From owner-chromosomes@net.bio.net Tue Dec 01 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!oleane!pasteur.fr!not-for-mail
From: G3 <user@mailhost.pasteur.fr>
Newsgroups: bionet.genome.chromosomes
Subject: Re: ribosomal genes
Date: Wed, 02 Dec 1998 09:55:16 +0000
Organization: Institut Pasteur, Paris
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Alain Bernot wrote:

> does anybody know some species in which some ribosomal protein genes are
> presents several times per genome ?
> thanks in advance.
> alain bernot

In Saccharomyces cerevisiæ, some of them are duplicated (near 100% identity
between copies)
Arnaud


From owner-chromosomes@net.bio.net Thu Dec 03 22:00:00 1998
Path: biosci!YAHOO.COM!mri87492
From: mri87492@YAHOO.COM
Newsgroups: bionet.genome.chromosomes
Subject: about your site
Date: 4 Dec 1998 00:36:24 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 69
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Message-ID: <199812040832.AAA28106@net.bio.net>
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From owner-chromosomes@net.bio.net Thu Dec 03 22:00:00 1998
Path: biosci!MAILHOST.TCS.TULANE.EDU!alustig
From: alustig@MAILHOST.TCS.TULANE.EDU ("Lustig, Arthur J.")
Newsgroups: bionet.genome.chromosomes
Subject: telomeres at Tulane
Date: 4 Dec 1998 09:51:04 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 43
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NNTP-Posting-Host: net.bio.net

POSTDOCTORAL FELLOWS TO STUDY TELOMERE STRUCTURE AND FUNCTION

	A postdoctoral position is available to investigate telomere
dynamics in Saccharomyces cerevisiae in the laboratory of Dr. Arthr J.
Lustig at Tulane University Medical Center. We have had a long-standing
fascination in telomere structure and function and are currently focusing
on two broad areas:

	1) THE MECHANISM OF TELOMERIC SILENCING
	RECENT REFERENCES
	Park et al., Sir3p Domains Involved in the Initiation of Telomeric
Silencing in Saccharomyces cerevisiae. Genetics 150:  977-986 (1998);
	Lustig, A., Mechamisms of silencing in Saccharomyces cerevisiae.
Current Opinions in Gentetics and Development 8: 233-239 (1998)
	and

	2) THE MECHANISM OF TELOMERE SIZE CONTROL
	RECENT REFERENCES
	 Li et al. A novel mechanism for telomere size control in
Saccharomyces cerevisiae. Genes Dev. 10: 1310-1326 (1996);
	Lustig, A. The identification of telomerase subunits:  catalysing
telomere research. 7:299-302 (1997);
	Polotnianka et al.  The yeast Ku heterodimer is essential for
protection of the telomere against nucleolytic and recombinational
activities. Current Biology 831-834 (1998)

	Candidates should have a background in molecular biology and/or
genetics.  Background in yeast is preferable but not required. Applicants
must be highly motivated "self-starters".

	Interested candidates should send their c.v. either by e-mail, fax,
or mail to the address listed below.

Arthur J. Lustig
Associate Professor
Department of Biochemistry SL43
Tulane University Medical Center
1430 Tulane Avenue
New Orleans, LA 70112
phone: 504-584-3688
fax: 504-584-2739



From owner-chromosomes@net.bio.net Sat Dec 05 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!news-xfer.newsread.com!netaxs.com!newsread.com!news-nb.rutgers.edu!not-for-mail
From: Mike Cooley <cooley@waksman.rutgers.edu>
Newsgroups: bionet.genome.chromosomes
Subject: CAPS markers won't show heterozygotes
Date: Sun, 06 Dec 1998 00:36:09 -0500
Organization: Rutgers University
Lines: 20
Sender: coooley@coltrane-b-asy-16.rutgers.edu
Message-ID: <366A17C9.45BD4633@waksman.rutgers.edu>
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Hi, I am doing map-based cloning in Arabidopsis and twice now I have
developed CAPS markers only to find that they will not distinguish the
heterozygotes from one of the parents. Assuming that maybe my "known"
heterozygous individual were not really heterozygous I mixed DNA's from
the two parents to "create" a heterozygous DNA and still it came up
homozygous for one of the parents (it is always the same parent). I do
not understand how this occurs. Any ideas?

--
Dr. Michael Cooley
Waksman Institute
Rutgers University
190 Frelinghuysen Rd
Piscataway, NJ 08854

cooley@mbcl.rutgers.edu
phone 732-445-3802
FAX 732-445-5735



From owner-chromosomes@net.bio.net Thu Dec 10 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!newsfeed.wirehub.nl!news.belnet.be!news.rediris.es!news.uva.es!not-for-mail
From: "Rosa Mª Corrales Herrán" <corrales@ioba.med.uva.es>
Newsgroups: bionet.genome.chromosomes
Subject: Northern blot
Date: Fri, 11 Dec 1998 09:41:37 +0100
Organization: Universidad de Valladolid - Spain
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¿Alguien sabe como marcar las sondas de cDNA a utilizar para la
hibridación con el RNA de la membrana que no sea con isotopos
radiactivos?

From owner-chromosomes@net.bio.net Thu Dec 10 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!newsfeed.tli.de!unlisys!news.snafu.de!newscore.ipf.de!newsfeed.metronet.de!RRZ.Uni-Koeln.DE!uni-duisburg.de!news.uni-essen.de!not-for-mail
From: Thomas Radtke <thomas.radtke@uni-essen.de>
Newsgroups: bionet.genome.chromosomes
Subject: human chromosome 11 question
Date: Fri, 11 Dec 1998 08:07:33 +0100
Organization: University Essen, Germany
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Hi,

    Does anyone have a recent information about what percentage
chromosome 11 of the human genom is ?

Thank you very much in advance.

Best regards,

Thomas Radtke



From owner-chromosomes@net.bio.net Thu Dec 10 22:00:00 1998
Path: biosci!SLIP.NET!grizzly
From: grizzly@SLIP.NET (Michael Sherrell)
Newsgroups: bionet.genome.chromosomes
Subject: ACT 396 for sale
Date: 10 Dec 1998 20:19:12 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 14
Sender: daemon@net.bio.net
Distribution: world
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For sale: 1998 Advanced Chemtech Model 396 Multiple Biomolecular Synthesizer, unused, ~2/3 of new price.

Also available:
Perkin Elmer Sciex API III+ LC/MS/MS, electrospray, APCI, $79,000
Hewlett Packard 5989B LC/MS, electrospray, extend mass range, 1995 model, $45,000

Plus many ABI and other sequencers and synthesizers (and service arrangements for synthesizers), flow cytometers, NMRs and SEMs listed on my website.

Michael Sherrell
Grizzly Analytical
707 887 2919/fax 707 887 9834
www.grizzlyanalytical.com

From owner-chromosomes@net.bio.net Sun Dec 13 22:00:00 1998
Path: biosci!MINDSPRING.COM!richandsusan
From: richandsusan@MINDSPRING.COM (Rich and Susan Scheid)
Newsgroups: bionet.genome.chromosomes
Subject: Deciphering Cytogenetics Reports
Date: 13 Dec 1998 21:47:19 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 10
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Distribution: world
Message-ID: <3.0.1.32.19981214004358.0068f380@pop.mindspring.com>
NNTP-Posting-Host: net.bio.net

Hello.  We just had a baby girl who was shown to have a chromosome
abnormality.  We are having a hard time deciphering the report we were
given and wondered if anyone could help us figure it out or give us some
information as to a site that would help us determine what the report
means.  Her karyotype is 46,XX,i(21)(q10).ish 22q11.2(TUPLE1x2) de novo.
We know that she has an isochromosome #21 in place of a normal chromosome.
I thought that any extra genetic material in chromosome #21 signified Down
Syndrome, but our pediatrician said she didn't think that was the case.
Can anyone help?   


From owner-chromosomes@net.bio.net Sun Dec 13 22:00:00 1998
Path: biosci!SLIP.NET!grizzly
From: grizzly@SLIP.NET (Michael Sherrell)
Newsgroups: bionet.genome.chromosomes
Subject: ABI Procise 492 for sale
Date: 14 Dec 1998 15:47:30 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01BE2778.7F0A68E0@oak-pm1-58-186.dialup.slip.net>
NNTP-Posting-Host: net.bio.net



ABI 492 Procise protein sequencer, was $132,000 new, not used in 2 years, $59,000

For sale: 1998 Advanced Chemtech Model 396 Multiple Biomolecular Synthesizer, unused, ~2/3 of new price.

Please call or email if you'd like more details.

Also available: many ABI and other sequencers and synthesizers (and service arrangements for synthesizers), HPLC/MSs, flow cytometers, NMRs and SEMs listed on my website.

Michael Sherrell
Grizzly Analytical
707 887 2919/fax 707 887 9834
www.grizzlyanalytical.com


From owner-chromosomes@net.bio.net Mon Dec 14 22:00:00 1998
Path: biosci!news.stanford.edu!logbridge.uoregon.edu!news.maxwell.syr.edu!newsfeed.cwix.com!203.176.28.184!news.iphil.net!not-for-mail
From: "Eloisa H. Memije" <cherubs@pworld.net.ph>
Newsgroups: bionet.genome.chromosomes
Subject: dog chromosomes
Date: Tue, 15 Dec 1998 15:48:01 +0800
Organization: IPhil Communications Network.  Complaints to <abuse@iphil.net>
Lines: 4
Message-ID: <36761430.69EFB919@pworld.net.ph>
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could anyone help me find a good website which features dog metaphase
chromosomes with some pictures?
MM


From owner-chromosomes@net.bio.net Tue Dec 15 22:00:00 1998
Path: biosci!news.stanford.edu!nntp.stanford.edu!not-for-mail
From: julietteFaraco <faraco@cmgm.stanford.edu>
Newsgroups: bionet.genome.chromosomes
Subject: Re: dog chromosomes
Date: Wed, 16 Dec 1998 12:52:54 +0000
Organization: Stanford University
Lines: 18
Message-ID: <3677AC55.4ADD@cmgm.stanford.edu>
References: <36761430.69EFB919@pworld.net.ph>
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To: cherubs@pworld.net.ph

Cytogenetics of dogs is a bit tricky since the karyotype is really
unforgiving. 38 autosomes plus X and Y, and all but the X are
acrocentric. The sizes are a gradient going from rather small to very
small. 

Here are two URLs for dog genome sites, which may or may not have photos
of dog FISH expts currently.
http://www.fhcrc.org/science/dog_genome/dog.html
http://mendel.berkeley.edu/dog.html

Two references with good karyotype photos are:
1996: chromosome research vol4:306-309
1996 cytogenetics and cell genetics:73: 140-144

I've done a ton of FISH on canine metaphases, but haven't published any
of it. If you have any other questions, I'd be happy to help.
Juliette
Faraco

From owner-chromosomes@net.bio.net Wed Dec 16 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!news.maxwell.syr.edu!diablo.theplanet.net!join.news.pipex.net!pipex!krypton.inbe.net!INbe.net!not-for-mail
From: "ruddy.ro" <ruddy.ro@infonie.be>
Newsgroups: bionet.genome.chromosomes
Subject: DNA content and homogeneity
Date: Thu, 17 Dec 1998 10:58:58 +0100
Organization: Ye 'Ol Disorganized NNTPCache groupie
Lines: 21
Message-ID: <913893801.388775@eole>
NNTP-Posting-Host: eole.infonie.be
X-Newsreader: Microsoft Outlook Express 4.72.3110.5
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Cache-Post-Path: eole!unknown@ppp-charleroi-4-191.infonie.be

Is really DNA exctly the same in an entire organism (comparing what is
comparable : DNA of the same kind of organelles) ?  It seems that at least
for mitochondria, it can not be the case!

What about plastid DNA ? For example, cpDNA content some repeats. So, is it
really so stably organized that what people claims ?
What about DNA of proplastids, leucoplasts, amyloplasts, chloroplasts ...?
Is it the same ? Does it change along plastids differentiation/rediff.
?........

What about even nuclear DNA, especially for a very large and very aged
individual
(giant Sequoia for example): is it exactly the same in every cell,
considering young (or "old juvenile") as well as old trunk cells (even dead
if could still content some DNA) from the tip of the deepest rootlet to the
shoot apex ?






From owner-chromosomes@net.bio.net Thu Dec 17 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!news-stock.gip.net!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!rill.news.pipex.net!pipex!ams.news.uu.net!uunet!in5.uu.net!stalker.oem.net!not-for-mail
From: "Sean" <biostorm@biostorm.com>
Newsgroups: bionet.genome.chromosomes
Subject: karyotyping
Date: Tue, 15 Dec 1998 05:08:41 -0500
Organization: oemnetworks.com
Lines: 27
Message-ID: <36758929.0@rboc1.prime-x.net>
NNTP-Posting-Host: rboc1.prime-x.net
X-Newsreader: Microsoft Outlook Express 4.72.2106.4
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Thought this might be of interest-- my name is Sean Hughes and Im with
Biostorm.  Just making people aware we exist -hope not to offend anyone with
this shamelss plug.:)   But here lies pertinent and economical information.


Chemi-16 Gel documentation & Chemiluminescence:
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Color-Monochrome, (FISH) Automatic Karyotyping  of Chromosomes & Cytogenetic
Analysis
The program performs automatic chromosome analysis of acquired color or
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formats, etc.   $8,500.00

Software 18 Windows, 14 Mac, 24 DOS.
1D main program,           1D  Advanced      2D advanced
DNA sequence reading,  PCR, RFLP,          RAPD DNA fingerprinting
Dots-ELISA        Image analysis     Color/B&W image analysis Colony
count-sort             Particle size analysis      Similarity dendrograms
----------------------------------------------------------------------------
-----------------------------------------



From owner-chromosomes@net.bio.net Fri Dec 18 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!newsfeed.cwix.com!206.172.150.11!news1.bellglobal.com!news.agtac.net!newsfeed.telusplanet.net!news2.telusplanet.net.POSTED!not-for-mail
Message-ID: <367B1790.AF175539@telusplanet.net>
From: Lou Frankow <amazing@telusplanet.net>
Organization: http://www.telusplanet.net/public/amazing/lou.html
X-Mailer: Mozilla 4.5 [en] (Win98; I)
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Newsgroups: bionet.genome.chromosomes
Subject: Help
Content-Type: multipart/mixed;
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This is a multi-part message in MIME format.
--------------29ED5B03AAE940012AEB5EB4
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I am looking for information on an alleged chromosome deficient
condition, what we are told is that 8P+ is missing, anybody ever hear
about this?? Haven't found anyone in Canada.Lou

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begin:vcard 
n:Frankow;Lou
tel;fax:403-529-2746
tel;work:403-502-0293
x-mozilla-html:FALSE
url:http://www.telusplanet.net/public/amazing/lou.html
org:Lou's Plumbing/Gasfitting
adr:;;1202 Ross St. S.E.;Medicine Hat;Alberta;T1A-0Y6;CANADA
version:2.1
email;internet:amazing@telusplanet.net
fn:Lou Frankow
end:vcard

--------------29ED5B03AAE940012AEB5EB4--


From owner-chromosomes@net.bio.net Sat Dec 19 22:00:00 1998
Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.gtei.net!newsfeed.wli.net!howland.erols.net!vixen.cso.uiuc.edu!ux10.cso.uiuc.edu!jiezhang
From: jie zhang <jiezhang@students.uiuc.edu>
Newsgroups: bionet.genome.chromosomes
Subject: help!! (sequence editing)
Date: Sun, 20 Dec 1998 10:49:57 -0600
Organization: University of Illinois at Urbana-Champaign
Lines: 23
Message-ID: <Pine.SOL.3.96.981220104815.26802B-100000@ux10.cso.uiuc.edu>
Reply-To: jie zhang <jiezhang@students.uiuc.edu>
NNTP-Posting-Host: ux10.cso.uiuc.edu
Mime-Version: 1.0
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X-Sender: jiezhang@ux10.cso.uiuc.edu

Hi, I am working on a candidate gene for an autoimmune disease. Since I am
new in this field, one question always bothers me during sequence editing.
To compare the polymorphism of the gene among different strains of mice,
we usually sequence 3 or more colonies of one segment at about 700bp (DNA
or mRNA) in one strain. If 5 or more sequences agree to one nucleotide
(for example 'A') at one position in one strain of mouse,  only one
sequence shows different nucleotide ('T', for example, but there is a
signal of 'A' right below the 'T') at this position.  My question is if I
have to change the one sequence to agree other 5 sequences including it's
complement sequence, or just leave it intact to keep the original data?

Appreciate any suggestion!!              


Jay (Jie) Zhang
Vet. Pathobiology
University of Illinois
2001 S. Lincoln
Urbana, IL 61801
217-244-9540(O)
217-244-7421(Fax)



From owner-chromosomes@net.bio.net Sat Dec 19 22:00:00 1998
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.cwix.com!194.162.162.196!newsfeed.nacamar.de!rill.news.pipex.net!pipex!ams.news.uu.net!uunet!in3.uu.net!satlink!ul3.satlink.com!not-for-mail
From: File Detect <File@Detect.com>
Newsgroups: bionet.genome.chromosomes
Subject: Useful program
Date: Sun, 20 Dec 1998 00:20:11 -0600
Organization: Marsc
Lines: 71
Message-ID: <367C971B.1FDB83@Detect.com>
NNTP-Posting-Host: maq033b.advance.com.ar
Mime-Version: 1.0
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X-Mailer: Mozilla 4.03 [en] (Win95; I)


--------------A969C1E6A26D03759D4CCB83
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit

File Detect:

File Detect is a Windows 95 program.
This program detects new, modified or deleted files in your disk.
Also detects new or deleted folders (Directories).
This is very useful to know what files (As to be *.DLL's, *.OCX's,
*.TTF's
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driver or make a change in the system configuration.

As you know, when you install a program or driver in your disk, a lot of

files are installed in your system folders, Some times you uninstall a
program
and these files remain there occupying several MB of space in your disk.

Think about it. How many times do you download a program from the
Internet?.
How many games?.
With File Detect you can take control on this situation since all what
is
copied to your disk is detected.

For more information about this program and for downloading it visit
the home page: www.angelfire.com/fl/filedetect



--------------A969C1E6A26D03759D4CCB83
Content-Type: text/html; charset=us-ascii
Content-Transfer-Encoding: 7bit

<HTML>
<B><FONT SIZE=+1>File Detect:</FONT></B>

<P>File Detect is a Windows 95 program.
<BR>This program detects new, modified or deleted files in your disk.
<BR>Also detects new or deleted folders (Directories).
<BR>This is very useful to know what files (As to be *.DLL's, *.OCX's,
*.TTF's
<BR>*.HLP's and many other) are copied to your disk when you install a
new program,
<BR>driver or make a change in the system configuration.

<P>As you know, when you install a program or driver in your disk, a lot
of
<BR>files are installed in your system folders, Some times you uninstall
a program
<BR>and these files remain there occupying several MB of space in your
disk.

<P>Think about it. How many times do you download a program from the Internet?.
<BR>How many games?.
<BR>With File Detect you can take control on this situation since all what
is
<BR>copied to your disk is detected.

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<BR>the <A HREF="http://www.angelfire.com/fl/filedetect">home page: www.angelfire.com/fl/filedetect</A>
<BR>&nbsp;
<BR>&nbsp;</HTML>

--------------A969C1E6A26D03759D4CCB83--



From owner-chromosomes@net.bio.net Mon Dec 21 22:00:00 1998
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey03.news.aol.com!not-for-mail
From: rcjohnsen@aol.com (Rcjohnsen)
Newsgroups: bionet.genome.chromosomes
Subject: Re: Deciphering Cytogenetics Reports
Lines: 6
NNTP-Posting-Host: ladder03.news.aol.com
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Date: 22 Dec 1998 22:10:12 GMT
Organization: AOL http://www.aol.com
References: <3.0.1.32.19981214004358.0068f380@pop.mindspring.com>
Message-ID: <19981222171012.21603.00000208@ng-fp1.aol.com>

Your pediatrician is right.  To find out more about this go to the OMIM site on
Human Genetics.
It address is  http://www.ncbi.nlm.nih.gov/Omim/
Scroll down the page until you reach search database and click on it.  A new
page (search engine) asks for key words.  Type in Down Syndrome.  The heading
of interest to you is keyregion

From owner-chromosomes@net.bio.net Mon Dec 21 22:00:00 1998
Path: biosci!agate!newsfeed.berkeley.edu!news.maxwell.syr.edu!news-ge.switch.ch!news.rediris.es!news.uam.es!not-for-mail
From: "jose carlos garcia garcia" <jcarlos.garcia@adi.uam.es>
Newsgroups: bionet.genome.chromosomes
Subject: saludo navideño
Date: 22 Dec 1998 14:11:35 GMT
Organization: UAM
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Feliz navidad a todos.
Es la primera vez que conecto, y me alegrará mantener conversaciones o
discusiones con todos los que esteis por ahí. Me alegraría que mandarais
alguna contestación, solo para saber si hay alguien.
Feliz navidad para todos.

From owner-chromosomes@net.bio.net Tue Dec 22 22:00:00 1998
Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.gtei.net!europa.clark.net!195.173.173.151!woodstock.news.demon.net!demon!news.demon.co.uk!demon!pharm-tech.demon.co.uk!not-for-mail
From: support@pharm-tech.demon.co.uk
Newsgroups: bionet.genome.chromosomes
Subject: New Magnetic Bead Technology
Date: Wed, 23 Dec 1998 15:29:19 GMT
Organization: PHARMACEUTICAL technology Ltd
Message-ID: <54931G50.DV67J6FK@pharm-tech.demon.co.uk>
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This is a multi-part message in MIME format.

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Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit

PHARMACEUTICAL technology Ltd has recently launched  a new magnetic
bead separation technology for semi-automated and automated methods.

Please visit our web site for further details.

John Bates Ph.D


--=_9yn00s0337
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit

PHARMACEUTICAL 
technology Ltd

32 Regal Road
Wisbech
Cambs PE13 2RQ
United Kingdom

Tel  (+44) 01945 476 716
Fax  (+44) 01945 476 718
support@pharm-tech.demon.co.uk
http://www.pharm-tech.demon.co.uk

--=_9yn00s0337--


From owner-chromosomes@net.bio.net Sat Dec 26 22:00:00 1998
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.genome.chromosomes
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 27 Dec 1998 02:00:14 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199812271000.CAA18897@net.bio.net>
NNTP-Posting-Host: net.bio.net

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


