From owner-diagnostics@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech2!EU.net!cph-1.news.DK.net!dkuug!dknet!icl.icl.dk!sw0198!news.icl.fi!news.eunet.fi!news.funet.fi!news.kbfi.ee!news.latnet.lv!tesla!afl From: Alexei Flerov Newsgroups: bionet.diagnostics,bionet.immunology,misc.health.alternative,sci.med.immunology,sci.med.informatics,sci.med.nursing,sci.med.nutrition,sci.med.pathology,sci.med.psychobiology Subject: Re: HELP!!! CROHN'S IN THE STOMACH!! Date: Sat, 2 Dec 1995 01:44:00 +0200 Organization: LatNet news site Lines: 72 Message-ID: References: NNTP-Posting-Host: tesla.sal.lv Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: afl@tesla cc: us011939@interramp.com In-Reply-To: Return-Receipt-To: afl@sal.lv Xref: biosci bionet.diagnostics:444 bionet.immunology:6478 misc.health.alternative:51930 sci.med.immunology:3246 sci.med.informatics:4373 sci.med.nursing:10930 sci.med.nutrition:33905 sci.med.pathology:1778 sci.med.psychobiology:9137 On Sun, 26 Nov 1995 us011939@interramp.com wrote: > I NEED HELP OR ANY ADVICE...... It seems to be a puzzle that my doctors are not > able to solve at this time. > > from Mark > > I am computer scientist capable of solving all sorts of problems. However to > solve my most important problem, I feel completely helpless. Enough said.. > > > My doctors have diagnosed me few years(3 1/2) ago with Crohns disease. For most > of the time disease stayed in terminal ileum part of small intestine. This has > been confirmed by bunch of medical tests and endoscopies. The symptoms that I > used to have was loose bowel movements twice a week. A day before a major flare > up, I used to feel exhausted,no energy or vigor. I also had difficulty > breathing at times. I never had a bloody diarrhea. > May be it helps: Subject: Testimonial #3 Intestinals From:Ward Hammond Life Plus products have changed my life. I have suffered with Crohn's disease since 1980. I've been in the hospital 4 or 5 times, and on and off medication. Three years ago I had a brain abcess (a possible side affect of Crohn's) but I lived. Last year I finally had to have surgery on my small intestine. The operation was successful but no matter what I did the diarrhea wouldn't stop. My doctor said my colon just had to adjust. No help. Then I heard about Pycnogenol. I listened to a tape and it mentioned that Pycnogenol was good for gastroenterological disorders like Crohn's, so I HAD to try it. At the time I placed my order they recommended L-Salivarius as well. Two days after taking the supplements the diarrhea was gone and I started having normal bowel movements once a day. You can't imagine what a God send this was untill you have gone through months and months of pain and running to the bathroom. Well I was sold on the products and have been taking them for months, but I didn't know how to market them! Until I joined the Kempf group. My upline, has been working hard to support me with information, encouragement, guidance, email follow-ups, and phone calls. In just a few days I signed up my first distributor and have several dozen likely prospects... after only one week! I can't say enough about the company, the products, my upline, the products, the compensation plan, the products, the ease of sponsoring... did I mention the products. I would order and take Life Plus products even if they cost 5 times as much and I didn't make a dime. That's how good they are. The money is just a bonus because without your health, money means nothing. I recommend the Synergy Pack, Info Pack and a bottle of L-Salivarius. You can thank me later. -=- Ward Hammond -=- "Suggested Nutritional Guide" recommends: CROHN'S DISEASE Somazyme, Proanthenols, Protx II For more info see: http://www.geopages.com/wallstreet/2270 From owner-diagnostics@net.bio.net Fri Dec 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: Dan Tisone Newsgroups: bionet.diagnostics Subject: Kalyx Biosciences Date: Sat, 02 Dec 1995 13:39:06 -0800 Organization: Netcom Lines: 4 Message-ID: <30C0C77A.755D@ix.netcom.com> NNTP-Posting-Host: ix-ir9-23.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Sat Dec 02 1:36:39 PM PST 1995 X-Mailer: Mozilla 2.0b2 (Windows; I; 32bit) Does anyone have an address and phone number for this company? Dan Tisone t-bone13@ix.netcom.com From owner-diagnostics@net.bio.net Sat Dec 02 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: IMPORTANT: BIOSCI miniFAQ Date: 3 Dec 1995 02:00:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512031000.CAA08075@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news.starnet.net!wupost!waikato!agresearch.cri.nz!collinr From: collinr@agresearch.cri.nz (Roger Collin) Newsgroups: bionet.diagnostics Subject: Re: Kalyx Biosciences Date: Tue, 5 Dec 1995 08:45:31 Organization: AgResearch Lines: 13 Message-ID: References: <30C0C77A.755D@ix.netcom.com> NNTP-Posting-Host: 160.4.128.189 X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] >Does anyone have an address and phone number for this company? >Dan Tisone >t-bone13@ix.netcom.com They have an eMail address: kalyx@magi.com R. G. Collin AgResearch New Zealand From owner-diagnostics@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!BORG.EVMS.EDU!JL From: JL@BORG.EVMS.EDU ("Janos Luka, Ph.D.") Newsgroups: bionet.diagnostics Subject: Re: Kalyx Biosciences Date: 4 Dec 1995 13:20:39 -0800 Organization: Eastern Virginia Medical School Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Does anyone have an address and phone number for this company? >Dan Tisone >t-bone13@ix.netcom.com The address is: Kalyx Biosciences Inc. 20 Camelot Drive Nepean, Ontario Canada K2G 5X8 phone (613) 723-1114 Janos Luka JL@Borg.evms.edu From owner-diagnostics@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!bull.hkstar.net!b2!alo From: alo@b2.hkstar.com (Lo Wing Ip Anthony) Newsgroups: bionet.diagnostics Subject: Re: PCR lab setup Date: 5 Dec 1995 03:11:50 GMT Organization: Hong Kong Star Internet Ltd. Lines: 26 Distribution: world Message-ID: <4a0d9m$oc@bull.hkstar.net> References: <199512011546.XAA12911@einstein.technet.sg> NNTP-Posting-Host: b2.hkstar.com X-Newsreader: TIN [version 1.2 PL2] Lim Pohyam (pohyam@TECHNET.SG) wrote: : I need information on how to setup a PCR lab as a diagnostic tool in the : medical field. Can anyone direct me to an information site or would share : experience with me on their own setups? Hi, Our hospital is also setting up a kind of diagnostic laboratory with molecular biology support. Which particular field are you interested in? Microbiology? Histopath? Chem Path? I start it off with a small bench which is far from the standard set up Farka et al. If you are interested, please e-mail me for further discussion. I am no expert but just someone also starting this up. Regards Lo Wing Ip Anthony MO Tel: (852)-2990-1870 Chemical Pathology Laboratory Fax: (852)-2370-0969 Princess Margaret Hospital E-mail: anthony-lo@cuhk.hk Lai King Hill, Kowloon. alo@hkstar.com Hong Kong. alo@gpu.srv.ualberta.ca From owner-diagnostics@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!torn!newshost.uwo.ca!usenet From: gwade@physiology.uwo.ca Newsgroups: bionet.diagnostics Subject: Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: Thu, 07 Dec 1995 22:30:11 GMT Organization: The University of Western Ontario, London, Ont. Canada Lines: 52 Message-ID: <4a7pls$cm6@falcon.ccs.uwo.ca> References: <9511301154.aa26483@jura.sasa.gov.uk> NNTP-Posting-Host: pc37.physiology.uwo.ca X-Newsreader: Forte Free Agent 1.0.82 odonnell@sasa.gov.uk wrote: >> Attention PCR USERS >> >> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT >> >> allows you to detect your PCR products using an ELISA format. >This sounds interesting. Can you give a few more details about it? Does it involve specially labelled >primers for example, or can plain PCR primers be used? Does it work >on 96-well plates? >Kevin >Kevin O'Donnell >SASA >Edinburgh No labelled primers. Works on 96 welled plates. Its actually quite a simple process involving using a T7 promoter region on your probe (primer). After pcr one will have generated multiple copies of the intended gene. The user then makes complementary RNA copies of this code (thanks to the T7 promoter now being on the amplified DNA from the original PCR). So now you have many copies of RNA which is complementary to the DNA you were probing for. Perform a nucleic acid hybridization with some control DNA and you get heteronucleic acid (hNA). Kalyx has antibodies to the hNA allowing one to determine the presence of this molecule. All the reagents required to do this (except TAQ) are provided. I hope the way I have explained it is possible to follow. Kalyx has some literature outlining this process. Interestingly enough they also have an inexpensive matrix on which you can do a nucleic acid hybridization in about 90 min (at room temp). good luck GREG WADE Physiology PH.D Candidate The University of Western Ontario London, Ontario Canada. From owner-diagnostics@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!MAGI.COM!eja From: eja@MAGI.COM Newsgroups: bionet.diagnostics Subject: (none) Date: 6 Dec 1995 17:26:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512070123.AA03102@magi.com> NNTP-Posting-Host: net.bio.net Subscribe diagnost eja@magi.com Thanks..Eng Au From owner-diagnostics@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!rug.ac.be!mario.vaneechoutte From: mario.vaneechoutte@rug.ac.be Newsgroups: bionet.diagnostics Subject: (Fwd) Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: 7 Dec 1995 23:17:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512080715.AB01155@allserv.rug.ac.be> NNTP-Posting-Host: net.bio.net >> Attention PCR USERS >> >> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT >> >> allows you to detect your PCR products using an ELISA format. >This sounds interesting. Can you give a few more details about it? Does it involve specially labelled >primers for example, or can plain PCR primers be used? Does it work >on 96-well plates? >Kevin > I contacted Kalyx immediately via email after reading the first posting of this interesting message, asking for more information and for addresses of local suppliers. No answer yet, which surprises me. Has anyone else tried to contact Kalyx? Mario Vaneechoutte From owner-diagnostics@net.bio.net Thu Dec 07 22:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!mbdj From: mbdj@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Re: (Fwd) Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: 8 Dec 1995 01:53:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512080949.JAA19528@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net > >> Attention PCR USERS > >> > >> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT > >> > >> allows you to detect your PCR products using an ELISA format. > > >This sounds interesting. Can you give a few more details about it? Does it involve specially labe> lled > >primers for example, or can plain PCR primers be used? Does it work > >on 96-well plates? > > >Kevin > > > > > I contacted Kalyx immediately via email after reading the first > posting of this interesting message, asking for more information and > for addresses of local suppliers. No answer yet, which surprises me. > Has anyone else tried to contact Kalyx? > > Mario Vaneechoutte > > I did exactly the same and I haven't recieved a reply yet. May be they have just been inundated and can't cope with the demand. Dave. From owner-diagnostics@net.bio.net Fri Dec 08 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!howland.reston.ans.net!newsfeed.internetmci.com!btnet!news.insnet.net!news.netkonect.net!linux.nildram.co.uk!usenet From: harbron@nildram.co.uk (Stuart Harbron) Newsgroups: bionet.diagnostics Subject: Enzyme Amplification for ImmunoAssays and GeneProbe Assays Date: Thu, 07 Dec 1995 16:55:23 GMT Organization: Nildram On-Line Lines: 8 Message-ID: <4a7b3q$pjg@linux.nildram.co.uk> NNTP-Posting-Host: ppp1.nildram.co.uk X-Newsreader: Forte Free Agent 1.0.82 I have just put a reasonably comprehensive description of the FADP-based enzyme amplification assay for alkaline phosphatase my colleagues and I have developed onto my home page at http://www.nildram.co.uk/~harbron. This assay can detect lesss than 0.1 amol in around 30 minutes, colorimetrically! Stuart Harbron From owner-diagnostics@net.bio.net Sat Dec 09 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!agate!howland.reston.ans.net!newsfeed.internetmci.com!usenet.eel.ufl.edu!news.alt.net!news1.alt.net!news.u.washington.edu!uw-beaver!cornellcs!newsstand.cit.cornell.edu!news.graphics.cornell.edu!dsi.unimi.it!univ-lyon1.fr!jussieu.fr!univ-lille1.fr!ciril.fr!usenet From: "Luc Ferrari" Newsgroups: bionet.diagnostics Subject: Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: Fri, 8 Dec 95 09:42:42 +0100 Organization: CIRIL, Nancy, France Lines: 25 Message-ID: <48495.ferrari@ctrmed.u-nancy.fr> Reply-To: NNTP-Posting-Host: microferrari.ctrmed.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: 8859-1 To: gwade@physiology.uwo.ca gwade@physiology.uwo.ca wrote: >>> I just found a great new product from Kalyx Biosciences. AMPLISCRIPT >>> allows you to detect your PCR products using an ELISA format. >No labelled primers. >Works on 96 welled plates. >So now you have many copies of RNA which is complementary to the DNA >you were probing for. > >Kalyx has some literature outlining this process. > >Interestingly enough they also have an inexpensive matrix on which you >can do a nucleic acid hybridization in about 90 min (at room temp). > Hi, The process seems interesting, but can it be used for the detection of small PCR fragments, 100 to 300 pb, used to detect mRNA ? And can it be used to quantified finally the mRNA ? Luc Luc Ferrari Centre du Medicament - URA CNRS 597 Faculte de Pharmacie - Universite Henri Poincare Nancy, France From owner-diagnostics@net.bio.net Sat Dec 09 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!harbinger.cc.monash.edu.au!nntp.coast.net!lll-winken.llnl.gov!uwm.edu!newsfeed.internetmci.com!info.ucla.edu!agate!news.mindlink.net!line178.nwm.mindlink.net!INNOVATEK From: INNOVATEK@mindlink.bc.ca (INNOVATEK MEDICAL INC) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: Sat, 9 Dec 1995 23:16:01 GMT Organization: MIND LINK! - British Columbia, Canada Lines: 29 Message-ID: References: <30C89947.B37@cmainc.com> NNTP-Posting-Host: line178.nwm.mindlink.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] In article <30C89947.B37@cmainc.com> Jim Dougherty writes: >From: Jim Dougherty >Subject: Saliva Rapid HIV Test Announced >Date: Fri, 08 Dec 1995 15:00:07 -0500 >http://www.cmainc.com/hiv.htm WorldWide Medical Testing has announced >the worldwide availability of the Saliva Rapid HIV Test. Approved by the >USFDA for export-only, these test kits have been shown to be as >effective as the serum-based ELISA and Western blot tests. (Sensitivity >- 100%, Specificity - 98.07%). Non-invasive procedure is ideal in any >area where rapid (5-10 minutes), reliable results are required and >expensive lab and personnel are not available: developing nations, armed >forces, emergency medicine, disaster relief, etc. Additional information >is available at our Web site for government entities, health >organizations, exporters/importers, business developers, media, etc. >E-Mail requests to mailto:jimd@caminc.com >-- >WorldWide Medical Testing, Inc >Helping the World fight AIDS - Saliva Rapid HIV Test >http://www.cmainc.com/hiv.htm - jimd@cmainc.com Your statement about US FDA approval for export only is misleading and I consider it as false advertising. US FDA either approve or not approve a product period. There is no such thing as *approved for export only*. Your claim here is considered as *mislabelling* by US FDA. From owner-diagnostics@net.bio.net Sat Dec 09 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!news.flinet.com!usenet From: Jim Dougherty Newsgroups: bionet.diagnostics Subject: Saliva Rapid HIV Test Announced Date: Fri, 08 Dec 1995 15:00:07 -0500 Organization: WorldWide Medical Testing, Inc Lines: 15 Message-ID: <30C89947.B37@cmainc.com> NNTP-Posting-Host: 205.217.117.250 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (Win95; I) http://www.cmainc.com/hiv.htm WorldWide Medical Testing has announced the worldwide availability of the Saliva Rapid HIV Test. Approved by the USFDA for export-only, these test kits have been shown to be as effective as the serum-based ELISA and Western blot tests. (Sensitivity - 100%, Specificity - 98.07%). Non-invasive procedure is ideal in any area where rapid (5-10 minutes), reliable results are required and expensive lab and personnel are not available: developing nations, armed forces, emergency medicine, disaster relief, etc. Additional information is available at our Web site for government entities, health organizations, exporters/importers, business developers, media, etc. E-Mail requests to mailto:jimd@caminc.com -- WorldWide Medical Testing, Inc Helping the World fight AIDS - Saliva Rapid HIV Test http://www.cmainc.com/hiv.htm - jimd@cmainc.com From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!nott!cunews!freenet.carleton.ca!FreeNet.Carleton.CA!by207 From: by207@FreeNet.Carleton.CA (Satoko Hayashi) Subject: Fuscoporia obliqua Message-ID: Sender: by207@freenet.carleton.ca (Satoko Hayashi) Reply-To: by207@FreeNet.Carleton.CA (Satoko Hayashi) Organization: The National Capital FreeNet Date: Mon, 11 Dec 1995 21:33:05 GMT Lines: 17 I am looking for somebody who is involved in a research on Fuscoporia obliqua, a kind of mushrooms grown on white birch. Professor Ueno of Science University of Tokyo is seeking a collaborative project on AIDS treatment. I would appreciate any kind of information on this subject. Thank you. Satoko Hayashi by207@FreeNet.Carleton.CA From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: anj9000404@aol.com (ANJ9000404) Newsgroups: bionet.diagnostics Subject: Re: VZV, HSV Monoclonals Date: 11 Dec 1995 16:51:13 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 2 Sender: root@newsbf02.news.aol.com Message-ID: <4ai94h$6up@newsbf02.news.aol.com> References: <4afe4k$oe@newsbf02.news.aol.com> Reply-To: anj9000404@aol.com (ANJ9000404) NNTP-Posting-Host: newsbf02.mail.aol.com We were successful in detecting herpes zoster in fixed tissues using mix monoclonal antibodies from Viro Research (Phone: 800-230-4364). From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!djones From: djones@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Ampliscript Date: 11 Dec 1995 03:35:07 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512111132.LAA09763@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net For those of you who are interested I got some info through on Ampliscript today from Kalyx via there UK distributor. Which is:- Bionostics LTD The Buisness Centre Tythe farm Wyboston Beds. MK44 3AT. From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!gatech2!EU.net!peer-news.britain.eu.net!demon!user From: filter@firthcom.demon.co.uk (Steve Firth) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: Mon, 11 Dec 1995 11:15:12 +0000 Organization: Firth Consulting Lines: 21 Message-ID: References: <30C89947.B37@cmainc.com> <4afuvn$8e7@newsbf02.news.aol.com> NNTP-Posting-Host: firthcom.demon.co.uk X-NNTP-Posting-Host: firthcom.demon.co.uk In article <4afuvn$8e7@newsbf02.news.aol.com>, rscreager@aol.com (RSCreager) wrote: >An oral test for HIV with a sensitivity of 100%??? Compared to what? This advert seems to a rather slow spam. I saw it a couple of weeks ago in uk.misc and responded by email to the author. He has (suprisingly) never responded to my enquiry. I believe that it constitutes an offence to offer such a kit for sale within the EU, all determinations of a patients immune status to HIV infection must be accompanied by counselling to explain the test results and their significance. I also believe that the specificity of the kit indicates a tendency to produce false positive results. Perhaps this explains why the sensitivity is quoted as 100%? As you indicate, without further information the values quoted are meaningless and I suspect that this product is yet another scam. From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!rug.ac.be!mario.vaneechoutte From: mario.vaneechoutte@rug.ac.be Newsgroups: bionet.diagnostics Subject: (Fwd) AMPLISCRIPT - No gells, Quantitative, Double Fidelity Date: 10 Dec 1995 23:54:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512110751.AA09093@allserv.rug.ac.be> NNTP-Posting-Host: net.bio.net For your information: This was the message I received from Kalyx today. ------- Forwarded Message Follows ------- Date: Fri, 8 Dec 1995 18:51:10 -0500 To: Mario.Vaneechoutte@rug.ac.be From: kalyx@magi.com Subject: AMPLISCRIPT - No gells, Quantitative, Double Fidelity Dear Dr Vaneechoutte, Further to your message to the user group. I must apologise that you have not received the information, we sent it via air mail on 30th November. I am sorry we did not E mail it but at that time we did not have the material in electronic format. For your information we are in the final stages of developing a home page (http://www.io.org/~kalyx) . As yet we do not have an agent in Belgium - any suggestions ? Again please accept our apologies for the delay. Please do not hesitate to contact me if you require any addition information. David Smith VP Marketing David Smith Kalyx Biosciences Inc 20 Camelot Drive Nepean Ontario Canada K2G 5X8 Tel: (613) 723-1114 Fax: (613) 723-8777 Sincerely yours Mario Vaneechoutte Department Clinical Chemistry, Microbiology & Immunology University Hospital Blok A, De Pintelaan 185 B9000 Ghent Belgium Tel. +32 9 240 36 92 Fax. +32 9 240 36 59 E-mail: Mario.Vaneechoutte@rug.ac.be From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: biorecruit@aol.com (BioRecruit) Newsgroups: bionet.diagnostics Subject: POSITIONS Date: 10 Dec 1995 22:08:42 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: root@newsbf02.news.aol.com Message-ID: <4ag7bq$chf@newsbf02.news.aol.com> Reply-To: biorecruit@aol.com (BioRecruit) NNTP-Posting-Host: newsbf02.mail.aol.com Positions to open from Senior PhD Group Leader to technicians. A small growing Biotech Company specializing in In Vitro Diagnostic Kits and Reagents for the detection of virus antigens will be looking for top candidates. The company currently offers over 30 products one of which has been approved by the FDA to market. Products include human herpes virus-specific monoclonal and polyclonal antibodies, antigens, viral cell lysates, primers, probes and target DNA. For more information send to fax (815) 395-1473. From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rscreager@aol.com (RSCreager) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: 10 Dec 1995 19:45:43 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 10 Sender: root@newsbf02.news.aol.com Message-ID: <4afuvn$8e7@newsbf02.news.aol.com> References: <30C89947.B37@cmainc.com> Reply-To: rscreager@aol.com (RSCreager) NNTP-Posting-Host: newsbf02.mail.aol.com An oral test for HIV with a sensitivity of 100%??? Compared to what? All of the data I've seen to date indicate oral fluid testing for HIV 1 and/or HIV 1+2 antibodies are less than 100% when compared to serum collected from the same patient. Are these data published? What collection device did you use? How did you confirm reactives as their is no FDA approved saliva test for confirmation? Is your test specific for all known variants of HIV including tupe O? How many specimens were tested in your clinical trials, how many sites, how many lots of reagents, etc. Please let me know where I can obtain additonal information. Richard S. Creager From owner-diagnostics@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: phdhope@aol.com (PhD hope) Newsgroups: bionet.diagnostics Subject: VZV, HSV Monoclonals Date: 10 Dec 1995 14:58:12 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <4afe4k$oe@newsbf02.news.aol.com> Reply-To: phdhope@aol.com (PhD hope) NNTP-Posting-Host: newsbf02.mail.aol.com I am having no success with polyclonals for fixed tissue identification of herpes or zoster infections. Does anyone know of a source of monoclonals that may do the job for me? From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Norway.EU.net!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!nac.no!ifi.uio.no!news.sics.se!newsfeed.sunet.se!news01.sunet.se!sunic!news99.sunet.se!news.funet.fi!mordred.cc.jyu.fi!news From: Matti Vuento Newsgroups: bionet.diagnostics Subject: Chlamydial peptidase Date: Tue, 12 Dec 1995 11:35:26 -0800 Organization: University of Jyvaskyla, Finland Lines: 8 Message-ID: <30CDD97E.493B@dodo.jyu.fi> NNTP-Posting-Host: vap210a.pcbio.jyu.fi Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b1 (Windows; I; 16bit) I have heard claims that Chlamydia trachomatis has a specific peptidase ("peptidase 123" which can be used in chlamydial secreening. However, I have found no information supporting these claims in the Medline database. Does anyone know about this? Please send me E-mail if you do. Matti Vuento From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!veths.no!vigfrid.ness From: vigfrid.ness@veths.no (Vigfrid Ness) Newsgroups: bionet.diagnostics Subject: Video/printers Date: 12 Dec 1995 01:50:50 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512120950.BAA07155@net.bio.net> NNTP-Posting-Host: net.bio.net Hi! Have any one of you any good brand name for video chameras and black/white thermoprinters to use for the recording of gels etc.???? Vigfrid Ness Vigfrid Ness DYNAL A.S. Norway E.mail: vigfrid.ness@veths.no From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!rug.ac.be!mario.vaneechoutte From: mario.vaneechoutte@rug.ac.be Newsgroups: bionet.diagnostics Subject: Search for CviJII supplier Date: 12 Dec 1995 01:49:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512120947.AA07910@allserv.rug.ac.be> NNTP-Posting-Host: net.bio.net Hi, I am looking for a restriction enzyme with the designation CviJII (recognition sequence: RG/CY) which cuts very frequently and which could be useful for the work I am doing: restriction analysis of amplified genes. Although this enzyme is present in the restriction enzyme lists of DNA-software programs like DNAsis and PC/Gene, none of the companies I know seems to sell it. Can anyone give me a hint? Sincerely yours Mario Vaneechoutte Department Clinical Chemistry, Microbiology & Immunology University Hospital Blok A, De Pintelaan 185 B9000 Ghent Belgium Tel. +32 9 240 36 92 Fax. +32 9 240 36 59 E-mail: Mario.Vaneechoutte@rug.ac.be From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!PATH.ORG!mtam From: mtam@PATH.ORG (Milton Tam) Newsgroups: bionet.diagnostics Subject: Saliva HIV kit from WorldWide Medical Testing Date: 12 Dec 1995 10:45:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512121820.KAA26447@path.org> NNTP-Posting-Host: net.bio.net >X-POP3-Rcpt: mtam@path >Return-Path: INNOVATEK@mindlink.bc.ca >X-Sender: INNOVATEK@pop.mindlink.net >Date: Tue, 12 Dec 1995 01:41:45 -0800 >To: mtam@path.org >From: INNOVATEK@mindlink.bc.ca (INNOVATEK MEDICAL INC) >Subject: Saliva HIV kit from WorldWide Medical Testing > >Thank you for the e-mail. I suggest you report this misleading advertising >to US FDA. It appears WorldWide Medical Testing is located in USA. > >There are laws in USA and within the power of FDA to take action agaist this >kind of claim. > >Regards, > >Mr. King F. Hui >Vancouver, Canada. > > ****************************************************************** Milton R. Tam Program for Appropriate Technology in Health (PATH) 4 Nickerson Street Seattle, WA 98109 phone (206) 285-3500, fax (206) 285-6619 ****************************************************************** From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!psinntp!psinntp!psinntp!psinntp!news.netvision.net.il!usenet From: gslutzky@netvision.net.il Newsgroups: sci.med.immunology,bionet.immunology,bionet.diagnostics Subject: Autoimmune antigens and sera Date: Tue, 12 Dec 95 14:02:30 PDT Organization: NetVision LTD. Lines: 7 Message-ID: NNTP-Posting-Host: ts2ap15.netvision.net.il Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Xref: biosci sci.med.immunology:3513 bionet.immunology:6599 bionet.diagnostics:472 We are looking for suppliers of immunopurified autoimmune antigens (SS-A, SS-B, Sm, RNP, etc.) as well as for quantities of well defined patients' sera from individuals with autoimmune diseases. Please send information or catalogs to gslutzky@netvision.net.il or fax to 972-2-781852. From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk Newsgroups: bionet.diagnostics Subject: Re: ---Please Read This--- Date: 12 Dec 1995 05:49:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512121345.aa24857@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net > Who is editing the submissions to this group? Who let this jerk on who is > advertising for a pyramid scheme? Read carefully. This type of stuff is > illegal. > Like virtually all bionet groups, this one is unmoderated. I understand your annoyance with this kind of post, sadly as the internet expands we are going to be getting more of it. The best thing to do is to ignore it or to complain to the postmaster at the originating site. See the recently posted mini-faq from Dave Kristofferson, for more information on how to deal with spam. Kevin O'Donnell SASA Edinburgh Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.euro.net!usenet From: bioup@euronet.nl (Andre W. Schram) Newsgroups: bionet.diagnostics Subject: ANNOUNCEMENT: Courses in Protein and Carbohydrate (Bio)technology Date: 12 Dec 1995 19:33:20 GMT Organization: BioUpdate Foundation Lines: 32 Sender: -Not-Authenticated-[8712] Message-ID: <4akle0$39m@news.euro.net> NNTP-Posting-Host: p011.mas.euronet.nl X-Posted-From: InterNews 1.0.6@p011.mas.euronet.nl Xdisclaimer: No attempt was made to authenticate the sender's name. RESIDENTIAL POST-EXPERIENCE EDUCATION AND TRAINING FOR THE BIOTECHNOLOGY AND ALLIED INDUSTRIES. Established in the Netherlands in 1992, the BioUpdate Foundation promotes residential post-experience education and training for the biotechnology and allied industries. Courses are designed primarily for industrial scientists who are actively involved in protein and carbohydrate (Bio)technology. Courses will also cater for technologists and engineers charged with the development of new products/processes in which proteins and carbohydrates form key elements. Finally, it will help academic and medical researchers and postgraduate students to become familiar with the issues and problems which face those involved in the fast growing "protein and carbohydrate industry". The BioUpdate Foundation also organises workshops and scientific meetings on a selected number of topics related to the areas of protein and carbohydrate (bio)technology. First course to come: "Modules in Biotechnology", to be held March 1996 in the Netherlands. ------------------------------------------------------------------------ ----- For details on events, please visit the BioUpdate Homepage at: http://www.euronet.nl/users/bioup or e-mail to: bioup@euronet.nl From owner-diagnostics@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!mail2news.demon.co.uk!scottp.demon.co.uk From: Richard Scott Newsgroups: bionet.diagnostics Subject: EPIDEMIOLOGICAL SURVEY IN RURAL ASIA? Date: Wed, 13 Dec 1995 00:17:31 GMT Organization: Myorganisation Lines: 25 Message-ID: <677256757wnr@scottp.demon.co.uk> Reply-To: Richard@scottp.demon.co.uk X-NNTP-Posting-Host: scottp.demon.co.uk X-Broken-Date: Wednesday, Dec 13, 1995 00.17.31 X-Newsreader: Newswin Alpha 0.7 X-SMTP-Posting-Host: scottp.demon.co.uk [Wed, 13 Dec 95 0:25:39 GMT] X-SMTP-Posting-Host: post.demon.co.uk [Wed, 13 Dec 95 0:26:20 GMT] I am a 2nd year pre-clinical medical student at Cambridge University (UK); A friend (another medic from Cambridge), are travelling in Tibet for 3 months next summer (July until October) and are interested in carrying out a medically related survey. We will be travelling in 'urban' areas, but probably of more use would be the contact we will also have with very rural (including nomadic) communities. We anticipate that we will trek in such rural (high altitude) areas for at least a month of the 3. We would be more than happy to carry out a survey as part of someone elses research. Possibilities might include surveys into: (1) immunization programs (2) disease epidemiology (3) genetic disease prevalence (4) water quality (especially water borne diseases) (5) nutrition however, we are open to your suggestions. Thank you very much, Richard Scott e-mail: richard@scottp.demon.co.uk From owner-diagnostics@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!oleane!jussieu.fr!univ-lyon1.fr!rockendo.univ-lyon1.fr!pcomm From: pcomm@cismibm.univ-lyon1.fr (Pierre Commercon) Newsgroups: bionet.diagnostics Subject: Diagnostic Kit in Molecular Biology Date: Wed, 13 Dec 1995 15:25:17 Organization: Universite Lyon1 Pharmacie Lines: 15 Message-ID: NNTP-Posting-Host: rockendo.univ-lyon1.fr X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hi I am looking for a list of the different Diagnostic kit in molecular biology on biochimy, bacteriology, virology, genetic..... It is for a laboratory (not a research lab.), so they want buy ready to use kits. Merci Pierre Pcomm@cismibm.univ-lyon1.fr From owner-diagnostics@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!UNLINFO.UNL.EDU!rfrench From: rfrench@UNLINFO.UNL.EDU (roy french) Newsgroups: bionet.diagnostics Subject: Re: CviJI Date: 13 Dec 1995 08:25:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512131623.AA23366@unlinfo.unl.edu> NNTP-Posting-Host: net.bio.net Hello Mario, The restriction nuclease you are after with recognition sequence: RG/CY is CviJI, which is produced by a large DNA virus of a eukaryotic alga (Chlorella sp.). This and related viruses encode a number of restriction and modification systems, some with unique recognition sequences. These enzymes were originally discovered here at UNL in Jim Van Etten's lab. They are commercially available from the following company: Megabase Research Products 2820 N. 48th Street, Suite 110 Lincoln, NE Phone: (402) 467-6499 Fax: (402) 472-2853 As you point out, frequent cutters like CviJI are ideal for analysis of short PCR products--I've used it to distinguish strains of a plant virus. Best Wishes, Roy French, USDA, ARS From owner-diagnostics@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!COLUMBIA.EDU!jjk5 From: jjk5@COLUMBIA.EDU (John Krolewski) Newsgroups: bionet.diagnostics Subject: Automated/simple DNA extraction Date: 13 Dec 1995 07:40:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512131538.AA23240@mailhub.cc.columbia.edu> NNTP-Posting-Host: net.bio.net I am looking for ways to speed extraction of DNA for use in Southern blotting and PCR based diagnostic tests. 1. Can anybody provide info/critique on automated DNA extraction equipment (high molecular weight)? How do these compare, w.r.t. time, yield and DNA quality, against reagents like DNAzol? 2. Can anybody provide info/critique on matrices such as S&S IsoCode for simple isolation of DNA from blood (low molecular weight suitable for PCR). From owner-diagnostics@net.bio.net Wed Dec 13 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news1.cris.com!news From: wick@cris.com Newsgroups: bionet.diagnostics Subject: RE: CviJI Date: 14 Dec 1995 19:53:48 GMT Organization: Molecular Biology Resources, Inc. Lines: 15 Message-ID: <4apvcc$do3@spectator.cris.com> NNTP-Posting-Host: crc4-fddi.cris.com X-Newsreader: AIR News 3.X (SPRY, Inc.) CviJI is carried by CHIMERx Phone: 800-626-7833 Jim Wick E-mail: wick@cris.com Phone: 414-871-7199 FAX: 414-871-1273 Snail Mail: 5520 W. Burleigh St. Milwaukee, WI 53210 "Don't precipitate more than you can resuspend" From owner-diagnostics@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!EU.net!sun4nl!xs4all!usenet From: biopharm@xs4all.nl (dr. ing. Harold Stringer) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: Fri, 15 Dec 1995 13:02:32 GMT Organization: Ridascreen - Biopharm BV Lines: 20 Message-ID: <4arqtc$1iu@news.xs4all.nl> References: <30C89947.B37@cmainc.com> <4afuvn$8e7@newsbf02.news.aol.com> Reply-To: biopharm@xs4all.nl NNTP-Posting-Host: asd01-03.dial.xs4all.nl X-Newsreader: Forte Free Agent 1.0.82 filter@firthcom.demon.co.uk (Steve Firth) wrote: >I believe that it constitutes an offence to offer such a kit for sale >within the EU, all determinations of a patients immune status to HIV >infection must be accompanied by counselling to explain the test results >and their significance. As far as I know, counselling is not obligatory in the Netherlands, but strongly advised. >I also believe that the specificity of the kit >indicates a tendency to produce false positive results. Perhaps this >explains why the sensitivity is quoted as 100%? >As you indicate, without further information the values quoted are >meaningless and I suspect that this product is yet another scam. I agree, further information is essential. Harold Stringer From owner-diagnostics@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.kei.com!nntp.coast.net!col.hp.com!news.dtc.hp.com!hpscit.sc.hp.com!news.an.hp.com!news From: David Sherrill Newsgroups: bionet.diagnostics,sci.med.diseases.cancer,sci.med.physics,sci.techniques.mag-resonance Subject: Re: Electromagnetic Fields and Health Date: Fri, 15 Dec 1995 10:02:01 -0500 Organization: Hewlett Packard Andover Site Lines: 26 Message-ID: <30D18DE9.51F@an.hp.com> References: <4aqj8i$3mc$17@mhafc.production.compuserve.com> NNTP-Posting-Host: hpangrmc.an.hp.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (X11; I; HP-UX A.09.05 9000/735) Xref: biosci bionet.diagnostics:484 sci.med.diseases.cancer:6752 sci.med.physics:3885 sci.techniques.mag-resonance:1394 Maren Oelbermann wrote: > The issue of electromagnetic fields and human health is a > controversial scientific, and often public, issue. A new book... The controversy is most endemic among those who stand to gain from continuing this farce. The PBS program NOVA aired a fair and balanced story on this subject a month or two ago. Read any books you like, but make sure you understand the "Texas Marksman" and other statistical frauds first. See also http://www.calpoly.edu/~dhafemei/apspowerline.html for the Statement by the Council of the American Physical Society, April 22, 1995, "POWER LINE FIELDS AND PUBLIC HEALTH". IMHO, the real risk to the public from this subject is the squandering of rare and valuable medical research funds on a subject that at one time may have been a legitimate concern but now has been put to rest by careful investigation. Even if one is not conviced that there is zero risk here, one must agree that there are much more pressing health problems better deserving of research investment. David Sherrill [Opinion's are my own, not my employer's... but you knew that!] From owner-diagnostics@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Maren Oelbermann <103263.2013@CompuServe.COM> Newsgroups: bionet.diagnostics Subject: Electromagnetic Fields and Health Date: 15 Dec 1995 01:19:31 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 130 Message-ID: <4aqif3$3mc$3@mhafc.production.compuserve.com> The issue of electromagnetic fields and human health is a controversial scientific, and often public, issue. A new book "takes a comprehensive look at the growing EMF controversy, and explains clearly for non-technical readers the basics of EMF research." It is fully referenced and people from health professionals to homeowners will find it useful. It explains what EMFs are and where they are found. It discusses the basic findings and exciting new research related to bioelectromagnetics; it addresses public dimensions including risk assessment and mitigation; and it ends with a chapter on prudent avoidance, helping people understand what they can do to simply and inexpensively reduce exposure. The authors -- biophysicist Michael Milburn and biologist Maren Oelbermann -- have been involved with environmental EMFs since 1990, working for ELMAG Research and Consulting in Waterloo, On, Canada. Their work on EMF's has been profiled in the media, and through public speaking and talk shows. Here are some excerpts from ELECTROMAGNETIC FIELDS AND YOUR HEALTH, by Michael Milburn and Maren Oelbermann, published by New Star Books, Vancouver, BC From Chapter on BASIC SCIENCE There is a real sense of excitement and drama surrounding this whole process. Those scientists studying cells are making observations that are not, according to conventional thinking, supposed to occur. Faced with limited funding and often a lack of support from their peers, these scientists are none the less pushing ahead to explore what they describe as one of the scientific frontiers of this century. The search to observe previously unknown phenomenon is also joined with the job of explaining how these phenomenon come about. New medical breakthroughs and a deeper understanding of the way in which living things work are goals of this flurry of activity. The excitement of this scientific frontier is perhaps overshadowed by the drama inherent in electromagnetic field epidemiology. As a result of the far reaching consequences of the outcome of the epidemiological research, the results of each study become headline news and with public involvement and interest assured, the controversy of this new subject is heightened. In Vitro Studies Scientists hope that by studying simpler cellular systems, the basic principles of the interaction between weak energy fields and living things can be understood. One major limitation of in vitro experimentation however, is that the results and conclusions of studies carried out on cells in a test tube cannot easily be used to predict how humans or animals will respond to electromagnetic energy fields. This is due to the complexity of physiological systems composed of groups of cells which interact to make up a complete organism. In vitro experiments represent only one level of the overall effort to learn more about how weak electromagnetic energy fields can affect living things; further scientific research is still required to answer questions about human interactions with weak fields. Examples of In Vitro Research Our immune system helps to defend against viruses and bacteria and its role in preventing the excessive growth of cancerous cells continues to be investigated. The health of the immune system is intimately connected to the proper functioning of the whole body. It is of interest then, to determine all the factors which might influence the immune system. Weak electromagnetic energy fields are now being studied in this context. In vitro experiments with cellular components of the immune system have been carried out by D.B. Lyle and his co-workers.9 These researchers found a 25 percent reduction in the activity of T-lymphocytes that were exposed to 60 Hz electric fields. The T-lymphocytes are white blood cells that helps to destroy antigens as part of an immune response. A reduced competency of the immune system due to electromagnetic energy fields may be related to the question of cancer. Other researchers have also chosen T-lymphocytes to study immune system electromagnetic field effects.10 Calcium plays an important role in acting as a signal between the exterior and the interior of the cell and interference with the calcium signal has been shown to cause significant changes in cell behaviour. These researchers examined whether electromagnetic fields could influence the cells immune response by producing changes in the calcium signal. They felt that if changes in calcium signalling could be observed, important features of the immune system may be influenced by electromagnetic fields. The researchers' results were quite interesting. If the T-lymphocytes used in these studies did not have their calcium signalling stimulated, then the cells showed no response to weak fields. However, when the cells were activated by an immune stimulating molecule, there was a response to electromagnetic fields. In one experiment, for example, the researchers observed a 2.4 fold increase in calcium transport after cells were immune-stimulated. If these same cells were exposed to 60 Hz fields, a 3.9-fold increase in calcium transport occurred after immune- stimulation of the cell. These results demonstrate once again that fields, theoretically too weak to be biologically important, can affect fundamental processes of living organisms. One of the scientists involved in this research, Dr. Jan Walleczek, suggests that weak electromagnetic fields may thus be able to influence human and animal immune responses. He mentions that six laboratories have in fact, observed immune system changes in animals as a result of field exposure. From Chapter on PUBLIC DIMENSIONS A specific example from electromagnetic field risks will help to demonstrate these first stages. The Feychting-Ahlbom study of power line fields and caner (see Chapter 3) showed a link between magnetic fields and childhood leukaemia. It was a powerful study because it found a dose-response relationship -- exposure to stronger magnetic fields meant higher risks. A dose- response relationship means an association between magnetic fields and childhood cancer can be more confidently made, and the risks form different fields can be more precisely determined. The Feychting-Ahlbom study prompted Jack Nou the Director of Electrical Safety for the Swedish National Board for Industrial and Technological Development to comment that a field- cancer link is 80 per cent certain and that his board must take action now and not wait until the 100 per cent level is achieved.1 Nou was quoted in the New Scientist saying that because of the Feychting-Ahlbom study,m he will proceed on the assumption that there is a link between power line magnetic fields and childhood leukaemia. In the first stage of the risk process, Nou noted that in the Swedish population as a whole, 3.5 new cases childhood leukaemia could be expected each year and as an example of the second stage, that he will make a careful study of the costs of reducing exposures in a report to the government. There is an obvious need for public involvement in risk management decisions and fortunately there is a movement in risk management towards this end. Before we explore examples of how the electromagnetic fields and health issue has entered the public domain, we can highlight the need for public involvement in risk management decisions by quoting Dr. M. Granger Morgan, head of the Department of Engineering and Public Policy at Carnegie Mellon University, and a researcher long involved in risk analysis and management. Morgan represents this positive and long overdue trend for a recognition of the importance of public involvement in deciding how we `manage' the technologies around us. "My experience and that of my colleagues indicate that the public can be very sensible about risk when companies, regulators and other institutions give it the opportunity. Laypeople have different, broader definitions of risk, which in important respects can be more rational than the narrow ones used by experts. Furthermore, risk management is, fundamentally, a question of values. In a democratic society, there is no acceptable way to make these choices without involving the citizens who will be affected by them.2 Legal Issues "Utilities across the country are feeling the heat. Since the mid 1980's, power companies have been involved in more than 100 lawsuits involving possible health hazards from transmission lines, especially those near schools. The patchwork of lawsuits, public hearings and local government rulings is having a direct impact."11 -- from Transmission and Distribution, a trade magazine for electrical Utility Managers ELECTROMAGNETIC FIELDS AND YOUR HEALTH, by Michael Milburn and Maren Oelbermann (NEW STAR BOOKS, Vancouver, BC) is available at better bookstores across the US and Canada or can be ordered directly from the authors for $15.00 US ($12. + $3.00 S/H) or in Canada for $20.00 Canadian, including S/H and GST: ELMAG Research and Consulting Suite 11-421 Barrie Place Waterloo, On Canada N2L 3Z6 The publisher can be contacted at: New Star Books Voice (604) 738-9429 Fax: (604) 738-9332 From owner-diagnostics@net.bio.net Thu Dec 14 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Maren Oelbermann <103263.2013@CompuServe.COM> Newsgroups: bionet.diagnostics,sci.med.diseases.cancer,sci.med.physics,sci.techniques.mag-resonance Subject: Electromagnetic Fields and Health Date: 15 Dec 1995 01:33:06 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 130 Message-ID: <4aqj8i$3mc$17@mhafc.production.compuserve.com> Xref: biosci bionet.diagnostics:481 sci.med.diseases.cancer:6729 sci.med.physics:3883 sci.techniques.mag-resonance:1388 The issue of electromagnetic fields and human health is a controversial scientific, and often public, issue. A new book "takes a comprehensive look at the growing EMF controversy, and explains clearly for non-technical readers the basics of EMF research." It is fully referenced and people from health professionals to homeowners will find it useful. It explains what EMFs are and where they are found. It discusses the basic findings and exciting new research related to bioelectromagnetics; it addresses public dimensions including risk assessment and mitigation; and it ends with a chapter on prudent avoidance, helping people understand what they can do to simply and inexpensively reduce exposure. The authors -- biophysicist Michael Milburn and biologist Maren Oelbermann -- have been involved with environmental EMFs since 1990, working for ELMAG Research and Consulting in Waterloo, On, Canada. Their work on EMF's has been profiled in the media, and through public speaking and talk shows. Here are some excerpts from ELECTROMAGNETIC FIELDS AND YOUR HEALTH, by Michael Milburn and Maren Oelbermann, published by New Star Books, Vancouver, BC From Chapter on BASIC SCIENCE There is a real sense of excitement and drama surrounding this whole process. Those scientists studying cells are making observations that are not, according to conventional thinking, supposed to occur. Faced with limited funding and often a lack of support from their peers, these scientists are none the less pushing ahead to explore what they describe as one of the scientific frontiers of this century. The search to observe previously unknown phenomenon is also joined with the job of explaining how these phenomenon come about. New medical breakthroughs and a deeper understanding of the way in which living things work are goals of this flurry of activity. The excitement of this scientific frontier is perhaps overshadowed by the drama inherent in electromagnetic field epidemiology. As a result of the far reaching consequences of the outcome of the epidemiological research, the results of each study become headline news and with public involvement and interest assured, the controversy of this new subject is heightened. In Vitro Studies Scientists hope that by studying simpler cellular systems, the basic principles of the interaction between weak energy fields and living things can be understood. One major limitation of in vitro experimentation however, is that the results and conclusions of studies carried out on cells in a test tube cannot easily be used to predict how humans or animals will respond to electromagnetic energy fields. This is due to the complexity of physiological systems composed of groups of cells which interact to make up a complete organism. In vitro experiments represent only one level of the overall effort to learn more about how weak electromagnetic energy fields can affect living things; further scientific research is still required to answer questions about human interactions with weak fields. Examples of In Vitro Research Our immune system helps to defend against viruses and bacteria and its role in preventing the excessive growth of cancerous cells continues to be investigated. The health of the immune system is intimately connected to the proper functioning of the whole body. It is of interest then, to determine all the factors which might influence the immune system. Weak electromagnetic energy fields are now being studied in this context. In vitro experiments with cellular components of the immune system have been carried out by D.B. Lyle and his co-workers.9 These researchers found a 25 percent reduction in the activity of T-lymphocytes that were exposed to 60 Hz electric fields. The T-lymphocytes are white blood cells that helps to destroy antigens as part of an immune response. A reduced competency of the immune system due to electromagnetic energy fields may be related to the question of cancer. Other researchers have also chosen T-lymphocytes to study immune system electromagnetic field effects.10 Calcium plays an important role in acting as a signal between the exterior and the interior of the cell and interference with the calcium signal has been shown to cause significant changes in cell behaviour. These researchers examined whether electromagnetic fields could influence the cells immune response by producing changes in the calcium signal. They felt that if changes in calcium signalling could be observed, important features of the immune system may be influenced by electromagnetic fields. The researchers' results were quite interesting. If the T-lymphocytes used in these studies did not have their calcium signalling stimulated, then the cells showed no response to weak fields. However, when the cells were activated by an immune stimulating molecule, there was a response to electromagnetic fields. In one experiment, for example, the researchers observed a 2.4 fold increase in calcium transport after cells were immune-stimulated. If these same cells were exposed to 60 Hz fields, a 3.9-fold increase in calcium transport occurred after immune- stimulation of the cell. These results demonstrate once again that fields, theoretically too weak to be biologically important, can affect fundamental processes of living organisms. One of the scientists involved in this research, Dr. Jan Walleczek, suggests that weak electromagnetic fields may thus be able to influence human and animal immune responses. He mentions that six laboratories have in fact, observed immune system changes in animals as a result of field exposure. From Chapter on PUBLIC DIMENSIONS A specific example from electromagnetic field risks will help to demonstrate these first stages. The Feychting-Ahlbom study of power line fields and caner (see Chapter 3) showed a link between magnetic fields and childhood leukaemia. It was a powerful study because it found a dose-response relationship -- exposure to stronger magnetic fields meant higher risks. A dose- response relationship means an association between magnetic fields and childhood cancer can be more confidently made, and the risks form different fields can be more precisely determined. The Feychting-Ahlbom study prompted Jack Nou the Director of Electrical Safety for the Swedish National Board for Industrial and Technological Development to comment that a field- cancer link is 80 per cent certain and that his board must take action now and not wait until the 100 per cent level is achieved.1 Nou was quoted in the New Scientist saying that because of the Feychting-Ahlbom study,m he will proceed on the assumption that there is a link between power line magnetic fields and childhood leukaemia. In the first stage of the risk process, Nou noted that in the Swedish population as a whole, 3.5 new cases childhood leukaemia could be expected each year and as an example of the second stage, that he will make a careful study of the costs of reducing exposures in a report to the government. There is an obvious need for public involvement in risk management decisions and fortunately there is a movement in risk management towards this end. Before we explore examples of how the electromagnetic fields and health issue has entered the public domain, we can highlight the need for public involvement in risk management decisions by quoting Dr. M. Granger Morgan, head of the Department of Engineering and Public Policy at Carnegie Mellon University, and a researcher long involved in risk analysis and management. Morgan represents this positive and long overdue trend for a recognition of the importance of public involvement in deciding how we `manage' the technologies around us. "My experience and that of my colleagues indicate that the public can be very sensible about risk when companies, regulators and other institutions give it the opportunity. Laypeople have different, broader definitions of risk, which in important respects can be more rational than the narrow ones used by experts. Furthermore, risk management is, fundamentally, a question of values. In a democratic society, there is no acceptable way to make these choices without involving the citizens who will be affected by them.2 Legal Issues "Utilities across the country are feeling the heat. Since the mid 1980's, power companies have been involved in more than 100 lawsuits involving possible health hazards from transmission lines, especially those near schools. The patchwork of lawsuits, public hearings and local government rulings is having a direct impact."11 -- from Transmission and Distribution, a trade magazine for electrical Utility Managers ELECTROMAGNETIC FIELDS AND YOUR HEALTH, by Michael Milburn and Maren Oelbermann (NEW STAR BOOKS, Vancouver, BC) is available at better bookstores across the US and Canada or can be ordered directly from the authors for $15.00 US ($12. + $3.00 S/H) or in Canada for $20.00 Canadian, including S/H and GST: ELMAG Research and Consulting Suite 11-421 Barrie Place Waterloo, On Canada N2L 3Z6 The publisher can be contacted at: New Star Books Voice (604) 738-9429 Fax: (604) 738-9332 From owner-diagnostics@net.bio.net Fri Dec 15 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: Gianpaolo Grassi Newsgroups: bionet.diagnostics Subject: Multistepper tips Date: 16 Dec 1995 12:06:39 -0000 Lines: 35 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4aucof$81@mserv1.dl.ac.uk> X-Sender: ggrassi@genes Original-To: diagnost@dl.ac.uk This message is in MIME format. The first part should be readable text, while the remaining parts are likely unreadable without MIME-aware tools. Send mail to mime@docserver.cac.washington.edu for more info. --587202790-1739686943-819114717=:18216 Content-Type: TEXT/PLAIN; charset=US-ASCII Dear mailer, I use the Multistepper pipette from Labsystem and I am not satisfied of the original "new version" of tips. They are irregular in shape and very difficult tu use for tissue culture procedures. Please, let me know other companies that produce similar tips with better quality. Thanks, Giampaolo --587202790-1739686943-819114717=:18216 Content-Type: TEXT/PLAIN; charset=US-ASCII; name=isci Content-Transfer-Encoding: BASE64 Content-ID: Content-Description: KioqKioqKioqKioqKioqKioqKioqKioqKkUtTUFJTCoqKioqKioqKioqKioq KioqKioqKioqKioqKioNCiogICAgIEdSQVNTSSBHSUFNUEFPTE8gIFJlc2Vh cmNoIFBsYW50IFBoYXRob2xvZ2lzdCAgICAgICAqDQoqICAgICAgICAgICAg ICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAg Kg0KKiAgICBJc3RpdHV0byBTcGVyaW1lbnRhbGUgcGVyIGxlIENvbHR1cmUg SW5kdXN0cmlhbGkgICAgICoNCiogICAgVmlhIGRpIENvcnRpY2VsbGEgMTMz LCA0MDEyOSwgQm9sb2duYSwgSXRhbHksIElUICAgICAgKg0KKiAgICAgICAg ICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAgICAg ICAgICoNCiogICAgVGVsLiArMzktNTEtMzcxMTU3LCArMzktNTEtMzc0MjIx ICAgICAgICAgICAgICAgICAgICAqDQoqICAgIEZheC4gKzM5LTUxLTM3NDg1 NywgRS1NYWlsOiBnZ3Jhc3NpQGljZ2ViLnRyaWVzdGUuaXQgKg0KKiAgICAy IG5vZGU6IExLNUJPSTMxQGNpbmU4OC5jaW5lY2EuaXQgICAgICAgICAgICAg ICAgICAgICoNCioqKioqKioqKioqKioqKioqKioqKioqKioqKioqKioqKioq KioqKioqKioqKioqKioqKioqKioqKioqICAgIA0KICAgICAgICAgICAgICAN CiAgIA0K --587202790-1739686943-819114717=:18216-- From owner-diagnostics@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!DCOOKE From: DCOOKE@SCRI.SARI.AC.UK ("David Cooke") Newsgroups: bionet.diagnostics Subject: unsubscribe Date: 18 Dec 1995 01:00:58 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <225E17932A1@law.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net unsubscribe diagnost David E. L. Cooke Mycology and Bacteriology Dept. Scottish Crop Research Institute Invergowrie, Dundee, DD1 5DA. Tel 0382 562731 FAX 0382 562426 e-mail dcooke@scri.sari.ac.uk From owner-diagnostics@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!SCRI.SARI.AC.UK!DCOOKE From: DCOOKE@SCRI.SARI.AC.UK ("David Cooke") Newsgroups: bionet.diagnostics Subject: subscribe Date: 18 Dec 1995 01:01:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <225E6666DDF@law.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net subscribe diagnost change of user-id! David E. L. Cooke Mycology and Bacteriology Dept. Scottish Crop Research Institute Invergowrie, Dundee, DD1 5DA. Tel 0382 562731 FAX 0382 562426 e-mail dcooke@scri.sari.ac.uk From owner-diagnostics@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!gossip.pyramid.com!news.sedona.net!usenet From: Ann Rathbun Newsgroups: bionet.diagnostics Subject: Research Investigator, Microbiology Date: 18 Dec 1995 23:57:22 GMT Organization: Sedona Internet Services, Inc. Lines: 50 Message-ID: <4b4v52$rlf@labrat.sedona.net> NNTP-Posting-Host: client17.sedona.net Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Research Investigator, Microbiology We are seeking a Ph.D. scientist with a minimum of 2 years post doctorate to supervise complex development projects in formulation of infection control products. He/she will design and implement new concepts for testing and evaluation of these products, will supervise microbiology testing, improve efficiency of test methods, and will also work closely with Sales & Marketing, Regulatory and Clinical Affairs. This person must be a hands-on scientist with a strong bacterial background. Successful candidates will have exceptionally strong communication skills as he/she will work with Sales & Marketing to develop clearly written technical notices, and will work with Regulatory on FDA filings and with Clinical Affairs to assist in the design of appropriate clinical trials. Desired Profile: * Ph.D., Microbiology with 2 years post-doctoral experience or MS with strong bacterial background and 5+ years in a Microbiology function. * Strong research/clinical/industrial experience in bacteriology, such as identification of bacteria, sensitivity testing, methods development, challenge testing. * Enthusiastic scientist who would relish an opportunity to be an expert in skin microbiology. * Excellent presentation and communication skills and a person who enjoys working and works well with people from a variety of departmental perspectives. This is an opportunity to become an expert in skin microbiology, and to continue with scientific publication and presentations. The group has excellent visibility and the opportunity to make a significant impact of the company’s product line. Our client is a top pharmaceutical company with a strong portfolio; they will handle relocation and have an excellent benefit package. If you have an interest in this or other opportunities, please mail or FAX your CV/resume to RS&A to the attention of Ann G. Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 284-3360 Office (520)284-3361 FAX E-mail: rathbun@ sedona.net From owner-diagnostics@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!oleane!jussieu.fr!centre.univ-orleans.fr!univ-lyon1.fr!dsi.unimi.it!master.cci.unibs.it!lunardi From: lunardi@master.cci.unibs.it (ITCPACLE LUNARDI - BRESCIA) Newsgroups: bionet.diagnostics Subject: HELP: rare illness Date: Wed, 20 Dec 1995 10:29:18 Organization: Computer Science Dep. - Milan University Lines: 30 Message-ID: NNTP-Posting-Host: 192.167.18.170 X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Urgently Required Detailed information and the address of any hospitals or research institutes specialized in diseases of the blood especially anything relating to the diseases known as the MOSCHOWITZ SYNDROME OR THROMBOTIC MICRO ANGIOPATHY. Please send all information possible to any one of the following addresses: FAX:+39 30 6577098 TEL.: +39 30 657117 -657143 --------------------------------------------------------------------------------- MESSAGGIO URGENTISSIMO. Si richiedono dettagliate informazioni ed eventuali indirizzi di centri specializzati nello studio e nella cura di malattie del sangue: in particolare oggetto della richiesta č la malattia denominata PORPORA TROMBOTICA TROMBOCITOPENICA detta anche SINDROME DI MOSCHOWITZ, MICROANGIOPATICA TROMBOTICA. Le informazioni in nostro possesso sono: -malattia rara del bambino e dell’adulto, caratterizzata anatomicamente da una lesione diffusa delle arteriole e dei capillari che associa in spessimento dell’endotelio, deposito sotto-endoteliale di sostanze fibrinoidi e trombosi; clinicamente da una insorgenza acuta febbrile, una anemia emolitica intensa con emazie deformate, una porpora e a volte emorragie legate ad un abbassamento del tasso delle piastrine ematiche, lesioni renali con ematuria ed azotemia, manifestazioni neurologiche fugaci e variabili ed un evoluzione a poussčes e remissioni, ma sempre mortale. -rientra nel quadro delle anemie emolitiche microangiopatiche. DATA L’URGENZA Vi chiediamo inoltre di farci pervenire, se possibile, quanto richiesto ad uno dei seguenti recapiti: FAX: +39 30 6577098 TEL.: +39 30 657117 -657143 From owner-diagnostics@net.bio.net Tue Dec 19 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!noc.near.net!news3.near.net!yale!yale.edu!news.ycc.yale.edu!news From: Sandeep Laumas Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: 20 Dec 1995 21:43:51 GMT Organization: Yale University Lines: 20 Message-ID: <4ba02n$8lf@news.ycc.yale.edu> References: <30C89947.B37@cmainc.com> NNTP-Posting-Host: 130.132.29.28 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; PPC) To: INNOVATEK@mindlink.bc.ca X-URL: news:INNOVATEK.43.30CA18B1@mindlink.bc.ca INNOVATEK@mindlink.bc.ca (INNOVATEK MEDICAL INC) wrote: >In article <30C89947.B37@cmainc.com> Jim Dougherty >>Subject: Saliva Rapid HIV Test Announced >>Date: Fri, 08 Dec 1995 15:00:07 -0500 > WorldWide Medical Testing has announced >>the worldwide availability of the Saliva Rapid HIV Test. Approved by the >>USFDA for export-only, these test kits have been shown to be as >>effective as the serum-based ELISA and Western blot tests. (Sensitivity >>- 100%, Specificity - 98.07%). >>WorldWide Medical Testing, Inc >>Helping the World fight AIDS - Saliva Rapid HIV Test Dear Sir: I strongly suggest you check with the FDA in your MISLEADING advertising of using the FDA to gain approval. This is illegal, even though you think you may be in Canada!! From owner-diagnostics@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.uoregon.edu!waikato!RPC216 From: COLLINR@Agresearch.cri.nz (ROGER COLLIN) Newsgroups: bionet.diagnostics Subject: ELISA plate storage Date: Thu, 21 Dec 95 01:58:05 GMT Organization: AgResearch Ruakura Lines: 20 Message-ID: <4bab5d$17p0@thebes.waikato.ac.nz> NNTP-Posting-Host: 160.4.128.189 X-Newsreader: News Xpress Version 1.0 Beta #4 I have developed a direct competitive ELISA assasy which works very well. However I would like to be able to store my plates so that I can use them as required rather than having to coat and block them immediatly before use. Does anyone have any experience in storage of ELISA plates which have already been coated with antibody? and could whoever you are please contact me about the best means to go about doing this. I have made some attempts at storage which have involved drying the plate after blocking (I use PVP as a blocker) with and without a 5% sucrose rinse, which apparantly is meant to enhance ab stability. I found large edge effects on my plates when using these techniques. Thanks for your help ---------------------------------------------------------------------- Disclaimer: The above is a personal opinion and does not reflect the official view of AgResearch Ltd. ---------------------------------------------------------------------- From owner-diagnostics@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!biosci!not-for-mail From: mawolfe@merle.acns.nwu.edu (Miriam Wolfe) Newsgroups: alt.med.equipment,alt.practitioners,alt.sci.mbe,bionet.biophysics,bionet.cellbiol,bionet.diagnostics,bionet.microbiology,bionet.virology,biz.general Subject: wtb: MICROSCOPES Date: 20 Dec 1995 19:17:37 -0800 Organization: Family Studies Laboratory Lines: 7 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biophysics:1531 bionet.cellbiol:3689 bionet.diagnostics:494 bionet.microbiology:4324 bionet.virology:5315 Two doctors are looking to buy (cheap, relatively speaking) two working light microscopes for their office to check out tissue samples. Please reply via e-mail, Miriam Wolfe mawolfe@merle.acns.nwu.edu From owner-diagnostics@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!nntp.coast.net!col.hp.com!csn!magnus.acs.ohio-state.edu!slip3-21.acs.ohio-state.edu!lboros From: lboros@magnus.acs.ohio-state.edu (Laszlo G. Boros) Newsgroups: bionet.diagnostics Subject: amylin receptor binding assay Date: Thu, 21 Dec 1995 14:09:15 GMT Organization: The Ohio State University Lines: 20 Message-ID: NNTP-Posting-Host: slip3-21.acs.ohio-state.edu Keywords: amylin X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] We are trying to establish a competitive binding receptor assay for amylin (pancreatic islet polypeptide) on pancreatic carcinoma cell lines in our laboratory. We are using 5000 uCi labeled amylin in each experiment and we add 10-5, 10-6, 10-7, 10-8, 10-9, 10-10, 10-11, 10-12 and 10-13 uM cold, unlabeled peptide as competitors, separately. The results are the opposite what we expect from the assay. The highest concentration of cold peptide gives us the highest hot binding, decreasing concentrations of the cold peptide results in decreasing hot peptide binding in the assay. This is the opposite what we have seen with other peptides where high cc of cold peptide resulted low hot binding because of competition for the binding sites. We have contemplated the idea that amylin might have an internal receptor and competition is occurs not at the cell surface where the unbound hot peptide gets washed off easily after the experiment, or amylin binding is ot specific to receptors but the osmotic pressure will direct the hot peptide to the cell surface or into the cells. If anyone ever saw the same binding pattern in their experiment, comments are welcomed and very much appreciated. Thanks, Laszlo G. Boros, MD The Ohio State University Dept. of Surgery. From owner-diagnostics@net.bio.net Wed Dec 20 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!news.dacom.co.kr!news.uoregon.edu!news.rediris.es!news.cica.es!antonioo From: BIOTEC@GOLIAT.UGR.ES Newsgroups: bionet.diagnostics Subject: ANNOUNCE: II COURSE OF TROPICAL MEDICINE AND PARASITOLOGY UNESCO CHAIR Date: 21 Dec 1995 16:32:27 GMT Organization: UNIVERSIDAD DE GRANADA Lines: 23 Distribution: world Message-ID: <4bcnh7$a70_002@news.cica.es> NNTP-Posting-Host: parasito.ugr.es X-Newsreader: News Xpress Version 1.0 Beta #3 II Course on Tropical Medicine and Parasitology (Unesco Chair)in the University of Granada (Spain), is announced for May 1996. Intensive Course (120 hours), designed for post-graduates seeking updating in Tropical Medicine and Parasitology, provided by internationally recognized specialists. Basic contents: Clinical epidemiology. Malnutrition and disease. Detailed study of more important tropical diseases (helminths, protozoa, fungi, bacteria and viruses) with special emphasis on epidemiological, clinical and diagnostic aspects. Tuition Fees: 100.000 Pesetas Students from undeveloped countries free. Management: Vicerrectorado de Investigacion y Relaciones Internacionales. Universidad de Granada Hospital Real 18071 Granada Spain Phone. 34 58 243009 Fax. 34 58 244312 E-mail. inrel@ugr.es From owner-diagnostics@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!news.sprintlink.net!earth.superlink.net!usenet From: E-Man Newsgroups: bionet.diagnostics Subject: Re: Video/printers Date: Thu, 21 Dec 1995 21:33:20 -0500 Organization: Morales Enterprises Lines: 24 Message-ID: <30DA18F0.1351@mars.superlink.net> References: <199512120950.BAA07155@net.bio.net> NNTP-Posting-Host: sb6.superlink.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (Win95; I) Vigfrid Ness wrote: > > Hi! > Have any one of you any good brand name for video chameras and black/white > thermoprinters to use for the recording of gels etc.???? > > Vigfrid Ness > Vigfrid Ness > DYNAL A.S. > Norway > > E.mail: vigfrid.ness@veths.no Have you considered using digital cameras (e.g. Casio, Nikon)for the recording of scientific data?? You could then print out the images on a printer of your choice and still have a copy of the image on disk or tape. Elliot. -- ====================Morales Enterprises===================== Elliot Morales email: emorales@mars.superlink.net "Specializing in abuse, humiliation, & money-making schemes" From owner-diagnostics@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!rug.ac.be!mario.vaneechoutte From: mario.vaneechoutte@rug.ac.be Newsgroups: bionet.diagnostics Subject: (Fwd) Re: Video/printers Date: 21 Dec 1995 23:15:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512220713.AA00811@allserv.rug.ac.be> NNTP-Posting-Host: net.bio.net >>Vigfrid Ness wrote: >> >> Hi! >> Have any one of you any good brand name for video chameras and black/white >> thermoprinters to use for the recording of gels etc.???? >> >> Vigfrid Ness >> E.mail: vigfrid.ness@veths.no >Have you considered using digital cameras (e.g. Casio, Nikon)for the recording >of scientific data?? You could then print out the images on a printer of your >choice and still have a copy of the image on disk or tape. >Elliot Morales email: emorales@mars.superlink.net We have been considering purchase of a digital camera (of Canon) but were informed that sensitivity for recording ethidium bromide stained gels was too low. Digital camera's could indeed be very convenient to replace Polaroid camera's: less waste, less loss of resolution, immediate digitalisation (i.e. no need for scanning the gels), cheaper. Video camera's still are expensive. If someone knows about useful digital camera devices, let us know. Mario Vaneechoutte University Hospital Ghent, Belgium From owner-diagnostics@net.bio.net Thu Dec 21 22:00:00 1995 Path: biosci!BEPC2.IHEP.AC.CN!wangyq From: wangyq@BEPC2.IHEP.AC.CN Newsgroups: bionet.diagnostics Subject: Cancer_Screening Date: 22 Dec 1995 08:10:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <0099B453.817760A0.217@bepc2.ihep.ac.cn> NNTP-Posting-Host: net.bio.net Dear Netters: I want to cooperate with a hospital to do a cancer screening project in Beijing. Could you please recommend some immunodiagnostic kits to me for this purpose such as CA-50, PSA, etc, or maybe you know better kits with higher sensitivity? And do you know the source and price of chemical ice bricks that can maintain at 4-5 centigree for 14 to 15 hours? Thank you. Best Regards, Wang Yuquan Beijing Cons Bio-Tech From owner-diagnostics@net.bio.net Fri Dec 22 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!user From: filter@firthcom.demon.co.uk (Steve Firth) Newsgroups: bionet.diagnostics Subject: Re: (Fwd) Re: Video/printers Date: Sat, 23 Dec 1995 22:26:41 +0000 Organization: Firth Consulting Lines: 40 Message-ID: References: <9512220713.AA00811@allserv.rug.ac.be> NNTP-Posting-Host: firthcom.demon.co.uk X-NNTP-Posting-Host: firthcom.demon.co.uk In article <9512220713.AA00811@allserv.rug.ac.be>, mario.vaneechoutte@rug.ac.be wrote: >Digital camera's could indeed be very convenient to >replace Polaroid camera's: >less waste, Probably. > less loss of resolution, No, sorry most digital cameras and certainly al video cameras give you musch worse resolution than a Polaroid print. >immediate digitalisation (i.e. no need for scanning the gels), cheaper. Only cheaper on the consumables and you'd have to do a heck of a lot of work to make it worthwhile. >Video camera's still are expensive. > And low resolution, even S-VHS/Hi-8 only gives a resolution of around 756x520 pixels, and this is pushing the limits. >If someone knows about useful digital camera devices, let us know. A Leaf Lumina would give you good resolution for static subjects and has good resolution, but the price is still very high (over GBP 2500) and the camera needs to take long exposures so only static subjects are worthwhile. In my own experiments, I had reasonable results using a single pass desktop transparency scanner capable of scanning 36bits per pixel at a resolution of 2400 pixels per inch. This is good for stained, dried polyacrylamide gels but of no use for ethidium bromide stained gels. The 36bpp referred to above is not a misprint, these scanners are built to overscan the image to improve ability to resolve images with a high contrast. From owner-diagnostics@net.bio.net Sun Dec 24 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!news.iij.ad.jp!fu.bekkoame.or.jp!suika!usenet From: Takahiko Ishiguro Newsgroups: bionet.diagnostics Subject: Re: (Fwd) Re: PCR KIT-> NO GELLS, Quantitative, Double Fidelity Date: Wed, 20 Dec 1995 18:44:59 +0900 Organization: Tosoh Corporation Lines: 17 Message-ID: <30D7DB1B.18DC@ppp.bekkoame.or.jp> References: <199512080949.JAA19528@caird.scri.sari.ac.uk> NNTP-Posting-Host: yhm0074.bekkoame.or.jp Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 2.0b3 (Win95; I) To: D Jones Hi; At a glance, this seems to be pretty interesting,but the end-point quantification of PCR products cannot always bring to the estimation of a starting template number, principally, because the PCR kinetics is not on the exponential amplification schema, which is usually believed. If you would be interested in this area, I am happy to show you my article, ANALYTICAL BIOCHEMISTRY 229,207-213,1995. Takahiko ISHIGURO, Ph.D. TOSOH CORPORATION Tokyo Research Laboratories Kanagawa, JAPAN E-mail:isiguro@tosoh.co.jp -- MZ From owner-diagnostics@net.bio.net Mon Dec 25 22:00:00 1995 Path: biosci!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!news.sprintlink.net!sparky.insinc.net!grumpy.insinc.net!limestone.kosone.com!usenet From: "richard d. eng" Newsgroups: bionet.diagnostics Subject: RFI - Norwalk Virus Direct Test Date: Tue, 26 Dec 1995 12:53:26 -0500 Organization: Ontario Ministry of Health Lines: 5 Message-ID: <30E03696.60B0@limestone.kosone.com> NNTP-Posting-Host: ki-ppp3.kosone.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (Win95; I) I know the genome of Norwalk Virus has been identified. EM, a labour intensive test, is the only test available for us. There are many outbreaks in the winter due to this agent. Is there a commercially availabe direct test out there? EIA, PCR? A cost effective direct test for this agent is much needed. From owner-diagnostics@net.bio.net Wed Dec 27 22:00:00 1995 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Thermostable enzymes Date: 28 Dec 1995 05:52:12 -0800 Organization: SASA Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9512281347.aa06620@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net PCR and LCR are based on thermostable DNA polymerase and ligase respectively. In the dog days between Christmas and New Year I find myself wondering what other sequence-specific amplification methods could be devised if suitable enzymes were available. Can anyone tell me if there is, for example, a thermostable RNA polymerase commercially available? It seems to me that this would greatly simplify 3SR/NASBA. Input/ other ideas welcomed. Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Thu Dec 28 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!news3.digex.net!access1.digex.net!rschifre From: Richard Schifreen Newsgroups: bionet.diagnostics Subject: Re: Thermostable enzymes Date: Fri, 29 Dec 1995 10:05:00 -0500 Organization: Express Access Online Communications, USA Lines: 35 Message-ID: References: <9512281347.aa06620@jura.sasa.gov.uk> NNTP-Posting-Host: access1.digex.net Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <9512281347.aa06620@jura.sasa.gov.uk> You are absolutely right as to the utility of thermostable enzymes for working with RNA; and that there currently aren't any available. We (Life Technologies/ GIBCO BRL) are trying to engineer a thermostable reverse transcriptase. So far, we're still trying. If you'd like, I'd be glad to put you in contact with the project leader. Have a great 1996. Rich Schifreen phone: 301-840-4163 Life Technologies fax: 301-670-1493 E-Mail: rschifre@access.digex.net On 28 Dec 1995, Dr Kevin O'Donnell wrote: > PCR and LCR are based on thermostable DNA polymerase and ligase > respectively. In the dog days between Christmas and New Year I find > myself wondering what other sequence-specific amplification methods > could be devised if suitable enzymes were available. Can anyone tell me > if there is, for example, a thermostable RNA polymerase commercially > available? It seems to me that this would greatly simplify > 3SR/NASBA. > > Input/ other ideas welcomed. > > Kevin > > > Dr Kevin O'Donnell "Work as if you were in the early days > Diagnostics and Molecular Biology of a better nation" > SASA - Alasdair Gray > Edinburgh > > From owner-diagnostics@net.bio.net Fri Dec 29 22:00:00 1995 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news2.new-york.net!not-for-mail From: Steve Grenard Subject: FALSE POSITIVE PPD Content-Type: text/plain; charset=us-ascii X-Nntp-Posting-User: (Unauthenticated) Content-Transfer-Encoding: 7bit Organization: Herpmed Communications Message-ID: X-Mailer: Mozilla 1.1PE (Windows; I; 16bit) Mime-Version: 1.0 X-Nntp-Posting-Host: herpmed.com Date: Sat, 30 Dec 1995 05:26:47 GMT Lines: 11 I am interested in finding out if a patient is mistakenly given two PPD tests perhaps a day or so apart or a few hours apart if they will cross-react and cause one or both to be falsely positive? Please respond by e-mail to: Steve Grenard grenard@herpmed.com Thank you. From owner-diagnostics@net.bio.net Sat Dec 30 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!news.ultranet.com!usenet From: "Carl M. Good III" Newsgroups: bionet.diagnostics Subject: pyranose oxidase Date: Sun, 31 Dec 1995 18:47:47 -0500 Organization: UltraNet Communications, Inc. Lines: 12 Message-ID: <30E72123.670E@ultranet.com> NNTP-Posting-Host: itp.ultranet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (Win95; I) We are seeking a supplier of pyranose oxidase. There may be a supplier in Japan. If anyone knows of a supplier I would appreciate a reply. Thanks very much -- __________________________________ Dr. Carl M. Good III 560 Longley Rd., Groton MA 01450 carlg@ultranet.com http://www.ultranet.com/~carlg/ __________________________________