From owner-chromosomes@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!portc01.blue.aol.com!newsstand.cit.cornell.edu!usenet From: Yangang Bao Newsgroups: bionet.genome.chromosomes Subject: Carrot's chromosomes Date: Thu, 01 May 1997 21:31:01 -0400 Organization: Cornell University Lines: 7 Sender: yb14@cornell.edu (Verified) Message-ID: <336943A3.41C67EA6@sofia.mae.cornell.edu> NNTP-Posting-Host: sofia.mae.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; I; SunOS 4.1.2 sun4c) Hi, Anyone who knows how many chromosomes the carrot(Daucus Carota) has, and how many base pairs it has, please tell me. Especially I want to know the web-site of carrot's genome. Thanx. -bao From owner-chromosomes@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.erols.com!feeder.chicago.cic.net!portc01.blue.aol.com!newsstand.cit.cornell.edu!usenet From: Yangang Bao Newsgroups: bionet.genome.chromosomes Subject: carrot's chromosomes Date: Fri, 02 May 1997 22:18:40 -0400 Organization: Cornell University Lines: 9 Sender: yb14@cornell.edu (Verified) Message-ID: <336AA080.41C67EA6@sofia.mae.cornell.edu> NNTP-Posting-Host: sofia.mae.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (X11; I; SunOS 4.1.2 sun4c) Hi, Is there anyone who knows the web-site that shows the carrot (Daucus Carota)'s chromosomes. I am desperately in need of it!! Greatly thanks. -bao From owner-chromosomes@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!SLU.EDU!SOMARANK From: SOMARANK@SLU.EDU Newsgroups: bionet.genome.chromosomes Subject: Ebola Virus Information Headquarters Date: 2 May 1997 20:32:20 -0700 Organization: SAINT LOUIS UNIVERSITY St. Louis, MO Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IIERTDPACY91Z5OW@SLU.EDU> NNTP-Posting-Host: net.bio.net Hi everybody. My name is Nanda Somarajan. I created a page called Ebola Virus Information Headquarters in mid-march at http://www.angelfire.com/ok/nanda/ I get about 150 - 200 visitors a day to my page. I have links to the Centers for Disease Control's pages on Ebola, the World Headl Organization's pages on Ebola and links to other informative pages about on Ebola Virus. Lately, I have been having problems with getting to my page. I have been having some problems with the Angelfire server. So, I opened up a mirror for my Ebola Virus Information Headquarters page at http://www.geocities.com/CapeCanaveral/Lab/5738/ I have recently put a form so you can send me your comments and suggestions for the page. So, if you are having problems getting to my Ebola Virus Information Headquarters at http://www.angelfire.com/ok/nanda/ Then try my mirro at http://www.geocities.com/CapeCanaveral/Lab/5738/ By the way, please sign my guestbook. From owner-chromosomes@net.bio.net Sat May 03 23:00:00 1997 X-Newsreader: Microsoft Outlook Express 4.71.0544.0 From: "fff" Newsgroups: bionet.genome.chromosomes Subject: trisomy 8 mosiacism Date: Thu, 1 May 1997 11:59:04 -0400 Lines: 5 X-MimeOLE: Produced By Microsoft MimeOLE Engine V4.71.0544.0 NNTP-Posting-Host: 204.151.254.69 Message-ID: <3368bf94.0@eclipse.wincom.net> Path: biosci!rutgers.rutgers.edu!gatech!newsjunkie.ans.net!newsfeeds.ans.net!eclipse.wincom.net! My friend's brother's son has been diagnosed with Trisomy 8 mosiacism.... Is there anyone out there with information about this? Or if anyone have knowledge where to get information they would greatly be indebted From owner-chromosomes@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!Sprint!news-east.sprintlink.net!news-dc-26.sprintlink.net!news-pen-4.sprintlink.net!news.sprintlink.net!news-pen-14.sprintlink.net!stallion.mikuni.com!news From: "Paul Morcos, Ph.D." Newsgroups: bionet.genome.chromosomes Subject: gene structure Date: Tue, 06 May 1997 12:31:49 -0700 Organization: Mikuni Berkeley R&D Corp. Lines: 5 Message-ID: <336F8725.15E3@ucla.edu> NNTP-Posting-Host: kwd1056a.csmc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win16; I) CC: paulm@ucla.edu Dear colleagues, I would like to know if anyone has noticed any association between alternative splicing and change of GT to GC in the consensus sequence of the donor splice site. Please e-mail your replies to S. Sahba at sahbas@csmc.edu Thank you From owner-chromosomes@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!news-peer.gsl.net!portc01.blue.aol.com!audrey02.news.aol.com!not-for-mail From: gwen90@aol.com (GWEN90) Newsgroups: bionet.genome.chromosomes Subject: Urgent: Need a web site to find really good pictures on or about Chromosome 7 or any information about Cystic Fybrosis Date: 6 May 1997 02:54:24 GMT Organization: AOL http://www.aol.com Lines: 11 Message-ID: <19970506025300.WAA00967@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Please Help I am doing a Final Report on Cystic Fybrosis and it counts as half of my total Semester grade. I have almost everything all I really need is PICTURES. If you know of any web sites E-mail them to and I would really appreciate that. Thank You, Somer gwen90@aol.com From owner-chromosomes@net.bio.net Mon May 05 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!newsfeeds.sol.net!uwm.edu!msunews!netnews.upenn.edu!news From: Chris Overton Newsgroups: bionet.genome.chromosomes Subject: Post Doctoral Positions in Computational Genomics/Bioinformatics Date: Tue, 06 May 1997 18:45:40 -0400 Organization: UPenn Lines: 91 Message-ID: <336FB494.3C82@cbil.humgen.upenn.edu> References: <336F8725.15E3@ucla.edu> NNTP-Posting-Host: sibelius.humgen.upenn.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; SunOS 5.4 sun4m) POST-DOCTORAL POSITIONS IN BIOINFORMATICS AND COMPUTATIONAL GENOMICS UNIVERSITY OF PENNSYLVANIA POST-DOCTORAL positions in Bioinformatics and Computational Genomics are available at the University of Pennsylvania through a National Science Foundation Research Training Grant in Computational Biology and as part of the recently established Center for Bioinformatics. Areas of interest include but are not limited to: Development of advanced technology for database evolution, transformation and integration. Data mining and knowledge discovery in biology particularly as applied to distributed systems. Graphical user interfaces and scientific visualization of biological information. Knowledge based systems for high throughput sequence and functional analysis. Computational analysis of signaling pathways and genetic networks especially in eukaryotic systems. Applicants should have a PhD or equivalent with a background in molecular biology, computer and information science, mathematics, statistics or any of the physical sciences. As part of the program in Computational Biology, Computational Genomics, and Bioinformatics, advanced interdisciplinary training is available to exceptional candidates who seek to expand their skill set. The program provides core training in molecular biology and genetics, computer science and engineering, and mathematical statistical modelling so that important biological problems can be addressed effectively through a collaborative effort between researchers in these different fields. Advanced training draws on the expertise of the faculty, and includes both advanced seminars and laboratory research opportunities. Please direct correspondence by mail only to: Dr. G. Christian Overton Director, Center for Bioinformatics University of Pennsylvania Room 1312 Blockley Hall (6021) 418 Guardian Ave Philadelphia, PA 19104-6145 work: (215) 573-3105 fax: (215) 573-3111 url: http://www.cbil.upenn.edu Information required: (a) Completed application form (below). (b) Statement (maximum one page) of your research interests, academic goals, and why you are interested in computational biology. (c) Four letters of reference -- these should be sent directly by the referees. (d) Curriculum vita. Post-doctoral Application Form: Name: Citizenship (US residency status if applicable): Address: Phone: Fax: E-mail contact: Undergraduate GPA and institution: Graduate GPA and institution (if applicable): Degree(s): Coursework (undergraduate and graduate) in Mathematics, Computer Science, Probability and Statistics: Coursework (undergrad. & graduate) in Biology, Molecular Genetics, etc.: Coursework (undergraduate and graduate) in other relevant disciplines: Research interests: Additional relevant comments: From owner-chromosomes@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!warwick!news.nott.ac.uk!ian.graham From: ian.graham@nottingham.ac.uk (Dr Ian Graham) Newsgroups: bionet.genome.chromosomes Subject: Q: K.lactis chromosomes Date: Wed, 07 May 1997 15:44:46 +0100 Organization: University of Nottingham Lines: 12 Message-ID: NNTP-Posting-Host: ppdirg.nottingham.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 A simple question for you all: How many chromosomes does the budding yeast Kluyveromyces lactis have? That's it! Anticipation galore! Ian From owner-chromosomes@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!sol.ctr.columbia.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!usenet From: Victoria Wang Newsgroups: bionet.genome.chromosomes Subject: new data release - mouse genome project Date: Thu, 08 May 1997 18:28:09 -0400 Organization: Whitehead Institute for Biological Research Lines: 242 Message-ID: <33725379.446B@genome.wi.mit.edu> NNTP-Posting-Host: draino.wi.mit.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (X11; I; OSF1 V3.2 alpha) hitehead Institute/MIT Center for Genome Research Mouse Genomic Mapping Project Data Release May 1997 ---------------------------------------------------------------------------- Genetic Map The July 1996 Data Release was the final release of the Mouse Genetic Map at the Whitehead Institute/MIT Center for Genome Research. It reflects the data represented in the 14 March, 1996 issue of Nature. Please see this paper, and also Genetics 131:423-447 (1992) for descriptions of the materials and methods used to construct the maps. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. ---------------------------------------------------------------------------- Mouse Physical Mapping Project Having completed the genetic map, we are now constructing a physical map of the mouse consisting of 10,000 markers screened against a mouse YAC library. Many of the markers are the SSLPs that are also on the genetic map, but are also adding random STSs to the physical map to reach a total of 10,000 markers. In this release, we include data for 7,796 markers successfully screened against the YAC library. We have placed 6,361 of these markers into singly-linked physical map contigs; 5,972 are in doubly-linked contigs. All the markers in doubly-linked contigs are linked to another marker in the contig by at least two YACs, while markers can be joined to a singly-linked contig by only one YAC. Thus, the doubly-linked contigs are generally smaller but more reliable. The table below shows the number of physically and genetically-mapped markers assigned to each chromosome. (Note that some markers that hit yacs have not yet been mapped to a particular chromosome, so the total number of physically-mapped markers in this table represents a subset of the total number of markers in the release.) The right-most columns count the number of markers shared by the two maps ("BOTH"), and the total number of distinct markers mapped in any way ("TOTAL MARKERS"). Breakdown of Mapped Markers by Chromosome CHROMOSOME PHYSICAL GENETIC BOTH TOTAL MARKERS Chr1 565 559 403 789 Chr10 326 317 223 472 Chr11 472 356 280 618 Chr12 329 289 222 423 Chr13 395 317 266 490 Chr14 286 271 202 397 Chr15 327 274 215 435 Chr16 299 215 172 364 Chr17 190 255 107 375 Chr18 271 236 173 367 Chr19 169 134 114 212 Chr2 579 553 388 836 Chr3 363 382 229 571 Chr4 390 367 240 559 Chr5 390 433 268 613 Chr6 395 383 252 583 Chr7 390 356 260 541 Chr8 344 361 262 485 Chr9 371 371 235 559 ChrX 261 230 165 357 TOTAL 7112 6659 4676 10046 YAC Library The YAC library being used is described by Haldi, et al., in Mammalian Genome 7:767-769 (1996). It consists of 40,000 YACs with an average insert size of 820 kb. The entire library, or individual YAC clones, is available through Research Genetics, Huntsville, Alabama (phone, 800-533-4363; outside US/Canada, 205-533-4363; FAX, 205-536-9016) and Genome Systems, Inc. of St. Louis, Missouri, (phone 800-430-0030). We are currently using a subset of 24,000 clones in building the physical map. These 24,000 clones are arrayed here at the Center in a 5-dimensional pooling scheme. The STSs are screened on these 5D pools, with 3 hits describing a single YAC address, and up to two extra hits confirming that address. STSs screened Please note that this release of the physical map represents a work in progress. It includes only those SSLPs that worked well on the first try and that did not require any special attention. There are now 5,112 SSLPs on the physical map, as well as 1671 random STSs and 882 genes and 130 others (RFLPs, monomorphic markers, etc.). In future releases, we expect to add primarily ESTs and random markers. The genetically-mapped SSLPs are screened on the 5-dimensional pools, using the same pair of primers as were used when we placed them on the genetic map. If the marker fails on the first try, it is run through the screening process a second time. If it fails twice, we attempt to convert the SSLP into an STS by picking two new primers and an internal oligo from one side or the other side of the CA-repeat. Markers that fail this final step will probably not be placed on the physical map. If you are working with an SSLP that you want to know the precise placement of in relation to other SSLPs nearby, you may wish to consult the European Collaborative Interpecific Mouse Backcross. They are mapping our SSLPs in a high-resolution 1000-animal backcross. Chromosomes 2, 11, 15, 16, and 17 are completed, chromosomes 3 and 4 are nearly done, and others are underway. Many of the markers on both the genetic and physical map are available through Research Genetics, at a reduced cost under a community discount arrangement set up by the WICGR. (Note: WI/MIT CGR has placed the markers in the public domain. The center and its personnel receive no financial benefit from the sale of primers.) Citing this data Data releases occur on a quarterly basis or more frequently if the amount of new data warrants it. At the end of each quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within two weeks of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy is among the most rapid, broad and regular of any genome center. Its purpose is to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. References to this data in publications should be cited by listing each of the following three sources: 1. Dietrich, W.F., J. Miller, R. Steen, M.A. Merchant, D. Damron-Boles, Z. Husain, R. Dredge, M.J. Daly, K.A. Ingalls, T.J. O'Conner, C.A. Evans, M.M. DeAngelis, D.M. Levinson, L. Kruglyak, N. Goodman, N.G. Copeland, N.A. Jenkins, T.L. Hawkins, L. Stein, D.C. Page, & E.S. Lander (1996) A comprehensive genetic map of the mouse genome. Nature 380:149-152. 2. Dietrich, W.F. et al. (1994) A genetic map of the mouse with 4,006 simple sequence length polymorphisms. Nature Genetics 7:220-245. 3. Copeland, N.G., D.J. Gilbert, N.A. Jenkins, J.H. Nadeau, J.T. Eppig, L.J. Maltais, J.C. Miller, W.F. Dietrich, R.G. Steen, S.E. Lincoln, A. Weaver, D.C. Joyce, M. Merchant, M. Wessel, H. Katz, L.D. Stein, M.P. Reeve, M.J. Daly, R.D. Dredge, A. Marquis, N. Goodman, E.S. Lander (1993) Genome Maps IV. Science 262:67. 4. Supplemented by additional markers in: Whitehead Institute/MIT Center for Genome Research, Genomic Map of the Mouse, Database Release 10, June 1996. Additional publications with related information: Dietrich, W., J. Miller, H. Katz, D. Joyce, R. Steen, S. Lincoln, M. Daly, M.P. Reeve, A. Weaver, P. Anagnostopoulos, N. Goodman, N. Dracopoli, E.S. Lander (1992) Genetic Maps. Stephen J. O'Brien, ed. Cold Spring Harbor Laboratory Press. Assay Conditions for the Genetic Map: Assay conditions and other experimental details are given in: Dietrich, W., et al., 1992. Genetics 131: 423-447. Briefly, the PCR protocol used radiolabeled primers in a 25 cycle PCR (1 '94 degrees, 2' 55 degrees, 3' 72 degrees) on 20 ng of genomic DNA. Contents of this Directory 5-97.MarkerInfo.txt PCR primer and product size 5-97.GeneticMapInfo.txt Genetic mapping information 5-97.PhysicalMapInfo.txt Physical mapping information 5-97.STS2Contig.txt Contig information (singly-linked) 5-97.Contig2STS.txt Contig information - inverted (singly-linked) 5-97.STS2DoubleContig.txt Contig information (doubly-linked) 5-97.DoubleContig2STS.txt Contig information - inverted (doubly-linked) 5-97.Sequences.txt Raw source sequences for STSs pictures/ Maps in postscript and Macintosh format ---------------------------------------------------------------------------- For further information contact: Victoria Wang victoria@genome.wi.mit.edu or Donna Slonim slonim@genome.wi.mit.edu Last modified May 8, 1997 From owner-chromosomes@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!bcm.tmc.edu!hen.imgen.bcm.tmc.edu!ktimms From: ktimms@postmaster@imgen.bcm.tmc.edu (Kirsten M Timms) Newsgroups: bionet.genome.chromosomes Subject: Genexpress cDNAs. Date: 9 May 1997 17:58:13 GMT Organization: Baylor College of Medicine - MHG Lines: 7 Sender: ktimms@hen.imgen.bcm.tmc.edu (Kirsten M Timms) Message-ID: <5kvojl$nj7@gazette.bcm.tmc.edu> NNTP-Posting-Host: hen.imgen.bcm.tmc.edu I'm trying to get hold of a sample of one of the Genexpress cDNAs which has been submitted to Genbank. So far I've been unable to find a source for these clones, or even find out if a source exists. Any info. would be greatly appreciated. Kirsten Timms. From owner-chromosomes@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!darwin.com!jones From: jones@darwin.com (Robert Jones) Newsgroups: bionet.genome.chromosomes Subject: E-mail Address Spoofing Date: 12 May 1997 14:56:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <9705122156.AA27643@olympus.darwin.com> NNTP-Posting-Host: net.bio.net We've received two pieces of unsolictied email sent to the mailing lists biochrom@net.bio.net and staden@net.bio.net regarding a color photographic reprint service called TSP CUSTOM LAB. These emails appear to come from 'franklin@darwin.com' and 'prints@darwin.com'. darwin.com is the domain name of Darwin Molecular Corporation in Bothell, WA. These emails did not originate in Darwin and we have no connection with TSP CUSTOM LAB. The real sender has spoofed the 'from' address. I apologize for this annoyance - and I realize I'm taking up more bandwidth with this note. But I am contacting TSP Custom Lab and 'USA Internet' where the original messages appear to have come from. I would be very interested to hear of other messages that appear to come from darwin.com. The two that I am aware of have the following Message-ID: lines Message-Id: Message-Id: If anyone has any other examples (from darwin.com) I would really like to receive a copy of those, with the full mail headers if possible. I will do my best to prevent this from further inconveniencing anyone. Thanks --Robert Jones jones@darwin.com Director of Computing Darwin Molecular Corporation 1631 220th Street SE Bothell, WA 98021 From owner-chromosomes@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!feed1.news.erols.com!howland.erols.net!vixen.cso.uiuc.edu!ux4.cso.uiuc.edu!jedge From: jennifer jeanette edge Newsgroups: bionet.genome.chromosomes Subject: advice or chit-chat Date: Mon, 12 May 1997 22:48:15 -0500 Organization: University of Illinois at Urbana Lines: 17 Message-ID: NNTP-Posting-Host: ux4.cso.uiuc.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: jedge@ux4.cso.uiuc.edu Hello everyone. I am a junior undergraduate that plans to become a genetic counselor. I have a fellowship with Dr. Helmut Kramer this summer and I was wondering if anyone has heard of his work or has any advice for a future geneticist. Will I have a hard time finding a job after a two year genetic counseling program? Will it be hard to work toward a Ph.D. while working as a counselor? Is a two-year program considered a "cop-out" among the science community? Thanks for any advice. Jennifer jedge@uiuc.edu From owner-chromosomes@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.maxwell.syr.edu!howland.erols.net!newsxfer3.itd.umich.edu!bloom-beacon.mit.edu!senator-bedfellow.mit.edu!lester From: lester@genome.wi.mit.edu (Lester Hui) Newsgroups: bionet.genome.chromosomes Subject: Whitehead/MIT Human Physical Map, Release 11.9 Date: 14 May 1997 23:38:12 GMT Organization: Whitehead Institute for Biological Research Lines: 81 Message-ID: <5ldid4$i4n@senator-bedfellow.MIT.EDU> NNTP-Posting-Host: tango.wi.mit.edu ANNOUNCING: WHITEHEAD INSTITUTE/MIT CENTER FOR GENOME RESEARCH HUMAN GENOMIC MAPPING PROJECT DATA RELEASE 11.9 (MAY 1997) An update to the eleventh release of data from the Human Physical Mapping Project at the Whitehead Institute/MIT Genome Center, covering data generated through the end of April, 1997, is now available. This data release contains radiation hybrid screening data for 14,308 sequence tagged sites (STSs) screened on the Genebridge4 Panel. This represents an additional 1,700 mapped ESTs since the October, 1996 release. Our current integrated map contains 11722 ESTs and 24230 total markers. The data is available electronically in two ways. ANONYMOUS FTP: The entire data release is available as a set of Microsoft Excel files and tab-delimited ascii files on our ftp server. Using an ftp client (such as "Fetch" on the Macintosh), connect to ftp-genome.wi.mit.edu Use "anonymous" as your user name, and give your e-mail address as your password. The data files are present in the directory /distribution/human_STS_releases/oct96. The contents are as follows: 10-96.INTRO.html Introduction to the data release 10-96.STS.DATA.txt STS mapping data as tab-delimited text. 10-96.ALIASES.txt Cross reference of STS names. rhmap/ Radiation hybrid maps. The data is also available in compressed form using the gzip program. THE WORLD-WIDE WEB: You will need a World Wide Web client such as Mosaic (Unix, MS-Windows and Macintosh) or MacWeb (Macintosh). Instruct your client to connect to http://www.genome.wi.mit.edu/ From there, follow the "Human Physical Mapping Project" link. You will be able to browse and download the raw data set, view the individual and integrated maps, and to get information on the radiation hybrid and contig analyses. QUESTIONS AND PROBLEMS. If users have any questions or problems, please contact us at human_STS_help@genome.wi.mit.edu We invite suggestions about how to make these data release most useful. DATA RELEASE POLICY AND CITATION. Data releases are scheduled quarterly. At the end of each quarter, all genomic mapping data are reviewed and prepared for distribution via CGR's electronic databases. Data releases typically occur within a week of the close of the month. Releases are announced by electronic messages posted to the following two newsgroups: "bionet.genome.chromosomes" and "bionet.announce". CGR's data release policy aims to ensure that scientific colleagues have immediate access to information that may assist them in the search for genes. Data releases do not constitute scientific publication of CGR's work, but rather provide scientists with a regular look into our lab notebooks. For projects aimed at the analysis of particular genes or subchromosomal regions, permission is hereby granted to use our data without the need for a formal collaboration, subject only to appropriate acknowledgment. For projects aimed at large-scale mapping of entire chromosomes or entire genomes, use of the data and markers should be on a collaborative basis. The information for the human genome mapping project should be cited as: Whitehead Institute/MIT Center for Genome Research, Human Physical Mapping Project, Data Release 11.9 (May 1997). From owner-chromosomes@net.bio.net Thu May 15 23:00:00 1997 Newsgroups: bionet.genome.chromosomes Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!newshub.csu.net!zdc!super.zippo.com!newsfeed.direct.ca!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!lchan From: lchan@netcom.com (Lui Chan) Subject: Please suggest a good speaker in biotechnology Message-ID: Organization: Netcom On-Line Services X-Newsreader: NN version 6.5.0 CURRENT #9 Date: Fri, 16 May 1997 23:28:12 GMT Lines: 26 Sender: lchan@netcom6.netcom.com Hi, I wonder if anyone know a good speaker in biotechnology? This September, the company I work for will have a science and technology forum in Monterey Bay (Califonia) for the company's engineers (about 130 of us). The forum's goal is to broaden the perspective of our engineers. We would like to have a good speaker in the field of biotechnology. The subject could be (but not limited to) cloning and genetic manipulation/engineering. The audience will be mostly electrical, mechanical and software engineers with strong science background but limited background in biology. So we are looking for a speaker who is not only knowledgeable, but also an effective presenter who can relate his/her work to an audience with limited background. We prefer to find speaker in the Bay area but if the speaker is good, we are more than happy to fly him/her in. So if you know a good speaker, or if you know an organization that can help me to locate a speaker, please drop me a note and I will really appreciate it. Thanks in advance. --Lui (lchan@netcom.com) From owner-chromosomes@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!feed1.news.erols.com!howland.erols.net!portc02.blue.aol.com!audrey02.news.aol.com!not-for-mail From: ssinnge@aol.com (Ssinnge) Newsgroups: bionet.genome.chromosomes Subject: Re: gene structure Date: 16 May 1997 20:20:19 GMT Lines: 7 Message-ID: <19970517002001.UAA06570@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com References: <336F8725.15E3@ucla.edu> Hello, I'm not sure if you can help. One of my day care infants was just diagnosed with neurofibromatosis. Infant has all these coffee stains on his torso. The child is being send to a genetic specialist to confirm the pediatric dermatologist's diagnosis. Is there anything to look for? From owner-chromosomes@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!newshub.csu.net!zdc!super.zippo.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!uwm.edu!msunews!not-for-mail From: Matt Skierkowski Newsgroups: bionet.genome.chromosomes Subject: I need a picture Date: Sat, 17 May 1997 16:51:44 -0400 Organization: National Security Agency ( i wish! ) Lines: 8 Message-ID: <337E1A60.B1F5487C@geocities.com> Reply-To: gavin517@geocities.com NNTP-Posting-Host: pm171-11.dialip.mich.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 X-AUTHid: lepeckik X-Mailer: Mozilla 4.0b4 [en] (Win95; I) X-Priority: 3 (Normal) My name is Matt Skierkowski and I'm doing a project in science about genetics and the races. I need an image that has the usual 22 chromosome and the X and Y, On the Image I also need it to be labeled what the sections are. I need to know the location of things like hair color, skin color, bone marrow type, and any other racial information in genetics. Can anyone Help me? From owner-chromosomes@net.bio.net Sun May 18 23:00:00 1997 Path: biosci!daresbury!uninett.no!stdio!news.radio.cz!europa.clark.net!newsfeed.internetmci.com!in2.uu.net!151.99.250.2!server-b.cs.interbusiness.it!usenet From: "Datalab" Newsgroups: bionet.genome.chromosomes Subject: URGENT HELP!!! Date: 19 May 1997 17:39:18 GMT Organization: DATALAB Lines: 21 Message-ID: <01bc647b$60694d00$436cf3c2@DKT.DATALAB.IT> NNTP-Posting-Host: 194.243.108.67 X-Newsreader: Microsoft Internet News 4.70.1161 We need help for a 6 years old italian girl. She's dying at the Brotzu Hospital of Cagliari - Italy. Doc.Pettinao She has a syndrome characterized from: - Hypercoagulation of the blood - Formation of red thrombus with white core every 15 sec. 2-3 cm. longs Till now it has not been possible to make a diagnosis. If someone has some information that can help to make a diagnosis please get in contact with: lukrezia@mbox.vol.it stefania@alanet.it schintu@pan.bio.uniroma1.it psico@mbox.vol.it Please forward this message to everyone. THANK YOU From owner-chromosomes@net.bio.net Mon May 19 23:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!nntpfeed.doc.ic.ac.uk!sunsite.doc.ic.ac.uk!netcom.net.uk!ix.netcom.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!howland.erols.net!surfnet.nl!news-zh.switch.ch!news-ge.switch.ch!newscore.univie.ac.at!02-newsfeed.univie.ac.at!garion.telecom.at!news-admin@telecom.at From: Omnicron Newsgroups: bionet.genome.chromosomes Subject: Higher-ranked genetic information Date: Tue, 20 May 1997 18:23:36 +0100 Organization: CPIS Lines: 20 Message-ID: <3381D008.D4E74CCA@hotmail.com> NNTP-Posting-Host: 194.118.46.216 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.0b4 [en] (Win95; I) X-Priority: 3 (Normal) I'm sorry in case this is not the right newsgroup for my question, but it seems to be quite close. Some days ago I heared from TV that scientists detected a certain form of hereditary information that is not really based on nucleotides, but any kind of a FIELD or something like that. Un- fortunately I did not understand the word for this "novelty" and as this was no boulevard-TV program I should believe that there was something with it. Does anyone know sth about this? Is there any known medium on a genetic or similar basis which is not involved with genes as is? Thanks in advance for your reply .Ocr. From owner-chromosomes@net.bio.net Tue May 20 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!newshub.sdsu.edu!newshub.csu.net!www.nntp.primenet.com!nntp.primenet.com!news.maxwell.syr.edu!newsfeed.internetmci.com!in1.uu.net!152.163.170.17!newstf01.news.aol.com!audrey02.news.aol.com!not-for-mail From: robertoalf@aol.com (Robertoalf) Newsgroups: bionet.genome.chromosomes Subject: Re: Please suggest a good speaker in biotechnology Date: 20 May 1997 20:09:50 GMT Lines: 9 Message-ID: <19970521000900.UAA24995@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com References: I strongly recommend Dr. Norman Pace from Berkeley. He is a great speaker who specialized in the characterization of non-cultivable microorganisms by DNA sequencing. His P&G Award Lecture at ASM last year was published in the ASM news in February or March I think. It was an enjoyable lecture. Good Luck, Roberto Rodriguez, Ph.D. Pharmacia Biotech From owner-chromosomes@net.bio.net Wed May 21 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: kristoff (David Kristofferson) Newsgroups: bionet.genome.chromosomes Subject: IMPORTANT - BIOSCI moving to Stanford! Date: 21 May 1997 21:08:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705220406.VAA04657@net.bio.net> NNTP-Posting-Host: net.bio.net After more than a decade of serving the biology community on the BIOSCI project, I have decided that it is time for me to pass the torch. The demands of my career and family are making it increasingly difficult for me to run BIOSCI adequately on a part-time basis, and I've decided that I would do the project more harm than good by clinging to it. Therefore I have concluded an agreement with the Stanford University Libraries to take over the management of the BIOSCI project effective 6 June 1997. Many of you know the work of the Stanford Libraries through HighWire Press which has done an excellent job bringing the Journal of Biological Chemistry, Science Magazine, and other prestigious journals to the WWW (see http://highwire.stanford.edu/). I have every confidence that Stanford will provide an environment where the BIOSCI project can flourish. The BIOSCI team at Daresbury will continue to support the project in Europe, and Dave Mack and Julie Lawrence on the current BIOSCI staff will continue to assist the new team at Stanford. The project will be managed by Serge Taylor of the Stanford Libraries who is responsible for developing Web-based "knowledge environments" for scientists. I will still participate with the project in an advisory capacity but have to phase myself out of day-to-day operations. Many of you who read bionet.announce, bionet.biology.computational, and bionet.jobs.offered (the groups that I moderate) have been aware of the recent posting delays that my busy schedule has inflicted on those newsgroups. I am pleased to announce that Todd McGee, Scientific Advisor of the HighWire Press, will be taking over moderation of those groups from me this week. ** NOTE ** - The BIOSCI hardware will be down for backups on 6 June and will be moved over the weekend of June 7 and 8. We will be changing the IP number of the machine, but the host name and all mailing addresses will remain unchanged. There may be a few days of service disruption during this time due to the move unfortunately, just as we experienced in BIOSCI's previous move. We hope to keep this to a minimum. We will post status reports on the move to bionet.announce before and after it happens. Please watch that newsgroup for details. It has been a pleasure serving all of you since the "early days" of biology on the Internet. I've had the pleasure of not only "knowing" many of you over the Internet, but have also met literally thousands of biologists around the U.S. and elsewhere during the many Internet training seminars that I gave over the last decade. I've been truly fortunate to have been given the chance to play a role in the growth of biology on the Internet. This is something I will always treasure. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!daresbury!uninett.no!due.unit.no!nntp.uio.no!news.apfel.de!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!in2.uu.net!152.163.170.17!newstf01.news.aol.com!audrey01.news.aol.com!not-for-mail From: naturemag@aol.com (Naturemag) Newsgroups: bionet.genome.chromosomes Subject: Yeast Genome Sequence soon to be published Date: 23 May 1997 10:33:56 GMT Lines: 6 Message-ID: <19970523103301.GAA12735@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Organization: AOL, http://www.aol.co.uk The complete sequence of the Saccharomyces Cerevisiae genome will be published as a supplement to Nature with the issue of 29 May. A News and Views on the subject together with details of how to obtain the printed supplement (and CD-ROM) will appear on the Nature website on that day (12 midnight GMT). From owner-chromosomes@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!ais.net!newsfeed.direct.ca!news.uoregon.edu!news.acsu.buffalo.edu!acsu.buffalo.edu!usenet From: xz4@acsu.buffalo.edu Newsgroups: bionet.genome.chromosomes Subject: mouse-fly synteny Date: Fri, 23 May 1997 18:06:43 -0400 Organization: University at Buffalo School of Medicine and Biomedical Sciences Lines: 9 Message-ID: <338614F3.61FF@acsu.buffalo.edu> Reply-To: xz4@acsu.buffalo.edu NNTP-Posting-Host: mettinger2.med.buffalo.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-User: xz4 X-Mailer: Mozilla 3.01Gold (Win95; I) Hi, Does anyone here know if there any studies or information on the gene synteny between mouse and Drosophila chromosomes? Any information would be highly appreciated. Xianbo xz4@acsu.buffalo.edu From owner-chromosomes@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.nacamar.de!fu-berlin.de!rag3.rz-berlin.mpg.DE!not-for-mail From: andy@rag3.RZ-Berlin.MPG.DE (Andrei Grigoriev) Newsgroups: bionet.genome.chromosomes Subject: genome navigator Date: 27 May 1997 14:27:33 GMT Lines: 20 Message-ID: <5mer0l$nss@fu-berlin.de> NNTP-Posting-Host: rag3.rz-berlin.mpg.de (141.14.130.162) X-Access: 16 25 816 X-Newsreader: TIN [version 1.2 PL2] Genome Navigator is an attempt to provide a visual interactive gateway to major databases containing physical and genetic mapping information about human, mouse and yeast genomes. Genomic maps of these organisms are displayed using DerBrowser, a Java applet, designed as a universal tool to display and navigate various types of maps. Among other features, it allows a user to query external databases about any map object. The list of data sources currently includes: Whitehead/MIT, GDB, CEPH-Genethon Infoclone, CHLC, Human Transcript Map, and several human chromosome-specific databases; EUCIB, Saccharomyces Genome Database, YPD, MIPS and GeneQuiz server. http://www.mpimg-berlin-dahlem.mpg.de/~andy/GN/ Andrei Grigoriev Max-Planck-Institute for Molecular Genetics, Ihnestr. 73, 14195 Berlin-Dahlem, Germany From owner-chromosomes@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 May 1997 02:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705270900.CAA12732@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-chromosomes@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!europa.clark.net!newsfeed.internetmci.com!jump.net!grunt.dejanews.com!not-for-mail Date: Tue, 27 May 1997 00:41:11 -0600 From: telenius@istar.ca Subject: how do I concentrate metaphase chromosomes? Newsgroups: bionet.genome.chromosomes,bionet.molbio.methds-reagnts Message-ID: <864711592.28282@dejanews.com> Reply-To: telenius@istar.ca Organization: Deja News Usenet Posting Service X-Article-Creation-Date: Tue May 27 05:39:53 1997 GMT X-Originating-IP-Addr: 204.191.155.88 (ts10-09.vcr.iSTAR.ca) X-Http-User-Agent: Mozilla/2.01 (Macintosh; I; 68K) X-Authenticated-Sender: telenius@istar.ca Lines: 17 Xref: biosci bionet.genome.chromosomes:1650 bionet.molbio.methds-reagnts:57980 I need to take 300,000 metaphase chromosomes and put them in a volume of 20 microliter. Does anyone have protocol/suggestion for how to concentrate metaphase chromosomes in a gentle manner so as not to damage their integrity? The need to remain 'viable' after the concentration step. Thank you! -------------------==== Posted via Deja News ====----------------------- http://www.dejanews.com/ Search, Read, Post to Usenet From owner-chromosomes@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!news-peer.gsl.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!worldnet.att.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: What Percentage of Genes are Alternately Spliced (or how many)? Date: 28 May 1997 23:21:48 GMT Organization: University of Washington, Seattle Lines: 26 Message-ID: <5miemc$584@nntp3.u.washington.edu> NNTP-Posting-Host: saul1.u.washington.edu NNTP-Posting-User: roach Does anybody have any data or even a good guess on this subject? How many characterized genes are known to be alternately spliced? This out of how many characterized genes? I guess I am most interested in human, but will be fascinated by data from any organism. Any bets on what percentage of human genes will be shown to be alternately spliced once we get done with this whole genome thing? And what will be the average/range/distribution of the number of differently spliced products from a single gene? Thanks in advance for any replies. -Jared ------------------------------------------------------------------ Jared C. Roach Department of Molecular Biotechnology Health Sciences Building, Room K354 University of Washington Box 357730 Seattle, WA 98195 phone (206) 616-4536 FAX (206) 685-7301 roach@u.washington.edu http://weber.u.washington.edu/~roach/ From owner-chromosomes@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!STUDENTS.WISC.EDU!jtmille1 From: jtmille1@STUDENTS.WISC.EDU (Joe Miller) Newsgroups: bionet.genome.chromosomes Subject: mapping large segments Date: 29 May 1997 12:49:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am working on a 100kb BAC fragment and have subcloned several repetitive and single copy sequences (all less than 3 kb). I would like to make a restriction map of the entire BAC. Other than subcloning smaller fragments (e.g. 10-50 kb) which could take a long time to dissect and order does anyone have any suggestions for making a broad baseline map of the entire BAC. Thanks Joe Miller Department of Horticulture University of Wisconsin-Madison From owner-chromosomes@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!newsfeed.direct.ca!portc01.blue.aol.com!audrey02.news.aol.com!not-for-mail From: bezuhofii@aol.com (BEZUHOFII) Newsgroups: bionet.genome.chromosomes Subject: turner syndrome Date: 30 May 1997 02:19:20 GMT Lines: 2 Message-ID: <19970530021900.WAA28999@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I would like to receive any information anyone can provide me about this condition From owner-chromosomes@net.bio.net Thu May 29 23:00:00 1997 From: alm13@cornell.edu (Andreas Matern) Newsgroups: bionet.genome.chromosomes Subject: Re: turner syndrome Date: Fri, 30 May 1997 10:47:14 -0400 Organization: Cornell University Lines: 19 Sender: alm13@cornell.edu (Verified) Message-ID: References: <19970530021900.WAA28999@ladder02.news.aol.com> NNTP-Posting-Host: 128 Path: biosci!rutgers.rutgers.edu!gatech!news1.mid-ga.com!nntp.mid-ga.com!news.oru.edu!news.IAEhv.nl!usenet.logical.net!news.radio.cz!europa.clark.net!worldnet.att.net!howland.erols.net!news3.cac.psu.edu!news.tc.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user In article <19970530021900.WAA28999@ladder02.news.aol.com>, bezuhofii@aol.com (BEZUHOFII) wrote: > I would like to receive any information anyone can provide me about this > condition Try: http://www.turner-syndrome-us.org/ Sincerely, -- Andreas Matern alm13@cornell.edu 266 Emerson Hall Dept of Plant Breeding and Biometry Cornell University Ithaca, NY 14853 From owner-chromosomes@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!gondor!sol.ctr.columbia.edu!news.msfc.nasa.gov!europa.clark.net!worldnet.att.net!news.u.washington.edu!roach From: roach@u.washington.edu (Jared Roach) Newsgroups: bionet.genome.chromosomes Subject: Re: What Percentage of Genes are Alternately Spliced (or how many)? Date: 30 May 1997 23:08:31 GMT Organization: University of Washington, Seattle Lines: 28 Message-ID: <5mnmlf$so6@nntp3.u.washington.edu> References: <5miemc$584@nntp3.u.washington.edu> NNTP-Posting-Host: saul7.u.washington.edu NNTP-Posting-User: roach a quick QUERY of GenBank release 99 under ACNUC (http://acnuc.univ-lyon1.fr/) gives the following results : number of human sequences with a coding sequence (sp=homo sapiens AND k=cds) : 22794 number of these with a key word of alternative splicing (k=@alt@splic@) : 268 -> 268 / 22794 = 1.2% for all vertebrates : 593 / 60922 = 1.0% Of course this is very crude, but I hope it helps a little. Marc _________________________________________________________________ Marc Robinson UMR CNRS 5558 "Biometrie, Genetique, et Biologie des Populations" Universite Claude Bernard Lyon 1 43, boulevard du 11 novembre 1918 69622 Villeurbanne cedex France tel : +33 4 72 44 80 00 - poste 42 59 fax : +33 4 78 89 27 19 e-mail : robinson@biomserv.univ-lyon1.fr La liberte ne s'use que quand on ne s'en sert pas. _________________________________________________________________