From owner-diagnostics@net.bio.net Mon Sep 04 23:00:00 1995 Path: biosci!NIS.NET!empire From: empire@NIS.NET (empire) Newsgroups: bionet.diagnostics Subject: DNA fingerprinting of TB Date: 5 Sep 1995 14:36:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To all that can help, I am looking for information that can be sent to be on utilizing PCR DNA finger printing to compare strains of TB and determine where specific srains came from. All the information that can be sent will be appreciated elementry and advanced material is what i require. thanks to all those that can help Hal From owner-diagnostics@net.bio.net Tue Sep 05 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Udo Reischl" Newsgroups: bionet.diagnostics Subject: Re: DNA fingerprinting of TB Date: 6 Sep 1995 12:09:01 +0100 Organization: Medizin. Mikrobiologie und Hygiene Lines: 48 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <42jvgd$rjp@mserv1.dl.ac.uk> X-Confirm-Reading-To: "Udo Reischl" Original-To: diagnost@dl.ac.uk X-Pmrqc: 1 >To all that can help, >I am looking for information that can be sent to be on utilizing >PCR DNA finger printing to compare strains of TB and determine where >specific srains came from. All the information that can be sent will be >appreciated elementry and advanced material is what i require. >thanks to all those that can help >Hal Dear Hal, Next to routine TB diagnosis (microscopy, BACTEC and other conventional culture/biochemical typing procedures, PCR, NASBA) we are currently evaluating a number of RFLP protocols for epidemiological purposes. Here are some of the corresponding publications and principles: (van Embden et al., J. Clin. Microbiol. 31, 406-409, 1993): PvuII cleavage of genomic DNA and subsequent hybridization with an IS 6110 specific probe. (Ross and Dywer, J. Clin. Microbiol. 31, 329-334, 1993): Amplification of DNA between clusters of IS6110 on the genome. (Friedman et al., J. Clin. Microbiol. 33, 1383-1384, 1995): Amplification of DNA between polymorphic GC-rich repetitive sequences and IS6110 elements. However, this advanced protocol is our favourite: (W. Haas et al., Mixed-linker polymerase chain reaction..., J. Clin. Microbiol. 31, 1293-1298, 1993): PvuII cleavage of traces of genomic DNA and subsequent amplification of IS6110 containing fragments. Upon request I will send you more detailed information on the advantages/ disadvantages associated with the individual strategies. All the best, Udo. ---------------------------------------------- Udo Reischl, Ph.D. Institute for Medical Microbiology and Hygiene at the University of Regensburg Franz-Josef-Strauss Allee 11 D-93053 Regensburg Phone: ++49-941-944-6451 FAX: ++49-941-944-6402 E-mail: Udo.Reischl@klinik.uni-regensburg.de ------------------------------------------------ From owner-diagnostics@net.bio.net Wed Sep 06 23:00:00 1995 Path: biosci!UNIXG.UBC.CA!xiangl From: xiangl@UNIXG.UBC.CA (Xiang Li) Newsgroups: bionet.diagnostics Subject: Sign on. Date: 7 Sep 1995 08:49:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Manager, Could you please subscribe me in to the list. Thanks a lot. Xiang Li West-East Centre for Microbial Diversity University of British Columbia BC, Canada From owner-diagnostics@net.bio.net Thu Sep 07 23:00:00 1995 Path: biosci!TACAPE.BF.EPM.BR!sang From: sang@TACAPE.BF.EPM.BR Newsgroups: bionet.diagnostics Subject: patternity? Date: 8 Sep 1995 04:31:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509081018.AA01263@tacape.bf.epm.br> NNTP-Posting-Host: net.bio.net Hi Collegues, Can anyone help me explaning the difference of using PCR ou RFLP to do patternity test? If you can send me any reference regarding it I will appreciate it very much. Han sang@tacape.bf.epm.br From owner-diagnostics@net.bio.net Thu Sep 07 23:00:00 1995 Path: biosci!aol.com!DjayR From: DjayR@aol.com Newsgroups: bionet.diagnostics Subject: Genetic Testing Date: 8 Sep 1995 13:14:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <950908161203_94521602@mail06.mail.aol.com> NNTP-Posting-Host: net.bio.net Does anyone know where one can obtain information about the current state of the PCR/genetic testing market? Like who is doing it and what revenues are being generated? Thanks J Rothstein,Ph.D. Thomas Jefferson University rothstei@hendrix.jci.tju.edu From owner-diagnostics@net.bio.net Thu Sep 07 23:00:00 1995 Path: biosci!aol.com!MHughes77 From: MHughes77@aol.com Newsgroups: bionet.diagnostics Subject: Seeking antibodies specific for Gardnerella vaginalis Date: 8 Sep 1995 11:14:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <950908141210_14206998@mail04.mail.aol.com> NNTP-Posting-Host: net.bio.net To DIAGNOSTICS newsgroup members: I am developing antigen tests for sexually transmitted diseases, and am looking for a source for antibodies against Gardnerella vaginalis. A commercial supplier would be the most desirable. Thanks, Mark Hughes Empyrean Diagnostics, Inc. 2761 Marine Way Mountain View, CA 94043 USA Tel: 415-960-0516 Fax: 415-960-0515 mhughes77@aol.com From owner-diagnostics@net.bio.net Thu Sep 07 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.mel.aone.net.au!inferno.mpx.com.au!news.unimelb.EDU.AU!news From: Denton Newsgroups: bionet.diagnostics Subject: Cytogentetics Position Vacancy Date: 4 Sep 1995 06:50:49 GMT Organization: The University of Melbourne Lines: 21 Message-ID: <42e7k9$14i9@news.unimelb.EDU.AU> NNTP-Posting-Host: ariel.ucs.unimelb.edu.au Mime-Version: 1.0 Content-Type: text/plain Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) POSITION VACANT CYTOGENETICS There is a position currently available for a an English speaking cytogeneticist with some experience at: Prophase Pathology, Melbourne Australia. For details contact: Ed Krumins 03 9416 2418 after 11th September 1995. From owner-diagnostics@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!newsxfer.itd.umich.edu!news.eecs.umich.edu!newshost.marcam.com!uunet!in1.uu.net!news.sprintlink.net!howland.reston.ans.net!newsjunkie.ans.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: hansgoerl@aol.com (HANS GOERL) Newsgroups: bionet.diagnostics Subject: Re: Experiences with lab standards for genetic testing? Date: 10 Sep 1995 00:05:28 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 11 Sender: root@newsbf02.news.aol.com Message-ID: <42to68$2an@newsbf02.news.aol.com> References: <41lffn$ect@cronkite.ocis.temple.edu> Reply-To: hansgoerl@aol.com (HANS GOERL) NNTP-Posting-Host: newsbf02.mail.aol.com An NIH task force headed by Dr. Holtzmann at Johns Hopkins Sch of P.H. is presently considering all of the issues of lab standards in genetic testing. They are also considering whose responsibility it should be to ensure that probands receive proper genetic counseling. A copy of the report of their first meeting is available on e-mail request from the undersigned. Hans Goerl genethics@delphi.com From owner-diagnostics@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!agate!library.ucla.edu!unixg.ubc.ca!news.bc.net!news.uoregon.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!news.sprintlink.net!EU.net!sun4nl!news.euro.net!usenet From: "Andre W. Schram" Newsgroups: bionet.diagnostics Subject: ANNOUNCEMENT: PROTEIN BIOTECHNOLOGY COURSE Date: 10 Sep 1995 16:38:49 GMT Organization: BioUpdate Foundation Lines: 26 Message-ID: <42v4ap$1l5@news.euro.net> NNTP-Posting-Host: dial6.nedernet.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news://news.nedernet.nl/bionet.diagnostics Protein Biotechnology Course held in the Netherlands October 30 - November 3 1995 Organised by the BioUpdate Foundation, the Netherlands Studies of the biochemistry of disease, coupled with advances in recombinant DNA technology have led to massive commercial interest in the production of highly purified, stable proteins. Apart from protein therapeutics, expanding markets also exist in diagnostics, industrial enzymes as biocatalysts, veterinary products and molecular biology reagents. This protein biotechnology course addresses developments in protein technology from protein extraction, through the various protein purification stages, to their presentation as stable products. The respective merits of various methods for protein characterisation and quality control are compared, issues of protein stability are treated in detail, and topics of current interest, such as protein engineering and protein glycosylation are reviewed. More information available on the BioUpdate homepage: http://www.nedernet.nl/bioupdate/index.html From owner-diagnostics@net.bio.net Sat Sep 09 23:00:00 1995 Path: biosci!SHANI.NET!ffish From: ffish@SHANI.NET (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Drying retardants Date: 10 Sep 1995 14:22:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <01HUHWMY0YWY004WCP@INVERMAY.CRI.NZ> NNTP-Posting-Host: net.bio.net On 24 Aug 1995 HartA@agresearch.cri.nz wrote: > > Suppose I have a thin aqueous film containing enzyme(s). Can anyone suggest > a suitable additive(s) which will prevent the film from drying out, without > affecting the activity of the enzyme? > > Thanks, > > Alan Hart, > AgResearch Grasslands. > > Try glycerol or PVP. Falk Fish, Tel Aviv. From owner-diagnostics@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!howland.reston.ans.net!news.sprintlink.net!in2.uu.net!news.deltanet.com!usenet From: amazing@deltanet.com (Ray Robinson) Newsgroups: bionet.diagnostics Subject: computer vs human malfunctions: http://www..... Date: 11 Sep 1995 00:15:36 GMT Organization: Delta Internet Services, Anaheim, CA Lines: 13 Message-ID: <42vv38$44t@news2.deltanet.com> NNTP-Posting-Host: ana2071.deltanet.com X-Newsreader: WinVN 0.92.6+ Are the principles of Doctors the same as those of Computer Repairmen? I know of one example: both are concerned with the queston of WHEN did something unusual happen. Are there any others? Also enjoy some fun new types of Chess puzzles, Checkers, GO; and those master deceivers: Magicians! Please check-out/enjoy my homepage at: http://www.deltanet.com/users/amazing/ Thanks in advance for the visit! From owner-diagnostics@net.bio.net Sun Sep 10 23:00:00 1995 Path: biosci!CSHL.ORG!boyce From: boyce@CSHL.ORG (Joan Boyce) Newsgroups: bionet.diagnostics Subject: New WWW resource - an online buyer's guide for research products and services Date: 11 Sep 1995 11:52:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509111848.AA02522@phage.cshl.org> NNTP-Posting-Host: net.bio.net Cold Spring Harbor Lab Press introduces BIOSUPPLYNET-An online directory of biotech research products and services. This new WWW site offers free searching in the CSH Lab Manual Source Book database of 15,000 laboratory products and 1,400 suppliers. BIOSUPPLYNET features "Interactive Product Listings" (IPLs), which provide users with instant access to details on many of the products contained within its extensive database. BIOSUPPLYNET is updated weekly and provides: 1.) Information on new products and special offers 2.) Access to 15,000 laboratory product listings from over 1,400 suppliers 3.) An opportunity for users to share expertise through product user groups 4.) Immediate access to suppliers via email for technical queries and ordering information 5.) Links to more than 100 biology-relevant Web servers BSN is accessible at: http://www.biosupplynet.com/bsn/ A FREE copy of the CSH Lab Manual Source Book can be obtained through Cold Spring Harbor Web Site, CLIO, http://www.cshl.org/ Hope you all find it useful! Joan Boyce boyce@cshl.org From owner-diagnostics@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!SIAL.COM!patersot From: patersot@SIAL.COM (Tom Paterson) Newsgroups: bionet.diagnostics Subject: surfactant fall-out Date: 13 Sep 1995 13:12:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello I would appreciate any help with the following problem: I have a friend who has a solution containing 0.19 M NaOH and 0.045M SDS (Sodium Dodecyl Sulfate), pH approx. 13 . How does my friend keep the SDS in solution @ temp. less than 19 degrees C? Thank you in advance _______________________________________________________________________ Tom Paterson | Sigma Diagnostics Clinical Marketing | 545 South Ewing, St Louis, MO 63103 patersot@sial.com | (314)771-5765 x2084 From owner-diagnostics@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lille1.fr!u-strasbg.fr!ciril.fr!piaf58.spieao.u-nancy.fr!user From: michel@spieao.u-nancy.fr (Christophe Michel) Newsgroups: bionet.diagnostics Subject: heart failure & skeletal muscle Date: Wed, 13 Sep 1995 17:28:32 +0200 Organization: CIRIL, Nancy, France Lines: 23 Message-ID: NNTP-Posting-Host: piaf58.spieao.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit hello ------ I would like to discuss with persons interested in skeletal muscle repercussions of heart failure. clinical cases or experimental models hoping to hearing from you soon. Christophe Michel Laboratoire de chirurgie experimentale Faculte de medecine de nancy B.P. 184 9 avenue de la foret de haye 54505 vandoeuvre les nancy cedex e-mail:michel@spieao.u-nancy.fr From owner-diagnostics@net.bio.net Tue Sep 12 23:00:00 1995 Path: biosci!dundee.ac.uk!K.C.BREEN From: K.C.BREEN@dundee.ac.uk (Kieran Breen) Newsgroups: bionet.diagnostics Subject: Lectureship Date: 13 Sep 1995 16:21:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <31491C0951@ninewells.dundee.ac.uk> NNTP-Posting-Host: net.bio.net Dept. of Pharmacology and Clinical Pharmacology University of Dundee LECTURESHIP IN PHARMACOLOGY Applications are invited for a non-clinical Lectureship within the Pharmacology Section of the Deptartment of Pharmacology and Clinical Pharmacology. The Department has consistently been highly rated for research and this has been greatly strengthened by the recent development of a Neurosciences Institute within the Department. Applicants from any area of Pharmacological research will be considered, although an ability to complement and interact with existing research groups would be an advantage. The appointment is for 3 years in the first instance and will be on the Lecturer A salary scale (#14,317-19,848). Dundee has an international reputation for research in the life sciences and the newly refurbished laboratories and offices of the Department are housed within the Medical School in Ninewells Hospital which is situated on the Tay Estuary. Informal enquiries are welcomed at NEURO@PHARMACOLOGY.DUNDEE.AC.UK. A CV (3 copies) complete with the names and addresses of 3 referees, should be sent to Personnel services, University of Dundee, Dundee DD1 4HN, Scotland, UK (tel +44-1382-344015) from whom further particulars are available. From owner-diagnostics@net.bio.net Wed Sep 13 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!ahung From: ahung@ASRR.ARSUSDA.GOV Newsgroups: bionet.diagnostics Subject: Picornavirus Date: 14 Sep 1995 08:13:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues: Currently I am trying to propagate bee picornavirus using tissue culture cells. So far the wasp cell culture does not appear to be able to grow this virus. Has anyone had any luck with this type of viurs in any other type of cell culture systems? Thank you in advance for this information. Akey Hung Bee Research Laboratory USDA, Beltsville, MD From owner-diagnostics@net.bio.net Wed Sep 13 23:00:00 1995 Path: biosci!MC.DUKE.EDU!JOHNS053 From: JOHNS053@MC.DUKE.EDU Newsgroups: bionet.diagnostics Subject: subscribe Date: 14 Sep 1995 14:22:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HV9VN6RYEQ001QVT@mc.duke.edu> NNTP-Posting-Host: net.bio.net Please add me to your group. Stewart P. Johnson Department of Pathology Duke University Medical Center From owner-diagnostics@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!tank.news.pipex.net!pipex!warwick!news.ncl.ac.uk!usenet From: Roger Hayes Newsgroups: bionet.diagnostics Subject: alfentanyl Date: 15 Sep 1995 14:01:07 GMT Organization: University of Newcastle Lines: 3 Message-ID: <43c0v3$31l@whitbeck.ncl.ac.uk> NNTP-Posting-Host: anaes18.ncl.ac.uk Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Can anyone give me some info. on RIA assay of alfentanyl and it's primary metabolites with particular ref. to supply of antibodies.Thanks,Roger From owner-diagnostics@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!WADSWORTH.ORG!jack.woodall From: jack.woodall@WADSWORTH.ORG Newsgroups: bionet.diagnostics Subject: Diphtheria tests: RFI (fwd) Date: 15 Sep 1995 06:59:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <199509151356.JAA06942@wadsworth.ph.albany.edu> NNTP-Posting-Host: net.bio.net >Date: Fri, 15 Sep 1995 08:26:18 +0100 >From: "Alenka Kraigher, IVZ, +61 1323 245" >Subject: Analysing Diphteria immunity >Diphtheria outbreak in Eastern Europe shows that the risk of diphtheria is >far from having disappeared. >For this reason, in the National Institute for Public Health Slovenia we >plan to check diphtheria immunity levels in the adult population before >modification the diphtheria immunisation strategy to strengthen population >immunity as a whole. > >Among serological methods we chose indirect ELISA test (toxin-binding >inhibition test, ToBI test) and in vitro neutralisation test on VERO cells. >We found recommendation for those methods in WHO publications, but we could >not find out where the products for executing these tests are produced. > >May I ask if somebody could help us with information where preferably in >Europe we could get products that are necessary for these tests. >The products that we need for ToBI test are: diphtheria toxin, equine >diphtheria antitoxin and peroxidase-labelled sheep antidiphtheria IgG. >We got diphtheria reference serum from Statens Seruminstitut Copenhagen. >Are there any kits for ToBI available? For in vitro neutralisation test we >need: diphtheria toxin (purified) and horse hiperimmune diphtheria antitoxin, >calibrated against WHO standard. > >I will appreciate very much the information. > >Dr. Alenka Kraigher > PLEASE REPLY DIRECTLY TO: -- Jack Woodall, Ph.D., ProMED List Moderator, New York State Dept.of Health, Albany NY, USA e-mail: jack.woodall@wadsworth.org From owner-diagnostics@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!usc!sdd.hp.com!news1.best.com!news3.net99.net!news.cais.net!news.vbc.net!news.vbc.net!SoNet!news From: Roger@gemini.co.uk (Roger Moreton) Newsgroups: bionet.diagnostics Subject: Re: New WWW resource - an online buyer's guide for research products and services Date: Thu, 14 Sep 1995 22:53:13 GMT Organization: Gemini Scientific Communications Ltd Lines: 9 Message-ID: <43abs1$23g@alpha.aladdin.co.uk> References: <9509111848.AA02522@phage.cshl.org> NNTP-Posting-Host: gemini.aladdin.co.uk X-Newsreader: Forte Agent .99a.107 boyce@CSHL.ORG (Joan Boyce) wrote: Your address for comments appears not to be available I would be grateful; if you would contact me as I would wish to correct some information in your pages Gemini Scientific Communications Ltd http://www.gemini.co.uk/biopages PO BOX 138 Southampton UK SO15 0ZD From owner-diagnostics@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!COMPUSERVE.COM!100416.735 From: 100416.735@COMPUSERVE.COM (The Diagnostics Club) Newsgroups: bionet.diagnostics Subject: Diagnostics Club Meeting Date: 15 Sep 1995 11:33:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <950915182856_100416.735_BHG101-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net TARGETED RESEARCH - ASSESSMENT AND STRATEGY FOR BIOMEDICAL DIAGNOSTICS Joint meeting of The Association of Clinical Biochemists, the British In Vitro Diagnostics Association, and The Diagnostics Club Date: Tuesday 28th November 1995 Time: 10.00 to 16.30hrs Venue: Postgraduate Medical Centre, Queen Elizabeth Hospital, Birmingham, UK Cost: UKpounds sterling 40 (including VAT) PROGRAMME NHS R&D and theMEDLINK programme Dr Peter Greenaway, Department of Health. Health technology assessment methodology and Case Study Dr Ala Szczepura, Centre for Healthcare Service Study, Warwick Business School. Implementation of the Foresight Programme, Prof Mark Ferguson, University of Manchester. Evidence based healthcare- systematic reviews of outcome measures, Dr Andrew Moore, Churchill Hospital, Oxford. The genetics of common diseases - implications for the future, Dr Eric Sidebottom, Research consultant to the Department of Health. Technology needs for the next decade, Prof Chris Price, London Hospital Medical College. For further information contact: Dr Valerie Owen tel: +44 1908 647417, fax: +44 1908 271612, email 100416.735@compuserve.com From owner-diagnostics@net.bio.net Thu Sep 14 23:00:00 1995 Path: biosci!greb.ulaval.ca!Christian.Mouton From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) Newsgroups: bionet.diagnostics Subject: Immunomagnetic PCR\ DNA Probe\ Porphyromonas gingivalis Date: 15 Sep 1995 13:59:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <9509152057.AA21911@hermes.ulaval.ca> NNTP-Posting-Host: net.bio.net The following is the summary of a paper which has been accepted for publication by J. Clin. Microbiol. Of particular interest is the fact that the DNA probe and the PCR protocol specific for Porphyromonas gingivalis were derived from the sequence of a random amplified polymorphic DNA (RAPD) species-specific fragment. We are currently developing similar procedures for the diagnosis of other fastidious gram negative anaerobes in mixed infections. Immunomagnetic PCR and a DNA Probe for the Detection and Identification of Porphyromonas gingivalis. Riad M. Benkirane, Emmanuelle Guillot, and Christian Mouton The aim of this study that we describe was to combine an immunomagnetic separation and a polymerase chain reaction followed by dot blot hybridization with a DNA probe for the detection and identification of Porphyromonas gingivalis. Immunomagnetic particles were coated with monoclonal antibody specific for P. gingivalis and incubated with a suspension of seven oral bacterial species spiked with various dilutions of P. gingivalis. Beads with their load of bound bacteria were boiled in water, and the target DNA in the supernatant was amplified with a primer pair to generate a 593-bp PCR fragment specific for P. gingivalis. Finally, the product of amplification was detected by dot-blot hybridization with a digoxigenin-labeled 593-bp probe. The detection limit was determined at 100 bacterial cells/ml. The immunomagnetic-PCR/DNA probe procedure described here should be useful for the rapid, specific and sensitive detection and identification of P. gingivalis in clinical samples. -----------------------------Christian Mouton----------------------------- Groupe de Recherche en Écologie Buccale tél. (418)656-5872 Faculté de médecine dentaire fax. (418)656-2861 Université Laval Email: Christian.Mouton@greb.ulaval.ca Québec (Québec) G1K 7P4 CANADA From owner-diagnostics@net.bio.net Sat Sep 16 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!uunet!in1.uu.net!bull.hkstar.net!b2!alo From: alo@b2.hkstar.com (Lo Wing Ip Anthony) Newsgroups: bionet.diagnostics Subject: Molecular Diagnostic Kit for TB Date: 17 Sep 1995 03:37:59 GMT Organization: Hong Kong Star Internet Ltd. Lines: 13 Message-ID: <43g56n$jn4@bull.hkstar.net> NNTP-Posting-Host: b2.hkstar.com X-Newsreader: TIN [version 1.2 PL2] Hi, I am looking for a PCR-based diagnostic kit for the quick identification of Mycobacteria tuberculosis, basically on miscellaneous body fluid including CSF, PD fluid, etc. If the cost is reasonable, I would also like to incorporate the test into sputum analysis. Thank you for your attention Anthony Lo Chemical Pathology Princess Margaret Hospital Hong Kong From owner-diagnostics@net.bio.net Sun Sep 17 23:00:00 1995 Path: biosci!BEPC2.IHEP.AC.CN!wangyq From: wangyq@BEPC2.IHEP.AC.CN Newsgroups: bionet.diagnostics Subject: help Date: 18 Sep 1995 08:34:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <009969A6.CEAD93A0.94@bepc2.ihep.ac.cn> NNTP-Posting-Host: net.bio.net Dear Netters: I want to find mumbranes and glass fibers used on manufacture of fast test stripes for pregnancy and hepatitis. Any help would be very appreciated. Thank you. Wang Yuquan Beijing Cons Bio-Tech From owner-diagnostics@net.bio.net Sun Sep 17 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!EU.net!Belgium.EU.net!chaos.kulnet.kuleuven.ac.be!news.belnet.be!news.vub.ac.be!usenet From: GREETINGS FROM CYBERSPACE Newsgroups: bionet.diagnostics,bionet.drosophila Subject: Re: Hello, talk to me Date: 16 Sep 1995 09:23:42 GMT Organization: Brussels Free Universities VUB/ULB Lines: 2 Message-ID: <43e52u$fta@rc1.vub.ac.be> References: <094424Z11081995@anon.penet.fi> <40ghjj$c4a@panix2.panix.com> <419s83$bnq@dux.dundee.ac.uk> NNTP-Posting-Host: hwepc25.vub.ac.be. Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: kappleyard@ninewells.dundee.ac.uk Xref: biosci bionet.diagnostics:303 bionet.drosophila:1391 Hello my name is Michel From owner-diagnostics@net.bio.net Sun Sep 17 23:00:00 1995 Path: biosci!UIDAHO.EDU!magri892 From: magri892@UIDAHO.EDU (Vincent J Magrini) Newsgroups: bionet.diagnostics Subject: Spinner cultures Date: 18 Sep 1995 13:34:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi all... I am curious on the advantages of spinner cultures or roller bottles over tissue culture flasks to propagate picorna viruses in vero cells. For example, are the viral yields significantly greater compared to tissue culture flasks? Also, can I use the adherent vero cells in a spinner or roller system? Gee, any advice is greatly appreciated.... Thanks in advance, sincerely, Vincent J. Magrini From owner-diagnostics@net.bio.net Mon Sep 18 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!ee.und.ac.za!iafrica.com!ticsa.com!cstatd.cstat.co.za!slipper101144.iafrica.com!cranks From: cranks@iafrica.com (Neal Crankshaw) Newsgroups: bionet.diagnostics Subject: Request explanation Date: Tue, 19 Sep 1995 20:30:26 Organization: Internet Africa Lines: 7 Distribution: world Message-ID: NNTP-Posting-Host: slipper101144.iafrica.com X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In a blood analysis report, to what does the "Prostate Antigen Level" refer? I would appreciate a fair level of detail as to what it means. TIA Neal cranks@iafrica.com From owner-diagnostics@net.bio.net Thu Sep 21 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!mcshub!informer1.cis.McMaster.CA!mcmail.CIS.McMaster.CA!not-for-mail From: g8919516@mcmail.cis.McMaster.CA (C. Muir) Newsgroups: bionet.diagnostics Subject: mouse blood ELISA Date: 22 Sep 1995 12:14:33 -0400 Organization: McMaster University, Hamilton, Ontario, Canada. Lines: 4 Message-ID: <43und9$9av@mcmail.CIS.McMaster.CA> NNTP-Posting-Host: mcmail.cis.mcmaster.ca I am looking for a pharmaceutical manufacturer/distributor who produces an ELISA assay specifically for mouse 17-beta-estradiol, progesterone or prolactin. If you know of one please e-mail me (Cam) a phone number or address at g8919516@mcmaster.ca thanks. From owner-diagnostics@net.bio.net Fri Sep 22 23:00:00 1995 Path: biosci!daresbury!nntp-trd.UNINETT.no!Norway.EU.net!EU.net!news.sprintlink.net!in2.uu.net!news1.digital.com!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!unixg.ubc.ca!news From: "Gary C. Donaldson" Newsgroups: bionet.diagnostics Subject: Re: Molecular Diagnostic Kit for TB Date: 23 Sep 1995 08:48:48 GMT Organization: University of British Columbia Lines: 16 Message-ID: <440hlg$muh@nntp.ucs.ubc.ca> References: <43g56n$jn4@bull.hkstar.net> NNTP-Posting-Host: port61.annex2.net.ubc.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) I believe that Roche Diagnostics (a division of the Swiss pharmaceutical giant Hoffman LaRoche) sells a PCR diagnostic kit for M. tuberculosis. Hope this helps. --------------------------------------------------------------------------------------------- Gary C. Donaldson Dept. of Plant Science, University of B.C. E-mail: garyd@unixg.ubc.ca donaldson@bcr.bc.ca 72700.2227@compuserve.com ---------------------------------------------------------------------------------------------- There are three kinds of lies. Lies, damn lies, and statistics. -Mark Twain ---------------------------------------------------------------------------------------------- From owner-diagnostics@net.bio.net Sun Sep 24 23:00:00 1995 Path: biosci!roche.com!GREG.OGLE From: GREG.OGLE@roche.com Newsgroups: bionet.diagnostics Subject: MTB Diagnostics Date: 25 Sep 1995 14:51:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HVP9VA6BKMHW9VLE@rnuc01.nut.roche.com> NNTP-Posting-Host: net.bio.net --Boundary (ID iCALNjH9OcuednBU8yHBnQ) Content-type: TEXT/PLAIN; CHARSET=ISO-8859-1 Yes...Yes it is true. RDS in NJ sells or is close to selling the Amplicor Mtb kit for RESEARCH ONLY. If you were at ICAAC you saw the Amplicor COBAS which will also incorporate Mtb in the near future. Greg Ogle Roche Molecular Systems Alameda CA --Boundary (ID iCALNjH9OcuednBU8yHBnQ) Content-type: TEXT/PLAIN; CHARSET=ISO-8859-1; NAME=untitled.TXT I believe that Roche Diagnostics (a division of the Swiss pharmaceutical giant Hoffman LaRoche) sells a PCR diagnostic kit for M. tuberculosis. Hope this helps. --------------------------------------------------------------------------------------------- Gary C. Donaldson Dept. of Plant Science, University of B.C. E-mail: garyd@unixg.ubc.ca donaldson@bcr.bc.ca 72700.2227@compuserve.com ---------------------------------------------------------------------------------------------- There are three kinds of lies. Lies, damn lies, and statistics. -Mark Twain ---------------------------------------------------------------------------------------------- --Boundary (ID iCALNjH9OcuednBU8yHBnQ)-- From owner-diagnostics@net.bio.net Mon Sep 25 23:00:00 1995 Path: biosci!roche.com!GREG.OGLE From: GREG.OGLE@roche.com Newsgroups: bionet.diagnostics Subject: Sputum samples Date: 26 Sep 1995 10:22:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HVQEJEPZCAHW9W7C@rnuc01.nut.roche.com> Greetings all! We are in dire need of some sputum samples and would be much obliged if anyone could help out. If you have some names of contacts or if you can provide the sputum yourself (neg and positive, raw and liquified) please get in contact with me. We can discuss it further and get an MTA in the works. Please E-mail me at: greg.ogle@roche.com or call in California at (510) 814-2936 Thank you for your help. -Greg Ogle Roche Molecular Systems Alameda, CA From owner-diagnostics@net.bio.net Tue Sep 26 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!sol.ctr.columbia.edu!howland.reston.ans.net!swrinde!sgigate.sgi.com!news1.best.com!shellx.best.com!usenet From: Newsgroups: bionet.immunology,bionet.diagnostics,bionet.microbiology,bionet.parasitology Subject: Enterocytozoon bieneusi specific antibodies? Date: 27 Sep 1995 21:10:04 GMT Organization: Alexon Biomedical Lines: 14 Message-ID: <44cejc$n0c@shellx.best.com> NNTP-Posting-Host: alexon.vip.best.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: matt@alexon.com X-URL: news:bionet.immunology Xref: biosci bionet.immunology:5624 bionet.diagnostics:311 bionet.microbiology:3409 bionet.parasitology:1060 Hello, I am looking for a source(s) of antibodies specific for Enterocytozoon bieneusi. I would be testing the feasibility of their use in commercial diagnostic immunoassays. Both polyclonal (any host species) and monoclonal antibodies are desired. Any helpful leads would be greatly appreciated. Matt Boisen Research Associate Alexon Biomedical matt@alexon.com