From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!BLUE.WEEG.UIOWA.EDU!twiese From: twiese@BLUE.WEEG.UIOWA.EDU ("T. Wiese") Newsgroups: bionet.diagnostics Subject: Source of bulk carbohydrates Date: 1 Aug 1995 18:59:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues, Does anyone know of sources of bulk quantities of carbohydrates (kg amounts) other than Pfanstiel? Does Waco (sp?) of Japan isolate carbohydrates in bulk? Thank you in advance, Thomas Wiese From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!MAIL.FWS.GOV!Mail-9DE~SMTP From: Mail-9DE~SMTP@MAIL.FWS.GOV Newsgroups: bionet.diagnostics Subject: plant viruses Date: 2 Aug 1995 08:47:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507028073.AA807369263@mail.fws.gov> NNTP-Posting-Host: net.bio.net User coler is not defined Original text follows ---------------------------------------------- Received: from net.bio.net by mail.fws.gov (SMTPLINK V2.10.03) X-Envelope-From: BIOSCI-REQUEST@net.bio.net Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA29333 for diagnost-list; Wed, 2 Aug 1995 04:32:50 -0700 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id EAA29329 for diagnost-arpanet; Wed, 2 Aug 1995 04:32:49 -0700 To: diagnost@net.bio.net From: burns@sasa.gov.uk (Robert Burns) Subject: Potato Virus V Date: 2 Aug 1995 04:32:44 -0700 Sender: daemon@net.bio.net Message-ID: NNTP-Posting-Host: net.bio.net Hi, any plant virologists out there? We are looking for isolates of PVV for evaluation of some Mabs that we have, can anyone help? We are particularly interested in sources outside of UK/Europe Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!MAIL.FWS.GOV!Mail-9DE~SMTP From: Mail-9DE~SMTP@MAIL.FWS.GOV Newsgroups: bionet.diagnostics Subject: conjugate stability Date: 2 Aug 1995 08:44:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507028073.AA807368724@mail.fws.gov> NNTP-Posting-Host: net.bio.net User coler is not defined Received: from net.bio.net by mail.fws.gov (SMTPLINK V2.10.03) X-Envelope-From: BIOSCI-REQUEST@net.bio.net Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA29200 for diagnost-list; Wed, 2 Aug 1995 04:29:59 -0700 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id EAA29192 for diagnost-arpanet; Wed, 2 Aug 1995 04:29:56 -0700 To: diagnost@net.bio.net From: burns@sasa.gov.uk (Robert Burns) Subject: storage of conjugates Date: 2 Aug 1995 04:29:45 -0700 Sender: daemon@net.bio.net Message-ID: NNTP-Posting-Host: net.bio.net We routinely make monoclonal AP conjugates by thiolation of the antibody followed by reaction with activated AP. We find that thge storage conditions for each conjugate is dependent on the Mab used. Some conjugates store well at 4C, some don't. All of them store well at -20C. We have also been looking at a Pierce product called superfreeze which allows liquid storage of conjugates at -20C which should increase longevity. We find that some conjugates store better with it and some without. BTW in some cases conjugates stored with superfreeze had decreased activity compared to aliquots of the same batch stored at 4C without it! I think with Mab conjugates it all depends on the parent antibody. Anybody else got any conjugate stories? Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test ignore Date: 2 Aug 1995 08:40:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: Potato Virus V Date: 2 Aug 1995 04:32:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi, any plant virologists out there? We are looking for isolates of PVV for evaluation of some Mabs that we have, can anyone help? We are particularly interested in sources outside of UK/Europe Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: storage of conjugates Date: 2 Aug 1995 04:29:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net We routinely make monoclonal AP conjugates by thiolation of the antibody followed by reaction with activated AP. We find that thge storage conditions for each conjugate is dependent on the Mab used. Some conjugates store well at 4C, some don't. All of them store well at -20C. We have also been looking at a Pierce product called superfreeze which allows liquid storage of conjugates at -20C which should increase longevity. We find that some conjugates store better with it and some without. BTW in some cases conjugates stored with superfreeze had decreased activity compared to aliquots of the same batch stored at 4C without it! I think with Mab conjugates it all depends on the parent antibody. Anybody else got any conjugate stories? Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!howland.reston.ans.net!news.sprintlink.net!uunet!in1.uu.net!uunet.ca!news.uunet.ca!inforamp.net!usenet From: rleavitt@on.novamann.ca (Randy Leavitt) Newsgroups: bionet.diagnostics Subject: Re: Single substrate component for TMB Date: Tue, 01 Aug 95 13:10:25 PST Organization: GfWare Lines: 24 Distribution: world Message-ID: <3vlmsj$97@inforamp.net> References: <199507161426.WAA27105@einstein.technet.sg> NNTP-Posting-Host: ts2-02.inforamp.net Mime-Version: 1.0 X-Newsreader: WinVN 0.93.6 In article <199507161426.WAA27105@einstein.technet.sg>, pohyam@TECHNET.SG says... > >I have always been using the two component OPD and BCIP/NBT coupled with HRP >for my ELISA assays. Recently, I read that a new single component TMB >substrate for HRP is on sale. Has anyone tried this? Is it superior in terms >of OD duplicity and stability of colour development? Can someone share their >experience with me? > > > We have used the single component TMB substrate for the past 2 years in our single step ELISAs for drugs in the race horse. Use of the product (K-Blue) has improved sensitivities, precision and made for easier handling than the classical 2 component system. K-Blue is available from ELISA TECHNOLOGIES in Lexington, KY (606-266-9556). From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm!cs.utexas.edu!swrinde!tank.news.pipex.net!pipex!news.sprintlink.net!crash!kiehl From: b3748@cts.com (Bryan Kiehl) Subject: Re: Storage of conjugates Organization: CTSNET Date: Wed, 2 Aug 1995 01:34:03 GMT Message-ID: <3vmkmb$1s4k_001@kiehl.cts.com> X-Newsreader: News Xpress Version 1.0 Beta #3 References: <199508010812.BAA11589@net.bio.net> Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: kiehl.cts.com Lines: 19 In article <199508010812.BAA11589@net.bio.net>, vigfrid.ness@veths.no (Vigfrid Ness) wrote: >Hi >Can anybody tell me the best way to store conjugated antibodies???? > >Viffi Ness >DYNAL AS > Conjugates (alkaline phosphatase or peroxidase), assuming they were prepared properly, can be stored at refrigeration temperatures for many months and are stable frozen for years. In most cases, the reason for eventual inactivity is microbial contamination. Bryan Kiehl GenBio 15222-A Avenue of Science San Diego, CA 92128 (619) 592-9300, ext 309 (619) 592-9400 [FAX] From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!agresearch.cri.nz!HartA From: HartA@agresearch.cri.nz Newsgroups: bionet.diagnostics Subject: Nitric oxide Date: 1 Aug 1995 22:08:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HTLSLPMQJQ002DY7@INVERMAY.CRI.NZ> NNTP-Posting-Host: net.bio.net Dear Newsgroup members, Does anyone have details on a reliable method for measuring atmospheric nitric oxide (NO). Cheers, Alan Hart From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!biosci!not-for-mail From: Simon Eaton Newsgroups: bionet.diagnostics,bionet.metabolic-reg,bionet.announce,bionet.general,sci.med Subject: 2nd Shire Workshop: Screening for Inborn Errors of Metabolism Date: 2 Aug 1995 10:38:42 -0700 Organization: Newcastle University, UK Lines: 39 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <3vns2c$atp@whitbeck.ncl.ac.uk> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.diagnostics:234 bionet.metabolic-reg:513 bionet.announce:2359 bionet.general:16384 sci.med:87476 2nd SHIRE WORKSHOP "Screening for Inborn Errors of Metabolism" Current Status and Future Developments 25th September 1995 Lecture Theatre The Sir James Spence Institute of Child Health The Royal Victoria Infirmary Newcastle upon Tyne NE1 4LP PROGRAMME 2.00 - 2.10pm Introduction and welcome Professor A W Craft 2.10 - 2.40pm Diagnosis of lysosomal storage disorders Dr GTN Besley, Willink Biochemical Genetics Unit, Royal Manchester Children's Hospital 2.40 - 3.20pm Neonatal Screening - what do we screen for and why? Dr RJ Pollit Neonatal Screening Laboratory The Children's Hospital, Sheffield 3.20 - 3.40pm Tea/Coffee 3.40 - 4.20pm Neonatal Screening - how well do we screen? Dr A Galloway Neonatal Screening Laboratory, Dryburn Hospital 4.20 - 5.00pm Neonatal Screening - what could we screen for and how? Professor K Bartlett Department of Child Health ___________________________________________________________________________ Enquiries to: Kim.Bartlett@ncl.ac.uk or Anne.Kennedy@ncl.ac.uk ___________________________________________________________________________ From owner-diagnostics@net.bio.net Tue Aug 01 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: plant viruses (additional) Date: 2 Aug 1995 09:52:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Whoops, should have defined PVV in my previous post. It is Potato Virus V Thanks Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Wed Aug 02 23:00:00 1995 Path: biosci!MAIL.FWS.GOV!Mail-9DE~SMTP From: Mail-9DE~SMTP@MAIL.FWS.GOV Newsgroups: bionet.diagnostics Subject: plant viruses (additional) Date: 3 Aug 1995 02:52:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507028073.AA807388860@mail.fws.gov> NNTP-Posting-Host: net.bio.net ---------------------------------------------- Received: from net.bio.net by mail.fws.gov (SMTPLINK V2.10.03) X-Envelope-From: BIOSCI-REQUEST@net.bio.net Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id JAA14977 for diagnost-list; Wed, 2 Aug 1995 09:53:02 -0700 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id JAA14968 for diagnost-arpanet; Wed, 2 Aug 1995 09:52:59 -0700 To: diagnost@net.bio.net From: burns@sasa.gov.uk (Robert Burns) Subject: plant viruses (additional) Date: 2 Aug 1995 09:52:51 -0700 Sender: daemon@net.bio.net Message-ID: NNTP-Posting-Host: net.bio.net Whoops, should have defined PVV in my previous post. It is Potato Virus V Thanks Robert Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk =01=01=01=01 Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Thu Aug 03 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm!cs.utexas.edu!news.sprintlink.net!crash!kiehl From: b3748@cts.com (Bryan Kiehl) Subject: Re: conjugate stability Organization: CTSNET Date: Thu, 3 Aug 1995 22:29:06 GMT Message-ID: <3vriji$1s88_001@kiehl.cts.com> X-Newsreader: News Xpress Version 1.0 Beta #3 References: <9507028073.AA807368724@mail.fws.gov> Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: kiehl.cts.com Lines: 34 > >We routinely make monoclonal AP conjugates by thiolation of the >antibody followed by reaction with activated AP. We find that thge >storage conditions for each conjugate is dependent on the Mab used. > >Some conjugates store well at 4C, some don't. All of them store well >at -20C. We have also been looking at a Pierce product called >superfreeze which allows liquid storage of conjugates at -20C which >should increase longevity. We find that some conjugates store better >with it and some without. BTW in some cases conjugates stored with >superfreeze had decreased activity compared to aliquots of the same >batch stored at 4C without it! > >I think with Mab conjugates it all depends on the parent antibody. > > >Anybody else got any conjugate stories? > I would agree with the above. Polyclonals have been consistent in my experience and can typically be stored in the refrigerator for extended periods if well preserved and start out sterile. Mabs are sometimes a different story. In fact I found that one Mab, a IgG3, usually precipitated out on repeated freeze thaw and was also unstable in the refrigerator. My preservative of choice is sodium azide for alkaline phosphatase conjugates or any reagent if the azide doesn't interfer. You can't use azide with peroxidase conjugates and I recommend using ProClin. Bryan Kiehl GenBio 15222-A Avenue of Science San Diego, CA 92128 (619) 592-9300, ext 309 (619) 592-9400 [FAX] From owner-diagnostics@net.bio.net Fri Aug 04 23:00:00 1995 Path: biosci!agate!news.duke.edu!solaris.cc.vt.edu!news.bluesky.net!news.sprintlink.net!newsfeed.internetmci.com!uunet!in1.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: nadagen@aol.com (Nadagen) Newsgroups: bionet.diagnostics Subject: Re: Problems with DNA based technology Date: 5 Aug 1995 18:04:46 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 4 Sender: root@newsbf02.news.aol.com Message-ID: <400ptu$r1b@newsbf02.news.aol.com> References: <3vido4$hnf@mserv1.dl.ac.uk> Reply-To: nadagen@aol.com (Nadagen) NNTP-Posting-Host: newsbf02.mail.aol.com My laboratory has had a slight problem with the logistics of running PCR because of the ribonuclease destroying the m-RNA before the appropriate info can be gathered....do you know how to stabilize this or have any suggestions From owner-diagnostics@net.bio.net Sat Aug 05 23:00:00 1995 Path: biosci!SIAL.COM!douglasm From: douglasm@SIAL.COM (Michael Douglas) Newsgroups: bionet.diagnostics Subject: Re: Problems with DNA based technology Date: 6 Aug 1995 13:25:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <400ptu$r1b@newsbf02.news.aol.com> NNTP-Posting-Host: net.bio.net It is essential with these sensitive methodologies that everything including glassware, plasticware and reagents be RNAse free. Treat all reagents and solutions with DEPC (diethylpyrocarbonate), wear gloves use good laboratory practice as if sterile and pray. MGD ___________________________________________________________________________ Michael Douglas douglasm@sial.com Sigma Diagnostics -- 545 South Ewing -- St. Louis, MO 63103 USA FAX: 314-531-2586 or 800-816-1603 Tele: 800-521-8956 or 314-771-5765 x4435 On 5 Aug 1995, Nadagen wrote: > My laboratory has had a slight problem with the logistics of running PCR > because of the ribonuclease destroying the m-RNA before the appropriate > info can be gathered....do you know how to stabilize this or have any > suggestions > > From owner-diagnostics@net.bio.net Mon Aug 07 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!nac.no!Norway.EU.net!EU.net!newsfeed.internetmci.com!gatech!howland.reston.ans.net!ix.netcom.com!netnews From: t-bone13@ix.netcom.com (Dan Tisone ) Newsgroups: bionet.diagnostics Subject: Production Equipment for Rapid Test Kits Date: 8 Aug 1995 15:29:34 GMT Organization: Netcom Lines: 17 Message-ID: <407vsu$kfd@ixnews3.ix.netcom.com> NNTP-Posting-Host: ix-ir16-13.ix.netcom.com Our company's product line includes electric cutters, clamshell and reel-to-reel laminators, strip and reel-to-reel X/Y positioning/dispensing tables, and various dispensers ranging from aerosol, to solenoid, to inkjet. These products can be used in the R&D process as well as in a full time manufacturing enviornment. We also provide consulting services on how to develop rapid diagnostics, immunoassays, and dry chemistries. If we can provide any assistance, please contact me through my internet address and I can send more information. Daniel M. Tisone t-bone13@ix.netcom.com BioDot, Inc Irvine, CA USA From owner-diagnostics@net.bio.net Mon Aug 07 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!howland.reston.ans.net!swrinde!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!news.belwue.de!news.dfn.de!uni-muenster.de!news From: Torsten Boerchers Newsgroups: bionet.diagnostics Subject: Re: Storage of conjugates Date: 8 Aug 1995 17:46:38 GMT Organization: ICB Lines: 39 Message-ID: <4087tu$16kk@majestix.uni-muenster.de> References: <199508010812.BAA11589@net.bio.net> <3vmkmb$1s4k_001@kiehl.cts.com> NNTP-Posting-Host: arnheim.uni-muenster.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) b3748@cts.com (Bryan Kiehl) wrote: >In article <199508010812.BAA11589@net.bio.net>, > vigfrid.ness@veths.no (Vigfrid Ness) wrote: >>Hi >>Can anybody tell me the best way to store conjugated antibodies???? >> >>Viffi Ness >>DYNAL AS >> >Conjugates (alkaline phosphatase or peroxidase), assuming they were prepared >properly, can be stored at refrigeration temperatures for many months and are >stable frozen for years. In most cases, the reason for eventual inactivity is >microbial contamination. > >Bryan Kiehl >GenBio >15222-A Avenue of Science >San Diego, CA 92128 >(619) 592-9300, ext 309 >(619) 592-9400 [FAX] IMHO storage conditions cannot be generalized. They may as well depend on the antibody, the stoichiometry of conjugation and the method of coupling. But remember, do not use sodium azide when storing peroxidase conjugates!! A good alternative in this case is Thimerosal. Torsten -------------------------------------------------------------------------/ Torsten Boerchers / _/ _/_/_/ _/_/_/ Institute of Chemical- and Biochemical Sensor/ _/ _/ _/ _/ Research (Molecular Biology Group) / _/ _/ _/_/_/ Mendelstr 7, D-48149 Muenster, Germany / _/ _/ _/ _/ Fax: +49-251-980 2890 Phone: +49-251-980 2876/ _/ _/_/_/ _/_/_/_/ E-Mail: borcher@uni-muenster.de / ------------------------------------------------------------------/ From owner-diagnostics@net.bio.net Mon Aug 07 23:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: Production Equipment for Rapid Test Kits Date: 8 Aug 1995 09:25:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 97 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <407vsu$kfd@ixnews3.ix.netcom.com> NNTP-Posting-Host: net.bio.net > > Our company's product line includes [snip] These products can be used = in the R&D > process as well as in a full time manufacturing enviornment. . Commercial postings of this kind are not allowed on bionet. See the = extract below from the FAQ. If you wish to advertise your products on = the net, I suggest that you either buy some web space or subscribe to = something like biz-biotech.: > WHAT ARE THE RESTRICTIONS ON COMMERCIAL ACTIVITIES IN THE BIOSCI/BIONE= T > NEWSGROUPS? > >BIOSCI is funded in part by the National Science Foundation (NSF) >which supports the Internet in the U.S. with U.S. taxpayer dollars. >NSF is responsible for setting Acceptable Use Policy (AUP) for the the >NSFNet backbone section of the Internet of which BIOSCI makes >extensive use. Because of these reasons, BIOSCI users should adhere to >the following guidelines. > >Commercial activities on BIOSCI are in general prohibited except as >noted below. People at for-profit organizations are free to read all >postings made to the BIOSCI/bionet newsgroups, but must ensure that >their postings to the newsgroups do not violate our guidelines. > >Commercial organizations may post job openings on >EMPLOYMENT/bionet.jobs subject to the format restrictions for that >group. Commercial job posting format restrictions for the >EMPLOYMENT/bionet.jobs newsgroup are described above under the >question "Where should I post my messages?" Users who violate these >format restrictions consciously risk losing their network access. > >BIOSCI readers without any financial connections to a company or a >product may discuss and/or post endorsements of a commercial product. >However, it is standard Internet practice to include in the posting a >disclaimer of any financial interest in the product/company. Note that >postings to newsgroups are subject to libel laws. BIOSCI advises >readers to think twice before taking potshots at products that they do >not like. > >BIOSCI users often post general questions about problems that might be >solved through the use of a commercial product. It is EXTREMELY >IMPORTANT, however, that such general questions *not* be answered by >people affiliated with the product or company that might stand to gain >a sale as a result. For example, user X may ask, "Is there a product >that will allow me to separate protein A from protein B given the >following properties ...?", but, if user Y works at company Z which >sells a product that can accomplish this task, user Y does *NOT* have >permission to respond to the question. Responses can only be posted by >other scientists who might have experience in solving the problem in >question and who do not stand to gain financially by promoting the >product in question, i.e., they are not employees, consultants, or >connected to the company via other financial ties. As noted above, >postings endorsing commercial products should contain a customary >disclaimer stating the absence of financial ties of the poster to the >product/company. > >Commercial companies MAY RESPOND to a public BIOSCI newsgroup if a >BIOSCI user asks a question directly about one of their products, >e.g., mentions it by name. The response should be limited to a factual >answer of the question posed and should avoid any hint of advertising >hype. Comparisons with competitors' products should be avoided >completely. > >Finally, as a general rule, if you are unsure about the >appropriateness of your posting, before you post anything please send >a copy of what you propose to post to the BIOSCI adminstrator at >biosci-help@net.bio.net for review. > >BIOSCI will takes steps to terminate network access to any reader who >willfully violates our commercial use policies. > > > Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 08 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.dgsys.com!kpl From: Mblank@kpl.com (Martin Blankfard) Newsgroups: bionet.diagnostics Subject: Re: conjugate stability Date: 7 Aug 1995 15:23:36 GMT Organization: KPL Lines: 72 Distribution: world Message-ID: <405ccf$q5k_001@dgs.dgsys.com> References: <9507028073.AA807368724@mail.fws.gov> NNTP-Posting-Host: kpl.com X-Newsreader: News Xpress Version 1.0 Beta #4 In article <9507028073.AA807368724@mail.fws.gov>, Mail-9DE~SMTP@MAIL.FWS.GOV wrote: > >User coler is not defined > >Received: from net.bio.net by mail.fws.gov (SMTPLINK V2.10.03) >X-Envelope-From: BIOSCI-REQUEST@net.bio.net >Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id >EAA29200 for diagnost-list; Wed, 2 Aug 1995 04:29:59 -0700 >Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id >EAA29192 for diagnost-arpanet; Wed, 2 Aug 1995 04:29:56 -0700 >To: diagnost@net.bio.net >From: burns@sasa.gov.uk (Robert Burns) >Subject: storage of conjugates >Date: 2 Aug 1995 04:29:45 -0700 >Sender: daemon@net.bio.net >Message-ID: > >NNTP-Posting-Host: net.bio.net > >We routinely make monoclonal AP conjugates by thiolation of the >antibody followed by reaction with activated AP. We find that thge >storage conditions for each conjugate is dependent on the Mab used. > >Some conjugates store well at 4C, some don't. All of them store well >at -20C. We have also been looking at a Pierce product called >superfreeze which allows liquid storage of conjugates at -20C which >should increase longevity. We find that some conjugates store better >with it and some without. BTW in some cases conjugates stored with >superfreeze had decreased activity compared to aliquots of the same >batch stored at 4C without it! > >I think with Mab conjugates it all depends on the parent antibody. > > >Anybody else got any conjugate stories? > > >Robert > > > >Robert Burns > >Monoclonal Antibody Unit >Scottish Agricultural Science Agency >East Craigs >Edinburgh >Scotland > >burns@sasa.gov.uk > Most conjugates can be made stable at 4C with 50% Glycerol in Picopure water. This works for both poly and monoclonal conjugates with a variety of enzymes as long as the protein concentration is at or above 1mg/ml. Another reasonable stabilizer is 1% fish gelatin. Fat oxidation and free radicals can be important when obtaining fish gelatin. Martin Blankfard Senior Scientist Kirkegaard and Perry Laboratories 2 Cessna Ct. Gaithersburg Md , 20879 Phone: (US) 1-800-638-3167 x141 FAX : 301 948 9442 E-mail: Mblank@kpl.com 08/07/95 11:41:57 From owner-diagnostics@net.bio.net Wed Aug 09 23:00:00 1995 Path: biosci!BEPC2.IHEP.AC.CN!wangyq From: wangyq@BEPC2.IHEP.AC.CN Newsgroups: bionet.diagnostics Subject: HCG Date: 10 Aug 1995 06:56:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <00994AF4.34BB5BE0.40@bepc2.ihep.ac.cn> NNTP-Posting-Host: net.bio.net Dear netter: I want to buy large quantity of Bata-HCG ascites for immunodiagnosis. It must have no cross-reaction with LH, FSH and TSH. Any information would be very appreciated. Thank you. Wang Yuquan Beijing Cons Bio-Tech From owner-diagnostics@net.bio.net Thu Aug 10 23:00:00 1995 Path: biosci!KISTMAIL.KIST.RE.KR!hyunpark From: hyunpark@KISTMAIL.KIST.RE.KR (Park Hyun) Newsgroups: bionet.diagnostics Subject: (none) Date: 11 Aug 1995 01:18:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <9508110815.AA19766@kistmail.kist.re.kr> NNTP-Posting-Host: net.bio.net unsubscribe list From owner-diagnostics@net.bio.net Thu Aug 10 23:00:00 1995 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!nntp-hub2.barrnet.net!news.Stanford.EDU!not-for-mail From: adhar@leland.Stanford.EDU (Adam Noel Harris) Newsgroups: bionet.diagnostics,bionet.drosophila Subject: Re: Hello, talk to me Followup-To: bionet.diagnostics,bionet.drosophila Date: 11 Aug 1995 10:45:15 -0700 Organization: Stanford University Lines: 7 Message-ID: <40g4vb$s4n@elaine46.Stanford.EDU> References: <094424Z11081995@anon.penet.fi> NNTP-Posting-Host: elaine46.stanford.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.diagnostics:247 bionet.drosophila:1290 Remember to send your complaints to admin@anon.penet.fi Just a friendly reminder. -Adam PGP Fingerprint = C0 65 A2 BD 8A 67 B3 19 F9 8B C1 4C 8E F2 EA 0E From owner-diagnostics@net.bio.net Thu Aug 10 23:00:00 1995 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.sprintlink.net!in2.uu.net!panix!not-for-mail From: reinitz@panix.com (John Reinitz) Newsgroups: bionet.diagnostics,bionet.drosophila Subject: Re: Hello, talk to me Date: 11 Aug 1995 17:20:51 -0400 Organization: Panix Lines: 25 Message-ID: <40ghjj$c4a@panix2.panix.com> References: <094424Z11081995@anon.penet.fi> NNTP-Posting-Host: panix2.panix.com Xref: biosci bionet.diagnostics:248 bionet.drosophila:1294 I sent the following to admin@anon.penet.fi about the chain letter spam, along with a copy. an350917@anon.penet.fi will now lose access to the anon server. Julf, Sorry to bother you, but an350917@anon.penet.fi is spamming USENET with the following post to bionet.drosophila and bionet.diagnostics. This is a clear abuse. Chain letters are illegal in the United States, and probably in most other places as well. >---------------------------------------------------------------------------- >To find out more about the anon service, send mail to help@anon.penet.fi. >If you reply to this message, your message WILL be *automatically* anonymized >and you are allocated an anon id. Read the help file to prevent this. >Please report any problems, inappropriate use etc. to admin@anon.penet.fi. -- John Reinitz v:212-241-1952 f:212-860-9279 USnail: Brookdale Center for Molecular Bio., Box 1126 Mt. Sinai Med. School, One Gustave L. Levy Place, NYC, NY 10029 USA. I don't speak for/from Mt. Sinai. "Absolute secrecy corrupts absolutely"--current CIA inspector-general. From owner-diagnostics@net.bio.net Fri Aug 11 23:00:00 1995 Path: biosci!KISTMAIL.KIST.RE.KR!hyunpark From: hyunpark@KISTMAIL.KIST.RE.KR (Park Hyun) Newsgroups: bionet.diagnostics Subject: diagnost@net.bio.net Date: 11 Aug 1995 17:53:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <9508120050.AA24180@kistmail.kist.re.kr> NNTP-Posting-Host: net.bio.net unsubscribe list From owner-diagnostics@net.bio.net Mon Aug 14 23:00:00 1995 Path: biosci!SIAL.COM!patersot From: patersot@SIAL.COM (Tom Paterson) Newsgroups: bionet.diagnostics Subject: subscribe Date: 15 Aug 1995 14:09:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe diagnost From owner-diagnostics@net.bio.net Tue Aug 15 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!geraldo.cc.utexas.edu!usenet From: "Greg W. Peterson" Newsgroups: bionet.diagnostics Subject: Re: diagnost@net.bio.net Date: 16 Aug 1995 17:12:01 GMT Organization: The University of Texas at Austin Lines: 3 Message-ID: <40t8t1$c0@geraldo.cc.utexas.edu> References: <9508120050.AA24180@kistmail.kist.re.kr> NNTP-Posting-Host: smf-b15.facsmf.utexas.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: hyunpark@KISTMAIL.KIST.RE.KR X-URL: news:9508120050.AA24180@kistmail.kist.re.kr subscribe: bionet.diagnostics From owner-diagnostics@net.bio.net Wed Aug 16 23:00:00 1995 Path: biosci!INDIRECT.COM!coburn From: coburn@INDIRECT.COM (Anne Coburn) Newsgroups: bionet.diagnostics Subject: gopher://ftp.bio.indiana.edu:70/00/Network-News/bionet/diagnostics/Apr95.news Date: 17 Aug 1995 05:55:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 117 Sender: daemon@net.bio.net Distribution: world Message-ID: <199508171253.FAA28990@bud.indirect.com> NNTP-Posting-Host: net.bio.net Hello, I hope that this posting isn't inappropriate. I'm sure I read that job postings were OK. I'm a headhunter and I currently have two retained searchs for which scientist's in this news group might be interested. Both of these are IMMEDIATE openings in an independent business unit for a company, located in the SF Bay Area, that is known worldwide for quality and support. The BIOSCIENCE PRODUCTS GROUP, is dedicated to the development of molecular recognition, with the initial focus being a product for DNA recognition using microscopic arrays of fluorescently tagged probes. These positions offer a unique chance to work in a largely entrepreneurial environment, while still enjoying the benefits of working for a large and stable company. MANAGER - DIAGNOSTICS PRODUCT DEVELOPOMENT OBJECTIVES - Provide the experience, maturity and focus necessary to successfully guide this newly formed applied research/applied development group composed of both Ph.D.'s and engineers. - Serve as the primary interface between various functions within the BioScience Group, as well as between this business unit and central research, corporate management, and areas relevant to, but not in, this area. - Facilitate the timely development of a significant first product by using prior experience to take "interesting science" from R&D, through product development to commercialization. REQUIREMENTS - Ph.D. in Molecular Biology or closely related area. Established contacts with academic leaders and prinicple innovators would be attractive. - 7+ years of industrial experinece (MUST HAVE!), with significant emphasis on NUCLEIC ACID and DIAGNOSTIC PRODUCT DEVELOPMENT, some from new technologies. Some exposure to technology evaluation and acquisition would be an asset. - Management experience of at least one group of applied R&D scientist's. An applicant who has started, staffed, and managed a new group will have a special attraction. - Technical expertise in nucleic acids supported by an in-depth under- standing of the dignostics marketplace; some background involving an instrument as part of a product previouslyh developed; knowledge of manufacturing of chemistry based consumables, and a familiarity with the processes for both FDA approval and clinical trials. - Flexibility, strong organizational and administrative skills, and an enthusiasm for team work AND hard work. PRODUCT MARKETING MANAGER - In Vitro DIAGNOSTICS REQUIREMENTS - BS in a biological or biochemical science. - 5+ years of relevant experience in the biomedical and diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of market drivers and market segmentations in the IVD market. - Demonstrated knowledge of market drivers and market segmentations of - the IVD market. - Direct experience in selling, supporting or marketing successful product systems to the IVD market. - Demonstrated and applied customer-focused market research tools for the development of new product systems in the market. - Ability to perform competitive analysis on both product features and benefits and the related positioning of product systems in the market. - Effective communication skills - Knowledge of medical device regulations. - MUST be a team player and able to work independently with minimum supervision. PRODUCT MARKETING MANAGER - In Vitro OBJECTIVES; - Originate and execute market research and marketing programs. - Identify and characterize market opportunities. - Recommend a comprehensive strategy for market entry and development in the IVD area. - Identify and develop specific product opportunities and define strategic direction for biomolecular measurements in diagnostics. - Work closely with R&D to formulate strategies. REQUIREMENTS - BS in a biological or biochemical science. - 5+ years' relevant experience in the biomedical or diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of diagnostic customer needs, applications and market trends. - Demonstrated knowledge of market drivers and market segmentations in the IVD market. - Direct experience in selling, supporting, or marketing successful product systems to the IVD markets. For immediate, and CONFIDENTIAL consideration, contact: ANNE COBURN Coburn Scientific Search 4449 East Whitman Tucson, AZ 85711-3515 Ph: 881.0084 Fax: 520.321.1475 e-mail: coburn@indirect.com From owner-diagnostics@net.bio.net Wed Aug 16 23:00:00 1995 Path: biosci!INDIRECT.COM!coburn From: coburn@INDIRECT.COM (Anne Coburn) Newsgroups: bionet.diagnostics Subject: gopher://ftp.bio.indiana.edu:70/00/Network-News/bionet/diagnostics/Apr95.news Date: 17 Aug 1995 05:55:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 117 Sender: daemon@net.bio.net Distribution: world Message-ID: <199508171253.FAA29001@bud.indirect.com> NNTP-Posting-Host: net.bio.net Hello, I hope that this posting isn't inappropriate. I'm sure I read that job postings were OK. I'm a headhunter and I currently have two retained searchs for which scientist's in this news group might be interested. Both of these are IMMEDIATE openings in an independent business unit for a company, located in the SF Bay Area, that is known worldwide for quality and support. The BIOSCIENCE PRODUCTS GROUP, is dedicated to the development of molecular recognition, with the initial focus being a product for DNA recognition using microscopic arrays of fluorescently tagged probes. These positions offer a unique chance to work in a largely entrepreneurial environment, while still enjoying the benefits of working for a large and stable company. MANAGER - DIAGNOSTICS PRODUCT DEVELOPOMENT OBJECTIVES - Provide the experience, maturity and focus necessary to successfully guide this newly formed applied research/applied development group composed of both Ph.D.'s and engineers. - Serve as the primary interface between various functions within the BioScience Group, as well as between this business unit and central research, corporate management, and areas relevant to, but not in, this area. - Facilitate the timely development of a significant first product by using prior experience to take "interesting science" from R&D, through product development to commercialization. REQUIREMENTS - Ph.D. in Molecular Biology or closely related area. Established contacts with academic leaders and prinicple innovators would be attractive. - 7+ years of industrial experinece (MUST HAVE!), with significant emphasis on NUCLEIC ACID and DIAGNOSTIC PRODUCT DEVELOPMENT, some from new technologies. Some exposure to technology evaluation and acquisition would be an asset. - Management experience of at least one group of applied R&D scientist's. An applicant who has started, staffed, and managed a new group will have a special attraction. - Technical expertise in nucleic acids supported by an in-depth under- standing of the dignostics marketplace; some background involving an instrument as part of a product previouslyh developed; knowledge of manufacturing of chemistry based consumables, and a familiarity with the processes for both FDA approval and clinical trials. - Flexibility, strong organizational and administrative skills, and an enthusiasm for team work AND hard work. PRODUCT MARKETING MANAGER - In Vitro DIAGNOSTICS REQUIREMENTS - BS in a biological or biochemical science. - 5+ years of relevant experience in the biomedical and diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of market drivers and market segmentations in the IVD market. - Demonstrated knowledge of market drivers and market segmentations of - the IVD market. - Direct experience in selling, supporting or marketing successful product systems to the IVD market. - Demonstrated and applied customer-focused market research tools for the development of new product systems in the market. - Ability to perform competitive analysis on both product features and benefits and the related positioning of product systems in the market. - Effective communication skills - Knowledge of medical device regulations. - MUST be a team player and able to work independently with minimum supervision. PRODUCT MARKETING MANAGER - In Vitro OBJECTIVES; - Originate and execute market research and marketing programs. - Identify and characterize market opportunities. - Recommend a comprehensive strategy for market entry and development in the IVD area. - Identify and develop specific product opportunities and define strategic direction for biomolecular measurements in diagnostics. - Work closely with R&D to formulate strategies. REQUIREMENTS - BS in a biological or biochemical science. - 5+ years' relevant experience in the biomedical or diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of diagnostic customer needs, applications and market trends. - Demonstrated knowledge of market drivers and market segmentations in the IVD market. - Direct experience in selling, supporting, or marketing successful product systems to the IVD markets. For immediate, and CONFIDENTIAL consideration, contact: ANNE COBURN Coburn Scientific Search 4449 East Whitman Tucson, AZ 85711-3515 Ph: 881.0084 Fax: 520.321.1475 e-mail: coburn@indirect.com From owner-diagnostics@net.bio.net Wed Aug 16 23:00:00 1995 Path: biosci!INDIRECT.COM!coburn From: coburn@INDIRECT.COM (Anne Coburn) Newsgroups: bionet.diagnostics Subject: gopher://ftp.bio.indiana.edu:70/00/Network-News/bionet/diagnostics/Apr95.news Date: 17 Aug 1995 05:55:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 117 Sender: daemon@net.bio.net Distribution: world Message-ID: <199508171253.FAA28979@bud.indirect.com> NNTP-Posting-Host: net.bio.net Hello, I hope that this posting isn't inappropriate. I'm sure I read that job postings were OK. I'm a headhunter and I currently have two retained searchs for which scientist's in this news group might be interested. Both of these are IMMEDIATE openings in an independent business unit for a company, located in the SF Bay Area, that is known worldwide for quality and support. The BIOSCIENCE PRODUCTS GROUP, is dedicated to the development of molecular recognition, with the initial focus being a product for DNA recognition using microscopic arrays of fluorescently tagged probes. These positions offer a unique chance to work in a largely entrepreneurial environment, while still enjoying the benefits of working for a large and stable company. MANAGER - DIAGNOSTICS PRODUCT DEVELOPOMENT OBJECTIVES - Provide the experience, maturity and focus necessary to successfully guide this newly formed applied research/applied development group composed of both Ph.D.'s and engineers. - Serve as the primary interface between various functions within the BioScience Group, as well as between this business unit and central research, corporate management, and areas relevant to, but not in, this area. - Facilitate the timely development of a significant first product by using prior experience to take "interesting science" from R&D, through product development to commercialization. REQUIREMENTS - Ph.D. in Molecular Biology or closely related area. Established contacts with academic leaders and prinicple innovators would be attractive. - 7+ years of industrial experinece (MUST HAVE!), with significant emphasis on NUCLEIC ACID and DIAGNOSTIC PRODUCT DEVELOPMENT, some from new technologies. Some exposure to technology evaluation and acquisition would be an asset. - Management experience of at least one group of applied R&D scientist's. An applicant who has started, staffed, and managed a new group will have a special attraction. - Technical expertise in nucleic acids supported by an in-depth under- standing of the dignostics marketplace; some background involving an instrument as part of a product previouslyh developed; knowledge of manufacturing of chemistry based consumables, and a familiarity with the processes for both FDA approval and clinical trials. - Flexibility, strong organizational and administrative skills, and an enthusiasm for team work AND hard work. PRODUCT MARKETING MANAGER - In Vitro DIAGNOSTICS REQUIREMENTS - BS in a biological or biochemical science. - 5+ years of relevant experience in the biomedical and diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of market drivers and market segmentations in the IVD market. - Demonstrated knowledge of market drivers and market segmentations of - the IVD market. - Direct experience in selling, supporting or marketing successful product systems to the IVD market. - Demonstrated and applied customer-focused market research tools for the development of new product systems in the market. - Ability to perform competitive analysis on both product features and benefits and the related positioning of product systems in the market. - Effective communication skills - Knowledge of medical device regulations. - MUST be a team player and able to work independently with minimum supervision. PRODUCT MARKETING MANAGER - In Vitro OBJECTIVES; - Originate and execute market research and marketing programs. - Identify and characterize market opportunities. - Recommend a comprehensive strategy for market entry and development in the IVD area. - Identify and develop specific product opportunities and define strategic direction for biomolecular measurements in diagnostics. - Work closely with R&D to formulate strategies. REQUIREMENTS - BS in a biological or biochemical science. - 5+ years' relevant experience in the biomedical or diagnostic industry. - Familiarity with current clinical diagnostic applications and trends. - Understanding of diagnostic customer needs, applications and market trends. - Demonstrated knowledge of market drivers and market segmentations in the IVD market. - Direct experience in selling, supporting, or marketing successful product systems to the IVD markets. For immediate, and CONFIDENTIAL consideration, contact: ANNE COBURN Coburn Scientific Search 4449 East Whitman Tucson, AZ 85711-3515 Ph: 881.0084 Fax: 520.321.1475 e-mail: coburn@indirect.com From owner-diagnostics@net.bio.net Wed Aug 16 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!in1.uu.net!tank.news.pipex.net!pipex!warwick!news.ncl.ac.uk!usenet From: Simon Eaton Newsgroups: bionet.diagnostics Subject: Inborn Errors Mailing List Date: 17 Aug 1995 17:03:26 GMT Organization: Newcastle University, UK Lines: 25 Message-ID: <40vsou$pnn@whitbeck.ncl.ac.uk> NNTP-Posting-Host: russet.ncl.ac.uk Mime-Version: 1.0 X-Newsreader: WinVN 0.99.4 There has been a mailing list set up for the discussion of inborn errors of metabolism. . We intend to set up a newsgroup eventually but in order to gauge support, have decided to set up a mailbase newsgroup first. The intention is to provide a discussion of research into Inherited Metabolic Diseases, which can be important causes of disease in both children and adults. The discussion will include: a) Biochemistry and Diagnosis b) Genetics c) Clinical, nutritional and treatment aspects d) General discussion. Joining inborn-errors To join inborn-errors send the command: Join inborn-errors firstname(s) lastname (substituting appropriately) as the only text in the body of a message addressed to: mailbase@mailbase.ac.uk For any queries relating to the list, contact the owner at: inborn-errors-request@mailbase.ac.uk For general information relating to Mailbase, see http://www.mailbase.ac.uk/ You can get to the inborn-errors list page from this URL, or directly using the URL gopher://nisp.ncl.ac.uk:70/11/lists-f-j/inborn-errors From owner-diagnostics@net.bio.net Thu Aug 17 23:00:00 1995 Path: biosci!bcm!news.msfc.nasa.gov!europa.chnt.gtegsc.com!news.mathworks.com!newshost.marcam.com!usc!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!pcdnr05.uio.no!user From: olam@radium.uio.no (Ola Myklebost) Newsgroups: bionet.diagnostics,bit.med.mxdiag-l,sci.med.pathology Subject: Experiences with lab standards for genetic testing? Date: 18 Aug 1995 06:57:55 GMT Organization: Inst for Cancer Research, Oslo Lines: 34 Message-ID: NNTP-Posting-Host: pcdnr05.uio.no Xref: biosci bionet.diagnostics:256 sci.med.pathology:1185 In Norway we have a new law on medical applications of biotechnology, where there is opportunity to regulate artificial fertilization, preimplantation diagnostics, pre- and postnatal genetic testing and gene therapy. We are currently discussing the criteria for allowing a lab to do genetic tests, and for allowing specific tests. One may require the lab to be accredited, by which their way of performing specific procedures is quality controlled by an external body. By this procedure they have to do tests in a specific way over long periods. An alternative, perhaps better suited for research labs and quickly developing procedures, one may require the lab to get a GLP (Good lab. practise) certificate. In both cases an external body will irregularly visit the lab, check the records and the way work is done. What is the experience in other countries, have quality controls been enforced in this way anywhere? I think some Dutch and British labs have been certified. It would be interesting to hear from these (or be pointed towards them). Ola -- Ola Myklebost Email ola.myklebost@labmed.uio.no Dept of Tumor Biology Inst for Cancer Research Tel +47-2293-4299 The Norwegian Radium Hospital Fax +47-2252-2421 N-0310 OSLO, Norway WWW presentation: http://www.med.uio.no/dnr/Inst/TumBiol/Ola/Ola.html From owner-diagnostics@net.bio.net Sat Aug 19 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!crash!kiehl From: b3748@cts.com (Bryan Kiehl) Subject: Human Mycoplasma Serology Organization: CTSNET Date: Sat, 19 Aug 1995 19:11:14 GMT Message-ID: <415d0i$1s4k_001@kiehl.cts.com> X-Newsreader: News Xpress Version 1.0 Beta #3 Sender: news@crash.cts.com (news subsystem) Nntp-Posting-Host: kiehl.cts.com Lines: 20 I am completing field studies of a mycoplasma pnuemonia IgM serology test, but would like to use the assay for prospective studies during the next few months while the FDA reviews our controlled study data. The test is in a mirotiter well format, using purified complement fixing antigen and an anti-human IgG absorbent. We can pay for limited technician time and support either publication and/or limited transportation costs. The comparison needs to be made using at least 10 true positive patients and 20 patients that present with pneumonia, but are later defined not to have M. pneumonia infection. Defininition needs to be by means other than serology. If interested, please let me know. Bryan Kiehl GenBio 15222-A Avenue of Science San Diego, CA 92128 (619) 592-9300, ext 309 (619) 592-9400 [FAX] From owner-diagnostics@net.bio.net Sun Aug 20 23:00:00 1995 Path: biosci!bcm!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!dundee.ac.uk!usenet From: keith appleyard Newsgroups: bionet.diagnostics,bionet.drosophila Subject: Re: Hello, talk to me Date: 21 Aug 1995 11:55:47 GMT Organization: Dundee University Lines: 13 Message-ID: <419s83$bnq@dux.dundee.ac.uk> References: <094424Z11081995@anon.penet.fi> <40ghjj$c4a@panix2.panix.com> NNTP-Posting-Host: pub17.medschool.dundee.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Xref: biosci bionet.diagnostics:259 bionet.drosophila:1322 Earlier this year a research group in California was conducting research into "memes" on the internet. This involves tracing chain mail and monitoring how it changes as time progresses. If you come across any chainmail of this sort send them a copy at meme@scottlabsgi.chem.sfu.ca atthe same time that you complain and ask for it's removal. -- Keith Appleyard BSc, Senior Scientific Officer, Virology Ninewells Hospital and Medical School, Dundee DD1 9SY, U.K, e-mail kappleyard@ninewells.dundee.ac.uk Tel: U.K 01382 662559 Fax: U.K 01382 641907 From owner-diagnostics@net.bio.net Mon Aug 21 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!nntp-trd.UNINETT.no!sunic!sunic.sunet.se!news.sprintlink.net!in1.uu.net!newsflash.concordia.ca!nstn.ns.ca!news.cs.indiana.edu!umn.edu!news From: "lili@ee.umn.edu... Li Li" Subject: Re: Problems with DNA based technology To: NAB@LGC.CO.UK Message-ID: <83545.lili@ee.umn.edu> X-Minuet-Version: Minuet1.0_Beta_16 Sender: news@news.cis.umn.edu (Usenet News Administration) Nntp-Posting-Host: dialup-3-224.gw.umn.edu X-Popmail-Charset: English Organization: University of Minnesota, Twin Cities Date: Tue, 22 Aug 1995 02:24:07 GMT Lines: 26 On 31 Jul 1995 12:11:00 +0100, NAB@LGC.CO.UK wrote: >I am conducting a survey to identify the suitability and >limitations of key DNA based technologies. These include nucleic >acid extraction methods: yield, purity of product, carryover of >chemicals which could inhibit subsequent PCR, one tube protocols >etc. Hybridisation protocols, non-radioactive labelling methods: >relative sensitivity and probe stability etc. I am also >interested in problems associated with all forms of PCR >especially RT-PCR and forensic techniques such as RFLP and STR >profiling, CAP, AFLP etc. Any problems you have experienced and >any suggestions would be more than welcome. I,ve got until the 30 >September 1995 so HELP! > > I read your message. It is interesting. I have an engineering background and I am thinking doing some research on biomedical engineering. The initial step is scaling down the DNA sequencing by micromachined electronical technology. Since this is just start. I hope you can exchange some ideas with me. Li Li Dept. of electrical engineering University of Minnesota From owner-diagnostics@net.bio.net Mon Aug 21 23:00:00 1995 Path: biosci!MENZIES.SU.EDU.AU!antje From: antje@MENZIES.SU.EDU.AU (Antje Haase) Newsgroups: bionet.diagnostics Subject: PCR of blood Date: 22 Aug 1995 00:15:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <9508220720.AA06715@menzies.su.edu.au> NNTP-Posting-Host: net.bio.net Hi everybody, we are trying to detect small numbers of bacteria in blood samples. After having tried a number of methods described in the literature we are not quite happy with the sensitivity. The number of bacteria is far too small to just spin them put and DNA extraction doesn't feel like the way to go either for the same reason. We have tried various membraneswithout success. Does anyone have experience with this problem? Any help or suggestions are greatly appreciated thanks in advance Antje __________________________________________ Antje Haase Menzies School of Health Research Australia email: antje@menzies.su.edu.au __________________________________________ From owner-diagnostics@net.bio.net Mon Aug 21 23:00:00 1995 Path: biosci!NFRSSJ.AGR.CA!HAMPSONM From: HAMPSONM@NFRSSJ.AGR.CA Newsgroups: bionet.diagnostics Subject: viability staining Date: 22 Aug 1995 05:07:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HUD7BMT9F6000EPR@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Hello Netters, I work with the chytridiomycete Synchytrium endobioticum. The overwintering body is a resting spore. This is an obligate (to potato) fungus and reproduces in potato tissue cells. It does not produce a mycelium. It is difficult to tell from light microscopy if the spore is viable or not. Population viability can be estimated somewhat in bioassay with potatoes, but I need a method to identify single spore viability. I have tried many flurochromes - settling on acriflavin - but it is not a surefire system. There is not much that I can find in the literature to help me. Are there some new products out there that I can try? Any suggestion will be much appreciated. Thanks. Mike Hampson (hampsonm@NFRSSJ.agr.ca). From owner-diagnostics@net.bio.net Tue Aug 22 23:00:00 1995 Path: biosci!EM.AGR.CA!BrescianiS From: BrescianiS@EM.AGR.CA (Sandy Bresciani) Newsgroups: bionet.diagnostics Subject: diagnostic kits for mycotoxin analysis Date: 23 Aug 1995 10:22:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To list members: I am looking for the names of companies that produce/market/sell diagnostic kits for the testing of mycotoxins in grains, feed, or food products. I am trying to compile a complete "global" list of such companies. I was wondering if anyone could provide the names of companies involved in this industry as well as any relevant information regarding the company such as country of origin, e-mail address, phone number, etc. Any information would be useful even if it is just the name of a company. Thanks, Sandy Bresciani brescianis@em.agr.ca Agriculture and Agri-Food Canada tel: 306-975-6421 fax: 306-975-6419 From owner-diagnostics@net.bio.net Tue Aug 22 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: Sandy Bresciani Newsgroups: bionet.diagnostics Subject: diagnostic kits for analysing mycotoxins Date: 23 Aug 1995 18:55:09 +0100 Lines: 19 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <41fq1t$oii@mserv1.dl.ac.uk> Original-To: diagnost@dl.ac.uk To list members: I am looking for the names of companies that produce/market/sell diagnostic kits for the testing of mycotoxins in grains, feed, or food products. I am trying to compile a complete "global" list of such companies. I was wondering if anyone could provide the names of companies involved in this industry as well as any relevant information regarding the company such as country of origin, e-mail address, phone number, etc. Any information would be useful even if it is just the name of a company. Thanks, Sandy Bresciani brescianis@em.agr.ca Agriculture and Agri-Food Canada tel: 306-975-6421 fax: 306-975-6419 From owner-diagnostics@net.bio.net Thu Aug 24 23:00:00 1995 Path: biosci!TECHNET.SG!pohyam From: pohyam@TECHNET.SG (Lim Pohyam) Newsgroups: bionet.diagnostics Subject: sample addition indicators Date: 25 Aug 1995 08:22:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199508251520.XAA25193@einstein.technet.sg> NNTP-Posting-Host: net.bio.net I have tried to make IgM ELIsa for syphilis. One problem I encountered was I forget to add in the samples after several hundred tests. Anyone tried to add in colour dyes to eliminate this error? Or are there dyes that are sensitive to pH changes upon addition of sera? Any help would be appreciated. From owner-diagnostics@net.bio.net Thu Aug 24 23:00:00 1995 Path: biosci!agresearch.cri.nz!HartA From: HartA@agresearch.cri.nz Newsgroups: bionet.diagnostics Subject: Drying retardants Date: 24 Aug 1995 21:49:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HUHWMY0YWY004WCP@INVERMAY.CRI.NZ> NNTP-Posting-Host: net.bio.net Suppose I have a thin aqueous film containing enzyme(s). Can anyone suggest a suitable additive(s) which will prevent the film from drying out, without affecting the activity of the enzyme? Thanks, Alan Hart, AgResearch Grasslands. From owner-diagnostics@net.bio.net Thu Aug 24 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!gatech!newsfeed.pitt.edu!dsinc!netnews.upenn.edu!cronkite.ocis.temple.edu!astro.ocis.temple.edu!gold From: gold@astro.ocis.temple.edu (Bert Gold) Newsgroups: bionet.diagnostics Subject: Experiences with lab standards for genetic testing? Date: 25 Aug 1995 21:31:35 GMT Organization: Temple University, Academic Computer Services Lines: 27 Message-ID: <41lffn$ect@cronkite.ocis.temple.edu> NNTP-Posting-Host: astro.ocis.temple.edu X-Newsreader: TIN [version 1.2 PL2] There has been a good deal of experience in the U.S. with this since the implementation of CLIA (The Clinical Laboratory Improvement Act of 1988) in around 1991-92 and with the beginning of Board Certification in Human Molecular Genetics in 1990 and the subsequent exam in 1993. I, myself, served on an inspection team for the College of American Pathologists (in Chicago, which effectively operates as an oversight body through CLIA), in a well-known lab. in Boston. They passed with flying colors, though the insepection was rigorous. One of the reasons I am leaving my current job is that neither my department chairman nor Dean of the Medical School will provide sufficient resources to allow me to pass such a rigorous inspection. Therefore, I'm off to UCSF to become board certified... If you have more questions about the rigors of being a well certified lab, please feel free to call or EMAIL. Yours, Bert Gold, Ph.D. Director, Molecular Pathology Laboratory, Temple U. and (hopefully) Board Eligible Human Molecular Geneticist From owner-diagnostics@net.bio.net Fri Aug 25 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!usenet.eel.ufl.edu!freenet3.freenet.ufl.edu!afn26055 From: "Edward R. Mason" Newsgroups: bionet.diagnostics Subject: Re: diagnostic kits for analysing mycotoxins Date: Sat, 26 Aug 1995 10:17:41 -0400 Lines: 35 Message-ID: References: <41fq1t$oii@mserv1.dl.ac.uk> NNTP-Posting-Host: freenet3.afn.org Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <41fq1t$oii@mserv1.dl.ac.uk> Sandy, One such company would be: ELISA Technologies phone 904-462-4546 fax 904-462-3453 One Progress Blvd., Suite #28 Alachua, FL 32615 Bruce Ritter or Eugene Fueyo can probably help. Best regards, Edward Mason On 23 Aug 1995, Sandy Bresciani wrote: > To list members: > > I am looking for the names of companies that produce/market/sell > diagnostic kits for the testing of mycotoxins in grains, feed, or food > products. I am trying to compile a complete "global" list of such > companies. I was wondering if anyone could provide the names of > companies involved in this industry as well as any relevant information > regarding the company such as country of origin, e-mail address, phone > number, etc. Any information would be useful even if it is just the name of > a company. > > Thanks, > > Sandy Bresciani > brescianis@em.agr.ca > Agriculture and Agri-Food Canada > tel: 306-975-6421 > fax: 306-975-6419 > > > From owner-diagnostics@net.bio.net Sun Aug 27 23:00:00 1995 Path: biosci!idbiomed.com!FouziB From: FouziB@idbiomed.com (Fouzi Bekkaoui) Newsgroups: bionet.diagnostics Subject: Subscribe Date: 28 Aug 1995 14:00:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <30422DD7@mailbox.electric.net> NNTP-Posting-Host: net.bio.net From owner-diagnostics@net.bio.net Mon Aug 28 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test please ignore Date: 29 Aug 1995 06:19:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Mon Aug 28 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: enzyme kinetics in ELISA Date: 29 Aug 1995 02:11:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Message from Isla Browning via Robert Burns( my E mail is out of order) My son is writing up a school project in which he carried out experiments to calculate the Km of alkaline phosphatase acting on p-nitrophenylphosphate in both ELISA, and when the enzyme is free in solution. The experiments were carried out at 25C and at a pH of 9.8. The result was Km= 0.246 for the immobilised enzyme and 0.505 for the free solution. I would be grateful for any comments on these results. It means that there was greater affinity of the enzyme for the substrate when it was immobilised, which is not what we expected. Isla Browning Diagnostics and Molecular Biology Section SASA Edinburgh Tel 0131 244 8916 Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Mon Aug 28 23:00:00 1995 Path: biosci!aol.com!PandaGroup From: PandaGroup@aol.com Newsgroups: bionet.diagnostics Subject: TECHSCAN (sm) Services Date: 28 Aug 1995 20:27:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Distribution: world Message-ID: <950828232455_85823571@mail02.mail.aol.com> NNTP-Posting-Host: net.bio.net TECHSCAN (sm) SERVICES OFFERED WORLDWIDE The Panda Group, Inc. announces the availability of its TECHSCAN (sm) services for companies in the biomedical and biotechnology industries, including diagnostics, pharmaceuticals, biopharmaceuticals, biologicals, etc. TECHSCAN (sm) is a technology assessment service by which participants in international biomedical and biotechnology industries can keep abreast of the latest technology developments arising in the United States and Canada. Our consultants will survey the scientific, technical, and industrial literature concerning your interests. They will then assess the scientific and commercial potential of each finding, and report to you on the key progress in your areas of interest, along with summaries and comments on each item. TECHSCAN (sm) is a convenient and efficient means of monitoring technological advances made in research centers, hospitals, universities, and by competitors. Unlike literature searches, TECHSCAN (sm) provides you with only prescreened information based on your search criteria and the technical expertise of our consultants, and cogent commentary on each entry. It can provide the basis for the acquisition and licensing of new technology developed in North America. TECHSCAN (sm) is also a proprietary service. That is, The Panda Group, Inc. will not perform searches and assessments of the same technology for companies in direct competition in the same markets. You can, therefore, be assured that the information you pay for will provide you with a market edge over your competition. In addition, TECHSCAN (sm) subscribers have access to the complete consulting capabilities of The Panda Group, Inc. for further investigation of selected technologies and initiation of licensing/acquisition contacts in the United States and Canada. TECHSCAN (sm) can provide companies outside of North America with an important source of technological intelligence and opportunity. For further information, e-mail us at PandaGroup@aol.com or phone/fax our offices at 1-619-436-4341. ===================== Founded in 1983, The Panda group, Inc. is a Southern California- based, internationally recognized consulting firm specializing in the biomedical and biotechnology industries. It's client list includes many well-know multinational corporations as well as smaller biomedical and biotechnology companies. From owner-diagnostics@net.bio.net Mon Aug 28 23:00:00 1995 Path: biosci!IX.NETCOM.COM!borschel From: borschel@IX.NETCOM.COM (Richard Borschel) Newsgroups: bionet.diagnostics Subject: Searching for Lamin A,B,C Date: 29 Aug 1995 10:10:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199508291631.JAA27291@ix4.ix.netcom.com> NNTP-Posting-Host: net.bio.net I am looking for help in finding purified Lamin A, B, and C and any Mab's specific for them. I appreciate any help I can get on this as I would like to avoid purifying them myself. -- Major Richard Borschel E-mail: borschel@ix.netcom.com Chief, Research Operstions Service Department of Clinical Investigation Walter Reed Army Medical Center Phone: (202) 782-0120 Washington, DC 20307 FAX: (202) 782-3286 From owner-diagnostics@net.bio.net Tue Aug 29 23:00:00 1995 Path: biosci!MAIL.FWS.GOV!Mail-9DE~SMTP From: Mail-9DE~SMTP@MAIL.FWS.GOV Newsgroups: bionet.diagnostics Subject: enzyme kinetics Date: 30 Aug 1995 02:08:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <9507298097.AA809717152@mail.fws.gov> NNTP-Posting-Host: net.bio.net Message from Isla Browning via Robert Burns( my E mail is out of order) My son is writing up a school project in which he carried out experiments to calculate the Km of alkaline phosphatase acting on p-nitrophenylphosphate in both ELISA, and when the enzyme is free in solution. The experiments were carried out at 25C and at a pH of 9.8. The result was Km= 0.246 for the immobilised enzyme and 0.505 for the free solution. I would be grateful for any comments on these results. It means that there was greater affinity of the enzyme for the substrate when it was immobilised, which is not what we expected. Isla Browning Diagnostics and Molecular Biology Section SASA Edinburgh Tel 0131 244 8916 Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Aug 29 23:00:00 1995 Path: biosci!sasa.gov.uk!browning From: browning@sasa.gov.uk (Isla Browning) Newsgroups: bionet.diagnostics Subject: Enzyme kinetics in ELISA Date: 30 Aug 1995 06:55:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net My son is writing up a school project in which he carried out experiments to calculate the Km of alkaline phosphatase acting on p- nitrophenylphosphate in both ELISA and when the enzyme is free in solution.The experiments were carried out at 25C and at a pH of 9.8.The result was Km= 0.246 for the immobilised enzyme and 0.505 for the free solution. I would be grateful for any comments on these results. It means that there was greater affinity of the enzyme for the substrate when it was immobilised, which is not what we expected. Isla Browning Diagnostics and Molecular Biology Section SASA Edinburgh Tel From owner-diagnostics@net.bio.net Thu Aug 31 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test ignore Date: 1 Sep 1995 09:23:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk