From owner-chromosomes@net.bio.net Sat Feb 01 22:00:00 1997 Path: biosci!maclan.mcgill.ca!thomas_bureau From: thomas_bureau@maclan.mcgill.ca (Thomas Bureau) Newsgroups: bionet.genome.chromosomes Subject: Two Post-Doc Positions Date: 1 Feb 1997 17:39:50 -0800 Organization: McGill University, Department of Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <32F3F0A1.21A4@maclan.mcgill.ca> Reply-To: 1205@sirocco.CC.McGill.CA, Dr.@sirocco.CC.McGill.CA, Penfield@sirocco.CC.McGill.CA, Avenue@sirocco.CC.McGill.CA, Montreal@sirocco.CC.McGill.CA, QC@sirocco.CC.McGill.CA, H3A@sirocco.CC.McGill.CA, 1B1@sirocco.CC.McGill.CA NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITIONS AVAILABLE Two postdoctoral positions are available immediately to work on the identification and characterization of transposable elements associated with wild-type plant genes. Many plant genes are associated with members of an unusual superfamily of transposable elements called Miniature Inverted-repeat Transposable Elements or MITEs. Possible projects include investigating the role of MITEs and other transposable elements in gene and genome evolution, characterizing the mechanism of MITE mobility, and using MITEs and other transposable elements in designing novel plant genome mapping and gene isolation protocols [Plant Cell 6, 907-916 (1994); PNAS 93, 8524 (1996)]. Candidates must be highly motivated and have a strong background in molecular biology and/or molecular genetics. Expertise in genome mapping or computational biology is desirable but not a prerequisite. Every effort will be made to facilitate the development of future careers in academia or industry. Please send CV, a brief statement of research goals, and the names of three referees to: Dr. Thomas E. Bureau McGill University Department of Biology 1205 Dr. Penfield Avenue Montreal, Quebec H3A 1B1 Canada. Email: thomas_bureau@maclan.mcgill.ca. Montreal is located in the Canadian province of Quebec. It is the second largest French speaking city in the world with Paris, France being the largest. Despite the strong French presence, Montreal is a multicultural multiethnic community. As such there is a great diversity of cultures, festivals, shops, and restaurants. Montreal also provides easy access to wilderness areas in eastern Canada and the northeast US. Housing is very reasonable with an average two bedroom apartment renting for approximately US$500/month. McGill University is a world class institution and one of the top universities in Canada. The Department of Biology is well rounded with faculty specializing in areas of ecology, evolutionary biology, molecular biology, genetics, developmental biology, cell biology, and neurobiology. Plant research is also well supported with a modern Phytotron facility and easy access to experimental field stations. From owner-chromosomes@net.bio.net Tue Feb 04 22:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!newspump.sol.net!howland.erols.net!rill.news.pipex.net!pipex!news.sprintlink.net!news-stk-200.sprintlink.net!news.aloha.net!news-w.ans.net!newsfeeds.ans.net!lantana.singnet.com.sg!usenet From: "4Ts" Newsgroups: bionet.genome.chromosomes Subject: functions of histones?? Date: 5 Feb 1997 11:19:49 GMT Organization: Singapore Telecom Internet Service Lines: 3 Message-ID: <01bc1356$80e77960$LocalHost@default> NNTP-Posting-Host: ts900-6719.singnet.com.sg X-Newsreader: Microsoft Internet News 4.70.1155 anyone know the function of the histones proteins other than sticking the DNA together to form chromosome... From owner-chromosomes@net.bio.net Thu Feb 06 22:00:00 1997 Path: biosci!rutgers!gatech!www.nntp.primenet.com!nntp.primenet.com!enews.sgi.com!insync!uunet!in1.uu.net!194.179.1.100!minerva.ibernet.es!diana.ibernet.es!pc068.isid.es From: Computer Contact-Carlos Enrile Newsgroups: bionet.genome.chromosomes Subject: Jornadas Genoma y Etica Date: Fri, 07 Feb 1997 01:46:27 +0100 Organization: Unisource Espana NEWS SERVER Lines: 6 Message-ID: <32FA7B63.28CB@isid.es> Reply-To: ccontact@isid.es NNTP-Posting-Host: asia.isid.es Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.01 (Win95; I) Jornadas de Reflexión sobre el Genoma Humano Fundacion Ramon Areces Madrid, Febrero de 1997 Mas información en: http://ourworld.compuserve.com/homepages/computercontact/conghom.htm From owner-chromosomes@net.bio.net Sat Feb 08 22:00:00 1997 Path: biosci!ECOCLUB.NSU.RU!funtik From: funtik@ECOCLUB.NSU.RU (Eugene Berezikov) Newsgroups: bionet.genome.chromosomes Subject: Collaboration offered: non-LTR retrotransposones study Date: 9 Feb 1997 14:34:39 -0800 Organization: Novosibirsk State University Lines: 106 Sender: daemon@net.bio.net Distribution: world Message-ID: <32F62C74.7F7D@ecoclub.nsu.ru> Reply-To: cellbiol@cgi.nsk.su NNTP-Posting-Host: net.bio.net Dr. Alexander Blinov Head of Cell Biology Laboratory Institute of Cytology and Genetics prospect Lavrentjeva, 10 Novosibirsk 630090 Russia Fax (3832) 35-65-58 Phone: (3832) 35-46-70 Email: cellbiol@cgi.nsk.su We are looking forward to establish collaboration with scientists interested in study of regulation of expression and transposition of non-LTR retrotransposons. As a first step we would like to get a JOINT GRANT with somebody interested in such kind of investigations. Here is a first draft of a project we propose: EXPRESSION OF THE NLRCTH1 ON THE DIFFERENT DEVELOPMENTAL STAGES AND UNDER DIFFERENT PHYSIOLOGICAL CONDITIONS. We have previously described and characterized two non-LTR rotransposons >From the Diptera Chironomus thummi (Blinov et al., 1993) and C.tentans (Blinov v et al., 1997). Also we have shown that the distribution of this elements is restricted by Chironomus genus. It has been shown that mininimum of three different non-LTR retrotransposons are present in the Chironomus genus. All of them contain similar nucleotide sequences in the region of the ORF2 which encodes reverse transcriptase. We disignated the retrotransposon as NLRCth1. It has been shown that a transposition of non-LTR retrotransposons causes haemophilia and adenocarcinoma in man (Kazazian et al, 1988; Morse et al, 1988), and hybrid dysgenesis in drosophila (Finnegan,1989). Expression of these elements is particularly high in certain embryonal carcinoma and teratocarcinoma cell lines (Leibold et al, 1990). It leads to speculation that expression and transposition of non-LTR retrotransposons may occur only in certain developmental stages. Moreover, the synthesis of full-length transcripts has been shown only for several organisms. We would like to investigate expression of the NLRCth1 on the different larval stages of Chironomus thummi using the Nothern blot analysis. The Chironomus is a very good subject for such kind of investigation. These animals have several very good separated developmental stages. Also, it will be interesting to show the influence of some kinds of stress on the expression of this element since the role of environmental conditions in transposition has been suggested. Heat stress, for example, can be used as an inductor for this purpose. Some transcription factors have been described wich bind specifically to non-LTR retrotransposon promoter region (Mathias and Scott, 1993). Such proteins are candidates for factors controlling expression of retrotransposons. Gene mobility shift assays may be used for investigations of differential expression of NLRCth1. The next experimental procedures in this project include: 1. Isolation of nuclear extracts from different stages of Chirnomus thummi. 2. Constructing hybrid plasmids containing different parts of NLRCth1 promoter region. 3. Gel mobility shift assays analysis, using the nuclear extracts and DNA probes. 4. DNAse I protection assays. 5. Constructing of Chironomus thummi expression library. 6. Screening of this library using South-West analysis. 7. Isolation and characterization of positive clones. Using this approach we hope to get answers for two important questions: 1. Is the expression of non-LTR retrotransposons stage- and tissue- specific; and whether physiological conditions influence their expression or not. 2. Whether transcription factors which may control expression of the NLRCth1 present or not. REFERENCES Blinov A.G., Sobanov Y.V., Bogachev S.S., Donchenko A.P., Filippova M.A. (1993) The Chironomus thummi genome contains a non-LTR retrotransposon. Mol Gen Genet 237: 412-420. Blinov A.G., Sobanov Y.V, Scherbik S.V. and Aimanova K.G. (1997) The Chironomus (Camptochironomus) tentans genome contains two non-LTR retrotransposons. Genome, in press. Finnegan D.J. (1989) Eucariotic transposable elements and genome evolution. Trends in Genet 5: 103-107. Kazazian H.H., Wong C., Youssufian H., Scott A.F., Philips D.G., Antonarakis S.E. (1988) Haemophilia A resulting from de novo insertion of L1 sequences represents a novel mechanism for mutation in man. Nature 332: 164-166. Leibold D.M., Swergold G.D., Singer M.F., Thayer B.A., Dombroski ,B.A., Fanning T.G. (1990) Translation of LINE-1 elements in vitro and in human cells. Proc Natl Acad Sci USA 87: 6990-6994. Mathias S.L., Scott A.F. (1993) Promoter binding proteins of an active human L1 retrotransposon. Bioch Bioph Res Commun 191: 625-632. Morse B., Rotherg P.G., South V.J., Spandorfer J.M., Astrin S.M. (1988) Insertional mutagenesis of the myc locus by a LINE-1 sequence in human breast carcinoma. Nature 333: 87-89. From owner-chromosomes@net.bio.net Sat Feb 15 22:00:00 1997 Path: biosci!USERS.AFRICAONLINE.CO.KE!wellcome From: wellcome@USERS.AFRICAONLINE.CO.KE ("Wellcome Trust Research Labs, Nairobi") Newsgroups: bionet.genome.chromosomes Subject: help/help Date: 16 Feb 1997 07:40:55 -0800 Organization: Wellcome Trust Research Labs, Nairobi Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <330782B2.54D4@users.africaonline.co.ke> NNTP-Posting-Host: net.bio.net To the group I am analysing point mutation dihydroreductase gene of P. Falciparum. This gene encodes for the protein target of Pyrimethamine, the drug used against this parasites. It is known that point mutation are linked the decrease of Pyr effectiveness on isolates. Mainly, there are 3 point mutations: one prerequisite, at codon 108 two others occurring independently or together, always with the mutation at codon 108. My question is: Can we assess the order in with the others mutations appear, based on their frequencies? If yes, which test to use and the related references. My regards to all. Dr. Alexis Nzila: Wellcome@users.africaonline.co.ke (Specify, message for Alexis Nzila). From owner-chromosomes@net.bio.net Sat Feb 15 22:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!newsfeeds.sol.net!news.maxwell.syr.edu!cam-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!howland.erols.net!rill.news.pipex.net!pipex!oleane!pasteur.fr!jussieu.fr!infobiogen.fr!sansgene.genethon.fr!not-for-mail From: bernot@sansgene.genethon.fr (Alain Bernot) Newsgroups: bionet.genome.chromosomes Subject: interspersed sequences Date: 16 Feb 1997 21:01:36 +0100 Organization: Genethon -- Human Genome Research Centre Lines: 20 Message-ID: <5e7p30$3eu@sansgene.genethon.fr> NNTP-Posting-Host: sansgene.genethon.fr hi can anybody tell me if the interspersed sequences in the murine genome are related to a known gene (in the same way as the human Alu is related to the 7SL RNA in primates). I am also looking for articles about repeated sequences in non-human species. thanks _______________________________________________________________________________ Alain Bernot | Phone : +33-1-69-47-25-50 | Genethon | Fax : +33-1-60-77-86-98 | Human Genome Research Center | e-mail: bernot@genethon.fr | 1, Rue de l'Internationale BP 60 | http://www.genethon.fr | 91002 Evry, France | | _______________________________________________________________________________ From owner-chromosomes@net.bio.net Sun Feb 16 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!news.sgi.com!howland.erols.net!math.ohio-state.edu!jussieu.fr!univ-lyon1.fr!news From: duret@biomserv.univ-lyon1.fr (Laurent Duret) Newsgroups: bionet.genome.chromosomes,bionet.molbio.evolution Subject: Re: interspersed sequences Date: 17 Feb 1997 08:37:13 GMT Organization: Universite Claude Bernard - Lyon 1 Lines: 32 Message-ID: <5e95bp$1lh@tempo.univ-lyon1.fr> References: <5e7p30$3eu@sansgene.genethon.fr> Reply-To: duret@biomserv.univ-lyon1.fr NNTP-Posting-Host: biomserv.univ-lyon1.fr Xref: biosci bionet.genome.chromosomes:1538 bionet.molbio.evolution:5676 In article <5e7p30$3eu@sansgene.genethon.fr>, bernot@sansgene.genethon.fr (Alain Bernot) writes: > > >hi > >can anybody tell me if the interspersed sequences in the murine genome are >related to a known gene (in the same way as the human Alu is related to the 7SL >RNA in primates). I am also looking for articles about repeated sequences in >non-human species. > Yes, if I remember, B1 repeats in rodents also derive from the 7SL RNA gene. Indeed most of SINES in vertebrates derive from small RNA genes (tRNA, 7SL, ...). For a review, look at Deininger at al. 1989 In: Berg & Howe (ed) Mobile DNA, American Society for Microbiology, Washington DC, pp.619-636. Hope this helps, __________________________________________________________________________ Laurent Duret Laboratoire BGBP - UMR CNRS 5558 Phone : +33 472 44 80 00 p.34 39 Universite Claude Bernard - Lyon 1 FAX : +33 478 89 27 19 43 Bd du 11 Novembre 1918 e-mail : duret@biomserv.univ-lyon1.fr F-69622 Villeurbanne Cedex ======================== France __________________________________________________________________________ From owner-chromosomes@net.bio.net Tue Feb 18 22:00:00 1997 Path: biosci!agate!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!uunet!in1.uu.net!140.142.64.3!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes,bionet.molbio.evolution Subject: Re: interspersed sequences Date: 19 Feb 1997 00:58:35 GMT Organization: University of Washington, Seattle Lines: 15 Message-ID: <5edj7r$ddu@nntp3.u.washington.edu> References: <5e7p30$3eu@sansgene.genethon.fr> <5e95bp$1lh@tempo.univ-lyon1.fr> NNTP-Posting-Host: saul4.u.washington.edu NNTP-Posting-User: roach Xref: biosci bionet.genome.chromosomes:1539 bionet.molbio.evolution:5683 A literature search for the author "Arian Smit" should be productive. ------------------------------------------------------------------ Jared C. Roach Department of Molecular Biotechnology Health Sciences Building, Room K354 University of Washington Box 357730 Seattle, WA 98195 phone (206) 616-4536 FAX (206) 685-7301 roach@u.washington.edu http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Mon Feb 24 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-xfer.netaxs.com!ddsw1!news.mcs.net!van-bc!unixg.ubc.ca!enswartz From: enswartz@unixg.ubc.ca (Erik Nathan Swartz) Newsgroups: bionet.genome.chromosomes Subject: Microdeletion question Date: 25 Feb 1997 01:19:46 GMT Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 21 Message-ID: <5eteni$eu4$1@nntp.ucs.ubc.ca> NNTP-Posting-Host: netinfo1.ubc.ca X-Newsreader: TIN [version 1.2 PL2] I got the question in a problem set in my medical genetics course (2nd year medicine), and I was wondering if I'm on the right track with the answer: -23 yr old women with mental retardation and unusual appearance is 10 wks pregnant -FISH testing shows her to have a microdeletion on the proximal arm of chromosome 7 -negative family history -partner is healthy with chromosomal analysis of 46, XY -What is the risk of recurrence of this woman's condition in her child? I would say 50%, but I feel I may be missing some important point. Thanks in advance for your help. -- Erik Swartz UBC Medicine Vancouver, Canada From owner-chromosomes@net.bio.net Mon Feb 24 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!uunet!in2.uu.net!198.151.175.33!news.xensei.com!news From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.genome.chromosomes Subject: Bioinformatics and Genome Research Conference Date: Tue, 25 Feb 1997 12:19:36 GMT Organization: Cambridge Healthtech Institute Lines: 94 Message-ID: <5eul7k$mdg@xensei3.xensei.com> Reply-To: chi@healthtech.com NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 Cambridge Healthtech Institute's Sixth International Bioinformatics and Genome Research Conference June 11-12, 1997 Fairmont Hotel, San Francisco, CA Scientific Advisory Committee Dr. Keith O. Elliston, Bayer Corporation Dr. Kenneth H. Fasman, Whitehead Institute of Genomic Research Dr. Nathan Goodman, Jackson Laboratory Dr. Hwa A. Lim, Pangea Systems, Inc. Dr. G. Christian Overton, University of Pennsylvania Co-sponsoring Journal: GENE Emerging Technologies Dr. Temple Smith, Boston University (tentative) Complex Software Systems with Low-Energy Evolution and Maintenance: Dr. Otto Ritter, German Cancer Research Center Integrating Data and Software for Genome Discovery: Dr. Tod Klingler, Incyte Pharmaceuticals DNA Probe Arrays- Accessing the Genome: Dr. Robert J. Lipshutz, Affymetrix, Inc. Bioinformatics for Quantitative Expression Analysisä and GeneScapeä: Dr. Aleksandar Milosavljevic, CuraGen Corporation An Operating System for Drug Discovery: Dr. Hwa A. Lim, Pangea Systems, Inc. Functional Analysis of Genes Bioinformatics and the Challenge of Functional Genomics: Dr. G. Christian Overton, University of Pennsylvania Homology Protein Structure Modeling in Genome Research: Dr. Andrej Sali, Rockefeller University From Sequence to Structure, Function & Therapeutic Targets: Dr. Kevin Ulmer, SeQ Technology Function of E. Coli Proteins: Dr. Monica Riley, Marine Biological Laboratory Identification of Distantly Related Proteins from Database Searches: Dr. Patricia C. Babbitt, University of California, San Francisco Protein Structure Prediction: Towards Automatic Modeling of Function from Sequence: Dr. Michael Levitt, Molecular Applications Group (confirmed) New Data Tools Using the Human Genome Database to Find Candidate Genes: Dr. Kenneth H. Fasman, Genome Database Integrating Human Maps in Genome Data Base: Dr. Stanley Letovsky, Genome Data Base Federating and Exploring Molecular Biology Databases: A Tool Based Approach: Dr. Victor M. Markowitz, Lawrence Berkeley National Laboratory Object-Oriented Knowledge Base Management Tools for Bioinformatics: Dr. Peter Karp, SRI International Integrating ABI Prism(r) DNA Sequencers with a Relational Database: Dr. Timothy Burcham, PE Applied Biosystems Division (confirmed) Pharmaceutical Lead Discovery Linking the Technologies of Early Lead Discovery Through Informatics: Dr. Keith O. Elliston, Bayer Corporation Applying a Bioinformatics Approach to Molecular Disease Management: Dr. Michael N. Liebman, Vysis, Inc. Capturing Lead Target Genes: Combining High Throughput Screening and Bioinformatics Approaches: Dr. Tauseef R. Butt, Gene Transcription Technologies, Inc. And University of Pennsylvania How Can Genetics and Bioinformatics Help in Pharmaceutical Product Development: Dr. Rainer Fuchs, Glaxo Wellcome Research and Development A Team-Centric, Database Approach to Using Genome Information for Pharmaceutical Discovery: Dr. Martin Sumner-Smith, Base4 Bioinformatics Inc. For registration and hotel information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-chromosomes@net.bio.net Tue Feb 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!zephyr.texoma.net!news.stealth.net!cdc2.cdc.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.sprintlink.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news.sprintlink.net!news-stk-3.sprintlink.net!news.rain.net!hevanet.com!usenet From: Fiona Dent Newsgroups: bionet.genome.chromosomes Subject: New Scientist Has Latest on Cloning Date: Wed, 26 Feb 1997 13:55:24 -0800 Organization: New Scientist Planet Science Lines: 26 Message-ID: <3314B14C.2E2@ipc.co.uk> Reply-To: Fiona_Dent@ipc.co.uk NNTP-Posting-Host: br-ppp03.hevanet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) New Scientist Has Latest on Cloning LONDON, ENGLAND -- February 26, 1997 (INB) -- The leading weekly science and technology news magazine, New Scientist, is launching a new section on its web site, New Scientist Planet Science (http://www.newscientist.com/) about the cloning of an adult sheep and its implications. The Cloning - Special Report Section at http://www.newscientist.com/clone/ will be published 18:00 GMT (13:00 EST) on February 26th and will contain the following and more: --This week's news stories from New Scientist and the editorial --Relevant web links --Letters and e-mail about the issue as they come in to our magazine and the web site --The New Scientist Planet Science Ethics Forum where we will ask our visitors whether there are any circumstances in which they believe human cloning could be ethical. Your site could be one of the ones we will be linking to. We would appreciate it if you could let your readers know about this section. Please let us know if you do so. # # # From owner-chromosomes@net.bio.net Wed Feb 26 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!news.sgi.com!howland.erols.net!newsxfer.itd.umich.edu!uunet!in3.uu.net!198.151.175.33!news.xensei.com!news From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.genome.chromosomes Subject: Cambridge Healthtech Institute’s MOLECULAR EVOLUTION Date: Thu, 27 Feb 1997 18:14:08 GMT Organization: Cambridge Healthtech Institute Lines: 129 Message-ID: <5f4io7$qqc@xensei3.xensei.com> Reply-To: chi@healthtech.com NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 Cambridge Healthtech Institute’s MOLECULAR EVOLUTION April 24-25, 1997 Copley Plaza, Boston, Massachusetts Co-sponsored by: Canji, Inc., Pharmacia Biotech, and PharmaGenics A large number of biomedical applications involve the search for compounds with improved activity or performance. The application of molecular evolution is proving to be extremely well suited for this purpose, particular for identification of nucleic acids or peptides. Genetics makes it possible to employ sequential rounds of screening and selection, followed by further mutation, to allow for the generation of compounds adapted for a desired target or under specific conditions. Phage display and other related techniques have been used extensively for this purpose. Examples of the development of antibodies, mutated proteins, and a range of research, selection, and therapeutic applications are covered in detail. Learn how leaders in this field are taking advantage of the power of this technology and how you might benefit from making greater use of it by attending this timely event. NUCLEIC ACID EVOLUTION In Vivo and In Vitro Applications of Nucleic Acid Ligands Dr. Daniel W. Drolet, NeXstar Pharmaceuticals (confirmed) Mirror-Image RNA Ligands Dr. Jens P. Furste, Freie Universitst Berlin (confirmed) Aptamer Evolution: Prospects for Diagnostics and Therapeutics Dr. Andrew D. Ellington, Indiana University (confirmed) Ribozymes from Random Sequences: Dr. David Bartel, Whitehead Institute and Massachusetts Institute of Technology (confirmed) ENGINEERING PROTEINS AND ANTIBODIES Exploiting the Diversity of Large Human Antibody Libraries Dr. John McCafferty, Cambridge Antibody Technology Ltd. (confirmed) Molecular Evolution of Proteins, Pathways and Viruses by DNA Shuffling Dr. Willem Stemmer, Maxygen, Inc. (confirmed) In Vitro Selection of Peptides Acting on NMDA Glutamate Receptors Dr. Min Li, John Hopkins School of Medicine (confirmed) Synthesis Based Molecular Design of Antibodies and Proteins: Dr. William D. Huse, Ixsys, Inc. (confirmed) Dr. Manuel Baca, Genentech, Inc. (confirmed) RESEARCH AND SELECTION USING PHAGE DISLAY Cloning Novel Genes with Phage-Displayed Peptide Ligands Dr. Brian Kay, University of North Carolina at Chapel Hill (confirmed) Adenovirus Expression Systems in Conjunction with Autofluorescent Proteins: Potential Use in the Screening of Peptide Libraries Dr. Luc Peloquin, Quantum Biotechnologies Inc. (confirmed) Functional Epitope Mapping by Negative Selection of Phage-Displayed Randomized Protein Libraries Dr. Laurent Jespers, Flanders Interuniversity Institute for Biotechnology (confirmed) Automated Affinity Based Screening Using Serial Column Chromatography Interfaced with Mass Spectrometry Dr. Satish Jindal, ChemGenics Pharmaceuticals (confirmed) Use of Combinatorially Generated Phage Displayed Ligands to Develop Engineered Microprotein Ligands for Large Scale Biotherapeutic Purification Dr. John Maclennan, Dyax Corporation (confirmed) Identification of Optimal Ligands for Protein Domains Using Oriented Peptide Libraries Dr. Zhou (Sonny) Songyang, Harvard Medical School (confirmed) THERAPEUTIC DEVELOPMENTS USING PHAGE DISPLAY Identification of Amino Acids Critical for the Activity of a Peptide Mimetic of Erythropoietin and Discription of a Minimal Functional Epitope Dr. Dana Johnson, R.W. Johnson Pharmaceutical Research Institute (confirmed) Combinatorial Methods and Directed Evolution Applied to Staphylococcal a-hemolysin, a Pore-Forming Protein Dr. Hagan Bayley, Worcester Foundation for Biomedical Research (confirmed) Human Protein Display: A New System for Discovering Drug Binding Targets Dr. Allan Peng, GeneMax, Inc. (confirmed) Novel Approaches for Targeting Tumors and Inhibiting Metastasis Dr. Renata Pasqualini, La Jolla Cancer Research Center, The Burnham Institute (confirmed) Sampling Peptide Space for Cell-Targeting Peptides Dr. Stephen A. Johnston, University of Texas, Southwestern Medical Center, (confirmed) Cloning Using Phage Display Dr. Bob Shopes, Tera Biotechnology Corporation (confirmed) Establishing Site Directed Assays Using Phage Displayed Libraries of Peptides and Antibodies Dr. Arthur J. Blume, DGI BioTechnologies, L.L.C. (confirmed) From the rapid progess of combinatorial chemistry and genetics an interest has been generated in the selection of random peptides from target proteins and in investigating ligand-receptor interactions. Some challenges that will be examined are the screening of combinatorial libraries for selecting high-affinity ligands against target molecules, following the sequential cycles of random mutagenesis and screening to direct the evolution of enzymes, and developing new techniques such as phage display for optimum peptide /receptor binding. The expectation that from the selection and use of phage display, proteins for therapeutics and drug discovery may be developed. Advance registration March 7, 1997 poster deadline March 21, 1997 For registration and hotel information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-chromosomes@net.bio.net Wed Feb 26 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Feb 1997 02:00:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702271000.CAA05027@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net