From owner-chromosomes@net.bio.net Sun Nov 01 22:00:00 1998
Path: biosci!DIHT.COM.SG!tuujoelo84
From: tuujoelo84@DIHT.COM.SG (frezxery)
Newsgroups: bionet.genome.chromosomes
Subject: Reach your internet clients - before competition
Date: 2 Nov 1998 06:57:47 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 31
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199811023201ZAA41004@frebrequaz.ghyis2.cnb.uam.es>
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From owner-chromosomes@net.bio.net Tue Nov 03 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!newsfeed.gamma.ru!Gamma.RU!newsfeed.wirehub.nl!bullseye.news.demon.net!demon!news.demon.co.uk!demon!gfutures.demon.co.uk!gfutures
From: Green Futures <gfutures@gfutures.demon.co.yuk>
Newsgroups: bionet.genome.chromosomes
Subject: GMO DISASTER
Date: Wed, 4 Nov 1998 00:10:58 +0000
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From owner-chromosomes@net.bio.net Wed Nov 04 22:00:00 1998
Path: biosci!news.stanford.edu!logbridge.uoregon.edu!europa.clark.net!194.162.162.196!newsfeed.nacamar.de!ayres.ftech.net!news.ftech.net!colt.net!demeter.clara.net!news.clara.net!easynet-tele!easynet-uko!news.ukonline.co.uk!not-for-mail
From: "Andrew Specterman" <a.specterman@ukonline.co.uk>
Newsgroups: bionet.genome.chromosomes
Subject: Re: number of Chromosomes in an Ape, Monkey, and Chimpanzee
Date: Thu, 5 Nov 1998 18:58:18 -0000
Organization: [posted via] UK Online Ltd
Lines: 17
Message-ID: <71tbm6$mo4$1@morse.news.easynet.net>
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I think you will find the following useful

Chimpanzee has 48 pairs
Rhesus Monkey has 21 pairs
can't find ape in my genetics textbook sorry.

Andrew
a.specterman@ukonline.co.uk

Noah Davids wrote in message <01BE03E8.544BC3A0.ndav@primenet.com>...
>The question of the number of chromosomes that apes, monkeys, and
>chimpanzees has was raised during some random conversations. Does anyone
>have an answer or know where I can look? Please respond via E-mail to
>ndav@primenet.com.
>



From owner-chromosomes@net.bio.net Thu Nov 05 22:00:00 1998
Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cam-news-feed2.bbnplanet.com!news.gtei.net!news.shore.net!uunet!in4.uu.net!stalker.oem.net!not-for-mail
From: "Sean" <biostorm@biostorm.com>
Newsgroups: bionet.genome.chromosomes
Subject: New karyotyping
Date: Fri, 6 Nov 1998 11:08:44 -0500
Organization: oemnetworks.com
Lines: 76
Message-ID: <364274ea.0@rboc1.prime-x.net>
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Just to make others aware .  Biostorm a new program available: karyotpying
prrogram --
E:\ads\vt-katyo
Color-Monochrome, (FISH) Automatic Karyotyping  of Chromosomes & Cytogenetic
Analysis

The program performs automatic chromosome analysis of acquired color or
monochrome metaphase images from camera installed on microscope (light or
fluorescence), scanner, disk, clipboard, files in BMP, .PCX, .GIF, .TIF
formats, etc.


Automated Karyotyping & Cytogenetic analysis of monochrome image of
metaphase.  AABI has integrating cooled CCD video camera of high sensitivity
0.000001 Lux.  Standard Camera is 0.5 Lux

It moves the separately lying chromosomes, remove artifacts, draw axis and
centromeres on the chromosomes, measure them and get data tables, mark
chromosome numbers, get karyotypes in manual or automatic mode and make
corrections if necessary. For complicated cases there is an option to
analyze the chromosome idiograms with a possibility of their editing,
averaging and comparing. The original images, karyotypes, tables, idiograms
can be printed and used for reports and publications.

Image Analysis System with AABI-Karyo software package facilitates the
cytogenetic researches making them more effective, up-to-date, interesting,
free from routine work. The medicine genetics departments, prenatal
diagnostics laboratories of Obstetrics and Gynocology, and molecular
genetics
Laboratories use this program for diagnostics of the complicated cases,
archiving, storing information in Image Data Base, and facilitation of data
exchange through e-mail and Internet.

The program is also used for animal and plant chromosomes of livestock
genetics and breeding Labs.

Requirements Windows 3.1 or better and 2Mb of disk space. It supports TWAIN
and MCI devices.

All information on the image is linked: chromosome - data - karyogram.  Just
click on a chromosome
and get all information on measured parameters for that chromosome.
Main Functions:
   --Acquire metaphase images with TV-camera, scanner, reading from disk or
from data base
  --Consolidate the image by  moving chromosomes and image enhancement..
  --Edit the images, remove artifacts, print texts, annotate, etc.
  --Automatic arrangement of the chromosomes in karyograms with simultaneous
bands enhancement.
  --Edit karyograms.
  --Display chromosome parameters in a table.
  --Measure the chromosome parts.
  --Perform density profiles for chromosomes.
  --Export of the images and karyograms to a data base
  --Print images and analysis results.

  Automatic Karyotyping  & Cytogenetic analysis of multicolor fluorescence
In Situ Hybridization, FISH.
AABI has 3-chip color CCD video camera with frame grabber


Archiving                                 Morphology
Data basing

Image Data Base can be displayed as Gallery or Blank. Blank contains the
image and supporting information (tables, graphs, histograms, text, images).
In Gallery: all Data Base images are displayed.


----------------------------------------------------------------------------
----------------------------------------------------------------------------
-




From owner-chromosomes@net.bio.net Thu Nov 05 22:00:00 1998
Path: biosci!success600.com!success600
From: success600@success600.com
Newsgroups: bionet.genome.chromosomes
Subject: Fire the Boss,  Break the Alarm Clock
Date: 5 Nov 1998 20:42:57 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 16
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <19981105232439.XAA26871@success600.com>
NNTP-Posting-Host: net.bio.net


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From owner-chromosomes@net.bio.net Sat Nov 07 22:00:00 1998
Path: biosci!AOL.COM!Cutegirl21
From: Cutegirl21@AOL.COM
Newsgroups: bionet.genome.chromosomes
Subject: WANT A NEW CELL PHONE?  YOU'RE APPROVED!
Date: 8 Nov 1998 05:34:54 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 42
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <7609c051.36459c6e@aol.com>
NNTP-Posting-Host: net.bio.net

This is a multi-part message in MIME format.

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Content-type: message/rfc822
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From owner-chromosomes@net.bio.net Sat Nov 07 22:00:00 1998
Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!news.maxwell.syr.edu!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail
From: capncoons2@aol.com (CapnCoons2)
Newsgroups: bionet.genome.chromosomes
Subject: Re: Sex Sex Sex Sex Sex
Lines: 2
NNTP-Posting-Host: ladder01.news.aol.com
X-Admin: news@aol.com
Date: 8 Nov 1998 01:50:32 GMT
Organization: AOL http://www.aol.com
References: <71m09n$v27$4298@duke.telepac.pt>
Message-ID: <19981107205032.00642.00003816@ng02.aol.com>

cool


From owner-chromosomes@net.bio.net Sat Nov 07 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail
From: capncoons2@aol.com (CapnCoons2)
Newsgroups: bionet.genome.chromosomes
Subject: Re: chromosomes
Lines: 1
NNTP-Posting-Host: ladder01.news.aol.com
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Date: 8 Nov 1998 01:51:02 GMT
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References: <19981101002302.16773.00001817@ng112.aol.com>
Message-ID: <19981107205102.00642.00003817@ng02.aol.com>

if you find, out tell me

From owner-chromosomes@net.bio.net Sat Nov 07 22:00:00 1998
From: "Hank Burr" <hburr@ohiohills.com>
Subject: human 2,3 translocations
Newsgroups: bionet.genome.chromosomes
Message-ID: <01be0b36$f4536c40$8333afce@mycomputer.ohiolinks.com>
X-Newsreader: Microsoft Internet News 4.70.1155
NNTP-Posting-Host: 206.175.51.131
Date: 8 Nov 1998 11:48:35 -0500
X-Trace: 8 Nov 1998 11:48:35 -0500, 206.175.51.131
Lines: 7
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!howland.erols.net!isdnet!arl-news-svc-4.compuserve.com!news-master.compuserve.com!compuserve.com!news.netwalk.com!206.175.51.131

Does anyone have information on 2,3 reciprocal translocations in humans?  I
am interested in the clinical effects and frequency of such events.

Please e-mail me, or post response to the Group.  Thank you.

Hank Burr
hburr@0hiohills.com

From owner-chromosomes@net.bio.net Wed Nov 11 22:00:00 1998
Path: biosci!IEC-NICHIBEI.OR.JP!suupueqau87
From: suupueqau87@IEC-NICHIBEI.OR.JP (Photo Transfer Specialties)
Newsgroups: bionet.genome.chromosomes
Subject: Photo Mousepads...........Great Gift Idea!
Date: 11 Nov 1998 23:57:14 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 152
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199811113745HAA23698@ChristmasGift.cl>
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From owner-chromosomes@net.bio.net Thu Nov 12 22:00:00 1998
Path: biosci!gsf.de!h.gohlke
From: h.gohlke@gsf.de (Henning Gohlke)
Newsgroups: bionet.genome.chromosomes
Subject: Sequenzing of BACs
Date: 13 Nov 1998 02:15:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 9
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <364C06A3.C4BB713@gsf.de>
NNTP-Posting-Host: net.bio.net

Hello everybody!
I am doing a positional cloning approach to identify a cataract mutaion
in mice. I have a lot of trouble with sequencing the Bacs. I am using a
modified Qiagen-Bac-Preparation. I very often get a good harvest, but I
am not always able to sequence it. Can anyone give my an advice, how I
can improve the qualtity of my Bac-Preparation.
Thank you very much
Yours Henning


From owner-chromosomes@net.bio.net Sun Nov 15 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!newsfeed.cwix.com!193.190.198.38!news.belnet.be!inf6serv.rug.ac.be!not-for-mail
From: Jyoti Kapila <jykap@gengenp.rug.ac.be>
Newsgroups: bionet.genome.chromosomes
Subject: purification of BAC
Date: Mon, 16 Nov 1998 15:43:07 +0100
Organization: University of Ghent, Belgium
Lines: 14
Message-ID: <365039FB.A4CA0832@gengenp.rug.ac.be>
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X-Accept-Language: en

I would like to purify the large DNA fragment of about 150 kb from the
BAC clone to further clone it into the required vector.  Could some one
help me with sending a protocol for purfication of the BAC fragment from
the gel for e.g.
Thanks for the help.
Jyoti
-- 
==================================================================
Jyoti Kapila 
DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Vlaams Instituut voor Biotechnologie                           VIB
mailto:jykap@gengenp.rug.ac.be    	   http://spider.rug.ac.be
==================================================================

From owner-chromosomes@net.bio.net Wed Nov 18 22:00:00 1998
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.cwix.com!204.59.152.222!news-peer.gip.net!news-raspail.gip.net!news.gsl.net!gip.net!rain.fr!wanadoo.fr!not-for-mail
From: "Christophe" <Labo.lefhe@wanadoo.fr>
Newsgroups: bionet.genome.chromosomes
Subject: Insect caryotype
Date: Tue, 17 Nov 1998 18:03:47 +0100
Organization: Labo LEFHE
Lines: 15
Message-ID: <72sa4r$bk3$1@platane.wanadoo.fr>
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Dear All,

I'm looking for cell culture protocol about coleoptera insects.

Thanks

Labo.lefhe@wanadoo.fr

Laboratoire d'entomologie faune hypogee et endogee - france -

Le directeur des recherches.

Christophe Avon



From owner-chromosomes@net.bio.net Thu Nov 19 22:00:00 1998
Path: biosci!CS.Arizona.EDU!noao!math.arizona.edu!news.Arizona.EDU!uunet!in5.uu.net!chippy.visi.com!news-out.visi.com!newspeer.monmouth.com!colt.net!Pollux.Teleglobe.net!server-b.cs.interbusiness.it!news.tin.it!not-for-mail
From: "ANMIC" <anmicvt@tin.it>
Newsgroups: bionet.cellbiol,bionet.genome.chromosomes,bionet.molbio.methds-reagnts
Subject: Info
Date: 19 Nov 1998 16:33:25 GMT
Organization: TIN
Lines: 11
Message-ID: <01be13da$2c34ab00$2babd8d4@ppichier>
NNTP-Posting-Host: a-vt3-44.tin.it
X-Newsreader: Microsoft Internet News 4.70.1161
Xref: biosci bionet.cellbiol:10860 bionet.genome.chromosomes:2214 bionet.molbio.methds-reagnts:72314

I would like to have information on RNA quantification methods and
trascription quantification methods based on non-isotopic reagents.
I'll be very grate to everyone could help me.

                                                 Pietro


Laboratory of Molecular Cytogenetics
Università della Tuscia
Italy
e-mail pichierri@iname.com

From owner-chromosomes@net.bio.net Thu Nov 19 22:00:00 1998
Path: biosci!biosci!not-for-mail
From: Philip Lijnzaad <lijnzaad@ebi.ac.uk>
Newsgroups: bionet.genome.chromosomes,bionet.software
Subject: CORBA: Request For Proposals on Genomic Maps
Date: 19 Nov 1998 20:34:25 -0800
Organization: EMBL Outstation Hinxton - European Bioinformatics Institute, UK
Lines: 39
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <u7d86mo1ff.fsf@ebi.ac.uk>
NNTP-Posting-Host: net.bio.net
Xref: biosci bionet.genome.chromosomes:2215 bionet.software:22318

Dear all, 

On behalf of the Maps work group (http://www.ebi.ac.uk/~lijnzaad/maps/) of
the OMG (http://www.omg.org/) Domain Task Force for Life Sciences Research
(http://www.omg.org/homepages/lsr/), we are pleased to announce the
availability of a Request for Proposals on Genomic Maps, which was approved
and issued last Friday at the OMG meeting in Burlingame.

The document will be available shortly as

ftp://ftp.omg.org/pub/docs/lifesci/98-11-08.doc (full version in MS Word
format; other formats will be provided as well)

There is a shorter version (without the standard OMG chapters) which will
be visible as 

ftp://ftp.omg.org/pub/docs/lifesci/98-11-07.doc

These documents can already now be obtained from the Maps work group home
page; see http://www.ebi.ac.uk/~lijnzaad/maps/. 

If you have any comments, questions or suggestions, please get involved by
subscribing to corba-maps@ebi.ac.uk; see
http://www.ebi.ac.uk/~lijnzaad/maps/mailing-list/about.html for details.

Yours kindly,

								Philip Lijnzaad

-- 
-----------------------------------------------------------------------------
Philip Lijnzaad, lijnzaad@ebi.ac.uk | European Bioinformatics Institute
+44 (0)1223 49 4639                 | Wellcome Trust Genome Campus, Hinxton
+44 (0)1223 49 4468 (fax)           | Cambridgeshire CB10 1SD,  GREAT BRITAIN
PGP fingerprint: E1 03 BF 80 94 61 B6 FC  50 3D 1F 64 40 75 FB 53





From owner-chromosomes@net.bio.net Fri Nov 20 22:00:00 1998
Message-ID: <365604FB.31731024@hotmail.com>
Date: Sat, 21 Nov 1998 01:10:36 +0100
From: Olivier Kunz <okunz@hotmail.com>
X-Mailer: Mozilla 4.5 [en]C-NECCK  (Win95; I)
X-Accept-Language: de, en
MIME-Version: 1.0
Newsgroups: bionet.genome.chromosomes
Subject: Do Twins have the same Fingerprint?
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
NNTP-Posting-Host: 130.60.130.57
X-NNTP-Posting-Host: 130.60.130.57
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Lines: 8
Path: biosci!agate!newsfeed.berkeley.edu!news-nyc.telia.net!newsfeed-zh.ip-plus.net!news-zh.switch.ch!rzunews1.unizh.ch!130.60.130.57

Hello
A little bit a funny question:
I beleve that twins don't (always) have the same fingerprint. But it is
only a suggestion. Does anybody be able to explain me why?

Regards
Olivier Kunz


From owner-chromosomes@net.bio.net Sun Nov 22 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.concentric.net!newshub.northeast.verio.net!btnet-peer!btnet!rill.news.pipex.net!pipex!server1.netnews.ja.net!hgmp.mrc.ac.uk!tin!kdai
From: David Dai <kdai@hgmp.mrc.ac.uk>
Newsgroups: bionet.genome.chromosomes
Subject: Marker to YAC contig
Date: Mon, 23 Nov 1998 17:09:36 +0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 7
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Dear all,

Could anybody give me some advice of finding YAC clones according to the
known microsatellite marker? Thanks.

David Dai


From owner-chromosomes@net.bio.net Mon Nov 23 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!newsfeed.axxsys.net!router1.news.adelphia.net!uunet!in5.uu.net!server-b.cs.interbusiness.it!news.tin.it!not-for-mail
From: I&IA <ieiamf@tin.it>
Newsgroups: bionet.genome.chromosomes
Subject: help: "hybrid dysgenesis in Drosophila".
Date: Tue, 24 Nov 1998 00:17:16 +0100
Organization: I&IA
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I would like to understand what is and how can be explaned the "hybrid
dysgenesis in Drosophila".

Thank you very much, I know you will help me...please.

Francesca.

From owner-chromosomes@net.bio.net Wed Nov 25 22:00:00 1998
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From: David Dai <kdai@hgmp.mrc.ac.uk>
Newsgroups: bionet.genome.chromosomes
Subject: Sort contigs
Date: Thu, 26 Nov 1998 09:28:09 +0000
Organization: MRC Human Genome Mapping Project Resource Centre
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Dear all,

Many thanks for all who have helped me with finding the way from markers
to YAC contigs! Here comes another question: what is the meaning of the
definition of 'singly-linked','doubly-linked' contig? Thanks again! 

David Dai


From owner-chromosomes@net.bio.net Thu Nov 26 22:00:00 1998
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.genome.chromosomes
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 27 Nov 1998 02:00:14 -0800
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(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


From owner-chromosomes@net.bio.net Thu Nov 26 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!netnews.com!newspeer1.nac.net!newsfeed.wirehub.nl!news.belnet.be!inf6serv.rug.ac.be!not-for-mail
From: "Ben Wielockx" <Ben.Wielockx@rug.ac.be>
Newsgroups: bionet.genome.chromosomes
Subject: Re: Marker to YAC contig
Date: Fri, 27 Nov 1998 17:52:38 +0100
Organization: rug
Lines: 12
Message-ID: <73ml41$n9b$1@inf6serv.rug.ac.be>
References: <Pine.GSO.3.94.981123170529.23580A-100000@tin>
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Mouse, human, Rat???????????

David Dai heeft geschreven in bericht ...
>Dear all,
>
>Could anybody give me some advice of finding YAC clones according to the
>known microsatellite marker? Thanks.
>
>David Dai
>



From owner-chromosomes@net.bio.net Thu Nov 26 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!netnews.com!newspeer1.nac.net!newsfeed.wirehub.nl!news.belnet.be!inf6serv.rug.ac.be!not-for-mail
From: "Ben Wielockx" <Ben.Wielockx@rug.ac.be>
Newsgroups: bionet.genome.chromosomes
Subject: Re: Sort contigs
Date: Fri, 27 Nov 1998 17:55:34 +0100
Organization: rug
Lines: 15
Message-ID: <73ml9h$nfh$1@inf6serv.rug.ac.be>
References: <Pine.GSO.3.94.981126091939.28575B-100000@tin>
NNTP-Posting-Host: pdln1p27.rug.ac.be
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Look at the homepage of the Jackson Laboratories
(http://www.informatics.jax.org/ and go to FAQ. There you 'll find the
answers for all your questions

David Dai heeft geschreven in bericht ...
>Dear all,
>
>Many thanks for all who have helped me with finding the way from markers
>to YAC contigs! Here comes another question: what is the meaning of the
>definition of 'singly-linked','doubly-linked' contig? Thanks again!
>
>David Dai
>



From owner-chromosomes@net.bio.net Sat Nov 28 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!sunqbc.risq.qc.ca!wesley.videotron.net!Pollux.Teleglobe.net!server-b.cs.interbusiness.it!news.tin.it!not-for-mail
From: "Colicigno Roberto" <rcolici@tin.it>
Newsgroups: bionet.genome.chromosomes
Subject: software
Date: Sun, 29 Nov 1998 15:40:17 +0100
Organization: TIN
Lines: 5
Message-ID: <73rms8$hi4$1@nslave1.tin.it>
NNTP-Posting-Host: a-ps3-3.tin.it
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sono uno studente al secondo anno di biologia in urbino(Italia)
vorrei sapere se esiste del software per simulazioni in laboratorio e se
possibile dove reperirlo.grazie



From owner-chromosomes@net.bio.net Sun Nov 29 22:00:00 1998
Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.gtei.net!newsfeed.berkeley.edu!news.maxwell.syr.edu!news-peer.gip.net!news-raspail.gip.net!news.gsl.net!gip.net!oleane!jussieu.fr!infobiogen.fr!bernot
From: bernot@lovelace.infobiogen.fr (Alain Bernot)
Newsgroups: bionet.genome.chromosomes
Subject: ribosomal genes
Date: 30 Nov 1998 17:08:40 +0100
Organization: Genethon -- Human Genome Research Centre
Lines: 7
Distribution: world
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Reply-To: bernot@lovelace.infobiogen.fr (Alain Bernot)
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does anybody know some species in which some ribosomal protein genes are 
presents several times per genome ?

thanks in advance.

alain bernot

From owner-chromosomes@net.bio.net Mon Nov 30 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!netnews.com!newspeer.monmouth.com!rill.news.pipex.net!pipex!server1.netnews.ja.net!pegasus.csx.cam.ac.uk!hgmp.mrc.ac.uk!tin!kdai
From: David Dai <kdai@hgmp.mrc.ac.uk>
Newsgroups: bionet.genome.chromosomes
Subject: Cosmid contig map?
Date: Tue, 1 Dec 1998 09:39:19 +0000
Organization: MRC Human Genome Mapping Project Resource Centre
Lines: 9
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Dear all,

As we know that the single chromosome library is available at UK HGMP
resource centre, it gives more facilities to our genomic projects. But
does anyone know if there is any ready-to-index cosmid contig map?
Chromosome 1, for example. Or PAC contig maps?

David Dai


