From owner-chromosomes@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!ai-lab!eecs-usenet-02.mit.edu!xfer02.netnews.com!howland.erols.net!portc02.blue.aol.com!audrey02.news.aol.com!not-for-mail From: shinrichs9@aol.com (SHinrichs9) Newsgroups: bionet.genome.chromosomes Subject: Cytochrome C & Lambda Repressor DNA sequences Date: 2 Jul 1997 13:16:10 GMT Lines: 55 Message-ID: <19970702131600.JAA04943@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I am interested in comparing certain DNA sequences in order to investigate ancestral relationships. Thus, I am requesting DNA sequences from informed people who might be able to provide references. If one can I would appreciate them sending them to me at Shinrichs9@aol.com. Thanks. I am most interested in the human and chimpanzee DNA sequences for the Cytochrome C protein. Also, I am interested in the human and chimpanzee DNA sequences for the lambda repressor. In addition, as a second priority I am interested in the DNA sequences for the following species for the above mentioned molecules. Type Species Mammals Man Mammals Rhesus monkey Mammals Horse Mammals Donkey Mammals Cow, Pig, Sheep Mammals Dog Mammals Rabbit Mammals Cal. Grey Whale Mammals Great gray kangaroo Vert. Chicken, turkey Vert. Pigeon Vert. Pekin duck Vert. Snapping turtle Vert. Rattlesnack Vert. Bullfrog Vert. Tuna Vert. Dogfish Insects Samia cynthia (a moth) Insects Tobacco hornworm moth Insects Screworm fly Insects Drosphila (fruit fly) Insects Baker's yeast Insects Candida krusei (a yeast) Insects Neurospara crassa (a mold) Higher plants Wheat germ Higher plants Buckwheat seed Higher plants Sunflower seed Higher plants Mung bean Higher plants Cauliflower Higher plants Pumpkin Higher plants Sesame seed Higher plants Castor bean Higher plants Cottonseed Higher plants Abutilon seed SHinrichs9@aol.com http://members.aol.com/SHinrichs9/ Please email me if you post so I am aware, thank-you From owner-chromosomes@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!newsfeed.dacom.co.kr!newsfeed.direct.ca!News1.Vancouver.iSTAR.net!news.istar.net!EU.net!blackbush.xlink.net!news-ge.switch.ch!in2p3.fr!univ-lyon1.fr!news From: robinson@evomol.univ-lyon1.fr (Marc Robinson) Newsgroups: bionet.genome.chromosomes Subject: Re: Cytochrome C & Lambda Repressor DNA sequences Date: 2 Jul 1997 17:40:02 GMT Organization: Universite Claude Bernard - Lyon 1 Lines: 28 Message-ID: <5pe3pi$hbd@tempo.univ-lyon1.fr> References: <19970702131600.JAA04943@ladder02.news.aol.com> Reply-To: robinson@evomol.univ-lyon1.fr NNTP-Posting-Host: evomol.univ-lyon1.fr In article <19970702131600.JAA04943@ladder02.news.aol.com>, shinrichs9@aol.com (SHinrichs9) writes: >I am interested in comparing certain DNA sequences in order to investigate >ancestral relationships. Thus, I am requesting DNA sequences from informed >people who might be able to provide references. If one can I would >appreciate them sending them to me at Shinrichs9@aol.com. Thanks. http://acnuc.univ-lyon1.fr/ at this site you can query a number of sequence databases through gene name, taxonomical units including species name, keywords, etc. hope this helps, Marc _________________________________________________________________ Marc Robinson UMR CNRS 5558 "Biometrie, Genetique, et Biologie des Populations" Universite Claude Bernard Lyon 1 43, boulevard du 11 novembre 1918 69622 Villeurbanne cedex France tel : +33 4 72 44 80 00 - poste 42 59 fax : +33 4 78 89 27 19 e-mail : robinson@biomserv.univ-lyon1.fr La liberte ne s'use que quand on ne s'en sert pas. _________________________________________________________________ From owner-chromosomes@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!rutgers!news.sgi.com!news-peer.gsl.net!news.gsl.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: huntpharm@aol.com (Huntpharm) Newsgroups: bionet.genome.chromosomes Subject: job opportunity in bioinformatics Date: 2 Jul 1997 22:44:49 GMT Lines: 11 Message-ID: <19970702224400.SAA10462@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I am looking for a person to head up bioinformatics. As a visionary you will be responsible for leading a group of five and taking the bioinformatics team to the next level. You will lead this group responsible for the application of computer technology to genomic analysis and research. We are a leading pharmaceutical company with research facilities in Connecticut and can provide excellent benefits (health insurance, dental and vision plans, paid vacations and more. Excellent compensation package. A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 EXT. 218, fax CV to 609-584-9575 or E-Mail to SIS@diedremoire.com or Neurohunt@aol.com. From owner-chromosomes@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!CCVAX.SINICA.EDU.TW!bocmchen From: bocmchen@CCVAX.SINICA.EDU.TW (cmchen) Newsgroups: bionet.genome.chromosomes Subject: post-doctoral position available Date: 2 Jul 1997 23:33:17 -0700 Organization: IBAS Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BBB777.3654@ccvax.sinica.edu.tw> Reply-To: bocmchen@ccvax.sinica.edu.tw NNTP-Posting-Host: net.bio.net Post-doctoral Research Fellow A post-doctoral research position is available immediately. The work involves in situ hybridization and molecular analysis of Phalaenopsis species and hybrids. Candidates should have a Ph.D. degree in Genetics or a related discipline. The project is supported by National Science Council of the Republic of China. Initial appointment is for one year, with the possibility of extension for two more years. Send a letter of interest, curriculum vitae and two letters of recommendation to: Prof. Chi-Chang Chen, Department of Botany, National Taiwan University, Taipei, Taiwan. Tel: 886-2-3630231 ext 2371; Fax: 886-2-3918940. or write to the E-mail address: bocmchen@ccvax.sinica.edu.tw. From owner-chromosomes@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!www.nntp.primenet.com!nntp.primenet.com!news.maxwell.syr.edu!ais.net!su-news-hub1.bbnplanet.com!news.bbnplanet.com!venus.sun.com!wnoc-sfc-news!wnoc-tyo-news!news.nc.u-tokyo.ac.jp!news From: "Shou Serizawa" Newsgroups: bionet.genome.chromosomes Subject: YAC library Date: 3 Jul 1997 03:18:48 GMT Organization: Network Operation Centre, The University of Tokyo Lines: 9 Message-ID: <01bc8760$a0f12660$c4200b85@kawauso.biochem.s.u-tokyo.ac.jp> NNTP-Posting-Host: neuron.biochem.s.u-tokyo.ac.jp X-Newsreader: Microsoft Internet News 4.70.1161 Does anybody know of YAC library of mice, having a large insert in the clone, like 1-2Mbp. I already know of YAC library having an insert of 500Kb on average. Thanks in advance. Shou Serizawa Dept. of Biophysics and Biochemistry The University of Tokyo From owner-chromosomes@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!OBZOR.BIO21.ACAD.BG!ttsonev From: ttsonev@OBZOR.BIO21.ACAD.BG (biofac) Newsgroups: bionet.genome.chromosomes Subject: Help, please! Date: 3 Jul 1997 10:23:03 -0700 Organization: Institute of Plant Physiology Lines: 137 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BBDFA0.D8F4F4BB@obzor.bio21.acad.bg> NNTP-Posting-Host: net.bio.net Sofia University "St Kliment Ohridsky"
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  From owner-chromosomes@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!NCGR.ORG!gas From: gas@NCGR.ORG ("Gustavo A. Seluja") Newsgroups: bionet.genome.chromosomes Subject: classical genetics question Date: 3 Jul 1997 10:06:08 -0700 Organization: NCGR Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BBDBC4.1133@ncgr.org> Reply-To: gas@ncgr.org NNTP-Posting-Host: net.bio.net Perhaps I can get a definite answer to the following question. Who is more genetically similar: 2 brothers or father and son? Here, I'm thinking of nuclear material, and not mitochondrial genome. Thanks to anyone interested in answering this question! (references will be most welcome) -- Gustavo A. Seluja, MSc Bioinformatics Associate National Center for Genome Resources 1800 Old Pecos Trail, Suite A Santa Fe, NM 87505 USA mailto:gas@ncgr.org http://www.ncgr.org/ ph: 505-982-7840 - operator ph: 505-995-4453 - direct fax: 505-982-7690 From owner-chromosomes@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!server1.netnews.ja.net!server5.netnews.ja.net!server3.netnews.ja.net!baron.netcom.net.uk!netcom.net.uk!dispatch.news.demon.net!demon!europa.clark.net!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!venus.sun.com!wnoc-sfc-news!wnoc-tyo-news!news.nc.u-tokyo.ac.jp!news From: "Shou Serizawa" Newsgroups: bionet.genome.chromosomes Subject: YAC library Date: 3 Jul 1997 03:22:09 GMT Organization: Network Operation Centre, The University of Tokyo Message-ID: <01bc8761$1917d120$c4200b85@kawauso.biochem.s.u-tokyo.ac.jp> NNTP-Posting-Host: neuron.biochem.s.u-tokyo.ac.jp X-Newsreader: Microsoft Internet News 4.70.1161 Lines: 9 Does anybody know of YAC library of mice, having a large insert in the clone, like 1-2Mbp. I already know of YAC library having an insert of 500Kb on average. Thanks in advance. Shou Serizawa Dept. of Biophysics and Biochemistry The University of Tokyo e-mail: ss77190@hongo.ecc.u-tokyo.ac.jp From owner-chromosomes@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!rutgers!dziuxsolim.rutgers.edu!uunet!in3.uu.net!194.162.162.196!newsfeed.nacamar.de!howland.erols.net!news-peer.sprintlink.net!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!207.20.0.4!newsfeed2.aimnet.com!news.internetMCI.com!not-for-mail From: drtpttpotgrt@elprpypspfo.com Newsgroups: bionet.genome.chromosomes Subject: !!!Your ONENUMBER for everything!!!! Date: 6 Jul 1997 03:30:07 GMT Organization: Internet MCI Lines: 31 Message-ID: <5pn3fv$6n7$1@news.internetmci.com> NNTP-Posting-Host: usr2-dialup3.mix1.bloomington.mci.net One-Number service puts you in control of your calls while ensuring that your calls, messages and faxes reach you regardless of your location. 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Instead, we offer service to integrate your existing products, adding value to what you already have by letting your phones, pagers, fax machines and voice-mail systems work together seamlessly. To learn more please visit our web site at: http://www.mynumber.com Please do not reply by e-mail as we are not set up to respond to e-mail requests. From owner-chromosomes@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!TEOSINTE.AGRON.MISSOURI.EDU!ed From: ed@TEOSINTE.AGRON.MISSOURI.EDU (Ed Coe) Newsgroups: bionet.genome.chromosomes Subject: Postdoctoral: Mutants in the Maize Genome Date: 6 Jul 1997 10:38:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BFDA7F.A31@teosinte.agron.missouri.edu> NNTP-Posting-Host: net.bio.net Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Maize is a species in which the incredible resource of mutants is barely tapped. This postdoctoral position is to oversee, conduct, and advance novel research approaches for mapping of visible-expression genes; to position these genes with cDNAs; and to seek evidence of biochemical functions. This is an opportunity to experience the wide range of mutants in maize; high-efficiency mapping; and strategies by which to follow molecular clues to gene functions. Other ongoing research in the group includes studies in quantitative trait inheritance; gene expression and relationship to traits; and the Maize Genome database and informatics, which are opportunities for additional experience. Appointee will be a participant in the University of Missouri maize group, which has broad training and research strengths that range across breeding, molecular pathology, entomology, physiological genetics, organelle genetics, cytogenetics, cell biology, and gene regulation. Conduct of the project requires expertise in molecular genetics, and knowledge pertinent to plant physiology and biochemistry. Review of applications will begin immediately and will continue until the position is filled. Send a CV; brief statement of research interests; and names, phone numbers, and email addresses of 3 references to: Dr. Edward Coe, 210 Curtis Hall, University of Missouri, Columbia, MO 65211, ed@teosinte.agron.missouri.edu, phone 573-882-2768, fax 573-884-7850. Women and minorities are encouraged to apply. The University of Missouri is an EEO/AA/ADA employer. From owner-chromosomes@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!LIGHT.IINET.NET.AU!bethj From: bethj@LIGHT.IINET.NET.AU (Beth Templeton) Newsgroups: bionet.genome.chromosomes Subject: cloning fossil pollen Date: 6 Jul 1997 03:43:46 -0700 Organization: iiNet Technologies (SA) Pty Ltd Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <33BF7622.F6DD4B78@light.iinet.net.au> Reply-To: bethj@light.iinet.net.au NNTP-Posting-Host: net.bio.net hi , just a thought knowing that pollin chromosomes are most likely intact ,they may be able to be isolated and cloned using a anucleated plant cell which is then generated in a medium. Pollin chromosomes are diploid I believe this may be a problem. Comment? From owner-chromosomes@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!daresbury!lyra.csx.cam.ac.uk!server1.netnews.ja.net!server5.netnews.ja.net!server3.netnews.ja.net!baron.netcom.net.uk!netcom.net.uk!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!portc02.blue.aol.com!audrey02.news.aol.com!not-for-mail From: rcjohnsen@aol.com (Rcjohnsen) Newsgroups: bionet.genome.chromosomes Subject: Re: classical genetics question Date: 6 Jul 1997 09:00:55 GMT Lines: 6 Message-ID: <19970706090001.FAA20961@ladder02.news.aol.com> NNTP-Posting-Host: ladder02.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com References: <33BBDBC4.1133@ncgr.org> I should think a father and son are more genetically related than two brothers. The son shares half his genetic material with his father. The same is true for the brother but brother two may share parts of the fathers genome that brother one doesn't have. And don't for the process of crossing over. That tends to make everyone genetically unique Rcjohnsen@aol.com From owner-chromosomes@net.bio.net Sun Jul 06 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.direct.ca!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!128.228.4.60!news.cuny.edu!doc.mssm.edu!fud01 From: DFU Newsgroups: bionet.genome.chromosomes Subject: Re: classical genetics question Date: Mon, 7 Jul 1997 17:43:13 -0400 Lines: 32 Message-ID: References: <33BBDBC4.1133@ncgr.org> NNTP-Posting-Host: doc.mssm.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To: "Gustavo A. Seluja" In-Reply-To: <33BBDBC4.1133@ncgr.org> On 3 Jul 1997, Gustavo A. Seluja wrote: > Perhaps I can get a definite answer to the following question. Who is > more genetically similar: 2 brothers or father and son? Here, I'm > thinking of nuclear material, and not mitochondrial genome. Thanks to > anyone interested in answering this question! (references will be most > welcome) > > -- > Gustavo A. Seluja, MSc > Bioinformatics Associate > National Center for Genome Resources > 1800 Old Pecos Trail, Suite A > Santa Fe, NM 87505 > USA > > mailto:gas@ncgr.org > http://www.ncgr.org/ > ph: 505-982-7840 - operator > ph: 505-995-4453 - direct > fax: 505-982-7690 > > Theoreticaly, both situations have the same amount of genetic material of the same origin. However, brothers have more complicated (by 2X difference) haplotype than the father and son do. Putting them together, the father and son are more genetically similar. From owner-chromosomes@net.bio.net Mon Jul 07 23:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!fu-berlin.de!news-ber1.dfn.de!news-ham1.dfn.de!news-han1.dfn.de!tubsibr!rzlimes.gbf-braunschweig.de!not-for-mail From: "Dr. Thomas Heinemeyer" Newsgroups: bionet.genome.chromosomes Subject: TRANSFAC 3.2 database Date: Tue, 08 Jul 1997 08:38:37 +0200 Organization: Ges. f. Biotechn. Forsch. mbH (non profit) Lines: 86 Message-ID: <33C1E06D.121F@gbf-braunschweig.de> NNTP-Posting-Host: gen219.gbf-braunschweig.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (WinNT; I) TRANSFAC 3.2 is available now at: http://transfac.gbf.de. On the TRANSFAC server, you will find also the sequence analysis programs PatSearch MatInspector SaGa FastM and Thure Etzold's SRS5 with a large collection of databases. TRANSFAC is a database about eukaryotic transcription factors and their binding sites. It consists of six cross-linked tables: SITE CELL FACTOR CLASS MATRIX GENE It is also cross-linked with TRRD (Transcription Regulatory Region Database) and COMPEL from the ICG, Novosibirsk (N. A. Kolchanov, A. E. Kel). It contains numerous cross-references to external databases such EMBL, SWISSPROT, PIR, FLYBASE, EPD, and PROSITE. For further details see Wingender et al., Nucleic Acids Res. 25:265-268, 1997. NEW FEATURES are: - Additional FACTOR and SITE entries, - cross-references to PDB, - comprehensive linkage of FACTOR entries with a proposed transcription factor classification sytem (http://transfac.gbf.de/TRANSFAC/cl/cl.html). The TRANSFAC database comes along with several sequence analysis tools such as - PatSearch, which uses the sequence information contained in the SITE table for analysis of submitted sequences, - MatInspector, using a library of matrices selected from the TRANSFAC MATRIX table (see Quandt et al., Nucleic Acids Res. 23:4878-4884, 1995). Moreover, the TRANSFAC server provides a new program (SaGa: structural analysis with genetic algorithms, developed by Stefan Meier) which can be used to identify structural characteristics in the environment of aligned functional sites, e.g., transcription factor binding sites. SaGa uses a library of structural parameters developed by H. Sklenar and coworkers (MDC, Berlin; see Karas et al., CABIOS 12:441-446, 1996). The SRS5 system implemented on the TRANSFAC server comprises the following databases, in addition to the TRANSFAC tables: EMBL, EMBLNEW SWISSPROT, SWISSNEW TREMBL REMTREMBL SPTREMBL PIR EPD PDB PROSITE ENZYME EMBLNEW is now updated daily on the TRANSFAC SRS-Site with the new files from European Bioinformatics Institute (EBI) in Hinxton. Edgar Wingender Thomas Heinemeyer -- Dr. Thomas Heinemeyer Tel.: ++49(0)531 6181 295 Ges. f. Biotechn. Forsch. mbH Fax: ++49(0)531 6181 266 Abt. Genomanalyse E-Mail: thh@gbf.de Mascheroder Weg 1 http://transfac.gbf.de/Staff/thh.html D-38124 Braunschweig From owner-chromosomes@net.bio.net Tue Jul 08 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.genome.chromosomes Organization: Yankee Inc. Subject: !!!Hello!!! NNTP-Posting-Host: 205.139.183.49 Message-ID: <33c0b439.0@nntp.kalnet.net> Date: 7 Jul 97 09:17:45 GMT Lines: 9 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.49 Are you looking for a great place to find interesting things on the WWW??? Than look no more because you have found the right place!!! The place you are looking for is Yankee Inc.!!! A great Web Site full of interesting links to interesting places!!! Everything from Adult to Xzlyaphone it's here!!! There is something for everyone and then some!!! So give it a try what do you have to lose except some sleep??? Yankee Inc. http://www.kalnet.net/yank714 Yankee Inc. Your Alternative Web Solution!!! Yankee Inc. http://www.kalnet.net/yank714 If you would like to be removed from my mailing list - hit reply and type "REMOVE" and I will promptly remove you from my list!!! Thank You!!! From owner-chromosomes@net.bio.net Tue Jul 08 23:00:00 1997 Path: biosci!rutgers!gatech!newsxfer3.itd.umich.edu!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: huntpharm@aol.com (Huntpharm) Newsgroups: bionet.genome.chromosomes Subject: job opportunity for protein chemist Date: 9 Jul 1997 11:35:07 GMT Lines: 13 Message-ID: <19970709113500.HAA27867@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Organization: AOL http://www.aol.com I am looking for a Protein Chemistry Scientist to develop cancer therapeutic proteins and small molecules. This work would also include modeling drugs which interact with small molecules. You need to have a PhD, be a protein biochemist with 1-6 years post doc experience in protein engineering and a strong background in creative molecular modeling is required. Recombinant molecular biology skills would be a plus. This person will have a strong publication record and be involved in a team oriented approach to science. We are a leading bio-tech firm with research facilities in New York City and can provide excellent benefits (health insurance, dental and vision plan, paid vacations and more. A high impact, high profile position with excellent opportunity for advancement Please contact Scott Shanes by phone at 609-584-8733 EXT. 218, fax CV to 609-584-9575 or E-Mail to SIS@diedremoire.com or Neurohunt@aol.com. From owner-chromosomes@net.bio.net Tue Jul 08 23:00:00 1997 Path: biosci!agate!howland.erols.net!news-peer.sprintlink.net!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!206.172.150.11!news1.bellglobal.com!sunqbc.risq.qc.ca!athena.ulaval.ca!not-for-mail From: Savita Visal Newsgroups: bionet.genome.chromosomes Subject: Looking for a Post-Doctoral position Date: Wed, 09 Jul 1997 11:21:05 +0000 Organization: Dept.Biochem, Natl Chem Lab. Pune, India Lines: 138 Message-ID: <33C3741E.C70@agora.ulaval.ca> Reply-To: bio@ems.ncl.res.in NNTP-Posting-Host: gatorg1-110.ulaval.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Dear Friends, I am advertising the Resume of my friend Dr. W Ramakrishna. He is looking for a Post-doctorate position. Savita ======--------=========------========--------=======--------========= Resume Name W.RAMA KRISHNA Address Plant Molecular Biology Unit, Division of Biochemistry National Chemical Laboratory, Pune 411 008 INDIA Research Experience: Presently working as a postdoctoral fellow at NCL, Pune, India Projects: 1) Microsatellite markers and homeobox genes and their use in phylogenetic analysis in cereals. 2)Sex determination in papaya. Educational Qualification Ph.D - DNA fingerprinting and molecular characterization of (1995) microsatellites and minisatellites in rice Laboratory Training in Molecular Biology DNA, RNA isolation, Southern and Northern hybridizations, DNA sequencing, genomic library construction, polysome isolation, preparation of wheat germ lysate, in vitro translation, RAPD & locus specific PCR, RT-PCR, DDRT-PCR, Phylogenetic analysis. References Dr. P.K. Ranjekar, Dr. Vidya S. Gupta Deputy Director & Head, Scientist, Plant Molecular Biology Unit, Plant Molecular Biology Unit, Div Biochem Sci., Div Biochem Sci., Natl Chem Lab., Nat Cheml Lab., Pune - 411 008 INDIA Pune - 411 008 INDIA email - bio@ems.ncl.res.in email - bio@ems.ncl.res.in Fax 91-0212-338234 Fax 0212-338234, Tel 91-0212-338234 Tel 0212-342779 Manuscripts 1. DNA fingerprinting in rice with oligonucleotides specific for simple repetitive DNA sequences. W. Ramakrishna, M.D. Lagu, V.S. Gupta & P.K.Ranjekar. Theoretical & Applied Genetics (1994) 88 : 402-406. 2. DNA fingerprinting detects genetic variation in rice using hypervariable DNA sequences. W. Ramakrishna, K.V.Chowdari, M.D. Lagu, V.S. Gupta & P.K.Ranjekar. Theoretical & Applied Genetics (1995) 90 : 1000-1006. 3. (CAC)5 detects DNA fingerprints and sequences homologous to gene transcripts in rice. V.S.Gupta, W.Ramakrishna, S.R.Rawat & P.K.Ranjekar. Biochemical Genetics (1994) 32 : 1/2 : 1-8. 4. DNA fingerprinting detects genetic variability in the pearl millet downy mildew pathogen (Sclerospora graminicola) using simple sequence repeats. J.G.Sastry, W.Ramakrishna, S.Sivaramakrishnan, R.P.Thakur, V.S. Gupta & P.K. Ranjekar. Theoretical & Applied Genetics (1995) 91 : 856-861. 5. DNA fingerprinting of Indian isolates of Xanthomonous oryzae pv oryzae. M.D.Rajebhonsle, K.V.Chowdari, W.Ramakrishna, S.Tamhankar, V.S.Gupta, S.S.Gnanamanickam & P.K.Ranjekar. Theoretical & Applied Genetics (In Press). Manuscripts communicated \ Underpreparation 1. Evolutionary changes associated with domestication at a (GA)n microsatellite locus in rice. W.Ramakrishna, A.M.Wadia, V.S.Gupta & P.K.Ranjekar. 2. Phylogenetic conservation and evolution of human sex determining region (SRY) gene in papaya - a dioecious plant. W.Ramakrishna, A.S.Parasnis, B.B.Dholakia, V.S.Gupta & P.K.Ranjekar (communicated to Nature Genetics) 3. Knotted1 homeobox - evolutionary significance in cereals. A.Deshpande, W.Ramakrishna, G.Mulay, V.S.Gupta & P.K.Ranjekar (to be communicated to Nature) 4. Amplification and sequencing of homeobox and microsatellites from fossils of cereals. W.Ramakrishna, A.Deshpande, A.M.Wadia, V.S.Gupta & P.K.Ranjekar (to be communicated to Nature) 5. Sex specific organization of a microsatellite in papaya. A.S.Parasnis, W.Ramakrishna, V.S.Gupta & P.K.Ranjekar (communicated to Proc.Natl.Acad.Sci.USA) 6. mRNA differential display identifies sex specific transcripts in papaya. W.Ramakrishna, A.S.Parasnis, V.S.Gupta & P.K.Ranjekar. 7. Sequence variations of rice (GATA)n microsatellite during cloning. A.M.Wadia, W.Ramakrishna, V.S.Gupta & P.K.Ranjekar. 8. Polymorphic organisation of knotted1 homeobox in cereals. A.Deshpande, W.Ramakrishna, G.Mulay, V.S.Gupta & P.K.Ranjekar 9. Microsatellite polymorphisms in somaclonal variants in rice. K.V.Choudari, W.Ramakrishna, S.Tamhankar, V.S.Gupta, & P.K.Ranjekar. 10.Molecular cloning and analysis of one member of a polymorphic family of GATA hybridizing DNA repeats in rice. A.M.Wadia, W.Ramakrishna, K.V.Chowdari, V.S.Gupta & P.K.Ranjekar. Patents Filed 1. A process for preparation of duplex polynucleotide useful for sex determination of papaya plant. A.S.Parasnis, W.Ramakrishna,V.S.Gupta & P.K.Ranjekar (Indian patent). 2. A method for early detection of sex of papaya plant using a microsatellite. A.S.Parasnis, W.Ramakrishna, V.S.Gupta & P.K.Ranjekar. (US patent- in preparation) From owner-chromosomes@net.bio.net Wed Jul 09 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.genome.chromosomes Organization: Yankee Inc. Subject: I Am Very Sorry!!! NNTP-Posting-Host: 205.139.183.55 Message-ID: <33c25553.0@nntp.kalnet.net> Date: 8 Jul 97 14:57:23 GMT Lines: 5 Path: biosci!rutgers!gatech!howland.erols.net!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.55 I would like to apologise to this newsgroup and everyone who reads this newsgroup!!! I promise never to post or send spam to this or any other newsgroup that does not pertain to my posting!!! Please accept my humble apology and again I will never post spam here again!!! Thank You!!! Andrew Schero yank714@kalnet.net From owner-chromosomes@net.bio.net Wed Jul 09 23:00:00 1997 From: randy97 Subject: http://www.love.com Newsgroups: bionet.genome.chromosomes NNTP-Posting-Host: pgh.nauticom.net Message-ID: <33c528c3.1@pgh.nauticom.net> Date: 10 Jul 97 18:24:03 GMT Lines: 9 Path: biosci!bcm.tmc.edu!cs.utexas.edu!chi-news.cic.net!newsfeed.internetmci.com!pgh.nauticom.net!pgh.nauticom.net Looking to find people in your area that enjoy the same things as this newsgroup? Check out http://www.love.com It's free, it's new, and it's awesome. Rand From owner-chromosomes@net.bio.net Thu Jul 10 23:00:00 1997 Path: biosci!rutgers!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!europa.clark.net!dispatch.news.demon.net!demon!delos.dra.hmg.gb!server1.netnews.ja.net!server5.netnews.ja.net!daresbury!not-for-mail From: mchowdhu@hgmp.mrc.ac.uk (Dr MZ Chowdhury) Newsgroups: bionet.genome.chromosomes Subject: trypsinogen Date: 11 Jul 1997 16:42:52 +0100 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5q5k9s$gnq@mserv1.dl.ac.uk> Original-To: biochrom@dl.ac.uk, gene@hgmp.mrc.ac.uk Lines: 9 Hereditary Pancreatitis is cause by mutation in the third exon of the cationic trypsinogen gene. Ehrich and group have found another mutation in the second exon.(Personal communications). They proposed that aetiology chronic pancreatitis may be due to trysinogen mutation. Fibrocalculus pancreatic diabetes (FCPD) where chronic pancreatitis and diabetes both occur. Aetiology of this disease not known. We are trying to exclude trypsinogen gene mutation in FCPD families. If any one get any new mutatin please let me know. From owner-chromosomes@net.bio.net Sat Jul 12 23:00:00 1997 Path: biosci!rutgers!gatech!newsxfer3.itd.umich.edu!infeed1.internetmci.com!newsfeed.internetmci.com!feed1.news.erols.com!dispatch.news.demon.net!demon!bullseye.news.demon.net!demon!newsgate.unisource.nl!halley.pi.net!news From: jacob Newsgroups: bionet.genome.chromosomes Subject: Asian Ladies Date: 13 Jul 1997 18:53:11 GMT Organization: World Access/Planet Internet Message-ID: <5qb86n$rtk@halley.pi.net> NNTP-Posting-Host: 145.220.210.27 Lines: 10 I've found the pefect site with nude Asian ladies. Much more than you can find in any newsgroup. http://home.pi.net/~sappie/playboy.htm From owner-chromosomes@net.bio.net Fri Jul 18 23:00:00 1997 Path: biosci!veg-physiol.med.uni-goettingen.de!HKP From: HKP@veg-physiol.med.uni-goettingen.de (Herz-Kreislauf-Physiologie) Newsgroups: bionet.genome.chromosomes Subject: reply "classical genetics question" Date: 19 Jul 1997 11:31:37 -0700 Organization: Uni Goettingen Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <33D108D9.685B@veg-physiol.med.uni-goettingen.de> Reply-To: HKP@veg-physiol.med.uni-goettingen.de NNTP-Posting-Host: net.bio.net I suppose, that this question is to be individually answered because the two genetical similarities are quite similar (sorry for my english, two beers....not american ones). Statistically two brothers should be more similar than father and son: Every brother has statistically 50% same autosomal sequences (sequences from the same chromosome) from father and mother. That equals the 100% of the paternal part of DNA for each of the brothers. The difference should come from the X/Y-chromosomes. The male members of our hypothetical family share their Y -no "plus-similarity" between father and son... The X-chromosome of our brothers is, again statistically, 50% identical (no cross over effects considered). So, brothers are more similar to each other then to their parants (both of them). From owner-chromosomes@net.bio.net Fri Jul 18 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news-peer.gsl.net!news.gsl.net!uwm.edu!msunews!harbinger.cc.monash.edu.au!not-for-mail From: "alan" Newsgroups: bionet.genome.chromosomes Subject: test Date: 19 Jul 1997 15:58:02 GMT Organization: Monash Uni Lines: 16 Message-ID: <01bc94c6$a2a09680$610ac282@monashPPP> NNTP-Posting-Host: ascend-1-33.cc.monash.edu.au Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="----=_NextPart_000_01BC951A.748828E0" Content-Transfer-Encoding: 7bit X-Newsreader: Microsoft Internet News 4.70.1155 This is a multi-part message in MIME format. ------=_NextPart_000_01BC951A.748828E0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 7bit testing ------=_NextPart_000_01BC951A.748828E0 Content-Type: text/html; charset=ISO-8859-1 Content-Transfer-Encoding: quoted-printable

testing

------=_NextPart_000_01BC951A.748828E0-- From owner-chromosomes@net.bio.net Sat Jul 19 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!dispatch.news.demon.net!demon!zetnet.co.uk!news.wisper.net!poppy.anglianet.co.uk!ttyma2.anglianet.co.uk From: "Smiffy" Newsgroups: bionet.genome.chromosomes Subject: Re: Herpies simplex Date: 20 Jul 97 09:48:02 GMT Organization: Wisper Message-ID: <01bc94fd$dd7377a0$deaca4c1@Smiffy> NNTP-Posting-Host: 193.164.172.88 X-Newsreader: Microsoft Internet News 4.70.1155 Lines: 5 Does any have or know where I can find information/papers on the Herpes simplex virus and method of its insertion of gentic material within a cell any vectors would be an added bonus. Many thanks From owner-chromosomes@net.bio.net Sat Jul 19 23:00:00 1997 Path: biosci!agate!ihnp4.ucsd.edu!munnari.OZ.AU!bunyip.cc.uq.edu.au!not-for-mail From: BJ@mailbox.uq.edu.au (Bill and Jennie) Newsgroups: bionet.genome.chromosomes Subject: X chromosome crossover, meiosis Date: Sun, 20 Jul 1997 00:21:07 GMT Organization: University of Queensland Lines: 5 Message-ID: <5qrlt2$o7p$1@nargun.cc.uq.edu.au> NNTP-Posting-Host: zzbconne.slip.cc.uq.edu.au X-Newsreader: Forte Free Agent 1.0.82 Could anyone tell me whether, during meiosis to form human ova, crossover of genetic material occurs on the arms of the X chromosomes? I`m assuming that in meiosis in the human male, this does not occur, and gametes are formed with sex chromosomes unaltered? From owner-chromosomes@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.tamu.edu!sorghum.tamu.edu!user From: ahp2343@bioch.tamu.edu (Andrew H Paterson) Newsgroups: bionet.genome.chromosomes Subject: Two postdoctoral positions in genome analysis Date: Mon, 21 Jul 1997 18:31:08 -0500 Organization: TAMU Lines: 36 Message-ID: NNTP-Posting-Host: sorghum.tamu.edu NNTP-Posting-Date: 21 Jul 1997 23:28:13 GMT TWO POSTDOCTORAL POSITIONS: GENOME ANALYSIS Postdoctoral position, Unified molecular mapping of higher plant genomes. A postdoctoral position is available (1 Sept) to develop and apply new molecular methods for the comparative molecular analysis of diverse plant taxa based on expressed genes, building upon extensive databases of prior information (cf. GENETICS 138:499, 138:829, 141:391; PNAS-USA 92:6127; SCIENCE, 269:1714-1718; NATURE GENETICS 14:380-382) and existing YAC/BAC libraries. The successful candidate will have a Ph.D. and strong publication record in genetics, molecular biology, microbiology, or allied field, proven skills in mRNA/cDNA manipulation, a high level of professional motivation, excellent written and spoken English, and strong interpersonal skills. Applicants with experience in ³cDNA selection²: methods using large DNA elements will be strongly preferred. Salary commensurate with experience, competitive benefits. To apply, mail full CV, reprints of 2-3 first-authored publications, addresses and phones of at least three professional references to Dr. Andrew Paterson, Plant Genome Mapping Laboratory, Texas A&M University, College Station, TX 77843. EMAIL ahp2343@bioch.tamu.edu. Affirmative Action/EOE. Postdoctoral position, Positional gene cloning in higher plants. A postdoctoral position is available (1 Sept, possibly earlier) to isolate well-mapped genes and QTLs that have played key roles in domestication of grasses, especially the Sh-1 gene of sorghum, using extensive databases of prior information (cf. GENETICS 138:499, 138:829, 141:391; PNAS-USA 92:6127; SCIENCE, 269:1714-1718; NATURE GENETICS 14:380-382), and existing YAC/BAC libraries. The successful candidate will have a Ph.D. and strong publication record in genetics, molecular biology, microbiology, or allied field, proven skills in cloning and analysis of large genomic DNAs, a high level of professional motivation, strong communication in English, and strong interpersonal skills. Salary commensurate with experience, competitive benefits. To apply, mail full CV, reprints of 2-3 first-authored publications, addresses and phones of at least three professional references to Dr. Andrew Paterson, Plant Genome Mapping Laboratory, Texas A&M University, College Station, TX 77843. EMAIL ahp2343@bioch.tamu.edu. Affirmative Action/EOE. From owner-chromosomes@net.bio.net Tue Jul 22 23:00:00 1997 Path: biosci!agate!howland.erols.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!mindspring!hydrant.mindspring.com!cssun.mathcs.emory.edu!cronkite.cc.uga.edu!UGA.CC.UGA.EDU!JHTSAO From: JHTSAO@UGA.CC.UGA.EDU (jhtsao) Newsgroups: bionet.genome.chromosomes Subject: MAD COW DISEASE TRANSMISSIBLE TO HUMANS VIA MEAT Date: Wed, 23 Jul 97 08:19:49 EDT Organization: University of Georgia Lines: 81 Message-ID: <17BB47529S86.JHTSAO@UGA.CC.UGA.EDU> NNTP-Posting-Host: uga.cc.uga.edu From: JHTSAO@UGA.CC.UGA.EDU (jhtsao) Path: UGA.CC.UGA.EDU!JHTSAO Newsgroups: rec.food.veg Subject: MAD COW DISEASE TRANSMISSIBLE TO HUMANS VIA MEAT Message-ID: <17BAEF23DS86.JHTSAO@UGA.CC.UGA.EDU> Date: Thu, 17 Jul 97 17:13:33 EDT Organization: University of Georgia Keywords: BSE, TSE, CREUTZFELDT-JAKOB, KURU, MADCOW The following article appeared in Flagpole Magazine, Athens, GA, on July 2, 1997, and is used by permission. Flagpole Magazine's Website is: www.flagpole. com --E-mail: flagpole@negia.net Used by permission of the editor. "Modern Cannibalism" by Maureen McGinley Forget Clive Barker, Dean Koontz and Stephen King. The most horrifying book of the year is a non-fiction account of the uncovering of a new form of disease about which you need to learn. Part of my motivation in writing this column is to get you readers to think more critically about the stuff that you eat. There are certain basic ques- tions that are worth asking about food. Who raised this? What part of the world did it come from? How was it grown? What was it fed? There are some new possible answers about meat in general and beef in particular in the book I just read that are deeply disturbing. The name of the book is _Deadly Feasts: Tracking the Secrets of a Terrifying New Plague._ Its author, Richard Rhodes, won a Pulitzer Prize for his last book on the making of the atomic bomb. In this one, he tracks the development of a new class of diseases called transmissible spongiform encephalopathy (TSE), which includes kuru in New Guinea, Creutzfeldt-Jakob disease, scrapie in sheep, transmissible mink encephalopathy (TME), and bovine spongiform encephalopathy (BSE), a.k.a. mad cow disease. All of the TSE types share some interesting features. They are one hundred percent fatal. They share many of the same traits as Alzheimer's and A.L.S., except that they are transmitted through food rather than genetically. Their distinguishing characteristic is the formation of amyloid plaques in the brain. These plaques, which show up on tissue samples when stained with Congo red dye, visually remind me of bad spots on peaches. There is no "Patient Zero," or single case to which all other cases may be traced back, as is the case with Ebola and other diseases. The incubation period, if it may be called that, can be up to 40 years in humans. Transmissible spongiform encephalopathies are not spread by germs or viruses like normal diseases. Instead, the agents of transmission are crystalline in nature. Some scientists refer to these things as prions. Prions are unique cellular disease units because there is no known way of destroying them. Tissue samples which were preserved in formaldehyde have been liquefied and and injected into animals, which then came down with the disease. Samples have also been baked in an oven at 350 degrees until they were reduced to ash and injected with the same results. Scariest of all, TSE has no inter-species transmission barrier. This means that if a cow is fed on bone and blood meal, which contains ground up mink, monkeys, cats, dogs, sheep or other cows that have died from TSE, then that cow could come down with the disease as well. If that cow is then turned into hamburger and eaten by humans, sometime in the next 40 years, the human brains may turn into sponges from TSE. Also, if the humans fertilize their roses with bone meal and happen to inhale while doing so, or put manure from infected animals on their vegetables, the same thing could happen. Richard Rhodes does a masterful job of outlining the developments in the research done in this area. He starts out describing the culture of the Fore, a tribe of Papua New Guinea, whose women and children (and sometimes men) ate their dead. Some villages ended up consisting entirely of men. From there, he traces the line through efforts to find a cure for scrapie, a lethal sheep disease, in Britain, TME in mink in Colorado, BSE in cattle in Britain and the U.S., and finally Creutzfeldt-Jakob Disease, which killed 12 young people in Britain. His description of the political battles over CJD are almost comic. In France, many cows came down with "tractor disease," where farmers would bury cows who had died of BSE, so that they would not have to destroy their herds. Blood and bone meal was hurriedly exported both legally and illegally from Britain to feed European livestock in a dizzying dance of bureaucratic illogi- cality. Since I read this book, I have heard about a woman who died of Creutzfeldt-Jakob Disease in California, and a ban on feeding imported bone and blood meal to U.S. cattle. Government officials are, of course, saying that it can't happen here. In his final book of the _2001: A Space Odyssey_ series, Arthur Clarke kills off most of the earth's population with CJD. Humans are the most idiotic of creatures at times. Like the Fore, who took to eating human remains because they asked the question, "Why should we waste this good meat?," we force livestock and other animals to become cannibals by feeding the ground-up remains of their own kind. It should come as little surprise that disastrous consequences are now occurring. Once again, Nature is trying to teach us the same old lesson: You cross the line, you pay the price. (C) Flagpole Magazine, 1997 From owner-chromosomes@net.bio.net Tue Jul 22 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!news.maxwell.syr.edu!infeed1.internetmci.com!newsfeed.internetmci.com!mindspring!hydrant.mindspring.com!cssun.mathcs.emory.edu!cronkite.cc.uga.edu!UGA.CC.UGA.EDU!JHTSAO From: JHTSAO@UGA.CC.UGA.EDU (jhtsao) Newsgroups: bionet.genome.chromosomes Subject: Re: MAD COW DISEASE TRANSMISSIBLE TO HUMANS VIA MEAT Date: Wed, 23 Jul 97 08:21:40 EDT Organization: University of Georgia Lines: 199 Message-ID: <17BB47599S86.JHTSAO@UGA.CC.UGA.EDU> NNTP-Posting-Host: uga.cc.uga.edu From: JHTSAO@UGA.CC.UGA.EDU (jhtsao) Path: UGA.CC.UGA.EDU!JHTSAO Newsgroups: rec.food.veg Subject: Re: MAD COW DISEASE TRANSMISSIBLE TO HUMANS VIA MEAT Message-ID: <17BB29619S86.JHTSAO@UGA.CC.UGA.EDU> Date: Mon, 21 Jul 97 10:39:42 EDT Organization: University of Georgia References: <17BAEF23DS86.JHTSAO@UGA.CC.UGA.EDU> <19970718174832655743@mri2.mri.montana.edu> In article <19970718174832655743@mri2.mri.montana.edu> umbjm@gemini.oscs.montana.edu (John Mercer) writes: > >jhtsao wrote: > >> The following article appeared in Flagpole Magazine, Athens, GA, on July >> 2, 1997, and is used by permission. Flagpole Magazine's Website is: >> www.flagpole. com --E-mail: flagpole@negia.net Used by permission of >> the editor. >> >> "Modern Cannibalism" by Maureen McGinley Forget Clive >> Barker, Dean Koontz and Stephen King. The most horrifying book of the >> year is a non-fiction account of the uncovering of a new form of disease >> about which you need to learn. > >Yes, you certainly do! > >> Part of my motivation in writing this column is to get you readers to >> think more critically about the stuff that you eat. There are certain >> basic ques- tions that are worth asking about food. Who raised this? >> What part of the world did it come from? How was it grown? What was it >> fed? There are some new possible answers about meat in general and beef >> in particular in the book I just read that are deeply disturbing. The >> name of the book is _Deadly Feasts: Tracking the Secrets of a Terrifying >> New Plague._ Its author, Richard Rhodes, won a Pulitzer Prize for his >> last book on the making of the atomic bomb. In this one, he tracks the >> development of a new class of diseases called transmissible spongiform >> encephalopathy (TSE), which includes kuru in New Guinea, >> Creutzfeldt-Jakob disease, scrapie in sheep, transmissible mink >> encephalopathy (TME), and bovine spongiform encephalopathy (BSE), a.k.a. >> mad cow disease. > >Rhodes never claims, as does the poster, that the disease is transmitted >by eating meat. There is no evidence to date linking vCJD to >meat-eating. Brain-eating, yes; meat-eating, no. > >See the difference? > >---snip--- > > >-- >John Mercer >Scientist >McLaughlin Research Institute Ever heard of brains for breakfast? :-) It's not vegetarian, but in some parts of the U.S. you find it on the menus of small town restaurants. I was not vegetarian until after reading Rhodes' book, but even back in those days the idea of scrambled brains as a breakfast dish offended me. Let me quote some passages from the book: p. 208 ...Dr. James Ironside is a pathologist with the British National CJD Sur- veillance Unit in Edinburgh. In September 1995, studying a brain cross section from the teenage boy who had recently died of Creutzfeldt-Jakob disease, Ironside found amyloid plaques so large that they looked under the microscope like chrysanthemum blooms. They were not confined to the cere- bellum, the pathologist determined, but spread throughout the brain. They stained for PrP. Unlike the smaller plaques of ordinary CJD, these florid plaques were surrounded by a zone of spongiform change--a destructive halo of holes. Ironside had never seen this unusual pattern of damage before, but he knew that sporadic CJD pathology varied widely from case to case. He was startled, then, when another teenage case turned up almost immediately with identical pathology. He alerted the director of the Surveillance Unit, Dr. Robert Will. Will mobilized the Unit's staff. Staff members quickly turned up six more suspect cases in young people. At first Will and Ironside thought the youthfulness of the victims might be the reason for the similari- ties in their pathology. When the physicians checked the medical literature, however, they learned that the few rare cases of CJD in people under thirty in Britain and Europe showed no such florid plaques widespread in the brain. Late in 1995, Surveillance Unit staff began traveling the country interviewing the victims' families to rule out familial CJD or iatrogenic CJD from growth hormone or surgery. By the end of February 1996, Will and Ironside knew they had an epidemiolo- gical cluster: eight cases of CJD in young people that all showed a new neuro- pathological profile of florid plaques, early loss of coordination and late dementia. Will arranged to report the ominous new finding to the Spongiform Encephalopathy Advisory Committee (SEAC), a group of scientists and physicians appointed to advise the British government on BSE. A SEAC meeting was set for March 8. Ironside opened the meeting with slides illustrating the unusual pathology. The SEAC chairman, Dr. John Pattison, remembers the moment vividly: "Before he said anything, we could see what it was. It was dramatically dif- ferent." Another SEAC member, Dr. Jeffrey Almond, recalls near panic. "The atmosphere became genuinely quite tense.Some of us were afrain that this really maybe indicated a transmission (of BSE) to humans. ...Will and Ironside now had a tenth case to report. All ten showed the same unique pathology All had what (Carleton) Gajdusek (Nobel Prize-winning researcher) would later identify as kuru plaques: the florid plaques may not have been seen in Britain and Europe, but they were diagnostic signs of kuru in Papua New Guinea....SEAC finalized its recommendations the next morning. They included destroying all British cattle over thirty months of age. p. 212. Robert Will would tell a London newspaper of these ten cases that "their brain tissue displayed a distinctive disease pattern closer to the damage inflicted on a cow's brain by BSE than the damage normal CJD inflicts on humans." At the SEAC session on March 19, senior members of the British Cabinet tried to suppress any announcement of the new variant form of CJD, arguing that the scientists might be wrong. The Secretary of State for Health, Stephen Dorrell, insisted that the public had to be told. Wednesday, March 20, speaking in the House of Commons, Dorrell informed a stunned nation that BSE had probably spread to humans from eating beef. Wouldn't you know. p.217. French and British researchers reported in June that they had success- fully infected rhesus monkeys with BSE and that the resulting lesions looked like the new-variant CJD (vCJD), with florid kuru plaques haloed with holes. "It's the first experimental argument," the French researchers told the media-- "and a very strong one--in favor of a link" between the two diseases. BSE transmission to macaques, reported the same month in _Nature_, gave further evidence of a link: "...The pathological 'signature' of the BSE agent in... kuru plaques were seen in tow macaques inoculated at the same time with sporadic CJD. Interpreting those results, a Swiss neuropathologist found "unsettling" the fact that the modest amounts of infected tissue inoculated directly into the brains of the vCJD macaques were "well within the range of brain tissue present in commercial food products for human consumption until a few years ago." We can hope, the neurolopathologist observed, "that the oral route of administration will be considerably less efficient." But the six sheep Douglass Hogg had mentioned had each been fed only five hundred milligrams of brain extract--about one-fiftieth of an ounce--and the fact that so small an oral dose had infected one with BSE did not encourage optimism. p.220. ...Just as MAFF had restricted access to its animal studies and sta- tistics, so did Robert Will of the CJD Surveillance Unit restrict information on his ongoing investigation of new suspect cases of vCJD. But when French scientists reported two additional, highly suspect French cases in spring 1996, Will refused to reciprocate wtih information on further British cases he might be investigating. Someone leaked the information: the London Sunday Times reported in June that beyond the eleven previously confirmed in Britain and one in France, the CJD Surveillance Unit was following five more suspect cases in the summer of 1996. Carleton Gajdusek called me in mid-July sounding apocalyptic. . "They don't have the least idea what caused the human cases," he told me. "It's kuru and nothing but kuru, and any species could be carrying it--dairy cows, beef cattle, pigs, chickens. They need to assess the risk and deal with it realistically. All the pigs in England fed on this meat-and-bone meal. The disease hasn't turned up in pigs only because you don't keep pigs alive for seven or eight years; they're killed after two or three years at most. When we kept pigs we'd inoculated in our laboratory for eight years, they came down with scrapie. Probably all the pigs in England are infected. And that means not only pork. It means your pigskin wallet. It means catgut surgical suture, because that's made of pig tissue. All the chickens fed on meat-and-bone meal; they're probably infected. You put that stuff in a chicken and it goes right through. A vegetarian could get it from chickenshit that they put on vegetables. It could be in tallow, in butter--how the hell am I supposed to measure infectivity in butter? No one on earth knows how to do that. These people who've come down with CJD have given blood. It's undoubtedly in the blood supply. The answer in that case is, stop giving anyone blood who doesn't really need it. Bob Will and those people don't know anything about where it came from. But I'll tell you this. If it turns up in one kid under fifteen, it's kuru. And by the way, it could be in the milk. That hasn't been excluded either." P.221....Dr. John Collinge, a neurologist at St. Mary's Hospital in London, and colleagues including James Ironside reported in _Nature_ that the molecular signature of BSE in cattle matched the molecular signature of variant CJD. p.220...With BSE, (Richard) Lacey pointed out, there was no certainty that the source of infection had been cut off; indeed, there was evidence that animals were still becoming infected with BSE and every reason to suspect that animals incubating BSE were still entering the human food supply. The consequences, he feared, could be dire for the British as they had been dire for the Fore. "If it seems that the incubation-period average for CJD in humans begins to be about twenty-five years, maybe thirty years," he told me grimly, "then the peak human epidemic will come around the year 2015. If the current numbers of variant CJD cases increase by fifty percent per year compound, as they well might, that wuld take it to about two hundred thousand cases a year by then." HUMAN cases, that is: 200,000 deaths PER YEAR. p. 230...The British government, by making the wrong public-health choices, has conducted a frightening natural experiment, allowing a lethal disease agent to spread through the human food supply, exposing the entire British population. There is every reason to believe exposure is continuing, from infected beef and possibly from infected lamb and mutton as well. ...The lack of vCJD deaths outside France doesn't mean that the rest of the continent is free of human infection; the French deaths indicate merely that exposure began there earlier. Nor do the limited number of vCJD deaths identified so far offer any basis for assessing the future of the disease agent. The ease with which BSE crosses species barriers and the fact that it is easily transmissible orally in small doses suggest that it may be an exceptionally virulent strain. p.231. What about the rest of the world? If TSEs occur sporadically in animal species, as CJD does in humans, then no population anywhere in the world that eats meat is entirely free of risk. ------ Excerpts from the book _Deadly Feasts: Tracking the Secrets of a Terrifying New Plague_, by Richard Rhodes (Pulitzer Prize winning author), published by Simon and Schuster, New York, 1997. From owner-chromosomes@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!daresbury!not-for-mail From: Rifat Hamoudi Newsgroups: bionet.genome.chromosomes Subject: Type III restriction enzymes Date: 25 Jul 1997 01:48:40 +0100 Lines: 18 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5r8t58$h17@mserv1.dl.ac.uk> Reply-To: rifat@icr.ac.uk Delivery-Receipt-To: Rifat Hamoudi MIME-Version: 1.0 X-Authentication: IMSP Original-To: biochrom@dl.ac.uk Hi, Does anyone know where I can obtain Type III restriction enzymes (e.g. 25bp cutters). If you know of a place that make them I would be grateful if you could let me know. thanks. Rifat. rifat@icr.ac.uk From owner-chromosomes@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!GAES.GRIFFIN.PEACHNET.EDU!mhopkin From: mhopkin@GAES.GRIFFIN.PEACHNET.EDU ("Mark Hopkins") Newsgroups: bionet.genome.chromosomes Subject: unsubscribe Date: 25 Jul 1997 05:05:34 -0700 Organization: Plant Genetic Resources Conservation Unit Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707251204.IAA09824@gaes.griffin.peachnet.edu> NNTP-Posting-Host: net.bio.net To whom it may concern; Please unsubscribe. Thank you. Mark From owner-chromosomes@net.bio.net Sat Jul 26 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.genome.chromosomes Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 27 Jul 1997 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707270900.CAA01992@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-chromosomes@net.bio.net Sat Jul 26 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!europa.clark.net!4.1.16.34!cpk-news-hub1.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news.alt.net!news1.alt.net!huron.eel.ufl.edu!usenet.ece.ufl.edu!freenet2.afn.org!afn50752 From: Danny Oakes Newsgroups: bionet.cellbiol,bionet.genome.chromosomes,bionet.molbio.methd-reagnts,sci.bio.botany,sci.bio.misc,sci.agriculture,sci.environment,rec.gardens,rec.gardens.edible,rec.gardens.orchids Subject: plant cloning wheel w/testtubes ? Date: Sun, 27 Jul 1997 04:29:13 -0400 Lines: 60 Message-ID: Reply-To: Danny Oakes NNTP-Posting-Host: freenet2.afn.org Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.cellbiol:7838 bionet.genome.chromosomes:1760 First, let me apologize for my cross-posting. I don't usually do this; but, I really would like info on the following: Did anyone else see this and can any of you tell me where to get more info on the following ? Several years ago, I saw a TV show news or documentary that showed some group of people creating many identical plants from one plant. From the looks of the room, I assume that this was all taking place in a university environment or a very small company startup operation. They took a little tiny sliver(?) of the parent plant. They placed the sliver in a test tube with a fairly clear liquid that filled the tube to around half full and I believe they placed a stopper in the top of the tube. The tubes were then inserted into holes in a round circular piece of plywood(?) disk, about 1/2 inch thick and about 36 to 48 inches in diameter. This disk was rotating at a very slow speed, about one revolution per minute. The plane of the rotating disk was angled up from a horizontal plane, at an angle of approximately 60 to maybe 75 degrees. I also assume there was some grow lights involved or maybe the assembly was placed outside for light source energy. The plant sliver gradually developed roots in the liquid medium while on the rotating disk. The program implied and showed that each of small beginning plants were about 3 inches long from root tip to plant top. I'm not quite sure how they maintained the point that I would call (due to my plant ignorance) the "ground level" or maybe "trunk/root boundary" of the plant. I would like to find out more about the entire process. I would especially like to find out what the clear liquid was, in the test tube. Can anyone help ??? Please e-mail me directly or call me if you are local. If you need any engineering-type questions answered, please feel free to ask. I am currently un-employed ad have plenty of time on my hands due mainly to age descrimination (53yo), arthritis and diabetes. I am trying to find something to do with my time. I also have another idea about how to grow minature plants of any type of plants using a computer controlled green house using my own software. If anyone local to me has a desire to get involved with this idea, I think we could make a lot of money. This paragraph has nothing to do with the request for information asked for above. Thanks in advance. Danny Oakes licensed professional engineer in the state of Florida Gainesville, Florida (352) 495-3371 day or night From owner-chromosomes@net.bio.net Mon Jul 28 23:00:00 1997 Path: biosci!daresbury!is.bbsrc.ac.uk!news From: David Hills Newsgroups: bionet.genome.chromosomes Subject: PROPORTION OF CHROMS. SEQUENCED Date: 29 Jul 1997 11:12:55 GMT Organization: Roslin Institute (Edinburgh) Lines: 4 Message-ID: <5rkj7n$l1p@is.bbsrc.ac.uk> NNTP-Posting-Host: pc0616.ri.bbsrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Where can I find out what and how much of the human chromosomes have been sequenced? Dave From owner-chromosomes@net.bio.net Wed Jul 30 23:00:00 1997 Path: biosci!KUCCNX.KOREA.AC.KR!skmgl From: skmgl@KUCCNX.KOREA.AC.KR (skmgl) Newsgroups: bionet.genome.chromosomes Subject: one or two hybridization?? Date: 30 Jul 1997 23:02:42 -0700 Organization: KOREA UNIV. graduated school of Biotechnology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <33E02ACE.C15@kuccnx.korea.ac.kr> Reply-To: skmgl@kuccnx.korea.ac.kr NNTP-Posting-Host: net.bio.net hi, I don't know about one or two hybridization system. (It's principles and methods et al...) Please tell me informations. regards From owner-chromosomes@net.bio.net Thu Jul 31 23:00:00 1997 Newsgroups: bionet.genome.chromosomes From: katie@ifm.mh-hannover.de (Katrin Ausmeier) Subject: mouse genome and markers Content-Type: text/plain; charset=ISO-8859-1 Message-ID: Sender: news@tyr.mhrz.mh-hannover.de (NetNews @ MHRZ/MHH) Content-Transfer-Encoding: 8bit Organization: Inst. Molecular Biology X-Newsreader: Yet Another NewsWatcher 2.4.0 Mime-Version: 1.0 Date: Fri, 1 Aug 1997 16:48:37 GMT Lines: 11 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.apfel.de!fu-berlin.de!uniol!news.rz.uni-hildesheim.de!tyr.mhrz.mh-hannover.de!MHH!katie Hi, I'm a student in a lab in germany working on a mouse mutation called Fused toes. The mutation is characterized by a large deletion of the genomic DNA on chromosome 8 in region D. I would like to determine the size of the deleted region by checking some genomic markers. (The maximum size of the deletion should be 1000 kb, because I could not observe changes in the chromosome structure.) Is there anyone who can explain me which markers I could use in my experiments??? Regards Katrin Ausmeier