From owner-chromosomes@net.bio.net Sun Mar 02 22:00:00 1997 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news.rediris.es!news.uoregon.edu!hammer.uoregon.edu!news-xfer.netaxs.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!newsxfer.itd.umich.edu!uunet!in3.uu.net!208.202.120.50!server2.cognit.com!sara From: sara@cognit.com (Sara) Newsgroups: bionet.genome.chromosomes Subject: Quick Query: Synthetic meiosis from DNA, relating to fertility where a man has no testicles. Date: Mon, 03 Mar 1997 21:49:49 GMT Organization: Cognitive Communications Lines: 33 Message-ID: <5ff6am$gos@server2.cognit.com> NNTP-Posting-Host: 208.202.120.59 X-Newsreader: Forte Agent .99c/32.126 Before I share the actual post, I would like to get a few things taken care of. Sorry in advance for the crosspost, but as I have not posted in these groups or relating to this matter, I could not decide which group(s) to include. If someone feels this is not the group where this should be posted, or if they wish to suggest a better group, please feel free to email me. Next I would like to address the fact that I am not a biologist. I have a scientific background, and some biology, but my understanding of certain biological concepts is far from perfect, or current. Keeping this in mind, please read on. Subject: The status of biology relating to synthetic meiosis from DNA. What I am looking for is someone with a lot of knowledge about current genetics, and biology to answer the following for me. I am in a relationship with a man whose child I desire to have. The problem is that he had a accident during youth resulting in the loss of his testicles. I am looking for a way to have his child anyway. The only thing I could come up with is some type of artificial meiosis from his DNA, to produce the genetic information needed to replace the genetic information in a donor sperm, which could then be implanted in the egg resulting in fertilization. Since I have a limited amount of information on this subject, I would appreciate any bits you can give me relating to this subject. Can science already do this? If not then what piece is missing? Etc. Or perhaps we can start a conversation on this subject. I believe this could help a lot of people out there. Thanks for reading.. Sara Eve Posner From owner-chromosomes@net.bio.net Sun Mar 02 22:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!dispatch.news.demon.net!demon!uknet!usenet1.news.uk.psi.net!uknet!EU.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!newsxfer.itd.umich.edu!uunet!in3.uu.net!208.202.120.50!server2.cognit.com!sara From: sara@cognit.com (Sara) Newsgroups: bionet.genome.chromosomes Subject: Quick Query: Synthetic meiosis from DNA, relating to fertility where a man has no testicles. Date: Mon, 03 Mar 1997 21:48:50 GMT Organization: Cognitive Communications Message-ID: <5ff68r$gos@server2.cognit.com> NNTP-Posting-Host: 208.202.120.59 X-Newsreader: Forte Agent .99c/32.126 Lines: 33 Before I share the actual post, I would like to get a few things taken care of. Sorry in advance for the crosspost, but as I have not posted in these groups or relating to this matter, I could not decide which group(s) to include. If someone feels this is not the group where this should be posted, or if they wish to suggest a better group, please feel free to email me. Next I would like to address the fact that I am not a biologist. I have a scientific background, and some biology, but my understanding of certain biological concepts is far from perfect, or current. Keeping this in mind, please read on. Subject: The status of biology relating to synthetic meiosis from DNA. What I am looking for is someone with a lot of knowledge about current genetics, and biology to answer the following for me. I am in a relationship with a man whose child I desire to have. The problem is that he had a accident during youth resulting in the loss of his testicles. I am looking for a way to have his child anyway. The only thing I could come up with is some type of artificial meiosis from his DNA, to produce the genetic information needed to replace the genetic information in a donor sperm, which could then be implanted in the egg resulting in fertilization. Since I have a limited amount of information on this subject, I would appreciate any bits you can give me relating to this subject. Can science already do this? If not then what piece is missing? Etc. Or perhaps we can start a conversation on this subject. I believe this could help a lot of people out there. Thanks for reading.. Sara Eve Posner From owner-chromosomes@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!EU.net!news2.EUnet.fr!vodka.cephb.fr!not-for-mail From: Mourad Sahbatou Newsgroups: bionet.general,bionet.genome.chromosomes,bionet.molbio.gene-linkage,bionet.software Subject: THE CEPH GENOTYPE DATABASE V8.1 AVAILABLE Date: Thu, 06 Mar 1997 17:53:47 +0100 Organization: Fondation J. DAUSSET - C. E. P. H. Lines: 33 Message-ID: <331EF69B.4ED0@cephb.fr> NNTP-Posting-Host: vodka.cephb.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; SunOS 5.4 sun4d) CC: mourad.sahbatou@cephb.fr Xref: biosci bionet.general:25916 bionet.genome.chromosomes:1553 bionet.molbio.gene-linkage:1344 bionet.software:18066 THE CEPH GENOTYPE DATABASE V8.1 AVAILABLE ON WEB AND FTP SERVERS. The most recent version of The CEPH genotype database (V8.1) is now available to the scientific international community. The database holds over 2,530,000 CEPH family genotypes generated during the last 13 years for some 11,932 polymorphic markers, including more than 8,900 microsatellite markers ( 57% of which are hightly polymorphic). The mean heterozygote frequency of the loci in V8.1 is 0.64, and the mean number of alleles per locus is 6.2, essentially reflecting the large number of microsatellites in the database. In addition to the raw genotypes, the database provides users with linkage file formats of markers and two-point lod scores among all pairs of markers on the same chromosome (3,544,000 two-point lodscores have been computed). You can efficiently browse and dump the data from our Web/database interface (http://www.cephb.fr/cephdb/). Dump programs allow you to dump data for several markers on the same chromosome in CEPH, LINKAGE and CRIMAP formats. CEPH anonymous FTP server : ftp://ftp.cephb.fr/pub/ceph_genotype_db/ CEPH genotype database Web server : http://www.cephb.fr/cephdb/ CEPH World Wide Web home page : http://www.cephb.fr CEPH database manager : cephdbm@cephb.fr ---------------------------------------------------------------------- Mourad SAHBATOU Fondation Jean DAUSSET - CEPH CEPH database manager 27, rue Juliette Dodu cephdbm@cephb.fr 75010 Paris, France From owner-chromosomes@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!MAILHOST.TCS.TULANE.EDU!alustig From: alustig@MAILHOST.TCS.TULANE.EDU ("Lustig, Arthur J.") Newsgroups: bionet.genome.chromosomes Subject: telomeres at Tulane Date: 10 Mar 1997 06:14:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Postdoctoral position to study telomere structure and function. A postdoctoral position is available to investigate telomere dynamics in Saccharomyces cerevisiae in the laboratory of Art Lustig at Tulane University Medical Center. We have had a long-standing fascination in telomere structure and function and are currently focusing on two broad areas: 1) The mechanisms of nucleation and inheritance of telomeric silencing (e.g. MCB 16: 2843); and 2) the mechanism of telomere size maintainence (e.g. Genes Dev. 10: 1310). Candidates should have a background in molecular biology and/or genetics. Background in yeast is preferable but not required. Interested candidates should send their c.v. either by e-mail, fax, or mail to the address listed below. Arthur J. Lustig Associate Professor Department of Biochemistry SL43 Tulane University Medical Center 1430 Tulane Avenue New Orleans, LA 70112 phone: 504-584-3688 fax: 504-584-2739 From owner-chromosomes@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!GENOME.WI.MIT.EDU!slonim From: slonim@GENOME.WI.MIT.EDU (Donna Slonim) Newsgroups: bionet.genome.chromosomes Subject: ANNOUNCEMENT: Whitehead/MIT Mouse Physical Map Data Release Date: 10 Mar 1997 13:01:36 -0800 Organization: Whitehead Institute for Biological Research Lines: 179 Sender: daemon@net.bio.net Distribution: world Message-ID: <332476E4.2781@genome.wi.mit.edu> NNTP-Posting-Host: net.bio.net ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH MOUSE GENOMIC MAPPING PROJECT DATA RELEASE 13 (MARCH 1997) (available at http://www-genome.wi.mit.edu/cgi-bin/mouse/index) ---------------------------------------------------------------------------- Genetic Map The July 1996 Data Release was the final release of the Mouse Genetic Map at the Whitehead Institute/MIT Center for Genome Research. It reflects the data represented in the 14 March, 1996 issue of Nature. Please see this paper, and also Genetics 131:423-447 (1992) for descriptions of the materials and methods used to construct the maps. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. ---------------------------------------------------------------------------- Mouse Physical Mapping Project Having completed the genetic map, we are now constructing a physical map of the mouse consisting of 10,000 markers screened against a mouse YAC library. Many of the markers are the SSLPs that are also on the genetic map, but are also adding random STSs to the physical map to reach a total of 10,000 markers. In this release, we include data for 6,018 markers successfully screened against the YAC library. We have placed 4,917 of these markers into singly-linked physical map contigs; 4,191 are in doubly-linked contigs. The table below shows the number of physically and genetically-mapped markers assigned to each chromosome. (Note that some markers that hit yacs have not yet been mapped to a particular chromosome, so the total number of physically-mapped markers in this table represents a subset of the total number of markers in the release.) The right-most columns count the number of markers shared by the two maps ("BOTH"), and the total number of distinct markers mapped in any way ("TOTAL MARKERS"). Breakdown of Mapped Markers by Chromosome CHROMOSOME PHYSICAL GENETIC BOTH TOTAL MARKERS Chr1 431 559 403 655 Chr2 399 553 388 656 Chr3 250 382 229 458 Chr4 261 367 240 430 Chr5 288 433 268 511 Chr6 294 383 252 482 Chr7 272 356 260 423 Chr8 263 361 262 404 Chr9 256 371 235 444 Chr10 243 317 223 389 Chr11 324 356 280 470 Chr12 241 289 222 335 Chr13 281 317 266 376 Chr14 216 271 202 327 Chr15 238 274 215 346 Chr16 202 215 172 267 Chr17 127 255 107 312 Chr18 192 236 155 306 Chr19 125 134 113 169 ChrX 179 230 153 287 TOTAL 5082 6659 4645 8047 YAC Library The YAC library being used is described in an upcoming issue of Mammalian Genome (Haldi, et al.). It consists of 40,000 YACs with an average insert size of 820 kb. The entire library, or individual YAC clones, is available through Research Genetics, Huntsville, Alabama (phone, 800-533-4363; outside US/Canada, 205-533-4363; FAX, 205-536-9016) and Genome Systems, Inc. of St. Louis, Missouri, (phone 800-430-0030). We are currently using a subset of 24,000 clones in building the physical map. These 24,000 clones are arrayed here at the Center in a 5-dimensional pooling scheme. The STSs are screened on these 5D pools, with 3 hits describing a single YAC address, and up to two extra hits confirming that address. STSs screened Please note that this release of the physical map represents a work in progress. It includes only those SSLPs that worked well on the first try and that did not require any special attention. There are now 5,080 SSLPs on the physical map, as well as 868 random STSs and 70 ESTs. In future releases, we expect to add primarily ESTs and random markers. The genetically-mapped SSLPs are screened on the 5-dimensional pools, using the same pair of primers as were used when we placed them on the genetic map. If the marker fails on the first try, it is run through the screening process a second time. If it fails twice, we attempt to convert the SSLP into an STS by picking two new primers and an internal oligo from one side or the other side of the CA-repeat. Markers that fail this final step will probably not be placed on the physical map. If you are working with an SSLP that you want to know the precise placement of in relation to other SSLPs nearby, you may wish to consult the European Collaborative Interpecific Mouse Backcross. They are mapping our SSLPs in a high-resolution 1000-animal backcross. Chromosomes 2, 11, 15, 16, and 17 are completed, chromosomes 3 and 4 are nearly done, and others are underway. Many of the markers on both the genetic and physical map are available through Research Genetics, at a reduced cost under a community discount arrangement set up by the WICGR. (Note: WI/MIT CGR has placed the markers in the public domain. The center and its personnel receive no financial benefit from the sale of primers.) Citing this data Data releases occur on a quarterly basis or more frequently if the amount of new data warrants it. At the end of each quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within two weeks of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy is among the most rapid, broad and regular of any genome center. Its purpose is to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. References to this data in publications should be cited by listing each of the following three sources: 1. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. 2. Dietrich, W.F. et al. (1994) A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genetics 7:220-245. 3. Copeland, N.G., D.J. Gilbert, N.A. Jenkins, J.H. Nadeau, J.T. Eppig, L.J. Maltais, J.C. Miller, W.F. Dietrich, R.G. Steen, S.E. Lincoln, A. Weaver, D.C. Joyce, M. Merchant, M. Wessel, H. Katz, L.D. Stein, M.P. Reeve, M.J. Daly, R.D. Dredge, A. Marquis, N. Goodman, E.S. Lander (1993) Genome Maps IV. Science 262:67. 4. Supplemented by additional markers in: Whitehead Institute/MIT Center for Genome Research, Genomic Map of the Mouse, Database Release 10, June 1996. Additional publications with related information: Dietrich, W., J. Miller, H. Katz, D. Joyce, R. Steen, S. Lincoln, M. Daly, M.P. Reeve, A. Weaver, P. Anagnostopoulos, N. Goodman, N. Dracopoli, E.S. Lander (1992) Genetic Maps. Stephen J. O'Brien, ed. Cold Spring Harbor Laboratory Press. Assay Conditions for the Genetic Map: Assay conditions and other experimental details are given in: Dietrich, W., et al., 1992. Genetics 131: 423-447. Briefly, the PCR protocol used radiolabeled primers in a 25 cycle PCR (1 '94 degrees, 2' 55 degrees, 3' 72 degrees) on 20 ng of genomic DNA. Contents of the release directory 3-97.MarkerInfo.txt PCR primer and product size 3-97.GeneticMapInfo.txt Genetic mapping information 3-97.PhysicalMapInfo.txt Physical mapping information 3-97.STS2Contig.txt Contig information 3-97.Contig2STS.txt Contig information - inverted 3-97.Sequences.txt Raw source sequences for STSs pictures/ Maps in postscript and Macintosh format ---------------------------------------------------------------------------- For further information contact: Victoria Wang victoria@genome.wi.mit.edu or Donna Slonim slonim@genome.wi.mit.edu Last modified March 10, 1997 From owner-chromosomes@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!howland.erols.net!portc02.blue.aol.com!newstf02.news.aol.com!audrey01.news.aol.com!not-for-mail From: bennybob16@aol.com (BennyBob16) Newsgroups: bionet.genome.chromosomes Subject: Re: Do you like Nude Celebrity Pic? How about Date: 12 Mar 1997 20:40:35 GMT Organization: AOL http://www.aol.com Lines: 1 Message-ID: <19970312204001.PAA16325@ladder01.news.aol.com> References: <5fefie$i16@argentina.earthlink.net> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Did you read the description of this news group you stupid shit?? From owner-chromosomes@net.bio.net Fri Mar 14 22:00:00 1997 Path: biosci!agate!spool.mu.edu!howland.erols.net!vixen.cso.uiuc.edu!milo.mcs.anl.gov!usenet From: pusch@mcs.anl.gov (Gordon D. Pusch) Newsgroups: bionet.genome.chromosomes Subject: RE: Re: Do you like Nude Celebrity Pic? How about Followup-To: alt.flame Date: 14 Mar 1997 22:35:05 -0600 Organization: Consulting computational physicist Lines: 15 Sender: pusch@juju.mcs.anl.gov Message-ID: References: <5fefie$i16@argentina.earthlink.net> <19970312204001.PAA16325@ladder01.news.aol.com> NNTP-Posting-Host: juju.mcs.anl.gov In-reply-to: bennybob16@aol.com's message of 12 Mar 1997 20:40:35 GMT CC: bennybob16@aol.com X-Newsreader: Gnus v5.1 In article <19970312204001.PAA16325@ladder01.news.aol.com> bennybob16@aol.com (BennyBob16) writes: > Did you read the description of this news group you stupid sh*t?? He/She/It Probably did not --- in fact, most likely the ad was posted by a Spam-bot. You'd do a =LOT= more good if you were to complain to his/her/its ISP, rather than inflicting your indignation on the rest of the group, however... :-T -- Gordon D. Pusch Disclaimer: I'm a consultant --- I don't speak for ANL or the DOE, and they *certainly* don't speak for =ME= !!! From owner-chromosomes@net.bio.net Sun Mar 23 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!agate!news.ucsc.edu!cbarrett From: cbarrett@cse.ucsc.edu (Christian Barrett) Newsgroups: bionet.genome.chromosomes Subject: Cannot find gene markers Date: 24 Mar 1997 20:31:34 GMT Organization: UC Santa Cruz CIS/CE Lines: 17 Message-ID: <5h6ob6$jl7@darkstar.ucsc.edu> NNTP-Posting-Host: sundance.cse.ucsc.edu In the Nov. 15, 1996 issue of SCIENCE, Polymeropoulos et al. have mapped the gene for Parkinson's disease to chromosome 4. In particular, "between markers D4S2361 and D4S421 at a recombination distance of 0.00 cM from marker D4S1647". I have only been able to locate marker D4S421. The other two don't seem to exist anywhere. I've tried Unigene, Genethon, and most of the other mapping sites on the web (like Sanger, Whitehead/MIT, Wellcome Trust -- even though they're not necessarily involved with Chromosome 4). Could somebody explain why all markers would not be available, some false assumptions I've made in expecting to find them, or point to a site that I can locate them? Thanks, Christian Barrett From owner-chromosomes@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.sprintlink.net!news-peer.sprintlink.net!EU.net!news2.EUnet.fr!vodka.cephb.fr!not-for-mail From: Denis Le-Paslier Newsgroups: bionet.genome.chromosomes Subject: (no subject) Date: Wed, 26 Mar 1997 11:17:34 +0100 Organization: ceph Lines: 28 Message-ID: <3338F7BE.A50@cephb.fr> NNTP-Posting-Host: vodka.cephb.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (X11; I; SunOS 5.4 sun4d) CC: cbarrett@cse.ucsc.edu Dear, D-numbers are given by GDB. Using the GDB www server (http://gdbWWW.gdb.org) you will find D4S11647 as a polymorphic marker from CHLC and you will be able to have the primer's sequences as well the location of this STS. It was present also in dbSTS, RHDB, CHLC, WICGR ... databases. It the same for D4S2361. yours, -- **** Denis Le Paslier Fondation Jean Dausset - CEPH Centre d'Etude du Polymorphisme Humain 75010 Paris France tel: [33](1) 53 72 51 15 fax: [33](1) 53 72 50 58 / 51 51 **** From owner-chromosomes@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Mar 1997 02:00:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703271000.CAA16009@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Fri Mar 28 22:00:00 1997 Path: biosci!GIG.USDA.GOV!srheller From: srheller@GIG.USDA.GOV ("Stephen R. Heller") Newsgroups: bionet.genome.chromosomes Subject: PAG-VI meeting Date: 29 Mar 1997 10:52:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The first preliminary draft for the PAG-VI meeting is available at: http://probe.nalusda.gov:8000/otherdocs/pg/pg6/pag6.html Steve Heller, USDA, ARS, Plant Genome Project Bldg. 005, Room 337 Beltsville, MD 20705-2350 USA Phone: 301-504-6055 FAX: 301-504-6231 E-mail: srheller@gig.usda.gov WWW: www.hellers.com/~steve From owner-chromosomes@net.bio.net Sat Mar 29 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!EU.net!news.eunet.fi!online.tietokone.fi!flefever From: flefever@ix.netcom.com Newsgroups: bionet.genome.chromosomes Subject: Re: Human Chromosome 7: 7q36 region? Date: Sun, 30 Mar 1997 07:22:27 +0200 Organization: Tietokone Online Lines: 20 Sender: flefever@ix.netcom.com Message-ID: References: <3338F7BE.A50@cephb.fr> <5hkm9j$r6@dfw-ixnews12.ix.netcom.com> NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: flefever@ix.netcom.com(F. Frank LeFever) Subject: Human Chromosome 7: 7q36 region? Newsgroups: bionet.genome.chromosomes Date: 30 Mar 1997 03:22:27 GMT What can you tell me about human chromosome 7, especially stuff at or near 7q35-7q36? AFM199zd4-AFM074xg5? D7S505-D7S483? Or about better places to post this query? Better sources of info than newsgroups? F. Frank LeFever, Ph.D. FAX usa (914) 947-3350 Helen Hayes Hospital flefever@ix.netcom.com West Haverstraw, NY 10993, USA Message-ID: <5hkm9j$r6@dfw-ixnews12.ix.netcom.com> NNTP-Posting-Host: nyc-ny40-24.ix.netcom.com Organization: Netcom References: <3338F7BE.A50@cephb.fr> From owner-chromosomes@net.bio.net Sat Mar 29 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!news.eunet.fi!online.tietokone.fi!srheller From: srheller@GIG.USDA.GOV Newsgroups: bionet.genome.chromosomes Subject: PAG-VI meeting Date: Sat, 29 Mar 1997 22:52:07 +0200 Organization: Tietokone Online Lines: 20 Sender: srheller@GIG.USDA.GOV Message-ID: References: NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: srheller@GIG.USDA.GOV ("Stephen R. Heller") Subject: PAG-VI meeting Newsgroups: bionet.genome.chromosomes Date: 29 Mar 1997 10:52:07 -0800 The first preliminary draft for the PAG-VI meeting is available at: http://probe.nalusda.gov:8000/otherdocs/pg/pg6/pag6.html Steve Heller, USDA, ARS, Plant Genome Project Bldg. 005, Room 337 Beltsville, MD 20705-2350 USA Phone: 301-504-6055 FAX: 301-504-6231 E-mail: srheller@gig.usda.gov WWW: www.hellers.com/~steve Message-ID: NNTP-Posting-Host: net.bio.net Organization: BIOSCI International Newsgroups for Molecular Biology Sender: daemon@net.bio.net From owner-chromosomes@net.bio.net Sat Mar 29 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news1.mpcs.com!news.radio.cz!newsbastard.radio.cz!news.radio.cz!CESspool!news.apfel.de!newsfeed.nacamar.de!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: flefever@ix.netcom.com(F. Frank LeFever) Newsgroups: bionet.genome.chromosomes Subject: Human Chromosome 7: 7q36 region? Date: 30 Mar 1997 03:22:27 GMT Organization: Netcom Lines: 10 Message-ID: <5hkm9j$r6@dfw-ixnews12.ix.netcom.com> References: <3338F7BE.A50@cephb.fr> NNTP-Posting-Host: nyc-ny40-24.ix.netcom.com X-NETCOM-Date: Sat Mar 29 9:22:27 PM CST 1997 What can you tell me about human chromosome 7, especially stuff at or near 7q35-7q36? AFM199zd4-AFM074xg5? D7S505-D7S483? Or about better places to post this query? Better sources of info than newsgroups? F. Frank LeFever, Ph.D. FAX usa (914) 947-3350 Helen Hayes Hospital flefever@ix.netcom.com West Haverstraw, NY 10993, USA From owner-chromosomes@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!geraldo.cc.utexas.edu!not-for-mail From: user@che.utexas.edu (ChE User) Newsgroups: bionet.genome.chromosomes Subject: glowing tobacco plant? Date: 31 Mar 1997 19:35:08 GMT Organization: University of Texas at Austin Lines: 2 Message-ID: <5hp3lc$4d8$10@geraldo.cc.utexas.edu> Reply-To: chingr@weiss.che.utexas.edu NNTP-Posting-Host: silicon.che.utexas.edu X-Newsreader: WinVN 0.92.6+ Anybody have info on the glowing tobacco plant? i'd like to get a hold of one or make one. thanx From owner-chromosomes@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!nntp.ucr.edu!ihnp4.ucsd.edu!gondor!sol.ctr.columbia.edu!spool.mu.edu!uwm.edu!cs.utexas.edu!geraldo.cc.utexas.edu!not-for-mail From: user@che.utexas.edu (ChE User) Newsgroups: bionet.genome.chromosomes Subject: Glowing tobacco plant? Date: 31 Mar 1997 19:34:27 GMT Organization: University of Texas at Austin Lines: 2 Message-ID: <5hp3k3$4d8$9@geraldo.cc.utexas.edu> Reply-To: chingr@weiss.che.utexas.edu NNTP-Posting-Host: silicon.che.utexas.edu X-Newsreader: WinVN 0.92.6+ Anybody have info on the glowing tobacco plant? i'd like to get a hold of one or make one. thanx From owner-chromosomes@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!agate!howland.erols.net!worldnet.att.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!news.eunet.fi!online.tietokone.fi!bruls From: bruls@genethon.fr Newsgroups: bionet.genome.chromosomes Subject: Re: Cannot find gene markers Date: Mon, 31 Mar 1997 16:34:01 +0300 Organization: Tietokone Online Lines: 87 Sender: bruls@genethon.fr Message-ID: References: <5hoeg9$t68@mserv1.dl.ac.uk> NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: Thomas Bruls Subject: Re: Cannot find gene markers Newsgroups: bionet.genome.chromosomes Date: 31 Mar 1997 14:34:01 +0100 Christian Barrett : :In the Nov. 15, 1996 issue of SCIENCE, Polymeropoulos et al. :have mapped the gene for Parkinson's disease to chromosome 4. :In particular, "between markers D4S2361 and D4S421 at a :recombination distance of 0.00 cM from marker D4S1647". : :I have only been able to locate marker D4S421. The other :two don't seem to exist anywhere. I've tried Unigene, :Genethon, and most of the other mapping sites on the web :(like Sanger, Whitehead/MIT, Wellcome Trust -- even though :they're not necessarily involved with Chromosome 4). : :Could somebody explain why all markers would not be available, :some false assumptions I've made in expecting to find them, :or point to a site that I can locate them? : hi, D4S2361 and D4S1647 are CHLC genetic markers: D4S2361=CHLC.ATA2A03.P8247 and his rh id is RH28026 D4S1647=CHLC.GATA2F11.P6975, RH5972, RH28024, STS15576 and was rh mapped on both GB4 and G3 panels: from the Stanford data release: SHGC-17838 000000000000000000010000000000000000000100000110001000100010000100R001000000 0000011 G09195 D4S1647 CHLC.GATA2F11 from EBI: SC Genebridge4 2 0100001101 0000000110 0101010011 0100000100 1002010000 1000001000 0010011000 0001100000 0012100201 211 i hope this helps Thomas ------------------------------------------------------------------------ Thomas Bruls Evry, France bruls@genethon.fr ------------------------------------------------------------------------ Message-ID: <5hoeg9$t68@mserv1.dl.ac.uk> Sender: lpddist@mserv1.dl.ac.uk From owner-chromosomes@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!agate!howland.erols.net!EU.net!news.eunet.fi!online.tietokone.fi!aoijreosiau From: aoijreosiau@a;osieurfasoeur.com Newsgroups: bionet.genome.chromosomes Subject: Jenny Mccarthy Nude Date: Sun, 30 Mar 1997 17:01:44 +0300 Organization: Tietokone Online Lines: 22 Sender: aoijreosiau@a;osieurfasoeur.com Message-ID: References: <5hlro8$hbk@bolivia.earthlink.net> NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: aoijreosiau@a;osieurfasoeur.com Subject: Jenny Mccarthy Nude Newsgroups: bionet.genome.chromosomes Date: 30 Mar 1997 14:01:44 GMT Check out this site, it has tons of Nude Celebrity pics. http://www.sexy-stars.com or if you like young slutty teens go here: http://www.sexy-girls.com Everybody needs some sex in there life. Message-ID: <5hlro8$hbk@bolivia.earthlink.net> NNTP-Posting-Host: 207.217.19.89 Organization: Earthlink Network, Inc. From owner-chromosomes@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Thomas Bruls Newsgroups: bionet.genome.chromosomes Subject: Re: Cannot find gene markers Date: 31 Mar 1997 14:34:01 +0100 Lines: 77 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5hoeg9$t68@mserv1.dl.ac.uk> X-Sun-Charset: US-ASCII Original-To: biochrom@dl.ac.uk Christian Barrett : :In the Nov. 15, 1996 issue of SCIENCE, Polymeropoulos et al. :have mapped the gene for Parkinson's disease to chromosome 4. :In particular, "between markers D4S2361 and D4S421 at a :recombination distance of 0.00 cM from marker D4S1647". : :I have only been able to locate marker D4S421. The other :two don't seem to exist anywhere. I've tried Unigene, :Genethon, and most of the other mapping sites on the web :(like Sanger, Whitehead/MIT, Wellcome Trust -- even though :they're not necessarily involved with Chromosome 4). : :Could somebody explain why all markers would not be available, :some false assumptions I've made in expecting to find them, :or point to a site that I can locate them? : hi, D4S2361 and D4S1647 are CHLC genetic markers: D4S2361=CHLC.ATA2A03.P8247 and his rh id is RH28026 D4S1647=CHLC.GATA2F11.P6975, RH5972, RH28024, STS15576 and was rh mapped on both GB4 and G3 panels: from the Stanford data release: SHGC-17838 000000000000000000010000000000000000000100000110001000100010000100R0010000000000011 G09195 D4S1647 CHLC.GATA2F11 from EBI: SC Genebridge4 2 0100001101 0000000110 0101010011 0100000100 1002010000 1000001000 0010011000 0001100000 0012100201 211 i hope this helps Thomas ------------------------------------------------------------------------ Thomas Bruls Evry, France bruls@genethon.fr ------------------------------------------------------------------------