From owner-diagnostics@net.bio.net Sat Apr 01 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!agate!howland.reston.ans.net!pipex!peernews.demon.co.uk!demon!firthcom From: Steve Firth Subject: Serum samples wanted X-Nntp-Posting-Host: firthcom.demon.co.uk Message-ID: <95033113572500193@firthcom.demon.co.uk> Sender: news@demon.co.uk (Usenet Administration) Reply-To: steve@firthcom.demon.co.uk Organization: Firth Consulting Date: Fri, 31 Mar 1995 13:57:25 GMT X-Mailer: rnMac v1.3b5 Lines: 11 I need samples from patients receiving HRT for a research project. The samples must be fresh and collected from a UK site. If anyone is able to help, please contact me via email, and I will send an outline of the collection protocol. ....................................................................... Stephen Firth Matthew: 27, 5 steve@firthcom.demon.co.uk Judges: 7, 17 CIS: 100023,3414 From owner-diagnostics@net.bio.net Sat Apr 01 23:00:00 1995 Path: biosci!RT66.COM!ingen From: ingen@RT66.COM Newsgroups: bionet.diagnostics Subject: (none) Date: 2 Apr 1995 08:06:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504021507.AA26234@RT66.com> NNTP-Posting-Host: net.bio.net Subscribe In C. Kim From owner-diagnostics@net.bio.net Sun Apr 02 23:00:00 1995 Path: biosci!PARGVA.AGR.CA!DEBOER From: DEBOER@PARGVA.AGR.CA Newsgroups: bionet.diagnostics Subject: PCR-related techniques for detecting food born pathogens. Date: 3 Apr 1995 15:55:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HOWV77I5Z60007RI@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Dear netters, A friend of mine is thinking of a project on application of PCR- related techniques for detecting food borne pathogens in milk and eggs. Despite the possible PCR-inhibitory substances in the DNA extracts from raw samples, DNA from dead cells may give false positive results. How can we solve this problem? Is there any related references? Any suggestion will be appreciated. Xiang Li Agriculture Canada Vancouver Research Station. From owner-diagnostics@net.bio.net Sun Apr 02 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!pipex!news.pavilion.co.uk!line03.kemp-du.pavilion.co.uk!user From: jpwscyto@pavilion.co.uk (Jon Waterman-Smith) Newsgroups: bionet.chlamydomonas,bionet.diagnostics,bionet.drosophila,bionet.general,bionet.genome.arabidopsis,bionet.genome.chrom22,bionet.genome.chromosomes Subject: LAUNCH OF FREE MAGS/WWW PAGES FOR BIONET... Date: 3 Apr 1995 09:48:11 GMT Organization: Pavilion Internet plc, Brighton, England Lines: 29 Message-ID: NNTP-Posting-Host: line03.kemp-du.pavilion.co.uk Xref: biosci bionet.chlamydomonas:502 bionet.diagnostics:55 bionet.drosophila:978 bionet.general:14454 bionet.genome.arabidopsis:3163 bionet.genome.chromosomes:529 Within the next 6 weeks a new series of magazines will be launched, which will have a hard copy available to interested parties, as well as a major publication series available on the web. The magazines will deal with many subject areas, and will have hypertext points imprinted into features, so the reader can access the WWW, and simply go to the relevant pages, following the same hypertext links as shown in the magazines. The future....... Our aim is to promote science, and scientific issues mainly in the field of bioscience. Both the magazines and the WWW pages will eventually have articles and links to publications, posters, and as many products and suppliers as we can get interested in publishing with us. So, if you know of anyone who would be interested in providing articles, publishing information to assist other scientists, or you work for a company who wants to advertise , and set up pages on the WWW, we aim to become the one stop point to leap off into the web, and other internet areas. Send me an email, and I'll keep you up to date on our progress. Also let me know if you want to register for the first issue of the first magazine to be published early May '95. Diagnostics, Biotechnology and Life-sciences will be the areas first targeted for publication. TO ALL INTERESTED ADVERTISERS - the deadline for the first issue will be in 3-4 weeks time. Please contact me urgently for more information - your advert will also secure you web space and hypertext links!!!!! I look forward to hearing from you all... Best wishes, Jon Waterman-Smith E-mail address..... jpwscyto@pavilion.co.uk From owner-diagnostics@net.bio.net Sun Apr 02 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!pipex!news.pavilion.co.uk!line03.kemp-du.pavilion.co.uk!user From: jpwscyto@pavilion.co.uk (Jon Waterman-Smith) Newsgroups: bionet.diagnostics Subject: LAUNCH OF FREE MAGS/WWW PAGES FOR BIONET... Date: 3 Apr 1995 09:30:47 GMT Organization: Pavilion Internet plc, Brighton, England Lines: 29 Message-ID: NNTP-Posting-Host: line03.kemp-du.pavilion.co.uk Within the next 6 weeks a new series of magazines will be launched, which will have a hard copy available to interested parties, as well as a major publication series available on the web. The magazines will deal with many subject areas, and will have hypertext points imprinted into features, so the reader can access the WWW, and simply go to the relevant pages, following the same hypertext links as shown in the magazines. The future....... Our aim is to promote science, and scientific issues mainly in the field of bioscience. Both the magazines and the WWW pages will eventually have articles and links to publications, posters, and as many products and suppliers as we can get interested in publishing with us. So, if you know of anyone who would be interested in providing articles, publishing information to assist other scientists, or you work for a company who wants to advertise , and set up pages on the WWW, we aim to become the one stop point to leap off into the web, and other internet areas. Send me an email, and I'll keep you up to date on our progress. Also let me know if you want to register for the first issue of the first magazine to be published early May '95. Diagnostics, Biotechnology and Life-sciences will be the areas first targeted for publication. TO ALL INTERESTED ADVERTISERS - the deadline for the first issue will be in 3-4 weeks time. Please contact me urgently for more information - your advert will also secure you web space and hypertext links!!!!! I look forward to hearing from you all... Best wishes, Jon Waterman-Smith E-mail address..... jpwscyto@pavilion.co.uk From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!NETCOM.COM!gothmog From: gothmog@NETCOM.COM (En ring til aa herske) Newsgroups: bionet.diagnostics Subject: unsubscribe diagnost Date: 4 Apr 1995 08:21:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net unsubscribe diagnost From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!MEDUSA.UNM.EDU!BHJELLE From: BHJELLE@MEDUSA.UNM.EDU Newsgroups: bionet.diagnostics Subject: (none) Date: 4 Apr 1995 14:43:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HOY2WENM4K001R03@MEDUSA.UNM.EDU> NNTP-Posting-Host: net.bio.net unsubscribe From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.diagnostics Subject: sample collection Date: 4 Apr 1995 14:36:13 -0700 Organization: University of Arkansas Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <1FA3363D96@mercury.uark.edu> I would like to poll the this newsgroup as to what methods others might recommend for sample collection. We need to collect microliter amounts of blood for PCR analysis. Unfortunately, the samples should be most easily collected by untrained animal handlers. We have looked into cap tubes and the micro-vacutainer type collection tubes but all of these require using a lancet and then collection from skin surfaces. It would be really nice if there was a vacutainer/needle system that collects only 10-100 microliters but those systems start at around 2 ml. Since we are using PCR we are very concerned that with surface collection there can be cross-contamination from handlers hands and from dander on the skin surface. We also don't want to have to design something. Does anyone have any suggestions where we might look?? //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!STRAUSS.UDEL.EDU!gell From: gell@STRAUSS.UDEL.EDU (Scott Denni Gellman) Newsgroups: bionet.diagnostics Subject: (none) Date: 4 Apr 1995 10:19:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe diagnost From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!biotech.lan.nrc.ca!juck From: juck@biotech.lan.nrc.ca ("Juck, David") Newsgroups: bionet.diagnostics Subject: Re: PCR-related techniques for detecting Date: 4 Apr 1995 08:47:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <2F816A14@coursmtp.nrc.ca> NNTP-Posting-Host: net.bio.net >How about devising a system based on detecting RNA? This is degraded >faster than DNA, so you'd have less chance of false positives based on >nucleic acids from dead cells. >Another alternative approach would be to follow up any PCR positives >with traditional plating-out, with a positive result in both required for >confirmation of the presence of the pathogen in food. There would still be >an advantage in using PCR because it would screen out the negative >samples. >No references for either of these - just a couple of ideas that occured to >me over my mid-morning cuppa:-) >Kevin O'Donnell >Scottish Agricultural Science Agency >Edinburgh >Scotland With respect to detecting RNA using PCR, there is a commercially available kit for RT-PCR (reverse transcriptase-PCR) produced by Perkin-Elmer which first produces a DNA template from the RNA in the sample, and then normal PCR is performed on the DNA template. This should be a good indicator of metabolic activity of the cell, but if the cell is in a dormant state, false negatives may be a factor. Choice of the target sequence then becomes important. I have not used RT-PCR, but someone in my lab is currently using it in soils and is getting interesting results. With plating out of the samples, the same problem is possible with dormant cells not forming colonies. After an incubation time, cells may become active and start to reproduce. I don't mean to play the devil's advocate, this may not be a problem on foods, but in water monitoring, it can be an important factor. David Juck Biotechnology Research Institute Montreal, Canada. From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Re: PCR-related techniques for detecting food born pathogens. Organization: Scottish Agricultural Science Agency Date: Tue, 4 Apr 1995 09:56:34 GMT Message-ID: X-Newsreader: WinVN 0.91.6 References: <01HOWV77I5Z60007RI@GW.AGR.CA> Sender: news@jura.sasa.gov.uk (Usenet) Lines: 17 How about devising a system based on detecting RNA? This is degraded faster than DNA, so you'd have less chance of false positives based on nucleic acids from dead cells. Another alternative approach would be to follow up any PCR positives with traditional plating-out, with a positive result in both required for confirmation of the presence of the pathogen in food. There would still be an advantage in using PCR because it would screen out the negative samples. No references for either of these - just a couple of ideas that occured to me over my mid-morning cuppa:-) Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland From owner-diagnostics@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!VTH1.VTH.COLOSTATE.EDU!dgetzy From: dgetzy@VTH1.VTH.COLOSTATE.EDU (David Getzy) Newsgroups: bionet.diagnostics Subject: PCR detection of pathogens of veterinary clinical importance. Date: 4 Apr 1995 06:47:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Fellow diags: We are in the process of setting up a PCR laboratory. Initially, the work that will be done relates to pathogenesis of bovine herpesviral infection and the pathogenesis of toxoplasmal-induced occular damage. I am interested in locating references or obtaining comments on folks that are using this technology currently for the detection of pathogens of veterinary clinical importance- specifically what pathogens have they found this methodology useful and " economically-practical". Also, I am interested if there is a need for specific development of PCR-based testing for certain pathogens that currently diagnostic problems (ie-for what pathogens would a PCR assay be useful for its detection/significance?). From the literature, I am generally aware of some of the more common assays currently performed (pseudorabies/vaccine recombinants, avian circovirus, etc.). Thanks in advance for your input. If sufficient responses are obtained, I would be happy to summarize them for the list. Thanks again. Dave ********************************************************************* * Dave Getzy * Director * CSU Diagnostic Laboratories * 300 West Drake, Fort Collins, Co 80523 * PHONE 303-491-1281 FAX 303-491-0320 * EMAIL dgetzy@vth1.vth.colostate.edu ******************************************************************** From owner-diagnostics@net.bio.net Tue Apr 04 23:00:00 1995 Path: biosci!ACADEM01.MTY.ITESM.MX!dsleal From: dsleal@ACADEM01.MTY.ITESM.MX ("M.C. Diana Sara Leal Klevezas") Newsgroups: bionet.diagnostics Subject: Re: PCR-related techniques for detecting food born pathogens. Date: 5 Apr 1995 08:08:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <01HOWV77I5Z60007RI@GW.AGR.CA> NNTP-Posting-Host: net.bio.net On 3 Apr 1995 DEBOER@pargva.agr.ca wrote: > Dear netters, > > A friend of mine is thinking of a project on application of PCR- > related techniques for detecting food borne pathogens in milk and > eggs. Despite the possible PCR-inhibitory substances in the DNA > extracts from raw samples, DNA from dead cells may give false > positive results. How can we solve this problem? > > Is there any related references? Any suggestion will be > appreciated. > > Xiang Li > > Agriculture Canada > Vancouver Research Station. > > Dr. Griffits, at the Food science department at the U. of Guelph, Ontario, Canada is working with Salmonella-contaminated eggs is a neat way. He constructed a Salmonella phage carrying and expressing a Luciferase gene, which is then injected into the eggs.If they glow,means that Salmonella is present and alive since it allows the expression of the selectable marker. I understand it's working nicely. The U of Guelph's phone number is (595) 8244120 (check the area code, I'm not possitive), the fellow's name is Mansell (or sounds like it) Griffits. For the milk problem we are writting a project about the detection of Brucella on milk samples. We have developed the protocol for the DNA extraction and PCR which is working quite fine. We have wondered if after pasteurization or other kind of processing there will be death bacteria to keep the test appearing possitive. Please, get your friend to contact us to share our ideas with him/her. Best Regards form Sunny Mexico Diana Sara Leal Klevezas Centro de Investigacion Biomedica del Noreste Instituto Mexicano del Seguro Social San Luis Potosi y 2 de abril, Col. Independencia Monterrey, N.L. tel and fax. (52) 344 4116 From owner-diagnostics@net.bio.net Tue Apr 04 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!doc.news.pipex.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Subscribing and unsubscribing Organization: Scottish Agricultural Science Agency Date: Wed, 5 Apr 1995 13:57:16 GMT Message-ID: X-Newsreader: WinVN 0.91.6 Sender: news@jura.sasa.gov.uk (Usenet) Lines: 19 Now that bionet.diagnostics is a full newsgroup, many people with newsreader software will prefer to access the group using this rather than the mailing list. The correct address for this is: biosci-server@net.bio.net and the message you need is : unsubscribe diagnost All this is assuming that you were a member of the original prototype newsgroup. Kevin Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland From owner-diagnostics@net.bio.net Wed Apr 05 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netcomsv!uu3news.netcom.com!www1.chiron.com!cc.chiron.com!Julio_Gagne From: Julio_Gagne@cc.chiron.com (Julio Gagne) Newsgroups: bionet.diagnostics Subject: Kerry Mulllis on OJ trial Date: Wed, 5 Apr 1995 16:01:33 GMT Organization: Chiron Corporation Lines: 12 Message-ID: NNTP-Posting-Host: 165.140.33.5 X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] diagnostics...pcr...Kerry Mullis...OJ! My early prediction is that Kerry will upstage Kato Kaelin on the stand and land himself a movie deal. Maybe he'll get the lead in the remake of Planet of the Apes that Oliver Stone is working on. =============================================================== It is not enough to be busy, so are the ants. -Henry David Thoreau Email Address: Julio_Gagne@cc.chiron.com =============================================================== From owner-diagnostics@net.bio.net Wed Apr 05 23:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!agate!kos5mac6.berkeley.edu!user From: spenson@nature.berkeley.edu (Simon Penson) Newsgroups: bionet.diagnostics,bionet.biophysics Subject: GC-MS analysis of cyclic nucleotides Date: Thu, 06 Apr 1995 13:02:11 -0800 Organization: UC Berkeley; Plant Biology Lines: 17 Message-ID: NNTP-Posting-Host: kos5mac6.berkeley.edu Xref: biosci bionet.diagnostics:67 bionet.biophysics:822 Dear Netters, I'm interested in analysing for cGMP in extracts of plants tissue. I will probably use an RIA kit for more routine work, but I will also need to use a more unequivocal method at some point. GC-MS would be useful. My question is: Has GC-MS been used for cGMP determination, and what are the detection limits for this technique? I can explain more fully if you think that would be useful!! Thanks in advance, Simon. Simon Penson Plant Biology UC Berkeley. Tel: (510) 642 6084 From owner-diagnostics@net.bio.net Sun Apr 09 23:00:00 1995 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!doc.news.pipex.net!pipex!peernews.demon.co.uk!news2.demon.co.uk!serratia From: mike@serrratia.demon.co.uk (Michael Gaston) Newsgroups: bionet.diagnostics Subject: Verotoxin/E.coli O157 Date: 10 Apr 1995 20:11:49 +0100 Organization: Demon Internet (news2) Lines: 15 Sender: news@news2.demon.co.uk Distribution: world Message-ID: <3mbvtl$fh9@distort.demon.co.uk> NNTP-Posting-Host: distort.demon.co.uk X-Nntp-Posting-Host: serratia.demon.co.uk X-Newsreader: News Xpress Version 1.0 Beta #2 It seems likely that the current rapid latex tests for O157 antigen don't help in the diagnosis of verotoxin producing non-O157 E.coli. I work for a diagnostic company and we are thinking about developing an immunoassay for verotoxins. In the first instance this would probably be a culture-confirmation test rather than a direct faecal screen. However, its not clear to me whether a test for verotoxin would be of sufficient diagnostic value to be useful in a clinical laboratory. If anyone has experience of using such a test, home-brewed or otherwise, I'd be greatly interested in their views. Thanks in advance, Mike From owner-diagnostics@net.bio.net Tue Apr 11 23:00:00 1995 Path: biosci!AVA.BCC.ORST.EDU!volke From: volke@AVA.BCC.ORST.EDU ("Elzibeth Volk") Newsgroups: bionet.diagnostics Subject: Quick Dialysis Date: 12 Apr 1995 11:53:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <42915.volke@bcc.orst.edu> NNTP-Posting-Host: net.bio.net Has anyone tried out Pierce's Slide-A-Lyzer 10K dialysis cassettes for dialysis in purification of monoclonal or polyclonal antibodies? ELZI VOLK Small Fruits Certification and Virus Elimination Program OSU Corvallis, OR volke@bcc.orst.edu From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.diagnostics Subject: Re: sample collection Date: 13 Apr 1995 12:06:08 -0700 Organization: University of Arkansas Lines: 60 Sender: daemon@net.bio.net Distribution: world Message-ID: <6493FA5C66@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > To: diagnost@net.bio.net > From: bwallace@genetics.bio-rad.com (Bruce Wallace) > Subject: Re: sample collection > Date: 13 Apr 1995 08:36:35 -0700 > >In article <1FA3363D96@mercury.uark.edu>, DRHOADS@MERCURY.UARK.EDU > >("Douglas Rhoads") says: > >> > >>I would like to poll the this newsgroup as to what methods others > >>might recommend for sample collection. We need to collect microliter > >>amounts of blood for PCR analysis. Unfortunately, the samples should > >>be most easily collected by untrained animal handlers. We have > >>looked into cap tubes and the micro-vacutainer type collection tubes > >>but all of these require using a lancet and then collection from skin > >>surfaces. It would be really nice if there was a vacutainer/needle > >>system that collects only 10-100 microliters but those systems start > >>at around 2 ml. Since we are using PCR we are very concerned that > >>with surface collection there can be cross-contamination from > >>handlers hands and from dander on the skin surface. We also don't > >>want to have to design something. > >> > >>Does anyone have any suggestions where we might look?? > >> > > Doug, > > Blood is routinely collected from infants by puncturing the heel with a > lancet, blotting the blood onto filter paper (S&S 903) and drying the > specimen. This method of collecting blood has been shown to be adequate > for use in DNA probe analysis including PCR and LCR. More importantly, > there does not seem to be a problem of cross contamination when filter > papers containing dried blood spots from different individuals are stored > in physical contact with each other. > > Bruce Wallace > I would agree with everything you say. However, in my case the samples will not be collected by a trained health care provider. I have looked into the microlancets and considered capillary tubes. I have seen how this works when field workers have been asked to collect. We end up with blood on both ends of the cap tube and all over the shipping container. At that point we don't know where the blood smear comes from and could be contaminations from other animals recently collected. One thing I have considered is a kit of a lancet, cap tube, shipping container, alcohol swab and latex gloves. One set for each sample. I was just hoping someone had built a better mouse (blood) trap. Something like a small lancet that has a hollow point and would collect a few microliters of blood inside the lancet. am trying to limit //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!sunsite.doc.ic.ac.uk!doc.news.pipex.net!pipex!sasa.gov.uk!news From: burns@sasa,gov.uk (Robert Burns) Subject: Re: sample collection Organization: Scottish Agricultural Science Agency Date: Thu, 13 Apr 1995 13:11:51 GMT Message-ID: X-Newsreader: WinVN 0.91.3 References: <1FA3363D96@mercury.uark.edu> Sender: news@jura.sasa.gov.uk (Usenet) Lines: 45 In article <1FA3363D96@mercury.uark.edu>, DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") says: > >I would like to poll the this newsgroup as to what methods others >might recommend for sample collection. We need to collect microliter >amounts of blood for PCR analysis. Unfortunately, the samples should >be most easily collected by untrained animal handlers. We have >looked into cap tubes and the micro-vacutainer type collection tubes >but all of these require using a lancet and then collection from skin >surfaces. It would be really nice if there was a vacutainer/needle >system that collects only 10-100 microliters but those systems start >at around 2 ml. Since we are using PCR we are very concerned that >with surface collection there can be cross-contamination from >handlers hands and from dander on the skin surface. We also don't >want to have to design something. > >Does anyone have any suggestions where we might look?? > >//========================================================\\ >||Doug Rhoads || Dept. of Biological Sciences|| >||drhoads@mercury.uark.edu || 601 Science Engineering || >||drhoads@uafsysb.uark.edu || University of Arkansas || >||501-575-3251 || Fayetteville, AR 72701 || >==========================================================|| >|| My Dogma Just Got Run Over by Someone's Karma || >\\========================================================// Doug, I don't know of any small collection devices that would suit your purpose but here is a little trick that might work for you. Smear some vaseline or other hydrophobic grease at the sample site and do a skin stab with a lancet through it. The blood will collect on the surface of the grease as a little blob which can easily be taken up by capillarity into a capillary tube. There is also a product which surgeons use called opsite which sticks to the skin surface and can be cut or injected through. I don't know if this would give enough freedom of contact from the skin but might work. Regards Robert From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!NFRSSJ.AGR.CA!HAMPSONM From: HAMPSONM@NFRSSJ.AGR.CA Newsgroups: bionet.diagnostics Subject: address change Date: 13 Apr 1995 04:51:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HPA6VA07XU00184U@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Please note that as of this message, my address is now hampsonm@nfrssj.agr.ca. HQ, please note, else you can't contact me. Mike Hampson. From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: bourgaud@ensaia.u-nancy.fr (Frederic Bourgaud) Newsgroups: bionet.diagnostics Subject: Morphine and ELISA Date: 13 Apr 1995 09:26:51 +0100 Lines: 21 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3min8b$4o5@mserv1.dl.ac.uk> Original-To: diagnost@dl.ac.uk Hi all, I am starting a new research program dealing with morphine in plants. I am looking for a rapid/reliable ELISA test that would be adapted to this. I already know that ELISA tech.(Lexington, Kentucky) commercialises a kit called "generic opiate ELISA test". It's a competition test and involves polyclonal antobodies. It is not very specific to morphine as it crosses with all the related alkaloids. It costs about 90 US $ per plate. Does anybody know another ELISA test, maybe based on monoclonal antibodies ? It is important to me to have a test more specific to morphine. Also any experience/reference on morphine quantification in plants with ELISA would be appreciated. Also posted to the Biotech and Plant-TC discussion lists. Sorry for multiple postings. Thanks Fred (bourgaud@ensaia.u-nancy.fr) From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!GENETICS.BIO-RAD.COM!bwallace From: bwallace@GENETICS.BIO-RAD.COM (Bruce Wallace) Newsgroups: bionet.diagnostics Subject: Re: sample collection Date: 13 Apr 1995 08:36:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >In article <1FA3363D96@mercury.uark.edu>, DRHOADS@MERCURY.UARK.EDU >("Douglas Rhoads") says: >> >>I would like to poll the this newsgroup as to what methods others >>might recommend for sample collection. We need to collect microliter >>amounts of blood for PCR analysis. Unfortunately, the samples should >>be most easily collected by untrained animal handlers. We have >>looked into cap tubes and the micro-vacutainer type collection tubes >>but all of these require using a lancet and then collection from skin >>surfaces. It would be really nice if there was a vacutainer/needle >>system that collects only 10-100 microliters but those systems start >>at around 2 ml. Since we are using PCR we are very concerned that >>with surface collection there can be cross-contamination from >>handlers hands and from dander on the skin surface. We also don't >>want to have to design something. >> >>Does anyone have any suggestions where we might look?? >> Doug, Blood is routinely collected from infants by puncturing the heel with a lancet, blotting the blood onto filter paper (S&S 903) and drying the specimen. This method of collecting blood has been shown to be adequate for use in DNA probe analysis including PCR and LCR. More importantly, there does not seem to be a problem of cross contamination when filter papers containing dried blood spots from different individuals are stored in physical contact with each other. Bruce Wallace --/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/-- Bruce Wallace, Molecular Diagnostics Tel: 510-741-6532 Bio-Rad Laboratories Fax: 510-741-1051 2000 Alfred Nobel Dr. Hercules CA 94547 email: bwallace@genetics.bio-rad.com From owner-diagnostics@net.bio.net Wed Apr 12 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: blood collection devices for PCR Date: 13 Apr 1995 06:49:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, Doug Rhodes enquired about collection devices for small quantities of blood for use in PCR. I don't know of any small volume vacutainer type devices but this little trick might help. Before taking sample place a layer of vaseline or some other hydrophobic grease on the skin at the sample site. punture the skin through the grease and a small bead of blood will be produced. This should have had no contact with the skin due to the grease and can be easily removed using a capillary tube (approx 200ul). Don't know if this will work but it is cheap and easy and might be worth a try. BTW there is also a plastic film material called opsite which surgeons use to prevent skin contamination of wounds. Apply to skin and either cut or inject through it. Hope this helps Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Thu Apr 13 23:00:00 1995 Path: biosci!IX.NETCOM.COM!mpatnaik From: mpatnaik@IX.NETCOM.COM (M Patnaik) Newsgroups: bionet.diagnostics Subject: Toxoplasma vaccine/Collaboration Date: 13 Apr 1995 19:34:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504140234.TAA01121@ix5.ix.netcom.com> NNTP-Posting-Host: net.bio.net We are a R&D driven reference laboratory in Santa Monica and have several projects in the pipeline . I am interested in finding out if anyone is currently working on or is aware of an animal vaccine for toxoplasma gondii. I am also looking for possible collaborators to study the role this parasite plays in both immunocompromised as well as immunocompetent individuals worldwide . I have recently obtained some very interesting data regarding this and am interested in shring this and continuing thi study with somebody who has access to bonafide samples. I would appreciate any information or addresses. Sincerely, Meeta Patnaik, M.D. Research Scientist Specialty Laboratories Inc. From owner-diagnostics@net.bio.net Thu Apr 13 23:00:00 1995 Path: biosci!agate!msunews!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!torn!news.unb.ca!nbt.nbnet.nb.ca!jasond.nbnet.nb.ca!jasond From: jasond@nbnet.nd.ca (Jason Duplessis) Newsgroups: bionet.diagnostics Subject: $$$ F A S T CASH $$$ Date: Thu, 13 Apr 1995 07:00:49 Organization: Province of New Brunswick Lines: 131 Message-ID: NNTP-Posting-Host: jasond.nbnet.nb.ca X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] SUBJECT: Get ALL THE MONEY YOU WANT!No Work! PLEASE LISTEN BEFORE YOU START FLAMING ME AND GET IGNORED SEVERLY. 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The following letter was written by a participating member in this program. *********************************************************************** To those with the COMMON sense to participate in this easy money opportunity: About six months ago I received the enclosed post in letter form. I ignored it. I received about five more of the same letter within the next two week. I ignored them also. Of course, I was tempted to follow through and dreamed of making thousands, but I was convinced it was just another gimmick and could not possibly work. I was wrong! About three weeks later I saw this same letter posted on a local bulletin board in Montreal. I liked the idea of giving it a try with my computer. I didn't expect much because I figured, if other people were as skeptical as I, they would not be too quick to part with $5.00. But, I BUY LOTTERY TICKETS WEEKLY IN MY PROVINCE AND HAVE NOTHING TOO SHOW FOR IT BUT TICKET STUBS! 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Good Luck, Charles Kust Good Luck, Anne From owner-diagnostics@net.bio.net Fri Apr 14 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!news.Stanford.EDU!unixhub!fnnews.fnal.gov!nntp-server.caltech.edu!netline-fddi.jpl.nasa.gov!elroy.jpl.nasa.gov!news.msfc.nasa.gov!bcm!cs.utexas.edu!howland.reston.ans.net!usc!crash!kiehl From: b3748@cts.com (Bryan Kiehl) Subject: Re: Verotoxin/E.coli O157 Organization: CTSNET Date: Wed, 12 Apr 1995 19:10:01 GMT Message-ID: X-Newsreader: News Xpress Version 1.0 Beta #2 References: <3mbvtl$fh9@distort.demon.co.uk> Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: kiehl.cts.com Lines: 18 mike@serrratia.demon.co.uk (Michael Gaston) wrote: >It seems likely that the current rapid latex tests for O157 antigen don't >help in the diagnosis of verotoxin producing non-O157 E.coli. I work for a >diagnostic company and we are thinking about developing an immunoassay for >verotoxins. In the first instance this would probably be a >culture-confirmation test rather than a direct faecal screen. > >However, its not clear to me whether a test for verotoxin would be of >sufficient diagnostic value to be useful in a clinical laboratory. If >anyone has experience of using such a test, home-brewed or otherwise, I'd >be greatly interested in their views. > >Thanks in advance, > >Mike > I think a test like this has just been introduced by one or more companies. I think one is Meridian in Ohio. From owner-diagnostics@net.bio.net Fri Apr 14 23:00:00 1995 Path: biosci!biosci.uq.edu.au!fegan From: fegan@biosci.uq.edu.au (Mark Fegan -MICRO) Newsgroups: bionet.diagnostics Subject: Subscription Date: 14 Apr 1995 20:59:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504150358.NAA23220@florey.biosci.uq.oz.au> NNTP-Posting-Host: net.bio.net I would like to subscribe to your discusion group Thanks Mark Fegan | Research Officer | CRC Tropical Plant Pathology | Department of Microbiology |Ph: (07) 3651101 The University of Queensland |FAX:(07) 3654620 From owner-diagnostics@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!SMTPGATE.ADL.HORT.CSIRO.AU!ian.dry From: ian.dry@SMTPGATE.ADL.HORT.CSIRO.AU (Dry, Ian) Newsgroups: bionet.diagnostics Subject: (none) Date: 18 Apr 1995 02:42:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503187982.AA798262814@smtpgate.adl.hort.csiro.au> NNTP-Posting-Host: net.bio.net subscribe diagnost ian dry From owner-diagnostics@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!gen.ks.se!bui From: bui@gen.ks.se (T-H Bui, Clinical Genetics, Karolinska Hospital) Newsgroups: bionet.diagnostics Subject: (none) Date: 18 Apr 1995 06:00:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to subscribe to the newsgroup The-Hung Bui, MD Department of Clinical Genetics Karolinska Hospital, Building L-6, S-171 76 Stockholm, Sweden phone: XX 46 - 8 - 729 4989 or 729 2472 (secr.) fax: XX 46 - 8 - 32 77 34 Email: bui@gen.ks.se or The-Hung.Bui@molmed.ki.se -------------------------------------------------------------------------- From owner-diagnostics@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Morphine and ELISA Date: 18 Apr 1995 15:11:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3min8b$4o5@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net On 13 Apr 1995, Frederic Bourgaud wrote: > Hi all, > > I am starting a new research program dealing with morphine in plants. I am > looking for a rapid/reliable ELISA test that would be adapted to this. I > already know that ELISA tech.(Lexington, Kentucky) commercialises a kit > called "generic opiate ELISA test". It's a competition test and involves > polyclonal antobodies. It is not very specific to morphine as it crosses > with all the related alkaloids. It costs about 90 US $ per plate. > > Does anybody know another ELISA test, maybe based on monoclonal antibodies > ? It is important to me to have a test more specific to morphine. Also any > experience/reference on morphine quantification in plants with ELISA would > be appreciated. > > Also posted to the Biotech and Plant-TC discussion lists. Sorry for > multiple postings. > > Thanks > > Fred (bourgaud@ensaia.u-nancy.fr) For tests for morphine and other drugs of abuse, you can try the following: Hanson Hog Biomedical Co., Taipei, Taiwan, Tel +886-2-7922808, Fax ..7914624 Immunotech Corp, Boston, MA, Tel +1-617-787-1010, Fax ..787-0315 Princeton Biomeditech, Princeton, NJ, Tel +1-908-274-1000, Fax ..274-1010 BiosPacific, Emeryville, CA, Tel +1-415-652-6155, Fax ..652-4531 Eucardio Laboratory, San Diego, CA, Tel +1-619-689-8719, Fax ..689-8719 (The above addresses are from my own database, so some or all may be outdated). As to the specificity of the assay, I think that in your case, less specificity is preferable for initial screening. Once you find a plant with traces of your material, try to determine the exact molecule. Falk Fish, Tel-Aviv, Israel. From owner-diagnostics@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina P. Liu) Newsgroups: bionet.diagnostics Subject: (fwd) IF YOU USE ANTIBODIES, YOU SHOULD READ THIS BULLETIN Date: 18 Apr 1995 09:13:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 217 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Path: condor.ic.net!news.cic.net!newsxfer.itd.umich.edu!caen!uwm.edu!lll-winken.llnl.gov!venus.sun.com!nntp-hub2.barrnet.net!slip.net!usenet From: Bioalert News Newsgroups: sci.bio.technology Subject: IF YOU USE ANTIBODIES, YOU SHOULD READ THIS BULLETIN Date: 17 Apr 1995 06:27:50 GMT Organization: Slip.Net Lines: 206 Distribution: inet Message-ID: <3mt1p6$kuh@slip-1.slip.net> NNTP-Posting-Host: sfsp75.slip.net The information in the following pages should be seen by all the department's scientists. Please, distribute. Electronic Posting Courtesy of BIOALERT NEWS BIOALERT NEWS April 1995 THE FDA ABOUT TO CONTROL ANTIBODIES The Federal Drug Administration, FDA, is nearing its plans to implement a new antibody classification ruling which will designate numerous antibodies as Class II Medical Devices. Many believe that this new regulatory action will have major repercussions in nearly every branch of biological and medical research. The FDA has started with the regulation of antibodies used for immunohistochemistry, but there are good indications to believe that this will expand to cover Flow Cytometry, Hematology, Cytology and even Molecular Biology including DNA/RNA probes. The classification of primary antibodies as Class II Medical Devices was the result of a reclassification petition by the College of American Pathologists (CAP).The meeting of the Hematology and Pathology Devices Panel, was held in Gaithersburg, MD, on October 21, 1994. According to previous FDA rules, these antibodies by default were placed in Class III status. However, this was more a provisional status than a real Device classification, and consequently never enforced. During the Classification Panel meeting the CAP group corroborated their support for Class II as they had previously petitioned. At least five other groups including a dissident group of Immunopathologists within CAP itself, took a position contrary to that of CAP and supported Class I designation. The Joint Council of Immunohistochemistry Manufacturers (JCIM), representing the manufacturers, also supported Class I designation. The JCIM position was also supported by The Health Industry Manufacturers Association (HIMA), the largest association of the health industry with a membership in excess of 700 health care companies. At the end of the meeting, the official group of CAP obtained a victory, and all primary antibodies having clinical application for Immunohistochemistry were recommended to become Class II Medical Devices. No provisions were made to permit the availability of these antibodies for research use. The Panel Classification Meeting of October 21, 1994, was conducted in an unconventional manner. Apparently, during the course of the meeting, the opinions of the delegates of JCIM were suppressed to the extreme that their position paper was not distributed to the Panel members. Furthermore, the manufacturers presentation was kept under strict time control and the manufacturers were not allowed to respond to the arguments of CAP. In contrast, the CAP representation, advocating in favor of Class II, were given unlimited time for delivering their position and for general discussions. This oppressive manner of conducting the meeting as well as several other irregularities of the meeting, prompted the filing of a complaint to the FDA by the Washington law firm of Hyman, Phelps & McNamara, P.C,. on behalf of the oppressed parties. Attorney Jeffrey Gibbs, in his complaint filed with the FDA on January 9, 1995, lists the irregularities, and cites the rules violated during the meeting. The complaint reveals that the FDA officers present during the meeting did not clarify to the members of CAP and the Panel members that the requirements of safety and special controls they were pursuing to accomplish by obtaining Class II could have been implemented even if the antibodies were designated Class I. Essentially, The College of American Pathologists and the Panel members were misled by the FDA into believing that their goals were only possible if Class II was designated. In the complaint, Mr. Gibbs requested that the recommendations of the Panel Meeting be invalidated, the meeting be rescheduled, or the antibodies be Classified as Class I. As of April 10, 1995, three months after receiving the complaint, the FDA has not officially responded. The significance of the outcome of this regulatory petition spearheaded by the College of American Pathologists is that, if designated as Class II Medical Devices, an extensive list of antibodies both polyclonal as well as monoclonal, including CD markers, will be subjected to an expensive process of Class II Medical Device approval. The manufacturers as well as distributors of these antibodies argue that the approval cost of these antibodies will most likely be $20,000 to $30,000 per antibody. They believe that approximately 90% of these antibodies do not produce sufficient sales to justify an expenditure at such level. It is thus believed that many of these antibodies will be removed from availability in the U.S. In addition, all manufacturers and many pathologists assert that these antibodies, when used for immunohistochemistry, are of low-risk to the public and should not merit the strict regulatory environment of Class II Medical Devices. An analysis of this ruling and comparison to the common of Medical Devices controlling products used in Clinical Laboratories, we find a major difference in the magnitude of the scope of the new regulation: In the case of devices for clinical labs, the complete device is the center of the regulatory action. For example, a kit like Hybritech's PSA is classified as a device. This does not designate any antibody to PSA, monoclonal or polyclonal, as a Medical Device. It is the complete kit, the device, which is licensed under the approval process. Therefore, the antibody by itself is not the device. Consequently, antibodies to PSA can be made and sold as antibodies without being an infraction to the FDA regulations. The new ruling, however, is different because it targets the antibodies themselves and gives no provision for the supply of the antibodies outside of the ruling. Under these new regulations, since the PSA antibody has clinical significance for Immunohistochemistry, it becomes itself a Medical Device. Similarly, many other antibodies will be under the same set of rules. In the last two versions of the Points to Consider, a document published by the FDA as a guide to the requirements of this ruling, they have unexpectedly included all auxiliary reagents used in immunohistochemistry as Class II Medical Devices. This was unanticipated since there was no mention or discussion on this subject during the Panel Meeting held on October 21, 1994. Since the classification Panel never ruled on the auxiliary reagents, it is not understood their sudden appearance in the Points to Consider documents. The improprieties of this inclusion may be a separate issue but the significance of this addition goes beyond anything expected. These auxiliary reagents usually contain secondary antibodies labeled with either biotin, peroxidase, alkaline phosphatase as well as conjugates of avidin or streptavidin also conjugated to these tracers, and chromogenic substrates of these enzymes. This ruling designates all of these as Class II Medical Devices. This remains an unassembled puzzle for the manufacturers and many observers. Overall, the amount of antibodies which may be added to this list could be considerable. It will surely include most of the CD antibodies available today as well as antibodies to tumor markers, infectious agents, and practically any antibody of general importance. This appears to be a heavy regulatory weight over a broad range of products that are needed for general research by the U.S. scientific community. Both manufacturers as well as many users fear that this will curtail research in the US and place US scientist at a disadvantage in relation to their European and Japanese colleagues. Some independent Pathologists are disheartened with the idea of increased regulation and fear that the prices of the antibodies will increase considerably. In the midst of this, the prevailing official position of the College of American Pathologists continues to support their original request for these antibodies to be Class II Medical Devices. Altogether, the scope of this new set of rulings led by what appears to be a misled group of Pathologists, is a difficult matter to assess. The detrimental effect it may have on research could be substantial and at this point it is difficult to predict if the gains of this new move will produce benefits commensurate to the loses inflicted to research and indirectly to healthcare. In the mind of many it is difficult to answer, with certainty, the lingering questions: Is this necessary ? Is this really protecting the American public ? Predictable is, that the FDA will continue in their pursuit of controlling biologicals. Whether or not the scientific community will accept the future limitations imposed to their work by the combined efforts and ensuing victory of the College of American Pathologists and the FDA, remains to be seen. Ti H. Sllubon The text of this document is not copyright. Feel free to copy and distribute. Please, help in the distribution of this information. Scientists should be aware of this pending problem. If you have a computer with telecommunication capabilities, download this document and post it to any Life Sciences newsgroups including Medicine. Post under the heading: IF YOU USE ANTIBODIES, READ THIS..... If you get a hardcopy of this document, circulate to all scientific staff at your institution. From owner-diagnostics@net.bio.net Tue Apr 18 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!EU.net!news.sprintlink.net!warp.cris.com!usenet From: jhpincus@cris.com (Jack H. Pincus) Newsgroups: bionet.diagnostics Subject: Re: Reference laboratories Date: 19 Apr 1995 22:56:35 GMT Organization: Concentric Research Corporation Lines: 28 Distribution: world Message-ID: <3n44f3$5lo@warp.cris.com> References: <9504190113.AA04997@Rt66.com> NNTP-Posting-Host: crc1.cris.com Mime-Version: 1.0 X-Newsreader: WinVN 0.93.11 In article <9504190113.AA04997@Rt66.com>, ingen@RT66.COM says... > >Hellow netters, > >I have a dumb question. Could somebody explain what is a "reference" >laboratory? I did not pay any attention to this particular term. > >Does a reference laboratory require certification by state or federal >government? I am curious. > >Thanks. > >In C. Kim, Ph.D. > The term is something of a misnomer. Reference laboratories perform tests that are not routinely done in clinical laboaratories. Tests performed by reference laboratories are sometimes more complicated than routine clincal laboartory assays, or those for which there is not enough demand. In the U.S. states regualte clinical laboratories. Most, if not all, require some sort of licensure or certification. Reference laboratories are no exception. Jack H. Pincus jhpincus@cris.com From owner-diagnostics@net.bio.net Tue Apr 18 23:00:00 1995 Path: biosci!BCRSSU.AGR.CA!RAMPITSCH From: RAMPITSCH@BCRSSU.AGR.CA Newsgroups: bionet.diagnostics Subject: Freezing thymocytes: possible? Date: 19 Apr 1995 14:49:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HPJ5KB4WMQ000PVX@GW.AGR.CA> NNTP-Posting-Host: net.bio.net I am planning to use mouse thymocytes as feeder cells for hybridomas. The thymus donors are now a month old, but the fusion... well I haven't even started immunizing yet. I was wondering whether it is possible to freeze feeder cells and thaw them a day or so before the fusion. I'd really rather use thymocytes than spleenocytes, so I have to decide on this within the next week or so. Please let me know if you've tried this, how it worked, protocols, etc. Thanks, Chris From owner-diagnostics@net.bio.net Tue Apr 18 23:00:00 1995 Path: biosci!RT66.COM!ingen From: ingen@RT66.COM Newsgroups: bionet.diagnostics Subject: Reference laboratories Date: 18 Apr 1995 18:14:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504190113.AA04997@Rt66.com> NNTP-Posting-Host: net.bio.net Hellow netters, I have a dumb question. Could somebody explain what is a "reference" laboratory? I did not pay any attention to this particular term. Does a reference laboratory require certification by state or federal government? I am curious. Thanks. In C. Kim, Ph.D. From owner-diagnostics@net.bio.net Wed Apr 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!nntp.crl.com!decwrl!svc.portal.com!news1.best.com!alexon.vip.best.com!user From: alexon@best.com (Alexon) Newsgroups: bionet.diagnostics,bionet.virology Subject: Rotavirus & Adenovirus Date: Wed, 19 Apr 1995 17:29:34 -0800 Organization: Alexon Biomedical Lines: 6 Message-ID: NNTP-Posting-Host: alexon.vip.best.com Xref: biosci bionet.diagnostics:87 bionet.virology:2330 I am interested in talking to anyone with Rotavirus <+> or Adenovirus <+> samples (confirmed by "EM"), MAb and PAb (for above). Also, does anyone know the whereabouts of Dr. Ken Hermann (last known address was in Mass.). Please reply to alexon@best.com subject David McCarty. From owner-diagnostics@net.bio.net Wed Apr 19 23:00:00 1995 Path: biosci!rutgers!gatech!swrinde!pipex!sunsite.doc.ic.ac.uk!yama.mcc.ac.uk!usenet From: rgerke-b@mh1.mcc.ac.uk (Stopford Building Computer User) Newsgroups: bionet.molbio.genome-program,bionet.genome.chromosomes,bionet.molbio.gene-linkage,bionet.diagnostics,bionet.cellbiol,bionet.cellbiol.cytonet Subject: Robotic colony pickers or griders Date: 20 Apr 1995 14:12:13 GMT Organization: Manchester University Lines: 13 Message-ID: <3n5q3t$8ei@yama.mcc.ac.uk> NNTP-Posting-Host: rger.sbc.man.ac.uk Mime-Version: 1.0 X-Newsreader: WinVN 0.99.2 Xref: biosci bionet.molbio.genome-program:1342 bionet.genome.chromosomes:555 bionet.molbio.gene-linkage:651 bionet.diagnostics:88 bionet.cellbiol:2112 bionet.cellbiol.cytonet:212 Does anyone can probide me with comments or information regarding the Hybaid robotic colony picker or the grider. We are contemplating buying one and we will apreciate very much any comments. Please respond by e-mail to rgerke-b@mh1.mcc.ac.uk Thank you very much Roger Gerke-Bonet From owner-diagnostics@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!agate!news.mindlink.net!news.bc.net!unixg.ubc.ca!137!wjia From: wjia@unixg.ubc.ca (William) Newsgroups: bionet.diagnostics Subject: URGENT, HELP NEEDED! Followup-To: bionet.diagnostics Date: Fri, 21 Apr 95 08:26:30 GMT Organization: The University of British Columbia Lines: 62 Message-ID: NNTP-Posting-Host: imagination.ecc.ubc.ca X-Newsreader: VersaTerm Link v1.1.1 For all, please read and forward to as many people as possible. This person desperately needs help. The more this circulates, the better chance that someone who knows what to do will be found. Thanks! -- Hi, This is Peking University in China, a place those dreams of freedom and democracy. However, a young, 21-year old student has become very sick and is dying. The illness is very rare. Though they have tried, doctors at the best hospitals in Beijing cannot cure her; may do not even know what illness it is. So now we are asking the world -- can somebody help us? Here is a description of the illness: The young woman -- her name is Zhu Ling -- is a student in the chemistry department. On DEC. 5, 1994, Zhu Ling felt sick to her stomach. Three days later, her hair began to fall out and within two days she was completely bald. She entered the hospital, but doctors could not discover the season for her illness. However, after she was in the hospital for a month, she began to fell better and her hair grew back. Zhu Ling went back to school in February, but in March her legs began to ache severely, and she felt dizzy. She entered XieHe Hospital - Chinese most famous hospital. In early March and on March 15, her symptoms worsened. She Began to facial paralysis, central muscle of eye's paralysis, self- controlled respiration disappeared. So she was put on a respirator. The doctors did many tests for many diseases(include anti- H2V, spinal cord puncture, NMR, immune system, chemical drug intoxication ANA,ENA,DSONA,ZG and Lyme), but all were negative, except for Lyme disease(ZGM(+)). The doctors now think that it might be acute disseminated encephalomyelitis(ADEM) or lupus erythematosus(LE), but the data from the tests do not support this conclusion. The doctors are now treating Zhu Ling with broad-spectrum antibiotic of cephalosporin, anti-virus drug, hormone, immun- oadjuvent, gamma globulin intravenous injection and have given her plasma exchange(PE) of 10,000 CCs. But Zhu Ling has not responded -- she reamers in a vegetative state, sustained by life support. If anyone has heard of patients with similar symptoms -- or have any ideas as to what this illness could be, please contact us. We are Zhu Ling's friends and we are disparate to help her. This is the first time that Chinese try to find help from Internet, please send back E-mail to us. We will send more crystal description of her illness to you. Thank you very much Peking University April 10th, 1995 ====================================================================== Please foreword this message to your freinds if you think they can be helpful. Thanks in advance! email:caiqq@mccux0.mech.pku.edu.cn From owner-diagnostics@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: lambda phage mabs Date: 21 Apr 1995 08:01:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net THis is my second attempt at this message - the first seemed to vanish into the ether. Sorry if it is duplicated. Hi all, have any of you had a go at generating antibodies using lambda phage expression vectors. I know that one main advantage is the numbers of antibodies generated but what are the disadvantages? Thirdly does anyone know of an easy-to-use kit which can be used for this technology? Thanks Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!UNIXG.UBC.CA!lingqin From: lingqin@UNIXG.UBC.CA (Xiang Li) Newsgroups: bionet.diagnostics Subject: asking for medical help from Beijing (fwd) Date: 20 Apr 1995 19:58:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 70 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Here is a mail from China. The patient and her friends and family will appreciate for your help indeed. Xiang Li Agriculture Canada, Pacific Agriculture Research Centre--Vancouver ---------- Forwarded message ---------- Date: Thu, 20 Apr 1995 21:38:40 -0400 (EDT) From: JZHAO@mitlns.mit.edu To: ZHONG_HUA@cs.ubc.ca Subject: asking for help from Beijing Subject: SOS!!! For all, please read and forward to as many people as possible. This person desperately needs help. The more this circulates, the better chance that someone who knows what to do will be found. Thanks! - ------------------------------------------------------------------- Hi, this is Peking University in China, a place those dreams of freedom and democracy. However, a young, 21-year old student has become very sick and is dying. The illness is very rare. Though they have tried, doctors at the best hospitals in Beijing cannot cure her; may do not even know what illness it is. So now we are asking the world -- can somebody help us? Here is a description of the illness: The young woman -- her name is Zhu Ling -- is a student in the chemistry department. On DEC. 5, 1994, Zhu Ling felt sick to her stomach. Three days later, her hair began to fall out and within two days she was completely bald. She entered the hospital, but doctors could not discover the season for her illness. However, after she was in the hospital for a month, she began to fell better and her hair grew back. Zhu Ling went back to school in February, but in March her legs began to ache severely, and she felt dizzy. She entered XieHe Hospital - Chinese most famous hospital. In early March and on March 15, her symptoms worsened. She Began to facial paralysis, central muscle of eye's paralysis, self-controlled respiration disappeared. So she was put on a respirator. The doctors did many tests for many diseases(include anti- H2V, spinal cord puncture, NMR, immune system, chemical drug intoxication ANA,ENA,DSONA,ZG and Lyme), but all were negative, except for Lyme disease(ZGM(+)). The doctors now think that it might be acute disseminated encephalomyelitis(ADEM) or lupus erythematosus(LE), but the data from the tests do not support this conclusion. The doctors are now treating Zhu Ling with broad-spectrum antibiotic of cephalosporin, anti-virus drug, hormone, immun- oadjuvent, gamma globulin intravenous injection and have given her plasma exchange(PE) of 10,000 CCs. But Zhu Ling has not responded -- she reamers in a vegetative state, sustained by life support. If anyone has heard of patients with similar symptoms -- or have any ideas as to what this illness could be, please contact us. We are Zhu Ling's friends and we are disparate to help her. This is the first time that Chinese try to find help from Internet, please send back E-mail to us. We will send more crystal description of her illness to you. Thank you very much Peking University April 10th, 1995 ========================================================================== Please foreword this message to your freinds if you think they can help us ,Thanks advanced! email:caiqq@mccux0.mech.pku.edu.cn ------- End of Forwarded Messages From owner-diagnostics@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina P. Liu) Newsgroups: bionet.diagnostics Subject: (fwd) Infor About Zhu Ling's illness:from her freinds Date: 21 Apr 1995 12:10:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Path: condor.ic.net!news.cic.net!infoserv.illinois.net!news.nd.edu!spool.mu.edu!howland.reston.ans.net!agate!hpg30a.csc.cuhk.hk!linuxguy.pku.edu.cn!mccux0!caiqq From: caiqq@mccux0.mech.pku.edu.cn (Cai Quanqing) Newsgroups: sci.med,sci.med.dentistry,sci.med.diseases.cancer,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.occupational,sci.med.pharmacy,sci.med.physics,sci.med.psychobiology,sci.med.radiology,sci.med.telemedicine,sci.med.transcription,sci.med.vision Subject: Infor About Zhu Ling's illness:from her freinds Date: 19 Apr 1995 15:15:06 GMT Organization: Peking Universary,China Lines: 36 Message-ID: <3n39dq$5bj@linuxguy.pku.edu.cn> NNTP-Posting-Host: 162.105.195.2 X-Newsreader: TIN [version 1.2 PL2] Xref: condor.ic.net sci.med:82618 sci.med.dentistry:5500 sci.med.diseases.cancer:1901 sci.med.immunology:1095 sci.med.informatics:1894 sci.med.nursing:4076 sci.med.nutrition:21979 sci.med.occupational:2941 sci.med.pharmacy:8341 sci.med.physics:2879 sci.med.psychobiology:3656 sci.med.radiology:1806 sci.med.telemedicine:4859 sci.med.transcription:997 sci.med.vision:3636 Hi : We cannot believe so many mails we have gotten. Thank you very much for your generous help. Now we are busy in translating them to Chinese and send them to PUMC(Peking Union Medical College) hospital. We are sorry that we can not send mail back one by one. The following messages try to answer some common questions and tell you some new information. 1. The doctors still don't believe Zhu Ling's illness is heavy metal poison or Lyme disease. 2. Most of the doctors think the illness would be Guillan-Barre syndrome. Zhu Ling has been in coma since Mar 15, The CSF was normal in Mar 12 and Mar 22, but abnormal in April 12. The doctors in PUMC cannot explain that. 3. We are trying to get the data that you want to know. We will send them back as soon as we get the data. 4. We believe that using tele-medicine conference system to diagnose will be helpful to Zhu Ling . If you want to get any more information or give more help (include financial support), You can contact Dr. John W. Aldis, His E-mail address is: jwaldis@ix.netcom.com 5. If you want to contact with us, You can fax to Ms. Bei Lu Ying ( 86-10-8502728), You can also phone her ( 86-10-8502117 )at Greenwich Time 00:00-03:30 and 06:00-09:30. 6. Our address is: 32# Room 134 Peking University, Beijing, China PostCode: 100871 You can send package or letter to Mr. Liu Li. Thank you very much Zhu Ling's friends Peking University April 17, 1995 From owner-diagnostics@net.bio.net Fri Apr 21 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: lambda phage mabs Date: 22 Apr 1995 00:06:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On 21 Apr 1995, Robert Burns wrote: > > Hi all, > have any of you had a go at generating antibodies using > lambda phage expression vectors. I know that one main advantage > is the numbers of antibodies generated but what are the disadvantages? > Thirdly does anyone know of an easy-to-use kit which can be used for > this technology? > > Thanks > > Robert > > > > > > Robert Burns > The main problem of this method is the low affinity of the resulting antibodies. I did not have direct experience with the method but we keep it in the back of our minds as a viable strategy to generate antibodies, while combining it with an approach to ensure that the affinity will be usably high. As to a kit: I think Pharmacia offers a kit. I am not sure and I will get back after looking at my papers. You could also contact the guys at Cambridge: they have invented it all. Falk Fish, Tel-Aviv, Israel From owner-diagnostics@net.bio.net Sat Apr 22 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!usenet.ufl.edu!usenet From: Bob Ouyang Newsgroups: bionet.diagnostics Subject: S.O.S FOR A DYING YOUNG CHINESE GIRL Date: 23 Apr 1995 20:03:27 GMT Organization: University of Florida Lines: 83 Message-ID: <3nebqf$9f2@no-names.nerdc.ufl.edu> NNTP-Posting-Host: ppp-01-nerdc-ts3.nerdc.ufl.edu Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1b3 (Windows; I; 16bit) From: edu%"cqyang@chemistry.umass.edu" "Dr. C.-Q. Yang" 22-APR-1995 17:45:43.01 To: Multiple recipients of list CHINA-NT CC: Subj: Cry for Help From Peking University Received: From NERVM(MAILER) by UFOAK with Jnet id 0135 for OY8930@UFCC; Sat, 22 Apr 1995 17:45 EST Return-Path: Received: from NERVM.NERDC.UFL.EDU (NJE origin LISTSERV@NERVM) by NERVM.NERDC.UFL.EDU (LMail V1.2a/1.8a) with BSMTP id 0274; Sat, 22 Apr 1995 17:46:00 -0400 Date: Sat, 22 Apr 1995 17:45:44 -0400 Reply-To: "Dr. C.-Q. Yang" Sender: China-Net From: "Dr. C.-Q. Yang" Subject: Cry for Help From Peking University Comments: To: China-Nt To: Multiple recipients of list CHINA-NT Dear Friends, The following message was sent from Peking University. Please send your response to caiqq@mccux0.mech.pku.edu.cn if you can offer any help. Many thanks! Forwarded message: > > -- > > Hi, > This is Peking University in China, a place those dreams of > freedom and democracy. However, a young, 21-year old student > has become very sick and is dying. The illness is very rare. Though > they have tried, doctors at the best hospitals in Beijing cannot cure > her; may do not even know what illness it is. So now we are asking > the world -- can somebody help us? > Here is a description of the illness: > The young woman -- her name is Zhu Ling -- is a student in > the chemistry department. On DEC. 5, 1994, Zhu Ling felt sick to > her stomach. Three days later, her hair began to fall out and within > two days she was completely bald. She entered the hospital, but > doctors could not discover the season for her illness. However, > after she was in the hospital for a month, she began to fell better > and her hair grew back. Zhu Ling went back to school in February, > but in March her legs began to ache severely, and she felt dizzy. > She entered XieHe Hospital - Chinese most famous hospital. In > early March and on March 15, her symptoms worsened. She Began > to facial paralysis, central muscle of eye's paralysis, self-controlled > respiration disappeared. So she was put on a respirator. > The doctors did many tests for many diseases(include anti- > H2V, spinal cord puncture, NMR, immune system, chemical drug > intoxication ANA,ENA,DSONA,ZG and Lyme), but all were > negative, except for Lyme disease(ZGM(+)). > The doctors now think that it might be acute disseminated > encephalomyelitis(ADEM) or lupus erythematosus(LE), but the > data from the tests do not support this conclusion. > The doctors are now treating Zhu Ling with broad-spectrum > antibiotic of cephalosporin, anti-virus drug, hormone, immun- > oadjuvent, gamma globulin intravenous injection and have given > her plasma exchange(PE) of 10,000 CCs. But Zhu Ling has not > responded -- she reamers in a vegetative state, sustained by life > support. > If anyone has heard of patients with similar symptoms -- or > have any ideas as to what this illness could be, please contact us. > We are Zhu Ling's friends and we are disparate to help her. > This is the first time that Chinese try to find help from > Internet, please send back E-mail to us. We will send more crystal > description of her illness to you. > > Thank you very much > Peking University > April 10th, 1995 > ========================================================================== > Please foreword this message to your freinds if you think they can help > us ,Thanks advanced! > > email:caiqq@mccux0.mech.pku.edu.cn > > > > > ----- End Included Message ----- From owner-diagnostics@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!WADSWORTH.ORG!woodall From: woodall@WADSWORTH.ORG Newsgroups: bionet.diagnostics Subject: Re: fw:"Good Times" Computer Virus: No panic Date: 24 Apr 1995 08:32:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504241532.LAA00470@wadsworth.ph.albany.edu> NNTP-Posting-Host: net.bio.net At 07:17 AM 4/24/95-0700, Bart_Corsaro_at_USLRMG01@internetmail.pr.cyanamid.com wrote: > >VIRUS ALERT: FOR ALL INTERNET USERS. > Don't panic: there's no way a computer virus can be sent by e-mail. Viruses can only travel in programs, not data, and e-mail is data. [If you are downloading programs from the net, that's a different matter.] Jack -- Jack Woodall, ProMED List Moderator, NY State Dept. of Health, Albany NY e-mail: woodall@wadsworth.org From owner-diagnostics@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!INTERNETMAIL.PR.CYANAMID.COM!Bart_Corsaro_at_USLRMG01 From: Bart_Corsaro_at_USLRMG01@INTERNETMAIL.PR.CYANAMID.COM Newsgroups: bionet.diagnostics Subject: fw:"Good Times" Computer Virus Date: 24 Apr 1995 07:17:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Distribution: world Message-ID: <9503247987.AA798743635@internetmail.pr.cyanamid.com> NNTP-Posting-Host: net.bio.net VIRUS ALERT: FOR ALL INTERNET USERS. ======================================================================= ======== Date: 04/19/1995 08:44 am (Wednesday) From: Ryan Wheat Subject: Internet Virus The following message was sent to me late last night via the InterNet. PLEASE READ CAREFULLY! ----------------------------------------------------------------------- ---------------- There is a new computer virus that is being sent across the Internet. If you receive an e-mail message with the subject line "Good Times", DO NOT read the message, DELETE it immediately. Please read the messages below. Someone is sending e-mail under the title "Good Times" nation-wide. If you get anything like this, DON'T DOWNLOAD THE FILE! It has a virus that rewrites you hard drive, obliterating anything on it. Please be careful and forward this mail to anyone you care about. Apparently , a new computer virus has been engineered by a user of America Online that is unparalled in it's destructive capability. Other, more well-known viruses such as Stoned, Airwolf and Michaelangelo pale in comparison to the prospects of this newest creation by a warped mentality. The bottom line here is - if you receive a file with the subject line "Good Times", delete it immediately! DO NOT READ IT! Rest assured that whoever's name was on the "From" line was surely struck by the virus. Warn you friends and local system users of this newest threat to the InterNet. It could save them a lot of time and money. ----------------------------------------------------------------------- ---------------- This was an abbreviated version of what was sent to me. If you would like additional information as to what would happen if "Good Times" was downloaded, please reply back. Please pass this on. Thanks, Ryan Wheat From owner-diagnostics@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!torn!news.bc.net!rover.ucs.ualberta.ca!news.ucalgary.ca!pc22.mid.ucalgary.ca!user From: macklon@acs.ucalgary.ca (Peter Macklon) Newsgroups: bionet.diagnostics Subject: Re: fw:"Good Times" Computer Virus Date: Mon, 24 Apr 1995 14:51:43 -0600 Organization: Microbiology and Infectious Diseases, U of C Lines: 16 Distribution: world Message-ID: References: <9503247987.AA798743635@internetmail.pr.cyanamid.com> NNTP-Posting-Host: pc22.mid.ucalgary.ca This alert is bogus. It has already made the rounds some months ago. When you think about it, how could a text (non binary, no UUencoded contents that are decoded) document contain virus code that would infect your system by itself? ****************************** > There is a new computer virus that is being sent across the Internet. > If > you receive an e-mail message with the subject line "Good Times", DO > NOT read the message, DELETE it immediately. Please read the > messages below. ******************************* Rest easy. Peter From owner-diagnostics@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!UNIXG.UBC.CA!lingqin From: lingqin@UNIXG.UBC.CA (Xiang Li) Newsgroups: bionet.diagnostics Subject: Forward: The virus message was a hoax! (fwd) Date: 24 Apr 1995 09:20:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, there. I read the same message sent by Ryan Wheat before from another friend. I had also received the following message afterward. Just for your information. Good luck. Xiang Li Agriculture Canada, Pacific Agriculture Research Centre--Vancouver ---------- Forwarded message ---------- Date: Thu, 20 Apr 1995 20:05:07 PST From: James J. Pan To: zhong_hua@cs.ubc.ca Cc: jjpan@erich.triumf.ca Subject: The virus message was a hoax! (fwd) Sorry for the false alarm. jp > Date: 19 Apr 95 11:11 -0700 > From: William Lee > To: Branko Peric > Cc: net-admins@ubc.ca > Subject: Re: (Fwd) WARNING!! > > On Wed, 19 Apr 1995, Branko Peric wrote: > > > You-All heard about this? > > > > ------- Forwarded Message Follows ------- > > > > >> FWD>F.Y.I. New Computer Virus > > >> > > >> There is a computer virus that is being sent across the Internet. If you > > >> receive an e-mail message with the subject line "Good Times", DO NOT read > > >> the message, DELETE it immediately. Please read the messages below. > > [a pile of nonsense deleted] > > This is a hoax. There was something similar a couple of months back. I > can dig up the CERT (or somebody like that's) rebuttal if anyone is > interested. > > Some key [ridiculous] claims: > > 1) the 'FCC' won't care about viruses > 2) you can't 'destroy' a modern hard drive with a virus, perhaps the data, > but not the hard drive > 3) no n-th complex binary loop or whatever he said can destroy a processor > > > **Now this is not to say that you can't get a virus via email. Someone can > send you an infected executable that when downloaded and run can infect > your hard drive. But simply 'reading' a message won't infect anything. > > William. > > ---- > William Lee, Manager, Systems Consulting Group > Microcomputer, Unix Systems and Network Consulting > Network Systems Support, Telecommunications Services > Computing and Communications, University of British Columbia > 6356 Agricultural Road, Vancouver, BC V6T 1Z2 Canada. email: lee@ucs.ubc.ca > --- James J. Pan JJPAN@triumf.ca (604)222-1047 UBC & TRIUMF JJPAN@unixg.ubc.ca (604)822-8243 Vancouver, BC CANADA V6T 1A3 From owner-diagnostics@net.bio.net Sun Apr 23 23:00:00 1995 Path: biosci!ISNET.IS.WFU.EDU!bmorrell From: bmorrell@ISNET.IS.WFU.EDU (Bob Morrell) Newsgroups: bionet.diagnostics Subject: Re: Internet VIRUS (fwd) Date: 24 Apr 1995 08:56:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The "good times virus" was a hoax virus scare from several months back. There is no way that reading email can introduce a virus.... Like many things on the Internet, this issue seems to resurect itself periodically as people stumble across old email, or newbies on the net see references to a problem, without seeing references to its resolution. You can add the good times virus to the FCC idea of modem surcharges, the 200$ cookie recipe, and the boy who want=ed= get well cards. You should not, however, feel bad about falling for one of these. Everyone does (I have my letter from a very frustrated FCC bureacrat) and one of the hazards of the net is that it is very difficult to distinguish these things. My rule of thumb: read all of my mail before sending out anything. Usually in a package of 20 emails, the answer to number 2 will be found in number 15 or so. * Bob Morrell * * bmorrell@isnet.is.wfu.edu * * The operation was a success, as the autopsy will show * From owner-diagnostics@net.bio.net Mon Apr 24 23:00:00 1995 Path: biosci!reading.ac.uk!M.W.Shaw From: M.W.Shaw@reading.ac.uk ("Michael W. Shaw") Newsgroups: bionet.diagnostics Subject: (none) Date: 25 Apr 1995 03:43:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe diagnost From owner-diagnostics@net.bio.net Tue Apr 25 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina Liu) Newsgroups: bionet.diagnostics Subject: National Cancer Institute--Collaborations in Technology Development Date: 26 Apr 1995 12:54:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 106 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I thought people would be interested in this announcement that I found today on NewsPage. Best regards, Christina ****************************************************************************** > [storytop] > ------------------------------------------------------------------------ > > > National Cancer Institute/ Opportunity for a Cooperative Research and > Development Agreement (CRADA) for the Scientific and Commercial > Development of Monoclonal Antibodies for the Therapy and/or Diagnosis > of Cancer > > ------------------------------------------------------------------------ > > > Source: The Federal Register > > The Federal Register via NewsPage : DEPARTMENT OF HEALTH AND HUMAN > SERVICES > > National Institutes of Health, PHS, DHHS. > > National Cancer Institute: Opportunity for a Cooperative Research and > Development Agreement (CRADA) for the Scientific and Commercia l > Development of Monoclonal Antibodies for the Therapy and/or Diagnosis > of Cancer > > ACTION: Advertisement. > > SUMMARY: The Laboratory of Tumor Immunology and Biology (LTIB), > National Cancer Institute is seeking pharmaceutical or biotechnology > collaborator(s) which can effectively pursue the scientific and > commercial development of a panel of monoclonal antibodies generated > against tumor associated antigens for use in the therapy and/or > diagnosis of a range of human cancers. The primary focus of these > collaborations will be the development and commercialization of a > panel of monoclonal antibodies consisting of two major groups: (A) > Monoclonal antibodies directed against the pancarcinoma antigen, > TAG-72. TAG-72 is expressed on a range of human carcinomas including > colorectal, gastric, pancreatic, ovarian, endometrial, breast, > non-small cell lung, and prostate. Monoclonal antibody CC49 is the > prototype monoclonal antibody of this group. Humanized and other > genetically engineered variants of monoclonal antibody CC49 have > already been developed. (B) Monoclonal antibodies directed against > human carcinoembryonic antigen, which is expressed on the following > carcinomas: colorectal, pancreatic, gastric, non-small cell lung, and > breast carcinoma. The prototype for this group of monoclonal > antibodies is COL-1. (C) Additionally, it may likely be a further goal > of these collaborations to develop novel recombinant forms of these > monoclonal antibodies. > > It is anticipated that because of the magnitude, diversity, and > expense of these proposed research projects the collaboration(s) may > take the form of multiple CRADAs. The collaboration(s) will involve > all aspects of diagnostic and/or therapeutic development from basic > scientific inquiry to late stage clinical trials which selected > sponsor(s) will be required to partially support. The selected > sponsor(s) will collaborate in the development of one or more of the > following diagnostic or therapeutic forms of these monoclonal > antibodies: (1) Radiolabeled monoclonal antibodies (diagnostic > (oncologic imaging) and/or therapeutics); (2) Drug and/or toxin > conjugated monoclonal antibodies; (3) Pro-drug conjugated monoclonal > antibodies; (4) Unconjugated monoclonal antibodies (including > bifunctional forms). > > Sponsors will be selected based upon their ability to collaborate with > NCI for the development of any of these therapeutic or diagnostic > forms in accordance with the corporate role and selection criteria > outlined below. It is emphasized that selection of a collaborator will > not be dependent upon an entity's ability to perform the largest > portion of the research project. Rather, a collaborator will be > selected based upon the scientific merit and intellectual > contributions brought to each individual project(s). Potential > collaborators are, therefore, urged to submit proposals which focus on > particular area(s) of expertise in a well-organized and precise manner > which clearly outlines a development and commercialization plan. > Finally, it is also possible that logical extensions of these research > protocols may be considered as potential collaborative projects. > Accordingly, proposals must address the requested criteria and > protocols, but in addition, may include any additional unique > development projects relating to the core technology. > > The term of the CRADA(s) is anticipated to be three (3) to five (5) > years. > > ADDRESSES: Inquiries and proposals regarding this opportunity should > be addressed to either Michael Christini or Mark Noel (Tel > #301-496-0477, Fax #301-402-2117), Office of Technology Development, > National Cancer Institute, Building 31, Room 4A49, NIH, 9000 Rockville > Pike, Bethesda, MD 20892. > > DATES: Proposals must be received at the above address by 5 p.m. June > 26, 1995. > > [04-25-95 at 18:20 EDT, Counterpoint Publishing] > > ------------------------------------------------------------------------ > > Feedback |Main |Index |Map |SurfNews |Copyright 1995 |Individual, Inc. > | From owner-diagnostics@net.bio.net Wed Apr 26 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!swrinde!pipex!warwick!news.ncl.ac.uk!usenet From: Simon Eaton Newsgroups: bionet.metabolic-reg,bionet.diagnostics,bionet.general Subject: Dept Child Health,Metabolic Unit Home Page Date: 27 Apr 1995 08:21:54 GMT Organization: Newcastle University, UK Lines: 9 Message-ID: <3nnk72$sec@whitbeck.ncl.ac.uk> NNTP-Posting-Host: russet.ncl.ac.uk X-Newsreader: WinVN 0.91.6 Xref: biosci bionet.metabolic-reg:463 bionet.diagnostics:102 bionet.general:14862 The Department of Child Health, University of Newcastle-upon-Tyne now has a WorldWideWeb page. This is initially dedicated to the Metabolic Unit but will soon include links to the other Research Groups within the Department. It is found at: http://www.ncl.ac.uk/~nchwww Simon Eaton Research Associate From owner-diagnostics@net.bio.net Wed Apr 26 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina Liu) Newsgroups: bionet.diagnostics Subject: More news on NCI's CRADA Date: 27 Apr 1995 06:03:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net > [storytop] > ------------------------------------------------------------------------ > > > RESEARCH/ National Cancer Institute CRADA. > > ------------------------------------------------------------------------ > > > Source: Health News Daily > > Health News Daily via NewsPage : National Cancer Institute CRADA: > NCI's Laboratory of Tumor Immunology and Biology seeking partner for > Cooperative Research & Development Agreement for a "panel of > monoclonal antibodies generated against tumor-associated antigens for > use in the therapy and/or diagnosis of a range of human cancers," NCI > announces in the April 25 Federal Register. Two major MAb groups are > the focus: MAbs "directed against the pancarcinoma antigen, TAG-72," > which is expressed in colorectal, gastric, pancreatic, ovarian, > endometrial, breast, non-small cell lung and prostate cancers, to name > some. CC49 is the prototype Mab for this group, NCI says, and > humanized and other genetically altered variants already have been > developed. The second MAb group is "directed against human > carcinoembryonic antigen, which is expressed in the following > carcinomas: colorectal, pancreatic, gastric, non-small cell lung and > breast carcinoma." The prototype, NCI says, is COL-1. NCI believes the > magnitude of the project may require multiple CRADAs, the terms of > which are anticipated to be three to five years. Proposals must be > received by June 5. Contact Michael Christini or Mark Noel at > 301/496-0477 for further information. > > [04-25-95 at 19:18 EDT, Copyright 1995, F-D-C Reports, Inc.] > > ------------------------------------------------------------------------ > > Feedback |Main |Index |Map |SurfNews |Copyright 1995 |Individual, Inc. > | From owner-diagnostics@net.bio.net Thu Apr 27 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Self-Sustained Sequence replication Organization: Scottish Agricultural Science Agency Date: Fri, 28 Apr 1995 10:51:13 GMT Message-ID: X-Newsreader: WinVN 0.91.6 Sender: news@jura.sasa.gov.uk (Usenet) Lines: 15 Is anybody using SSSR* for anything? How does it compare to PCR/LCR in terms of sensitivity , specificity and ease of use? also, can anyone explain the difference between SSSR (non-patented) and the to my eyes very similar NASBA (nucleic acid sequence based amplification) which is patented> *self-sustained sequence replication entails amplification by RNA transcription, for anyone who's wondering what I'm on about. Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland From owner-diagnostics@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!MED.PITT.EDU!ehr From: ehr@MED.PITT.EDU (Garth Ehrlich) Newsgroups: bionet.diagnostics Subject: Re: Self-Sustained Sequence replication Date: 28 Apr 1995 07:25:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504281323.AA04815@venus.med.pitt.edu> NNTP-Posting-Host: net.bio.net 3SR and NASBA are essentially identical. Both were based upon Tom Gingeras's original RNA amplification system TAS (transcript amplification system). Tom then went on to develop 3SR, but in an ironic twist of fate got beaten to the patent by Organon Teknika which independently developed NASBA from his TAS system. If your interested in the original description of TAS it was published in Science in 1986. You're welcome ot send this out to the net if you so desire. Sincerely Garth D, Ehrlich, Ph.D> Director, Core PCR Facility University of Pittsburgh Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Sat Apr 29 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!crash!ideal11.ios.net!merlin From: merlin@ideal.ios.net Subject: The Virtual Marketplace Organization: Ideal Online Services Date: Sun, 30 Apr 1995 05:55:51 GMT Message-ID: X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: ideal11.ios.net Lines: 18 Hello! I designed the Virtual Marketplace because I wanted a cybermall that not only everyone could come to shop hassle free in, but was within the means of most businesses to advertise on as well. 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