From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Microtitre plate PCR Date: 2 Nov 1995 03:12:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: We are looking at transferring some of our PCR assays to a microtitre = plate format. Does anyone have any experience of this that they can share= ? For example, is this method less robust than using microtubes? What sort = of throughput gains did you achieve? What sort of microtitre plate/therma= l cycler would you recommend - or not, as the case may be. All tips/hint= s about how to make a succesful tranfer would be appreciated. Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!aol.com!MHughes77 From: MHughes77@aol.com Newsgroups: bionet.diagnostics Subject: Serological Controls in Test Kits Date: 2 Nov 1995 11:07:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <951102140530_96176626@emout04.mail.aol.com> NNTP-Posting-Host: net.bio.net To fellow Diagnostics Newsgroup members: I am a scientist at a small diagnostics company involved in developing rapid, easy-to-use tests designed to be used primarily in doctors' clinics or veterinary practices for diagnosing a wide variety of infectious diseases, as well as for screening donated blood for infectious agents. Our kits come with ready-to-use reagents in dropper bottles and have good stability for 12-18 months at 4 degrees C and 3-6 months at room temperature. We would like to be able to offer human serological controls for some of the test kits, preferably with the same desirable stability characteristics. Perhaps some of you would have some information or suggestions regarding the following questions we have: 1. What is the best way to inactivate viruses without appreciably affecting the antibody and/or antigen activity? 2. How do we stabilize the controls so that we can provide them in the ready-to-use liquid format? 3. Can the controls be diluted samples? 4. Are there any universal regulations or guidelines regarding serological controls? We appreciate any help that is offered. : ) Mark Hughes Empyrean Diagnostics Inc. mhughes77@aol.com From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news3.digex.net!access1.digex.net!rschifre From: Richard Schifreen Newsgroups: bionet.cellbio,bionet.diagnostics,bionet.immunology Subject: Risk Factor Date: Thu, 2 Nov 1995 12:07:43 -0500 Organization: Express Access Online Communications, USA Lines: 11 Message-ID: NNTP-Posting-Host: access1.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.diagnostics:373 bionet.immunology:6076 A colleague is trying to follow-up on a report he heard that a lipid related risk marker has been documented that correlates closely with lifespan. Can anyone suggest a source or reference? The only thing that comes to mind is the relationship of apolipoproteins to artherosclerotic disease and now Alzheimers. Rich Schifreen phone: 301-840-4163 Life Technologies fax: 301-670-1493 E-Mail: rschifre@access.digex.net From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!qut.edu.au!r.epping From: r.epping@qut.edu.au (Ron Epping) Newsgroups: bionet.diagnostics Subject: A New centre for Diagnostic Technologies Date: 2 Nov 1995 03:15:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 110 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511011802.KAA18507@netcom.netcom.com> Forwarded from the biz-biotech list, I thought that this might be of inte= rest to this one. Anyone have any more information about this organisation? The newly established Australian National Centre for Diagnostic Technolog= ies (CDT) is currently recruiting highly-motivated scientists and students = to participate in research and development for the next generation of DNA-ba= sed human diagnostics for both inherited and acquired (cancer) genetic diseas= es and infectious diseases. Positions (Ref. No. and closing dates for 1995) = are available for: Senior Research Fellows (95525 November 21) Research Fellows (95526 November 21) Postdoctoral Fellows (95527 November 21) Senior Research Assistants (95528 November 6) Research Assistants (95528 November 6) PhD Students ( October 31) Honours Students (31 October) The CDT is a new Commonwealth funded Cooperative Research Centre bringing together the diagnostic and molecular biological expertise and innovation= of the Queensland University of Technology (Brisbane), The CSIRO Division = of Biomolecular Engineering (Melbourne), LaTrobe University Schools of Biochemistry and Electrical Engineering (Melbourne), The Kolling Institut= e of Medical Research (Sydney) and four of Australia's leading biotechnolog= y and diagnostic development companies: Agen Biomedical Ltd (Brisbane), Bioclone Australia Pty Ltd (Sydney), PanBio (Brisbane) and Silenus Laboratories Ltd (Melbourne). The positions described above are available at the Queensland University = of Technology in sunny Brisbane, Queensland. All enquiries for further deta= ils on research positions should be directed to the Secretary Ms Patricia = Dunn (E-mail t.dunn@qut.edu.au) and prospective Hons and PhD students should contact Prof. James Dale (E-mail j.dale@qut.edu.au). Prospective PhD students must contact Ms Michelle Foley (E-mail m.foley@qut.edu.au) to obtain application forms for APA or QUTPRA scolarships - note the deadlin= e is 31 October, 1995. Research applications should quote the relevant reference number and incl= ude evidence of academic qualifications and experience plus the names, postal and E-mail addresses, telephone and facsimile numbers of three referees. Applicants applying for more than one position must submit separate applications. Applications should reach the Human Resources Director, = QUT, Locked Bag No 2, Red Hill 4059 Brisbane Australia by the nominated closin= g date. *************************************************************************= ****** Ronald J. Epping BSc(ANU); PhD Biochemistry(ANU) Lecturer in Biochemistry/Molecular Biology Education Program Leader, CRC for Diagnostic Technologies SnailMail: School of Life Science, Queensland University of Technolog= y. G.P.O. Box 2434 Brisbane Queensland AUSTRALIA 4001 Telephone: + 61 7 3864 2576 Facsimile: + 61 7 3864 1534 E-mail: r.epping@qut.edu.au *************************************************************************= ****** *************************************************************************= *** Ronald J. Epping BSc(ANU); PhD Biochemistry(ANU) Lecturer in Biochemistry/Molecular Biology Education Program Leader, CRC for Diagnostic Technologies (CDT) SnailMail: School of Life Science, Queensland University of Technolog= y. G.P.O. Box 2434 Brisbane Queensland AUSTRALIA 4001 Telephone: + 61 7 3864 2576 Facsimile: + 61 7 3864 1534 E-mail: r.epping@qut.edu.au *************************************************************************= *** Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!UVA.PCMAIL.VIRGINIA.EDU!mgk2r From: mgk2r@UVA.PCMAIL.VIRGINIA.EDU ("Michael G. Kurilla") Newsgroups: bionet.diagnostics Subject: Re: Serological Controls in Test Kits Date: 2 Nov 1995 14:27:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511022225.RAA06270@uva.pcmail.Virginia.EDU> NNTP-Posting-Host: net.bio.net On Nov 2, 2:14pm, MHughes77@aol.com wrote: > Subject: Serological Controls in Test Kits > To fellow Diagnostics Newsgroup members: > > I am a scientist at a small diagnostics company involved in developing rapid, > easy-to-use tests designed to be used primarily in doctors' clinics or > veterinary practices for diagnosing a wide variety of infectious diseases, as > well as for screening donated blood for infectious agents. Our kits come > with ready-to-use reagents in dropper bottles and have good stability for > 12-18 months at 4 degrees C and 3-6 months at room temperature. We would > like to be able to offer human serological controls for some of the test > kits, preferably with the same desirable stability characteristics. > > Perhaps some of you would have some information or suggestions regarding the > following questions we have: > > 1. What is the best way to inactivate viruses without appreciably affecting > the antibody and/or antigen activity? There are probably many ways to do this. Psoralen is one method that is fairly specific for nucleic acid and protein sparing. At least tests with virally infected cells have not shown loss of antibody reactivity or T cell proliferative changes after treamtent. From owner-diagnostics@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!interactive.net!usenet From: schneip@interactive.net (Paul Schneider) Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal,bit.med.mxdiag-l,sci.engr.biomed,sci.med,sci.med.immunology Subject: CE Mark for Medical Devices Sold in Europe Date: Thu, 02 Nov 1995 21:12:37 GMT Organization: Interactive Networks Lines: 28 Distribution: world Expires: 12/01/95 Message-ID: <47bc9e$k45@ruby.interactive.net> Reply-To: schneip@interactive.net NNTP-Posting-Host: onyx.interactive.net Keywords: CE mark, European Economic Community X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.diagnostics:375 bionet.diagnostics.prenatal:63 sci.engr.biomed:4735 sci.med:99091 sci.med.immunology:2870 Could one of the UK readers of these newsgroups provide me by e-mail or posting to these newsgroups a copy of the Guidance Notes For Manufacturers seking to comply with the EC Directive for Medical Devices CE Mark Status issued by the Medical Device Agency. One of my clients has written to the agency at it's Russell Square, London address requesting the item and has received no reply to date. I realize that it may yet arrive, but knowing the red tape involved and the liklihood that it went by surface mail, I decided to try this newsgroup route. If you send me the document by e-mail please post that fact to these newsgroups so that my mail box doesn't get inundated with replys ;-). Thanks in advance for any help. BTW does the MCA have a presence on the Intenet? What about the EC divisions or committees involved with Medical Directives? Paul Schneider Paul Schneider, Ph.D., RAC |Paul Schneider,Ph.D.& Associates schneip@interactive.net | Regulatory Consultants 74604.2211@compuserve.com | Serving the Needs of the 548 Stevens Avenue | Health Care Industry Ridgewood, NJ 07450 |Tel:201-652-0706 FAX:201-652-1289 From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!mbdj From: mbdj@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Re: Microtitre plate PCR Date: 3 Nov 1995 01:43:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511030941.JAA03276@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net > We are looking at transferring some of our PCR assays to a microtitre = > plate format. Does anyone have any experience of this that they can share= > ? > > For example, is this method less robust than using microtubes? What sort = > of throughput gains did you achieve? What sort of microtitre plate/therma= > l cycler would you recommend - or not, as the case may be. All tips/hint= > s about how to make a succesful tranfer would be appreciated. > > Kevin > We've played around with this idea as well. From talking to various people we decided that the best solution was microtubes in a 96 well format (from Advanced Biotechnologies) that could be sealed with a rubber mat in the Perkin Elmer 9600. This works very well and there is no need to change anything. By all accounts the problem with microtitre plates is getting a uniform temperature across the plate. From a discussion I had with an Anachem rep once he seemed to think that there were people working on better plastic formulations that would solve this problem. If you are looking for particularly high through puts Anachems 384 well Q plates would be ideal, but only if they improve the plastic. If I were to do this sort of thing alot I would also purchase a 0.5 - 10 ul multichannel pipette, it would make life just that little more bearable. D.A.C. Jones SCRI Virology Dundee DD2 5DA Tel 01382 562731 email mbdj@scri.sari.ac.uk From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.sprintlink.net!newshub.cts.com!usenet From: b3748@cts.com (Bryan Kiehl) Newsgroups: bionet.cellbio,bionet.diagnostics,bionet.immunology Subject: Re: Risk Factor Date: Fri, 03 Nov 1995 03:09:18 GMT Organization: CTS Network Services Lines: 17 Message-ID: <47c0sp$eda@news2.cts.com> References: NNTP-Posting-Host: kiehl.cts.com X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.diagnostics:378 bionet.immunology:6087 Richard Schifreen wrote: >A colleague is trying to follow-up on a report he heard that a lipid >related risk marker has been documented that correlates closely with >lifespan. Can anyone suggest a source or reference? The only thing that >comes to mind is the relationship of apolipoproteins to artherosclerotic >disease and now Alzheimers. >Rich Schifreen phone: 301-840-4163 >Life Technologies fax: 301-670-1493 > E-Mail: rschifre@access.digex.net You've got it. The relationships was recently discussed in the Wall Street Journal. From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!newshub.cts.com!usenet From: b3748@cts.com (Bryan Kiehl) Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal,bit.med.mxdiag-l,sci.engr.biomed,sci.med,sci.med.immunology Subject: Re: CE Mark for Medical Devices Sold in Europe Date: Fri, 03 Nov 1995 03:07:22 GMT Organization: CTS Network Services Lines: 32 Message-ID: <47c0p5$eda@news2.cts.com> References: <47bc9e$k45@ruby.interactive.net> NNTP-Posting-Host: kiehl.cts.com X-Newsreader: Forte Free Agent 1.0.82 Xref: biosci bionet.diagnostics:377 bionet.diagnostics.prenatal:64 sci.engr.biomed:4749 sci.med:99156 sci.med.immunology:2879 schneip@interactive.net (Paul Schneider) wrote: >Could one of the UK readers of these newsgroups provide me by e-mail >or posting to these newsgroups a copy of the Guidance Notes For >Manufacturers seking to comply with the EC Directive for Medical >Devices CE Mark Status issued by the Medical Device Agency. One of my >clients has written to the agency at it's Russell Square, London >address requesting the item and has received no reply to date. I >realize that it may yet arrive, but knowing the red tape involved and >the liklihood that it went by surface mail, I decided to try this >newsgroup route. If you send me the document by e-mail please post >that fact to these newsgroups so that my mail box doesn't get >inundated with replys ;-). >Thanks in advance for any help. BTW does the MCA have a presence on >the Intenet? What about the EC divisions or committees involved with >Medical Directives? >Paul Schneider >Paul Schneider, Ph.D., RAC |Paul Schneider,Ph.D.& Associates >schneip@interactive.net | Regulatory Consultants >74604.2211@compuserve.com | Serving the Needs of the >548 Stevens Avenue | Health Care Industry >Ridgewood, NJ 07450 |Tel:201-652-0706 FAX:201-652-1289 This is an interesting topic. I work in a company that makes in vitro diagnostic devices. It is my understanding that we do not need to be ISO certified to obtain a CE mark, but do need to register. Can anyone discuss this topic for diagnostic lab tests? From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!COMPUSERVE.COM!100023.3435 From: 100023.3435@COMPUSERVE.COM ("J. H. MACKENZIE") Newsgroups: bionet.diagnostics Subject: Re: CE Mark for Medical Devices Sold in Europe Date: 3 Nov 1995 10:05:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 98 Sender: daemon@net.bio.net Distribution: world Message-ID: <951103180210_100023.3435_JHB58-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net In reply to messages regarding CE Mark for Medical Devices Sold in Europe: 1. Guidance documents on the medical device directive and CE marking are available but run into 100 + pages and are not suitable for e-mail. 2. Diagnostics companies do not have to comply with the MDD but will have to comply with the 'in-vitro diagnostics medical device directive' when it becomes law. (A draft has been recently published.) 3. My company specialise in assisting companies world wide with compliance to such directives and to ISO 9000. Details are as follows: Whether you are a designer and manufacturer or a distributor of medical devices you need to keep abreast of the myriad of legislative measures which affect the industry. Do you need ISO 9000 registration? How have the Standards changed? Is TickIT registration required for your software? What about new FDA GMP Regulations? How will the EU Directives Affect you? Medical Devices Directive? In-vitro Diagnostics Directive? Do you need the CE mark on your products? Are your instruments affected by EMC and low voltage Directives? If you have concerns about any or all of the above then M C S's QualiMedd service is designed to help you. M C S M C S is an independent U K based consultancy established early in 1990 and working within the areas of quality improvement specialising in Clinical Instrumentation, In-Vitro Diagnostics, Healthcare, Bio-Technology and Medical Devices in both manufacturing and service companies. QualiMedd is a specialist service specifically designed to assist with quality and regulatory issues within the healthcare industry. Experienced in the requirements of both manufacturers and customers, M C S offers technical guidance to clients in all areas from the explanation of requirements through preparation of detailed plans for regulatory systems, to assisting with the documentation and implementation of processes and procedures, culminating in final registration of systems and products to the required standards. Throughout the process the emphasis is firmly on quality and clients are kept closely informed and involved at each stage. Current M C S clients include companies ranging from a staff of under ten to companies employing several thousand Worldwide. M C S is currently working in the following industries: Medical / Analytical Equipment and In-vitro Diagnostics, Information Technology, Bio Technology, Medical Devices and Genetic Engineering. Programmes are individually tailored for each client with the emphasis being on ensuring that activities comply with International Standards. Although a full programme is offered, clients can use our services as little or as much as desired throughout the run-up to product or company registration and our team of experienced and qualified consultants are available to assist at all stages. JIM MACKENZIE Jim Mackenzie, the principal of M C S , has over twenty years experience in the health care industry and has developed an in-depth knowledge of medical and diagnostic regulations and instrumentation with several major corporations both at home and abroad. Jim's career started as an engineer with Technicon Instruments where he subsequently held several technical and management posts, both at home and overseas, before moving into senior management with American Monitor Corporation in Europe. Jim was also Corporate Instrumentation Manager for Serono Diagnostics before starting out as a consultant by setting up MCS early in 1990. Being a qualified bio-medical engineer and both a Quality Systems lead auditor and a TickIT software lead auditor, Jim Mackenzie is ideally placed to provide an unrivalled support service to all sectors of the healthcare industry, whether they be designers and manufacturers or end users. Jim is a long standing member of The Association For Services Management, a member of the Institute of Quality Assurance and is a recognised authority on all aspects of customer service and Quality Management within high technology industries. For further details on how the M C S QualiMedd service can help your products and your business please complete the attached card or contact us directly: MACKENZIE CONSULTING SERVICES LONG LANE HAWTHORN HILL MAIDENHEAD BERKSHIRE SL6 3TA ENGLAND, U.K. Telephone: (44) - (0)1628 - 782537 Facsimile: (44) - (0)1628 - 782537 From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!COMPUSERVE.COM!100273.165 From: 100273.165@COMPUSERVE.COM (Philip Shaw) Newsgroups: bionet.diagnostics Subject: (none) Date: 3 Nov 1995 09:42:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <951103172306_100273.165_EHV97-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net Thierry Leclipteux asked: I ave problem in keeping stable monoclonal antibody-peroxidase conjugate at 4 0C. Is there any chemicals able to protect their activity. I do not want to keep them at -20 0C in glycerol. Thanks Try calling Dr Tim Gibson, ULIS Biotechnology, Leeds, UK. telephone +44-113-233-2599 they specialise in this type of problem. Philip Shaw Biodot Ltd From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!cdc2.cdc.net!news1.cris.com!news From: wick@cris.com Newsgroups: bionet.diagnostics Subject: Buffer for HRP-Streptavidin Date: 3 Nov 1995 15:49:32 GMT Organization: Molecular Biology Resources, Inc. Lines: 15 Message-ID: <47ddmc$igl@spectator.cris.com> NNTP-Posting-Host: crc5-fddi.cris.com X-Newsreader: AIR News 3.X (SPRY, Inc.) Can anyone recommend a storage and dilution buffer for Horse Radish Peroxiase-Streptavidin conjugate? I would prefer to store the product in a liquid form at 4C if possible. Jim Wick E-mail: wick@cris.com Phone: 414-871-7199 FAX: 414-871-1273 Snail Mail: 5520 W. Burleigh St. Milwaukee, WI 53210 "Don't precipitate more than you can resuspend" From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: CE Mark for Medical Devices Sold in Europe Date: 3 Nov 1995 06:15:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <47bc9e$k45@ruby.interactive.net> NNTP-Posting-Host: net.bio.net > > Could one of the UK readers of these newsgroups provide me by e-mail > or posting to these newsgroups a copy of the Guidance Notes For > Manufacturers seking to comply with the EC Directive for Medical > Devices CE Mark Status issued by the Medical Device Agency. One of my > clients has written to the agency at it's Russell Square, London > address requesting the item and has received no reply to date. > Thanks in advance for any help. BTW does the MCA have a presence on > the Intenet? What about the EC divisions or committees involved with > Medical Directives? > Paul, I can't help with your specific query but I can tell you that there is = a lot of EC information available on the internet. There is a very good clearing = house at: http://www.tep.e-technik.tu-muenchen.de/~anzinger/en/euro-govt.html This site not only has links for the various Euro-bodies but also links = for all of the individual European state's government agencies. Hope this is helpful, Kevin > > Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Belgium.EU.net!chaos.kulnet.kuleuven.ac.be!aix1.segi.ulg.ac.be!parasito2.fmv.ulg.ac.be!thierry From: Thierry Leclipteux Newsgroups: bionet.diagnostics Subject: Immunoconjugate stabilizer Date: 3 Nov 1995 11:58:11 GMT Organization: Universite de Liege (Belgium) Lines: 4 Distribution: world Message-ID: <47d04j$1ph0@aix1.segi.ulg.ac.be> NNTP-Posting-Host: parasito2.fmv.ulg.ac.be X-Newsreader: Nuntius Version 1.2 X-XXMessage-ID: X-XXDate: Fri, 3 Nov 1995 10:59:56 GMT I ave problem in keeping stable monoclonal antibody-peroxidase conjugate at 4 °C. Is there any chemicals able to protect their activity. I do not want to keep them at -20 °C in glycerol. Thanks From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Belgium.EU.net!chaos.kulnet.kuleuven.ac.be!aix1.segi.ulg.ac.be!parasito2.fmv.ulg.ac.be!thierry From: Thierry Leclipteux Newsgroups: bionet.diagnostics Subject: Re: Serological Controls in Test Kits Date: 3 Nov 1995 11:54:26 GMT Organization: Universite de Liege (Belgium) Lines: 16 Distribution: world Message-ID: <47cvti$1ph0@aix1.segi.ulg.ac.be> References: <951102140530_96176626@emout04.mail.aol.com> NNTP-Posting-Host: parasito2.fmv.ulg.ac.be X-Newsreader: Nuntius Version 1.2 X-XXMessage-ID: X-XXDate: Fri, 3 Nov 1995 10:56:11 GMT In article <951102140530_96176626@emout04.mail.aol.com> , MHughes77@aol.com writes: I know several way to inactivate viruses. But probably those giving best results to protect the antigenicity would be : ß-propiolactone and UV radiation. I have to mention that Aujesky virus is not inactivate by ß-propioloactone but well by UV. It seems that there is no fixed recipe. So do some trials. I guess that (for human, field at least) control sera must be proven free of pathogens. I have to check in my guidelines to know what about it. As I am also working (part-time) for a diagnostic company in the vet field, I would like to know if there is any components to stabilize peroxidase conjugate in a liquid form in order to keep them either concentrated or ready-to-use in the kits. From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: IMPORTANT: BIOSCI miniFAQ Date: 3 Nov 1995 02:01:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511031000.CAA11783@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!uwm.edu!math.ohio-state.edu!magnus.acs.ohio-state.edu!csn!news-2.csn.net!usenet From: "David S. Dodd" Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal,bit.med.mxdiag-l,sci.engr.biomed,sci.med,sci.med.immunology Subject: Re: CE Mark for Medical Devices Sold in Europe Date: 3 Nov 1995 19:11:35 GMT Organization: Virtual Internet Presence, Inc. Lines: 8 Message-ID: <47dph7$rru@news-2.csn.net> References: <47bc9e$k45@ruby.interactive.net> NNTP-Posting-Host: 204.131.233.43 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; PPC) To: schneip@interactive.net X-URL: news://news.csn.net/47bc9e$k45@ruby.interactive.net Xref: biosci bionet.diagnostics:387 bionet.diagnostics.prenatal:65 sci.engr.biomed:4755 sci.med:99208 sci.med.immunology:2885 Dear Paul, Please contact O'Logic,who should be able to help you with your enquiries,at: http://www.Ologic.com/ Regards J.Dodd pp.David Dodd[Webmaster,O'Logic} From owner-diagnostics@net.bio.net Fri Nov 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!solaris.cc.vt.edu!homer.alpha.net!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!duckter.glaxo.med.ualberta.ca!user From: tyr-2@bones.biochem.ualberta.ca (Karl Fischer) Newsgroups: bionet.diagnostics Subject: Re: HSV with RT-PCR Date: Sat, 04 Nov 1995 00:11:34 +0100 Organization: Med. Microbiology, Univ. of Alberta Lines: 18 Distribution: world Message-ID: References: <199511012009.NAA06359@bock.ucs.ualberta.ca> NNTP-Posting-Host: duckter.glaxo.med.ualberta.ca X-Newsreader: Value-Added NewsWatcher 2.0b27.1+ In article <199511012009.NAA06359@bock.ucs.ualberta.ca>, Michele.Hales@UAlberta.CA (Michele Hales) wrote: > When looking for HSV (A DNA virus) she has read published articles saying to > run RT-PCR. Once you get the PCR product are there any other > protocols/procedures that need to be done? Unless the source of the specimen contained cells which may harbour viral RNA, it seems to me that the RT phase (and the specimen processing leading to it) would be unecessary for detection of the virus by PCR. Karl -- Karl Fischer tyr-2@bones.biochem.ualberta.ca From owner-diagnostics@net.bio.net Fri Nov 03 22:00:00 1995 Path: biosci!TECHNET.SG!pohyam From: pohyam@TECHNET.SG (Lim Pohyam) Newsgroups: bionet.diagnostics Subject: precipitation problems Date: 4 Nov 1995 08:30:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511041628.AAA05390@einstein.technet.sg> NNTP-Posting-Host: net.bio.net My bacterial recombinant proteins precipitates upon long storage at -20C. Upon thawing from freezer, I detected strands of precipitates. There is difficulty to re-suspend these strands. As a result, when I set up my microtiter plates, the activity is decreased. I sometimes encounter as much as 15-20% drop in protein concentration. This is creating havoc in my assays. I would like to receive feedback on how to remove this problem. what can I do? I keep losing my protein everytime I thawed them from the freezer! From owner-diagnostics@net.bio.net Fri Nov 03 22:00:00 1995 Path: biosci!UVA.PCMAIL.VIRGINIA.EDU!mgk2r From: mgk2r@UVA.PCMAIL.VIRGINIA.EDU ("Michael G. Kurilla") Newsgroups: bionet.diagnostics Subject: Re: precipitation problems Date: 4 Nov 1995 10:13:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511041811.NAA08539@uva.pcmail.Virginia.EDU> NNTP-Posting-Host: net.bio.net On Nov 4, 11:33am, Lim Pohyam wrote: > Subject: precipitation problems > My bacterial recombinant proteins precipitates upon long storage at -20C. > Upon thawing from freezer, I detected strands of precipitates. There is > difficulty to re-suspend these strands. As a result, when I set up my > microtiter plates, the activity is decreased. I sometimes encounter as much > as 15-20% drop in protein concentration. This is creating havoc in my > assays. I would like to receive feedback on how to remove this problem. > what can I do? I keep losing my protein everytime I thawed them from the > freezer! > > > -- End of excerpt from Lim Pohyam Freezing at -20C does not in reality always freeze. Anything dissolved in water will depress its freezing point. What can happen with proteins, particularly concentrated solutions is that as the solution freezes, the protein will tend to migrate to the remaining fluid regions and gradually become superconcentrated. Salts and buffers will also concentrate and the salt conditions or the pH can be drastically altered. In fact, at -20C, the proteins are not really frozen, rather there is a thin shell of fluid with concentrated salts and buffers. This can promote denturation or degradation of the protein over long term storage. Several options would include 1) freezing and storage at -70C 2) quick freezing in LN2 followed by -20C storage 3) adding glycerol to prevent freezing completely and storing at -20C Mike Kurilla From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!btnet!zetnet.co.uk!dispatch.news.demon.net!demon!intergen.demon.co.uk!angus From: angus@intergen.demon.co.uk (Angus Cameron) Newsgroups: bionet.diagnostics Subject: test Date: Mon, 6 Nov 1995 00:05:24 Organization: Intergen Lines: 2 Message-ID: NNTP-Posting-Host: intergen.demon.co.uk X-NNTP-Posting-Host: intergen.demon.co.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Advertising on bionet Date: 6 Nov 1995 03:44:44 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Can I make the point (again) that direct advertising of services, whether= those of MCS, O'Logic or someone supplying 'correction for naughty boys'= is not allowed on bionet, because of the nature of it's funding. Consult= the FAQ at http://www.bio.net if you are in any doubts. Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" Alasdair = Gray Scottish Agricultural Science Agency Edinburgh odonnell@sasa.gov.uk From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!chi-news.cic.net!simtel!swidir.switch.ch!scsing.switch.ch!news.dfn.de!RRZ.Uni-Koeln.DE!news.rhrz.uni-bonn.de!news.rhrz.uni-bonn.de!root From: root@mibi03.meb.uni-bonn.de (SuperUser) Newsgroups: bionet.diagnostics Subject: Q: Antiserum against C. difficile toxin commercially available? Date: 05 Nov 1995 14:05:07 GMT Organization: University of Bonn, Germany Lines: 14 Distribution: world Message-ID: Reply-To: ernst.molitor@uni-bonn.de NNTP-Posting-Host: mibi03.meb.uni-bonn.de Dear fellow netters, we have been using an antiserum to C. sordellii which showed cross-reactivity against the C. difficile toxin in a test procedure aimed at the detection of the latter organism's toxin. Unluckily, the product has been canceled by our former supplier, and we are running out of stock. I'd very much appreciate a hint on a possible source of the kind of antiserum needed for the assay... Regards, Ernst --- ernst.molitor@uni-bonn.de From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!btnet!zetnet.co.uk!dispatch.news.demon.net!demon!intergen.demon.co.uk!angus From: angus@intergen.demon.co.uk (Angus Cameron) Newsgroups: bionet.diagnostics Subject: A key sales/management position exists in Europe Date: Mon, 6 Nov 1995 00:17:53 Organization: Intergen Lines: 4 Message-ID: NNTP-Posting-Host: intergen.demon.co.uk X-NNTP-Posting-Host: intergen.demon.co.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Main US manufacturer of enabling raw materials for the diagnostic and biopharm industries is looking for a senior sales manager to develop their business in German speaking Europe. From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!MAIL.CIP.ORG.EC!velasco From: velasco@MAIL.CIP.ORG.EC Newsgroups: bionet.diagnostics Subject: (none) Date: 6 Nov 1995 09:17:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511060918.AA00950@mail.cip.org.ec> subscribe velasco@cip.org.ec -- Andres Velasco Centro Internacional de la Papa Casilla 17-16-129-CEQ Quito, Ecuador Telf. +593-2-690990, Internet: VELASCO@cip.org.ec From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: precipitation problems Date: 6 Nov 1995 08:40:14 -0800 Organization: Scottish Agricultural College Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511061639.IAA01961@net.bio.net> > Date: Sun, 05 Nov 95 01:47:35 > From: > Subject: precipitation problems > To: mx%"diagnost@net.bio.net" > Cc: > Return-Path: > Received: from festival.ed.ac.uk by SAC001.ed.sac.ac.uk (MX V4.1 AXP) with > SMTP; Sun, 05 Nov 1995 01:47:34 GMT > Received: from net.bio.net by festival.ed.ac.uk id aa06266; 5 Nov 95 1:46 GMT > Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA21816 for > diagnost-list; Sat, 4 Nov 1995 08:30:19 -0800 > Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id IAA21812 for > diagnost-arpanet; Sat, 4 Nov 1995 08:30:17 -0800 > To: diagnost@net.bio.net > From: Lim Pohyam > Subject: precipitation problems > Date: 4 Nov 1995 08:30:10 -0800 > Sender: daemon@net.bio.net > Message-ID: <199511041628.AAA05390@einstein.technet.sg> > NNTP-Posting-Host: net.bio.net > > My bacterial recombinant proteins precipitates upon long storage at -20C. > Upon thawing from freezer, I detected strands of precipitates. There is > difficulty to re-suspend these strands. As a result, when I set up my > microtiter plates, the activity is decreased. I sometimes encounter as much > as 15-20% drop in protein concentration. This is creating havoc in my > assays. I would like to receive feedback on how to remove this problem. what > can I do? I keep losing my protein everytime I thawed them from the freezer! > > > > Have you tried freeze-drying your proteins and redissolving from these? Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!BORG.EVMS.EDU!JL From: JL@BORG.EVMS.EDU ("Janos Luka, Ph.D.") Newsgroups: bionet.diagnostics Subject: proficiency testing Date: 6 Nov 1995 11:49:43 -0800 Organization: Eastern Virginia Medical School Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <781064D0462@borg.evms.edu> NNTP-Posting-Host: net.bio.net We are doing PCR testing for the following viruses: HSV-1, HSV-2, VZV, EBV, CMV, HHV-6, HIV (DNA and RNA), HCV. I would be interested to get contact with laboratories with similar PCR panels to exchange samples for proficiency testing. Anybody interested, please contact me by e-mail or phone. Janos Luka, Ph.D. Associate Professor Department of Pathology EVMS, Norfolk, VA USA e-mail: JL@BORG.EVMS.EDU phone: (804) 446-7917 From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!EU.net!ieunet!news.tcd.ie!bioftp.unibas.ch!daresbury!sunsite.doc.ic.ac.uk!warwick!bham!usenet From: Gabriel Landini Newsgroups: bionet.diagnostics Subject: NEW BOOK: FUTURE HEALTH Date: Tue, 07 Nov 1995 10:17:39 -0800 Organization: The University of Birmingham, UK. Lines: 24 Message-ID: <309FA2C3.367F@bham.ac.uk> NNTP-Posting-Host: dial1.bham.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1 (Windows; I; 16bit) New book! Pickover, C. (1995) FUTURE HEALTH, Computers and Medicine in the Twenty-First Century. St. Martin's Press: New York. ISBN 0-312-12602-6. This collection considers the tremendous effects that computers will have on medicine and medical service in the next century. The book also gives a sampling of state-of-the-art application of computers in medicine. The chapters describe: o futuristic operating rooms o the challenges of future medical schools in preparing 21st-century physicians o futuristic fractal models in pathology o the use of new medical imaging technologies o the use of electronic gophers to obtain medical information o digital dentistry o the use of artificial intelligence in medical diagnosis o computer conferencing for medical consulting o bloodless robotic surgery o making solid models from medical images o futuristic examinations rooms and much more.... From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dish.news.pipex.net!pipex!lade.news.pipex.net!pipex!sasa.gov.uk!news From: ransome@sasa.gov.uk (Karl Ransome) Subject: Re: Problem with news from SASA - please respond Organization: Scottish Agricultural Science Agency Date: Tue, 7 Nov 1995 13:42:38 GMT Message-ID: X-Newsreader: WinVN 0.91.3 References: Sender: news@sasa.gov.uk (Usenet) Lines: 13 In article , ransome@sasa.gov.uk (Karl Ransome) says: > >Some of my users are reporting that their news articles are not >being posted to this newsgroup. Thank you so much everybody for replying to my message so promptly which I posted this morning. This will help us to pinpoint our newsreader problem. We have had six useful replies. Thanks again. Karl Ian Ransome ransome@sasa.gov.uk System Administrator / Network Manager: SCO UNIX, OpenVMS, SunOS, Cromix, Novell, PC-NFS, Windows, DOS. Scottish Agricultural Science Agency, Edinburgh, EH12 8NJ, Scotland. From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rscreager@aol.com (RSCreager) Newsgroups: bionet.diagnostics Subject: Industrial post-docs Date: 7 Nov 1995 07:30:23 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 13 Sender: root@newsbf02.news.aol.com Message-ID: <47njgv$ich@newsbf02.news.aol.com> Reply-To: rscreager@aol.com (RSCreager) NNTP-Posting-Host: newsbf02.mail.aol.com We're thinking of establishing an Industrial Post-doctoral program at my company. I know this concept has been tried at a number of pharmaceutical and diagnostic companies. I would be grateful to hear from anyone who experienced such a program and their likes, dislikes, and overall opinions. Thanks. Richard S. Creager, Ph.D. Vice President, R&D Sanofi Diagnostics Pasteur Chaska, MN. rscreager@aol.com Richard S. Creager, Ph.D. Richard S. Creager, Ph.D. From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Re: Problem with news from SASA - please respond Organization: Scottish Agricultural Science Agency Date: Tue, 7 Nov 1995 11:29:17 GMT Message-ID: X-Newsreader: WinVN 0.91.6 References: Sender: news@sasa.gov.uk (Usenet) Lines: 5 This one was received OK Karl. Apologies to everyone else for this interruption. Kevin From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!sasa.gov.uk!news From: ransome@sasa.gov.uk (Karl Ransome) Subject: Problem with news from SASA - please respond Organization: Scottish Agricultural Science Agency Date: Tue, 7 Nov 1995 09:54:48 GMT Message-ID: X-Newsreader: WinVN 0.91.3 Sender: news@sasa.gov.uk (Usenet) Lines: 15 Some of my users are reporting that their news articles are not being posted to this newsgroup. My news articles to other newsgroups are being posted OK and I get replies to them very quickly from people in the UK and USA which shows the news is appearing on news readers outwith our establishment. If you are able to read this then I would appreciate a few people responding so that I can ascertain that the news to this group is getting through. Then I can try and work out why Kevin O'Donnell's articles are not appearing here. Karl Ian Ransome ransome@sasa.gov.uk System Administrator / Network Manager: SCO UNIX, OpenVMS, SunOS, Cromix, Novell, PC-NFS, Windows, DOS. Scottish Agricultural Science Agency, Edinburgh, EH12 8NJ, Scotland. From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.dra.com!news.getnet.com!news.dgsys.com!kpl From: Mblank@kpl.com (Martin Blankfard) Newsgroups: bionet.diagnostics Subject: Re: Buffer for HRP-Streptavidin Date: 7 Nov 1995 00:46:20 GMT Organization: KPL Lines: 10 Message-ID: <47m9i3$q6k_002@dgs.dgsys.com> References: <47ddmc$igl@spectator.cris.com> NNTP-Posting-Host: kpl.com X-Newsreader: News Xpress Version 1.0 Beta #4 >Can anyone recommend a storage and dilution buffer for Horse Radish Peroxiase-Streptavidin conjugate? I would prefer to store >the product in a liquid form at 4C if possible. > >I use 50% glycerol in RO water. Martin Blankfard KPL From owner-diagnostics@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.dra.com!news.getnet.com!news.dgsys.com!kpl From: Mblank@kpl.com (Martin Blankfard) Newsgroups: bionet.diagnostics Subject: Re: Immunoconjugate stabilizer Date: 7 Nov 1995 00:53:14 GMT Organization: KPL Lines: 19 Message-ID: <47m9up$q6k_003@dgs.dgsys.com> References: <47d04j$1ph0@aix1.segi.ulg.ac.be> NNTP-Posting-Host: kpl.com X-Newsreader: News Xpress Version 1.0 Beta #4 In article <47d04j$1ph0@aix1.segi.ulg.ac.be>, Thierry Leclipteux wrote: >I ave problem in keeping stable monoclonal antibody-peroxidase conjugate >at 4 °C. Is there any chemicals able to protect their activity. I do not >want to keep them at -20 °C in glycerol. >Thanks If you are going to keep them at -20 you should be able to in PBS. 50% glycerol will keep them from going through freeze/thaws and that seems to be death for most HRP conjugates in our hands. I have also found fish Gel. at 1 mg/ml to work well. Best of luck Martin Balnkfard Senior Scientist Kirkegaard and Perry Laboratories 2 Cessna Ct. Gaithersburg Md , 20879 Phone: (US) 1-800-638-3167 x141 FAX : 301 948 9442 From owner-diagnostics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!crocus.mmid.med.ualberta.ca!user From: mdiadio@gpu.srv.ualberta.ca (Margo Diadio) Newsgroups: bionet.diagnostics Subject: Re: Problem with news from SASA - please respond Date: 7 Nov 1995 21:01:56 GMT Organization: University of Alberta - Medical Micro & Immunology Lines: 21 Message-ID: References: NNTP-Posting-Host: crocus.mmid.med.ualberta.ca In article , ransome@sasa.gov.uk (Karl Ransome) wrote: > Some of my users are reporting that their news articles are not > being posted to this newsgroup. My news articles to other > newsgroups are being posted OK and I get replies to them very > quickly from people in the UK and USA which shows the news is > appearing on news readers outwith our establishment. > > If you are able to read this then I would appreciate a few > people responding so that I can ascertain that the news to this > group is getting through. Then I can try and work out why > Kevin O'Donnell's articles are not appearing here. > Your message has been posted to this newsgroup through the U of A server in Edmonton. > Karl Ian Ransome ransome@sasa.gov.uk > System Administrator / Network Manager: > SCO UNIX, OpenVMS, SunOS, Cromix, Novell, PC-NFS, Windows, DOS. > Scottish Agricultural Science Agency, Edinburgh, EH12 8NJ, Scotland. From owner-diagnostics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!earth.superlink.net!usenet From: G-Shock Newsgroups: bionet.diagnostics Subject: (no subject) Date: Sun, 05 Nov 1995 06:25:25 -0500 Organization: SuperNet Inc. (908) 828-8988 Lines: 6 Message-ID: <309C9F25.7C17@mars.superlink.net> NNTP-Posting-Host: sb29.superlink.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1 (Windows; I; 32bit) CC: glenalan@mars.superlink.net Anyone know of any Net sources for info regarding Regulatory topics with respect to manufacture of medical devices (IVDs specifically). ie cGMPs, ISO requirements, new CFR releases, etc... Thanks for any info, Glen From owner-diagnostics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!agresearch.cri.nz!HartA From: HartA@agresearch.cri.nz Newsgroups: bionet.diagnostics Subject: Glycogen Date: 8 Nov 1995 11:36:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HXFLDJI4KQ001OXK@INVERMAY.CRI.NZ> NNTP-Posting-Host: net.bio.net Dear Diagnostics Members, Does anyone have a protocol,or a reference, for determination of glycogen in animal tissue (muscle)? On re-reading the diagnostics charter, I note that it is primarily for serological or nucleic acid-based techniques. Does anyone know of newsgroups for general biochemical analyses? I'd also be interested in any for, or including, chemical and biosensors. Thanks, Alan Hart. From owner-diagnostics@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!ieunet!news.tcd.ie!bioftp.unibas.ch!citi2.fr!jussieu.fr!univ-lyon1.fr!rockendo.univ-lyon1.fr!pcomm From: pcomm@cismibm.univ-lyon1.fr (Pierre Commercon) Newsgroups: bionet.diagnostics Subject: PAPPA Antibody Date: Wed, 8 Nov 1995 15:10:22 Organization: Universite Lyon1 Pharmacie Lines: 10 Message-ID: NNTP-Posting-Host: rockendo.univ-lyon1.fr X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hello I need a monoclonal or polyclonal antibody to Pregnancy Associated Plasma Protein A. I know the Dako one, and I need an other one Thanks in advance Congratulations Pcomm@cismibm.univ-lyon1.fr From owner-diagnostics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!COMPUSERVE.COM!100416.735 From: 100416.735@COMPUSERVE.COM (The Diagnostics Club) Newsgroups: bionet.diagnostics Subject: CE Mark, ISO approvals and info sources Date: 9 Nov 1995 07:18:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <951109151326_100416.735_BHG98-1@CompuServe.COM> NNTP-Posting-Host: net.bio.net Regarding 'G-Shocks query, the following message, which was sent directly to Paul Schneider in response to his query about CE Mark, is relevant. ********************** Re: your note about no feedback from the Medical Devices Agency. Possible reason is that they have moved New address is: Hannibal House, Elephant & Castle, London SE1 6TQ, UK tel: +44 171 972 8000 email: mda_mail@mda.win-uk.net European Directives contact point tel: +44 171 972 8300, fax: +44 171 972 8112 Best of luck, but get back to us if you still hit problems **************** The offer of further help applied to all. Tony Owen Diagnostics Club Administrator tel: +44 1908 647417, fax: +44 1908 271612 From owner-diagnostics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.sprintlink.net!news.internet-eireann.ie!usenet From: info@biotrin.ie (Conor Byrne) Newsgroups: bionet.diagnostics Subject: Anti- 'Bovine IgE' Abs wanted Date: 8 Nov 1995 17:46:26 GMT Organization: Biotrin International Ltd. Lines: 7 Message-ID: <47qqdi$2nh@server1.internet-eireann.ie> Reply-To: info@biotrin.ie NNTP-Posting-Host: bhett.biotrin.ie X-Newsreader: WinVN 0.92.6+ Does anybody know the name of any manufacturers/distributors who produce anti-'bovine IgE' monoclonal abs. I would appreciate any relevant info. Thanks in advance, Conor Byrne, info@biotrin.ie http://www.internet-eireann.ie/Biotrin/ From owner-diagnostics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!news3.noc.netcom.net!ix.netcom.com!netnews From: Cubano67.ix.netcom.com Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: Thu, 09 Nov 1995 01:12:06 GMT Organization: Netcom Lines: 32 Message-ID: <47rk9q$lot@ixnews6.ix.netcom.com> References: <199511011139.DAA18368@net.bio.net> NNTP-Posting-Host: ix-sd8-23.ix.netcom.com X-NETCOM-Date: Wed Nov 08 5:08:10 PM PST 1995 X-Newsreader: Forte Free Agent 1.0.82 ESA009@ED.SAC.AC.UK ("Dr Rob Harling") wrote: >We're using RAPD-PCR to study diversity in epiphytic Pseudomonas >species. We prepare the template DNA using Flowgen's Puregene kit, >which is basically an SDS cell lysis, ammonium acetate protein >precipitation, RNAse, isopropanol DNA precipitation, then dissolve in >water. The resulting DNA is of good spectrophotometric quality. >PCR works if we use freshly - prepared DNA, ie extracted from the >bacteria then run on the same day. If we store the DNA at 4C for >only one week, then PCR fails, we get zilch in the lanes at any >concentration of template. The DNA still looks the same after one week >when run on a gel, ie there doesn't seem to be nuclease breakdown >of the DNA. Does anyone know what's happening to the DNA in storage? What is your storage buffer (if any) and why do you store at 4C? >Thanks >Rob (still got my hair) Harling >Dr Rob Harling >SAC (Scottish Agricultural College)/ > University of Edinburgh >West Mains Road >Edinburgh EH9 3JG >Scotland, UK >tel: +44 (0)131 535 4000 >fax: +44 (0)131 667 2601 >e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!aol.com!MHughes77 From: MHughes77@aol.com Newsgroups: bionet.diagnostics Subject: Antibodies against Plasmodium falciparum Date: 9 Nov 1995 14:55:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <951109175255_82808957@emout06.mail.aol.com> NNTP-Posting-Host: net.bio.net Diagnostics Newsgroup members: I am seeking a supplier of polyclonal anti-Plasmodium antibodies. I also desire a monoclonal antibody against Plasmodium falciparum conjugated to gold (approx. 20 nm). Any help would be greatly appreciated. Mark Hughes From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!biosci!not-for-mail From: Simon Eaton Newsgroups: bionet.announce,bionet.diagnostics,bionet.metabolic-reg,bionet.general Subject: 3rd Shire Workshop Date: 9 Nov 1995 21:36:43 -0800 Organization: Newcastle University, UK Lines: 33 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: <47silf$s8c@whitbeck.ncl.ac.uk> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.announce:2613 bionet.diagnostics:414 bionet.metabolic-reg:609 bionet.general:18398 3rd SHIRE WORKSHOP Mitochondrial Genetics and Disease 11th December 1995 Lecture Theatre The Sir James Spence Institute of Child Health The Royal Victoria Infirmary Newcastle upon Tyne NE1 4LP PROGRAMME 2.00 - 2.10pm Introduction and welcome, Professor A W Craft 2.10 - 2.40pm Basic mitochondrial genetics, Dr RN Lightowlers, Lecturer in Neurology, University of Newcastle 2.40 - 3.20pm Clinical features of mitochondrial disease, Dr AAM Morris, Institute of Child Health, University of London 3.20 - 3.40pm Tea/Coffee 3.40 - 4.10pm Mitochondrial abnormalities: Their relationship to ageing Dr E J Brierley, Lecturer in Geriatrics University of Newcastle 4.10 - 4.45pm The diagnosis and treatment of patients with mitochondrial DNA disorders DM Turnbull, Professor of Clinical Neuroscience, University of Newcastle 4.45 - 5.00pm Questions and close _______________________________________________________________ Interested in coming? E-mail to Anne.Kennedy@ncl.ac.uk _______________________________________________________________ From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news.internet-eireann.ie!usenet From: info@biotrin.ie (Conor Byrne) Newsgroups: bionet.diagnostics Subject: Correction re: Anti-'bovine IgE' abs wanted Date: 10 Nov 1995 14:25:25 GMT Organization: Biotrin International Ltd. Lines: 10 Message-ID: <47vncl$8jk@server1.internet-eireann.ie> Reply-To: info@biotrin.ie NNTP-Posting-Host: bhett.biotrin.ie X-Newsreader: WinVN 0.92.6+ I wish to post a correction to my last posting inquiring about anti-'bovine IgE' monoclonal abs. I would be grateful if anyone could give me the names/addresses of any reseachers/groups who may be using anti-'bovine IgE' monoclonals, not just manufacturers Once again, thanks in advance Conor Byrne email: info@biotrin.ie http://www.internet.eireann.ie/Biotrin/ From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: 10 Nov 1995 06:25:42 -0800 Organization: Scottish Agricultural College Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511101417.GAA00106@net.bio.net> NNTP-Posting-Host: net.bio.net > Subject: Re: now you see it now you don't > To: mx%"diagnost@net.bio.net" > ESA009@ED.SAC.AC.UK ("Dr Rob Harling") wrote: > > >We're using RAPD-PCR to study diversity in epiphytic Pseudomonas > >species. We prepare the template DNA using Flowgen's Puregene kit, > >which is basically an SDS cell lysis, ammonium acetate protein > >precipitation, RNAse, isopropanol DNA precipitation, then dissolve in > >water. The resulting DNA is of good spectrophotometric quality. > > >PCR works if we use freshly - prepared DNA, ie extracted from the > >bacteria then run on the same day. If we store the DNA at 4C for > >only one week, then PCR fails, we get zilch in the lanes at any > >concentration of template. The DNA still looks the same after one week > >when run on a gel, ie there doesn't seem to be nuclease breakdown > >of the DNA. Does anyone know what's happening to the DNA in storage? > On Thu, 09 Nov 95 17:47:16 asked: "What is your storage buffer (if any) and why do you store at 4C?" I use water for storage (because TE might mess up my carefully calibrated PCR reactions) and I store at 4C because I continually take out samples for conducting PCR and do not wish to freeze/thaw. Thanks for your response. Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!bangslabs.com!leigh From: leigh@bangslabs.com ("Leigh") Newsgroups: bionet.diagnostics Subject: FWD>RE>Dyed Particles Paten Date: 10 Nov 1995 06:14:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net FWD>RE>Dyed Particles Patents 10/30/95 We know that many companies sell immunochromatographic strip tests using gold and dyed microspheres (latex particles). Does anyone know what patents limit their widespread use? I know of a Unipath patent in Europe (May, K., M.E. Prior, and I. Richards, Immunoassays and Devices Therefor, WO 88/08534 International Patent, 1988, November 3.), but none in the US (apparently). There is a Becton-Dickinson patent in the US (Campbell, R., D. Wagner, J. O'Connell, Solid Phase Assay with Visual Readout, US 4,703,017. 1987, Oct. 27.). These are all I know about. Please advise any others which are pertinent. Leigh Bangs, aka "The Particle Doctor"; leigh@bangslabs.com Bangs Laboratories, Inc., 979 Keystone Way, Carmel, IN 46032-2823 USA Tel: 317-844-7176 Fax: 317-575-8801 "The Microsphere Zone" [Web Home Page: http://www.bangslabs.com/blab] From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!UVA.PCMAIL.VIRGINIA.EDU!mgk2r From: mgk2r@UVA.PCMAIL.VIRGINIA.EDU ("Michael G. Kurilla") Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: 10 Nov 1995 07:30:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511101528.KAA27200@uva.pcmail.Virginia.EDU> NNTP-Posting-Host: net.bio.net On Nov 10, 9:30am, "Dr Rob Harling" wrote: > Subject: Re: now you see it now you don't > > > Subject: Re: now you see it now you don't > > To: mx%"diagnost@net.bio.net" > > > > > ESA009@ED.SAC.AC.UK ("Dr Rob Harling") wrote: > > > > >We're using RAPD-PCR to study diversity in epiphytic Pseudomonas > > >species. We prepare the template DNA using Flowgen's Puregene kit, > > >which is basically an SDS cell lysis, ammonium acetate protein > > >precipitation, RNAse, isopropanol DNA precipitation, then dissolve in > > >water. The resulting DNA is of good spectrophotometric quality. > > > > >PCR works if we use freshly - prepared DNA, ie extracted from the > > >bacteria then run on the same day. If we store the DNA at 4C for > > >only one week, then PCR fails, we get zilch in the lanes at any > > >concentration of template. The DNA still looks the same after one week > > >when run on a gel, ie there doesn't seem to be nuclease breakdown > > >of the DNA. Does anyone know what's happening to the DNA in storage? > > > On Thu, 09 Nov 95 17:47:16 > asked: > > "What is your storage buffer (if any) and why do you store at 4C?" > > I use water for storage (because TE might mess up my carefully > calibrated PCR reactions) and I store at 4C because I continually > take out samples for conducting PCR and do not wish to freeze/thaw. > Thanks for your response. The rationale for TE as a storage buffer is straightforward. The EDTA chelates Mg that all nucleases require. That doesn't seem to be your problem (although it could develop over time if your go in and out of that tube repeatedly. The Tris (pH8.0) is where DNA is most stable. Basic solution promote strand breakage (again not your problem). Acid pH can be bad because of protonation of some of the bases. This can lead to ring cleavage that would certainly cause PCR headaches. Check the pH of your water. Even ultrapure H2O tends to be a little on the acidic side. Why not prepare a weak storag buffer, like tris-HCl 1.0mM at pH8.0? Mike Kurilla From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!WADSWORTH.ORG!jack.woodall From: jack.woodall@WADSWORTH.ORG Newsgroups: bionet.diagnostics Subject: ProMED - the Program for Monitoring Emerging Diseases Date: 10 Nov 1995 07:03:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 71 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511101502.KAA08578@wadsworth.ph.albany.edu> NNTP-Posting-Host: net.bio.net ProMED - THE PROGRAM FOR MONITORING EMERGING DISEASES ===================================================== *Sponsored by the Federation of American Scientists* *Distributed by SatelLife* A central goal of ProMED is to establish a direct partnership among scientists concerned with emerging infectious diseases in all parts of the world; building the appropriate networks to encourage communicating and sharing information is a key objective. Reporting of incidents or outbreaks, infectious disease problems of emerging interest, and discussions on how to improve surveillance and response capabilities (including needs at the local level) are especially encouraged. ProMED invites and welcomes the participation of all interested colleagues. To subscribe to the ProMED electronic conference (which is moderated), send e-mail to: majordomo@usa.healthnet.org Leave the Subject line blank (or put anything you like there), and write subscribe promed in the text space. You will receive an automatic reply with information on how to access past files. From then on, you will receive the messages posted to the ProMED conference as they are received, and can post messages to the network. If you prefer to receive a digest of several messages together (approx. once per day), instead of multiple daily messages, make that message subscribe promed-digest If you would like to receive ONLY emerging disease reports (EDR), with no discussion, announcements, reviews etc., make that message subscribe promed-edr You can cancel these at any time by sending unsubscribe promed or unsubscribe promed-digest or unsubscribe promed-edr We welcome your participation in ProMED, and invite your support of ProMED's mission to develop and promote effective early warning systems for emerging infections. -- For further information on ProMED: For questions on the ProMED Dr. Stephen S. Morse electronic network: Chair, ProMED Dr. Jack Woodall The Rockefeller University ProMED List Moderator <1230 York Avenue, Box 120 NYS Department of Health New York, NY 10021-6399 Albany, NY 12201 E-mail: morse@rockvax.rockefeller.edu E-mail: woodall@wadsworth.org =======================================end================================== -- Jack Woodall, ProMED List Moderator, New York State Dept.of Health, Albany NY, USA e-mail: jack.woodall@wadsworth.org From owner-diagnostics@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: Re: now you see it now you don't Date: 10 Nov 1995 07:03:04 -0800 Organization: Scottish Agricultural College Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511101459.GAA02268@net.bio.net> NNTP-Posting-Host: net.bio.net > Subject: Re: now you see it now you don't > To: mx%"diagnost@net.bio.net" > ESA009@ED.SAC.AC.UK ("Dr Rob Harling") wrote: > > >We're using RAPD-PCR to study diversity in epiphytic Pseudomonas > >species. We prepare the template DNA using Flowgen's Puregene kit, > >which is basically an SDS cell lysis, ammonium acetate protein > >precipitation, RNAse, isopropanol DNA precipitation, then dissolve in > >water. The resulting DNA is of good spectrophotometric quality. > > >PCR works if we use freshly - prepared DNA, ie extracted from the > >bacteria then run on the same day. If we store the DNA at 4C for > >only one week, then PCR fails, we get zilch in the lanes at any > >concentration of template. The DNA still looks the same after one week > >when run on a gel, ie there doesn't seem to be nuclease breakdown > >of the DNA. Does anyone know what's happening to the DNA in storage? > On Thu, 09 Nov 95 17:47:16 asked: "What is your storage buffer (if any) and why do you store at 4C?" I use water for storage (because TE might mess up my carefully calibrated PCR reactions) and I store at 4C because I continually take out samples for conducting PCR and do not wish to freeze/thaw. Thanks for your response. Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Fri Nov 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.interlog.com!winternet.com!interactive.net!usenet From: schneip@interactive.net (Paul Schneider) Newsgroups: bionet.diagnostics Subject: Re: (no subject) Date: Sat, 11 Nov 1995 20:53:34 GMT Organization: InterActive Networks, Inc. - Paterson, NJ Lines: 24 Message-ID: <4832lt$ppf@ruby.interactive.net> References: <309C9F25.7C17@mars.superlink.net> NNTP-Posting-Host: onyx.interactive.net X-Newsreader: Forte Free Agent 1.0.82 G-Shock wrote: >Anyone know of any Net sources for info regarding Regulatory topics with respect to >manufacture of medical devices (IVDs specifically). ie cGMPs, ISO requirements, new CFR >releases, etc... >Thanks for any info, >Glen Try the FDA BBS , which you can access through Fed World. I don't have the URL handy but you should be able to get what you need through YAHOO or some other search site. Paul S. Paul Schneider, Ph.D., RAC |Paul Schneider,Ph.D.& Associates schneip@interactive.net | Regulatory Consultants 74604.2211@compuserve.com | Serving the Needs of the 548 Stevens Avenue | Health Care Industry Ridgewood, NJ 07450 |Tel:201-652-0706 FAX:201-652-1289 From owner-diagnostics@net.bio.net Sun Nov 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!lll-winken.llnl.gov!simtel!harbinger.cc.monash.edu.au!usenet From: Rob Willis Newsgroups: bionet.diagnostics Subject: Dariers Disease (Keratosis follicularis) Date: Mon, 13 Nov 1995 12:32:00 +1000 Organization: Monash University Lines: 4 Message-ID: <30A6AE20.384E@bs.monash.edu.au> NNTP-Posting-Host: hux.bs.monash.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1J (Windows; I; 32bit) Any information on the latest treatments for Dariers Disease (Keratosis follicularis) in children? Email: rwillis@bs.monash.edu.au From owner-diagnostics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!zetnet.co.uk!demon!intergen.demon.co.uk!angus From: angus@intergen.demon.co.uk (Angus Cameron) Newsgroups: bionet.diagnostics Subject: Repost of Senior Sales vacancy Date: Tue, 14 Nov 1995 10:09:45 Organization: Intergen Lines: 5 Message-ID: NNTP-Posting-Host: intergen.demon.co.uk Summary: A sales vacancy exists Keywords: diagnostics biopharmaceutical german X-NNTP-Posting-Host: intergen.demon.co.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Still seeking a senior sales manager to control a business in Europe working to supply diagnostic and biopharmaceutical manufacturers, must have German speaking skills and an intimate knowledge of the businesses. Can be located in Germany or the UK, but preferably UK near Oxford. From owner-diagnostics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!demon!user From: filter@firthcom.demon.co.uk (Steve Firth) Newsgroups: bionet.diagnostics Subject: Re: Candida albicans Date: Tue, 14 Nov 1995 12:36:50 +0000 Organization: Firth Consulting Lines: 19 Distribution: bionet Message-ID: References: <489vh8$65n@mserv1.dl.ac.uk> NNTP-Posting-Host: firthcom.demon.co.uk X-NNTP-Posting-Host: firthcom.demon.co.uk In article <489vh8$65n@mserv1.dl.ac.uk>, Pete Kwasowski wrote: >I am looking for information on suppliers of ELISA kits for the detection of >antibodies to Candida albicans. Try Centocor Diagnostics (formerly Mercia Diagnostics), who have a vey good test kit for C. albicans. 01483 505255 The company is based in Shalford, just down the road from you. -- Steve Firth . Idiot: A member of a large and powerful tribe steve@firthcom.demon.co.uk . whose influence in human affairs has always Random sigs for Internet . been dominant and controlling. Config from sigFried 0.1a . From owner-diagnostics@net.bio.net Mon Nov 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!btnet!zetnet.co.uk!demon!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Pete Kwasowski Newsgroups: bionet.diagnostics Subject: Candida albicans Date: 14 Nov 1995 11:45:44 -0000 Lines: 25 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <489vh8$65n@mserv1.dl.ac.uk> X-Sender: bss3pk@central.surrey.ac.uk Original-To: diagnost@dl.ac.uk I am looking for information on suppliers of ELISA kits for the detection of antibodies to Candida albicans. Failing that, suggestions on a source of antigen would be useful. Many thanks in advance, Pete Kwasowski Dr P Kwasowski School Biological Sciences University of Surrey Guildford Surrey GU2 5XH United Kingdom Tel/FAX: +44 (0)1483 257908 E-mail: P.Kwasowski@surrey.ac.uk http://www.surrey.ac.uk/SBS/comps/clifmar.html _\\|//_ (-0-0-) /----------------------------------ooO-(_)-Ooo-------------------------------\ From owner-diagnostics@net.bio.net Thu Nov 16 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!cs.utexas.edu!usc!elroy.jpl.nasa.gov!swrinde!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!uknet!newsfeed.ed.ac.uk!leeds.ac.uk!news From: wilsonpj@eng.derby.ac.uk (Peter Wilson) Subject: Gene Screening X-URL: news:bionet.diagnostics Message-ID: <1995Nov17.165608.12138@leeds.ac.uk> X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.4 sun4c) Content-Type: text/plain; charset=us-ascii MIME-Version: 1.0 Date: Fri, 17 Nov 1995 16:56:08 +0000 (GMT) Lines: 10 Content-Transfer-Encoding: 7bit I am looking for any information on the problems with gene screeing as I have to do a seminare on this in two weeks so any information would be much appreciated. Please could you email me as I do not have acess to these newsgroups all the time. Thankyou inadvance Peter Wilson Wilsonpj@eng.derby.ac.uk From owner-diagnostics@net.bio.net Sun Nov 19 22:00:00 1995 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!simtel!news00.sunet.se!sunic!news99.sunet.se!news.funet.fi!news.cc.tut.fi!slip-pc21 From: trpede@uta.fi (Pentti Degerstr|m) Newsgroups: bionet.diagnostics Subject: Loss of hair, sense of smell, and sense of taste Date: 20 Nov 1995 22:32:19 GMT Organization: Tampere University of Technology, Computing Centre Lines: 26 Distribution: world Message-ID: <48qvlj$suq@cc.tut.fi> NNTP-Posting-Host: slip-pc21.uta.fi X-Newsreader: News Xpress Version 1.0 Beta #4 HELP, PLEASE! My sister (30 years) is going bald. She has been losing her hair for three years, and no new hair has grown back to substitute for the loss. Her thyroid gland hormone secretion is normal. My mother (60 years) has lost her olfaction and her threshold for sense of taste has become higher after she had a common cold. Her sense of smell has been non-existent for a year now. Her brain was scanned but no tumour was found. What could be behind these syndromes? Is there any treatment? (Could laser therapy help and what kinds of laser therapy exist?) Any comments would be greatly appreciated. P.D. From owner-diagnostics@net.bio.net Mon Nov 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!swrinde!sdd.hp.com!vixen.cso.uiuc.edu!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!amd!netcomsv!uu4news.netcom.com!netcomsv!uu3news.netcom.com!ix.netcom.com!netnews From: t-bone13@ix.netcom.com (Dan Tisone ) Newsgroups: bionet.diagnostics.prenatal,bionet.diagnostics Subject: New web site - Equipment for diagnostic test kits Date: 21 Nov 1995 01:58:08 GMT Organization: Netcom Lines: 9 Message-ID: <48rbng$76k@ixnews7.ix.netcom.com> NNTP-Posting-Host: ix-ir14-26.ix.netcom.com X-NETCOM-Date: Mon Nov 20 5:58:08 PM PST 1995 Xref: biosci bionet.diagnostics.prenatal:73 bionet.diagnostics:429 I would like to announce a new web site for those who are interested: http://www.lainet.com/~biodot (coming soon http://www.biodot.com) The company at this site specializes in equipment for the manufacture and production of rapid diagnostic, ELISA type, and assay type test kits. From owner-diagnostics@net.bio.net Wed Nov 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!elroy.jpl.nasa.gov!lll-winken.llnl.gov!simtel!torn!nott!ottawanet!usenet From: David and Gail Gall Newsgroups: bionet.diagnostics.prenatal,bionet.diagnostics Subject: Re: New web site - Equipment for diagnostic test kits Date: 23 Nov 1995 01:29:52 GMT Organization: Internet Access Inc. <613> 225-5595 Lines: 16 Message-ID: <490iqg$5nt@news.ottawa.net> References: <48rbng$76k@ixnews7.ix.netcom.com> NNTP-Posting-Host: slip-ppp35.ottawa.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: gall@em.agr.ca Xref: biosci bionet.diagnostics.prenatal:76 bionet.diagnostics:431 -- David and Gail Gall galldg@ottawa.net -- |====================== Access Provided By: =========================| | Internet Access Inc. voice (613) 225-5595 info: info@ottawa.net | | Providing all levels of access to the internet. web: www.ottawa.net | |======================================================================== =| From owner-diagnostics@net.bio.net Wed Nov 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!elroy.jpl.nasa.gov!lll-winken.llnl.gov!simtel!torn!nott!ottawanet!usenet From: David and Gail Gall Newsgroups: bionet.diagnostics.prenatal,bionet.diagnostics Subject: Re: New web site - Equipment for diagnostic test kits Date: 23 Nov 1995 01:30:28 GMT Organization: Internet Access Inc. <613> 225-5595 Lines: 26 Message-ID: <490irk$5nt@news.ottawa.net> References: <48rbng$76k@ixnews7.ix.netcom.com> NNTP-Posting-Host: slip-ppp35.ottawa.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: nielsenk@em.agr.ca Xref: biosci bionet.diagnostics.prenatal:77 bionet.diagnostics:432 t-bone13@ix.netcom.com (Dan Tisone ) wrote: >I would like to announce a new web site for those who are interested: > >http://www.lainet.com/~biodot >(coming soon http://www.biodot.com) > >The company at this site specializes in equipment for the manufacture >and production of rapid diagnostic, ELISA type, and assay type test >kits. > -- David and Gail Gall galldg@ottawa.net -- |====================== Access Provided By: =========================| | Internet Access Inc. voice (613) 225-5595 info: info@ottawa.net | | Providing all levels of access to the internet. web: www.ottawa.net | |======================================================================== =| From owner-diagnostics@net.bio.net Wed Nov 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!elroy.jpl.nasa.gov!lll-winken.llnl.gov!simtel!torn!nott!ottawanet!usenet From: David and Gail Gall Newsgroups: bionet.diagnostics.prenatal,bionet.diagnostics Subject: Re: New web site - Equipment for diagnostic test kits Date: 23 Nov 1995 01:29:29 GMT Organization: Internet Access Inc. <613> 225-5595 Lines: 26 Message-ID: <490ipp$5nt@news.ottawa.net> References: <48rbng$76k@ixnews7.ix.netcom.com> NNTP-Posting-Host: slip-ppp35.ottawa.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: gall@em.agr.ca Xref: biosci bionet.diagnostics.prenatal:75 bionet.diagnostics:430 t-bone13@ix.netcom.com (Dan Tisone ) wrote: >I would like to announce a new web site for those who are interested: > >http://www.lainet.com/~biodot >(coming soon http://www.biodot.com) > >The company at this site specializes in equipment for the manufacture >and production of rapid diagnostic, ELISA type, and assay type test >kits. > -- David and Gail Gall galldg@ottawa.net -- |====================== Access Provided By: =========================| | Internet Access Inc. voice (613) 225-5595 info: info@ottawa.net | | Providing all levels of access to the internet. web: www.ottawa.net | |======================================================================== =| From owner-diagnostics@net.bio.net Sat Nov 25 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!pipeline!psinntp!psinntp!psinntp!interramp.com!usenet From: us011939@interramp.com Newsgroups: bionet.diagnostics,bionet.immunology,misc.health.alternative,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.pathology,sci.med.psychobiology Subject: HELP!!! CROHN'S IN THE STOMACH!! Date: Sun, 26 Nov 95 19:23:30 GMT Organization: PSI Public Usenet Link Lines: 63 Message-ID: NNTP-Posting-Host: ip84.newark.nj.interramp.com X-Newsreader: Quarterdeck Message Center [1.0] Xref: biosci bionet.diagnostics:433 bionet.immunology:6369 misc.health.alternative:51331 sci.med.immunology:3144 sci.med.informatics:4304 sci.med.nursing:10679 sci.med.nutrition:33627 sci.med.pathology:1735 sci.med.psychobiology:9056 I NEED HELP OR ANY ADVICE...... It seems to be a puzzle that my doctors are not able to solve at this time. from Mark I am computer scientist capable of solving all sorts of problems. However to solve my most important problem, I feel completely helpless. Enough said.. My doctors have diagnosed me few years(3 1/2) ago with Crohns disease. For most of the time disease stayed in terminal ileum part of small intestine. This has been confirmed by bunch of medical tests and endoscopies. The symptoms that I used to have was loose bowel movements twice a week. A day before a major flare up, I used to feel exhausted,no energy or vigor. I also had difficulty breathing at times. I never had a bloody diarrhea. About 1 year ago, I felt a lot of pain the middle stomach. I also had a lot of acidity taste in the mouth and esophagus. Anyway, my doctors told me that they diagnosed crohn's ulcer in the stomach walls. However, they continue to tell me that it is very very minor. They put me on Pentasa (4x 4 times a day) and Prilosec(1 day). I don't have that acid taste anymore. It also helped a bit with the lower stomach(in the small intestine area). My bowel movements are pretty normal. But, I feel a lot more burning pain and sensation in upper and mid stomach area. Sometimes, I feel that my guts are bursting inside. The other times, I feel that someone is scratching my guts from inside. Help!!!!!! Also, about once a week, I don't seem to have any energy. I am completely exhausted. I find myself difficulty breathing normal air.I have a lot of pain in the eyes as well as toes and nails. Is there anything I could do? Could I take some other medicine. My doctors keep telling me that I don't need anything else. What about herbs? or vitamins(my vitamin tests were all normal). What I need to travel abroad to get any further medical treatment? Where? Thank you very much. Mark New Jersey,USA Please respond to this newsgroup or my private address us011939@interramp.com(stated also above) mark From owner-diagnostics@net.bio.net Sat Nov 25 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!chi-news.cic.net!news.uiowa.edu!red.weeg.uiowa.edu!bmayer From: "B. Mayer" Newsgroups: bionet.diagnostics,bionet.immunology,misc.health.alternative,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.pathology,sci.med.psychobiology Subject: Re: HELP!!! CROHN'S IN THE STOMACH!! Date: Sun, 26 Nov 1995 14:50:59 -0600 Organization: University of Iowa, Iowa City, IA, USA Lines: 91 Distribution: world Message-ID: References: NNTP-Posting-Host: red.weeg.uiowa.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: bmayer@red.weeg.uiowa.edu In-Reply-To: Xref: biosci bionet.diagnostics:434 bionet.immunology:6373 misc.health.alternative:51340 sci.med.immunology:3150 sci.med.informatics:4305 sci.med.nursing:10686 sci.med.nutrition:33634 sci.med.pathology:1737 sci.med.psychobiology:9058 First, there is a newsgroup: alt.support.crohn's-colitis is a group I read regularly and might be of some help. I have CD and am actually just recovering from surgery (resection of the terminal ilieum). Second, Crohn's in the stomach is VERY rare. How did your drs diagnose this? You never mentioned colonoscopy as a diagnostic tool but I don't know how else Crohn's of the terminal ilieum can be diagnosed. It needs to be biopsied to be confrimed. And that is how the stomach ulcer should be diagnosed. What medications were you on before the stomach pain appeared? I had terrible stomach pain this summer and it was a result of the meds that I take. I also have "significant" reflux, however, which is relatively common with Crohn's. I would strongly recommed a second opinion. Reflux can be treated with a variety of meds and if it is Crohn's in the stomach, there are other treatments that could be considered if this is interferring with you everyday life. Also, drop into alt.support.crohn's-colitis. Lots of information and lots of us with CD, UC and IBS. Good luck and let me know it turns out. Waiting for dx and treatment is so frustrating....I know. Brenda Mayer On Sun, 26 Nov 1995 us011939@interramp.com wrote: > I NEED HELP OR ANY ADVICE...... It seems to be a puzzle that my doctors are not > able to solve at this time. > > from Mark > > I am computer scientist capable of solving all sorts of problems. However to > solve my most important problem, I feel completely helpless. Enough said.. > > > My doctors have diagnosed me few years(3 1/2) ago with Crohns disease. For most > of the time disease stayed in terminal ileum part of small intestine. This has > been confirmed by bunch of medical tests and endoscopies. The symptoms that I > used to have was loose bowel movements twice a week. A day before a major flare > up, I used to feel exhausted,no energy or vigor. I also had difficulty > breathing at times. I never had a bloody diarrhea. > > About 1 year ago, I felt a lot of pain the middle stomach. I also had a lot of > acidity taste in the mouth and esophagus. Anyway, my doctors told me that they > diagnosed crohn's ulcer in the stomach walls. However, they continue to tell me > that it is very very minor. They put me on Pentasa (4x 4 times a day) and > Prilosec(1 day). I don't have that acid taste anymore. It also helped a bit > with the lower stomach(in the small intestine area). My bowel movements are > pretty normal. > > But, I feel a lot more burning pain and sensation in upper and mid stomach > area. Sometimes, I feel that my guts are bursting inside. The other times, I > feel that someone is scratching my guts from inside. Help!!!!!! > > Also, about once a week, I don't seem to have any energy. I am completely > exhausted. I find myself difficulty breathing normal air.I have a lot of pain > in the eyes as well as toes and nails. > > > Is there anything I could do? Could I take some other medicine. My doctors keep > telling me that I don't need anything else. What about herbs? or vitamins(my > vitamin tests were all normal). What I need to travel abroad to get any further > medical treatment? Where? > > Thank you very much. > > > Mark > > New Jersey,USA > Please respond to this newsgroup or my private address > us011939@interramp.com(stated also above) > > > > > > > > > > > > > > > > mark > > > From owner-diagnostics@net.bio.net Sun Nov 26 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: jamesr5923@aol.com (JamesR5923) Newsgroups: bionet.diagnostics,bionet.immunology,misc.health.alternative,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.pathology,sci.med.psychobiology Subject: Re: HELP!!! CROHN'S IN THE STOMACH!! Date: 26 Nov 1995 21:34:25 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <49b83h$60u@newsbf02.news.aol.com> References: Reply-To: jamesr5923@aol.com (JamesR5923) NNTP-Posting-Host: newsbf02.mail.aol.com Xref: biosci bionet.diagnostics:435 bionet.immunology:6378 misc.health.alternative:51376 sci.med.immunology:3158 sci.med.informatics:4308 sci.med.nursing:10695 sci.med.nutrition:33640 sci.med.pathology:1743 sci.med.psychobiology:9063 Try web server - http://qurlyjoe.bu.edu/ for information sources on Crohn's. God luck. Jim Robertson, Pharm.D. From owner-diagnostics@net.bio.net Sun Nov 26 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.iii.net!news From: wesley@price.iii.net (wesley price) Newsgroups: bionet.diagnostics,bionet.immunology,misc.health.alternative,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.pathology,sci.med.psychobiology Subject: Re: HELP!!! CROHN'S IN THE STOMACH!! Date: 27 Nov 1995 21:46:41 GMT Organization: iii.net subscriber Lines: 142 Message-ID: <49dbkk$bb4@news.iii.net> References: NNTP-Posting-Host: price.iii.net Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.7 Xref: biosci bionet.diagnostics:436 bionet.immunology:6391 misc.health.alternative:51465 sci.med.immunology:3173 sci.med.informatics:4317 sci.med.nursing:10731 sci.med.nutrition:33670 sci.med.pathology:1747 sci.med.psychobiology:9078 found this article surfing a couple of days ago ======================== A theory that Crohn's disease may be caused by swallowing toothpa first proposed about forty years ago, continues to surface from time to tim We became aware of this hypothesis when a doctor on a health news se television mentioned it. We learned later that he was referring to an article which had appeared in Lancet.

The reasoning behind the thesis is that abrasive substances found in tooth pastes or powders, particularly silica, have produced enteric lesions in rats and dogs given these crystalline products in food or by injection. These experiments had been done because the strikingly increased thickn the affected bowel wall and adjacent mesentery and lymph nodes sugges lymphatic blockage. Similar blockage was known to occur in the lymph no the lungs of silicosis sufferers. It was known, also, that the lesions or granulomas in the intestinal tracts of Crohn's patients are the same as tho in silicosis-afflicted lungs.

But--swallowing toothpaste? Children, especially, do ingest it, and perhaps everyone does, to a certain extent, in small quantities. However, what researchers seem not to know is there's a much larger source of sil much more widespread.

It's in the water. Some occurs naturally from streams passing over rock, some is added purposefully to reduce corrosivity. But the largest amount comes from fluosilicates used in modern water fluoridation progra This is the most dangerous source, for the fluoride part reacts with gastri secretions to form hydrofluoric acid, the only acid able to dissolve silica.

The combination of hydrofluoric acid and silica produces silicic acid, highly destructive to tissues and known to be the kind which causes lesions in the lungs when silicon dust is inhaled. Sixty-two per cent of the population of the United States drinks fluoridated water, most of it containing either hydrofluosilicic acid or sodium silicofluoride. They eat foods and consume beverages prepared or processed with the water. Th would contain more concentrated amounts of the substance. The rest of it from foods and beverages processed in fluoridated regions and exported other places, even to other countries. In addition, thirty-five nations in total have fluoridated their water supplies. To some extent, at least.

Silica has a trait that makes it hazardous if it stays in the system over time. Protein binds to it, making abnormal lesions and growths. This in turn, can cause the immune system to react. It was known in 1963 tha antibodies were being created against unidentified antigens in regional enteritis. Scientists today have found that antibodies are produced when silicone breast implants leak. (Silicone, like silica, is a compound of silicon.) It has also been found that children with silicone tubes implanted in their ears to drain fluids have developed antibodies against silicone in some cases.

Crohn's disease was relatively obscure--not recognized as a separa disease entity from other inflammatory bowel problems--before the 1930's Water systems weren't being fluoridated then--not officially. But hydrofluosilicic acid, a by-product of the phosphate fertilizer industry, was routinely dumped into the water before it became illegal to do so. Phosph fertilizer was actually first made in 1868 but it didn't come into wide usage until after World War I. That could account for the noted rise of this intestinal disease in the 1930's. After water fluoridation became an accepted public health measure, hydrofluosilicic acid was often the fluorid product of choice because it was already in solution, cheaper and easier t use than sodium fluoride. Apparently, no one thought of the possibility tha the silica content could prove to be a health risk, made so by the fluoride connection.

The pinpointing of the major source of silica should lead to a more scientific basis for the view that it's implicated in Crohn's disease. Knowing that it's not toothpaste could provide a more productive explanat of the cause, prevention, and possible cure of this distressing condition.

So why doesn't everyone have it, with silica being so prevalent? It may be genetic. The National Crohn's and Colitis Foundation has announ researchers have pinpointed Chromosome 6 as the "home" of gene mark Crohn's and ulcerative colitis. Dr. Jerome Rotter, a geneticist at Cedars-Sinai Medical Center, the leader of the team, says that about 3 billion pairs of genes are duplicated in every cell in the human body. To find unique genetic variations is "like looking at the Earth from a satellite and trying to zero in on a particular house. We've found the neighborhood...Now we're conducting a block-to-block search to find the specific markers."

We suggest that finding them won't lead to anything useful if scientists continue to ignore the substance to which Crohn's victims may genetically indisposed. A good many genetic diseases need such "trigger and without them wouldn't be led to serious consequences. For Wilson's disease, for example, it's copper; for PKU disease it's inability to handle certain proteins in the first year of life (but avoiding them prevents disastrous results); for Alzheimer's--though it hasn't been admitted yet!--it's likely to be aluminum, particularly in the form of aluminum fluoride. The point is, preventing contact with the offending substance is more important, in terms of a cure, than finding a particular "house" in a particular "neighborhood" as researchers spend their time doing.

Sincerely,
Frances Frech
fransfrech@aol.com

>Is there anything I could do? Could I take some other medicine. My doctors keep >telling me that I don't need anything else. What about herbs? or vitamins(my >vitamin tests were all normal). What I need to travel abroad to get any further >medical treatment? Where? > >Thank you very much. > > > Mark > >New Jersey,USA >Please respond to this newsgroup or my private address >us011939@interramp.com(stated also above) > > > > > > > > > > > > > > > >mark > From owner-diagnostics@net.bio.net Sun Nov 26 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!peer-news.britain.eu.net!xara.net!zynet.net!usenet From: Peter Jackman Newsgroups: bionet.diagnostics Subject: gel fingerprint analysis Date: Mon, 27 Nov 1995 22:06:21 -0800 Organization: Zynet Ltd Lines: 5 Message-ID: <30BAA6DD.7D0E@biosys.zynet.co.uk> NNTP-Posting-Host: biosys.zynet.co.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1 (Windows; I; 16bit) I am pleased to announce BioSystematica's WWW site URL: http://www.cis.plym.ac.uk/biosys/biosys.htm providing information on gel analysis software and systems From owner-diagnostics@net.bio.net Mon Nov 27 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!swrinde!howland.reston.ans.net!ix.netcom.com!netcom.com!sjscharf.slip.netcom.com!user From: scharfs@rnisd0.dnet.roche.com (Stephen Scharf) Subject: HLA B27 testing Message-ID: Sender: netnews@mork.netcom.com Nntp-Posting-Host: sjscharf.slip.netcom.com Organization: Roche Molecular Systems Date: Tue, 28 Nov 1995 17:37:42 GMT Lines: 14 Hi Netters, Does anyone here do any HLA-B27 testing for ankylosing spondylitis? I'm curious if anyone that does testing for this marker uses Antibodies and FACS or PCR. How important is it to test for various other HLA specifities, e.g. the cross- reactive group (B0701, 0702, 0703, 4201, 5401, 5501, 5502, 5601, 5602)? Any help would be most appreciated. Thanks, Stephen Scharf. -- Stephen J. Scharf email:sjscharf@netcom.com or: 72070,750@Compuserve.com From owner-diagnostics@net.bio.net Wed Nov 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!prodigy.com!usenet From: FZMP57A@prodigy.com (Kim Kerns) Newsgroups: bionet.diagnostics Subject: Getting Test Results Back to Patients Faster Date: 30 Nov 1995 05:10:24 GMT Organization: Prodigy Services Company 1-800-PRODIGY Lines: 188 Distribution: world Message-ID: <49jec0$ssg@usenetz1.news.prodigy.com> NNTP-Posting-Host: inugap4.news.prodigy.com X-Newsreader: Version 1.2 New Physician to Patient Voice Messaging Service Saves You and Your Staff Time and Money..... & Patients Get Test Results Faster !! MedConnect, now available in Canada and USA, shifts the total burden away from physicians of insuring that patients receive test results and places more of this responsibility with patients themselves. The MedConnect service bureau saves you large chucks of time. This is accomplished by assuring that every time you or your nurse picks up the phone to deliver a test result you will reach the patient's confidential voice mail box.. never a busy signal or discovering your patient is not at home.. MedConnect gives patients the convenience of picking up their secure messages, recorded in your or nurse's own voice, at the patient's convenience ( 24 hours a day - 7 days a week ). The physician to patient messaging is uni-directional (i.e. patients receive messages and can not leave them). Medconnect was designed in this manner to protect the your time. Medconnect also supports two way physician to physician voice messaging and is now available for a flat rate giving physicians throughout the USA and Canada a North American Voice Messaging Network. With the addition of International Callback Service, Medconnect can be inexpensively accessed throughout the entire world. You have complete flexibility in deciding when to use or not use the service on a patient by patient basis. Daily fax reports are generated informing you of patients who have not picked up their messages and complete detail reports of all messages left and retrieved are mailed to you at month end. Medconnect is purchased on a "delivered " message basis. Patient retrieval of messages are free. MedConnect runs on an AT&T Unix platform, is written in Scriptbuilder, has an Oracle database and uses a Merlin switch .. no one ever hears a busy signal and Medconnect provides the highest quality digitized voice playback of your voice available today! MedConnect is now operational and supporting physicians in all 50 states and many parts of Canada via toll free 800 numbers. The script on the next two pages demonstrates how easy it is to receive and record messages. This is an on line demonstration with live a 800 number and you are encouraged to try the service out yourself right now!. Example of How Patient would Listen to a Your Message: ________________________________________________ To Listen to a message.. dial 1-800-636-7978 Type in your social security number 069-38-7504 Type in your Test Number 1 # Other Examples: Please try these other 'Pin " numbers: 2# 3# 4# 5# 6# Listen to your message...... Your message remains on the system for five days after you first listened to it so that you may call back and hear it again! Perhaps the most powerful script has been left by family practice physician who has been using our service for some time ... To access these message please dial the same 800 number 1-800-636-7978.... but this time at the voice prompt type in 456-789-123 for the social security number, then at the voice prompt type in 99999#... Example of How Physician would deliver Message to Patient's Mail Box ______________________________________________ 1. Dial 1-800- 636-7978 2. Enter *8# 3. Enter your physician pass code 631079# 4. Enter " 1" to add a message for patient 1 " 2 " to record a (standard) default message " 3 " to record message for another physician " 4 " to leave a voice mail message ( reminder ) " 5 " to listen to your voice mail messages 5. Enter Patient's social security number ( enter your ss# ) 6. Enter the patient's pin 1# 7. Enter code to record a custom message 3 ( otherwise use code 1 or 2 to leave default message ) Example of message: _______________ Hello Mr. Black, this is Dr. Wilbern.. the results of your cholesterol test have arrived and I'm pleased to say that they are all within normal ranges. Please continue to stay on your low fat diet and we will re-check your count in two weeks. Please call us at 800-576-5146 to schedule an appointment. Good-bye.... see our Web Page at Http://netmar.com/medconnect MedConnect's Voice Messaging Price List Recorded Monthly fee Messages $100 up to100 msgs. One Time Set-up Fee / per Account $200 price per additional delivered message: (patient pick-up of messages are free) $1.00/msg * * rebates available for high volume accounts For More Information Call: 804-257-0372 Applied Information Technologies, Inc. 3001 River Hill Terrace Midlothian , VA 23113 For more information please call 804-257-0372 or send name and address to: Kim Kerns Applied Information Technologies, Inc. 3001 River Hill Terrace Midlothian, Va. 23113 e-mail kkerns@i2020.net Web Site: http://netmar.com/medconnect 804-257-0372 voice mail 804-378-8050 office 804-794-9659 fax From owner-diagnostics@net.bio.net Wed Nov 29 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk Newsgroups: bionet.diagnostics Subject: Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: 30 Nov 1995 03:57:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511301154.aa26483@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net > Attention PCR USERS > > I just found a great new product from Kalyx Biosciences. AMPLISCRIPT > > allows you to detect your PCR products using an ELISA format. This sounds interesting. Can you give a few more details about it? Does it involve specially labelled primers for example, or can plain PCR primers be used? Does it work on 96-well plates? Kevin Kevin O'Donnell SASA Edinburgh From owner-diagnostics@net.bio.net Thu Nov 30 22:00:00 1995 Path: biosci!TECHNET.SG!pohyam From: pohyam@TECHNET.SG (Lim Pohyam) Newsgroups: bionet.diagnostics Subject: PCR lab setup Date: 1 Dec 1995 07:48:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512011546.XAA12911@einstein.technet.sg> NNTP-Posting-Host: net.bio.net I need information on how to setup a PCR lab as a diagnostic tool in the medical field. Can anyone direct me to an information site or would share experience with me on their own setups?