Posting-Host: net.bio.net Hello! Is there anybody know the DNA sequence of Cotesia glomerata virus? Thanks in advance. Your Sincerely, Min-Ho Lee. Min-Ho Lee; mhlee@bric.postech.ac.kr Web for the Korean Entomologist(my home page); http://bric.postech.ac.kr/about/mhlee/ From owner-chromosomes@net.bio.net Sun Oct 13 23:00:00 1996 Path: biosci!rutgers!gatech!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!ifenton From: ifenton@hgmp.mrc.ac.uk (I. Fenton) Newsgroups: bionet.molbio.gene-linkage,bionet.genome.chromosomes Subject: Re: Curved thumb Date: 14 Oct 1996 18:17:12 GMT Organization: UK HGMP Resource Centre Lines: 18 Message-ID: <53u038$gn@mercury.hgmp.mrc.ac.uk> References: <32627538.0@news.atlas.com.hk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Xref: biosci bionet.molbio.gene-linkage:1192 bionet.genome.chromosomes:1368 TaroLover wrote: >My student is a twin, he has a right curved thumb and left straight >thumb. But his elder btother has a left curved thumb and right >straight thumb. But their father and mother are both right straight >thumb and left straight thumb. >How to explain the above event? >Would any Biology experts can answer my question, please send me a >E-mail. Thank You! :) well, i'm no biology 'expert', but i've done some analysis. the first question for you is are these twins monozygotic or not ? it makes a big difference to the question/answer... Iain. From owner-chromosomes@net.bio.net Sun Oct 13 23:00:00 1996 From: tarolove@interserve.com.hk (TaroLover) Newsgroups: bionet.molbio.gene-linkage,bionet.genome.chromosomes Subject: Curved thumb Date: Mon, 14 Oct 1996 13:36:13 GMT X-Newsreader: Forte Free Agent 1.0.82 NNTP-Posting-Host: 202.84.234.173 Message-ID: <32627538.0@news.atlas.com.hk> Lines: 10 Path: biosci!agate!spool.mu.edu!newspump.sol.net!howland.erols.net!www.nntp.primenet.com!nntp.primenet.com!nntp.uio.no!news.cais.net!news.hkt.net!gtr.hkt.net!news.atlas.com.hk!202.84.234.173 Xref: biosci bionet.molbio.gene-linkage:1191 bionet.genome.chromosomes:1367 My student is a twin, he has a right curved thumb and left straight thumb. But his elder btother has a left curved thumb and right straight thumb. But their father and mother are both right straight thumb and left straight thumb. How to explain the above event? Would any Biology experts can answer my question, please send me a E-mail. Thank You! :) From owner-chromosomes@net.bio.net Mon Oct 14 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: Eukaryotic gene biology Date: 15 Oct 1996 15:35:48 +0100 Lines: 48 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5407g4$dkt@mserv1.dl.ac.uk> X-Sender: tenovus@POP-SERVER.CENT.GLA.AC.UK Original-To: arab-gen@dl.ac.uk, bionews@dl.ac.uk, bphyssoc@dl.ac.uk, celegans@dl.ac.uk, cellbiol@dl.ac.uk, biochrom@dl.ac.uk, gnome-pr@dl.ac.uk Meeting Announcement: I would be grateful if you could distibute this announcement on your network. The 9th Tenovus-Scotland Symposium on Eukaryotic Gene Biology will be held in the University of Glasgow on 9th - 11th April 1997. Programme: Wednesday 1. Comparative Genomics Chairman: Steve Oliver (Manchester) Jean-Louis Gu=E9net (Paris) Steven Jones (Cambridge) Graham Moore (Norwich) Steve Oliver (Manchester) 2. Repetitive DNA Chairman: Keith Johnson (Glasgow) Howard Cooke (Edinburgh) Jean-Louis Mandel (Strasbour= g) Darren Monckton (Glasgow) Bob Wells (Houston) Thursday 3. DNA Repair and Cancer Chairman: Bob Brown (Glasgow) James German (New York) Jan Hoeijmakers (Rotterdam) Paul Modrich (Durham, NC) Medal Lecture Steve Jackson (Cambridge) 4. Membrane Signalling Chairman: Margaret Frame (Glasgow) Ian Kerr (London) Anne Ridley (London) Mike Schaller (Chapel Hill) Richard Treisman (London) =46riday 5. Mitochondria Chairman: John Walker (Cambridge) Chris Leaver (Oxford) Eric Shoubridge (Montreal) .. Alexander Tzagoloff (New York) Michael Yaffe (La Jolla) 6. Cell Biology of the Nucleus Chairman: Alan Wolffe (Bethesda) Bill Earnshaw (Edinburgh) Frank Grosveld (Rotterdam) .. Angus Lamond (Dundee) Alan Wolffe (Bethesda) Registration Deadline 1st January 1997 Booking forms & further information by replying to this e-mail: Tenovus@udcf.gla.ac.uk From owner-chromosomes@net.bio.net Mon Oct 14 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.erols.net!torn!news.ryerson.ca!hopper.acs.ryerson.ca!h2lau From: h2lau@acs.ryerson.ca ("Benny Lau - Aerospace Engineering!!") Newsgroups: bionet.genome.chromosomes Subject: Question on Genetics Date: 14 Oct 1996 20:08:27 GMT Organization: Ryerson Polytechnic University Lines: 8 Message-ID: <53u6jr$e83@ns2.ryerson.ca> NNTP-Posting-Host: hopper.acs.ryerson.ca X-Newsreader: TIN [version 1.2 PL2] Hello, I just have a question on Artificial insemination. I have read somewhere that a father can r canr inpregnate his daughter through AI to produceI to produce genetically compatible fetal material to be inject to a family member whom might have somekind of genetic disorder. I was wondering when is this genetic material is extracted? How is it extracted? Obviously the fetus is not allow to develop, so when is the fetus terminated? From owner-chromosomes@net.bio.net Tue Oct 15 23:00:00 1996 Path: biosci!rutgers!uwm.edu!spool.mu.edu!newspump.sol.net!howland.erols.net!feed1.news.erols.com!uunet!news-in2.uu.net!nntp.earthlink.net!usenet From: mplewinska@earthlink.net (Magdalena Cano Plewinska) Newsgroups: bionet.genome.chromosomes Subject: Re: Question on Genetics Date: Wed, 16 Oct 1996 02:56:30 GMT Organization: Miami, FL, USA Lines: 22 Message-ID: <3264421e.12735439@news.earthlink.net> References: <53u6jr$e83@ns2.ryerson.ca> NNTP-Posting-Host: cust121.max19.miami.fl.ms.uu.net X-Newsreader: Forte Agent .99e/32.227 h2lau@acs.ryerson.ca ("Benny Lau - Aerospace Engineering!!") wrote: >Hello, I just have a question on Artificial insemination. I have read >somewhere that a father can r canr inpregnate his daughter through >AI to produceI to produce >genetically compatible fetal material to be inject to a family member >whom might have somekind of genetic disorder. I was wondering when is >this genetic material is extracted? How is it extracted? Obviously the >fetus is not allow to develop, so when is the fetus terminated? > I am not aware of anyone doing this (overtly). I think that the issue of conceiving a child for the sole purpose of terminating the pregnancy to harvest the tissue raises all sorts of ethical questions. I, for one, find the idea repugnant and I don't think I would go along with any such scheme. I am not against abortion or the use of fetal tissue per se but I am against the use of pregnancy for "tissue farming". - Magdalena M. Plewinska, MD Genetics University of Miami, Miami, FL, USA From owner-chromosomes@net.bio.net Tue Oct 15 23:00:00 1996 Path: biosci!LEONIS.NUS.SG!imatanhc From: imatanhc@LEONIS.NUS.SG (Ms Tan Hui-Cheng) Newsgroups: bionet.genome.chromosomes Subject: POSTDOCTORAL POSITION AVAILABLE Date: 15 Oct 1996 20:04:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITION AVAILABLE - Gene Analysis in Arabidopsis A Research Fellow position is available at the Institute of Molecular Agrobiology, Singapore to work on Arabidopsis. Gene trap and enhancer trap insertional mutagenesis of Arabidopsis has been performed using transposable elements (Sundaresan et al. 1995, Genes and Development 9:1797-1810; Springer et al. 1995, Science 268:877-880; Sundaresan 1996, Trends in Plant Sciences 1:184-190). The transposon insertion lines are being analyzed for reporter gene expression patterns in embryogenesis, and for developmental mutations. Molecular analysis of selected insertions is performed by isolation and sequencing of the DNA flanking the insertions. The Research Fellow position will be primarily concerned with DNA sequencing and mapping of genes isolated using the insertion lines, and subsequent computer analysis utilizing EST, genome and protein databases. This position will come with an attached technician, and access to automated sequencing equipment, as well as computing facilities. Prior experience with DNA sequencing and bioinformatics will be an asset. Research Fellow appointments are normally for a period of three years, with extensions possible at the end of this period. The salary range starts at S$49,680 per annum (US $1 = approx S$1.40),the actual salary depending upon qualifications and experience. Successful applicants may qualify for benefits such as housing subsidies, travel to meetings, annual bonus of one to three months pay, provident fund/gratuity scheme, etc. Applicants should send their CV including names, designations and e mail addresses of 3 referees to: Dr V. Sundaresan Director, Institute of Molecular Agrobiology National University of Singapore 59A The Fleming, 1 Science Park Drive Singapore 118240 Tel: 65-8723339 Fax: 65-8725349 e mail: imav1@nus.sg From owner-chromosomes@net.bio.net Wed Oct 16 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!howland.erols.net!newsfeed.internetmci.com!monster.cibola.net!www.utep.edu!usenet From: Javier Medina Newsgroups: bionet.genome.chromosomes Subject: I have a question Date: Thu, 17 Oct 1996 14:44:40 +0000 Organization: A poorly-installed InterNetNews site Lines: 5 Message-ID: <32664653.7BED@mail.utep.edu> NNTP-Posting-Host: 129.108.37.17 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) I am wondering if anyone could help me with the following question. Q: Considering Robertsonian translocations are stable, how is it that the 4 kinetochores can exist? I would greatly appreciate it if anyone knows. Thank you. From owner-chromosomes@net.bio.net Wed Oct 16 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!uunet!in1.uu.net!hearst.acc.Virginia.EDU!gems.vcu.edu!jwinters From: jwinters@gems.vcu.edu Newsgroups: bionet.genome.chromosomes Subject: Re: Curved thumb Message-ID: <1996Oct17.150939.2539@gems.vcu.edu> Date: 17 Oct 96 15:09:39 -0400 References: <32627538.0@news.atlas.com.hk> Organization: Medical College of Virginia Lines: 26 In article <32627538.0@news.atlas.com.hk>, tarolove@interserve.com.hk (TaroLover) writes: > My student is a twin, he has a right curved thumb and left straight > thumb. But his elder btother has a left curved thumb and right > straight thumb. But their father and mother are both right straight > thumb and left straight thumb. > > How to explain the above event? > > Would any Biology experts can answer my question, please send me a > E-mail. Thank You! :) First, I wouldn't classify myself as a biology expert. Second, are these twins identical or fraternal? If they're fraternal, it wouldn't be suprising that they have differences such as this one. If they are identical, however, this might fall under the category of mirror twinning. This simply means for whatever trait (curved/straight thumbs in this case), the identical twins are mirror images of each other. Identical twins are never perfectly identical anyway. HTH. Jenny Winters Ph.D. candidate Department of Human Genetics Medical College of Virginia From owner-chromosomes@net.bio.net Wed Oct 16 23:00:00 1996 Path: biosci!agate!howland.erols.net!vixen.cso.uiuc.edu!news.uoregon.edu!newsfeed.orst.edu!news.uidaho.edu!usenet From: Biotechnology Lab Newsgroups: bionet.genome.chromosomes Subject: (ABI sequencer Date: Thu, 17 Oct 1996 16:25:59 -0700 Organization: University of Idaho Lines: 2 Distribution: world Message-ID: <3266C087.66B1@uidaho.edu> NNTP-Posting-Host: 129.101.104.251 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Win95; I; 16bit) Does anyone know what company makes the glass plates for the ABI 377 automated DNA sequencer? From owner-chromosomes@net.bio.net Thu Oct 17 23:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!news.york.ac.uk!figaro.york.ac.uk!pdf104 From: PD Foskett Newsgroups: bionet.genome.chromosomes Subject: Degredation Date: Fri, 18 Oct 1996 22:45:25 +0100 Organization: The University of York, UK Lines: 5 Message-ID: NNTP-Posting-Host: figaro.york.ac.uk Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Has anyone got any ideas on the degredation of dna? I have been trying to find articles but am at a loss with such a defined subject. Any hints and tips? From owner-chromosomes@net.bio.net Fri Oct 18 23:00:00 1996 Path: biosci!agate!spool.mu.edu!newspump.sol.net!howland.erols.net!newsfeed.internetmci.com!in3.uu.net!news.iij.ad.jp!nr1.scn.co.jp!sinfony-news!wnoc-tyo-news!aist-nara!wnoc-kyo-news!kuis-news!wsclark!hns!chiba-ns!villon.nig.ac.jp!usenet From: Satoshi OOta Newsgroups: bionet.genome.chromosomes Subject: The Name of Genome Conference Date: Thu, 17 Oct 1996 03:17:57 +0900 Organization: National Institute of Genetics, Japan. Lines: 18 Message-ID: <326526CA.DE9@lab.nig.ac.jp> NNTP-Posting-Host: joker.lab.nig.ac.jp Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Macintosh; I; PPC) Does anyone know the name of international genome conference that will be held in Toronto next March? This conference may have its own home page, but I cannot find it. Thanks. ------------------------------------------------------------ Satoshi OOta (Neither Ota nor Ohta nor Oota, but OOta!) Department of Genetics, School of Life Science, The Graduate University of Advanced Studies at Laboratory of Evolutionary Genetics, National Institute of Genetics, Mishima, 411 Japan ||| FAX: +81-559-81-6794 (@.@) TEL: +81-559-81-6790 _/|\_ ....... ------------------------------------------------------------ From owner-chromosomes@net.bio.net Sat Oct 19 23:00:00 1996 Path: biosci!agate!howland.erols.net!news3.cac.psu.edu!news.cse.psu.edu!news.cc.swarthmore.edu!news.haverford.edu!cas33.resnet.haverford.edu!user From: cgastald@haverford.edu (Cheryl Gastaldo) Newsgroups: bionet.genome.chromosomes Subject: question Date: Sun, 20 Oct 1996 14:59:22 +0100 Organization: Bryn Mawr and Haverford College NetNews Lines: 5 Message-ID: NNTP-Posting-Host: 165.82.108.33 X-Newsreader: Value-Added NewsWatcher 2.0b22.0+ i don't know if this is the right place to ask this, but i'll try anyway. can anyone tell me the chromosonal location of the defect that causes xeroderma pigmentosum? thanks. cheryl From owner-chromosomes@net.bio.net Sun Oct 20 23:00:00 1996 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!news-in2.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rcjohnsen@aol.com (Rcjohnsen) Newsgroups: bionet.genome.chromosomes Subject: Re: question Date: 20 Oct 1996 21:15:39 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <54eirr$q3r@newsbf02.news.aol.com> References: Reply-To: rcjohnsen@aol.com (Rcjohnsen) NNTP-Posting-Host: newsbf02.mail.aol.com Go to the web site http://www3.ncbi.nlm.nih.gov/omim/ and Omim search database. Enter the keyword XP and you will find that XP is a complex disease with 8 different genes scattered thoughout the genome. Hope you dfind what you're looking for. Roger Rcjohnsen@aol.com From owner-chromosomes@net.bio.net Sun Oct 20 23:00:00 1996 Path: biosci!ALU.WUSTL.EDU!rwilson From: rwilson@ALU.WUSTL.EDU (Rick Wilson) Newsgroups: bionet.genome.chromosomes Subject: RE: The Name of Genome Conference Date: 21 Oct 1996 05:09:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <199610211209.HAA11497@geneman.wustl.edu> NNTP-Posting-Host: net.bio.net Satoshi OOta wrote: >Does anyone know the name of international genome conference that will >be held in Toronto next March? This conference may have its own home >page, but I cannot find it. It's the HUGO meeting - HGM '97. See http://hugo.gdb.org/hgm97.htm. RW From owner-chromosomes@net.bio.net Mon Oct 21 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!metro!metro!wabbit.its.uow.edu.au!news From: Peter Gooding Newsgroups: bionet.molbio.gene-linkage,bionet.genome.chromosomes Subject: Re: Curved thumb Date: 22 Oct 1996 00:26:58 GMT Organization: Uni Of Wollongong Lines: 24 Message-ID: <54h4ci$t7b@wabbit.its.uow.edu.au> References: <32627538.0@news.atlas.com.hk> <53u038$gn@mercury.hgmp.mrc.ac.uk> NNTP-Posting-Host: mac3194.uow.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; 68K) X-URL: news:53u038$gn@mercury.hgmp.mrc.ac.uk Xref: biosci bionet.molbio.gene-linkage:1200 bionet.genome.chromosomes:1381 ifenton@hgmp.mrc.ac.uk (I. Fenton) wrote: >TaroLover wrote: >>My student is a twin, he has a right curved thumb and left straight >>thumb. But his elder btother has a left curved thumb and right >>straight thumb. But their father and mother are both right straight >>thumb and left straight thumb. >>How to explain the above event? >>Would any Biology experts can answer my question, please send me a >>E-mail. Thank You! :) > >well, i'm no biology 'expert', but i've done some analysis. the >first question for you is are these twins monozygotic or >not ? it makes a big difference to the question/answer... > >Iain. > Are identical twins mirror images or double images of each other? (I thought it was the latter. However, at some stage in the process they are joined on opposite sides.). Peter. From owner-chromosomes@net.bio.net Mon Oct 21 23:00:00 1996 Path: biosci!CGL.UCSF.EDU!griffen From: griffen@CGL.UCSF.EDU Newsgroups: bionet.genome.chromosomes Subject: Subcloning lambda DNA Date: 22 Oct 1996 10:01:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <326CFD1A.3E7D@cgl.ucsf.edu> Reply-To: m.wilson@socrates.ucsf.edu NNTP-Posting-Host: net.bio.net I am trying to subclone fragments from a lambda dash clone pulled from a mouse genomic library into bluescript. The fragments are between 7 and 13 kb with sticky ends, and i have been purifiying them by galssmilk based methods, or by electroelution, and ligating using the clonetech high efficiency ligation kit. So far i have had no success, and am very frustrated. Any suggestions welcomed. Maria Wilson From owner-chromosomes@net.bio.net Thu Oct 24 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!news-stkh.gsl.net!news.gsl.net!news-peer.gsl.net!news.gsl.net!howland.erols.net!surfnet.nl!news.wau.nl!ns2!ns2!nntp Newsgroups: bionet.genome.chromosomes Subject: Pelargonium mapping Message-ID: <326F5FF0.4930@ato.agro.nl> From: "a.bergmans" Date: Thu, 24 Oct 1996 05:24:16 -0700 Nntp-Posting-Host: ato071.ato.dlo.nl X-Mailer: Mozilla 2.0 (Win16; I) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 4 Does anyone know a group that is involved in the mapping of the Pelargonium genome? A. Bergmans From owner-chromosomes@net.bio.net Thu Oct 24 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!www.nntp.primenet.com!nntp.primenet.com!arclight.uoregon.edu!dispatch.news.demon.net!demon!pplros.demon.co.uk!tomlinson From: Anthony Tomlinson Newsgroups: bionet.molbio.yeast,bionet.genome.chromosomes Subject: Hexamine Cobalt cytotoxicity? Date: Fri, 25 Oct 1996 13:52:17 GMT Organization: PPL Therapeutics Lines: 11 Distribution: world Message-ID: <846251537.22925.0@pplros.demon.co.uk> NNTP-Posting-Host: pplros.demon.co.uk X-NNTP-Posting-Host: pplros.demon.co.uk X-Newsreader: Nuntius 2.0.1_68K X-XXMessage-ID: X-XXDate: Fri, 25 Oct 1996 14:53:24 GMT Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit Xref: biosci bionet.molbio.yeast:6023 bionet.genome.chromosomes:1385 I have been reading a paper by Kovacic et al (NAR 23 (19), 3999-4000, 1995) where Co(NH3)6Cl3 was used to protect large DNA from shearing. Does anyone know anything about the cytotoxicity of this compound, especially in mammalian cells (I'm thinking about YAC transfection and also microinjection). Thanks, Anthony. From owner-chromosomes@net.bio.net Thu Oct 24 23:00:00 1996 Path: biosci!EXTRA.NCHGR.NIH.GOV!benton From: benton@EXTRA.NCHGR.NIH.GOV (David Benton) Newsgroups: bionet.genome.chromosomes Subject: Science/NCBI Human Transcript Maps Date: 25 Oct 1996 08:49:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <9610251553.AA15192@extra.nchgr.nih.gov> NNTP-Posting-Host: net.bio.net With thanks to Andy Baxevanis of NCHGR who wrote the original version of this posting. Note: neither Andy nor I are authors of the paper described below, so perhaps it is not our place to call it to your attention. But, perhaps the authors are too busy or modest to do so... FYI: An online version of Science's "The Human Transcript Map" wall chart was made available yesterday on NCBI's Web site. The URL for the site is: http://www.ncbi.nlm.nih.gov/SCIENCE96/ In addition to information and graphics from the wall chart, there are details on "featured genes", a glossary of terms, and search engines for browsing OMIM and the definition lines of GenBank records corresponding to genes mapped in this study. Also, maps can be searched to get a list of all cDNAs corresponding to a specific region of the genome. Full text of the accompanying article (October 25 issue; Greg Schuler, Mark Boguski, et al.) is also available; the abstract follows. ABSTRACT: The human genome is thought to harbor 50,000 to 100,000 genes, of which about half have been sampled to date in the form of expressed sequence tags. An international consortium was organized to develop and map gene-based sequence tagged site markers on a set of two radiation hybrid panels and a yeast artificial chromosome library. More than 16,000 human genes have been mapped relative to a framework map that contains about 1000 polymorphic genetic markers. The gene map unifies the existing genetic and physical maps with the nucleotide and protein sequence databases in a fashion that should speed the discovery of genes underlying inherited human disease. The integrated resource is available through a site on the World Wide Web at http://www.ncbi.nlm.nih.gov/SCIENCE96/. [Science, Volume 274, Issue of 25 October 1996, pp. 540-546] -David Benton, NCHGR From owner-chromosomes@net.bio.net Sat Oct 26 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Oct 1996 02:00:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199610271000.CAA03993@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Tue Oct 29 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!solace!news.stealth.net!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!news-e2a.gnn.com!pop.gnn.com!sanchezjos From: sanchezjos@gnn.com (Josh S) Newsgroups: bionet.genome.chromosomes Subject: Chromosome 15 Date: Tue, 29 Oct 1996 23:40:12 Organization: GNN Lines: 8 Message-ID: <556ilp$iuf@news-c1.gnn.com> NNTP-Posting-Host: 24-242.client.gnn.com Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-GNN-NewsServer-Posting-Date: 30 Oct 1996 03:39:37 GMT X-Mailer: GNNmessenger 1.3 I am writing a paper on mapping a genetic disease on chromosome 15. Does anyone have any suggestions about any diseases. I am also looking for Genetics resources on the WWW. Any help will be greatly appreciated. Please email me thank you Casanova From owner-chromosomes@net.bio.net Tue Oct 29 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!uunet!in3.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: ssihouston@aol.com (Ssihouston) Newsgroups: bionet.genome.chromosomes Subject: PH.D. SENIOR SCIENTIST WANTED Date: 30 Oct 1996 15:14:32 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 50 Sender: root@newsbf02.news.aol.com Message-ID: <558cv8$frv@newsbf02.news.aol.com> Reply-To: ssihouston@aol.com (Ssihouston) NNTP-Posting-Host: newsbf02.mail.aol.com Biotechnology company in Houston, Texas has an open position as follows. Job Title: Senior Research Scientist-Biologist Reports to: Research Director (Ph.D.) Summary: Develop and supervise a cell/molecular biology-based research program to further our understanding of the interaction of nucleic acid-derived therapeutics with cells. Develop in vitro and in vivo assay systems and analytical methods to test the activity of oligonucleotides and other therapeutics. Communicate research findings to other scientists and management, and contribute to the strategic research direction of the company. Working within the framework provided by the management, explore the full range of biological research methods to achieve Company goals. Requirements: 1. Must be in the Houston area, no relocation money available (or willing to relocate at your own expense). 2. Ph.D. in cell biology, molecular biology, or biochemistry. 3. 2-4 years of experience postdoctoral. 4. 4-8 years of experience in fundamental research on biochemistry and molecular biology in the fields of immunology or cancer biology. 5. Broad-based training and experience in the development, implementation, and interpretation of in vitro assays. 6. Publications in major, peer reviewed scientific journals. 7. Excellent communication skills. 8. Personal computer skills. 9. Highly motivated TEAM PLAYER. Salary: Commensurate with experience, probable range $45-50K/annum. If you are interested in this position, please submit your resume to the address below. We will furnish a further skill survey for potential candidates to complete to determine qualifications. If you know someone who might be interested in this position, please provide them with a copy of the this message. David W. Walters, Ph.D. Senior Scientific Recruiter ScienStaff, Inc. ssihouston@aol.com http://www.net-solutions.com/ScienStaff 7007 Gulf Freeway, Suite 239 Houston, Texas 77087 Phone: 713-640-1929, FAX: 713-640-1942 From owner-chromosomes@net.bio.net Tue Oct 29 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: ssihouston@aol.com (Ssihouston) Newsgroups: bionet.genome.chromosomes Subject: JOB - MOLECULAR BIOLOGIST WANTED Date: 30 Oct 1996 15:16:23 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 37 Sender: root@newsbf02.news.aol.com Message-ID: <558d2n$fti@newsbf02.news.aol.com> Reply-To: ssihouston@aol.com (Ssihouston) NNTP-Posting-Host: newsbf02.mail.aol.com DOCTORAL-LEVEL MOLECULAR BIOLOGISTS (2) A leading international producer of oligonucleotides, peptides, genes and molecular biology products for the research and industrial communities has the following position open for two Ph.Ds. The successful candidates will be project leaders developing products/kits for our molecular biology product line. RESEARCH SCIENTIST POSITION for our R&D Division. Applicants should have a Ph.D. with 3-5 years experience or equivalent in Molecular Biology. Responsibilities include the development of new product lines and technologies and conducting laboratory benchwork. The position requires innovative thinking and the ability to plan and execute independent research. Practical experience in PCR, DNA sequencing and cloning techniques are required. The successful candidate should be self-motivated with a keen awareness of current literature and technologies, with particular emphasis in the field of "gene regulation . Customer support skills are required. The company is growing into new product areas where the successful candidate will be an integral part of our expansion. An exquisite understanding of molecular biology kit conception, design, manufacture and technical support is a distinct plus (indeed, a near absolute requirement) as is the sincere desire to work at the bench level to produce such kits. Please send curriculum vitae and list of references to: David W. Walters, Ph.D. Senior Scientific Recruiter ScienStaff, Inc. ssihouston@aol.com http://www.net-solutions.com/ScienStaff 7007 Gulf Freeway, Suite 239 Houston, Texas 77087 Phone: 713-640-1929, FAX: 713-640-1942 From owner-chromosomes@net.bio.net Wed Oct 30 22:00:00 1996 Path: biosci!biosci!not-for-mail From: lester@genome.wi.mit.edu (Lester Hui) Newsgroups: bionet.genome.chromosomes Subject: WHITEHEAD/MIT HUMAN PHYSICAL MAP, RELEASE 11 Date: 30 Oct 1996 16:01:57 -0800 Organization: Whitehead Institute for Biological Research Lines: 100 Sender: daemon@net.bio.net Distribution: world Message-ID: <558alg$fct@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: net.bio.net ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH HUMAN GENOMIC MAPPING PROJECT DATA RELEASE 11 (OCTOBER 1996) The eleventh release of data from the Human Physical Mapping Project at the Whitehead Institute/MIT Genome Center, covering data generated through the end of October, 1996, is now available. This data release contains YAC screening data for 22,582 sequence tagged sites (STSs) screened on the CEPH mega-YAC library. For each STS, we report addresses for the YACs found to contain the STS. The data assemble into 292 contigs using single linkage between STSs. In addition, we also report a radiation hybrid map of the genome containing 12508 STS markers mapped on the Genebridge Panel, as well as integrations of the genetic, radiation hybrid and YAC contig maps. The map includes data from over 10000 expressed sequences, part of the transcript map published in Science 25 October 1996. The data is available electronically in two ways. ANONYMOUS FTP: The entire data release is available as a set of Microsoft Excel files and tab-delimited ascii files on our ftp server. Using an ftp client (such as "Fetch" on the Macintosh), connect to ftp-genome.wi.mit.edu Use "anonymous" as your user name, and give your e-mail address as your password. The data files are present in the directory /distribution/human_STS_releases/oct96. The contents are as follows: 10-96.INTRO.txt Introduction to the data release, in straight text format 10-96.INTRO.html The same in HTML (World Wide Web) format 10-96.STS.DATA.txt STS mapping data as tab-delimited text. chromosomes/ The same, split into smaller chromosome-specific files 10-96.YAC2STS.txt Inverse map of YAC to STS screening data 10-96.CONTIG2STS.txt YAC contig lists. 10-96.CONTIG2STS.txt The same, inverted. 10-96.SEQUENCES.txt Full sequences of STSs developed in-house. 10-96.MAPPED.EST.txt Cross reference between ESTs, IMAGE clones and map positions. 10-96.ALIASES.txt Cross reference of STS names. pictures/ Pictures of integrated maps in Macintosh and PS forms. rhmap/ Radiation hybrid maps. genmap/ Genethon genetic linkage maps. The data is also available in compressed form using the gzip program. THE WORLD-WIDE WEB: You will need a World Wide Web client such as Mosaic (Unix, MS-Windows and Macintosh) or MacWeb (Macintosh). Instruct your client to connect to http://www.genome.wi.mit.edu/ >From there, follow the "Human Physical Mapping Project" link. You will be able to browse and download the raw data set, view the individual and integrated maps, and to get information on the radiation hybrid and contig analyses. All mapped STSs are available through the Genome Database (GDB) and through GenBank. Users interested in EST mapping are also referred to the following URL: http://www.ncbi.nlm.nih.gov/Science96/ QUESTIONS AND PROBLEMS. If users have any questions or problems, please contact us at human_STS_help@genome.wi.mit.edu We invite suggestions about how to make these data release most useful. DATA RELEASE POLICY AND CITATION. Data releases are scheduled quarterly. At the end of each quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within a week of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy aims to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. The information for the human genome mapping project should be cited as: Whitehead Institute/MIT Center for Genome Research, Human Physical Mapping Project, Data Release 11 (October 1996). From owner-chromosomes@net.bio.net Wed Oct 30 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in3.uu.net!newsfeed.pitt.edu!scramble.lm.com!news From: "Soon Paik" Newsgroups: bionet.genome.chromosomes Subject: Job-cancer translational research Date: 31 Oct 1996 03:27:13 GMT Organization: Preferred Company Lines: 53 Message-ID: <01bbc6d9$ec162de0$f726abcc@paiks> NNTP-Posting-Host: paiks.slip.lm.com X-Newsreader: Microsoft Internet News 4.70.1155 Research Positions available: The Pathology Section of the National Surgical Adjuvant Breast and Bowel Project (NSABP) located at Allegheny General Hospital in Pittsburgh has several openings as follows to establish a new reseasrch laboratory under the supervision of Dr. Soon Paik. Fundings for all positions are secure for four years. All positios are for American Citizens or Permanent residents only, except for a post-doctoral position, which is open to any qualified investigator. We seek highly motivated young researchers who wishes to be at the forefront of translational research connected to a large multicenter clinical trial group. Especillay of interest are those with experience in positional cloning, CGH, or phage display, or transgenic systems. Available Positions: one senior research assistant (opening now) - molecular genetics two junior research assistants (opening January 1997) - molecular genetics or immunology one post-doctoral fellow (opening now) - molecular genetics one breast pathology fellow (opening January 1997) - board certified or eligible pathologist with firm molecular biology training (preferably finishing the first post-doc fellowship) who wants to develop as an academic breast pathologist. Main Projects: 1. Transcriptional repressor function of estrogen receptor in breast cancer cells. 2. Regulation of bcl-2 gene expression in breast cancer cells. 3. Predictive markers of response to chemotherapy in breast and colon cancer, through screening of materials from NSABP clinical trials (several thousand cases) Facilities: The NSABP Pathology Section has over 10,000 cases of breast cancer and over 5,000 cases of colon cancer paraffin blocks with clinical follow-up in its central bank. The new 1000 square foot lab space will have its own tissue processor, microtomes, SAMBA 4000 image analyzer, fluorescent microscope with CGH software, automated immunostainer (Biotek techmate 500), Coulter Elite Flow cytometer, Laser Scissors, DNA sequencer, and other essential equipments for molecular biology. How to respond: Please e-mail, FAX, or mail me your CV, publication list, and brief description about yourself. Soon Paik, M.D. NSABP Pathology 320 E. North Ave. Pittsburgh, PA 15212 e-mail: spaik@asri.edu FAX: 412-359-8685