From owner-chromosomes@net.bio.net Sun Aug 02 23:00:00 1998 Path: biosci!SLIP.NET!grizzly From: grizzly@SLIP.NET (Michael Sherrell) Newsgroups: bionet.genome.chromosomes Subject: Sequencers/synthesizers Date: 3 Aug 1998 16:05:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BDBEF8.2CBD1D00@oak-pm1-50-178.dialup.slip.net> NNTP-Posting-Host: net.bio.net I have a number of peptide and oligo synthesizers and sequencers for = sale: Licor 4200S-2 two-laser Sequencer, 4 mos. old Licor 4000L sequencer, ~$30K PerSeptive 9050, ~$6K PerSeptive Expedite 8909, <=3D $14K ABI 394, rebuilt, warranteed, Oligonet-ready, ~$12K ABI 394, rebuilt, warranteed, non-Oligonet, ~$11K ABI 391, rebuilt, warranteed, $7K ABI 430, rebuilt, warranteed, ~$12K ABI 431, ~$14K ABI 432 Synergy ABI 433, rebuilt by ABI, warranteed, < $40K ABI 373 classic, $10,500 ABI 373 stretch, 5-filter, 36-lane, $29K ABI 477, <=3D $10K ABI Catalyst 800 ABI 120, 130, $2.5K [We rebuild valve blocks for ABI 430, 431 and 39x @ $65/port, 90-day = warranty] also: LC-MS: Finnigan TSQ 7000, ~$200K Fisons VG 2000, <$100K Finnigan MAT 900, <$50K Finnigan MAT 90, ~$45K =09 MALDI-TOF: HP, masses to 500 KDa, lightly used, <=3D$60K Finnigan Laser MAT 2000, <$40K Other expensive hi-tech items: Bio-Dot sub-microliter 8-channel aspirate/dispense system (typically = 96-well microplate source, glass slide, microwell plate or membrane = target), < 1 year old Molecular Devices 445SI-MAC Phosphorimager, ~ 3 years old, currently = operating and under maintenance contact I also have available a few other synthesizers and sequencers and a = wide selection of HPLCs, mass specs, robots and other lab instruments. Please contact me to discuss any of these items, or if you have any = items you might like to sell. Michael Sherrell Grizzly Analytical (USA) 707 887 2919/fax 707 887 9834 www.grizzlyanalytical.com From owner-chromosomes@net.bio.net Mon Aug 03 23:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news1.best.com!peerfeed.ncal.verio.net!Supernews60!supernews.com!uunet!in1.uu.net!news.tin.it!news.tin.it!not-for-mail From: "ANMIC" Newsgroups: bionet.genome.chromosomes Subject: Mild fixation Date: 3 Aug 1998 09:17:40 GMT Organization: TIN Lines: 5 Message-ID: <01bdbebf$dff2bc60$0dabd8d4@ppichier> NNTP-Posting-Host: a-vt3-14.tin.it X-Newsreader: Microsoft Internet News 4.70.1161 I'm Pietro and I'm working in Cytogenetics field, I'm looking for a protocol of mild fixation for mammalian cells in order to use the sample for immunocytochemistry. Is there anyone who can help me? Thanks From owner-chromosomes@net.bio.net Tue Aug 04 23:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!news1.best.com!newsxfer3.itd.umich.edu!nntp.news.xara.net!xara.net!server6.netnews.ja.net!singer.cent.gla.ac.uk!usenet From: flmv1v@pop-server.cent.gla.ac.uk Newsgroups: bionet.genome.chromosomes,bionet.general Subject: mouse and cat Date: Wed, 05 Aug 1998 08:28:56 +0100 Organization: University of Glasgow Lines: 6 Message-ID: <35C809B8.FF79BCCD@pop-server.cent.gla.ac.uk> NNTP-Posting-Host: ewb1v.garscube.gla.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (WinNT; I) Xref: biosci bionet.genome.chromosomes:2138 bionet.general:30522 I need to look at chromosome X in a cat. However I can only put my hand on chromosome paint for mouse. How closely related are the X chromosome in cat and mouse? Thanks for any help From owner-chromosomes@net.bio.net Mon Aug 10 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!news.maxwell.syr.edu!newsfeed.nacamar.de!dispose.news.demon.net!demon!ayres.ftech.net!news.ftech.net!colt.net!Pollux.Teleglobe.net!server-b.cs.interbusiness.it!not-for-mail From: Tiziana Gobbi Newsgroups: bionet.genome.chromosomes Subject: Liver Developm,Gene Reg. & Disease Congress Date: Tue, 11 Aug 1998 17:06:23 +0000 Organization: IRBM P. Angeletti Message-ID: <35D07A03.7E576E70@irbm.it> Reply-To: gobbi@irbm.it NNTP-Posting-Host: 195.103.47.194 Mime-Version: 1.0 Content-Type: multipart/mixed; boundary="------------BF1202A80E18E2B820D5158F" X-Mailer: Mozilla 4.01 (Macintosh; I; PPC) X-Priority: 3 (Normal) Lines: 79 This is a multi-part message in MIME format. --------------BF1202A80E18E2B820D5158F Content-Type: text/plain; charset=iso-8859-1; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 8bit 8th Biennial International Congress on: LIVER DEVELOPMENT, GENE REGULATION AND DISEASE Convention Center at Palazzo del Popolo, Orvieto, Italy JUNE 2-5, 1999 Scientific Organizers: G. Ciliberto (IRBM P. Angeletti) and M. Tripodi (Università di Roma “La Sapienza) Scientific Program: the scientific program will include Plenary Lectures, Sessions and Posters Sessions and Speakers: Signalling and Transcription: R. H. Costa (Chicago), A. Kahn (Paris), M. Karin (La Jolla), V. Poli (Dundee), M.Yaniv (Paris) Control of Cell Cycle & Liver Mass Homeostasis: G. J. Darlington (Houston), A.M. Diehl (Baltimore), N. Fausto (Seattle), G. K. Michalopoulos (Pittsburgh), R. Taub (Philadelphia), M. Tripodi (Rome) Organogenesis: R. Di Lauro (Naples), S.A. Duncan (Milwaukee), P. Gruss (Göttingen), M. C. Weiss (Paris), K. Zaret (Providence) Metabolism: R. W. Hanson (Cleveland), H. Towle (Minneapolis) Molecular Biology and Immunology of Viral Hepatitis: R. De Francesco (Rome), F.V. Chisari (La Jolla), A. Nicosia (Rome) Gene Therapy: G. Ciliberto (Rome), M. Kay (Seattle), F. Mavilio (Milan), I. Verma (La Jolla) Deadline for Applications, Accommodation and Poster Presentations: March 2nd, 1999 Registration Fee: 900.000 Italian Lira, which include: attendance to the Congress, conference pack, welcome cocktail and welcome buffet dinner, buffet luncheons, coffee-breaks, and bus transportation to and from Rome airport For further information, depending on the specific needs, please call either: the Organizing Secretariat CITTA’ IDEALE tel. and Fax: +39-075-8743 643 / 8748 439 e-mail: ideale@tour-in-italy.com http://www.tour-in-italy.com or the Scientific Secretariat IRBM P. ANGELETTI Mrs. Tiziana Gobbi tel. +39-06-91093 655 Fax +39-06-91093 654 e-mail: liverorvieto@irbm.it http://www.irbm.it --------------BF1202A80E18E2B820D5158F Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Tiziana Gobbi Content-Disposition: attachment; filename="vcard.vcf" begin: vcard fn: Tiziana Gobbi n: Gobbi;Tiziana org: IRBM P. ANGELETTI adr: ;;Via Pontina km. 30,600;Pomezia;ROME, Italy;00040; email;internet: gobbi@irbm.it title: Coord. Scientific Secretariat tel;work: +39 - 6 - 910 93 655 tel;fax: +39 - 6 - 910 93 654 x-mozilla-cpt: www.irbm.it;0 x-mozilla-html: FALSE end: vcard --------------BF1202A80E18E2B820D5158F-- From owner-chromosomes@net.bio.net Sun Aug 16 23:00:00 1998 Path: biosci!ETS.LIMAPERU.NET!esly From: esly@ETS.LIMAPERU.NET ("Esly Anderson Motta") Newsgroups: bionet.genome.chromosomes Subject: PCR for CML Date: 17 Aug 1998 08:40:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <000801bdc967$3cdbe3a0$33400ac8@limanet.ets> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. ------=_NextPart_000_0005_01BDC93D.47955940 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable we=B4re a group of pathologist, in Lima, Peru, some colegues told us = that you have information about nucleotides sequences.=20 We need information about nucleotides sequences for PCR of Chronic = myeloid leukemia Do you have this information? =20 Abelardo Arias Velasquez, MD. E mail ariasv@ets.limaperu.net docs2@ets.limaperu.net ------=_NextPart_000_0005_01BDC93D.47955940 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
we´re a group of pathologist, = in Lima,=20 Peru, some colegues told us that you have information about nucleotides=20 sequences.
We need information about = nucleotides sequences=20 for PCR of Chronic myeloid leukemia
Do you have this = information?
          &nbs= p;    =20
    Abelardo Arias = Velasquez,=20 MD.
    E = mail   ariasv@ets.limaperu.net
          &nbs= p;    =20 docs2@ets.limaperu.net<= /FONT>
 
------=_NextPart_000_0005_01BDC93D.47955940-- From owner-chromosomes@net.bio.net Tue Aug 18 23:00:00 1998 Path: biosci!daresbury!not-for-mail From: Rifat Hamoudi Newsgroups: bionet.genome.chromosomes Subject: Robot for sale Date: 19 Aug 1998 02:03:39 +0100 Lines: 41 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <6rd89b$dlf@mserv1.dl.ac.uk> Delivery-Receipt-To: Rifat Hamoudi X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by mserv1.dl.ac.uk id CAA13992 MIME-Version: 1.0 X-Authentication: IMSP Original-To: biochrom@dl.ac.uk, methods@dl.ac.uk Dear All, We are trying to get rid of Amersham Vistra Robot because we don't have space for it anymore. The robot is good for the following applications : 1) Automated DNA mini preps (it does upto 32 minipreps) 2) Flourescent DNA Sequencing 3) Radioactive DNA Sequencing 4) Automated PCR setup 5) PCR cleanups It comes with computer, manuals and is in very good condition and fully functional. It was bought for £50,000 in 1995 but will consider any reasonable offer (even below £10,000). If you are interested please email me with your price. Thanks ====================================== Rifat Hamoudi, Cancer Gene Cloning Laboratory Manager, Haddow Laboratories, Institute of Cancer Research, 15, Cotswold Road, Sutton, SM2 5NG. Tel : 0181-643 8901 ext. 4658 Fax : 0181-770 7290 Email : rifat@icr.ac.uk ======================================= From owner-chromosomes@net.bio.net Tue Aug 18 23:00:00 1998 Path: biosci!daresbury!not-for-mail From: Newsgroups: bionet.genome.chromosomes Subject: m1419@hotmail.com Date: 19 Aug 1998 04:45:15 +0100 Lines: 58 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <6rdhob$k49@mserv1.dl.ac.uk> Original-To: Friend@public.com BENCHMARK PRINT SUPPLY 1091 REDSTONE LANE ATLANTA GA 30338 CALL----> 770-399-0953 FOR TONER SUPPLIES ORDERS/PRICING ONLY CALL----> 770-399-5505 CUSTOMER SERVICE/SUPPORT ISSUES CALL----> 770-399-5614 E-MAIL REMOVAL COMPLAINTS LINE OR BY E-MAIL TO -----> http://remove-list.com OUR LASER PRINTER/FAX/COPIER TONER CARTRIDGE PRICES NOW AS LOW AS $39 & UP. SPECIALS WEEKLY ON ALL LASER PRINTER SUPPLIES. WE CARRY MOST ALL LASER PRINTER CARTRIDGES, FAX SUPPLIES AND COPIER TONERS AT WAREHOUSE PRICES INCLUDING: HEWLETT PACKARD SERIES 2/3/4/2P/4P/5P/4L/5L/3SI/4SI/5SI IBM/LEXMARK OPTRA SERIES 4019/4029/4039/4049/4059 EPSON SERIES 2/1100/1500/6000/7000/8000 NEC SERIES 90/95 CANON COPIER PC SERIES INCLUDING 3/6RE/7/11/320/720/10/20/25 ETC... HP FAX SERIES 700/720/5000/7000/FX1/FX2/FX3/FX4/FX5 CANON FAX ALL MODELS PRICES CHANGE WEEKLY PLEASE CALL TO GET THE MOST RECENT PRICING/AVAILABILTY AND SPECIALS OF THE WEEK!!!! PLEASE PLEASE PLEASE MAKE NOTE OF THE FOLLOWING BEFORE YOU CALL: -----> WE DO NOT HAVE CATALOGS OR PRICE LISTS BECAUSE OUR PRICES CHANGE WEEKLY!! -----> WE DO NOT FAX QUOTES OR PRICES BECAUSE OUR ORDER LINE IS NOT SET UP TO DO THAT -----> WE DO NOT SELL TO RESELLERS OR BUY FROM DISTRIBUTERS -----> WE DO NOT CARRY : BROTHER -MINOLTA-KYOSERA- PANASONIC - XEROX - FUJITSU - OKIDATA - SHARP!! -----> WE DO NOT CARRY ANY COLOR PRINTER SUPPLIES!!!!! -----> WE DO NOT CARRY DESKJET/INKJET OR BUBBLEJET SUPPLIES!!!! NEW,NEW,NEW $10 MAIL IN REBATE ON SELECTED CARTRIDGES SIMPLY ORDER YOUR NEW CARTRIDGE, WHEN IT ARRIVES MAIL BACK YOUR REBATE CERTIFICATE WITH ANY USED CARTRIDGE FOR INSTANT CREDIT WE ACCEPT ALL MAJOR CREDIT CARDS OR COD ORDERS CORPORATE ACCOUNTS AVAILABLE WITH APPROVED CREDIT ALL PACKAGES SHIPPED UPS GROUND UNLESS SPECIFIED OTHERWISE From owner-chromosomes@net.bio.net Tue Aug 18 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!howland.erols.net!fu-berlin.de!tburmei.dialup.fu-berlin.DE!not-for-mail From: tbu@gmx.net (Thomas Burmeister) Newsgroups: bionet.genome.chromosomes Subject: Re: PCR for CML Date: Wed, 19 Aug 1998 21:23:00 +0200 Organization: Freie Universitaet Berlin Lines: 47 Distribution: world Message-ID: References: <000801bdc967$3cdbe3a0$33400ac8@limanet.ets> NNTP-Posting-Host: tburmei.dialup.fu-berlin.de (130.133.216.94) Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Access: 16 17 18 X-Trace: fu-berlin.de 903554102 21519 (none) 130.133.216.94 In article <000801bdc967$3cdbe3a0$33400ac8@limanet.ets>, esly@ETS.LIMAPERU.NET ("Esly Anderson Motta") wrote: >we=B4re a group of pathologist, in Lima, Peru, some colegues told us = >that you have information about nucleotides sequences.=20 >We need information about nucleotides sequences for PCR of Chronic = >myeloid leukemia >Do you have this information? > =20 > Abelardo Arias Velasquez, MD. > E mail ariasv@ets.limaperu.net > docs2@ets.limaperu.net Dear Abelardo Arias Velasquez, several BCR/ABL-primer pairs are used by laboratories concerened with this kind of diagnostics. We are currently organizing an interlaboratory test for quality asurement in Germany and I was really surprised how many different (PCR-based) test systems are in use. Here are two literature hints: (Single PCR:) Cross NC, Melo JV, Feng L, Goldman JM (1994) An optimized multiplex polymerase chain reaction (PCR) for detection of BCR-ABL fusion mRNAs in haematological disorders. Leukemia 1994 Jan;8(1):186-189 MEDLINE-Abstract: http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8289486&form=6&db=m&Dopt=b (Nested PCR:) Maurer J, Janssen JW, Thiel E, van Denderen J, Ludwig WD, Aydemir U, Heinze B, Fonatsch C, Harbott J, Reiter A, et al (1991) Detection of chimeric BCR-ABL genes in acute lymphoblastic leukaemia by the polymerase chain reaction. Lancet May 4;337(8749):1055-1058 MEDLINE-Abstract: http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=1673492&form=6&db=m&Dopt=b (We are currently using a modified version of the second method.) Good luck ! T. Burmeister -- Dr. Thomas Burmeister Free University Berlin Germany From owner-chromosomes@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!cyclone.news.idirect.com!island.idirect.com!news1.bellglobal.com!198.235.216.4.POSTED!not-for-mail From: "Kathy" Newsgroups: bionet.genome.chromosomes References: <6r036h$9df@nnews.vitinc.com> Subject: HELP Lines: 16 X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Message-ID: Date: Thu, 20 Aug 1998 16:37:08 GMT NNTP-Posting-Host: 204.138.179.141 NNTP-Posting-Date: Thu, 20 Aug 1998 12:37:08 EDT Organization: Bell Solutions Hello I don't know if I am in the right area or not but maybe someone can point me in the right direction... I have been trying to find any information that I can for a friend who has a daughter with chromosome 2 & 11 transversed.. The daughter is having seizures and wanted to find out all we could about Vitamin B6 Therapy and also Intense Oxygen Therapy.... Her pediatrician is treating the seizures with Phenobarbital... Now I do not understand a whole lot of medical terms but we are looking for information in layman's terms that maybe could help Mom understand her daughters illness more... If there is anyone out there that can point me in the right direction to find these things out I would greatly appreciate it... thanks From owner-chromosomes@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!BCCANCER.BC.CA!AIshkani From: AIshkani@BCCANCER.BC.CA (Adrian Ishkanian) Newsgroups: bionet.genome.chromosomes Subject: unique human dna sequence Date: 19 Aug 1998 19:51:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I'm looking for a unique stretch of human dna that I can use for an internal control in PCR reactions. Does anyone have any ideas? From owner-chromosomes@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!news.maxwell.syr.edu!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey03.news.aol.com!not-for-mail From: ehorvath35@aol.com (EHorvath35) Newsgroups: bionet.genome.chromosomes Subject: Fragile X Syndrome Lines: 4 Message-ID: <1998082012384900.IAA26228@ladder03.news.aol.com> NNTP-Posting-Host: ladder03.news.aol.com X-Admin: news@aol.com Date: 20 Aug 1998 12:38:49 GMT Organization: AOL http://www.aol.com Can anyone tell me if the methylation status in a fragile x person can change over time....or does it always stay the same? Thank you, From owner-chromosomes@net.bio.net Mon Aug 24 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!sunqbc.risq.qc.ca!newspeer.monmouth.com!news.ultranet.com!not-for-mail From: "Marc Andelman" Newsgroups: bionet.genome.chromosomes Subject: genomics position Date: Tue, 25 Aug 1998 16:52:29 -0700 Organization: UltraNet Communications , an RCN Company http://www.ultranet.com/ Lines: 20 Message-ID: <6rv7no$7k6$1@strato.ultra.net> NNTP-Posting-Host: d16.dial-4.cmb.ma.ultra.net X-Complaints-To: abuse@ultra.net X-Ultra-Time: 25 Aug 1998 20:44:40 GMT X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Biosource is the oldest recruitment firm in the biotech industry and an employer paid fee service. We currently have a client seeking to hire people to manage positional cloning projects. Experience such as with BACs, YACs, and contigs is required. This is an extremely well funded company outside of the United States. Interested people please E-mail Marc Andelman at drgonfly@ultranet.com, or call 508 853 8803, or FAX 508 853 8773. Unless specifically asked, please allow me only to get back in touch if this or some other position is a dead ringer for a person's and the client's interest. I will of course call first before sending anyone's information along. Thank you, Marc Andelman From owner-chromosomes@net.bio.net Mon Aug 24 23:00:00 1998 Newsgroups: bionet.genome.chromosomes,bionet.molbio.gdb,bionet.molbio.gene-linkage Path: biosci!ihnp4.ucsd.edu!sdd.hp.com!usc!howland.erols.net!newsfeed.internetmci.com!194.125.133.14!news.indigo.ie!HEAnet!server5.netnews.ja.net!leeds.ac.uk!news From: t.j.keen@leeds.ac.uk (T. Jeffrey Keen) Subject: 3-hydroxyisobutyrate dehydrogenase Message-ID: NNTP-Posting-Host: sjuh-pc146.leeds.ac.uk Organization: University of Leeds Date: Tue, 25 Aug 1998 13:30:48 +0100 (BST) X-Newsreader: MT-NewsWatcher 2.3.5 Lines: 9 Xref: biosci bionet.genome.chromosomes:2148 bionet.molbio.gdb:886 bionet.molbio.gene-linkage:1873 Does anyone know the human chromosomal location of 3-hydroxyisobutyrate dehydrogenase? Nobody appears to have sequenced a full length cDNA clone, though there is a large UniGene cluster which clearly corresponds to the gene. http://inhouse.ncbi.nlm.nih.gov/cgi-bin/UniGene/clust?ORG=Hs&CID=102521 Thanks