From owner-diagnostics@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Missing posts Date: 4 Oct 1995 13:04:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Following the succesful vote on full newsgroup status for bionet.diagnostics, I retained an e-mail subscription as well as read= ing it as a newsgroup. Recently I have noticed that some posts which I receive by e-mail have not appeared on the version of diagnostics I get = on my newsreader. In particular, someone posted a very interesting abstract = of a recent publication which I deleted from my e-mail in-tray in the = expectation that I would have the newsreader version. In addition, I posted a version of this message via my newsreader some = days ago. Although it has appeared there, it has not appeared as an e-mai= l message! Has anyone else noticed this discrepancy or is it a problem specific to = my end of things? Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Wed Oct 04 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!plug.news.pipex.net!pipex!dish.news.pipex.net!pipex!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: vigfrid.ness@veths.no (Vigfrid Ness) Newsgroups: bionet.diagnostics Subject: Protozoa Date: 5 Oct 1995 14:47:50 +0100 Lines: 8 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <450nm6$bj3@mserv1.dl.ac.uk> X-Sender: nmhness@samson.veths.no Original-To: diagnost@dl.ac.uk Hi For bacteria one can order strains from ATCC and NCTC etc. My question is does there excist similar collections for protozoa???? And if so how do I get in touch with them -address, fax etc. Vigfrid N=E6ss From owner-diagnostics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: Protozoa Date: 6 Oct 1995 03:26:13 -0700 Organization: Scottish Agricultural College Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510061020.DAA01770@net.bio.net> NNTP-Posting-Host: net.bio.net > Date: Thu, 05 Oct 95 20:35:23 > From: > Subject: Protozoa > To: mx%"diagnost@net.bio.net" > Cc: > Return-Path: > Received: from castle.ed.ac.uk (castle) by SAC001.ed.sac.ac.uk (MX V4.1 AXP) > with SMTP; Thu, 05 Oct 1995 20:35:22 GMT > Received: from net.bio.net by castle.ed.ac.uk id aa26034; 5 Oct 95 20:33 BST > Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id KAA01272 for > diagnost-list; Thu, 5 Oct 1995 10:43:47 -0700 > Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id KAA01264 for > diagnost-arpanet; Thu, 5 Oct 1995 10:43:44 -0700 > To: diagnost@net.bio.net > From: Vigfrid Ness > Subject: Protozoa > Date: 5 Oct 1995 14:47:50 +0100 > Sender: lpddist@mserv1.dl.ac.uk > Message-ID: <450nm6$bj3@mserv1.dl.ac.uk> > X-Sender: nmhness@samson.veths.no > Original-To: diagnost@dl.ac.uk > > Hi > > For bacteria one can order strains from ATCC and NCTC etc. > My question is does there excist similar collections for protozoa???? > And if so how do I get in touch with them -address, fax etc. > > Vigfrid N=E6ss > > > > I have no USA address, but for Europe you could try the Information Centre for European Culture Collections, Braunschweig, Germany, tel 0531 61 8715/22 Rob Harling From owner-diagnostics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!IPO.AGRO.NL!H.HUTTINGA From: H.HUTTINGA@IPO.AGRO.NL Newsgroups: bionet.diagnostics Subject: (none) Date: 6 Oct 1995 02:23:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HW45N6IPVI0008BR@IPO.AGRO.NL> NNTP-Posting-Host: net.bio.net This is to inform you that October 10th, 1995 my E-mail address will be changed from H.Huttinga@ipo.agro.nl to H.Huttinga@ipo.DLO.nl. At the same time almost all telephone numbers in the Netherlands will be changed. Kind regards, H. Huttinga DLO Research Institute for Plant Protection (IPO-DLO) Binnenhaven 5, P.O.Box 9060, 6700 GW Wageningen, the Netherlands After October 10, 1995: Tel.: ** 31 317 476249 Fax.: ** 31 317 410113 E-mail: H.Huttinga@IPO.DLO.NL From owner-diagnostics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!aol.com!EDiagnos From: EDiagnos@aol.com Newsgroups: bionet.diagnostics Subject: Development of Hepatitis A Rapid Tests Date: 6 Oct 1995 10:33:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <951006133147_38017844@mail06.mail.aol.com> NNTP-Posting-Host: net.bio.net Diagnostics Newsgroup Members: We are working on the development of a Hepatitis A rapid ELISA kit. Hepatitis A antigens from two sources have been tested, but results have not been satisfactory. We would like to test Hepatitis A antigens from other sources. Hepatitis A patient samples are also needed for the study. Any information regarding sources of Hepatitis A antigen and patient samples will be highly appreciated. Thanks, Tsai-Hsia Hong ediagnos@aol.com ************************************************ Tsai-Hsia Hong, Ph.D. Assistant Director, Immunology Division 2761 Marine Way Mountain View, CA 94043 USA Phone 415-960-0516 Fax 415-691-1578 ************************************************ From owner-diagnostics@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!fnnews.fnal.gov!nntp-server.caltech.edu!mac1553.bio.caltech.edu!user From: grovesa@starbase1.caltech.edu (Andrew K. Groves) Newsgroups: bionet.cellbiol,bionet.diagnostics,bionet.neuroscience,bionet.molbio.methds-reagnts Subject: Non-Radioactive In Situ Protocols Date: 6 Oct 1995 20:46:21 GMT Organization: California Institute of Technology Lines: 18 Message-ID: NNTP-Posting-Host: mac1553.bio.caltech.edu Xref: biosci bionet.cellbiol:3099 bionet.diagnostics:318 bionet.neuroscience:10597 bionet.molbio.methds-reagnts:34556 We have set up a Web Page for our lab's in situ hybridisation protocols. These protocols describe the use of non-radioactive probes for in situ hybridisation on tissue sections, cultured cells and whole mounts. The Web page can be found at: http://www.cco.caltech.edu/~mercer/htmls/Big_In_Situ.html which is part of Eric Mercer's comprehensive Mouse and Rat Resource page at: http://www.cco.caltech.edu/~mercer/htmls/rodent_page.html -- Andy Groves Division of Biology, 216-76 California Institute of Technology From owner-diagnostics@net.bio.net Sat Oct 07 23:00:00 1995 Path: biosci!DELTANET.COM!tfoley From: tfoley@DELTANET.COM (Thomas J. Foley) Newsgroups: bionet.diagnostics Subject: Cholera Test Date: 8 Oct 1995 15:39:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello Fellow Netters, Anybody out there who knows of any commercially available diagnostic test kits for the detection of cholera? Thanking you in anticipation. Tom Foley, tfoley@deltanet.com From owner-diagnostics@net.bio.net Sun Oct 08 23:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!msunews!netnews.upenn.edu!cronkite.ocis.temple.edu!astro.ocis.temple.edu!gold From: gold@astro.ocis.temple.edu (Bert Gold) Newsgroups: bionet.molbio.genome-program,bionet.diagnostics Subject: Struewing Article on BRCA1 in Nature Geneticssc Date: 9 Oct 1995 04:56:43 GMT Organization: Temple University, Academic Computer Services Lines: 36 Message-ID: <45aa2c$lf2@cronkite.ocis.temple.edu> NNTP-Posting-Host: astro.ocis.temple.edu Summary: m Keywords: : X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.molbio.genome-program:1529 bionet.diagnostics:322 Hi Netters, Having just finished reading the new Nature Genetics paper by Struewing et al. on the high (1%) frequency of the BRCA1 mutation among Ashkenazi Jews, I would be interested in hearing opinions about the perceived necessity for population screening at this threshold. For those of you that haven't yet seen the paper, it is in: Nature Genetics, 11: 198-200 (1995). I am aware of the concensus conferences cited in the paper which supported screening at this threshold, but that was all theoretical, before we knew that there was a gene that would create this level of anxiety. What are the genetics community's thoughts now? My new email address is bgold@itsa.ucsf.edu, not what you see above. Please feel free to write with your thoughts... It is true that for me this issue strikes home, as indeed, my heritage is that of an Ashkenazi Jew and breast cancer d ovarian cancer have been e in bot own and wife's family. Bert Gold UCSF . From owner-diagnostics@net.bio.net Sun Oct 08 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!daresbury!is.bbsrc.ac.uk!bcc.ac.uk!news From: CIHTFSTR Subject: Re: How to Get Free Cable Premium TV .. LEGIT Sender: news@ucl.ac.uk (Usenet News System) Message-ID: <1995Oct9.163846.28568@ucl.ac.uk> Date: Mon, 9 Oct 1995 16:38:46 GMT To: md@goodnet.com Content-Transfer-Encoding: 7bit Content-Type: text/plain; charset=us-ascii References: Mime-Version: 1.0 X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Organization: lshtm Lines: 15 md@goodnet.com (Mike Denton) wrote: > >Send... > > $1.00 U.S MONEY *** NO COINS *** NO CHECKS *** > inside a sheet of carbon paper or construction paper, > and send a *self-addressed*,*stamped* envelope to the > address above. > > Would you be equally happy with an international reply coupon, or is the purpose just to collect US$ 1.00 notes? It sounds too good to be true... From owner-diagnostics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!COMPUSERVE.COM!100273.165 From: 100273.165@COMPUSERVE.COM (Philip Shaw) Newsgroups: bionet.diagnostics Subject: Missing posts Date: 10 Oct 1995 01:31:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <951010082825_100273.165_EHV89-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net I have wondered if I was still on the list - I get so little these days, I've just been too busy to do something about it. I thought it might be because I use email via compuserve. Philip Biodot Ltd 100273.165@compuserve.com From owner-diagnostics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!usenet.eel.ufl.edu!psgrain!iafrica.com!ticsa.com!cstatd.cstat.co.za!ucthpx!itu1.sun.ac.za!news From: Caz Nahman Newsgroups: bionet.diagnostics Subject: (no subject) Date: 10 Oct 1995 13:19:53 GMT Organization: University of Stellenbosch Lines: 14 Message-ID: <45drtp$qgd@itu1.sun.ac.za> NNTP-Posting-Host: 146.232.30.12 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) > > -------------------------------------------------------------------- > > Newsgroup: bionet.diagnostics > > * Molecular Diagnostic Kit for TB - Lo Wing Ip Anthony (13) > * Re: Hello, talk to me - GREETINGS FROM CYBERSPACE (2) > * Diagnostics Club Meeting - The Diagnostics Club (27) > * Please help with CHONDROBLASTIC OSTEOSARCOMA - begleym (36) > > -------------------------------------------------------------------- > From owner-diagnostics@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!aol.com!PandaGroup From: PandaGroup@aol.com Newsgroups: bionet.diagnostics Subject: Small Chromatography Syringes Date: 10 Oct 1995 09:30:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <951010122833_120454357@emout04.mail.aol.com> NNTP-Posting-Host: net.bio.net Okay all you diagnostics mavens .... it seems to me that in the early to mid-1980s some company came out with small chromatography columns for doing drug or isoenzyme separations (I can't remember which, or both) for clinical use. The column itself was packed in a syringe and after you placed the sample on top of the column, buffer was added and you used the syringe to force the sample through the column ...otherwise, with no hydrostatic head you could wait until you retired for the sample to move through the column. Does anybody else remember these toys and perhaps who manufactured/marketed them? If you do, I'd like to hear from you. Thanks. Ken Krul From owner-diagnostics@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Berit.Gron@veths.no (Berit Gron) Newsgroups: bionet.diagnostics Subject: Re: Antibodies against Hexamita Date: 13 Oct 1995 13:14:11 +0100 Lines: 12 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <45ll6j$aua@mserv1.dl.ac.uk> X-Sender: nmhbgron@samson.veths.no Original-To: diagnost@dl.ac.uk Hello, I am loocking for antibodies against the protozoa Hexamita spp/ Spironucleus spp. I have tried Linscott's and some other catalogues, but so far it has been unsuccessful. Do somebody have a suggestion where I can find these antibodies??? Thanks in advance. Berit Gr=F8n Research Scientist From owner-diagnostics@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!hermes.is.co.za!usenet From: cgrove@lia.co.za (Carel Grove) Newsgroups: bionet.diagnostics Subject: Urgent: Unknown Neurological condition Date: Fri, 13 Oct 1995 19:13:59 GMT Organization: No organisation supplied Lines: 66 Message-ID: <45mdmk$p6p@hermes.is.co.za> NNTP-Posting-Host: p084.infolink.co.za X-Newsreader: Forte Agent .99a.107 I would not normally post something like this but I decided to do it because this is extremely urgent. About an hour ago I was informed about the following situation regarding a friend's daughter. I will only summarize: (She is eight years old and has a family history with Tourette Syndrome). She was playing hockey when she developed acute headache followed by vomiting. This happened very suddenly. She did not get injured physically at all. In other words the headache was not a result of her getting hit on her head or anything like that. She was at first treated for encephalitis with no result. Blood was found in her spinal fluid. X-Rays pointed to a brain tumour in the cerebellum but upon operating no tumour was found, a large blood clot was found instead. (in the cerebellum) She regained consciousness the following day (Monday). Tuesday afternoon she had a series of convulsions (epileptic seizures). After that, a rapid decent into coma. She now has respiratory failure as well as liver failure. Blood platelets so low there is blood seeping out of her mouth and nose. She still has a large amount of Phenobarb in her system, so tests can only be done in 48 hours. She is treated in one of the top clinics in South Africa, but no one seems to know what on earth is happening to her. If anybody knows or has any experience whatsoever with a condition like this, will you please get in contact with me as soon as possible. Any information will be appreciated very very much If possible, please forward this to anyone who might know about this condition. Carel Grove -- Tel: +27 12 667-2291 E-Mail: cgrove@lia.co.za or 100076.1343@compuserve.com From owner-diagnostics@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!rutgers!uwm.edu!chi-news.cic.net!simtel!swidir.switch.ch!in2p3.fr!univ-lyon1.fr!fdn.fr!uunet!in1.uu.net!pipeline!psinntp!psinntp!psinntp!interramp.com!ip39.santa-clara.ca.interramp.com!user From: pp002271@interramp.com (Michael R. Knapp) Newsgroups: bionet.molbio.genome-program,bionet.diagnostics Subject: Re: Struewing Article on BRCA1 in Nature Geneticssc Date: 14 Oct 1995 23:56:19 GMT Organization: Caliper Technologies, Corp. Lines: 25 Message-ID: References: <45aa2c$lf2@cronkite.ocis.temple.edu> NNTP-Posting-Host: ip39.santa-clara.ca.interramp.com Xref: biosci bionet.molbio.genome-program:1548 bionet.diagnostics:329 In article <45aa2c$lf2@cronkite.ocis.temple.edu>, gold@astro.ocis.temple.edu (Bert Gold) wrote: > Having just finished reading the new Nature Genetics paper by > Struewing et al. on the high (1%) frequency of the BRCA1 mutation > among Ashkenazi Jews, I would be interested in hearing opinions > about the perceived necessity for population screening at this > threshold. There would surely be a cost-benefit analysis that could, at a given point in technology time, come up with an answer that takes into account diagnostic testing costs, therapeutic alternatives, life-style adaptation, etc. However, for a while, testing decisions should probably remain personal ones given the emotional implications, inadequate prognostic data, and lack of a broad therapeutic arsenal for most genetic variants that cause disease. If I was at risk for a particular mutation, I would want to know and would inform myself systematically of research progress. However, for most people, having access to an informed health care community that can intelligently present options to patients in a rapidly changing knowledge environment will be the only way to ensure a productive use of genetic data during the historical period where information about DNA sequence differences is available but efficacious treatments for the diseases they cause are not. Mike Knapp From owner-diagnostics@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!ubaclu.unibas.ch!bioftp.unibas.ch!citi2.fr!univ-lyon1.fr!news Newsgroups: bionet.diagnostics Subject: MINOXIDIL Message-ID: <45qkms$8hi@cismsun.univ-lyon1.fr> From: Erwan.LeRoy@cipcinsa.insa-lyon.fr (Erwan Le Roy) Date: 15 Oct 1995 09:36:28 GMT Organization: INSA NNTP-Posting-Host: mac110-106.insa-lyon.fr Mime-Version: 1.0 X-Newsreader: WinVN 0.99.2 Lines: 3 I am loosing my hair. COuld somebody help me. Is minoxidil good what is it? From owner-diagnostics@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!ncl.ac.uk!Larry.Winger From: Larry.Winger@ncl.ac.uk (Larry Winger) Newsgroups: bionet.diagnostics Subject: Re: Protozoa Date: 15 Oct 1995 11:10:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi Vigfrid, haven't seen any collection answer publicly yet, so I'll add the following 2p below: >collections for protozoa???? >And if so how do I get in touch with them -address, fax etc. There are strange sources of information, but few stranger than this one -- I'm doing secondary teacher training in a remote school up in the North Pennines, and tucked away in a back shelf in the prep room, I came upon this booklet: "Culturing Algae: a guide for schools and colleges" which was prepared by the Culture collection of Algae and Protozoa, of the Natural Environment Research Council, of UK. I think this council and the Science and Engineering Research Council were all subsumed under the British Biotechnology and Science Research Council about 5 years ago, but the culture collection might still be extant. The address (to which they moved in 1986) is: Culture Collection of Algae and Protozoa Freshwater Biological Association The Ferry House Ambleside, Cumbria United Kingdom The following number was published in the booklet -- I think it's defunct. Windermere (019662) 2468 The number of the Freshwater Biological Association at Ambleside is: (01539)442468 Confirmed this afternoon with directory enquiries; maybe they can re-direct. Hope this helps. Cheers Larry Larry 'wearing three hats in these cold times' Winger Larry.Winger@ncl.ac.uk http://georgia.ncl.ac.uk LarryWinger@mkn.co.uk http://mkn.co.uk/help/extra/craftnet/craft Larry A. Winger (First Class) Open University PGCE SecondaryScience From owner-diagnostics@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!MBDC2 From: MBDC2@SCRI.SARI.AC.UK ("David Cooke") Newsgroups: bionet.diagnostics Subject: Re: MINOXIDIL Date: 16 Oct 1995 01:48:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <4F8AAAC486D@law.scri.sari.ac.uk> Dear net users I fail to see the relevance of questions about minoxidil and hair loss to the diagnost net so please think before you post. Incidentally, I really would not worry about the balding you can't have brains and hair. Thank you David E. L. Cooke Mycology and Bacteriology Dept. Scottish Crop Research Institute Invergowrie, Dundee, DD1 5DA. Tel 0382 562731 FAX 0382 562426 From owner-diagnostics@net.bio.net Sun Oct 15 23:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: MINOXIDIL Date: 16 Oct 1995 03:44:36 -0700 Organization: Scottish Agricultural College Lines: 77 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510161044.DAA27776@net.bio.net> > Date: Mon, 16 Oct 95 08:54:11 > From: > Subject: MINOXIDIL > To: mx%"diagnost@net.bio.net" > Cc: > Return-Path: > Received: from festival.ed.ac.uk (festival) by SAC001.ed.sac.ac.uk (MX V4.1 > AXP) with SMTP; Sun, 15 Oct 1995 22:20:36 GMT > Received: from net.bio.net by festival.ed.ac.uk id aa04006; 15 Oct 95 22:18 BST > Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id DAA17695 for > diagnost-list; Sun, 15 Oct 1995 03:22:11 -0700 > Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id DAA17690 for > diagnost-arpanet; Sun, 15 Oct 1995 03:22:08 -0700 > To: diagnost@net.bio.net > Subject: MINOXIDIL > Message-ID: <45qkms$8hi@cismsun.univ-lyon1.fr> > From: Erwan Le Roy > Date: 15 Oct 1995 09:36:28 GMT > NNTP-Posting-Host: mac110-106.insa-lyon.fr > MIME-Version: 1.0 > X-Newsreader: WinVN 0.99.2 > > I am loosing my hair. > COuld somebody help me. Is minoxidil good what is it? > > > > Minoxidil is a product from the Upjohn Co.; it is licensed, at least in the UK (where it is called Regaine), for male pattern baldness, ie natural baldness. Trials have shown some limited success against alopecia areata, the patchy kind of hair loss, but not for the totalis/universalis kind where all hair drops out. It is expensive, particularly if you buy supplies from a private hair care clinic as opposed to a pharmacy; it is a long term treatment, months or years, and if successful, may need to be continued indefinitely or newly - grown hair may drop out again. There is no certain cure for alopecia; some people respond to certain treatments better than others, others do not respond at all, others get spontaneously better. Perhaps before rushing out to buy minoxidil you should try and get your hands on a drug called diphencyprone (DPC). It is made by the Kodak photographic company - it was never intended as a drug, but photographic workers who came into contact with it found that they sprouted hair in unwanted places. I suggest you try and get an appointment with the Dermatology clinic at your local hospital and ask if they can arrange a course of treatment with DPC. It is applied topically to the patchy areas. In the UK it is only available experimentally through clinics, as far as I am aware. DPC is possibly more effective than minoxidil, but this is anecdotal. If you cannot get hold of DPC, try a herbalist for some Primula obconica; this is supposed to work in the same way as DPC, by inducing a localised (maybe even systemic) allergic reaction to counteract the auto - immune response which causes alopecia. I wish you success with this distressing condition. I should add that I am not a qualified physician, but I have an interest in auto - immune disorders; needless to say any action you take on the above is at your own risk. Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: test of diagnost@daresbury.ac.uk Date: 17 Oct 1995 03:02:27 +0100 Lines: 1 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <45v2rj$fm7@mserv1.dl.ac.uk> Reply-To: biosci-help@net.bio.net Original-To: diagnost@dl.ac.uk test of diagnost@daresbury.ac.uk; please ignore. From owner-diagnostics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!biosci!not-for-mail From: biohelp@net.bio.net (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: test of bionet.diagnostics Date: 16 Oct 1995 19:01:12 -0700 Organization: BIOSCI International Newsgroups for Biology Lines: 4 Distribution: world Message-ID: <45v2p8$i9i@net.bio.net> NNTP-Posting-Host: net.bio.net test of bionet.diagnostics; please ignore. From owner-diagnostics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!NET.BIO.NET!biosci-help From: biosci-help@NET.BIO.NET (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: test of diagnost@net.bio.net Date: 16 Oct 1995 19:02:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: Reply-To: biosci-help@net.bio.net NNTP-Posting-Host: net.bio.net test of diagnost@net.bio.net; please ignore. From owner-diagnostics@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: MINOXIDIL Date: 17 Oct 1995 03:44:29 -0700 Organization: Scottish Agricultural College Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510171042.DAA11996@net.bio.net> NNTP-Posting-Host: net.bio.net On 16 Oct David Cooke wrote: > Date: Mon, 16 Oct 95 22:59:51 > From: > Subject: Re: MINOXIDIL > To: mx%"diagnost@net.bio.net" > Cc: > Return-Path: > Received: from festival.ed.ac.uk (festival) by SAC001.ed.sac.ac.uk (MX V4.1 > AXP) with SMTP; Mon, 16 Oct 1995 22:59:49 GMT > Received: from net.bio.net by festival.ed.ac.uk id aa18960; 16 Oct 95 22:57 BST > Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id NAA23687 for > diagnost-list; Mon, 16 Oct 1995 13:38:51 -0700 > Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id NAA23663 for > diagnost-arpanet; Mon, 16 Oct 1995 13:38:47 -0700 > To: diagnost@net.bio.net > From: "David Cooke" > Subject: Re: MINOXIDIL > Date: 16 Oct 1995 01:48:32 -0700 > Sender: daemon@net.bio.net > Message-ID: <4F8AAAC486D@law.scri.sari.ac.uk> > > Dear net users > > I fail to see the relevance of questions about minoxidil and hair > loss to the diagnost net so please think before you post. > Incidentally, I really would not worry about the balding you can't have > brains and hair. > > Thank you > David E. L. Cooke > Mycology and Bacteriology Dept. > Scottish Crop Research Institute > Invergowrie, Dundee, DD1 5DA. > Tel 0382 562731 > FAX 0382 562426 > > > I'm happy to interpret diagnostics in its widest sense so this question was fine by me. And when Einstein died, he had shoulder length hair. Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!mail2news.demon.co.uk!relay-2.mail.demon.net From: "C:COMMSWINSOCKKA9QSPOOLMAIL" Newsgroups: bionet.diagnostics Subject: UROLITHIASIS................Please help Date: Wed, 18 Oct 1995 07:47:12 GMT Organization: None Lines: 11 Message-ID: <617520582wnr@spectron.demon.co.uk> Reply-To: BRYONY@spectron.demon.co.uk X-NNTP-Posting-Host: relay-2.mail.demon.net X-Broken-Date: Wednesday, Oct 18, 1995 07.47.12 X-Newsreader: Newswin Alpha 0.7 Can anyone point me towards the causes and biochemical investigation of urolithiasis, perhaps with reference to some recent papers on the subject? Any information would be great.........many thanks in advance.-- Bryony Heasman bryony@spectron.demon.co.uk From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!plug.news.pipex.net!pipex!dish.news.pipex.net!pipex!lade.news.pipex.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Re: Protozoa Organization: Scottish Agricultural Science Agency Date: Tue, 10 Oct 1995 13:43:04 GMT Message-ID: X-Newsreader: WinVN 0.91.6 References: <199510061020.DAA01770@net.bio.net> Sender: news@sasa.gov.uk (Usenet) Lines: 41 In article <199510061020.DAA01770@net.bio.net>, ESA009@ED.SAC.AC.UK ("Dr Rob Harling") says: > >> Date: Thu, 05 Oct 95 20:35:23 >> From: >> Subject: Protozoa >> To: mx%"diagnost@net.bio.net" >> Hi >> >> For bacteria one can order strains from ATCC and NCTC etc. >> My question is does there excist similar collections for protozoa???? >> And if so how do I get in touch with them -address, fax etc. >> >> Vigfrid N=E6ss >> >> >> >> > >I have no USA address, but for Europe you could try the Information >Centre for European Culture Collections, Braunschweig, Germany, >tel 0531 61 8715/22 > >Rob Harling Or, if you have access to the WWW, check out the Culture Collection of Algae and Protozoa page. The URL is: http://wiua.nwi.ac.uk/ccap/ccaphome.html There is also a large collection of links to protozoa resources at: http://megasun.bch.umontreal.ca/protists/otherprodbs.html Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!PIPSC.Ca!rglw From: rglw@PIPSC.Ca (Roger Wheatcroft) Newsgroups: bionet.diagnostics Subject: Hair loss @~ ! (fwd) Date: 18 Oct 1995 06:02:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >> brains and hair. >> >> Thank you >> David E. L. Cooke >> Mycology and Bacteriology Dept. >> Scottish Crop Research Institute >> >I'm happy to interpret diagnostics in its widest sense so this >question was fine by me. And when Einstein died, he had shoulder >length hair. > > >Dr Rob Harling >SAC (Scottish Agricultural College)/ > Just imagine what Einstein might have achieved if only he'd been bald like Newton! Roger W. Ag.Canada From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test ignore Date: 18 Oct 1995 05:13:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!MBDC2 From: MBDC2@SCRI.SARI.AC.UK ("David Cooke") Newsgroups: bionet.diagnostics Subject: discussion points Date: 18 Oct 1995 07:10:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 78 Sender: daemon@net.bio.net Distribution: world Message-ID: <52E09881194@law.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net Dear diagnost net While I was maybe a little flippant about the request for information about about Minoxidil and I do recognise that this type of discussion group is supposed to be broad I do think it is worth taking a look at the Charter (I think this is the current version Kevin?). There has been little discussion of any diagnostic import recently so maybe I could repeat a plea I put out about a year ago. Has anyone seen a piece of software for selecting PCR primers other than all the current ones select a pair of primers for a single length of sequence data. What I need is someting to analyse ribosomal sequences from several species of pathogenic fungi and select the best primer for specific amplification of species X and no other species. I am sure there is a great market for such a piece of software (if there are any budding programmers out there). Also is there any more news about the EU patent challenge from promega regarding Taq polymerase? The charter follows. <<< PROPOSAL FOR DIAGNOSTICS NEWSGROUP The Diagnostics newsgroup is a forum for discussion of problems and techniques (primarily serological or nucleic acid-based) involved in all fields of diagnostics. Subjects for discussion and comment include methodology, automation, quality assurance and other subjects of interest and concern to those developing and/or using diagnostic tests JUSTIFICATION Despite the large number of scientists involved in diagnostics, there is no newsgroup devoted to this subject. The prototype Diagnostics newsgroup was set up to fill this gap and has gained enough support to justify a vote for full newsgroup status. The prototype newsgroup has included postings on the detection and identification of diseases, pathogens (animal and plant) and also diagnostic functions which do not fit into either category. This composition has helped to establish common ground (and therefore intellectual and methodological cross-fertilization) between people and laboratories. For example, The problems facing scientists developing PCR assays for Listeria are similar to those facing scientists developing PCR assays for bacterial soft rots in plants. Within the various disciplines there are common problems regarding methods, immunogens, cross-reactivity, automation, materials, quality assurance and other issues that are not recognized by those using similar techniques for other purposes. The Diagnostics newsgroup will address and help solve these problems by providing a forum for their discussion between individuals of various experience and disciplines. Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk >>>> Regards David Cooke David E. L. Cooke Mycology and Bacteriology Dept. Scottish Crop Research Institute Invergowrie, Dundee, DD1 5DA. Tel 0382 562731 FAX 0382 562426 From owner-diagnostics@net.bio.net Tue Oct 17 23:00:00 1995 Path: biosci!biosci!not-for-mail From: Rainer Schubert Newsgroups: bionet.announce,bionet.software,bionet.neuroscience,bionet.biology.cardiovascular,bionet.biology.computational,bionet.diagnostics,bionet.general Subject: VBC'96 Call for Papers Date: 17 Oct 1995 18:36:46 -0700 Organization: University Hospital Eppendorf / Hamburg Lines: 227 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.announce:2555 bionet.software:13637 bionet.neuroscience:10780 bionet.biology.cardiovascular:575 bionet.biology.computational:789 bionet.diagnostics:338 bionet.general:17975 -------------------------------------------------------------------- Fourth International Conference on VISUALIZATION IN BIOMEDICAL COMPUTING Sept. 22 - Sept. 25, 1996 Hamburg, Germany -------------------------------------------------------------------- CALL FOR PAPERS Scope Following Georgia Tech, the University of North Carolina, and the Mayo Institutions, the University of Hamburg will host the 4th conference on Visualization in Biomedical Computing (VBC'96). Its goal is to enhance and promote the science of computer based visualization of biomedical information, especially about the various aspects of the human body. An interdisciplinary, international group of scientists, engineers, and clinicians will present and discuss the state of the art concerning algorithms, the rapidly increasing number of applications, and the practical problems of system design. ------------------------------------------------------------------------ Topics Theory and Algorithms Applications Systems * Models of * Diagnostics with * Hardware/Software Visualization MRI, CT, PET/SPECT, Tools * Rendering Algorithms MEG Ultrasound etc. * Interactive * Image Analysis * Microscopy Imaging Workstations and * Multi-Modality Image * Surgical and Networks Registration and Stereotactic * Virtual Reality User Fusion Planning Interfaces * Shape Description * Surgery Simulation * Novel Image and Characterization * Image Guided Acquisition Equipment * Spatial Knowledge Surgery * Special Processors Modeling * Endoscopy * Artificial Simulation Intelligence in * Radiation Visualization Treatment Planning * Visual Perception * Biomechanics and and Communication Orthopedics * Virtual / Augmented * Dentistry and Reality Paradigms Orthodontics * Prothetics Development * Modeling the human body (with emphasis on the brain) * Anatomy Atlases (with emphasis on VISIBLE HUMAN) * Medical and Biological Education ------------------------------------------------------------------------ Format According to the interdisciplinary nature of the conference, it has a single track of sessions with 25 minute papers. Posters and demonstrations will be located next to the auditorium. Tutorials will be held the day before the official opening (Sept. 22) as in the previous meetings. ------------------------------------------------------------------------ Submission Papers Authors are invited to submit five copies of an extended abstract not shorter than two pages which may include figures. Submission of full papers is encouraged. The submission must begin with a 50 word statement about the major results of the work. Posters Authors are invited to submit posters under the conditions applying for papers. They will be included in the proceedings as short papers. Demonstrations There will be the possibility of hands-on demonstrations. Please submit an outline (not exceeding 2000 words), including the hardware requirements. Tutorials Proposals for half-day (four hour) tutorials are invited to any aspect of visualization in biomedical computing as described in the topics section. Proposals should include an outline accompanied by a curriculum vitae. VBC`94 offered: 3D Imaging in Biomedical Computing, Volume Visualization, Visualization in the Neurosurgical OR, Modern 3D Microscopy and Visualization, Multiscale Geometric Image Analysis ------------------------------------------------------------------------ ! DEADLINES ! ! ! ! Paper/Poster Submission : February 1, 1996 ! ! ! ! Tutorial Submission : February 1, 1996 ! ! ! ! Notification of Acceptance : April 1, 1996 ! ! ! ! Full papers : June 15, 1996 ! ! ! ! Demonstration Proposals : June 1, 1996 ! ------------------------------------------------------------------------ Proceedings Proceedings will be published in Lecture Notes in Computer Science, Springer Verlag Heidelberg-New York. They will be available at the time of the conference. ------------------------------------------------------------------------ Visits Visits to laboratories in Hamburg will be arranged for the day after the conference (IMDM, Philips Research, Dept. of Computer Science) ------------------------------------------------------------------------ Location University of Hamburg, main building, in walking distance to the city which surrounds the beautiful Alster lake. ------------------------------------------------------------------------ Previous Conferences 1990 Georgia Institute of Technology, Atlanta, GA Proceedings: First Conference on Visualization in Biomedical Computing, IEEE Computer Society Press, 1990 1992 University of North Carolina, Chapel Hill, NC Proceedings: Visualization in Biomedical Computing II, Proc. SPIE 1808, 1992 1994 Mayo Foundation, Rochester, MN Proceedings: Visualization in Biomedical Computing 1994, Proc. SPIE 2359, 1994 ------------------------------------------------------------------------ Conference Committee Chairman Karl Heinz Hoehne, University of Hamburg Program Committee Ron Kikinis (Chair), Harvard Medical School Nicholas Ayache, INRIA, Sophia-Antipolis Jean Louis Coatrieux, University of Rennes Takeyoshi Dohi, University of Tokyo James Duncan, Yale University Alan C. Evans, Montreal Neurological Inst. Norberto Ezquerra, Georgia Tech, Atlanta Henry Fuchs, UNC, Chapel Hill Guido Gerig, ETH Zuerich Eric Grimson, MIT, Cambridge David Hawkes, Guy's Hospital London Arie Kaufman, State University of New York Andres Kriete, University of Giessen Hal Kundel, University of Pennsylvania Heinz Lemke, Free University of Berlin David Levin, University of Chicago Heinrich Mueller, University of Dortmund Chuck Pelizzari, University of Chicago Stephen Pizer, UNC, Chapel Hill Richard Robb, Mayo Foundation / Clinic Siegfried Stiehl, University of Hamburg Paul Suetens, University of Leuven Andrew Todd-Pokropek, University College London Jun-Ichiro Toriwaki, Nagoya University Michael Vannier, Washington University, St Louis Max Viergever, Utrecht University Coordinators Bernhard Pflesser, Thomas Schiemann, Rainer Schubert, IMDM ------------------------------------------------------------------------ Supporting Organisations University of Hamburg The Institute of Electrical and Electronics Engineers (IEEE) German Society of Computer Science (GI) ------------------------------------------------------------------------ Submissions and Inquiries to Institute of Mathematics and Computer Science in Medicine (IMDM) Department of Computer Science in Medicine Pav. 70 University Hospital Eppendorf Martinistrasse 52 D-20246 Hamburg Germany Phone : +49-40-4717 3652 Fax : +49-40-4717 4882 E-mail: vbc96@uke.uni-hamburg.de For latest news consult the following www page: ======================================== http://www.uni-hamburg.de/~medizin/vbc96 ======================================== --------------------------------------------------------------------------- From owner-diagnostics@net.bio.net Wed Oct 18 23:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dish.news.pipex.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Newsreader Problems Organization: Scottish Agricultural Science Agency Date: Thu, 19 Oct 1995 14:56:48 GMT Message-ID: X-Newsreader: WinVN 0.91.6 Sender: news@sasa.gov.uk (Usenet) Lines: 14 We have had problems with our newsfeed here at SASA, which have meant that articles posted by me or robert Burns to this group have appeared on our newsreader but haven't been propagated outside SASA! at the same time, posts submitted my e-mail have not appeared on our newsreader. Apologies for the problems this has caused - it has meant that we haven't been able to do our discussion leader jobs effectively. Hopefully, the problems have now been sorted out and normal service will be resumed. Kevin O'Donnell Scottish Agricultural Science Agency Edinburgh Scotland From owner-diagnostics@net.bio.net Wed Oct 18 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test please ignore Date: 19 Oct 1995 10:18:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net mailit Robert Burns Monoclonal Antibody Unit Scottish Agricultural Science Agency East Craigs Edinburgh Scotland burns@sasa.gov.uk From owner-diagnostics@net.bio.net Wed Oct 18 23:00:00 1995 Path: biosci!sasa.gov.uk!burns From: burns@sasa.gov.uk (Robert Burns) Newsgroups: bionet.diagnostics Subject: test please ignore Date: 19 Oct 1995 10:18:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510191419.aa00186@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net netscape From owner-diagnostics@net.bio.net Thu Oct 19 23:00:00 1995 Path: biosci!COMPUSERVE.COM!100023.3435 From: 100023.3435@COMPUSERVE.COM ("J. H. MACKENZIE") Newsgroups: bionet.diagnostics Subject: BIONET MEMBERSHIP Date: 20 Oct 1995 02:42:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <951020093846_100023.3435_JHB38-2@CompuServe.COM> NNTP-Posting-Host: net.bio.net PLEASE ADD MY DETAILS TO BIONET NEWS GROUP From owner-diagnostics@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!CIPCINSA.INSA-LYON.FR!Erwan.LeRoy From: Erwan.LeRoy@CIPCINSA.INSA-LYON.FR ("Erwan Le Roy") Newsgroups: bionet.diagnostics Subject: Re: MINOXIDIL Date: 23 Oct 1995 05:02:42 -0700 Organization: INSA Centre Informatique 1er Cycle Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <244DD1F4306@cipcinsa.insa-lyon.fr> Can you give me some precision about primula obconica and dpc. I am 19 and i am loosing my hair. Minoxidil does nothing on me. Can the treatment you tell me help me? Do you know the new treatment VIVISCAL which comes from finland? It's a oral medicament done whith fiches! Please answer me! Thanks. From owner-diagnostics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!sgigate.sgi.com!sgiblab!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal Subject: ICSI and genetic abnormalities Date: 25 Oct 1995 03:26:48 GMT Organization: UCSF, ITS Lines: 57 Distribution: world Message-ID: <46kapo$lmm@itssrv1.ucsf.edu> NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.diagnostics:349 bionet.diagnostics.prenatal:53 [ Article crossposted from bionet.general ] [ Author was Dr Jim Cummins ] [ Posted on 22 Oct 1995 21:14:42 -0700 ] At the recent Andrology in the Nineties conference in Belium there was much debate about the use of intracytoplasmic sperm injection (ICSI) from oligoasthenozoospermic (OAT) men and genetic risk. Peter Vogt reported on the increased incidence of Y-chromosome delections and sex chromosome anomalies in such men, and there seems to be evidence of a general increase of paternally inherited anomalies in offspring (see also the somewhat restricted series for women of advanced maternal age reported by In't Veld in Lancet 346: 773, 1995). While the excellent data from the Brussels group reported by Van Steirteghem does not give rise to urgent concern, Moosani et al ( Fertility & Sterility 1995 64(4):811-817) in an analysis of sperm nuclei by fluorescence in situ hybridization report a significant increase in the frequency of disomy for chromosome I and XY disomy for infertile men. The take-home message is that any AOT men and their partners seeking ICSI for infertility should be given rigorous genetic counselling and advice about the possible consequences, especially for male offspring. Screening for satellite deletions in the AZF region of the Y chromosome should be mandatory IMHO. In Belgium now 1% of all births arise from some form of assisted reproductive technology, and this is likely to rise, according to Frank Comhaire. If we assume that the incidence of infertility is 10-15% and that now nearly all cases can be "treated" by ICSI it is logical to assume that a similar proportion of all births will result. Even if only 10% of OAT men carry a risk of genetic abormality this is still a highly disturbing scenario for the next generation. These conditions can *only* be transmitted iatrogenically - unlike diabetes, for example, where couples have the choice of reproducing or not reproducing. With the incredible acceleration of ICSI into the "run of the mill" IVF clinics without access to intensive genetic and paediatric services the long-term implications for health care costs makes the low-birthweight problems of conventional IVF babies look almost trivial. Alarm bells are ringing, I'm afraid. Jim "Spermatology rules o~ o~ o~ o~" Cummins Associate Professor in Veterinary Anatomy Murdoch University, Western Australia 6150 Tel +61-9-360 2668, Fax +61-9-310 4144 E mail URL The Penultimate Law "All general statements are false." From owner-diagnostics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!news From: Erwan.LeRoy@cipcinsa.insa-lyon.fr (Erwan Le Roy) Newsgroups: bionet.diagnostics Subject: ALOPECY VIVISCAL Date: 25 Oct 1995 11:55:26 GMT Organization: INSA Centre Informatique du 1er Cycle. Lines: 5 Message-ID: <46l8je$jko@cismsun.univ-lyon1.fr> NNTP-Posting-Host: pc110-121.insa-lyon.fr Mime-Version: 1.0 X-Newsreader: WinVN 0.99.2 Does anybody know this new treatment for alopecy made with fishes. It comes from finland. Tell me what you know about it and where can i find it. From owner-diagnostics@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!math.ohio-state.edu!cs.utexas.edu!news.ti.com!usenet From: mattwoolsey@ti.com (Matthew Woolsey) Newsgroups: bionet.diagnostics,bionet.neuroscience,sci.bio.technology,sci.engr.biomed Subject: EEG Equipment Sources? Date: Wed, 25 Oct 1995 21:23:18 GMT Organization: Texas Instruments Lines: 11 Distribution: inet Message-ID: <46m9rq$ppg@tilde.csc.ti.com> NNTP-Posting-Host: holodeck1.csc.ti.com X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.diagnostics:351 bionet.neuroscience:10901 sci.bio.technology:4055 sci.engr.biomed:4646 I am looking for sources for EEG sensors and amplifier/filters. Need company names, phone numbers, and any recommendations or preferences. Since this is experimental, I need relatively cheap amplifiers/filters that can still be hooked up to a live subject. Thanks in advance, Matthew Woolsey mattwoolsey@ti.com From owner-diagnostics@net.bio.net Wed Oct 25 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.uiowa.edu!black.weeg.uiowa.edu!pseaba From: "P. Seaba" Newsgroups: bionet.diagnostics,bionet.neuroscience,sci.bio.technology,sci.engr.biomed Subject: Re: EEG Equipment Sources? Date: Thu, 26 Oct 1995 13:39:03 -0500 Organization: University of Iowa, Iowa City, IA, USA Lines: 37 Distribution: world Message-ID: References: <46m9rq$ppg@tilde.csc.ti.com> NNTP-Posting-Host: black.weeg.uiowa.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: pseaba@black.weeg.uiowa.edu In-Reply-To: <46m9rq$ppg@tilde.csc.ti.com> Xref: biosci bionet.diagnostics:352 bionet.neuroscience:10913 sci.bio.technology:4059 sci.engr.biomed:4659 By "live", I take it you mean human? Go to the Medical Device Register and find out who's registered with the FDA. Find a device that looks too good to be true!? Ask them if they've filed an intent to market, 510(k). Better yet, call the FDA (check gov't. section of your local telephone book) and ask if the company has a 510(k) number for the device. Then ask if it's been tested to UL544; the 2500 vac isolation barrier and 4000 vac withstand tests usually weed out the tinkerers. In spite of what the salesperson tells you, battery operated or optically isolated devices are not inherently safe. They still have to pass the same tests for human use. Make sure you inform your legal counsel in advance if you choose to utilize "bargain" devices. Pete Seaba U. of Iowa On Wed, 25 Oct 1995, Matthew Woolsey wrote: > I am looking for sources for EEG sensors and amplifier/filters. > Need company names, phone numbers, and any > recommendations or preferences. Since this is experimental, > I need relatively cheap amplifiers/filters that can still be hooked > up to a live subject. > > Thanks in advance, > Matthew Woolsey > mattwoolsey@ti.com > > > > From owner-diagnostics@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!ARNIE.CFI.UNSW.EDU.AU!p.french From: p.french@ARNIE.CFI.UNSW.EDU.AU (Peter French) Newsgroups: bionet.diagnostics Subject: (none) Date: 26 Oct 1995 18:47:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510270053.LAA02777@arnie.cfi.unsw.EDU.AU> NNTP-Posting-Host: net.bio.net unsubscribe - I've had enough bald men and their problems Peter French, Centre for Immunology, St Vincent's Hospital, Sydney. President, ANZSCBI phone 61-2-361-2388 fax 61-2-361-2391 mobile 018-412-961 From owner-diagnostics@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!ARNIE.CFI.UNSW.EDU.AU!p.french From: p.french@ARNIE.CFI.UNSW.EDU.AU (Peter French) Newsgroups: bionet.diagnostics Subject: Re: ALOPECY VIVISCAL Date: 26 Oct 1995 18:48:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510270051.LAA02696@arnie.cfi.unsw.EDU.AU> NNTP-Posting-Host: net.bio.net Erwan Le Roy wrote: >Does anybody know this new treatment for alopecy made >with fishes. It comes from finland. >Tell me what you know about it and where can i find >it. Yes - you rub a trout on your head every day. Peter French, Centre for Immunology, St Vincent's Hospital, Sydney. President, ANZSCBI phone 61-2-361-2388 fax 61-2-361-2391 mobile 018-412-961 From owner-diagnostics@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!tyunnos.demon.co.uk From: rwj@tyunnos.demon.co.uk (rwj) Newsgroups: bionet.diagnostics Subject: Re: MINOXIDIL Date: Sat, 28 Oct 1995 21:55:17 GMT Lines: 13 Distribution: world Message-ID: <814917317.16781@tyunnos.demon.co.uk> References: <199510161044.DAA27776@net.bio.net> NNTP-Posting-Host: tyunnos.demon.co.uk X-NNTP-Posting-Host: tyunnos.demon.co.uk X-Newsreader: WinVN 0.99.6 In article <199510161044.DAA27776@net.bio.net>, ESA009@ED.SAC.AC.UKA says... [..] > >There is no certain cure for alopecia; some people respond to >certain treatments better than others, others do not respond at all, >others get spontaneously better. Perhaps before rushing out to buy >minoxidil you should try and get your hands on a drug called [..] There is discussion of all of this on alt.baldspot. Over and out. From owner-diagnostics@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Alopecia, slap-heads and diagnostics Date: 30 Oct 1995 03:09:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I have every sympathy with people affected by alopecia , a distressing = medical condition in which all body hair is lost. However, I don't believ= e that bionet.diagnostics is the fruitful arena in which this subject = could be raised. More, and perhaps medically qualified, advice may be = found in the sci.med newsgroups. Ordinary male-pattern baldness, while an issue for some of us, is a more = appropriate topic for bionet.general or sci.bio.misc (though may I just = recommend to fellow slap-heads that blue, menthol-containing shampoo from= the Body Shop:-)). What would be appropriate here is more on-charter posts. More and more = people are subscribing to bionet.diagnostics all the time and it is avail= able as a usenet group, yet there has been no corresponding increase in = posts. May I make a suggestion to go some way towards stimulating discussion? = If you are publishing a diagnostics-related paper, why not post the abstr= act here, for others to read and comment on? Other suggestions for improving the level of discussion in the group woul= d be most welcome. Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: bss3pk@surrey.ac.uk (Peter Kwasowski) Newsgroups: bionet.diagnostics Subject: Test: please ignore Date: 31 Oct 1995 17:15:42 -0000 Lines: 4 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <475lju$4j1@mserv1.dl.ac.uk> Original-To: diagnost@dl.ac.uk ... but working-up to an actual post real soon. From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!COMPUSERVE.COM!100416.735 From: 100416.735@COMPUSERVE.COM (The Diagnostics Club) Newsgroups: bionet.diagnostics Subject: Kevin O'Donnells quest Date: 1 Nov 1995 01:06:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <951101085737_100416.735_BHG66-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net I agree with Kevin's comments about the lack of postings. From our own viewpoint there is disappointment in the few notices from those organizations at the sharp end. We know of several 'sharp end' organisations that monitor this newsgroup (they are also members of The Diagnostics Club), but there must be many more out there that have something to publically offer, or something they need. Wearing our own hat we monitor the newsgroup so as to advise our members of opportunities for collaboration, and to advise the newsgroup of happenings of mutual interest to the newsgroup. To the benefit of the total diagnostics community there must be more interaction between researchers and those that apply the theories to the needs and practicalities of the commercial world. This newsgroup would seem to be an ideal vehicle for that action, and The Diagnostics Club is here to aid the transfer and collaboration. Regards Tony Owen Diagnostics Club Administrator tel: +44 1908 647417, fax: +44 1908 271612 From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Commerical posts etc. Date: 1 Nov 1995 08:12:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 59 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Steve Firth wrote: > > Some of my clients in the diagnostics > industry only have mail access, which could help to explaing why the > newsgroup is so quiet. > Remember that all of the bionet newsgroups are also available as e-mail = mailing lists. I agree though, that there's something clubby (for want = of a better word) about a mailing list that seems to be lost with a newsgroup. > I also believe that it is the restriction placed on commercial posts = that > keeps bionet.diagnostics so quiet. I don't wish to see advertising, = but I > would like to exchange general commercial information (looking for > materials, requests for researchers to participate in assay development > projects etc.) which I understand from a conversation with the > bionet.general moderator is banned from all bionet groups. > Hmm. Now this is an interesting point. It remains to be seen if the stric= t ban on commercial activities will continue once NSF funding for bionet = stops (quite soon ISTR). In any event, I think we'd all agree that we = don't want to be innundated with adverts for Dodgy Derek's Dialysis tubin= g or whatever, but the other things described above look less undesirable= to me. Requests for people to take part in assay development have certainly appe= ared here before, and as it can't be described as advertising I haven't = seen it as a problem. Exchanging commercial information? - it depends = what you mean. I think that posts along the lines of 'What does anyone = think of product X' are quite acceptable - as long as the post doesn't = come from the makers of product X - though themakers of product X are = welcome to respond to direct queries about their product. Likewise (and this is where things do get a bit silly), it is acceptable = for someone to post saying 'what type of dialysis tubing do people use?' = and for someone else to reply saying 'Hey, Dodgy Derek's dialysis tubing = is great - buy it' but not for the aforementioned Dodgy Derek himself = to respond saying 'Hey I make dialysis tubing - buy it, it's great'. I hope that this is now clear:-). My own view is that the sort of things Steve mentions are acceptable, = within the restrictions mentioned above. Further information is available= in the bionet/biosci FAQ (posted in bionet.announce or at http://www.bio= .net). Apologies to any Dereks who may read this list:-) Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!SIAL.COM!douglasm From: douglasm@SIAL.COM (Michael Douglas) Newsgroups: bionet.diagnostics Subject: Re: (none) Date: 1 Nov 1995 08:06:57 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <9511010826.AA00817@mail.cip.org.ec> NNTP-Posting-Host: net.bio.net It is crude but you need to know the number and average physical size of the chromosomes in question. The can be related to the average physical size of a given yeast chromosome which are all very well defined according to size and DNA content. ___________________________________________________________________________ Michael Douglas douglasm@sial.com Sigma Diagnostics -- 545 South Ewing -- St. Louis, MO 63103 USA FAX: 314-531-2586 or 800-816-1603 Tele: 800-521-8956 or 314-771-5765 x4435 On 1 Nov 1995, Maria Eugenia Ordonez wrote: > > How can I estimate the size of the genome of a plant having the > kariotype? > > > -- > Maria Eugenia Ordonez > Centro Internacional de la Papa > Casilla 17-21-1977-CEQ > Quito, Ecuador > Telf. +593-2-690362, Internet: ORDONEZ@cip.org.ec > > From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!zetnet.co.uk!dispatch.news.demon.net!demon!user From: filter@firthcom.demon.co.uk (Steve Firth) Newsgroups: bionet.diagnostics Subject: Re: Kevin O'Donnells quest Date: Wed, 01 Nov 1995 15:14:03 +0000 Organization: Firth Consulting Lines: 31 Message-ID: References: <951101085737_100416.735_BHG66-1@CompuServe.COM> NNTP-Posting-Host: firthcom.demon.co.uk X-NNTP-Posting-Host: firthcom.demon.co.uk In article <951101085737_100416.735_BHG66-1@CompuServe.COM>, 100416.735@COMPUSERVE.COM (The Diagnostics Club) wrote: >I agree with Kevin's comments about the lack of postings. Me too. I found that the diagnostics mail list was more informative and had a higher volume of interest than bionet.diagnostics, perhaps the more intimate nature of mail and the fact that it arrives rather than having to be searched for makes the difference. Some of my clients in the diagnostics industry only have mail access, which could help to explaing why the newsgroup is so quiet. I also believe that it is the restriction placed on commercial posts that keeps bionet.diagnostics so quiet. I don't wish to see advertising, but I would like to exchange general commercial information (looking for materials, requests for researchers to participate in assay development projects etc.) which I understand from a conversation with the bionet.general moderator is banned from all bionet groups. Perhaps there would be more interest in a diagnostics newsgroup within the biz hierarchy? -- Stephen Firth, Firth Consulting Support Services for Biotechnology and Diagnostics tel: +44 1962 771 313 From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: 1 Nov 1995 07:12:11 -0800 Organization: University of Arkansas Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <48A2F90C78@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > We're using RAPD-PCR to study diversity in epiphytic Pseudomonas > species. We prepare the template DNA using Flowgen's Puregene kit, > which is basically an SDS cell lysis, ammonium acetate protein > precipitation, RNAse, isopropanol DNA precipitation, then dissolve in > water. The resulting DNA is of good spectrophotometric quality. > > PCR works if we use freshly - prepared DNA, ie extracted from the > bacteria then run on the same day. If we store the DNA at 4C for > only one week, then PCR fails, we get zilch in the lanes at any > concentration of template. The DNA still looks the same after one week > when run on a gel, ie there doesn't seem to be nuclease breakdown > of the DNA. Does anyone know what's happening to the DNA in storage? > > Thanks > Rob (still got my hair) Harling > > Dr Rob Harling > SAC (Scottish Agricultural College)/ > University of Edinburgh > West Mains Road > Edinburgh EH9 3JG > Scotland, UK > tel: +44 (0)131 535 4000 > fax: +44 (0)131 667 2601 > e mail: esa009@ed.sac.ac.uk This phenomenon has been observed repeatedly in a number of groups (consult RAPD@net.bio.net archives). If I had to make predictions I would guess your DNA was fairly dilute (1-25 ng/ul). Most people find DNA to be much more stable at higher concentrations and that dilute samples for RAPD should be stored frozen. We have observed the same phenomenon with first strand cDNA preps for RT-PCR or ddRT-PCR. But for these it is even worse. They don't last more than a week even in the -20. We have tried adding a little DTT to inhibit oxidation and that seems to help a little. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!CIP.ORG.EC!ordonez From: ordonez@CIP.ORG.EC (Maria Eugenia Ordonez) Newsgroups: bionet.diagnostics Subject: (none) Date: 1 Nov 1995 07:04:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511010826.AA00817@mail.cip.org.ec> NNTP-Posting-Host: net.bio.net How can I estimate the size of the genome of a plant having the kariotype? -- Maria Eugenia Ordonez Centro Internacional de la Papa Casilla 17-21-1977-CEQ Quito, Ecuador Telf. +593-2-690362, Internet: ORDONEZ@cip.org.ec From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: 1 Nov 1995 06:29:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <199511011139.DAA18368@net.bio.net> NNTP-Posting-Host: net.bio.net Rob Harling wrote: > PCR works if we use freshly - prepared DNA, ie extracted from the > bacteria then run on the same day. If we store the DNA at 4C for > only one week, then PCR fails, we get zilch in the lanes at any > concentration of template. The DNA still looks the same after one week > when run on a gel, ie there doesn't seem to be nuclease breakdown > of the DNA. Does anyone know what's happening to the DNA in storage? > Rob, Do you know if it still cuts with restriction endonucleases? Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!myco.com!slutsky From: slutsky@myco.com (Slutsky Alex) Newsgroups: bionet.diagnostics Subject: SUBSCRIBE Date: 1 Nov 1995 05:51:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <3097A533@myco.com> NNTP-Posting-Host: net.bio.net SUBSCRIBE TO THE LIST From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: now you see it now you don't Date: 1 Nov 1995 03:40:19 -0800 Organization: Scottish Agricultural College Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511011139.DAA18368@net.bio.net> NNTP-Posting-Host: net.bio.net We're using RAPD-PCR to study diversity in epiphytic Pseudomonas species. We prepare the template DNA using Flowgen's Puregene kit, which is basically an SDS cell lysis, ammonium acetate protein precipitation, RNAse, isopropanol DNA precipitation, then dissolve in water. The resulting DNA is of good spectrophotometric quality. PCR works if we use freshly - prepared DNA, ie extracted from the bacteria then run on the same day. If we store the DNA at 4C for only one week, then PCR fails, we get zilch in the lanes at any concentration of template. The DNA still looks the same after one week when run on a gel, ie there doesn't seem to be nuclease breakdown of the DNA. Does anyone know what's happening to the DNA in storage? Thanks Rob (still got my hair) Harling Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!winternet.com!news From: "Applied Resources, Inc." Newsgroups: bionet.diagnostics Subject: Minneapolis, MN- Senior Scientist, assay design Date: 1 Nov 1995 18:33:11 GMT Organization: Applied Resources, Inc. Lines: 19 Message-ID: <478eh7$npj@blackice.winternet.com> NNTP-Posting-Host: ppp-66-66.dialup.winternet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) I am a technical recruiter in Minneapolis, MN. Our client is seeking to add a Senior Scientist (R&D) to it's staff. Must have experience developing multiple products from concept to manufacture. Key skills follow. Ph. D.- minimum 5 years industrial experience in immunoassay design and development. Five commercial products for which candidate has primary development responsibility. Minimum 2 years developing assays on automated instrumentation. If your background meets or exceeds these minimum requirements, please respond to: Steve Musch Applied Resources ari@winternet.com Minneapolis, MN USA From owner-diagnostics@net.bio.net Tue Oct 31 22:00:00 1995 Path: biosci!UAlberta.CA!Michele.Hales From: Michele.Hales@UAlberta.CA (Michele Hales) Newsgroups: bionet.diagnostics Subject: HSV with RT-PCR Date: 1 Nov 1995 12:11:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511012009.NAA06359@bock.ucs.ualberta.ca> NNTP-Posting-Host: net.bio.net I am posting a query for a friend of mine: When looking for HSV (A DNA virus) she has read published articles saying to run RT-PCR. Once you get the PCR product are there any other protocols/procedures that need to be done? I appreciate any information you may be able to pass on. Thanks in advance, Michele Hales V (- -) ( V ) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~m~"~~~~~~~~~~~~~~~~~~~~~~~~~~~ VVV Michele L. Hales "There are people who reshape the world Dept of Lab Med & Pathology by force or argument, but the cat just 5B1.47 WMC lies there, dozing, and the world quietly University of Alberta reshapes itself to suit his comfort and Edmonton, Alberta, Canada T6G 2R8 convenience." Telephone: (403) 492-8385 Unknown Fax: (403) 492-2253 E-mail: Michele.Hales@UAlberta.CA URL: http://synapse.uah.ualberta.ca/synapse/000p002i.htm My Other Creations: http://synapse.uah.ualberta.ca/synapse/000p0025.htm http://synapse.uah.ualberta.ca/synapse/000p0035.htm ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~