From owner-diagnostics@net.bio.net Mon Jan 01 22:00:00 1996 Newsgroups: bionet.diagnostics Path: biosci!agate!ihnp4.ucsd.edu!munnari.OZ.AU!news.mel.connect.com.au!yarrina.connect.com.au!warrane.connect.com.au!news.gil.com.au!ppp14.ipswich.gil.com.au!msryan From: msryan@gil.ipswichcity.qld.gov.au (Mike Ryan) Subject: Source of Antibodies and Antigens Sender: news@gil.com.au Message-ID: Date: Tue, 2 Jan 1996 22:47:14 GMT X-Nntp-Posting-Host: ppp14.ipswich.gil.com.au Organization: GRAPHIC SCIENTIFIC X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Keywords: Rubella, Toxoplasma, CMV Lines: 8 I am looking for a source of purified antigen for CMV, Toxoplasma, Rubella to coat microtitre plates to develop ELISA tests. I am also looking for a source of secondary antibodies - anti human IgG and anti human IgM labelled with Horseradish Peroxidase. Finally, I am also seeking a source of Rabbit anti-human IgM Could anyone help me to finf a reliable supply for these item? Thank you in advance. From owner-diagnostics@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!aol.com!JPowers521 From: JPowers521@aol.com Newsgroups: bionet.diagnostics Subject: suppliers of disease state serum and purified infectious agents Date: 2 Jan 1996 08:42:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <960102114040_29579576@emout06.mail.aol.com> NNTP-Posting-Host: net.bio.net Hello, I am looking for a supply of positive human serum for the following agents: T. Cruzi (Chagas' Disease) Visceral Leishmaniasis Dengue Fever (IgM positive) You can contact me at the following numbers: Janice Powers Capricorn Products Ph 617-646-6663 FAX 617-646-7071 email: JPowers521@aol.com Tnanks for your help. Janice From owner-diagnostics@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!bangslabs.com!Leigh From: Leigh@bangslabs.com ("Leigh") Newsgroups: bionet.diagnostics Subject: Silica gel pillows Date: 2 Jan 1996 09:25:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Subject: Time: 10:08 OFFICE MEMO Silica gel pillows Date: 1/2/96 Can anyone tell me a source of those little silica gel dessicant pillows which one can use to keep kits dry. I know that they are available for 1-10 cents (US$0.01-0.10) each depending source. A friend has found a 10 cent source but can't find lower prices, so far. Any help will be appreciated. Leigh Bangs, aka "The Particle Doctor"; leigh@bangslabs.com Bangs Laboratories, Inc., 979 Keystone Way, Carmel, IN 46032-2823 USA Tel: 317-844-7176 Fax: 317-575-8801 "The Microsphere Zone" [Web Home Page: http://www.bangslabs.com/blab] From owner-diagnostics@net.bio.net Mon Jan 01 22:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!news.sprintlink.net!newsfeed.internetmci.com!info.ucla.edu!news.bc.net!rover.ucs.ualberta.ca!duckter.glaxo.med.ualberta.ca!user From: tyr-2@bones.biochem.ualberta.ca (Karl Fischer) Newsgroups: bionet.diagnostics Subject: Re: Source of Antibodies and Antigens Date: Tue, 02 Jan 1996 11:53:38 -0700 Organization: Med. Microbiology, Univ. of Alberta Lines: 25 Message-ID: References: NNTP-Posting-Host: duckter.glaxo.med.ualberta.ca X-Newsreader: Yet Another NewsWatcher 2.1.5 In article , msryan@gil.ipswichcity.qld.gov.au (Mike Ryan) wrote: > I am looking for a source of purified antigen for CMV, Toxoplasma, Rubella to > coat microtitre plates to develop ELISA tests. Try Chemicon International, Inc. 1-800-437-7500 (tel) 1-909-676-9209 (fax) Rubella cat# AG879 T. gondii (RH) cat# AG859 CMV (AD-169) cat# AG843 Usual disclaimers apply. Cheers Karl the hepB guy -- Karl Fischer tyr-2@bones.biochem.ualberta.ca From owner-diagnostics@net.bio.net Tue Jan 02 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 3 Jan 1996 02:00:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601031000.CAA21820@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!news.dacom.co.kr!usenet From: marcop95@bora.dacom.co.kr (Lee Sang Chan) Newsgroups: bionet.diagnostics Subject: Wanna know magazine and websites for new diagnostic test products in U.S.A Date: Thu, 04 Jan 1996 14:36:01 GMT Organization: DACOM Internet Lines: 16 Message-ID: <4cgocv$as9@nis.dacom.co.kr> NNTP-Posting-Host: bora.dacom.co.kr X-Newsreader: Forte Free Agent 1.0.82 I'd like to know magazines and websites for New diagnostic test products (instrument and reagent) in U.S.A. I'm with diagnostic reagent department of Choong-Wae Pharma coporation. I appreciate any reply soon. Lee Sang Chan, Product manager, Diagnostic reagent department, Choong-Wae Pharma Coporation, Seoul. Korea. marcop95@bora.dacom.co.kr From owner-diagnostics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!aol.com!MHughes77 From: MHughes77@aol.com Newsgroups: bionet.diagnostics Subject: Re: Silica gel desiccant packs Date: 3 Jan 1996 18:18:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <960103211533_31139432@mail02.mail.aol.com> NNTP-Posting-Host: net.bio.net In a message dated 96-01-02 12:38:49 EST, Leigh Bangs asked: > Can anyone tell me a source of those little silica gel dessicant pillows > which one can use to keep kits dry. I know that they are available for > 1-10 cents (US$0.01-0.10) each depending source. > A friend has found a 10 cent source but can't find lower prices, so far. > Any help will be appreciated. Try Impak in Pasadena, California USA phone 818-398-7300 fax 818-398-7400 They sell 1/2 gram packs for 3-4 cents each in gallon cans. Mark Hughes mhughes77@aol.com From owner-diagnostics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.emi.com!pauling.wadsworth.org!usenet From: bobrej@rachel.albany.edu (Robert Rej) Newsgroups: bionet.diagnostics Subject: Conference on Drugs and Toxins Date: 4 Jan 1996 22:57:39 GMT Organization: SUNY Albany School of Public Health Lines: 100 Message-ID: <4chm13$pv7@pauling.wadsworth.org> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: WinVN 0.92.3 ARNOLD O. BECKMAN CONFERENCE Drugs and Toxins in Clinical Laboratory Practice February 4 - 5, 1996--San Diego, CA ----------------------------------------------------------------- SUNDAY, FEBRUARY 4, 1996 7:00amRegistration OPENING SESSION 8:15amWelcome & Opening Remarks Robert Rej, Ph.D., Committee Chairman 8:30amClinical Assessment of Drug Toxicity and Safety in Humans Wayne Ray, Ph.D., Vanderbilt University Medical Center, Nashville, Tennessee SESSION I - TOXIC SIDE EFFECTS IN THERAPY Moderator: Mario Werner, M.D. 9:15amTitrating Cardiovascular Drugs Frank I. Marcus, M.D., University of Arizona College of Medicine, Tucson, Arizona 10:00amBreak 10:30amToxic Effects of Immunosuppressive Drugs: Mechanisms and Strategies for Controlling Them Leslie M. Shaw, Ph.D., University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 11:15amToxicity of Antineoplastic Drugs Bruce Chabner, M.D., Massachusetts General Hospital, Boston, Massachusetts 12:00noonLunch SESSION II - EPIDEMIOLOGICAL AND LEGAL ASPECTS OF DRUGS OF ABUSE Moderator: Edward R. Ashwood, M.D. 1:30pmEpidemiology of Illicit and Abused Drugs in Arrestees and Emergency Department Patients Compared to the General Population Beatrice A. Rouse, Ph.D., U.S. Dept of Health and Human Services, Rockville, Maryland 2:15pmThe Role of the Clinical Toxicologist in Court R. Thomas Chamberlain, J.D., Ph.D., SmithKline Beecham Clinical Laboratories, Tampa, Florida S. 3:00pmBreak 3:30pm-5:00pmCase Studies 6:00pm-7:00pmReception MONDAY, FEBRUARY 5, 1996 SESSION III - TOXICITY AND CHRONIC EXPOSURE Moderator: Robert Rej, Ph.D. 8:00amThe Health Consequences of Short- and Long-Term Postmenopausal Hormone Therapy Noel S. Weiss, M.D., Ph.D., University of Washington, Seattle, Washington 8:45amLong-Term Exposure to Environmental Contaminants: Is There an Impact on Human Health? Jay B. Silkworth, Ph.D., General Electric Corporation, Schenectady, New York 9:30amBreak 10:00amThe Cause and Prevention of Degenerative Diseases: Which Toxins Matter? Bruce N. Ames, Ph.D., University of California, Berkeley, California 11:00am-12:30pmCase Studies 12:30pmLunch (on own) SESSION IV - EMERGENCY TOXICOLOGY Moderator: Martin H. Kroll, M.D. 2:00pmToxic Syndromes Horace K. Liang, M.D., Johns Hopkins Hospital, Baltimore, Maryland 2:45pmPediatric Poisonings Julius Goepp, M.D., Johns Hopkins Hospital, Baltimore, Maryland 3:30pmBreak 4:00pmAdvances in Poison Management, Toxic Syndromes, and Drugs of Abuse Marc J. Bayer, M.D., University of Connecticut Health Center, Farmington, Connecticut 4:45pm-5:00pmClosing Remarks CONTINUING MEDICAL EDUCATION AACC is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to sponsor Continuing Medical Education for physicians. The AACC designates this continuing medical education activity for 12 credit hours in Category 1 of the Physician's Recognition Award of the American Medical Association. This program is approved by the AACC for 12 Category 1 ACCENT(r) credit hours toward the Clinical Chemists' Recognition Award. ACCOMMODATIONS A block of rooms is being held for conference attendees at the Wyndham Emerald Plaza, San Diego, California. Located at the heart of San Diego's vibrant, new downtown, The Wyndham offers guests immediate access to all the city has to offer. Shopping, dining and nightclubs are within walking distance. The San Diego Zoo, Sea World, Old Town and many other attractions are only a 5-10 minute drive. The special conference rate is $129 for a single room and $144 for a double room. Reservations may be made by calling the Wyndham at 619-239-4500. Identify yourself as attending the AACC Beckman Conference to reserve your room at the special rate. Rooms must be reserved by From owner-diagnostics@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.emi.com!pauling.wadsworth.org!usenet From: bobrej@rachel.albany.edu (Robert Rej) Newsgroups: bionet.diagnostics Subject: Corrected Post: Conference on Drugs & Toxins Date: 4 Jan 1996 22:59:57 GMT Organization: SUNY Albany School of Public Health Lines: 114 Message-ID: <4chm5d$pv9@pauling.wadsworth.org> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: WinVN 0.92.3 ARNOLD O. BECKMAN CONFERENCE Drugs and Toxins in Clinical Laboratory Practice February 4 - 5, 1996--San Diego, CA ----------------------------------------------------------------- SUNDAY, FEBRUARY 4, 1996 7:00amRegistration OPENING SESSION 8:15amWelcome & Opening Remarks Robert Rej, Ph.D., Committee Chairman 8:30amClinical Assessment of Drug Toxicity and Safety in Humans Wayne Ray, Ph.D., Vanderbilt University Medical Center, Nashville, Tennessee SESSION I - TOXIC SIDE EFFECTS IN THERAPY Moderator: Mario Werner, M.D. 9:15amTitrating Cardiovascular Drugs Frank I. Marcus, M.D., University of Arizona College of Medicine, Tucson, Arizona 10:00amBreak 10:30amToxic Effects of Immunosuppressive Drugs: Mechanisms and Strategies for Controlling Them Leslie M. Shaw, Ph.D., University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 11:15amToxicity of Antineoplastic Drugs Bruce Chabner, M.D., Massachusetts General Hospital, Boston, Massachusetts 12:00noonLunch SESSION II - EPIDEMIOLOGICAL AND LEGAL ASPECTS OF DRUGS OF ABUSE Moderator: Edward R. Ashwood, M.D. 1:30pmEpidemiology of Illicit and Abused Drugs in Arrestees and Emergency Department Patients Compared to the General Population Beatrice A. Rouse, Ph.D., U.S. Dept of Health and Human Services, Rockville, Maryland 2:15pmThe Role of the Clinical Toxicologist in Court R. Thomas Chamberlain, J.D., Ph.D., SmithKline Beecham Clinical Laboratories, Tampa, Florida S. 3:00pmBreak 3:30pm-5:00pmCase Studies 6:00pm-7:00pmReception MONDAY, FEBRUARY 5, 1996 SESSION III - TOXICITY AND CHRONIC EXPOSURE Moderator: Robert Rej, Ph.D. 8:00amThe Health Consequences of Short- and Long-Term Postmenopausal Hormone Therapy Noel S. Weiss, M.D., Ph.D., University of Washington, Seattle, Washington 8:45amLong-Term Exposure to Environmental Contaminants: Is There an Impact on Human Health? Jay B. Silkworth, Ph.D., General Electric Corporation, Schenectady, New York 9:30amBreak 10:00amThe Cause and Prevention of Degenerative Diseases: Which Toxins Matter? Bruce N. Ames, Ph.D., University of California, Berkeley, California 11:00am-12:30pmCase Studies 12:30pmLunch (on own) SESSION IV - EMERGENCY TOXICOLOGY Moderator: Martin H. Kroll, M.D. 2:00pmToxic Syndromes Horace K. Liang, M.D., Johns Hopkins Hospital, Baltimore, Maryland 2:45pmPediatric Poisonings Julius Goepp, M.D., Johns Hopkins Hospital, Baltimore, Maryland 3:30pmBreak 4:00pmAdvances in Poison Management, Toxic Syndromes, and Drugs of Abuse Marc J. Bayer, M.D., University of Connecticut Health Center, Farmington, Connecticut 4:45pm-5:00pmClosing Remarks CONTINUING MEDICAL EDUCATION AACC is accredited by the Accreditation Council for Continuing Medical Education (ACCME) to sponsor Continuing Medical Education for physicians. The AACC designates this continuing medical education activity for 12 credit hours in Category 1 of the Physician's Recognition Award of the American Medical Association. This program is approved by the AACC for 12 Category 1 ACCENT(r) credit hours toward the Clinical Chemists' Recognition Award. ACCOMMODATIONS A block of rooms is being held for conference attendees at the Wyndham Emerald Plaza, San Diego, California. Located at the heart of San Diego's vibrant, new downtown, The Wyndham offers guests immediate access to all the city has to offer. Shopping, dining and nightclubs are within walking distance. The San Diego Zoo, Sea World, Old Town and many other attractions are only a 5-10 minute drive. The special conference rate is $129 for a single room and $144 for a double room. Reservations may be made by calling the Wyndham at 619-239-4500. Identify yourself as attending the AACC Beckman Conference to reserve your room at the special rate. Rooms must be reserved by January 10, 1996 to ensure availability. REGISTRATION Prior to January 16, the registration fee is $350 for AACC members and $400 for non-members. After January 16, all fees are increased by $50. Please contact AACC Customer Service (see below) for a registration brochure. FOR ADDITIONAL INFORMATION, CONTACT: AACC Customer Service 2101 L Street, NW, Suite 202 Washington, DC 20037-1526 800-892-1400, 202-857-0717, FAX: 202-833-4576 From owner-diagnostics@net.bio.net Fri Jan 05 22:00:00 1996 Path: biosci!SHANI.NET!ffish From: ffish@SHANI.NET (Falk Fish) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: 5 Jan 1996 22:03:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Orgenics LTD (Yavne, Israel) offers a saliva HIV ImmunoComb test kit, intended for laboratory or alternate sites (not a home kit). Sensitivity and specificity is as good as all leading HIV serology products. A large clinical trial, conducted with the Ministry of Health, indicated that sometimes saliva converts to positive before serum or plasma do. Orgenics can be reached at orgenics@elronet.co.il or: Tel: int+972-8-9438752; Fax: int+972-8-9438758 Falk Fish, Tel-Aviv, Israel From owner-diagnostics@net.bio.net Sat Jan 06 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.ultranet.com!usenet From: "Carl M. Good III" Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: Sat, 06 Jan 1996 20:26:30 -0500 Organization: UltraNet Communications, Inc. Lines: 23 Message-ID: <30EF2146.4AB7@ultranet.com> References: NNTP-Posting-Host: itp.ultranet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b3 (Win95; I) Falk Fish wrote: > > Orgenics LTD (Yavne, Israel) offers a saliva HIV ImmunoComb test kit, > intended for laboratory or alternate sites (not a home kit). Sensitivity > and specificity is as good as all leading HIV serology products. > > A large clinical trial, conducted with the Ministry of Health, indicated > that sometimes saliva converts to positive before serum or plasma do. > > Orgenics can be reached at orgenics@elronet.co.il or: > Tel: int+972-8-9438752; Fax: int+972-8-9438758 > > Falk Fish, Tel-Aviv, Israel -- Has this been compaired to the Epitope test? __________________________________ Dr. Carl M. Good III 560 Longley Rd., Groton MA 01450 carlg@ultranet.com http://www.ultranet.com/~carlg/ __________________________________ From owner-diagnostics@net.bio.net Sat Jan 06 22:00:00 1996 Path: biosci!agate!news.duke.edu!solaris.cc.vt.edu!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rlh115@aol.com (RLH115) Newsgroups: bionet.diagnostics Subject: help with research project related to antibodies Date: 6 Jan 1996 22:35:50 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 21 Sender: root@newsbf02.news.aol.com Message-ID: <4cnf2m$alt@newsbf02.news.aol.com> Reply-To: rlh115@aol.com (RLH115) NNTP-Posting-Host: newsbf02.mail.aol.com am presently a student (with a biological research background) in a masters degree program in business administration. I am conducting a market survey to be used for a research paper to complete my degree requirements. The short one page questionaire examines the use of antibodies in all of the biological science fields. I would greatly appreciate the help of any individual who currently uses any antibodies for any reason to quickly take my questionaire. Since I am a student, I am unable to compensate anyone for their response. All individual information will be confidential. However, I am willing to provide any participant who requests it with a summary of the results upon completion of the project. If you are willing to assist me on my project, please e-mail me with a short request- " send survey" to: rlh115@aol.com Thank you for your time. R. Henderson The Pennsylvania State University R. Henderson The Pennsylvania State University From owner-diagnostics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!in2.uu.net!newsflash.concordia.ca!news.nstn.ca!news.cs.indiana.edu!umn.edu!newsstand.tc.umn.edu!gold.tc.umn.edu!hydex004 From: hydex004@gold.tc.umn.edu (Frederick W Hyde) Newsgroups: bionet.diagnostics Subject: Re: Saliva Rapid HIV Test Announced Date: 8 Jan 1996 09:23:17 -0600 Organization: University of Minnesota Lines: 14 Message-ID: References: NNTP-Posting-Host: gold.tc.umn.edu ffish@SHANI.NET (Falk Fish) writes: >Orgenics LTD (Yavne, Israel) offers a saliva HIV ImmunoComb test kit, >intended for laboratory or alternate sites (not a home kit). Sensitivity >and specificity is as good as all leading HIV serology products. >A large clinical trial, conducted with the Ministry of Health, indicated >that sometimes saliva converts to positive before serum or plasma do. How does this compare with the test that Epitope offers using its Orasure device??? Fred From owner-diagnostics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!biosci!not-for-mail From: phi94fpi@hpboot1.rz.uni-leipzig.de (i.V. Ralf Hofestaedt) Newsgroups: de.sci.medizin,de.sci.biologie,bionet.cellbiol,bionet.diagnostics,bionet.genome.chromosomes,bionet.info-theory,bionet.metabolic-reg,bionet.molbio.embldatabank Subject: Conference on Bioinformatics call for papers Date: 8 Jan 1996 12:10:44 -0800 Organization: University of Leipzig, Germany Lines: 150 Sender: daemon@net.bio.net Distribution: world Message-ID: <4crri1$npg@server2.rz.uni-leipzig.de> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.cellbiol:3752 bionet.diagnostics:521 bionet.genome.chromosomes:990 bionet.info-theory:3852 bionet.metabolic-reg:649 bionet.molbio.embldatabank:590 The following is a call for papers for the German Conference on Computer Science and Biology If you need an earlier decision on your submitted paper, so you can make your plans, please let us know. We could provide an early decision, although the partition of accepted abstracts into oral presentation and posters will take place only after June 15. If you need early acceptance, please submit your abstract by April 20. FIRST ANNOUNCEMENT COMPUTER SCIENCE AND BIOLOGY German Conference on B I O I N F O R M A T I C S (GCB`96) Leipzig, September 30th - October 2nd, 1996 The German Conference on Bioinformatics is organized on behalf of FG 4.0.2 Informatik in den Biowissenschaften of the German Society of Computer Science (GI) in cooperation with the AG of Computereinsatz in den Biowissenschaften of the German Society of Chemical Technique and Biotechnology (DECHEMA) and the AG Mathematische Modelle in Biologie und Medizin of the German Society of Medical Informatics, Statistics and Epidemiology (GMDS). The conference will take place at the University of Leipzig, September 30th - October 2nd, 1996, and is intended to bring together scientists who are addressing problems in biosciences and medicine using advanced computational methods including data modeling, simulation, artificial intelligence, computer graphics (visualization), robotics, combinatorial and stochastic optimization. The conference is concerned with all aspects combining computer science and biosciences. Topics of particular interest include, but are not limited to: - Genome Analysis - Models of Gene Regulation - Formal Languages and DNA - Molecular Docking and Recognition - Molecular Modeling and Protein Design - Models of Pattern and Structure Formation - Models in Cell Biology - Models of Dynamic Biological Systems - Self-Organization and Complex Systems - Metabolic Engineering - Metabolic Pathways - High Performance Computing - Biological Database Technology - Visualization and Animation of Biological Processes - Artificial Intelligence and Complex Systems - Evolutionary Computing - DNA Computing - Biological Paradigms in Computer Science Proceedings Extended abstracts of all accepted presentations including posters and computerdemos will be published in the conference abstract book which will be distributed to all participants. It is planned that full length papers will be invited by the PC for publication by Springer-Verlag "Lecture Notes of Computer Science" after the conference. Submission Procedures The closing date for receiving papers (extended abstracts of 3 pages), posters and computer demos (abstracts of 1 page) is May 1, 1996. The conference offers the possibility for the presentation of tutorials (abstracts of 1 page). The decision on acceptance for presentation will be communicated by June 15, 1996. Authors are urged to specify the category to which they are submitting their paper. Submissions must be written in English and should include title, author's name, mailing address, telephone number, fax number, email address and a list of keywords. They should be sent to: PD Dr. R. Hofestaedt Tel. 0341 / 9716100 Prof. Dr. M. Loeffler Fax 0341 / 9716109 University Leipzig email: GCB96@imise.uni-leipzig.de Department of Medical Informatics, Statistics and Epidemiology Liebigstr. 27 04103 Leipzig Organizing Committee R. Hofestaedt (Leipzig & Koblenz, GI-FG, Germany) T. Lengauer (Bonn, GI-FG, Germany) M. Loeffler (Leipzig, GMDS, Germany) D. Schomburg (Braunschweig, DECHEMA, Germany) Program Comittee J. Collado-Vides (Mexico) M. Mewes (Germany) A. Danchin (France) J. Shavlik (USA) A. Dress (Germany) S. Suhai (Germany) P. Karp (USA) M. Vingron (Germany) H. Kubinyi (Germany) E. Wingender (Germany) H. Lim (USA) H. Zima (Austria) M. Mavrovouniotis (USA) Important Dates Deadline of Submission: May 1, 1996 Notification of Acceptance: June 15, 1996 Receipt of Camera Ready Manuscript August 1, 1996 Registration Fees Full participation 250 DM GI, GMDS, DECHEMA 200 DM Student 80 DM One day registration 90 DM Includes: a copy of the proceedings, tea/coffee at the conference and food and drink at the poster session. ___________________________________________________________________ Registration form (to send preferably by email): GCB`96 Universitaet Leipzig Institut fuer Medizinische Informatik Phone 0341 / 9716100 und Statistik Fax 0341 / 9716109 Liebigstr. 27 email: GCB96@imise.uni-leipzig.de 04103 Leipzig Name: ____________________________________________ Institution: ____________________________________________ Address: ____________________________________________ Email: ____________________________________________ Tel. /Fax: ____________________________________________ I would like to participate in GCB`96 ( ) yes ( ) no ORAL ( ) POSTER ( ) COMPUTERDEMO ( ) From owner-diagnostics@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!UNL.EDU!rdonis From: rdonis@UNL.EDU (Ruben Donis) Newsgroups: bionet.diagnostics Subject: International BVD Virus Symposium, Announcement I Date: 8 Jan 1996 14:28:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 88 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net First Announcement ************************************************************************** INTERNATIONAL SYMPOSIUM 50 YEARS BOVINE VIRAL DIARRHEA VIRUS CORNELL UNIVERSITY June 23-25, 1996 COLLEGE OF VETERINARY MEDICINE CORNELL UNIVERSITY In recognition of the 50th anniversary of the "discovery" of a cattle disease that is now known to be caused by BVD virus, an international symposium will convene at Cornell University on June 23-25, 1996. The intent of the symposium is to review the past 50 years of BVD research ant to attempt to set the agenda for future efforts to control this ruminant pathogen. This meeting will cover all aspects of BVD virus including historic perspectives, molecular biology of the virus, pathogenesis of acute BVD virus infections as well as mucosal disease, epidemiology, diagnostics, vaccines and other control strategies. The goal of the meeting is to provide a comprehensive overview for everyone interested in the BVD virus disease complex and related Pestiviruses. The format of the meeting will consist of presentations by an international panel of invited speakers. In addition, abstracts will be solicited from those who wish to present new data from ongoing studies. These will be interspersed with the invited presentations or selected for a poster session on Monday evening. Time will be allotted for roundtable discussions to allow attendees to question and comment on the presentations. TENTATIVE SCHEDULE Sunday, June 23 5-7 PM Registration 7-10 PM Historic overview Monday June 24 8-5 PM Molecular Biology and pathogenesis Tuesday, June 25 8-5 PM Epidemiology, Diagnostics, Vaccines, Control Strategies 7-10 PM Banquet The meeting site will be the College of Veterinary Medicine with shuttle service provided from local hotels to the College. The welcoming reception on Sunday, lunches on Monday and Tuesday as well as Dinner on Monday will be provided at the conference site. Pre-registration for the meeting is required, with a registration fee of $50. Accommodations will be provided by the local hotels and motels. Reservations will need to be handled directly with the hotel of your choice. A block of 100 rooms have been set aside for the symposium at the Best Western and the Sheraton Motor Inn. Early reservations are advisable given the limited facilities in Ithaca. Ithaca is centrally isolated in upstate New York. The Ithaca airport is serviced by commuter airlines from Philadelphia and Newark and jet service from Pittsburgh. The Syracuse airport is serviced by most major airlines and is approximately 60 miles from Ithaca. Shuttle service can be arranged to Ithaca. ABSTRACTS Abstracts are being solicited from those who wish to present data from ongoing studies. The organizing committee will select those which most complement the topics addressed by the invited speakers for an oral presentation. The remaining abstracts will be assigned to the poster session. If there is a preference for the poster session please note that on your correspondence. The abstract should be restricted to 250 words. It should be clearly noted as to who will present the paper should it be selected for oral presentation. Multiple abstracts may be submitted. A decision as to the publication of the proceedings has not been made at this time. Accordingly, please submit five copies of the abstract along with a computer disc containing the submitted document. Note the software program. The deadline for submission of the abstracts in March 1, 1996. Send abstracts to: Dr. Edward J. Dubovi Diagnostic Laboratory P.O. Box 5786 Cornell University Ithaca, NY 14851-5786 **************************************************************************** From owner-diagnostics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: rscreager@aol.com (RSCreager) Newsgroups: bionet.diagnostics Subject: Re: Source of Antibodies and Antigens Date: 9 Jan 1996 01:50:40 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 6 Sender: root@newsbf02.news.aol.com Message-ID: <4ct380$i7a@newsbf02.news.aol.com> References: Reply-To: rscreager@aol.com (RSCreager) NNTP-Posting-Host: newsbf02.mail.aol.com Regarding a source of secondary antibodies - anti human IgG and anti human IgM labelled with Horseradish Peroxidase, try The Binding Site (San Diego, CA) or Kirkegaard & Perry. Both also carry anti-human IgM, but I'm not sure if its rabbbit or goat. Richard S. Creager From owner-diagnostics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!CORNELL.EDU!gaf2 From: gaf2@CORNELL.EDU (Gregory Forbes) Newsgroups: bionet.diagnostics Subject: storage of freeze dried fungal tissue Date: 9 Jan 1996 12:33:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601092031.PAA15715@postoffice4.mail.cornell.edu> NNTP-Posting-Host: net.bio.net The oomycete fungus, Phytophthora infestans, is an important plant pathogen and is routinely studied with several biochemical and molecular markers. A convenient way of processing tissue for these analyses is to lyophilize it and then grind it. I would appreciate opinions as to whether this lyophilized tissue would be best stored at room temperature or frozen. It seems to me that the apparent advantages of freezing the material could be counter balanced by the increased risk of rehydration due to condensation in the storage container (1.5 ml microfuge tubes), if the materials are thawed and refrozen several times. Thanks, Greg Greg Forbes, plant pathologist, International Potato Center (CIP) Quito, Ecuador Email: gaf2@cornell.edu CURRENTLY: Dept. Plant Pathology, Cornell University, 334 Plant Science Bldg. Ithaca, NY 14853 Fax: +1(607)2554471 Tel: +1(607)2553188 From owner-diagnostics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!BEPC2.IHEP.AC.CN!wangyq From: wangyq@BEPC2.IHEP.AC.CN Newsgroups: bionet.diagnostics Subject: food_test Date: 9 Jan 1996 08:40:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <0099C27C.E458F9E0.143@bepc2.ihep.ac.cn> NNTP-Posting-Host: net.bio.net Dear Netter: I'm planning a food testing program now. Could you please tell me something about the fast exam of food bacteria contamination? Are there dry stripes used for fluid (such as drink, milk, etc.) exam? Which technilogy are they applied, immunological or chemical? Any information would be very appreciated. Thank you. Looking forward to your reply. Best Regards, Wang Yuquan Beijing Cons Bio-Tech From owner-diagnostics@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.cais.net!news.his.com!news From: Dietmar Tietz Newsgroups: bionet.biophysics,bionet.diagnostics,bionet.genome.chromosomes Subject: --> CALL FOR PAPERS <-- Date: 9 Jan 1996 21:03:43 GMT Organization: Heller Information Services, Inc. Lines: 35 Message-ID: <4cul7f$gt3@news2.his.com> NNTP-Posting-Host: djt.his.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.12(Macintosh; I; 68K) To: djt@his.com X-URL: newsrc://news.his.com/ Xref: biosci bionet.biophysics:1572 bionet.diagnostics:528 bionet.genome.chromosomes:995 Dear Researchers: I am writing to you as the Editor of a book on Nucleic Acid Electrophoresis which is to be issued by Springer Verlag (Berlin, Heidelberg, New York), one of the leading scientific publishers. This book is intended to be part of the Springer Lab Manual Series which takes a practical approach and addresses a wide spectrum of readers extending from the college student to the senior investigator. The objective of this series is to communicate hands-on experience and to provide practical hints including easy-to-follow methods. At a time when research papers have become so short that they can barely describe the methods in sufficient detail, this lab manual will be a good opportunity to transmit to the scientific community a broader spectrum of your unpublished observations and valuable experience. Please contact me if you are interested in contributing a chapter. Our aim is to cover all aspects of electrophoresis. At this time, we are particularly interested in applications related to DNA sequencing and capillary electrophoresis. Thank you for your interest. Looking forward to hearing from you I remain, Sincerely yours, Dietmar Tietz, Ph.D. Fax: USA-(301)-681-7002 Email: djt@his.com or TVJ@CU.NIH.GOV WWW: http://www.his.com/~djt/ From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!st.rim.or.jp!tishi From: tishi@st.rim.or.jp (Tatsuzo Ishigami) Newsgroups: bionet.diagnostics Subject: Re: Wanna know magazine and websites for new diagnostic test products in U.S.A Date: 10 Jan 1996 04:08:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601101100.AA00193@tishi.st.rim.or.jp> References: <4cgocv$as9@nis.dacom.co.kr> NNTP-Posting-Host: net.bio.net On 1996/01/04 23:36:01 marcop95@bora.dacom.co.kr (Lee Sang Chan) -san wrote$B!'(J ||I'd like to know magazines and websites for New diagnostic test ||products (instrument and reagent) in U.S.A. Hello Mr. Chan: There are many magazines/newspapers on the new diagnostic test. I suggest you to subscribe the following new products news. Although those are from Europe, it covers most of US new products. 1. Clinical Laboratory International (Free) c/o Elsevier Librico N.V./PEPCO, B.P.214, B-1210 Brusseles 21, Belgium. 2. LabMedica International (Free) Globetech Publishing Corp. Reader Service Dept. P.O.Box 1, Avrupa yk. P.I.M., 34001-Istanbul, Turkey, FAX:203-762-8640 3. Clinica (440 UK-ponds) PJB Publications Ltd., 18/20 Hill Rise, Richmond, Surrey, TW10 6UA, U.K. FAX: +44-0181-332-8998 The WWW sites relating to diagnostics are as follows: 1. The Diagnostics Club: URL http://www.gemini.co.uk/biopages 2. Med TechNet, an online information service specifically designed for Clinical Laboratory Professionals. $59/year. URL: http://www.medtechnet.com I hope aboves are helpful for you. Regards, *************************************************************************** Tatsuzo Ishigami, Ph.D., Director, Strategic Business Development, SRL, Inc., Komiya-cho 51, Hachioji, Tokyo 192, Japan, Phone: +81-426-48-4036 Fax: +81-426-46-3707 E-Mail: tishi@st.rim.or.jp NiftyServe: KFG01304 CompuServe: 100156,1304 AOL: Ishigami *************************************************************************** Everything should be made as simple as possible, but not simpler!! A.E. *************************************************************************** From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in2.uu.net!news.pcix.com!root From: jlitt@capecod.net (Jerry Litt) Newsgroups: bionet.diagnostics Subject: Lack of Traffic Date: Wed, 10 Jan 1996 15:36:44 GMT Organization: a Digital Internet AlphaServer Site Lines: 7 Message-ID: <4d0bnt$nc8@alpha.pcix.com> NNTP-Posting-Host: hy41.capecod.net X-Newsreader: Forte Free Agent 1.0.82 Does anyone have an opinion as to why there is so little traffic in this group? I would have thought that there would be considerable interest but, unless I am doing something wrong, see very few postings. Should the group "charter" be changed to expand or redirect the intention? Jerry From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!user From: filter@firthcom.demon.co.uk (Steve Firth) Newsgroups: bionet.diagnostics Subject: Re: Lack of Traffic Date: Wed, 10 Jan 1996 22:18:26 +0000 Organization: Firth Consulting Lines: 30 Message-ID: References: <4d0bnt$nc8@alpha.pcix.com> Reply-To: filter@firthcom.demon.co.uk NNTP-Posting-Host: firthcom.demon.co.uk X-NNTP-Posting-Host: firthcom.demon.co.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit In article <4d0bnt$nc8@alpha.pcix.com>, jlitt@capecod.net (Jerry Litt) wrote: >Does anyone have an opinion as to why there is so little traffic in >this group? One headache I believe is that diagnostics is a commercial business, but the charter does not allow commercial discussion. Also, I suspect that companies don't want to talk about their products since it would give away competitive advantage. It is also possible that many of those in commerce simply don't have access to Usenet. I provide IT consultancy to biotech companies and my experience is that they are much poorer at providing computers and network connection to their staff than other commercial organisations. Often the priority is to give computers to the finance people, lab staff come a long way down in the pecking order. I see Kevin claims 20 posts in January, of which I received 12. Perhaps there are also some problems with the propagation of messages? I must say that my news server does seem to be having problems at the moment so I guess that the problem lies there. sci.bio.technology has been similarly quiet so it may just be the academic holidays causing the problem. ............................................................................. Firth Consulting Stephen Firth Support Services steve@firthcom.demon.co.uk CIS: 100023,3414 for Biotechnology From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!psinntp!psinntp!psinntp!psinntp!news.netvision.net.il!usenet From: hal_acup@netvision.net.il Newsgroups: bionet.diagnostics Subject: a new medical consultation site Date: Wed, 10 Jan 96 18:17:35 PDT Organization: NetVision LTD. Lines: 193 Message-ID: NNTP-Posting-Host: ts4gp7.netvision.net.il Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Hello everybody, A new interesting site in the internet: Dr.Shmuel halevi's consultation room for alternative medicine. Queries regarding health problems are submitted through a form. Dr.Halevi's speciality Chinese medicine (herbology, acupuncture etc.), dietary therapy, behavioral therapy and physiacl excercise. The address: http://wwwftp.netvision.net.il/~hal_acup/ ======================================================== From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!psinntp!psinntp!psinntp!psinntp!news.netvision.net.il!usenet From: hal_acup@netvision.net.il Newsgroups: bionet.diagnostics Subject: A medical consultation site Date: Wed, 10 Jan 96 18:07:57 PDT Organization: NetVision LTD. Lines: 117 Message-ID: NNTP-Posting-Host: ts4gp7.netvision.net.il Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Hello everybody, A new interesting site in the internet: Dr.Shmuel halevi's consultation room for alternative medicine. Queries regarding health problems are submitted through a form. Dr.Halevi's speciality Chinese medicine (herbology, acupuncture etc.), dietary therapy, behavioral therapy and physiacl excercise. The address: http://wwwftp.netvision.net.il/~hal_acup/ ======================================================== From owner-diagnostics@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: Lack of Traffic Date: 10 Jan 1996 05:38:21 -0800 Organization: SASA Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601101335.aa08606@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net Jerry Litt wrote: > Does anyone have an opinion as to why there is so little traffic in > this group? I would have thought that there would be considerable > interest but, unless I am doing something wrong, see very few > postings. Should the group "charter" be changed to expand or redirect > the intention? Recent figures for bionet.diagnostics posts are: Oct 1995 38 (a low point) Nov 75 Dec 60 Jan 1996 20(so far) This is about the same volume of posts as the bionet RAPD, HIV and human genome lists, to name three that I looked at in the archives. We certainly have a lot fewer posts than the materials and methods group which gets about 1200/month! While 60-70 posts/month isn't too bad, I would like to see more posts: a realistic target for the diagnostics list would be about 200/month, which is the volume of the virology list. I would like to encourage subscribers and readers to post abstracts of papers, notice of relevant conferences and so on and to reply to the list as a whole rather than send individual e-mail in reply to a post. Kevin (co-discussion leader) Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Wed Jan 10 22:00:00 1996 Newsgroups: bionet.diagnostics Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!in2.uu.net!News_1!not-for-mail From: carlg Subject: Journal of Clinical Chemistry Message-ID: <1b7cc$d224.3e@News_1> Date: Thu, 11 Jan 1996 18:34:04 GMT Lines: 6 Does anyone know if there is a web page for the Journal of Clinical Chemistry or some way of getting an index online? Thanks for your helpl. From owner-diagnostics@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.chatlink.com!news.erie.net!moose.erie.net!lconfer From: lconfer@moose.erie.net (Les Confer) Newsgroups: bionet.diagnostics Subject: Has anyone heard of Alkatech? Date: 11 Jan 1996 02:09:05 GMT Organization: ErieNet Lines: 7 Message-ID: <4d1rg1$hf@news.erie.net> NNTP-Posting-Host: moose.erie.net X-Newsreader: TIN [version 1.2 PL2] Has anyone heard of Alkatech Corp. in or near Research Triangle Park N.C. They supposedly have a new technology for measuring femtomole quantities of Botulism toxin using a modified ELISA procedure. Les Confer From owner-diagnostics@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!MAGI.COM!eja From: eja@MAGI.COM Newsgroups: bionet.diagnostics Subject: Re: Wanna know magazine and websites for new diagnostic test products in U.S.A Date: 10 Jan 1996 16:22:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601110020.TAA23400@infoweb.magi.com> NNTP-Posting-Host: net.bio.net In response to the following request: >On 1996/01/04 23:36:01 > marcop95@bora.dacom.co.kr (Lee Sang Chan) -san wrote$B!'(J >||I'd like to know magazines and websites for New diagnostic test >||products (instrument and reagent) in U.S.A. For Canadian sources, try: http://www.ibc.nrc.ca/ibc and: http://www.io.org/~kalyx the pages point to other sites. Hope you are successful..Eng Au. From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in1.uu.net!news.emi.com!pauling.wadsworth.org!usenet From: bobrej@wadsworth.org (Robert Rej) Newsgroups: bionet.diagnostics Subject: Re: Journal of Clinical Chemistry Date: 12 Jan 1996 13:59:37 GMT Organization: Wadsworth Center Lines: 12 Message-ID: <4d5pg9$19k@pauling.wadsworth.org> References: <1b7cc$d224.3e@News_1> NNTP-Posting-Host: alcor.wadsworth.org X-Newsreader: WinVN 0.92.6+ In article <1b7cc$d224.3e@News_1>, carlg says: > >Does anyone know if there is a web page for the Journal >of Clinical Chemistry or some way of getting an index >online? > >Thanks for your helpl. The table of contents for Clinical Chemistry is posted on the bionet.journals.contents news group and the bionet journals web pages. A direct web page is planned by the journal for sometime this year. From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: slrcmg@aol.com (SLRCmg) Newsgroups: bionet.diagnostics Subject: Re: Has anyone heard of Alkatech? Date: 12 Jan 1996 04:43:14 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 19 Sender: root@newsbf02.news.aol.com Message-ID: <4d5afi$7q4@newsbf02.news.aol.com> References: <4d1rg1$hf@news.erie.net> Reply-To: slrcmg@aol.com (SLRCmg) NNTP-Posting-Host: newsbf02.mail.aol.com Les Confer wrote: >Has anyone heard of Alkatech Corp. in or near Research Triangle Park >N.C. They supposedly have a new technology for measuring >femtomole quantities of Botulism toxin using a modified >ELISA procedure. >Les Confer You must be looking for Elcatech, Inc. Last I heard of them (about 1992-3) they were making coagulation-based immunoassays which were supposed to be very, very sensitive. I have them in Winston-Salem, NC, at 919-777-3624. Best of luck Mark From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!peer-news.britain.eu.net!strath-cs!queens-belfast.ac.uk!vsg0004 From: vsg0004@queens-belfast.ac.uk (S.MCCULLOUGH) Newsgroups: bionet.diagnostics Subject: Aujeszky's disease virus mAbs Message-ID: <1996Jan12.151723.4957@queens-belfast.ac.uk> Date: 12 Jan 96 15:17:23 GMT Organization: Queen's University of Belfast Lines: 7 I'm looking for mAbs to Aujeszky's disease (pseudorabies) virus glycoproteins, especially gE (formerly known as gI). If you can help, please reply to S.McCullough@qub.ac.uk Thanks in anticipation. From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: (Fwd) Re: Thermostable enzymes Date: 12 Jan 1996 08:03:31 -0800 Organization: SASA Lines: 55 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601121559.aa03815@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net I think that this was meant for the list, so I'm sure Geoff won't mind me re-posrting it. Kevin From Geoff Rule: I am very interested in 3SR/NASBA, though I have never tried either and have only read about them. I found your message (below) intriguing though, as I would like to learn more about these amplification techniques. Here are a few questions I have on the subject and I hope no one will mind me interjecting them. Is 3SR/NASBA generally less specific than PCR becasue of the inability to use higher temperatures? I thought I read somewhere that it is necessary to use more highly purified DNA for 3SR amplification. Is this because non-specific products are formed at the 37 C used for 3SR? Is the inability of these enzymes to work at higher temperatures then a minor or a major limitation of the 3SR/NASBA techniques when it comes to amplifying DNA/RNA from "real world" samples in a simple fashion? I would also be very interested to hear from anyone who is using either of these techniques (Kevin?) as to the ease of developing the procedure for a particular piece of DNA one is interested in. Please understand that I have done PCR only half a dozen times though I understand how it works quite thoroughly. Is it as easy to do 3SR or NASBA? Finally, I understand that 3SR and NASBA are essentially the same. Is it necessary to refer to these two acronyms together all the time or can one technique legitimately claim precedence over the other? Geoff Rule, PhD candidate >PCR and LCR are based on thermostable DNA polymerase and ligase >respectively. In the dog days between Christmas and New Year I find >myself wondering what other sequence-specific amplification methods >could be devised if suitable enzymes were available. Can anyone tell me > if there is, for example, a thermostable RNA polymerase commercially > available? It seems to me that this would greatly simplify >3SR/NASBA. > >Input/ other ideas welcomed. > >Kevin > Geoffrey S. Rule Analytical Chemistry Labs NYSAES/ Cornell University Geneva, NY 14456 1-315-787-2435 Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: Lack of Traffic Date: 12 Jan 1996 08:03:25 -0800 Organization: SASA Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601121544.aa02621@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net Steve Firsth wrote: > One headache I believe is that diagnostics is a commercial business, but > the charter does not allow commercial discussion. Yes, this is a problem - but it doesn't stem from the charter but from bionet funding. I don't think it's too big a problem though, since if there were too many 'commercial' posts there might less research-oriented ones, which is what the list was set up to cater for. I think that bionet.diagnostics and biz-biotech complement each other well in this respect. > I see Kevin claims 20 posts in January, of which I received 12. Perhaps > there are also some problems with the propagation of messages? I must say > that my news server does seem to be having problems at the moment so I > guess that the problem lies there. 29 in the archives when I looked today. If anyone wants to check their messages, the archives for all bionet groups are at http://www.bio.net/archives.html Remember too that usenet messages can take a few days to make their way into everyone's newsfeed. Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!news.nic.surfnet.nl!sun4nl!xs4all!usenet From: biopharm@xs4all.nl (dr. ing. Harold Stringer) Newsgroups: bionet.diagnostics Subject: Distributor seeks new products: microbiology and clinical chemistry Date: Fri, 12 Jan 1996 13:32:46 GMT Organization: Ridascreen - Biopharm BV Lines: 50 Message-ID: <4d5nui$a8@news.xs4all.nl> Reply-To: biopharm@xs4all.nl NNTP-Posting-Host: asd04-03.dial.xs4all.nl X-Newsreader: Forte Free Agent 1.0.82 Ridascreen-Biopharm B.V. is a rapidly growing Dutch company that operates in both the Clinical Market and in the Industrial market. We import and distribute diagnostic kits for microbiological and chemical analysis. We specialize in Industrial Diagnostics for food and feed analysis, and in Clinical Diagnostics for microbiology and clinical chemistry. Currently, we would like to expand our product ranges for both the Industrial Diagnostics and for the Clinical Diagnostics. Food and Feed analysis: * mycotoxins, antibiotics, hormones, vitamines, meat species, pesticides * bacterial toxins, yeasts and moulds, bacteria e.g. Clostridium, Vibrio Medical Microbiology: * pathogenic virusses, bacteria, and protozoa Clinical Chemistry: * vitamines, drugs and medicine abuse If your company manufactures such products, and is interested in extending sales via a succesful distributor in The Netherlands with contacts with distributors throughout the European Union, please contact us via the address below. Sincerely, Dr. Ing. H.A.R. Stringer _______________________________________________________________________ Work: Private: Ridascreen-Biopharm BV Harold Stringer Dept. Clinical Diagnostics Jimi Hendrixstraat 42 dr.ing. H.A.R. Stringer 1311 HZ Almere Stationsplein 40 The Netherlands 1315 KT Almere Tel/Fax +31 - (0)36 53 64 798 The Netherlands Tel +31 - (0)36 53 43 445 Fax +31 - (0)36 53 47 265 E-mail biopharm@xs4all.nl (C) dr. ing. H.A.R. Stringer, 1996 _______________________________________________________________________ From owner-diagnostics@net.bio.net Fri Jan 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!gossip.pyramid.com!news.sedona.net!client19.sedona.net!user From: SMI@sedona.net (Linda St. James) Newsgroups: bionet.diagnostics Subject: Job Open: Surface Immob. of Biologics to Silicon Date: Fri, 12 Jan 1996 11:05:18 -0700 Organization: Search Masters International Lines: 26 Message-ID: NNTP-Posting-Host: client19.sedona.net My company has been retained by a client company developing new medical devices, to find a Senior Scientist or Associate Scientist, with a background of surface chemistry and specifically the immobilization of biological molecules on silicon surfaces. Anyone who knows "where to find" such chemists will surely earn a big pat on the back from our search firm (we have a long memory for favors). The work is fascinating, and involves the cutting edge between surface chemistry, electronics, and biotechnology. This person will be one of the major players at this young company (with significant stock options) and will be working with the modification of surfaces to attach antibodies. My client company is in the Southwest USA and is a leader in the field. Your resume or email correspondence should be addressed to: Dave Jensen Search Masters International Five Hundred Foothills South, Suite #2 Sedona, AZ 86336 (520) 282-3553 Phone or (520) 282-5881 Fax Hard Copies Please - Standard Industry Practice: Faxes not Archived http://smi.bio.com/ * See SMI profiled in "Hot Small Business" Issue of Entrepreneur Mag., 12/95 * From owner-diagnostics@net.bio.net Fri Jan 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: CHESNEAU Véronique <100710.1476@CompuServe.COM> Newsgroups: bionet.diagnostics Subject: Itiris Date: 13 Jan 1996 11:33:04 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 2 Message-ID: <4d859g$37a$1@mhafn.production.compuserve.com> Can someone tell me more about this quite mysterious disease? It's difficult to obtain information about it From owner-diagnostics@net.bio.net Fri Jan 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!news.dfn.de!uni-muenster.de!news From: Torsten Boerchers Newsgroups: bionet.diagnostics Subject: PCR with food Date: 13 Jan 1996 14:33:56 GMT Organization: ICB Lines: 27 Message-ID: <4d8fsk$gic@majestix.uni-muenster.de> NNTP-Posting-Host: arnheim.uni-muenster.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Hi, I am aware that this group is probably mostly devoted to clinical diagostics but perhaps some may know whether PCR is frequently used in species identification (not virus or bacteria, but e.g. for detection of fraudulently labelled meat etc.). Any commerical kits for this? Since MEDLINE is probably not indexing some relevant Journals for this topic, do you have any suggestion for a source that I should monitor? Sorry if this is not the appropriate news-group. Is there one which is more appropriate? Many thanks for any help Torsten Boerchers ------------------------------------------------------------------------/ Torsten Boerchers / _/ _/_/_/ _/_/_/ Institute of Chemical- and Biochemical Sensor/ _/ _/ _/ _/ Research (Molecular Biology Group) / _/ _/ _/_/_/ Mendelstr 7, D-48149 Muenster, Germany / _/ _/ _/ _/ Fax: +49-251-980 2890 Phone: +49-251-980 2880/ _/ _/_/_/ _/_/_/_/ E-Mail: borcher@uni-muenster.de / -----------------------------------------------------------------/ From owner-diagnostics@net.bio.net Fri Jan 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!gatech!newsfeed.internetmci.com!uwm.edu!newsspool.doit.wisc.edu!decwrl!tribune.usask.ca!canopus.cc.umanitoba.ca!pooh.freenet.mb.ca!winnie.freenet.mb.ca!gip204 From: Richard Drummond Newsgroups: bionet.diagnostics Subject: RSV testing Date: Sat, 13 Jan 1996 11:33:08 -0600 Organization: Blue Sky FreeNet of Manitoba Inc. Lines: 9 Message-ID: NNTP-Posting-Host: winnie.freenet.mb.ca Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Since there seems to be so little traffic on this news group right now I was wondering if everyone doing testing for RSV by a elisa is satisfied with their results? Has anyone looked at their culture results and compared them to their elisa results? Richard gip204@freenet.mb.ca From owner-diagnostics@net.bio.net Sat Jan 13 22:00:00 1996 Path: biosci!agate!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: Bert Gold Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal,sci.med Subject: COST BENEFIT DECISION ANALYSIS FOR AMNIOCENTESIS Date: Sat, 13 Jan 1996 18:29:23 -0800 Organization: UCSF, ITS Lines: 54 Message-ID: NNTP-Posting-Host: itsa.ucsf.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Xref: biosci bionet.diagnostics:548 bionet.diagnostics.prenatal:99 sci.med:106072 Clinicians interested understanding and applying psychosocial AND medical economic considerations to the current high level of amniocentesis being carried out on the basis of Advanced Maternal Age, may be interested in the following abstract: (I found these ideas fascinating) Bert Gold,Ph.D. UCSF, Program in Medical Genetics DECISION ANALYSIS OF AMNIOCENTESIS FOR PRENATAL DIAGNOSIS Y Shahar, JW Egar and R Pichumani Section on Medical Informatics, Stanford University School of Medicine, Stanford, CA. We present two models that guide clinicians and parents who must decide whether to perform amniocentesis (AC) for detection of fetal abnormalities: a personal utility model for parents, and a cost benefit model for a health-care provider. These models can tailor general guidelines to specific AC decisions. The American College of Obstetricians and Gynecologists (ACOG) provides separate guidelines, mainly concerning the use of AC and of high-resolution ultrasound (HRUS), for detection of Down's syndrome (DS) and neural-tube defects (NTD's). We used influence diagrams to model two decisions: (1) the parent's decision, using preference probabilities as personal utility measures; and (2) the decision of a policy-making organization, such as a large health-care organization, using monetary utility measures for test costs and for the values of life and disability. In both cases, we tailor the model to the particular case by using parent-specific prior probabilities for DS and NTD, as well as the probability for miscarriage due to the AC procedure The results of the analysis indicate significant sensitivity of the AC decision to parent specific utilities. Such utilities have been elicited by researchers such as Pauker and colleagues, and have been shown to vary widely. The AC decision also is sensitive to the rate of miscarriage caused by the AC procedure, but is sensitive to the DS prior probability (e.g., by age) only for certain personal-utility values. The NTD's prior probability plays a significant role mainly in cases where the decision is otherwise not obvious. In the case of the model using monetary measures, break-even points for the health-care provider can be shown for all three case-specific prior probabilities, given the value of life and the cost of lifelong disability. The AC decision is affected by parent-specific personal utilities and prior probabilities for DS and NTD's, and by local rates of miscarriage due to AC. Monetary utility measures can be used in the case of a large-scale policy. Both measures of utility might be combined, maximizing societal and individual utilities. Using only arbitrary cutoff values (e.g. age), considering ouly one disorder at a time, or including only one test procedure oversimplifies the AC decision. Additional nodes, signifying relevant parameters and utilities, can be added with relative ease to both of our influence-diagram models. We demonstrate the need for both comprehensive data and parent-specific utilities by a four-way sensitivity analysis and several exemplary cases. Discussion of this issue by netters is invited. From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!news.pcix.com!root From: jlitt@capecod.net (Jerry Litt) Newsgroups: bionet.diagnostics Subject: Traffic Date: Mon, 15 Jan 1996 16:36:59 GMT Organization: Plymouth Commercial Internet eXchange Lines: 8 Message-ID: <4ddl4m$r5m@alpha.pcix.com> NNTP-Posting-Host: hy31.capecod.net X-Newsreader: Forte Free Agent 1.0.82 Does anyone have an opinion as to why there is so little traffic in this group? I would have thought that there would be considerable interest but, unless I am doing something wrong, see very few postings. Should the group "charter" be changed to expand or redirect the intention? Jerry From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: PCR with food Date: 15 Jan 1996 03:54:06 -0800 Organization: SASA Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601151151.aa24308@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net > I am aware that this group is probably mostly devoted > to clinical diagostics but perhaps some may know whether > PCR is frequently used in species identification (not > virus or bacteria, but e.g. for detection of fraudulently > labelled meat etc.). Any commerical kits for this? > PCR is definitely used for this purpose because I know of one project where someone was using molecular marker methods to distinguish the origin of marijuana. I should point out that this was for police forensic purposes rather than any sort of quality control scheme of the drug dealers.:-) I don't know of any commercial kits though. > Since MEDLINE is probably not indexing some relevant > Journals for this topic, do you have any suggestion for > a source that I should monitor? > The sort of journals you might want to look at are Journal of Food Science and Technology, Food Science etc. Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!usp.br!lpereira From: lpereira@usp.br (Lygia da Veiga Pereira) Newsgroups: bionet.diagnostics Subject: help w/ PCR leptospirose Date: 15 Jan 1996 09:30:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I need information on any protocol or diagnostic kit to identify leptospiroses by PCR. Thank you, Lygia V. Pereira, Ph.D. From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!OP1.UP.AC.ZA!KPAT1 From: KPAT1@OP1.UP.AC.ZA ("Fred Reyers") Newsgroups: bionet.diagnostics Subject: Re: Traffic Date: 15 Jan 1996 06:26:13 -0800 Organization: University of Pretoria Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <13806906597@op1.up.ac.za> NNTP-Posting-Host: net.bio.net Jerry asked the question about traffic. > To: diagnost@net.bio.net > From: jlitt@capecod.net (Jerry Litt) > Subject: Traffic > Date sent: Mon, 15 Jan 1996 16:36:59 GMT > Does anyone have an opinion as to why there is so little traffic in > this group? Jerry, I think one of the possible reasons COULD be that the "automatic" reply facility on messages received from this list (using my Pegassus Mail) does not, by default, also send a copy of the reply to the diagnost-list. Most of the other lists that I subscribe to do do this and consequently I do not have to type ".. , diagnost@net.bio.net .." behind the name of the originator of the mail to which I wish to reply in order to "involve" others. Regards Fred Fred Reyers (Prof & Head: Section of Clinical Pathology) Department of Medicine Faculty of Veterinary Science P.Bag X04, ONDERSTEPOORT, 0110, Rep. of South Africa Tel: 27-12-529 8288 FAX: 27-12-529 8308 or 27-12-344 2107 (Home) "The great tradgedies of science are the slaying of beautiful hypotheses by ugly facts" (Huxley) From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!OP1.UP.AC.ZA!KPAT1 From: KPAT1@OP1.UP.AC.ZA ("Fred Reyers") Newsgroups: bionet.diagnostics Subject: Re: PCR with food Date: 15 Jan 1996 06:15:25 -0800 Organization: University of Pretoria Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <137DA3C05D3@op1.up.ac.za> NNTP-Posting-Host: net.bio.net Hi Torsten Boerchers wrote on 13 Jan 1996 > I am aware that this group is probably mostly devoted > to clinical diagostics but perhaps some may know whether > PCR is frequently used in species identification (not > virus or bacteria, but e.g. for detection of fraudulently > labelled meat etc.). Any commerical kits for this? I am not aware of commercial "kits" but do know that our police forensic people are developing PCR's for these purposes. Another group of workers that may be worth approaching are those identifying host species from gut "bloodmeal" contents in Trypanosomiasis, Leishmaniasis and Malaria research. Regards Fred Fred Reyers (Prof & Head: Section of Clinical Pathology) Department of Medicine Faculty of Veterinary Science P.Bag X04, ONDERSTEPOORT, 0110, Rep. of South Africa Tel: 27-12-529 8288 FAX: 27-12-529 8308 or 27-12-344 2107 (Home) "The great tradgedies of science are the slaying of beautiful hypotheses by ugly facts" (Huxley) From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!MAILHOST.CAPECOD.NET!jlittcapecod.net From: jlittcapecod.net@MAILHOST.CAPECOD.NET (Jerry Litt) Newsgroups: bionet.diagnostics Subject: Re: Traffic Date: 15 Jan 1996 09:33:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601151733.MAA03315@mailhost.capecod.net> NNTP-Posting-Host: net.bio.net Thanks for the response, Fred. I had a message that 39 postings had come in since the beginning of January. I have not seen nearly this many so perhaps the Free Agent software that I use has similar characteristics to the system you use. Jerry At 04:26 PM 1/15/96 GMT+2, Fred Reyers wrote: >Jerry asked the question about traffic. > >> To: diagnost@net.bio.net >> From: jlitt@capecod.net (Jerry Litt) >> Subject: Traffic >> Date sent: Mon, 15 Jan 1996 16:36:59 GMT > >> Does anyone have an opinion as to why there is so little traffic in >> this group? > >Jerry, > >I think one of the possible reasons COULD be that the "automatic" >reply facility on messages received from this list (using my Pegassus >Mail) does not, by default, also send a copy of the reply to the >diagnost-list. Most of the other lists that I subscribe to do do this >and consequently I do not have to type ".. , diagnost@net.bio.net .." >behind the name of the originator of the mail to which I wish to >reply in order to "involve" others. > >Regards > >Fred >Fred Reyers (Prof & Head: Section of Clinical Pathology) >Department of Medicine >Faculty of Veterinary Science >P.Bag X04, ONDERSTEPOORT, 0110, >Rep. of South Africa >Tel: 27-12-529 8288 >FAX: 27-12-529 8308 or 27-12-344 2107 (Home) > > "The great tradgedies of science > are the slaying of beautiful hypotheses by ugly facts" > (Huxley) > > From owner-diagnostics@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!idbiomed.com!FouziB From: FouziB@idbiomed.com (Fouzi Bekkaoui) Newsgroups: bionet.diagnostics Subject: Ribosomal protein Date: 15 Jan 1996 09:42:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <30FA917B@mailbox.electric.net> NNTP-Posting-Host: net.bio.net I would like to know if anybody knows of a commercial source of ribosomal proteins ? Thank you. ~~~~~~~~~~~~~~~~~~~~~~~~~~ Fouzi Bekkaoui Phone (604) 431 9314 Fax (604) 431 9378 Email fouzib@idbiomed.com ~~~~~~~~~~~~~~~~~~~~~~~~~~ From owner-diagnostics@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.chatlink.com!news.erie.net!news From: lconfer@moose.erie.net (Leslie Confer) Newsgroups: bionet.diagnostics Subject: Looking for antibodies to IL-6 and Lysozyme Date: Wed, 17 Jan 96 05:10:23 GMT Organization: ErieNet Lines: 4 Message-ID: <4di0bv$ev8_002@news.erie.net> NNTP-Posting-Host: boris.erie.net X-Newsreader: News Xpress Version 1.0 Beta #4 I'm looking to evaluate alternative sources of antibodies to interleukin 6 and human lysozyme (muramidase). I will consider both polyclonal and monoclonal from various species. I would appreciate any leads. From owner-diagnostics@net.bio.net Wed Jan 17 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!news.funet.fi!mordred.cc.jyu.fi!news From: Matti Vuento Newsgroups: bionet.diagnostics Subject: Clamydial peptidase Date: 18 Jan 1996 08:48:33 GMT Organization: University of Jyvaskyla, Finland Lines: 10 Message-ID: <4dl1h1$i87@mordred.cc.jyu.fi> NNTP-Posting-Host: vap210a.pcbio.jyu.fi Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) There is a commercial diagnostic test for Chlamydia trachomatis which they claim to detect a chlamydia-specific peptidase. I have so far found no published data to support this claim. Does anyone have information on chlamydial peptidase? I would greatly appreciate any information. Matti Vuento Biochemistry/ University of Jyvaskyla, Finland From owner-diagnostics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!EU.net!peer-news.britain.eu.net!psinntp!psinntp!psinntp!psinntp!interramp.com!usenet From: "Kenneth Bromberg, MD" Newsgroups: bionet.diagnostics Subject: Re: RSV testing Date: Fri, 19 Jan 1996 01:18:08 -0500 Organization: SUNY Health Science Center @ Brooklyn -Vaccine Center Lines: 34 Message-ID: <30FF37A0.50B3@interramp.com> References: NNTP-Posting-Host: ip165.new-york2.ny.interramp.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b5 (Win95; I) Richard Drummond wrote: > > Since there seems to be so little traffic on this news group right now > I was wondering if everyone doing testing for RSV by a elisa is satisfied > with their results? Has anyone looked at their culture results and compared > them to their elisa results? > > Richard > > gip204@freenet.mb.caAn informal discussion with my local virology laboratory suggests sensitivities below 90% this year. I was involved with most of the original RSV kits and their field trials. The sensitivity since thse trials (90-95%) seems to have decreased but this is really difficult to quantify. There is so much variability with culture (look in the literature and you will see differing sensitivity each year for RHMK vs. HEp-2). A panel of specimens, cultured at one time and then frozen in alequots might answer these questions as could a mixture of recombinant antigens. I think the Testpack is the best test. I've seen problems the Directogen (did I spell that wrong) in the low range. Remember..Most DFA and EIA results during RSV season are true positives and that cell culture is not the gold standard. I would suggest a combination of culture, antigen detection with a blocking test (or multiple antigen detection test during the evaluation of several kits) or DFA seen by two independent observers as true positives. We published some data in AM J Clin Path about using blind DFA of cells to find RSV that did not produce CPE because of overgrowth (? adeno) or other factors. For small numbers of samples I still like DFA. The Journal of Clinical Micro is full of comparisons about these tests. PCR is OK for it as well but I would consider it overkill. Happy wheezing. Ken Bromberg, MD Pediatric ID and microbiology for the fun of it. From owner-diagnostics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.wco.com!news From: Della Noche Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal,sci.med,alt.com Subject: Re: COST BENEFIT DECISION ANALYSIS FOR AMNIOCENTESIS Date: 19 Jan 1996 09:41:26 GMT Organization: Ca. Flood Predicted for 1/22-25 in Farmer's Almanac and Look at That Rain! Lines: 14 Message-ID: <4dnp06$14l@news.wco.com> References: NNTP-Posting-Host: venus51.calon.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) Xref: biosci bionet.diagnostics:564 bionet.diagnostics.prenatal:102 sci.med:106762 I have long wondered if amniocentesis demanded by insurers was paying off for them and have suspected it isn't. Still, since they still seem to need it... I'd really be interested in seeing some numbers. The abstract implies, esp. with its mention of "break-even" points for the 3 bases of decision that these figures are available. Where are they? Love to see them! Thanks! DN From owner-diagnostics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!SNUFFY.FHHOSP.AB.CA!GIBB From: GIBB@SNUFFY.FHHOSP.AB.CA Newsgroups: bionet.diagnostics Subject: RE: RSV testing: PCR Overkill? Date: 19 Jan 1996 11:54:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <960119125157.15d59@Snuffy.FhHosp.Ab.Ca> Reply-To: Paddy.Gibb@CRHA-Health.Ab.Ca NNTP-Posting-Host: net.bio.net Kevin O'Donnel asks: > Are there many cases where PCR is not overkill? Or to put the > question another way, aside from screening for mutations, for which > applications in the clinical or plant pathology fields is PCR now the > method of choice? This is a very good question. Here is my personal answer - and I'd be most interested in any other views. There does seem to be a feeling in some quarters that PCR will replace all of current diagnostic microbiology. That could be true, but I do remember similar promises being made for antigen detection kits about 10 years ago. They were going to convert all of microbiology into bedside tests - it was just a matter of making the right monoclonals. Another impresion I come across is that any lab which cannot do PCR at the drop of a hat must be living in the dark ages. In my opinion there are just being cautious, and not jumping on the bandwagon. PCR does have a place now for diagnosis and management of hepatitis C. It probably has a place in diagnosis of TB, though it is not yet clear quite where. It is not sensitive enough to screen out negative specimen prior to culture, and does not (yet) give sensitivity results. It may be used as a quick test to confirm that a smear-positive specimen does indeed contain Mycobacterium tuberculosis, but that is an expensive approach and not really much faster than "conventional" Bactec culture followed by probe-based species identification. It is useful in pathology for dealing with fixed tissue. There are some other specific organisms that are hard to recover by conventional methods, and may be detected better by PCR, but I don't think that PCR is established as the best diagnostic method for any of these. In many cases these organisms are not common, or not commonly looked for. There are some "high-volume" examples where PCR (and other amplification methods) are being investigated annd promoted by large companies to replace current technologies. Chlamydia and gonococcus testing come to mind. I don't think that amplification technology offers a huge advantage in these cases, though there are some benefits. (The biggest benefit will perhaps be to the company that comes out with a really good system - or is that being too cynical?) Benefits include the ability to test urine rather than cervical swabs for Chlamydia, for example. There are some disadvantages to PCR over culture for any of these organisms. Lack of antibiotic sensitivity tests is one, and lack of a whole genome for PFGE etc is another. These are potentially soluble problems. The basic problems of false-positive results has not yet been answered however. The automated systems may well get round it, but for any "home-made" PCR system there is a very real potential problem. There is a very good paper on this point in J Clin Micro (Feb 1994, vol 32, p 277-84) showing that some apparently well-organised labs had large numbers of false positives in doing PCR for TB. No diagnostic microbiology lab which hopes to survive can afford to ignore PCR etc. We need to study it, and we need to get some expertise. But I don't think we need feel obliged to actually do much of it right now, with the exception of Hep C. Paddy Gibb MB, PhD Foothills Hospital & Provincial Public Health Laboratory Calgary. From owner-diagnostics@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Dr Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Re: RSV testing Date: 19 Jan 1996 03:43:19 -0800 Organization: Scottish Agricultural Science Agency Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601191139.aa19779@jura.sasa.gov.uk> NNTP-Posting-Host: net.bio.net On 19 Jan 96 at 1:18, Kenneth Bromberg, MD wrote: > The Journal of Clinical Micro is full of comparisons about these > tests. PCR is OK for it as well but I would consider it overkill. Are there many cases where PCR is not overkill? Or to put the question another way, aside from screening for mutations, for which applications in the clinical or plant pathology fields is PCR now the method of choice? Kevin Dr Kevin O'Donnell "Work as if you were in the early days Diagnostics and Molecular Biology of a better nation" SASA - Alasdair Gray Edinburgh From owner-diagnostics@net.bio.net Fri Jan 19 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!torn!nott!bcarh189.bnr.ca!bcarh8ac.bnr.ca!bmdhh222.bnr.ca!aksingh From: aksingh@bnr.ca (Arvind Singh ) Newsgroups: bionet.diagnostics Subject: Help S.L.E. (lupus) patient Date: 20 Jan 1996 09:42:50 GMT Organization: Bell Northern Research Lines: 23 Message-ID: <4dqdeq$evf@bmdhh222.bnr.ca> NNTP-Posting-Host: bmdhh179.bnr.ca X-Newsreader: TIN [version 1.2 PL2] Subject: please pass on this message to friends, urgent| Dear friend, I'm Rupam, Srirang's friend from IIMB. My sister is an S.L.E. (lupus) patient. if you know any person having any information about the latest developments regarding the disease and slowing it's progress, please contact at the following address :- grp2@alpha2.iimb.ernet.in Postal address: Rupam Prasad, A-1st Pantry, PGP Hostel, Indian Institute of Management, Bannerghatta Road, Bangalore-560076. India Please pass on this message to as many people as you can. Any information will be highly appreciated. thanks a lot, Rupam. From owner-diagnostics@net.bio.net Fri Jan 19 22:00:00 1996 Path: biosci!UVA.PCMAIL.VIRGINIA.EDU!mgk2r From: mgk2r@UVA.PCMAIL.VIRGINIA.EDU ("Michael G. Kurilla") Newsgroups: bionet.diagnostics Subject: RE: RSV testing: PCR Overkill? Date: 20 Jan 1996 14:54:50 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 162 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601202252.RAA05420@uva.pcmail.Virginia.EDU> NNTP-Posting-Host: net.bio.net On Jan 20, 3:39am, GIBB@snuffy.fhhosp.ab.ca wrote: > Subject: RE: RSV testing: PCR Overkill? > Kevin O'Donnel asks: > > > Are there many cases where PCR is not overkill? Or to put the > > question another way, aside from screening for mutations, for which > > applications in the clinical or plant pathology fields is PCR now the > > method of choice? > > This is a very good question. Here is my personal answer - and I'd be most > interested in any other views. Let me add some views as a clinical microbiologist and someone who does PCR. > > There does seem to be a feeling in some quarters that PCR will replace all of > current diagnostic microbiology. That could be true, but I do remember similar > promises being made for antigen detection kits about 10 years ago. They were > going to convert all of microbiology into bedside tests - it was just a matter > of making the right monoclonals. One advantage of PCR over antigen detection is that once specimen preparation is optimized then the procedure becomes organism independent. That is, any primer pair will have some temperature optimum and it will work. With antibodies every new antibody has its own quirks (some can immunoprecipitate, some Western, some can be used in fluorescence, etc.). Primers pairs can also be made by anyone once the sequence is known. > > Another impresion I come across is that any lab which cannot do PCR at the drop > of a hat must be living in the dark ages. In my opinion there are just being > cautious, and not jumping on the bandwagon. There is certainly a degree of prestige with doing the latest hi-tech thing, but, I would venture that more problems crop up with underexperienced labs attempting procedures beyond their technical skill. Everyone doesn't need to do it and in terms of allocation of health care dollars, everyone shouldn't. > > PCR does have a place now for diagnosis and management of hepatitis C. > > It probably has a place in diagnosis of TB, though it is not yet clear quite > where. It is not sensitive enough to screen out negative specimen prior to > culture, and does not (yet) give sensitivity results. It may be used as a > quick test to confirm that a smear-positive specimen does indeed contain > Mycobacterium tuberculosis, but that is an expensive approach and not really > much faster than "conventional" Bactec culture followed by probe-based species > identification. Take a look at some of the non-PCR nucleic based procedures. SDA is starting to look good (granted, not much clinical data yet) and could provide good clinical info. This issue is complicated because we have a tendency to evaluate clinical tests by how they improve the lab's productivity (cheaper test, faster test, simpler test, etc.). It will take longer (perhaps not much with pressure from hospitals themselves) to evalaute the overall savings in total health care dollars from faster lab diagnoses (I'm thinking about TB verses non TB mycobacterial infections, for example). > It is useful in pathology for dealing with fixed tissue. This is still virgin area to be tapped. > > There are some other specific organisms that are hard to recover by > conventional methods, and may be detected better by PCR, but I don't think that > PCR is established as the best diagnostic method for any of these. Until PCR kits are common, it is unlikely any will be established as "best." The current "homebrew" nature of PCR means that average success rate is held back by alot of inferior procedures. > In many > cases these organisms are not common, or not commonly looked for. True, but in many instances those organisms can be the cause of alot of health dollars in total. In addition, antigen detection kits may not be cost effective because of a limited shelf life and infrequent use. In addition, I think with multiple PCR procedures in place, the technical skill of the lab will be better maintained and easier to maintain. > > There are some "high-volume" examples where PCR (and other amplification > methods) are being investigated annd promoted by large companies to replace > current technologies. Chlamydia and gonococcus testing come to mind. I don't > think that amplification technology offers a huge advantage in these cases, > though there are some benefits. (The biggest benefit will perhaps be to the > company that comes out with a really good system - or is that being too > cynical?) Benefits include the ability to test urine rather than cervical > swabs for Chlamydia, for example. Testing urine also means that less expense is generated in sample procurement and opens the possibility of more frequent testing, particularly in non hi-tech sites. > > There are some disadvantages to PCR over culture for any of these organisms. > Lack of antibiotic sensitivity tests is one, and lack of a whole genome for > PFGE etc is another. Some sensitivity issues may be resolvable by PCR (+/- for resistance gene), but certainly not all. > These are potentially soluble problems. The basic > problems of false-positive results has not yet been answered however. The > automated systems may well get round it, but for any "home-made" PCR system > there is a very real potential problem. There is a very good paper on this > point in J Clin Micro (Feb 1994, vol 32, p 277-84) showing that some apparently > well-organised labs had large numbers of false positives in doing PCR for TB. > Standardized kits will go a long way to helping this. In addition, we are still on the steep part of the learning curve. > No diagnostic microbiology lab which hopes to survive can afford to ignore PCR > etc. We need to study it, and we need to get some expertise. But I don't > think we need feel obliged to actually do much of it right now, with the > exception of Hep C. > We have pressure for other tests from clinicians. CSF Toxo in AIDS patients is one. CSF HSV testing looks pretty good as well. Here at UVa, we also do Bordatella PCR instead of DFA for confirmation (I know, not common). Beyond PCR for diagnostic purposes, there are other issues. One is that I think there are applications for DNA based assessments for management in addition to diagnosis (quantitation of Hep C, for example). I also firmly believe (and I'm sure many will take issue with me), that at some point in the future, taxomony will be based on nucleic acid sequence and not arbitrary biochemical reactions. Perhaps one of the better outcomes of the genome sequencing project, will be the development of rapid, self contained, and most importantly, cheap, automated sequencers. I expect long before I am retired, species identification will be based on ribosomal RNA gene sequences. Mike Kurilla From owner-diagnostics@net.bio.net Sun Jan 21 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!info.ucla.edu!library.ucla.edu!csulb.edu!lgeller From: lgeller@csulb.edu (Louis Geller) Newsgroups: bionet.diagnostics Subject: Need Help!!! Date: 22 Jan 1996 03:05:19 GMT Organization: Cal State Long Beach Lines: 26 Message-ID: <4duutf$e1c@garuda.csulb.edu> NNTP-Posting-Host: jaeger.csulb.edu X-Newsreader: TIN [version 1.2 PL2] A few months ago, my fiancee, Eileen, died from a very serious blood clot - superior sagittal sinus thrombosis. She was 24 years old. This is really causing me a tremendous amount of grief and pain. It is unbelievable. I am interested in finding out about CAT scans; specifically the differences between infused and non-infused scans. What, exactly, does the IV contrast medium allow a doctor to see? What diagnostic benefits does the +/- contrast offer, compared to only the non-infused? Why would a doctor only do a non-infused CT scan when trying to figure out why a young woman was having excruciating headaches, with no prior history of migraines? Also, what will an M.R.I. show? I am interested in finding out as much as possible about this. For my fiancee, only a non-infused CT scan was done. No MRI was performed, and I would like to find out the reasoning. Seems kind of late, though. I am a graduate student in cell/molecular biology. As I said, this has really messed my life up, and I would like some help. Please send me any thoughts by reply mail. Thank you very much. Louis Geller, Devastated Fiancee lgeller@csulb.edu -- From owner-diagnostics@net.bio.net Sun Jan 21 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!news00.sunet.se!sunic!news99.sunet.se!erinews.ericsson.se!nrk0140.ericsson.se!ETXMIWE From: ETXMIWE@helix.ericsson.se (Mikael Wennström) Newsgroups: bionet.diagnostics Subject: klippel trennanay Date: Tue, 23 Jan 1996 00:36:21 Organization: Ericsson Telecom AB Lines: 4 Message-ID: NNTP-Posting-Host: nrk0140.ericsson.se X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I wonder if somebody could help me to find out what my son which have been diagnostic klippel trennany what sikness is that.? sorry about my bad english. From owner-diagnostics@net.bio.net Mon Jan 22 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!waikato!rpc253 From: COLLINR@Agresearch.cri.nz (ROGER COLLIN) Newsgroups: bionet.diagnostics Subject: ELISA blues - thanks Date: Tue, 23 Jan 96 04:51:40 GMT Organization: AgResearch Ruakura Lines: 16 Message-ID: <4e1lp8$1j16@thebes.waikato.ac.nz> NNTP-Posting-Host: 160.4.128.85 X-Newsreader: News Xpress Version 1.0 Beta #4 Many thanks to all the people who emailed me suggestions to my problems on edge effects and storage of ELISA plates. I got over 30 responses! A common theme was the idea of freezing plates after coating and blocking rather than trying to dry the plate. I tried this and it has worked fine! My standard curves are nearly identical on feshly prepared plates and on plates that have been frozen after blocking. I can say after quite a few attempts that there are some things to avoid; drying plates, which in my hands decreases the max OD by about 1/4 and increases interwell %cv's, and stacking plates which I suspect was what was giving me my edge effects. I was stacking my plates 5-10 high. Anyhow thanks again - it was great to see how a problem can be solved with experts all around the world contributing. Roger Collin AgResearch New Zealand From owner-diagnostics@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!psinntp!psinntp!psinntp!psinntp!interramp.com!usenet From: "Kenneth Bromberg, MD" Newsgroups: bionet.diagnostics Subject: Re: RSV testing Date: Tue, 23 Jan 1996 23:30:05 -0500 Organization: SUNY Health Science Center @ Brooklyn -Vaccine Center Lines: 28 Message-ID: <3105B5CD.6D3@interramp.com> References: <9601191139.aa19779@jura.sasa.gov.uk> NNTP-Posting-Host: 38.13.98.104 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b5 (Win95; I) To: Dr Kevin O'Donnell Dr Kevin O'Donnell wrote: > > On 19 Jan 96 at 1:18, Kenneth Bromberg, MD wrote: > > > The Journal of Clinical Micro is full of comparisons about these > > tests. PCR is OK for it as well but I would consider it overkill. > > Are there many cases where PCR is not overkill? Or to put the > question another way, aside from screening for mutations, for which > applications in the clinical or plant pathology fields is PCR now the > method of choice? > > Kevin > > Dr Kevin O'Donnell "Work as if you were in the early days > Diagnostics and Molecular Biology of a better nation" > SASA - Alasdair Gray > EdinburghFor B. pertussis diagnosis, PCR (with all of the usual limitations) is probably the method of choice. We are working on our data for DFA and PCR but all of them are much better than culture which is very specific but not that sensitive. Another area would be bugs that are hard to grow like Bartonella or H. ducreyi. TB also ...I know that there was a comparison in the JCM that suggested a dart board might be a better test but we await methods to control contamination. Ken Bromberg, MD Peds ID, Kings County Hospital Everyone was born in Brooklyn From owner-diagnostics@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!biosci!not-for-mail From: nberman@ucla.edu (Nancy Berman) Newsgroups: bionet.agroforestry,bionet.audiology,bionet.biophysics,bionet.cellbiol,bionet.diagnostics,bionet.general,bionet.immunology,bionet.microbiology Subject: Survey of women in science Date: 24 Jan 1996 02:47:16 -0800 Organization: UCLA Lines: 24 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Keywords: Women, Science, Engineering Xref: biosci bionet.agroforestry:2541 bionet.audiology:1131 bionet.biophysics:1624 bionet.cellbiol:3846 bionet.diagnostics:574 bionet.general:19525 bionet.immunology:7313 bionet.microbiology:4672 We are interested in doing a survey to document the experience of women who finished their undergraduate work in the fifties, and went on to get an advanced degree in Engineering, Sciences or Mathematics. We are particularly interested in women who postponed or dropped their career plans in favor of marriage and motherhood then continued their education at some later point in the same or a different field. We would like to ask you to complete a short questionnaire. The information will be used only for research and will be kept completely confidential. There is no comeercial purpose behind this request. If you are interested in participating please respond to either of us. Please indicate your preferred method of receiving mail, US Postal, FAX, E-mail (we can't afford Federal Express). If you know of another news group, e-mail list or other women that should receive this message, please show them this article or let us know how to reach them. Thank you for replying. We apologize for any cross-posting. Roberta S. Madison, Dr. P.H., Nancy G. Berman, Ph.d Professor of Epidemiology Assistant Professor of Pediatrics Department of Health Sciences Department of Pediatrics California State University at Harbor-UCLA Medical Center Northridge 1000 W Carson Street Northridge, CA 91330-8485 Torrance, CA 90509 FAX: (818)-885-2045 FAX: (310) 476-4582 E-mail: Rmadison@huey.csun.edu E-mail: Nberman@UCLA.EDU From owner-diagnostics@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!rutgers!gatech!newsxfer2.itd.umich.edu!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!tube.news.pipex.net!pipex!dish.news.pipex.net!pipex!gwen.pcug.co.uk!kate.ibmpcug.co.uk!imagen!dakodl Newsgroups: bionet.diagnostics Message-ID: <108@imagen.win-uk.net> Reply-To: dakodl@imagen.win-uk.net (DAKO Diagnostics Ltd) From: dakodl@imagen.win-uk.net (DAKO Diagnostics Ltd) Date: Wed, 24 Jan 1996 15:02:24 GMT Subject: Job Available - Technical Support Scientist Lines: 40 DAKO Diagnostics Ltd makes immunodiagnostic kits and products for hospital microbiology and clinical chemistry laboratories worldwide. We now wish to recruit a Technical Support Scientist, to work in our Technical Services Department. This is a laboratory based position which will involve all aspects of planning and running validation studies. The job will also involve product support and troubleshooting. Previous experience of immunoassay techniques, diagnostic product development or related areas would be an advantage. Ideally candidates will also have experience in immunoassay automation. Ideally educated to degree standard (or equivalent) in biochemistry or a related discipline, candidates will have a flexible approach, and be used to working under pressure on several tasks simultaneously, often on their own initiative. We would also consider candidates without a degree if they had directly relevant work experience. The salary for this job will be within the range £10,500 - £14,000 per annum, depending on age, experience and qualifications. The job will be based in Ely (Cambs. U.K.) and company benefits include: 25 days annual holiday, private medical insurance, permanent health insurance and life assurance. If you would like to apply for this job, please write before Wednesday 7th February, enclosing your C.V. to: Miss Jackie Stone, Personnel Officer, DAKO Diagnostics Ltd. Denmark House, Angel Drove, Ely CB7 4ET Cambridgeshire UK Tel: +44 1353 669911 Fax: +44 1353 668989 Sorry, snail-mail applications only please. From owner-diagnostics@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!newsspool.doit.wisc.edu!post.its.mcw.edu!usenet From: "Jennifer L. Potter" Newsgroups: bionet.agroforestry,bionet.audiology,bionet.biophysics,bionet.cellbiol,bionet.diagnostics,bionet.general,bionet.immunology,bionet.microbiology Subject: Re: Survey of women in science Date: 25 Jan 1996 02:12:58 GMT Organization: Dept. of Biochemistry, The Medical College of WI Lines: 6 Message-ID: <4e6ova$6o6@post.its.mcw.edu> References: NNTP-Posting-Host: d350-1.biochem.mcw.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) To: nberman@ucla.edu Xref: biosci bionet.agroforestry:2544 bionet.audiology:1136 bionet.biophysics:1628 bionet.cellbiol:3855 bionet.diagnostics:576 bionet.general:19544 bionet.immunology:7326 bionet.microbiology:4683 You may also want to cross-post to the bionet.women-in-bio group. Regards, Jennifer Potter From owner-diagnostics@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!gpu3!mmulvey From: mmulvey@gpu3.srv.ualberta.ca (Michael Mulvey) Newsgroups: bionet.diagnostics Subject: WHONET Date: 25 Jan 1996 08:03:19 GMT Organization: University of Alberta Lines: 13 Message-ID: <4e7dg7$8c2@pulp.ucs.ualberta.ca> NNTP-Posting-Host: gpu3.srv.ualberta.ca X-Newsreader: TIN [version 1.2 PL2] Hi everybody: I am requesting some information regarding the computer program WHONET. If anybody has an opinion about this program or suggestions about alternative software please write to me at mmulvey@gpu.srv.ualberta.ca. WHONET is a program to monitor the patterns of bacterial resistance in clinical micro labs. Thank you in advance. Dr. Mike Mulvey From owner-diagnostics@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: Dan Tisone Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal Subject: WEB SITE - Equipment for rapid diagnostic test kits Date: Wed, 24 Jan 1996 19:02:50 -0800 Organization: Netcom Lines: 3 Message-ID: <3106F2DA.2E47@ix.netcom.com> NNTP-Posting-Host: irv-ca5-22.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Wed Jan 24 7:00:47 PM PST 1996 X-Mailer: Mozilla 2.0b5 (Win95; I) Xref: biosci bionet.diagnostics:577 bionet.diagnostics.prenatal:105 There is a company, BioDot, who manufactures equipment for the rapid test kit market. Their web site, http://www.biodot.com/testkit, has pictures of some of their products if anyone is interested. From owner-diagnostics@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!data-transport.com!scott_jokerst From: scott_jokerst@data-transport.com (R. Scott Jokerst) Newsgroups: bionet.diagnostics Subject: Another source for product and services searching Date: 26 Jan 1996 15:13:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello Gwen, Another emerging resource for searching for biology related products and services is: http://www.data-transport.com Biological Data Transport, having recently acquired and upgraded its Biotech Registry, has expanding its services and is becoming a nice resource for companies to place their products and services for central access by a global scientific audience. It has been growing rapidly, and is becoming more comprehensive weekly. Fast, different, effective. A nice addition to bookmark. Scott ---> R. Scott Jokerst scott_jokerst@data-transport.com ---> ---> Biological Data Transport http://www.data-transport.com ---> ---> 510-648-8229 510-648-8279 (FAX) ---> From owner-diagnostics@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!alpha.sky.net!news From: "Gwen E. Sprague" Newsgroups: bionet.diagnostics Subject: Re: pregnancy tests Date: 26 Jan 1996 15:43:44 GMT Organization: SkyNET Online Lines: 17 Message-ID: <4easrg$4u0@alpha.sky.net> References: <4e9gof$nv8$1@mhadg.production.compuserve.com> NNTP-Posting-Host: ip23.sky.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:4e9gof$nv8$1@mhadg.production.compuserve.com Try searching here: http://www.biosupplynet.com/bsn/ This web sight is an excellent source for searches for products. Gwen <*><*><*><*><*><*><*><*><*><*><*><*> >>> Freelance Technogoddess <<< "I am NOT unemployed, I'm a consultant." <*><*><*><*><*><*><*><*><*><*><*><*> From owner-diagnostics@net.bio.net Thu Jan 25 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Federal Job <76770.1243@CompuServe.COM> Newsgroups: bionet.diagnostics Subject: pregnancy tests Date: 26 Jan 1996 03:11:11 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 2 Message-ID: <4e9gof$nv8$1@mhadg.production.compuserve.com> need 10,000 card tests at $0.30/test. pl forward list of vendors etc From owner-diagnostics@net.bio.net Fri Jan 26 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in1.uu.net!news-relay.eworld.com!news-relay.eworld.com!not-for-mail From: smerves@eworld.com (Smerves) Newsgroups: bionet.diagnostics Subject: Sources for mononucleosis and autoimmune plasmas Date: 27 Jan 1996 13:25:54 -0800 Organization: eWorld Lines: 2 Sender: root@news-relay.eworld.com Message-ID: <4ee592$98v@hp5.online.apple.com> Reply-To: smerves@eworld.com (Smerves) NNTP-Posting-Host: hp5.online.apple.com I am looking for sources of human plasma/ serum positive for mononucleosis or autoimmune antibodies. Can anyone help? From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!sasa.gov.uk!browning From: browning@sasa.gov.uk (Isla Browning) Newsgroups: bionet.diagnostics Subject: aspartame Date: 29 Jan 1996 10:14:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi I recently read in a newspaper that aspartame, the low calorie sweetener, has been found to produce neurological disorders. Does anyone know anything about this? Isla From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!ED.SAC.AC.UK!ESA009 From: ESA009@ED.SAC.AC.UK ("Dr Rob Harling") Newsgroups: bionet.diagnostics Subject: mismatch binding proteins Date: 29 Jan 1996 08:23:25 -0800 Organization: Scottish Agricultural College Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601291623.IAA08286@net.bio.net> NNTP-Posting-Host: net.bio.net I've come across a technique for detecting mutations/polymorphisms using mismatch binding protein (E.coli MutS). Can anyone help me by providing a reference which would explain how this works? Many thanks Dr Rob Harling SAC (Scottish Agricultural College)/ University of Edinburgh West Mains Road Edinburgh EH9 3JG Scotland, UK tel: +44 (0)131 535 4000 fax: +44 (0)131 667 2601 e mail: esa009@ed.sac.ac.uk From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!SCRI.SARI.AC.UK!djones From: djones@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Conjugate/Block Buffers Date: 29 Jan 1996 06:21:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601291418.OAA11091@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net I'm presently doing some comparisons between different buffers to dilute my labelled antibody in, for use in ELISA. So far I have established so far is that the standard conjugate buffer that we use here (2% PVP, 0.2% Ovalbumin in PBS-T) can be improved upon with the addition of various other proteins such as BSA and Casein. I was wondering whether anyone was using a buffer that they have found to be better than anything else they have tried, or does anyone know any commercial suppliers of conjugate/block buffers that they have found to be any good. Thanks David D.A.C. Jones S.C.R.I. Invergowrie Dundee Scotland e-mail djones@scri.sari.ac.uk From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!gatech!newsfeed.internetmci.com!news.texas.net!gossip.pyramid.com!news.sedona.net!client10.sedona.net!user From: SMI@sedona.net (Dave Jensen) Newsgroups: bionet.diagnostics Subject: Manager of Operations Search, Diagnostics Date: Sun, 28 Jan 1996 15:32:29 -0700 Organization: Search Masters International Lines: 27 Message-ID: NNTP-Posting-Host: client10.sedona.net Our company has been retained to find a Manager of Operations for a western-US (not CA) client diagnostics company which is currently in the "development to scaleup" mode. Our client has a significant technology, with one product already on the market and creating revenues, and we seek to find the first of the professional managers who will carry these products up and into the marketplace. Skills and abilities include the development of manufacturing methods and procedures for GMP production of diagnostics, strong knowledge of regulatory compliance and Reg. Affairs, and the technical capacity to assist this company in problem solving at the manufacturing level. This manager will hire a number of support personnel, as well as be the key project manager in the move to larger facilities that will support eventual commercialization. If you have any suggestions of contacts that might be useful to us on this search, please consider giving me a call. We appreciate your help greatly, keep all leads confidential, and have a VERY LONG MEMORY for favors. Contact: Dave Jensen, Managing Director Search Masters International Five Hundred Foothills South, Suite #2 Sedona, AZ 86336 (520) 282-3553 Phone or (520) 282-5881 Fax From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!BEPC2.IHEP.AC.CN!wangyq From: wangyq@BEPC2.IHEP.AC.CN Newsgroups: bionet.diagnostics Subject: Lyme_Disease Date: 29 Jan 1996 10:14:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <0099D23E.4438AA60.178@bepc2.ihep.ac.cn> NNTP-Posting-Host: net.bio.net Dear netters: I want to buy antigen of Lyme Disease, could you please tell me who should I contact? Thank you. Wait for your reply. Best Regards, Wang Yuquan Beijing Cons Bio-Tech From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!SEDONA.NET!SMI From: SMI@SEDONA.NET (Dave Jensen) Newsgroups: bionet.diagnostics Subject: Operations Manager, Diagnostics Date: 29 Jan 1996 13:52:45 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello - My company has been retained to find a senior level diagnostics industry executive who can help move a client into manufacturing, as Operations Manager of this young company. The person we seek will have enough diagnostics industry experience (6-10 yrs) to help de-bug technical problems in the scaleup of new diagnostics and biosensors. A regulatory compliance exposure and past involvement in R/A efforts will be required. This company has a product on the market and is now enjoying revenues. Yet, they are still a company young enough to see incredible growth over the next few years. This manager will have a significant position in their management team, and enjoy the benefits of equity and competitive compensation. Your thoughts on contacts for my search would be most appreciated. Dave Jensen --- Dave Jensen, Search Masters International 500 Foothills South, Suite 2, Sedona, AZ 86336 (520) 282-3553 Phone (520) 282-5881 Fax Internet: davej@sedona.net / BIO-Online http://smi.bio.com/ From owner-diagnostics@net.bio.net Sun Jan 28 22:00:00 1996 Path: biosci!aol.com!MHughes77 From: MHughes77@aol.com Newsgroups: bionet.diagnostics Subject: Re: Conjugate/Block Buffers Date: 29 Jan 1996 15:53:36 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <960129184346_409574194@mail06.mail.aol.com> NNTP-Posting-Host: net.bio.net In a message dated 96-01-29 09:58:51 EST, Daivd Jones wrote: > >I'm presently doing some comparisons between different buffers to dilute >my labelled antibody in, for use in ELISA. So far I have established so >far is that the standard conjugate buffer that we use here (2% PVP, 0.2% >Ovalbumin in PBS-T) can be improved upon with the addition of >various other proteins such as BSA and Casein. I was wondering whether >anyone was using a buffer that they have found to be better than >anything else they have tried, or does anyone know any commercial >suppliers of conjugate/block buffers that they have found to be any good. > >Thanks >David > > Our R&D Department has developed a diluent buffer for stabilizing HRP conjugates that has allowed us to store our antibody conjugates for over one year at 4C, and for at least two months at room temperature, with no significant loss of activity. This proprietary formulation contains a protein, a detergent, antimicrobial agents, and other components. It has worked very well for us in our product line of rapid ELISA tests, and is now being evaluated by others for use in their microtiter plate ELISAs. We have recently decided to offer the conjugate stabilizing buffer for sale, for those seeking to improve the shelf-life of their HRP-based ELISAs. For more information, please contact me by e-mail. Mark Hughes Empyrean Diagnostics Inc. mhughes77@aol.com From owner-diagnostics@net.bio.net Mon Jan 29 22:00:00 1996 Path: biosci!data-transport.com!scott_jokerst From: scott_jokerst@data-transport.com (R. Scott Jokerst) Newsgroups: bionet.diagnostics Subject: Re: aspartame Date: 29 Jan 1996 16:19:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi Isla, Generally old news, though I haven't read anything on the topic recently. What I do know is that phenylalanine is one of two amino acid components of aspartame. If you're phenylketonuric, you shouldn't ingest the stuff. This rare condition is generally diagnosed at birth. Issue would be for children in utero, possibly, who may have the condition, but whose parents are unaware it. Pregnant mothers thus would do best to shy away from the stuff. It's a start, but I do not claim comprehensive knowledge on the topic. Scott ********** At 10:14 AM 1/29/96, Isla Browning wrote: >Hi >I recently read in a newspaper that aspartame, the low calorie >sweetener, has been found to produce neurological disorders. Does >anyone know anything about this? > >Isla ---> R. Scott Jokerst scott_jokerst@data-transport.com ---> ---> Biological Data Transport http://www.data-transport.com ---> ---> 510-648-8229 510-648-8279 (FAX) ---> From owner-diagnostics@net.bio.net Tue Jan 30 22:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: (Fwd) Promega va Roche Date: 31 Jan 1996 01:56:25 -0800 Organization: Scottish Agricultural Science Agency Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601310953.aa13534@jura.sasa.gov.uk> Reply-To: odonnell@sasa.gov.uk NNTP-Posting-Host: net.bio.net I posted this two days ago and by the miracle of the information super-highway it promptly disappeared. Take 2: The latest from the Promega Home page (http://www.promega.com) is that the trial to contest the taq polymerase patent will start late January/early February. Both parties have agreed to separate the patent issue from a second issue, that of the right of scientists to use patented methods for research purposes. Roche are still contesting this and it will be the subject of a second trial. Meanwhile, Roche have just lost a case against Organon-Teknika. They claimed that Organon's NASBA HIV test breached the PCR patent and lost in a Dutch court. (Thi