From owner-diagnostics@net.bio.net Sun May 07 23:00:00 1995 Path: biosci!CCLINK.FHCRC.ORG!jcarter From: jcarter@CCLINK.FHCRC.ORG (jcarter at FH2) Newsgroups: bionet.diagnostics Subject: prism Date: 8 May 1995 10:40:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone have experience with the prism graphics and statistical progams made by the graphpad company? I want to use them for analysis of ELISA data. From owner-diagnostics@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!agate!library.ucla.edu!news.bc.net!rover.ucs.ualberta.ca!news.ucalgary.ca!news From: dchurchi@acs.ucalgary.ca (DENNIS CHURCHILL) Newsgroups: bionet.diagnostics Subject: Re: prism Date: 9 May 1995 14:36:22 GMT Organization: University of Calgary Lines: 8 Message-ID: <3onul6$1pvu@ds2.acs.ucalgary.ca> References: NNTP-Posting-Host: pc5.macvicar.ucalgary.ca X-Newsreader: WinVN 0.92.6+ In article , jcarter@CCLINK.FHCRC.ORG (jcarter at FH2) says: > > Does anyone have experience with the prism graphics and statistical > progams made by the graphpad company? I want to use them for analysis > of ELISA data. I have been using Prism for eight months now. It was very easy to learn and does an outstanding job! I highly recommend it. From owner-diagnostics@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!rutgers!concert!gatech!howland.reston.ans.net!news.sprintlink.net!news.clark.net!NewsWatcher!user From: kcowing@aibs.org (Keith L. Cowing) Newsgroups: bionet.immunology,bionet.diagnostics Subject: PROPOSAL SOLICITATION: US Army: Epidemiological Studies Persian Gulf War Illnesses (PGI-1) Date: Tue, 09 May 1995 14:27:27 -0500 Organization: American Institute of Biological Sciences Lines: 56 Message-ID: NNTP-Posting-Host: kcowing-ppp.clark.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit Xref: biosci bionet.immunology:4061 bionet.diagnostics:109 AIBS NOTE: No conventional solicitation will be issued by the Army for this action. Updates will be posted to various newsgroups if/when they are received by AIBS. For updates on this and other biomedical RFPs, proposal solicitations, fellowships, and research opportunities, check the AIBS Gopher at gopher.aibs.org or via WWW at gopher://aibs.org - updates will appear in the PROFESSIONAL OPPORTUNITIES folder. +++++ RECORD NUMBER: 1342PRA0008 CBD ISSUE DATE: 5/9/95 SECTION: Procurements FSC CODE: A TITLE: R&D STREAMLINED CONTRACT SOL DAMD17-95-#-0008 DUE 052495 Point of Contact: CRAIG D. LEBO, 301-619-2036 Epidemiological Studies Persian Gulf War Illnesses (PGI-l) The U.S. Army Medical Research and Materiel Command (USAMRMC) on behalf of the Departments of Defense, Veteran's Affairs and Health and Human Services Cooperative Research Program, is supporting Epidemiological studies of possible health consequences of military service or employment in the Southwest Asia theater of operations during the Persian Gulf War. The USAMRMC is seeking proposals to study the incidence, prevalence, and nature of illnesses and symptoms and risk factors associated to symptoms or illnesses as a result of Persian Gulf War Service. No conventional Solicitation will be issued for this action, but the details of the requirements will appear in a future edition of the Commerce Business Daily (CBD). This action follows the guidelines of the RED streamlined contracting procedure outlined in DFARS 235.7000. Any offeror desiring to receive any amendments to this solicitation and any supplemental packages, if developed, should send their name and address, citing this announcement to the issuing office. U.S. ARMY MED.RESEARCH ACQUISITION ACT, MCMR-RMA, FORT DETRICK BLDG. 820, FREDERICK MD 21702-5014 (0125) -- Keith L. Cowing - Manager of Planning and Operations American Institute of Biological Sciences 10700 Parkridge Blvd Suite 380 - Reston, VA, USA 22091 703-758-1212 voice - 703-758-1222 fax kcowing@aibs.org - gopher://aibs.org From owner-diagnostics@net.bio.net Tue May 09 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!ahung From: ahung@ASRR.ARSUSDA.GOV Newsgroups: bionet.diagnostics Subject: Virus extraction Date: 10 May 1995 12:16:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues: I am about to extract more picorna-like viruses in honey bees, but would like to try different methods. Please give me references or your protocols for virus extraction using Freon. Thank you. Have a nice day! Akey :) From owner-diagnostics@net.bio.net Wed May 10 23:00:00 1995 Path: biosci!biosci!not-for-mail From: biotech@best.com Newsgroups: bionet.diagnostics Subject: in vitro diagnostics - antibodies Date: 11 May 1995 12:39:59 -0700 Organization: BEST Internet (415) 964-2378 Lines: 3 Sender: biohelp@net.bio.net Distribution: world Message-ID: <3otk1j$ma8@news1.best.com> Reply-To: biotech@best.com NNTP-Posting-Host: net.bio.net What is the regulatory status of using antibodies for clinical, in vitro diagnostics? I have heard rumors that the congress is about to change the regulations From owner-diagnostics@net.bio.net Wed May 10 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina Liu) Newsgroups: bionet.diagnostics Subject: Re: FDA Classification of antibodies Date: 11 May 1995 16:00:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 59 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Just thought you'd all be interested in this info. Thanks to Bryan Kiehl for the insight. >Return-Path: >Date: Wed, 10 May 1995 11:29:49 -0700 >To: jhughes@postbox.acs.ohio-state.edu (jhughes) >From: b3748@cts.com (Bryan Kiehl) >Subject: Re: FDA Classification of antibodies >Cc: biz-biotech@netcom.com >Sender: owner-biz-biotech@netcom.com >Precedence: list > >> Do you have any information about the FDA consideration to classify >>antibodies as Class II medical devices? Where is a good place to acquire >>information about Class I, II, III etc. medical devices? Any details you >>can provide would be greatly appreciated. >> >The classifications your mention are not for antibodies or chemical, but for >products distributed in the US. If the product is intended for clinical >uses, then the FDA is required to review claims of the product and assure >that it is "safe and effective" as claimed. There are three classes of >medical devices (devices include in vitro diagnostics). Class III is given >to products that have great affects on patients or patient management while >class I includes products with the least concern (e.g., throat swabs, >collection tubes, etc.). Recently, these classifications have been under >review and probably, at least for class I and II, don't have alot to do with >how a product is judged by the FDA. > >The laws providing this information are described in CFR's. How these are >interpreted are a matter of much debate and review. The antibodies the FDA >seems to be most concerned about are things like anti-CEA, AFP, and other >cancer markers. However, the law does not allow them to pick only some >reagents that are clinically used. > >I work in a diagnostic company that buys antibodies and puts them into kits. >Since we test these antibodies (quality assure) before using them in FDA >cleared kits, this is acceptable and the manufacturer of the antibody >doesn't need to be concerned. The problem arises when the original antibody >manufacturer sells these reagents to someone who uses them clinically >without qualifying them. The next obvious question is does testing (quality >assurance) in a laboratory qualify. The problem arises that no one reviews >the quality assurance testing done for this reagent by the laboratory. This >type of testing is not reviewed or understood by CLIA, the agency given the >mission to oversee labs. FDA has stated that they believe the FDA has the >authority and responsibility to oversee this. > >That's the problem. We all await the solution(s). > >Hope this helps. Please understand that much of the above is opinion. There >seems to be little in the way of facts. > > > > --- cliu@ic.net Internet connectivity provided by ICNET: +1 (313)998-0090 From owner-diagnostics@net.bio.net Thu May 11 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!news.clark.net!NewsWatcher!user From: kcowing@aibs.org (Keith L. Cowing) Newsgroups: bionet.biology.tropical,bionet.diagnostics Subject: UPDATE: US Army Persian Gulf War Illnesses Proposal Solicitation Date: Fri, 12 May 1995 17:21:42 -0500 Organization: American Institute of Biological Sciences Lines: 129 Message-ID: NNTP-Posting-Host: kcowing-ppp.clark.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit Xref: biosci bionet.biology.tropical:1000 bionet.diagnostics:113 The U.S. Army Medical Research and Materiel Command (USAMRMC) posted the following three announcements in the CBD (Commerce Business Daily) on 9 May 1995: Epidemiological Studies Persian Gulf War Illnesses (PGI-l) Pyridostigmine Bromide studies Persian Gulf War Illnesses (PGI-2) Clinical Research and Other Studies Persian Gulf War Illnesses (PGI-3) No conventional solicitation will be issued by the Army for this action. Updates will be posted to various newsgroups if/when they are received by AIBS. For updates on this and other biomedical RFPs, proposal solicitations, fellowships, and research opportunities, check the AIBS Gopher at gopher.aibs.org or via WWW at gopher://aibs.org - updates will appear in the PROFESSIONAL OPPORTUNITIES folder. NOTE: Direct all inquires to the US ARMY Point of Contact: CRAIG D. LEBO, 301-619-2036 U.S. ARMY MED. RESEARCH ACQUISITION ACT MCMR-RMA FORT DETRICK BLDG, 820, FREDERICK MD 21702-5014: +++++++++++++++++++++++++++++++++++++++++++++++ RECORD NUMBER: 1342PRA0008 CBD ISSUE DATE: 5/9/95 SECTION: Procurements FSC CODE: A TITLE: R&D STREAMLINED CONTRACT SOL DAMD17-95-#-0008 DUE 052495 Point of Contact: CRAIG D. LEBO, 301-619-2036 Epidemiological Studies Persian Gulf War Illnesses (PGI-l) The U.S. Army Medical Research and Materiel Command (USAMRMC) on behalf of the Departments of Defense, Veteran's Affairs and Health and Human Services Cooperative Research Program, is supporting Epidemiological studies of possible health consequences of military service or employment in the Southwest Asia theater of operations during the Persian Gulf War. The USAMRMC is seeking proposals to study the incidence, prevalence, and nature of illnesses and symptoms and risk factors associated to symptoms or illnesses as a result of Persian Gulf War Service. No conventional Solicitation will be issued for this action, but the details of the requirements will appear in a future edition of the Commerce Business Daily (CBD). This action follows the guidelines of the RED streamlined contracting procedure outlined in DFARS 235.7000. Any offeror desiring to receive any amendments to this solicitation and any supplemental packages, if developed, should send their name and address, citing this announcement to the issuing office. U.S. ARMY MED.RESEARCH ACQUISITION ACT, MCMR-RMA, FORT DETRICK BLDG. 820, FREDERICK MD 21702-5014 (0125) +++++++++++++++++++++++++++++++++++++++++++++++ NUMBER: 1342PRA0003 CBD ISSUE DATE; 5/9/95 SECTION: Procurements FSC CODE!: A TITLE: R&D STREAMLINED CONTRACT SOL DAMDl7-95-#-0009 DUE 05/24/95 , POC CRAIG D. LEBO, 301-619-2036 Pyridostigmine Bromide studies Persian Gulf War Illnesses (PGI-2) The U.S. Army Medical Research and Materiel. command (USAMRMC) on behalf of the Departments of Defense, Veteran's Affairs and Health and Human Services Cooperative Research Program, is supporting Pyridostigmine studies of possible health consequences of military service or employment in the Southwest Asia theater of operations during the Persian Gulf War. The USAMRMC is seeking proposals to determine whether the use of pyridostigmine bromide alone as a pretreatment against nerve agents, or combined with exposures to pesticides, other organophosphorous compound, caramates or other relevant chemicals would result in increased toxicity. No conventional solicitation will be issued for this actions but the details of the requirements will appear in a future edition of the Commerce Business Daily (CBD). This action follows the guidelines of the R&D streamlined contracting procedure outlined in DFARS 235.7000. Any offeror desiring to receive any amendments to this solicitation and any supplemental packages, if developed, should send their name and address, citing this announcement to the issuing office. U.S. ARMY MED. RESEARCH ACQUISITION ACT, MCMR-RMA (PGI-2), FORT DETRICK BLDG, 820, FREDERICK MD 21702-5014 +++++++++++++++++++++++++++++++++++++++++++++++ RECORD NUMBER: 1342PRA0006 CBD ISSUE DATE: 5/9/95 SECTION: Procurements FSC CODE: A TITLE: R&D STREAMLINED CONTRACT SOL DAMDl7-95-#-0010 DUE 05/24/95 POC CRAIG D. LEBO, 301-619-2036 Clinical Research and Other Studies Persian Gulf War Illnesses (PGI-3) The U.S. Army Medical Research and Materiel Command (USAMRMC) on behalf of the Departments, of Defense, Veteran's Affairs and Health and Human Services Cooperative Research Program, is supporting Clinical Research and other studies on the health consequences of military service or employment in the Southwest Asia theater of operations during the Persian Gulf War. The USAMRMC is seeking proposals for clinical studies and other research on the cause(s), mode(s), of transmission(s), and appropriate treatment( s) of Persian Gulf War illnesses.. No conventional solicitation will be is sued for this action, but the details of the requirements will appear in a future edition of the Commerce Business Daily (CBD). This action follows the guidelines of the R&D streamlined Contracting procedure outlined in DFARS 235.7000. Any offeror desiring to receive any amendments to this solicitation and any supplemental packages, if developer should send their name and address Citing this announcement to the issuing office. U.S. ARMY MED. RESEARCH ACQUISITION ACT, MCMR-RMA (PGI- 3) FORT DETRICK BLDG, 820 FREDERICK MD 21702-5014 -- Keith L. Cowing - Manager of Planning and Operations American Institute of Biological Sciences 10700 Parkridge Blvd Suite 380 - Reston, VA, USA 22091 703-758-1212 voice - 703-758-1222 fax kcowing@aibs.org - gopher://aibs.org From owner-diagnostics@net.bio.net Thu May 11 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!warp.cris.com!usenet From: jhpincus@cris.com (Jack H. Pincus) Newsgroups: bionet.diagnostics Subject: Re: in vitro diagnostics - antibodies Date: 12 May 1995 22:54:59 GMT Organization: Concentric Research Corporation Lines: 15 Distribution: world Message-ID: <3p0p03$kva@warp.cris.com> References: <3otk1j$ma8@news1.best.com> NNTP-Posting-Host: crc4.cris.com Mime-Version: 1.0 X-Newsreader: WinVN 0.99.3 In article <3otk1j$ma8@news1.best.com>, biotech@best.com says... > >What is the regulatory status of using antibodies for clinical, in >vitro diagnostics? I have heard rumors that the congress is about to >change the regulations I don't think it is Congress who is chaning the regulations. The FDA has proposed classifying certain antibodies used for histochemistry and cytochemistry as Class II medical devices. I don't know the details but some people feel that it could affect the development amd use of these antibodies. Jack H. Pincus jhpincus@cris.com From owner-diagnostics@net.bio.net Sat May 13 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!nntp.crl.com!decwrl!svc.portal.com!news1.best.com!usenet From: "B.B." Newsgroups: bionet.diagnostics Subject: SUPPORT FDA REFORM FOR IN VITRO DIAGS. READ THIS >> Date: 14 May 1995 01:23:49 GMT Organization: BEST Internet (415) 964-2378 Lines: 200 Message-ID: <3p3m35$e6k@news1.best.com> NNTP-Posting-Host: biotech.vip.best.com ON MAY 11, 1995 THE BLUE PRINT FOR FDA REFORM WILL BE INTRODUCED TO US CONGRESS REPRESENTATIVES SEE ANNOUNCEMENT BELOW. WHAT WOULD YOU GIVE TO SHAPE TOMORROW’S REGULATORY ENVIRONMENT? MDMA MEDICAL DEVICE MANUFACTURERS ASSOCIATION YOUR VOICE WILL BE HEARD ! GIVE THE MDMA 3 DAYS. . . of your time this coming May 11-13. With the new legislative environment, real reform is possible. Come be a part of it. Interact with leaders from the FDA, Capitol Hill, and Industry in an exchange of ideas about change. MEDICAL DEVICE MANUFACTURERS ASSOCIATION REGULATORY REFORM SUMMIT May 11-13, 1995 9 Washington, DC Ramada Plaza Hotel INVITEES FROM CAPITOL HILL: House Speaker Newt Gingrich Senator William Frist, MD Senator Orrin Hatch Senator Nancy Kassebaum Senator Rod Grams Congressman David McIntosh Congressman Thomas Bliley Congressman Jim Ramstad Congressman Joe Barton Congresswoman Anna Eschoo INVITEES FROM THE FDA: Dr. Burce Burlington, Director, CDRH Dr. Susan Alpert, Director, ODE John Stigi, Director, DSMA INVITED INDUSTRY SPEAKERS: Wayne Barlow, Wescor Stan Bauman, Immuno-Mycologics Thomas Duesterberg, Hudson Institute Steven Ferguson, Cook John Jennings Jeff Kimbell, MDMA Glen Lamey, Washington Legal Foundation Steven Levy, Threshold Technologies David Link, EXPERTech Associates Jeff Pierce, Citizens for a Sound Economy Ben Reid, Ben Reid & Associates David Shoultz, Varian & Associates Thomas Thompson, Quest Medical, Inc. Larry Pilot, McKenna & Cuneo THURSDAY, MAY 11 4:30pm-10:00pm "Regulatory and Legislative Reform Plan and Progress" "Congress and FDA: The Unique Relationship" "Success on Capitol Hill in 1995" FRIDAY, MAY 12 8:30am-6:00pm "Regulatory Reform Summit" "CDRH Accomplishments in '94 and a Plan for the Future" "Industry Overview" "History of 1976 Amendments and The Original Intent of 510(k) Exemption" "If You Could Change the CDRH Today, What Would You Do?" "Health Care Reform, Regulation, and Innovation in the Medical Device Industry" "The Reference List: What is it and Why Does it Exist?" "Capital Availability and Funding in the Current FDA Environment" "Tort Reform" "Use and Selection of Consultants" "Products Liability: How to Get it and What it Costs" Cocktail Reception SATURDAY, MAY 13 - 9:00am-1:00pm "Success on the Hill: What the MDMA and the Coalition Have Been Doing" "Update on FDA Oversight Hearings" "The Coalition's Legislative and Regulatory Reform of FDA/CDRH Concept" Closing Remarks Election of 1995 MDMA Board of Directors RESERVATION INFORMATION To make reservations for the Annual Membership Meeting and Regulatory Reform Summit, please mail, MEDICAL DEVICE MANUFACTURERS ASSOCIATION Attn: Marta Kennedy QUEST Medical, Inc. One Allentown Parkway / Allen, Texas 75002 Phone: 214-390-9800 ext 351 / Fax: 214-390- 2881 MDMA MEMBER: $250 NON MEMBER: $300 Payable by check, money order, or company purchase order number. To make hotel reservations, please contact: RAMADA PLAZA HOTEL Massachusetts & Vermont Avenues, N.W. Washington, DC 20005 Phone: 202-842-1300 Fax: 202-371-9602 National Medical Device Coalition's “BLUEPRINT FOR REFORM” The "Blueprint for Reform" began in Dallas, Texas in early December, 1994, when representatives of 10 trade associations met to discuss one common goal: FDA reform. The National Medical Device Coalition is working closely with House and Senate leaders to make these reforms a reality. This is the most important opportunity for FDA reform since the 1976 Amendments of the Food and Drug Administration which started the medical device approval process. Time is of the essence! Never again will there be an opportunity for our industry to gain control of its future. The final product is now ready and will be presented at the MDMA Regulatory Reform Summit in Washington, DC. Even if you are unable to attend the MDMA Regulatory Reform Summit, you can still pledge your support by giving $100 or more to the National Medical Device Coalition. In addition to the personal efforts of a number of the member associations, we plan to spend “major bucks" for this campaign and your commitment is critical. Please mail your check payable to: The National Medical Device Coalition 1575 Eye Street, N.W. Suite 500 Washington, DC 20005 The National Medical Device Coalition: American Electronics Association Association of Medical Diagnostics Manufacturers IVD Manufacturers Coalition Utah Biomedical Industry Council Indiana Medical Device Manufacturers Council Independent Reagent Manufacturers Association Medical Device Manufacturers Association Dental Implant Manufacturers Association Contact Lens Manufacturers Association Joint Commission on of Immunohistochemical Manufacturers MDMA MEDICAL DEVICE MANUFACTURERS ASSOCIATION WASHINGTON OFFICE: 202-898-5700 From owner-diagnostics@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!veths.no!Sewornu.Cudjoe From: Sewornu.Cudjoe@veths.no (Kofitsyo S. Cudjoe) Newsgroups: bionet.diagnostics Subject: Resquest for subscription Date: 15 May 1995 04:14:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505151113.EAA13559@net.bio.net> NNTP-Posting-Host: net.bio.net I would be grateful if the admnistrator of this discussion group could send me subscription forms and /or list me up as a subscriber. Thanks in advance, Dr. Kofitsyo S. Cudjoe From owner-diagnostics@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!rutgers!gatech!swrinde!cs.utexas.edu!not-for-mail From: brett_beitzel@msmtp.idde.saci.org (Brett Beitzel) Newsgroups: bionet.cellbiol,bionet.cellbiol.cytonet,bionet.diagnostics,bionet.molbio.methds-reagnts Subject: Human Tumor Cloning Assay Date: 15 May 1995 11:52:46 -0500 Organization: UTexas Mail-to-News Gateway Lines: 14 Sender: nobody@cs.utexas.edu Message-ID: NNTP-Posting-Host: news.cs.utexas.edu Xref: biosci bionet.cellbiol:2255 bionet.cellbiol.cytonet:217 bionet.diagnostics:117 bionet.molbio.methds-reagnts:28315 Subject:Human Tumor Cloning Assay Date: 5/15/95 Has anyone been successful in isolating tumor cell colonies from low mp agar (such as in the human tumor cloning assay) and growing them in a flask (T-25, etc.) Any help would be greatly appreciated. TIA, Brett Beitzel brett_beitzel@msmtp.idde.saci.org Cancer Therapy and Research Center San Antonio, TX From owner-diagnostics@net.bio.net Mon May 15 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!paladin.american.edu!newsfeed.ACO.net!swidir.switch.ch!univ-lyon1.fr!rockendo.univ-lyon1.fr!pcomm From: pcomm@cismibm.univ-lyon1.fr (Pierre Commercon) Newsgroups: bionet.diagnostics Subject: Wanted Kleihauer method Date: Tue, 16 May 1995 11:41:08 Organization: Universite Lyon1 Pharmacie Lines: 17 Message-ID: NNTP-Posting-Host: rockendo.univ-lyon1.fr X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hello HELP, HELP it s urgent I want use the Kleihauer method for a differential coloration of the foetal haematies. Boehringer stoped the production of this kit since two years. Can you give me a manual protocol for an equivalent of the Kleihauer method. I am looking for a technology with a coloration of the foetal haematies very easy to read. Merci Pierre From owner-diagnostics@net.bio.net Mon May 15 23:00:00 1995 Path: biosci!sial.com!oliverk From: oliverk@sial.com (Ken Oliver) Newsgroups: bionet.diagnostics Subject: diagnostics Date: 16 May 1995 05:11:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Please delete my subscription Ken Oliver _______________________________________________________________________ Ken Oliver | Sigma Diagnostics International Sales/Marketing | 545 South Ewing, St Louis, MO 63103 oliverk@sial.com | Fax: 314-534-3411 Tel: 314-771-5765 From owner-diagnostics@net.bio.net Wed May 17 23:00:00 1995 Path: biosci!OP1.UP.AC.ZA!KPAT1 From: KPAT1@OP1.UP.AC.ZA ("Fred Reyers") Newsgroups: bionet.diagnostics Subject: "isolation" of haemoglobin Date: 18 May 1995 06:51:21 -0700 Organization: University of Pretoria Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <137054566F9@op1.up.ac.za> NNTP-Posting-Host: net.bio.net Hi Diagnost Users, I have a small problem in "alchemy" I am planning a trial where I want to add varying concentrations of haemoglobin (hemoglobin for those West of the Atlantic) to fluids with predetermined concentrations of various chemical constiruents to evaluate what the effect of haemolysis would be on a range of analytical methods. In order to do this I need a fairly pure source of canine haemoglobin. I can wash erythrocytes (several times), lyse them and this gives me a solution of Hb, but it also contains many other erythrocyte metabolites. I can now precipitate the Hb (with TCA or Ammonium Sulphate), filter it and NOW comes my problem: How do I reconstitute the precipitated Hb (so that it at least resembles the Hb in haemolysed serum samples) from a TCA or Amm Sulphate precipitation? Can anyone out there help??? Thanks in anticipation of a flood of replies, Fred Fred Reyers (Prof & Head: Section of Clinical Pathology) Department of Medicine Faculty of Veterinary Science P.Bag X04, ONDERSTEPOORT, 0110, Rep. of South Africa Tel: 27-12-529 8288 FAX: 27-12-529 8308 or 27-12-344 2107 (Home) "The great tradgedies of science are the slaying of beautiful hypotheses by ugly facts" (Huxley) From owner-diagnostics@net.bio.net Thu May 18 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: "isolation" of haemoglobin Date: 19 May 1995 14:23:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <137054566F9@op1.up.ac.za> NNTP-Posting-Host: net.bio.net There should not be a problem resuspending hemoglobin from ammonium sulphate precipitate. Just macerate the precipitate with a glass rod and water dropwise, while continuing to macerate. At some point, the precipitate should dissolve into a clear solution. You will now that the hemoglobin is not denatured if it is still red (the binding of the heme group to the protein requires an intact molecule). If I am not mistaken, TCA will denature hemoglobin irreversibly. Falk Fish, Tel Aviv, Israel. From owner-diagnostics@net.bio.net Fri May 19 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!howland.reston.ans.net!nntp.crl.com!decwrl!svc.portal.com!news1.best.com!usenet From: "B.B." Newsgroups: bionet.diagnostics Subject: HELP TO REMOVE IN VITRO DIAGNOSTICS FROM FDA >>>> Date: 20 May 1995 03:10:30 GMT Organization: BEST Internet (415) 964-2378 Lines: 62 Message-ID: <3pjmj6$r1a@news1.best.com> NNTP-Posting-Host: biotech.vip.best.com REPORT ON MDMA’S SUMMIT FOR FDA REFORM On May 11-13, 1995 the Medical Devices Manufacturers Association, MDMA, held the SUMMIT FOR FDA REFORM in Washington DC. A “ Blueprint for FDA Reform”, advanced by MDMA, was discussed at the meeting. During the celebration of the SUMMIT, over 70 congress representatives were approached by members of MDMA who officially delivered the Blueprint for FDA Reform, several representatives offered to translate the blueprint into legislative language and introduce it to Congress. The speaker of the House, Newt Ginrich asserted that the House will pass FDA reform containing most of the issue outlined in the Blueprint. It appears that FDA REFORM will continue to be a hot issue at Capitol Hill. The SUMMIT For FDA Reform is supported by the following organizations, representing 700 companies and constituting The National Medical Device Coalition:. · American Electronics Association · Association of Medical Diagnostics Manufacturers · IVD Manufacturers Coalition · Utah Biomedical Industry Council · Indiana Medical Device Manufacturers Council · Independent Reagent Manufacturers Association · Medical Device Manufacturers Association · Dental Implant Manufacturers Association · Contact Lens Manufacturers Association · Joint Council of Immunohistochemical Manufacturers The in vitro diagnostic industry should not continue to be regulated with the same measure used to control drugs. There is a clear and profound difference between pharmaceuticals and in vitro diagnostics (IVD). The in vitro diagnostic industry, integrated in its majority by small companies, has been placed under the FDA agency for too long, in detriment of both health care and manufacturers. The present regulatory arrangement is unfit for our industry and tends to hamper the implementation of new technologies in health care. We would like to encourage all of those who believe it is time to separate in vitro diagnostics from under the FDA’s jurisdiction, to help by supporting this movement. It is important for you to voice your opinion in support of immediate changes of the FDA. This action must take place NOW. Contact your District’s House Representatives and Senators, introduce them to the Blueprint for FDA Reform and request they support FDA reform including removal of in vitro diagnostics from FDA. Copies of the MDMA blueprint For FDA Reform can be obtained directly from NMDC The National Medical Device Coalition 1575 Eye Street, N.W. Suite 500 Washington, DC 20005 Phone: 202-898-5700 From owner-diagnostics@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!CIX.COMPULINK.CO.UK!knorrie From: knorrie@CIX.COMPULINK.CO.UK (Kenneth Norrie) Newsgroups: bionet.diagnostics Subject: Help please Date: 22 May 1995 10:56:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: knorrie@cix.compulink.co.uk NNTP-Posting-Host: net.bio.net Help I'm looking for sources of plate ELISA test software and have been told that this (a mailing-list?) is worth a look. What do I have to do to find out more? Kenneth Norrie Tel: +44 1506 416993 Level One Software Limited Email: knorrie@cix.compulink.co.uk Livingston, Scotland Mobile: 0850 424705 From owner-diagnostics@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU Newsgroups: bionet.diagnostics Subject: postdoc Date: 22 May 1995 13:13:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <950522052659.20200704@LIFSCI.SDSU.EDU> NNTP-Posting-Host: net.bio.net PLEASE POST Postdoctoral Fellowship Salmonella genome May 22nd 1995 Dear colleague, The primary postdoc on our Salmonella project has been offered his own laboratory at DOE/WSU. I am thus looking for someone to take over his position as soon as possible. If you know someone who might be interested I would be grateful if you could direct that person to me by e-mail to McClelland@lifsci.sdsu.edu or by FAX to 619 535 5472. The first step of this project is well advanced with 95% of the genome covered by an order and overlapping set of lambda clones. Approximately, 20% of these have been restriction mapped. We have delineated in these clones many of the known loops that distinguish Salmonella from E. coli. The most interesting part of the project is just starting in which we delineate the role of some of these lifestyle-specific regions. The next stage of the project will also involve the use of RNA fingerprinting by arbitiarily primed PCR in identify coordinately regulated genes in point mutations and large deletions in these regions (Wong K.K. and McClelland M. 1994. A stress-induced gene from Salmonella typhimurium identified by arbitrarily primed PCR of RNA (RAP). Proc. Natl. Acad. Sci. U.S.A. 91:639- 643; McClelland et al., Trends in Genetics, June 1995). Se also Wong K.K. and McClelland M. 1994. High-resolution restriction map for a 240- kilobase region spanning 91- to 96 minutes on the Salmonella typhimurium LT2 genome. J. Bacteriol. 176: 5729-5734. I am seeking someone with leadership and organizational skills who will coordinate with our collaborator Ken Sanderson and will direct a technician on the project. I would greatly appreicate your help in locating such an individual. Michael McClelland Director, Cal. Inst. Biol. Research 11099 North Torrey Pines Road La Jolla CA 92037 Phone 619 535 5486 FAX 619 535 5472 E-mail McClelland@lifsci.sdsu.edu From owner-diagnostics@net.bio.net Tue May 23 23:00:00 1995 Path: biosci!COMPUSERVE.COM!100416.735 From: 100416.735@COMPUSERVE.COM (Diagnostics Club) Newsgroups: bionet.diagnostics Subject: Face to Face Mtg Date: 24 May 1995 06:46:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 76 Sender: daemon@net.bio.net Distribution: world Message-ID: <950524133810_100416.735_BHG134-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net DIAGNOSTICS TRANSFER FOR BUSINESS A Diagnostics Club meeting aimed at stimulating the dialogue between research organizations and commercial organizations. The Objective: The aim of this meeting is to encourage collaborative partnerships between diagnostic companies and research organizations. Rather than general discussions, the event will be structured around individual pre-arranged meetings between organizations who have expressed an interest in talking to each other. During the course of the event, the participating organizations can expect to have up to 16 scheduled meetings, of half-an-hour duration. At the end of the event you should have: 1) Identified potential partners 2) Identified opportunities for collaboration. Why should your organization participate? Synergistic partnerships between companies and researchers is the goal that many organizations seek. This meeting offers you opportunity to find partners in diagnostics in the medical, agri-food, environmental and veterinary sectors. So, if you are interested in funding your research or extending your business, this is a time efficient, cost-effective and focussed way to develop partnerships. Venue: The meeting will take place in rooms adjacent to the exhibition at Earls Court 2, London, UK, during the Science in Action Exhibition on the 4th October 1995 How will the event work? Step 1: "Seller" Registration Form 1) Please complete a registration form . Two attendees can be given, but the first one will be our contact with your organization. 2) Present your organization and its research activities. 3) Outline the organization's area of business, particular specialities, and know how. 4) Describe in detail (circa 100 words) and indicate by ticking the appropriate box the stage of development of the project(s) for which collaboration is sought. Step 2: The Catalogue of Technology for Transfer The information from the "seller" respondents will be compiled into a catalogue and mailed to pre-selected and targeted potential commercial collaborators by mid July 1995. This will enable them to select the research organizations they wish to meet for discussions. Step 3: Your Meeting Schedule A meeting schedule will be organised by matching the requirements expressed by each participant. An individual meeting schedule will be drawn up for each organization-delegate. This will give details of the time and place of each meeting along with the name and full details of each organization you will be meeting. Step 4: The Meeting The meeting will be held in rooms adjacent to the Exhibition. There will be upwards of 20 tables - one for each 'seller'. Starting at 10.00 on the 4th November the first set of twenty discussions will take place. Subsequently at half-hour intervals a bell will sound and the "buyer participants" move to the next on their schedule of chosen "sellers" . The half-hour meetings changeovers must be, and will be rigorously applied. To facilitate maximum discussion time a buffet and 'continuous coffee' will be provided. Step 5: Confidentiality Pro-forma confidentiality agreements will be available. Step 6: Timetable Cut-off date for return of "seller" registration:- 1st July Opportunities out (to "buyers") mid July Appointment bookings by end of August Appointment schedules two weeks before meeting. For further information (including registration fee and registration forms), please contact: Dr Valerie Owen, Diagnostics Club Manager on tel: +44 1908 647417, fax: +44 1908 271612, email: CompuServe 100416,735 (Internet:100416.735@compuserve.com). From owner-diagnostics@net.bio.net Wed May 24 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!sunsite.doc.ic.ac.uk!doc.news.pipex.net!pipex!demon!btnet!news.compulink.co.uk!cix.compulink.co.uk!usenet From: knorrie@cix.compulink.co.uk ("Kenneth Norrie") Subject: Plate ELISA data handling software Message-ID: Organization: Compulink Information eXchange Date: Thu, 25 May 1995 19:32:48 GMT X-News-Software: Ameol Lines: 36 -- The following is a modified version of a previous post -- -- on bionet.virology and bionet.software -- I am looking for sources of microplate ELISA data handling software, on behalf of a client whose application is mainly virus detection. We actually have a fairly detailed set of requirements, but for now all I am looking for is the contact information for any suitable suppliers. Whether it runs on a PC, MAC or whatever, is a basic program or a fully automated hardware/software system is not important at this stage. So far the names I have are the following: Dynatech Labs, DIAS Biotek Instruments, Omni-Series ASYS Hitech, DigiScan Bio-Rad Labs, Microplate Manager Biosoft, AssayZap BioMetallics, DeltaSoft Molecular Devices, SoftMax Leading Edge Research, ELISA! Please let me know if you know of others. I'd also like to know if there are other suitable newsgroups to post this in, and (as a non-biologist) I'd appreciate being pointed at any technical publications which may run advertisements for such equipment. Also, if anybody out there would like to share their experiences of using ELISA software (good or bad) I would very much like to hear from them. Kenneth Norrie Tel: +44 1506 416993 Level One Software Limited Email: knorrie@cix.compulink.co.uk Livingston, Scotland Mobile: 0850 424705 From owner-diagnostics@net.bio.net Wed May 24 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!sunsite.doc.ic.ac.uk!doc.news.pipex.net!pipex!demon!btnet!news.compulink.co.uk!cix.compulink.co.uk!usenet From: knorrie@cix.compulink.co.uk ("Kenneth Norrie") Subject: Re: Plate ELISA data handling software Message-ID: Organization: Compulink Information eXchange References: Date: Thu, 25 May 1995 20:49:39 GMT X-News-Software: Ameol Lines: 15 I have discovered that the current (or very recent) issue of a US technical periodical called 'American Biotechnology Laboratory' includes a 1995 Buyers' Guide, which lists several suppliers of ELISA software and systems. I would very much like to get hold of this guide, but have been unable to find anyone over here who has even heard of it, let alone has a copy. So, is there a kind soul over the water who could send this to me? I will of course reimburse post + packing etc. Please contact me by e-mail if you can do this! Kenneth Norrie Tel: +44 1506 416993 Level One Software Limited Email: knorrie@cix.compulink.co.uk Livingston, Scotland Mobile: 0850 424705 From owner-diagnostics@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!mbdj From: mbdj@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Re: Plate ELISA data handling software Date: 26 May 1995 01:15:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505260813.JAA22367@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net Assay Composer" It looks quite impressive high throughput, versatile etc, but it also looks very expensive. I seem to remember beckman having an automated system as well, but that like much of the mail I get seems to have found it's way to the bin. Dave. From owner-diagnostics@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!isn.net!howatt From: howatt@isn.net ("Steve Howatt") Newsgroups: bionet.diagnostics Subject: Re: Plate ELISA data handling software Date: 25 May 1995 18:45:00 -0700 Organization: Island Services Network Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505260037.VAA08034@phoenix.isn.net> NNTP-Posting-Host: net.bio.net I would very much like to recieve the following information as well. Thank you in advance... > To: diagnost@net.bio.net > From: knorrie@cix.compulink.co.uk ("Kenneth Norrie") > Subject: Re: Plate ELISA data handling software > Date: Thu, 25 May 1995 20:49:39 GMT > I have discovered that the current (or very recent) issue of a US > technical periodical called 'American Biotechnology Laboratory' includes > a 1995 Buyers' Guide, which lists several suppliers of ELISA software and > systems. I would very much like to get hold of this guide, but have been > unable to find anyone over here who has even heard of it, let alone has a > copy. > > So, is there a kind soul over the water who could send this to me? I will > of course reimburse post + packing etc. Please contact me by e-mail if > you can do this! > > > Kenneth Norrie Tel: +44 1506 416993 > Level One Software Limited Email: knorrie@cix.compulink.co.uk > Livingston, Scotland Mobile: 0850 424705 > > Steve Howatt, P.Ag. email address: howatt@isn.net ************************************************************** Atlantic AgriTech - Agricultural Research and Consulting - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - MicroProp PEI - Potato Tissue Culture and Minituber Production ************************************************************** Prince Edward Island, CANADA From owner-diagnostics@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Plate ELISA data handling software Date: 26 May 1995 01:41:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net You should try also SLT instruments in Austria. They have a reliable ELISA reader with built-in software + plug-in software modules with varying degrees of complexicity. Falk Fish, Tel-Aviv, Israel. From owner-diagnostics@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!REV.RE.UOKHSC.EDU!f755 From: f755@REV.RE.UOKHSC.EDU Newsgroups: bionet.diagnostics Subject: FDA REFORM Date: 26 May 1995 08:13:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <95052610083188@zena.uokhsc.edu> NNTP-Posting-Host: net.bio.net The list of manufacturers lobbying for FDA reform is impressive and understandable, particularly if there is a profit motive behind the "reform". I am not impressed. Why should the scientific community support your reforms? And, by the way, just what reforms are you proposing? From owner-diagnostics@net.bio.net Fri May 26 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!nntp.crl.com!decwrl!svc.portal.com!news1.best.com!usenet From: "B.B." Newsgroups: bionet.diagnostics Subject: Re: FDA REFORM Date: 27 May 1995 02:35:22 GMT Organization: BEST Internet (415) 964-2378 Lines: 24 Message-ID: <3q635a$9vn@news1.best.com> References: <95052610083188@zena.uokhsc.edu> NNTP-Posting-Host: biotech.vip.best.com f755@REV.RE.UOKHSC.EDU wrote: > Seems like you are against this FDA reform even if you still have not seem the proposal. You have answered before you heard the question. If you would like to know more about the FDA reform, call the MDMA telephone number listed in the posting and request a copy of their reform blueprint.For even more on the FDA reform, look at proposals of HIMA (Health Industry Manufacturers Association), the largest heath-related association with a representation of 90 % of the industry, equivalent to 36 billion Dollars market share. I respect your "apriori" opposition to unknown arguments but it is not an inspiring virtue in my book. I prefer to follow a different order in my reasonings. Best regards, B.B. > From owner-diagnostics@net.bio.net Sat May 27 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!connix.com!pkramer.connix.com!user From: pkramer@connix.com (Peter Kramer) Newsgroups: bionet.diagnostics Subject: HIV screening "window" & PCR Date: Sun, 28 May 1995 08:11:16 -0500 Organization: Connix - The Connecticut Internet Exchange Lines: 13 Message-ID: NNTP-Posting-Host: pkramer.connix.com I've read some recent literature that suggests that the time between HIV infection and detection (i.e. the window) for blood screening can be substantially reduced by including PCR testing in addition to the standard ELIZA screening. Can anyone give me an idea what the "window' size is for some of the PCR methods that are currently available? Thanks. Peter Kramer pkramer@connix.com From owner-diagnostics@net.bio.net Sat May 27 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bloom-beacon.mit.edu!usc!crash!kiehl From: b3748@cts.com (Bryan Kiehl) Subject: Centromere Antigen Organization: CTSNET Date: Sun, 28 May 1995 00:05:38 GMT Message-ID: <3q8eoi$8rs_001@kiehl.cts.com> X-Newsreader: News Xpress Version 1.0 Beta #3 Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: kiehl.cts.com Lines: 2 I work in a commercial diagnostic company and would like to make an assay to detect human anti-centromere. Are there any recombinant antigens available? From owner-diagnostics@net.bio.net Sun May 28 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: HIV screening "window" & PCR Date: 29 May 1995 14:20:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net The current window's median is about 6 months. Theoretically, PCR should reduce the window, however, the actual performance does not always live to its expectations. The problem may emanate from: 1. Specimen preparation problems; 2. The virus is not located in circulating periferal lymphocytes at the early stages of the infection. Therefore, even very efficient amplification methods are unable to detect. The are some groups working on other methods for window shrinking with some impressive results. You should realize also that the window is an estimate: it is difficult, if not impossible, to know when the active infection started. Falk Fish From owner-diagnostics@net.bio.net Sun May 28 23:00:00 1995 Path: biosci!MAIL.CIP.ORG.EC!vantslot From: vantslot@MAIL.CIP.ORG.EC (Andre van 't Slot) Newsgroups: bionet.diagnostics Subject: PCR at 3000 masl Date: 29 May 1995 09:17:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <9505291035.AA02942@mail.cip.org.ec> NNTP-Posting-Host: net.bio.net Finally the solution to our PCR problem. It has been a long time since we received the last suggestions for this problem and we hope that you can still remember our questions about PCR at 3050 meters above sea level. Water boils at 89 C and we were afraid that we would not be able to heat up the tubes to 94 C. We are very grateful to all of you who thought about it and gave us suggestions. Fortunately, the capped tubes consisting sample and mineral oil did not pop open and reached 94 C without problem. So far we have not been able to find out if the high altitude causes other problems. With friendly greetings, Greg, Andre and Mariu. CIP-Quito. -- Andre van 't Slot Centro Internacional de la Papa Casilla 17-16-129-CEQ Quito, Ecuador Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec -- Andre van 't Slot Centro Internacional de la Papa Casilla 17-16-129-CEQ Quito, Ecuador Telf. +593-2-690990, Internet: VANTSLOT@cip.org.ec From owner-diagnostics@net.bio.net Tue May 30 23:00:00 1995 Path: biosci!IC.NET!cliu From: cliu@IC.NET (Christina Liu) Newsgroups: bionet.diagnostics Subject: Re: Plate ELISA data handling software Date: 31 May 1995 08:06:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 84 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >I have discovered that the current (or very recent) issue of a US >technical periodical called 'American Biotechnology Laboratory' includes >a 1995 Buyers' Guide, which lists several suppliers of ELISA software and >systems. I would very much like to get hold of this guide, but have been >unable to find anyone over here who has even heard of it, let alone has a >copy. > >So, is there a kind soul over the water who could send this to me? I will >of course reimburse post + packing etc. Please contact me by e-mail if >you can do this! > > >Kenneth Norrie Tel: +44 1506 416993 >Level One Software Limited Email: knorrie@cix.compulink.co.uk >Livingston, Scotland Mobile: 0850 424705 > Kenneth: I have a copy of the ABL Buyers' Guide. You should be able to get a copy of your own by calling International Scientific Communications, Inc., 30 Controls Drive, P.,O. Box 870, Shelton, CT 06484-0870 U.S.A., Phone (203) 926-9300. They charge $25.00 (U.S.) per copy to nonsubscribers, and also publish other trade magazines such as European Biotechnology Laboratory and American Clinical Laboratory, I believe. Regarding your specific question regarding ELISA readers, I looked up the section on ELISA readers and found the following companies listed (although they did not specify which ones included their own software). I hope this helps! AAB, Advanced American Biotechnology American Bioanalytical Beckman Instruments, Inc. Bio-Rad Laboratories Diagnostic Group Bio-Rad Laboratories, (UK) Ltd. Bio-Rad Laboratories, Life Science Group Bio-Tek Instruments, Inc. BioDesign Inc. of New York BioWhittaker, Inc. Brendan Scientific Buhlmann Laboratories AG Cambridge Technology, Inc. (617) 923-1181 Denley Instruments, Inc.; Denley Instruments, Ltd. Dynatech Laboratories, Inc. (703) 631-7800 Dynatech Laboratories, Ltd. Eurogenetics Genzomed Biotech, Inc. IDS Ltd. INCSTAR Corp. INOVA Diagnostics, Inc. International pbi S.P.a. Kem-En-Tec A/S Labsystems (UK) Ltd. Labsystems (617) 449-8060 MEDGENIX Diagnostics Molecular Devices Corporation (415) 322-4700 Ohmicron Environmental Diagnostics Quatro Biosystems Ltd. Rosys Inc. SCINICS Corp. Sclavo Inc. Serono-Baker Diagnostics, Inc. SLT Labinstruments SLT Labinstruments GmbH Tecan AG Transia Ultra-Lum, Inc. (310)324-2247 VWR Scientific Zinsser Analytic GmbH I hope this helps! Christina Note: I am not an employee of any of the above companies nor am I an employee of International Scientific Communications, and also do not reflect any interests of my employer. --- cliu@ic.net Internet connectivity provided by ICNET: +1 (313)998-0090 From owner-diagnostics@net.bio.net Wed May 31 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!swrinde!gatech!bloom-beacon.mit.edu!world!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!news.ucalgary.ca!acs4.acs.ucalgary.ca!sjszarka From: Steven Joseph Szarka Newsgroups: bionet.diagnostics Subject: Help Needed Understanding Allergy ELISA Results Date: Thu, 1 Jun 1995 12:22:15 -0600 Organization: The University of Calgary Lines: 52 Message-ID: NNTP-Posting-Host: acs4.acs.ucalgary.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Several members of my family have had ELISA tests done to screen for blood IgG4 and IgE against a list of potential food allergens. The results were presented in tabular form with a numerical values attached to each food sample. The results are then divided into 4 columns depending on the numerical rating: 1. essentially no detection 2. low (10-50) 3. intermediate (50-300) 4. High (300-3000) To help understand what foods (all, certain, or none) should be avoided I have the following questions: 1. Does any amount of detectable IgE indicate a food allergy or is there a cut-off (eg. only those in the high column)? If there is a cut-off; is it arbitrary or physiologically based? 2. I assume the RAST (Radioactive equivalent?) is more sensitive; is this higher sensitivity necessary to identify all allergies? 3. Would continual exposure to a low reactive food item increase the IgE signal so that eventually it moves into the 'high' category and becomes a problem (assuming the 'low' foods are presently not a problem)? 4. Based on the experience of people who perform these tests; what is the ELISA pattern generally seen in the population? Do most people show: i. no reaction ii. low reaction to many items iii. a high reaction to a few items combined with either i. or ii. 5. Is the response to question 4 different for children compared to adults? Thanks to all those who can offer information Steve _______________________________________________________________________ Steven Szarka Botany Division Dept. of Biological Sciences Email: sjszarka@acs.ucalgary.ca University of Calgary FAX: (403) 289-9311 2500 University Drive NW Phone: (403) 220-7907 Calgary, AB, Canada T2N 1N4 _______________________________________________________________________ From owner-diagnostics@net.bio.net Wed May 31 23:00:00 1995 Path: biosci!ZEUS.DATASRV.CO.IL!ila2027 From: ila2027@ZEUS.DATASRV.CO.IL (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Help Needed Understanding Allergy ELISA Results Date: 1 Jun 1995 13:44:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Food allergy is a sticky issues: most doctors do not believe that it exists (except for milk, peanuts and seafood) but laternative medicine patriots and some opposition doctors do believe in its wide array of symptoms. First, ask the lab, which conducted the tests to provide you with an analysis and interpretation. If it is a serious establishment, specializing in food allergy, they would employ a dietician, who will develop an appropriate diet program based on the findings. It is difficult to say when the presence of IgE or IgG4 reflects an on going pathology. The definitive test for allergy is the "Elimination Diet", where the suspected foods are eliminated for long periods and the symptoms are closely monitored. After elimination comes the challenge with close follow-up of the symptoms. You should know what are your family's symptoms and what is the reason for conducting those tests to begin with. These will direct you in monitoring elimination and challenge. The dietician is very important since many modern commercial foods are of complex origin, so that sometimes you will have to eliminate more foods than you would have expected, in order to avoid a components. Thus, breads contain milk products, processed fish (e.g. fish fingers) contain shellfish and other critters and hamburgers contain soybean god-knows-what. As to low level antibody: the food allerfy advocate do indeed warn that allergic reactions may worsen if one is continuously subjecting one's digestive system to this food. Therefore, try to separate the intake of such foods by long intervals. It is generally a good idea to enrich the components of your diet and eat different foods on different days (e.g. you do not have to go to MacDonald's every day: do not forget Burger King, Hardee's and Jack in the Box). By the way, which lab did those tests? I hope the above gives you some idea how to proceed, although I did not answer all the questions and certainly not in any order. Falk Fish, Tel-Aviv, Israel