From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!dobie.loop.com!KATHY
From: kcarlson@loop.com (Kathy Carlson)
Newsgroups: bionet.diagnostics
Subject: Re: aspartame
Date: Fri, 02 Feb 96 00:12:08 GMT
Organization: The Loop
Lines: 13
Distribution: world
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In article <MAPI.Id.0016.00726f776e696e673044334430303038@MAPI.to.RFC822>,
   browning@sasa.gov.uk (Isla Browning) wrote:
>Hi
>I recently read in a newspaper that aspartame, the low calorie 
>sweetener, has been found to produce neurological disorders. Does 
>anyone know anything about this?
>
>Isla
>
This web site has all the information:
www.tiac.net/users/mgold/aspartame/aspartame.html

Kathy

From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
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From: gwade@helixvc.com
Newsgroups: bionet.diagnostics
Subject: Help--Morphometrics/Auto Pap Analysis
Date: Thu, 01 Feb 1996 20:45:57 GMT
X-Newsreader: Forte Free Agent 1.0.82
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Does anyone know where this company is located.  I understand they
have devices for automated pap smear analysis.  I need to get info on
them. 

Any and  all info for this company and this market appreciated.

Sinceerely

Gregory R., Wade
Associate
Helix Investments Canada Inc.

Gwade@helixvc.com




From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.chatlink.com!news.erie.net!news
From: lconfer@moose.erie.net (Leslie Confer)
Newsgroups: bionet.diagnostics
Subject: Re: (Fwd) Promega va Roche
Date: Fri, 02 Feb 96 00:55:27 GMT
Organization: ErieNet
Lines: 27
Message-ID: <4erndv$a24_002@news.erie.net>
References: <9601310953.aa13534@jura.sasa.gov.uk> <Pine.A32.3.91.960131093002.62101C-100000@spider.usp.br>
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In article <Pine.A32.3.91.960131093002.62101C-100000@spider.usp.br>,
   lpereira@usp.br (Lygia da Veiga Pereira) wrote:
>
>Does anyone know of Organon's address (e-mail, fax, etc)?
>
>Thanks, 
>
>Lygia V. Pereira


Here are some snail mail addresses:

Organon Teknika Corp.
100 Akzo Ave.
Durham, N.C. 27712

Organon Teknika Corp.
1230 Wilson Drive
West Chester, PA  19380

I'll see if I can dig up some E-Mail, Fax, and phone numbers.



------------------------------
Les Confer
lconfer@moose.erie.net

From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.chatlink.com!news.erie.net!news
From: lconfer@moose.erie.net (Leslie Confer)
Newsgroups: bionet.diagnostics
Subject: Re: Conjugate/Block Buffers
Date: Fri, 02 Feb 96 00:33:47 GMT
Organization: ErieNet
Lines: 54
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> 
>I think that maybe I have misled people a bit with my first message.
>Which after reading it again probably isn't all that clear. What I am
>after is are buffers that will increase the signal : noise ratio in 
the
>actual ELISA. My actual Alkaline Phosphatase conjugates Pabs or mabs 
are
>quite stable enough. I don't really have any problems, I'm just 
trying
>to increase the sensitivity of the ELISA. By changing the buffer I
>dilute my conjugates in, I have managed a 1.5 - 2 times increase in
>senstivity. Not spectacular I know but it gives me the encouragement 
to go
>on and find better buffers if they exist. What I'd really like is a 
well
>tested commercial buffer, that I can use to see how my various 
mixtures
>compare.
>	Thanks
>	David
>

I really don't know much about commercial diluents.  There are quite
a few out there.  I've always made my own.  I do know that the source
and purity of stabilizing protein can have a great effect on S/N.
You might want to try using fatty acid free BSA, or fetal calf serum 
in your diluent.  Concentration and variations from lot to lot could 
have an effect. Variations in buffer ionic strength and pH could also 
have an effect.  

I guess a commercial supplier may be the best choice if you don't want 
to worry about variations.  You may want to try Kirkengard and Perry 
Labs, Pierce Chemical, BioRad, BioWhittaker, Sigma BioScience, to name 
just a few.  There is also a new biotechnology suppliers Web site at
http:/www.iscpubs.com.  However, even commercial buffers may differ 
depending on your antibody-antigen interactions and kinetics.  Most
suppliers are willing to provide samples for evaluation without charge
(if you're kind to the rep and say please).

Another possibility is changing washing buffers.  Is non-specific
binding a problem that reduces sensitivity?  If so, you may want to
try increasing wash cycle times, increase NaCl concentration, or try 
an alternative surfactant.  These all are interdependant, so be 
careful.  Also, have you thought about going to an avidin-biotin
conjugate.  This can significantly increase sensitivity.

This is all I can really say without more specific information and 
hard data.  Please E-Mail me if you have any questions.

Best regards,
Les Confer

lconfer@moose.erie.net


From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
Path: biosci!SCRI.SARI.AC.UK!djones
From: djones@SCRI.SARI.AC.UK (D Jones)
Newsgroups: bionet.diagnostics
Subject: Re: Conjugate/Block Buffers
Date: 2 Feb 1996 06:14:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Vigfrid Ness wrote:-
> 
> My comment is: Have you tried the NAD/H amplification system for AP-conjugates.
> My own experience with it isn't the best - the system was too sensitive when
> it came to background noice but I know about people in Wageningen who has
> had good experience with it for detection of plant pathogens.
> 
 Thanks for your comment. I have in fact tried the NAD/H amplification
system, and my experience was exactly the same as yours. The situation
was even worse when I tried to use tuber sap as opposed to leaf sap. I
have also used a luminescence based assay, which did actually prove to
be about 10 x more sensitive at it's best, but the Titanium oxide plates
I purchased from Dynatech were very unreliable and tended to go of over
time! 
	Has anyone else had any similar problems?  

				Dave
D.A.C. Jones
S.C.R.I.
Virology
Invergowrie
Dundee
DD2 5DA

e-mail: djones@scri.sari.ac.uk

From owner-diagnostics@net.bio.net Thu Feb 01 22:00:00 1996
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From: davecr@ix.netcom.com (David Crisp)
Newsgroups: bionet.diagnostics
Subject: QC sampling methods
Date: Fri, 02 Feb 1996 15:04:05 -0800
Organization: Netcom
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X-NETCOM-Date: Fri Feb 02  3:01:03 PM PST 1996

Hello all.

I'm trying to find a valid alternative to the MIL-STD 105E tables to
develop a QC sampling plan.  (If you haven't heard of these, thank your
stars).  A manufacturing group for which I am consulting thinks the
material consumption rate is too high with the use of the MIL-STD tables. 
I'm stuck with the task of coming up with an alternative method that has
some statistical basis (I'm not a statistician by any stretch of the
imagination).

Thanks in advance for any suggestions.

-- 
David Crisp

>>Slower traffic keep right>>

From owner-diagnostics@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!torn!news.bc.net!news.mindlink.net!line184.nwm.mindlink.net!Innovatek
From: Innovatek@mindlink.bc.ca (Innovatek Medical Inc.)
Newsgroups: bionet.diagnostics
Subject: Re: pregnancy tests
Date: Sat, 3 Feb 1996 09:05:11 GMT
Organization: MIND LINK! - British Columbia, Canada
Lines: 22
Message-ID: <Innovatek.1.31132547@mindlink.bc.ca>
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In article <4e9gof$nv8$1@mhadg.production.compuserve.com> Federal Job <76770.1243@CompuServe.COM> writes:
>From: Federal Job <76770.1243@CompuServe.COM>
>Subject: pregnancy tests
>Date: 26 Jan 1996 03:11:11 GMT

>need 10,000 card tests at $0.30/test. pl forward list of vendors 
>etc


We can supply dip-and-read strip pregnancy test at this price.  Not for sale 
in USA.

Contact us at:

	Innovatek Medical Inc.
	150 - 13500 Maycrest Way,
	Richmond, B.C.
	Canada.  V6V 2N8

	Tel: (604) 278-8886
	Fax: (604) 278-7999
	E-mail: INNOVATEK@mindlink.bc.ca

From owner-diagnostics@net.bio.net Fri Feb 02 22:00:00 1996
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.diagnostics
Subject: BIOSCI miniFAQ, ver. 14-DEC-95
Date: 3 Feb 1996 02:01:11 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 199
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(LAST REVISION: 14-DEC-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

	Contents:
	--------
	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index in addition to the master index for the entire set.  The main
BIOSCI home page also has access to the BIO-JOURNALS Table of Contents
database WAIS index and the BIOSCI user address database described in
another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
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   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
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   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				biosci-help@net.bio.net

From owner-diagnostics@net.bio.net Sat Feb 03 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.crl.com!newshub.cts.com!usenet
From: b3748@cts.com (Bryan Kiehl)
Newsgroups: bionet.diagnostics
Subject: Re: QC sampling methods
Date: Sun, 04 Feb 1996 06:58:31 GMT
Organization: CTS Network Services
Lines: 38
Message-ID: <4f1ld7$3m8@news2.cts.com>
References: <davecr-0202961504050001@ix-sj11-14.ix.netcom.com>
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davecr@ix.netcom.com (David Crisp) wrote:

The current standards are now published through the US QC
organization. The words are changed slightly, but in most respects the
two are the same. I do find the word changes make the tables easier to
use.

As an alternative, you might try your own sampling plans. However, you
should end up with much the same, since all this is based on
probability. I suggest that you get a copy of the new standards and
read carefully. If you can accept the lower confidence, you can use
these to do just about anythin.

Just remember, your not test for a regulatory agency, your testing for
you company to avoid costly errors. You need to make cost benefit
decisions that work best for you.

>Hello all.

>I'm trying to find a valid alternative to the MIL-STD 105E tables to
>develop a QC sampling plan.  (If you haven't heard of these, thank your
>stars).  A manufacturing group for which I am consulting thinks the
>material consumption rate is too high with the use of the MIL-STD tables. 
>I'm stuck with the task of coming up with an alternative method that has
>some statistical basis (I'm not a statistician by any stretch of the
>imagination).

>Thanks in advance for any suggestions.

>-- 
>David Crisp

>>>Slower traffic keep right>>

Bryan Kiehl
b3748@cts.com
San Diego, CA


From owner-diagnostics@net.bio.net Sat Feb 03 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!newsfeed.pitt.edu!bb3.andrew.cmu.edu!casaba.srv.cs.cmu.edu!das-news2.harvard.edu!fas-news.harvard.edu!fas.harvard.edu!rickles
From: rickles@fas.harvard.edu (Richard Rickles)
Newsgroups: bionet.cellbiol,bionet.diagnostics,bionet.drosophila
Subject: Fentomole Protein Sequencing
Date: 4 Feb 1996 15:02:09 GMT
Organization: Harvard University, Cambridge, Massachusetts
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X-Newsreader: NN version 6.5.0 #3 (NOV)
Xref: biosci bionet.cellbiol:3936 bionet.diagnostics:612 bionet.drosophila:1827


FEMTOMOLE PROTEIN SEQUENCING-Means you CAN identify thos proteins on PVDF 
from
2D gels and minigels...  

contact brauer@ariad.com (include your fax number)





From owner-diagnostics@net.bio.net Sat Feb 03 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-freiburg.de!mibm1.ruf.uni-freiburg.de!krieger
From: krieger@mibm1.ruf.uni-freiburg.de (Jens Krieger)
Newsgroups: bionet.diagnostics
Subject: staining of phosphoproteins
Date: 4 Feb 1996 15:43:40 GMT
Organization: Rechenzentrum der Universitaet Freiburg, Germany
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Hello!
I'm seeking for an method to stain very simple high phosphorylated 
proteins on SDS-acrylamide-gels. There exist a method of Cutting and Roth 
(1973) by staining them with methylgreen. Does anyone knows an exact 
"recipe" for doing it this way?
Thanks in advance,
			Jens.

Jens Krieger
Institute of Zoology, TU Dresden, Germany
e-mail: krieger@rcs1.urz.tu-dresden.de
Tel.: +49.351.463.5465
Fax.: +49.351.463.7093

From owner-diagnostics@net.bio.net Sun Feb 04 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: Pete Kwasowski <P.Kwasowski@surrey.ac.uk>
Newsgroups: bionet.diagnostics
Subject: Re: Conjugate/Block Buffers
Date: 5 Feb 1996 09:54:27 -0000
Lines: 40
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4f4k4j$dl@mserv1.dl.ac.uk>
X-Sender: bss3pk@central.surrey.ac.uk
MIME-Version: 1.0
Original-To: diagnost@dl.ac.uk

At 06:14 02/02/96 -0800, you wrote:
>I have also used a luminescence based assay, which did actually prove to
>be about 10 x more sensitive at it's best, but the Titanium oxide plates
>I purchased from Dynatech were very unreliable and tended to go of over
>time! 
>	Has anyone else had any similar problems?  
>
>				Dave



We have been using Enhanced Luminescence ELISA's for some time now, we have
found the Dynatech plates to be the best around (so far). However, we
exclusively use the Microlite 2 plates.

The Fluorescence and Microlite 1 plates give much lower signals (in our
hands). The strip-format plates seemed to give much poorer cv's (again, in
our hands).

Pete


BTW, does anyone know of a cheaper source high performance "white plates".


Dr P Kwasowski
ClifMar Associates Limited
School Biological Sciences
University of Surrey
Guildford, GU2 5XH
United Kingdom

Tel/FAX: +44 (0)1483 257908
E-mail: P.Kwasowski@surrey.ac.uk
http://www.surrey.ac.uk/SBS/comps/clifmar.html

                                     _\\|//_
                                     (-@-@-)
/----------------------------------ooO-(_)-Ooo-------------------------------\


From owner-diagnostics@net.bio.net Sun Feb 04 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.dacom.co.kr!news.kreonet.re.kr!usenet.kornet.nm.kr!ames!news.tulane.edu!usenet
From: "David M. Sander" <dmsander@mailhost.tcs.tulane.edu>
Newsgroups: bionet.diagnostics
Subject: Silicone Breast Implants and Disease
Date: Mon, 05 Feb 1996 10:45:05 +0000
Organization: Tulane University
Lines: 7
Message-ID: <3115DFB1.343E@mailhost.tcs.tulane.edu>
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Information about a diagnostic test under development in the Garry 
Laboratory is available at:

	http://www.tulane.edu/~dmsander/apa.html

The test is called the "Anti-Polymer Antibody Test", and is only 
available for research purposes.

From owner-diagnostics@net.bio.net Mon Feb 05 22:00:00 1996
Path: biosci!AGERI.SCI.EG!idl
From: idl@AGERI.SCI.EG
Newsgroups: bionet.diagnostics
Subject: new collegue
Date: 5 Feb 1996 23:44:56 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 25
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I am a new subscriber in this newsgroup, and this is the first E-mail.My 
study about the molecular characterization of the root-knot nematodes 
(Meloidogyne).I have just started my study, but there is some problem 
with me to extract the DNA from the nematodes so:
	if any one have an easy method to this extraction please send
	it to me. 
	, and if any one faced him some problem with extracting DNA and 
	He found the solutions. it may be useful to me if he can send it 
	to me.
	also I have some questions:
Can I use Polyvenylpyrollydone(PVP M.W. 360 000) instead of 
Polyvenylpyrollidone (PVPP) to prepare a buffer to extract DNA [it 
consists of EDTA, Sarcosyl, PVPP(water insoluble)?
How could I sterilize the PVP, and Sarcosyl, could I autoclaved it?  
 
SORRY for this too long E-mail, but all of these questions is very 
important to me.Thanks for your cooperation.
							Abd elnasser

Abd Elnasser Elashry 
Agricultural genetic Engineering Research Institute(AGERI)
Agricultural Research Center(ARC)
9 Gamaa st., Giza, Egypt
E-mail: idl@ageri.sci.eg


From owner-diagnostics@net.bio.net Wed Feb 07 22:00:00 1996
Path: biosci!BIOBASE.DK!thorup
From: thorup@BIOBASE.DK (Jan Ulrik Thorup)
Newsgroups: bionet.diagnostics
Subject: 11th International Symposium on Regulatory Peptides
Date: 8 Feb 1996 02:42:07 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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	11th International Symposium on Regulatory Peptides

		(Formerly Gastrointestinal Hormones)

	Copenhagen, Denmark			September 3-7, 1996
-------------------------------------------------------------------------------

The Symposium will discuss hormones, neuropeptides and paracrine peptides from
molecular and cell biology, secretion, receptors and pharmacology to their
role in cancer, neuropsychiatric, gastrointestinal and endocrine disorders.


Plenary speakers:

	Sir James Black, London (Heptahelix receptors)
	Jeffrey M. Friedman, New York (Peptides and obesity)
	Carl-Henrik Heldin, Uppsala (PDGF and TGFb in growth control)
	Wieland Huttner,Heidelberg(Secretory granules in neuroendocrine cells)
	Gilbert Vassart, Brussels (Receptor mutants and disease)


In addition 150 shorter oral and 300 poster presentations will be selected
from submitted abstracts (deadline April 1, 1996)
-------------------------------------------------------------------------------

Organizers:				Information and registration:

Jens F. Rehfeld				Symposium on Regulatory Peptides
Linda Hilsted				c/o DIS Congress Service Copenhagen
Linda Bardram				Herlev Ringvej 2C
Jan Fahrenkrug				DK-2730 Herlev, Denmark
Jens Juul Holst				Phone;+45 44 92 44 92
Cathrine Oerskov			Fax;+45 44 92 50 50







From owner-diagnostics@net.bio.net Wed Feb 07 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!news.compuserve.com!news.production.compuserve.com!news
From: Leslie Boux <73302.706@CompuServe.COM>
Newsgroups: bionet.diagnostics,bit.med.mxdiag-l,sci.med,sci.med.pharmacy
Subject: Blood Collection Tubes
Date: 8 Feb 1996 14:23:49 GMT
Organization: CompuServe, Inc. (1-800-689-0736)
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Xref: biosci bionet.diagnostics:619 sci.med:109644 sci.med.pharmacy:22010

Hi anyone,
   I am looking for names (and addresses if posssible) of any 
manufacturer of blood collection tubes (other than BD).  If you have
any info would you please reply to this message.
   Thanks, Leslie Boux  (73302.706@compuserve.com)

From owner-diagnostics@net.bio.net Wed Feb 07 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!alpha.sky.net!news
From: "Gwen E. Sprague" <gsprague@sky.net>
Newsgroups: bionet.diagnostics,bit.med.mxdiag-l,sci.med,sci.med.pharmacy
Subject: Re: Blood Collection Tubes
Date: 8 Feb 1996 16:48:40 GMT
Organization: SkyNET Online
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Leslie Boux <73302.706@CompuServe.COM> wrote:
>Hi anyone,
>   I am looking for names (and addresses if posssible) of any 
>manufacturer of blood collection tubes (other than BD).  If you have
>any info would you please reply to this message.
>   Thanks, Leslie Boux  (73302.706@compuserve.com)

Leslie,

There are several makers of blood collection tubes, BD(vaccutainer), Sherwood Medical 
(Monoject?), Venoject. They can be obtained thru suppliers like CMS or Baxter or from the 
company direct. Below are a couple of websites you can search for more information. 

http://www.biosupplynet.com/bsn/

http://www.data-transport.com/


Good Luck!

Gwen



<*><*><*><*><*><*><*><*><*><*><*><*>
        >>> Freelance Technogoddess <<<
  "I am NOT unemployed, I'm a consultant."
<*><*><*><*><*><*><*><*><*><*><*><*>



From owner-diagnostics@net.bio.net Thu Feb 08 22:00:00 1996
Path: biosci!bmg.corange.com!EBERHARD_RUSSMANN
From: EBERHARD_RUSSMANN@bmg.corange.com ("EBERHARD RUSSMANN")
Newsgroups: bionet.diagnostics
Subject: rare cell isolation
Date: 9 Feb 1996 01:37:08 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Post Newsgroup: DIAGNOSTICS/bionet.diagnostics Newsgroup Archive

Subject: Rare Cell Isolation

I want to separate rare cells (tumor cells, stem cells) from human peripheral 
blood. Did anyone compare the various cell separation systems (DYNAL, IMMUNICON,
CELLPRO, MILTENYI/MACS) and can give me a hint which separation system will fit 
best for this purposes?

Thanks

Eberhard Russmann
e-mail:  eberhard_russmann@bmg.corange.com 


From owner-diagnostics@net.bio.net Sat Feb 10 22:00:00 1996
Path: biosci!agate!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold
From: Bert Gold <bgold@itsa.ucsf.edu>
Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal
Subject: PRENATAL TRIPLE TEST
Date: Sat, 10 Feb 1996 17:04:05 -0800
Organization: UCSF, ITS
Lines: 39
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Dear Netters,


1) The ACOG Guidelines [1] and Guidelines for Perinatal Care published
   by a joint committee of ACOG and the American Academy of Pediatrics [2]
   are very out of date.

2) The triple screen, in capable hands, should detect 60% of antenatal
   Down's Syndrome births, 70% of chromosome 18 trisomies, significant
   proportions of Turner's and all patent Open Neural Tube Defects.

3) Newer versions of the Screening Test [3] will detect 72% of Down's
   Syndrome gestations with a 2-5% false positive rate.

4) For these reasons, an international collaboration has strongly
   endorsed the policy of offering testing to ALL women [4].


Bert Gold, Ph.D.
University of California, San Francisco
School of Medicine
Board Active Status - (Eligible) - Medical Molecular Genetics
American Board of Medical Genetics

REFERENCES

[1] American College of Obstetricians and Gynecologists, Standards for
    Obstetric-Gynecologic Services, 7th Edition, 1989.

[2] American Academy of Pediatrics and American College of Obstetricians
    and Gynecologists, Guidelines for Perinatal Care, 2nd Edition, 1988.

[3] Prenatal Diagnosis (1994) 14: 707-716 and 14: 717-719.


[4] Wald, NJ; Kennard, A.; Watt, H.; Haddow, J.E.; Palomaki, G.E.; Knight,
    G. and Canick, J.A. (1995) Decision Analysis and Screening for Down's
    Syndrome.  Testing Should be in All Women.  BMJ 311, 1372-1373

From owner-diagnostics@net.bio.net Sat Feb 10 22:00:00 1996
Path: biosci!news.Stanford.EDU!lll-winken.llnl.gov!news.larc.nasa.gov!lerc.nasa.gov!magnus.acs.ohio-state.edu!math.ohio-state.edu!howland.reston.ans.net!swrinde!newsfeed.internetmci.com!news.chatlink.com!news.erie.net!news
From: lconfer@ncinter.net (Les Confer)
Newsgroups: bionet.diagnostics,bit.med.mxdiag-l,sci.med,sci.med.pharmacy
Subject: Re: Blood Collection Tubes
Date: Sun, 11 Feb 1996 06:51:50 GMT
Organization: ErieNet
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Xref: biosci bionet.diagnostics:623 sci.med:110008 sci.med.pharmacy:22171

Leslie Boux <73302.706@CompuServe.COM> wrote:

>Hi anyone,
>   I am looking for names (and addresses if posssible) of any 
>manufacturer of blood collection tubes (other than BD).  If you have
>any info would you please reply to this message.
>   Thanks, Leslie Boux  (73302.706@compuserve.com)

Terumo Medical makes the Venoject line of blood collection
tubes.  They are located on Blueball Road in Elkton,
Maryland.  



From owner-diagnostics@net.bio.net Sun Feb 11 22:00:00 1996
From: Voyager_Technology@msn.com (Arthur V. Stein)
Subject:  jh
Date: 12 Feb 96 01:54:41 -0800
Message-ID: <00005ae5+00000196@msn.com>
Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.sprintlink.net!news.msn.com!msn.com
Newsgroups: bionet.diagnostics
Organization: The Microsoft Network (msn.com)
Lines: 1

ty

From owner-diagnostics@net.bio.net Sun Feb 11 22:00:00 1996
Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!tank.news.pipex.net!pipex!demon!user
From: filter@firthcom.demon.co.uk (Steve Firth)
Newsgroups: bionet.diagnostics
Subject: Re: Your Proposal for International Policy For Maternal Testing
Date: Mon, 12 Feb 1996 23:04:45 +0000
Organization: Firth Consulting
Lines: 18
Message-ID: <AD45780D966828FF34@firthcom.demon.co.uk>
References: <D224ZWDYHZH9O*.R=FDADR.R=A1.U=KJF.@MHS> <Pine.A32.3.91.960212105159.125110A-100000@itsa.ucsf.edu>
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In article <Pine.A32.3.91.960212105159.125110A-100000@itsa.ucsf.edu>,
bgold@ITSA.UCSF.EDU (Bert Gold) wrote:

>YES.  I strongly support the universal offer of such prenatal testing.
>And with the help of the international genetics community, I am prepared
>to defend my views before any person or body, no matter how august.

You may be interested to know that in 1994 I edited a monograph on the
subject of prenatal screening for Down's syndrome. The monograph was
published by Abbott Diagnostics and I believe that it is available FOC to
interested parties. A number of papers were given by experienced European
clinicians and laboratory scientists and the balance of opinion was in
favour of application of pre-natal screening for women under the age of 40.

Stephen Firth, Firth Consulting, UK.



From owner-diagnostics@net.bio.net Sun Feb 11 22:00:00 1996
Path: biosci!ITSA.UCSF.EDU!bgold
From: bgold@ITSA.UCSF.EDU (Bert Gold)
Newsgroups: bionet.diagnostics
Subject: Re: Your Proposal for International Policy For Maternal Testing
Date: 12 Feb 1996 10:59:23 -0800
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NNTP-Posting-Host: net.bio.net



YES.  I strongly support the universal offer of such prenatal testing.
And with the help of the international genetics community, I am prepared
to defend my views before any person or body, no matter how august.


Bert Gold, Ph.D.
University of California, San Francisco
School of Medicine
Program in Medical Genetics


From owner-diagnostics@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!daresbury!nntp-trd.UNINETT.no!nac.no!ifi.uio.no!nntp.uio.no!news.cais.net!primus.ac.net!imci4!newsfeed.internetmci.com!news.compuserve.com!newsmaster
From: <100073.243@compuserve.com>
Newsgroups: bionet.diagnostics,bionet.diagnostics.prenatal
Subject: Prenatal Triple Test
Date: 13 Feb 1996 19:12:06 GMT
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Dear Bert,

What's your opinion about the new Inhibin-A test as a screening for Down ?
This test could be performed in the mothers blood and in an earlier stage according
to some publications of prof. Groome (UK).

Peter D.J. Langens

From owner-diagnostics@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!agate!hpg30a.csc.cuhk.hk!news.cuhk.edu.hk!newsfeeder.ust.hk!nntp.hk.super.net!news.iij.ad.jp!fu.bekkoame.or.jp!suika!usenet
From: sugisaki@katanuki.bekkoame.or.jp (sugisaki)
Newsgroups: bionet.diagnostics
Subject: carbohydrate deficient transferrin (CDT)
Date: 13 Feb 1996 15:57:31 GMT
Organization: None
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Dear netters; It was said that serum level of carbohydrate deficient
transferrin might be a better diagnostic marker of chronic excessive
drinking (EtOH) regardless of with or without liver injury. However, I
failed to find out any possible diagnostic advantage of this marker
over a conventional marker such as ALT, AST and g-GTP.  I felt that
g-GTP was far better from view points of sensitivity and accuracy. I
appreciate to have some suggestions from netters on the marker, if any
other opinion. There is really an increasing social demand to have an
objective diagnostic marker of excessive drinking in particular in this
country.   

sugisaki@ppp.bekkoame.or.jp (H. Sugisaki)

From owner-diagnostics@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!GWH.BMI.AC.CN!yuyx
From: yuyx@GWH.BMI.AC.CN (Yu Yanxing)
Newsgroups: bionet.diagnostics
Subject: plasmid of CEA scFv help!
Date: 12 Feb 1996 17:11:25 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 12
Sender: daemon@net.bio.net
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Message-ID: <199602130110.JAA02555@gwh.bmi.ac.cn>
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Dear  sir   
 I am a postdoctoral fellow. Now I need the plasmid of CEA scFv during
my research. I am vondering if anyone can offer this plasmid. Thanks 
for your assistance.

                                          sincerely Yan-Xing Yu Ph.D.
                                          Depatment of General Surgery
                                          General Hospital of PLA
                                          28 Fu-Xing Road
                                          Beijing 100853 China
  


From owner-diagnostics@net.bio.net Mon Feb 12 22:00:00 1996
Path: biosci!MED.PITT.EDU!ehr
From: ehr@MED.PITT.EDU (Garth Ehrlich)
Newsgroups: bionet.diagnostics
Subject: testing for NF
Date: 13 Feb 1996 14:37:18 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Message-ID: <9602132235.AA22669@earth.med.pitt.edu>
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Dear Netters:

Can anyone tell me who is performing on a clinical basis DNA-based testing for neurofibromatosus.  My brother-in-law is affected and my sister would like to have her four children checked.


Sincerely

Garth D. Ehrlich, Ph.D
Director Core PCR Facility
University of Pittsburgh
Pittsburgh PA 15261
email ehr@med.pitt.edu

From owner-diagnostics@net.bio.net Tue Feb 13 22:00:00 1996
Path: biosci!bloom-beacon.mit.edu!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!in2.uu.net!news2.interlog.com!winternet.com!alpha.sky.net!news
From: "Gwen E. Sprague" <gsprague@sky.net>
Newsgroups: bionet.diagnostics
Subject: Re: carbohydrate deficient transferrin (CDT)
Date: 14 Feb 1996 00:10:35 GMT
Organization: SkyNET Online
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sugisaki@katanuki.bekkoame.or.jp (sugisaki) wrote:
>Dear netters; It was said that serum level of carbohydrate deficient
>transferrin might be a better diagnostic marker of chronic excessive
>drinking (EtOH) regardless of with or without liver injury. However, I
>failed to find out any possible diagnostic advantage of this marker
>over a conventional marker such as ALT, AST and g-GTP.  I felt that
>g-GTP was far better from view points of sensitivity and accuracy. I
>appreciate to have some suggestions from netters on the marker, if any
>other opinion. There is really an increasing social demand to have an
>objective diagnostic marker of excessive drinking in particular in this
>country.   
>
>sugisaki@ppp.bekkoame.or.jp (H. Sugisaki)



I have been involved in alcohol abuse markers over the past several years, comparing 
enzymes (ggt etc) with CDT, Beta-Hexosaminidase and Hemoblobin Acetaldehyde. GGT is 
actually a relative poor marker (sens in the mid 60s) but no one of the others are much 
better. Sometimes the best results achived are through the use of a combination of markers.

I think that problems seen with these markers are related to the wide variety of individual 
physiological reaction to alcohol consumption, the wide variability of drinking patterns, the 
broad definition of abuse and the variability of the indicators themselves. One person may 
show elevations of one marker over the other but it is not predictive of the results of that 
marker for another subject.

An other problem of some of the newer markers are the developmental stage of the 
technology available to perform the tests. No gold standard, fuzzy cutoffs and unclear 
interpretations confuse the issue even more.

There is a lot of literature out there (especially from the Nordic countries) but I don't think it 
will reduce your confusion. Speciality Labs in California are doing alot of work with CDT. You 
could contact them for some further information. Good Luck.


Gwen


<*><*><*><*><*><*><*><*><*><*><*><*>
        >>> Freelance Technogoddess <<<
  "I am NOT unemployed, I'm a consultant."
<*><*><*><*><*><*><*><*><*><*><*><*>



From owner-diagnostics@net.bio.net Tue Feb 13 22:00:00 1996
Path: biosci!greb.ulaval.ca!Christian.Mouton
From: Christian.Mouton@greb.ulaval.ca (Christian Mouton)
Newsgroups: bionet.diagnostics
Subject: Looking for slides: DNA probe hybridization
Date: 14 Feb 1996 13:19:24 -0800
Organization: UniversitŽ Laval
Lines: 9
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9602142117.AA25825@hermes.ulaval.ca>
NNTP-Posting-Host: net.bio.net

I am looking for a series of slides, intended for non-molecular 
biologists, describing the steps - from DNA denaturation to 
hybridization with target DNA - in order to explain the use of DNA 
probes. Has anyone ever encountered a WWW site where such JPEGvie 
wmaterial would be available?
Thanks and regards,




From owner-diagnostics@net.bio.net Wed Feb 14 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Re: Looking for slides: DNA probe hybridization
Date: 15 Feb 1996 01:47:01 -0800
Organization: Scottish Agricultural Science Agency
Lines: 28
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NNTP-Posting-Host: net.bio.net

On 14 Feb 96 at 13:19, Christian Mouton wrote:

> I am looking for a series of slides, intended for non-molecular 
> biologists, describing the steps - from DNA denaturation to 
> hybridization with target DNA - in order to explain the use of DNA 
> probes. Has anyone ever encountered a WWW site where such JPEGvie 
> wmaterial would be available?

Try the following sites:

Cells Alive! http://www.comet.chv.va.us/quill/

Access Excellence  http://www.gene.com:80/ae/

Primer on molecular genetics http://www.gdb.org/Dan/DOE/intro.html

Bugs in the news http://falcon.cc.ukans.edu/~jbrown/bugs.html


Those are all good educational sites dealing with molecular biology, 
though I can't recall what they have in the way of illustrations.

Kevin
 
Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Wed Feb 14 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!gatech!newsfeed.pitt.edu!godot.cc.duq.edu!usenet
From: "Jeffrey W. Clemens" <clemens@duq2.cc.duq.edu>
Newsgroups: bionet.diagnostics
Subject: Source for Progest. ELISA
Date: 15 Feb 1996 20:16:52 GMT
Organization: Duquesne University
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Does anyone know of a source for a microplate based progesterone ELISA?
I would appreciate any leads or contacts that you might be able to 
provide.

Direct responses would be appreciated and I could post results to group.

Thanks!

Jeffrey W. Clemens

clemens@duq2.cc.duq.edu



From owner-diagnostics@net.bio.net Wed Feb 14 22:00:00 1996
Path: biosci!AGERI.SCI.EG!idl
From: idl@AGERI.SCI.EG
Newsgroups: bionet.diagnostics
Subject: More Information
Date: 15 Feb 1996 04:53:10 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Is there anyone have any idea about the Email of the following researchers:
------>	H.J.S.Dawkins, and Terence spencer 
	Department of Agriculture and Rural Affairs, Regional Veterinary Lab.
	, Australia
------> J.D.Eisenback
	department of plant pathology Physiology and weed science, virginia
	polytechnic institute and state univ. 
------> J.N.Sasser 
	Department of plant pathology, North Carolina State Univ.
------> K.N.Lambert, or E.C.Tedford, or E.B.Caswell, or V.M.Williamson
	Department of Nematology, University of California, Davis.

------> IF ANYONE HAVE ANY E-MAIL WHICH BELONG TO ANY NEMATOLOGIST, PLEASE
	SEND IT TO ME.
						Yours 
					     Abd Elnasser
Agricultural Genetic Engineering research Institute 
Egypt	



From owner-diagnostics@net.bio.net Wed Feb 14 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Immunolink-AP Kits
Date: 15 Feb 1996 04:16:12 -0800
Organization: Scottish Agricultural Science Agency
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We used to use an immunolink-ap kit from Zeneca to produce our 
monoclonal antibody conjugates and it worked very well. Production of the 
kit has now moved from Zeneca to Genosys and we have experienced a
 sharp drop in quality. Has anyone else any experience of this kit?

Does anyone have any recommendations for conjugation kits?

Kevin
Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Wed Feb 14 22:00:00 1996
Path: biosci!biosci!not-for-mail
From: dbecker@ix.netcom.com (Daniel M. Becker)
Newsgroups: bionet.biology.cardiovascular,bionet.cellbiol,bionet.diagnostics,bionet.general,bionet.genome.chromosomes,bionet.immunology,bionet.metabolic-reg,bionet.microbiology,bionet.molbio.gdb,bionet.molbio.gene-linkage,bionet.molbio.hiv,bionet.molbio.methds-reagents,bionet.molbio.yeast,bionet.virology,bit.listserve.medforum
Subject: W. French Anderson seminar, June 1988: did you attend? (legal dispute involving patent rights)
Date: 15 Feb 1996 15:58:20 -0800
Organization: Netcom
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Xref: biosci bionet.biology.cardiovascular:787 bionet.cellbiol:4082 bionet.diagnostics:638 bionet.general:19938 bionet.genome.chromosomes:1043 bionet.immunology:7727 bionet.metabolic-reg:674 bionet.microbiology:4967 bionet.molbio.gdb:427 bionet.molbio.gene-linkage:978 bionet.molbio.hiv:1919 bionet.molbio.yeast:4740 bionet.virology:6227

In connection with a pending legal dispute involving patent rights to
human gene therapy, 

     I would like to identify all attendees of a June 1988 seminar
given by Dr. W. French Anderson at N.I.H. at which he described the
N2-TIL gene therapy protocol (nominally dated June 10, 1988) of which
he, R. Michael Blaese, and Steven A. Rosenberg were principal
investigators.  The Washington Post reported on Wednesday, June 15,
1988 that "[i]n a description of the experiment filed with government
officials this week, a research team including Dr. Steven A. Rosenberg
of the NCI and Dr. W. French Anderson. . . proposed inserting a
'marker' gene into a newly identified type of cancer-fighting cell and
then placing the altered cells into patients. . . . Anderson first
described the experiment at a seminar last week at NIH."

    If you attended this seminar or know someone who did, please
contact me via e-mail or snail mail:

Daniel M. Becker, M.D., J.D.
Pennie & Edmonds
2730 Sand Hill Rd, Suite 300
Menlo Park, CA  94022
dbecker@pennie.com

From owner-diagnostics@net.bio.net Thu Feb 15 22:00:00 1996
Path: biosci!ncl.ac.uk!Larry.Winger
From: Larry.Winger@ncl.ac.uk (Larry Winger)
Newsgroups: bionet.diagnostics
Subject: Re:Immunolink-AP Kits
Date: 16 Feb 1996 01:23:19 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <v01510101ad49fb424d97@[128.240.130.5]>
NNTP-Posting-Host: net.bio.net

We also have used both AP and HRP immunoconjugation kits from Cambridge
Research Biochemicals (as was, before being taken over by ICI and turning
into Zeneca?), and have purchased both from Genosys.  Our experience,
frankly, has been variable.

Alk-phos kits have 'worked', to date,  but we've had problems with amount
of reagent supplied, vials and components missing.  Also, because all kits
have to come from the American supplier (and are not always even 'in stock'
there), we've had quite serious delays in getting our orders filled.

In our most recent HRP conjugation, however, we had terrible coupling,
compared to a previous Genosys kit with the same antibody -- we didn't know
whether or not that was the fault of the kit, or our own problem, but it
does look worrisome -- we'll see how the second coupling (each kit comes
with nominal reagents for two coupling procedures) goes.


It would be interesting to hear from a Genosys representative in this
regard, who can reassure us on relevant QC questions!

                Larry   'wearing three hats in these cold times'     Winger

                     Larry.Winger@ncl.ac.uk     http://georgia.ncl.ac.uk
      LarryWinger@mkn.co.uk      http://mkn.co.uk/help/extra/craftnet/info
      law3@student.open.ac.uk (Larry A. Winger)   'Ed Resources on the Net'



From owner-diagnostics@net.bio.net Thu Feb 15 22:00:00 1996
Path: biosci!UNL.EDU!rdonis
From: rdonis@UNL.EDU (Ruben Donis)
Newsgroups: bionet.diagnostics
Subject: International BVD Virus Symposium, Announcement II
Date: 16 Feb 1996 09:06:16 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 192
Sender: daemon@net.bio.net
Distribution: world
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Announcement, Registration forms & updated info                   2-16-96
*************************************************************************
This announcement and further updates are also available on the WWW
http://web.vet.cornell.edu/public/DL/bvd.htm
*************************************************************************
                        INTERNATIONAL SYMPOSIUM

                                  50 YEARS
                        BOVINE VIRAL DIARRHEA VIRUS
                              June 23-25, 1996

                     SPONSORED BY PFIZER ANIMAL HEALTH
                                       at the
                NEW YORK STATE COLLEGE OF VETERINARY MEDICINE
                            AT CORNELL UNIVERSITY


Dear Colleague:

In recognition of the 50th anniversary of the "discovery" of a cattle
disease that is now known to be caused by BVD virus, an international
symposium will convene at Cornell University on June 23-25, 1996. The intent
of the symposium is to review the past 50 years of BVD research and to
attempt to set the agenda for future efforts to control this ruminant
pathogen. This meeting will cover all aspects of BVD virus including
historic perspectives, molecular biology of the virus, pathogenesis of acute
BVD virus infections as well as mucosal disease, epidemiology, diagnostics,
vaccines and other control strategies. The goal of the meeting is to provide
a comprehensive overview for everyone interested in the BVD virus disease
complex.

Through the generosity of a grant from Pfizer Animal Health, an
international panel of invited speakers will present the main program. In
addition, abstracts will be solicited from those who wish to present new
data from ongoing studies. These will be interspersed with the invited
presentations or selected for a poster session on Monday evening. Time will
be allotted for roundtable discussions to allow attendees to question and
comment on the presentations.

The meeting site will be the College of Veterinary Medicine with shuttle
service provided from local hotels to the College. The welcoming reception
on Sunday, lunches on Monday and Tuesday as well as dinner on Monday will be
provided at the Conference site.

We hope you will join us for the auspicious occasion.

     Organizing Committee:
     Steve Bolin
     Joe Brownlie
     Ruben Donis
     Edward Dubovi
     Rob Tremblay

TENTATIVE SCHEDULE

 Sunday, June 23    5-7 PM    Registration and Reception

                    7-10 PM   Historic overview

 Monday, June 24    8-5 PM    Molecular Biology and
                              Pathogenesis

 Tuesday, June 25   8-5 PM    Epidemiology, Diagnostics,
                              Vaccines, Control Strategies

                    7-10 PM   Banquet

Pre-registration for the meeting is required, with a registration fee of
$75.00.

Printable Registration form is available below or at the WWW site:
http://web.vet.cornell.edu/public/DL/bvd.htm

Accommodations will be provided by the local hotels and motels. Reservations
will need to be handled directly with the hotel of your choice. A block of
100 rooms have been set aside for the symposium at the Best Western and the
Sheraton Motor Inn. Early reservations are advisable given the limited
facilities in Ithaca.

Ithaca is "centrally isolated" in upstate New York. The Ithaca airport is
serviced by commuter airlines from Philadelphia and Newark and jet service
from Pittsburgh. The Syracuse airport is serviced by most major airlines and
is approximately 60 miles from Ithaca. Shuttle service can be arranged to
Ithaca.

ABSTRACTS

Abstracts are being solicited from those who wish to present data from
ongoing studies. The organizing committee will select those which most
complement the topics addressed by the invited speakers for an oral
presentation. The remaining abstracts will be assigned to the poster
session. If there is a preference for the poster session please note that on
your correspondence. The abstract should be restricted to 250 words. It
should be clearly noted as to who will present the paper should it be
selected for oral presentation. Multiple abstracts may be
submitted.abstracts. Publication of the proceedings is anticipated.
Accordingly, please submit five copies of the abstract along with a computer
disc containing the submitted document. Note the software program. The
deadline for submission of the abstracts is April 1, 1996.

Send abstracts to:

     Dr. Edward J. Dubovi
     Diagnostic Laboratory
     P.O. Box 5786
     Cornell University
     Ithaca, NY 14851-5786
     (607) 253-3900


                       INVITED SPEAKERS (partial list)

                                Eugene Berry
                                 Steve Bolin
                                 Kenny Brock
                                Joe Brownlie
                                Mark Collett
                                 Ruben Donis
                                 John Ellis
                                 Francis Fox
                                  Hans Houe
                               Peter Kirkland
                               Michael O'Hara
                                Juli Ridpath
                                Rob Tremblay
                               Hana Van Campen


Further information can be obtained from:

     Linda R. Alfreds
     College of Veterinary Medicine,
     T1 003 VRT, Cornell University,
     Ithaca, NY 14853-6401
     (607) 253-3198

     or send an e-mail message to:
     Dr. Edward J. Dubovi ejd5@cornell.edu

Program Updates will be posted to the WWW site periodically:
http://web.vet.cornell.edu/public/DL/bvd.htm
*******************************************************
REGISTRATION FORM

Mail To:
Linda R. Alfreds
Office of Continuing Education
College of Veterinary Medicine
Cornell University
Ithaca, NY 14853
Telephone 607-253-3200
Fax 607-253-3198

Please register me for the INTERNATIONAL SYMPOSIUM on 50 Years of Bovine
Viral Diarrhea Virus. I have enclosed a check or credit card number in the
amount of $75.00 (U.S. Funds) made payable to Cornell University.



First Name____________________________

Last Name____________________________

E-mail address______________________

Address______________________________

 _____________________________________

 City__________________________________

 State_________________________________

 Zip__________________________________

 Country_______________________________

 Telephone_____________________________

 Fax___________________________________

 Company/Institution_____________________

 Visa ( ) Mastercard ( ) Discover ( )

 Credit Card
 #_________________________
 Expiration
 Date________________________
( ) Yes, I will be attending the banquet on Tuesday evening.



From owner-diagnostics@net.bio.net Thu Feb 15 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Re:Immunolink-AP Kits
Date: 16 Feb 1996 05:50:03 -0800
Organization: Scottish Agricultural Science Agency
Lines: 30
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9602161346.aa00006@jura.sasa.gov.uk>
Reply-To: odonnell@sasa.gov.uk
NNTP-Posting-Host: net.bio.net

On 16 Feb 96 at 1:23, Larry Winger wrote:

> In our most recent HRP conjugation, however, we had terrible coupling,
> compared to a previous Genosys kit with the same antibody -- we didn't know
> whether or not that was the fault of the kit, or our own problem, but it
> does look worrisome -- we'll see how the second coupling (each kit comes
> with nominal reagents for two coupling procedures) goes.
> 
We use the kit to conjugate our monoclonal lines, reducing one 
potential source of variation. We are certain that 
the kit is at the root of the problem.

> It would be interesting to hear from a Genosys representative in this
> regard, who can reassure us on relevant QC questions!
> 

I agree. If anybody can supply an email address I'll invite them to 
comment.

>                 Larry   'wearing three hats in these cold times'     Winger
>
Not THE Larry Winger, the author?

Kevin

 
Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Thu Feb 15 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!dsi.unimi.it!sirio.cineca.it!gopher
From: "Prof. Giovanni Abatangelo" <abatange@civ.bio.unipd.it>
Newsgroups: bionet.diagnostics
Subject: Rat B langerhans cells
Date: 16 Feb 1996 11:12:32 GMT
Organization: Institute of Histology, University of Padova
Lines: 14
Message-ID: <4g1or0$aoc@sirio.cineca.it>
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Hi there, 

we are studying the langerhans islets in vitro seeded onto biomaterials.

Our question is: is there any marker (antibody or something else) for 
langerhans b cells of the rat (besides radiolabelled leu)?

Thank you in advance for your support.

Please reply directly to me: abatange@civ.bio.unipd.it

Marco Radice
University of Padova 


From owner-diagnostics@net.bio.net Thu Feb 15 22:00:00 1996
Newsgroups: bionet.diagnostics
Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!plug.news.pipex.net!pipex!tube.news.pipex.net!pipex!lade.news.pipex.net!pipex!tank.news.pipex.net!pipex!howland.reston.ans.net!newsfeed.internetmci.com!solaris.cc.vt.edu!news.new-york.net!actcom!news
From: Batikoff <aeb@shani.co.il>
Subject: Shaking all over the body
X-Nntp-Posting-Host: teleport901.shani.co.il
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Date: Thu, 15 Feb 1996 21:21:31 GMT
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Lines: 6

Hi, I'm 17 girl, few month ago I had a real hard headache, I tried to get of bed and I got 
real dizzy, I lied down to sleep and after some time I woke up and all my body was shaking, my 
parents took me to a specialist and he didn't know what is it.
if u know or u know any1 that dose waht happend to me please e-mail me at aeb@shani.co.il
please mail me I'm realy worried 10x bye.


From owner-diagnostics@net.bio.net Fri Feb 16 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!tube.news.pipex.net!pipex!dish.news.pipex.net!pipex!news00.sunet.se!sunic!news99.sunet.se!newsfeed.tip.net!peroni.ita.tip.net!i2unix!news
From: esposito@xtreme.it
Newsgroups: bionet.diagnostics
Subject: Fibrinogen
Date: Fri, 16 Feb 1996 08:32:50 GMT
Lines: 4
Message-ID: <4g0g16$g3j@news.iunet.it>
NNTP-Posting-Host: relay.xtreme.it
X-Newsreader: Forte Free Agent 1.0.82

I' m finding any recent article about correletion between fibrinogen
assay with method PT-derivated and assay with Clauss method;
please, help me!|


From owner-diagnostics@net.bio.net Fri Feb 16 22:00:00 1996
Path: biosci!OP1.UP.AC.ZA!KPAT1
From: KPAT1@OP1.UP.AC.ZA ("Fred Reyers")
Newsgroups: bionet.diagnostics
Subject: Re: Fibrinogen
Date: 17 Feb 1996 10:58:07 -0800
Organization: University of Pretoria
Lines: 31
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <7FB60D3A48@op1.up.ac.za>
NNTP-Posting-Host: net.bio.net

Hello "esposito"

On  Fri, 16 Feb 1996 08:32:50 GMT you wrote:

> I' m finding any recent article about correletion between fibrinogen
> assay with method PT-derivated and assay with Clauss method;
> please, help me!|

Try Journal of Veterinary Medicine (Series A), 1994, 41: 8, 587-598
"Measuring fibrinogen concentrations in healthy dogs etc
by Mischke R and Menzel D

Also Am J of Vet Research, 1981, 42: 1, 100-104
"Determination of plasma dfibrinogen in the horse
by Campbell MD et al

Regards

Fred
 
 Fred Reyers (Prof & Head: Section of Clinical Pathology)
Department of Medicine
Faculty of Veterinary Science
P.Bag X04, ONDERSTEPOORT, 0110,
Rep. of South Africa
Tel: 27-12-529 8288
FAX: 27-12-529 8308 or 27-12-344 2107 (Home)

                       "The great tradgedies of science
            are the slaying of beautiful hypotheses by ugly facts"
                              (Huxley)

From owner-diagnostics@net.bio.net Fri Feb 16 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!Austria.EU.net!newsfeed.ACO.net!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!zrz.TU-Berlin.DE!news
From: Bernhard Voslamber <Bernard@station1.fb12.tu-berlin.de>
Newsgroups: bionet.diagnostics
Subject: HELP: Literature Research
Date: Sat, 17 Feb 1996 22:16:15 +0100
Organization: TU-Berlin
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Dear Newsreader,

I'm searching for literature about the newest immunhistochemical markers 
for keratocysts.
I'm writing a studie about this theme and searching desperately for 
month.

Please E-Mail me answers at following adress: 
Bernard@.station1.fb12.tu-berlin.de

Great thanks for answers.

		Kerstin Schubert

From owner-diagnostics@net.bio.net Sat Feb 17 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!library.ucla.edu!unixg.ubc.ca!news.bc.net!rover.ucs.ualberta.ca!orion.supernet.ab.ca!usenet
From: John/Ann Crockett <crockett@supernet.ab.ca>
Newsgroups: bionet.diagnostics
Subject: G.I.Tract
Date: Sun, 18 Feb 1996 08:46:20 -0800
Organization: Alberta Supernet Inc
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Message-ID: <312757DC.73E3@supernet.ab.ca>
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A girl I work with has a five year old daughter who has been sick since
she was born with problems such as food allergies, migraines, seizures,
heart palpitations, decrease in weight, iron deficiency ,high fevers,
colicky, and major bloating.  Some times her abdomen is so distended
she looks like an Ethopian baby.  Her doctors and referring specialists
have no clue as to what is going on.  She now has plus plus elevated
eosiniphiles in her stools  and are treating her with steroids. The doctors
think that she might have eosinophilic gastroenteropathy but is very rare
in kids of her age not to mention that this condition is very rare period.
The questionable treatment is to hack out large amounts of her bowels.
Does anyone have or know anything about this disease or treatment or
any ideas as to what could be wrong with her.  Any help would be greatly
appreciated.  Thanks.
Ann Crockett

From owner-diagnostics@net.bio.net Sun Feb 18 22:00:00 1996
Newsgroups: bionet.diagnostics
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!news.sprintlink.net!news1!ind-009-237-108
From: mai@iquest.net (Patrick Croft)
Subject: Centrifuge Available
X-Nntp-Posting-Host: ind-003-236-130.iquest.net
Message-ID: <Dn1J99.IG5@iquest.net>
Keywords: Centrifuge
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Date: Mon, 19 Feb 1996 20:58:12 GMT
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Have 16 new BIOTOPE model Eclipse ICA including internal cpu, 1.44Fdd,  printer, etc.
Built in 1991-1992.  Also,  12,500 cassette cases, 95,000 pipettees, 3,600 blood modules,  650 spinner cups.
Approximately 16 additional units in various stages for spare parts, etc.  Would like to sell
as a lot, but consider offers for any portion.  These are blood analyzers of high quality, and
we hope someone will find them a home!

                       Milo Associates, Inc.    Indianapolis, IN    USA
          Telephone:(317)257-6811    Fax: (317)257-1590       email: mai@iquest.net




From owner-diagnostics@net.bio.net Mon Feb 19 22:00:00 1996
Path: biosci!daresbury!yama.mcc.ac.uk!peer-news.britain.eu.net!tank.news.pipex.net!pipex!newsfeed.internetmci.com!cdc2.cdc.net!news1.cris.com!news
From: wick@cris.com
Newsgroups: bionet.diagnostics
Subject: How do I contact NCCL???
Date: 20 Feb 1996 21:22:11 GMT
Organization: Molecular Biology Resources, Inc.
Lines: 16
Message-ID: <4gde23$2st@spectator.cris.com>
NNTP-Posting-Host: crc3-fddi.cris.com
X-Newsreader: AIR News 3.X (SPRY, Inc.)

Can anyone tell me how to contact the National Committee for Clinical Laboratory Standards. I am interested in getting a copy of 
"Evaluation of precision performance of clinical chemistry devices. Code EP5-T2.

Thanks
Jim Wick

E-mail:		wick@cris.com		
Phone:		414-871-7199
FAX:		414-871-1273
Snail Mail:	5520 W. Burleigh St.
		Milwaukee, WI 53210






From owner-diagnostics@net.bio.net Mon Feb 19 22:00:00 1996
Path: biosci!MAGI.COM!eja
From: eja@MAGI.COM
Newsgroups: bionet.diagnostics
Subject: Info needed for prairie weasel
Date: 19 Feb 1996 18:08:00 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 12
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199602200205.VAA29342@infoweb.magi.com>
NNTP-Posting-Host: net.bio.net

I am a grade 10 student and I would like to know if anyone has any
information about the "prairie long tailed weasel".  I have been assigned a
project in science class about endangered species and was assigned the
prairie long tailed weasel.  I have not been able to find information in
local librairies and I would appreciate any help you can give me.

Please feel free to pass this request to anyone who might be able to help me.

Thank you very much.

Andrew


From owner-diagnostics@net.bio.net Mon Feb 19 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!gossip.pyramid.com!news.sedona.net!usenet
From: Gene Rathbun <rathbun@sedona.net>
Newsgroups: bionet.diagnostics
Subject: QE/Statistician Position
Date: 19 Feb 1996 18:32:14 GMT
Organization: Sedona Internet Services, Inc.
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Quality Engineer (aka Statistician)

We have a statistician/QE/QA position open in the Medical 
Device/Instrumentation/Diagnostics/ Equipment industry in the San 
Francisco Bay Area.  The perfect candidate will have:

*  3-5 years experience,
*  at least a BS/MS in math or chemistry, with a MS or Ph.D. in math or 
statistics preferred,
*  strong computer & statistics experience & background,
*  good organizational skills,
*  outstanding written & verbal communications skills,
*  knowledge of GMP & ISO-9000.

If this position is yours, you will provide statistical support to & 
interface with all departments 
(manufacturing/R&D/electronics/biochemistry) & levels of staff; develop 
statistics-based specifications & procedures; conduct training seminars 
in statistics & be the company "Guru"; develop & validate computer 
programs for data collection & analysis; coordinate company validation 
program; conduct internal & external quality audits; write documentation 
& justifications.


If you have an interest in this or other opportunities, please mail or 
FAX your CV/resume to RS&A to the attention of Gene Rathbun, Sr. Partner. 
All correspondence is held in strict confidence.


Rathbun, Sapir & Associates
P.O. Box 2337  Sedona, AZ 86339-2337 * USA
(520) 284-3360 Office  (520)284-3361 FAX
E-mail: rathbun@ sedona.net



From owner-diagnostics@net.bio.net Tue Feb 20 22:00:00 1996
Path: biosci!COMPUSERVE.COM!100416.735
From: 100416.735@COMPUSERVE.COM (The Diagnostics Club)
Newsgroups: bionet.diagnostics
Subject: Meeting notification
Date: 21 Feb 1996 07:00:33 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 45
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <960221145615_100416.735_BHG133-3@CompuServe.COM>
NNTP-Posting-Host: net.bio.net

	GENETIC DIAGNOSTICS: FORWARD WITH FORESIGHT

The UK Technology Foresight Health and Life Sciences Panel reviewed long-term
trends in science, health care, health-related industry, and non-medical
applications of the life sciences, and assessed the principal opportunities and
challenges. One of the priority areas in the recommendations from this Panel was
diagnostic applications of molecular biology.

The Diagnostics Club is holding a Workshop on Monday 15th April 1996 at Hulme
Hall, University of Manchester, UK to examine and flesh-out what are the UK's
R&D and commercial strengths and weaknesses in genetic-based diagnostics; and
what can be done to address the weaknesses and utilize the strengths.

The Workshop will take the format of introductory speakers prior to the
delegates dividing into four (preselected) interest discussion groups, namely:
Sample preparation for genetic testing, Automation, Multi-mutation detection,
and Intellectual Property Rights. When reconvened, the Group leaders and
Raconteurs will present the conclusions developed by their (own) groups.  It is
anticipated that the discussion groups will come up with a list of future
activities to drive forward the UK genetics-based diagnostics industry to the
benefit of all.

OUTLINE PROGRAMME

Findings of the Foresight Programme in Genetic Diagnostics
      Prof Mark Ferguson, School of Biological Sciences, University of
Manchester
Medical genetics - development of services in the NHS and European healthcare
services.
     Prof Rodney Harris, Dept of Medical Genetics, University of Manchester
The use of DNA testing in diagnosis of infectious diseases
     Prof James Burnie, Dept of Pathological Sciences, University of Manchester
Break into four discussion groups
	Sample preparation for genetic testing
Automation
	Multi-mutation detection
	Intellectual Property Rights
Combined reporting by workshop chairmen and raconteurs, followed by general
discussion

For further information contact:
Dr Valerie Owen, Diagnostics Club Manager, The Diagnostics Club, 1 Otter Close,
Bletchley, Milton Keynes MK3 7QP, UK, 
tel: +44 1908 647417, fax: +44 1908 271612, email: 100416.735@compuserve.com


From owner-diagnostics@net.bio.net Tue Feb 20 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!usc!newshub.cts.com!usenet
From: b3748@cts.com (Bryan Kiehl)
Newsgroups: bionet.diagnostics
Subject: Re: How do I contact NCCL???
Date: Wed, 21 Feb 1996 17:47:23 GMT
Organization: CTS Network Services
Lines: 31
Message-ID: <4gflm4$l0k@news2.cts.com>
References: <4gde23$2st@spectator.cris.com>
NNTP-Posting-Host: kiehl.cts.com

NCCLS Address is:

940 West Valley Road
Suite 1400
Wayne, PA  19087-1898
(610)688-0100
(610)688-0700 (FAX)

wick@cris.com wrote:

>Can anyone tell me how to contact the National Committee for Clinical Laboratory Standards. I am interested in getting a copy of 
>"Evaluation of precision performance of clinical chemistry devices. Code EP5-T2.

>Thanks
>Jim Wick

>E-mail:		wick@cris.com		
>Phone:		414-871-7199
>FAX:		414-871-1273
>Snail Mail:	5520 W. Burleigh St.
>		Milwaukee, WI 53210






Bryan Kiehl
b3748@cts.com
San Diego, CA


From owner-diagnostics@net.bio.net Tue Feb 20 22:00:00 1996
Path: biosci!bcm.tmc.edu!cs.utexas.edu!natinst.com!news-relay.us.dell.com!swrinde!howland.reston.ans.net!quagga.ru.ac.za!uct.ac.za!ovisun.ovi.ac.za!amazon
From: venessa@moon.ovi.ac.za (Venessa Scott)
Newsgroups: bionet.diagnostics,bionet.general,bionet.immunology,bionet.virology
Subject: Cysticercosis
Date: 13 Feb 1996 13:19:06 GMT
Organization: Onderstepoort Veterinary Institute
Lines: 18
Message-ID: <4fq34a$rc8@ovisun.ovi.ac.za>
NNTP-Posting-Host: .ovi.ac.za
X-Newsreader: News Xpress Version 1.0 Beta #4
Xref: biosci bionet.diagnostics:656 bionet.general:20012 bionet.immunology:7848 bionet.virology:6347

Greetings

We are in the process of reviewing cysticercosis (bovine) world wide.

Can anyone please provide recent references to biology (susceptibility,
immune response, migration and cyst evolution and degeneration(?)
over time) incidence, diagnosis and economics.

Any and all assistance greatly appreciated.


Sincere regards.


Anthonie Snijders
Onderstepoort Veterinary Institute

Please reply by e-mail to Venessa Scott (Venessa@moon.ovi.ac.za)

From owner-diagnostics@net.bio.net Wed Feb 21 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!news2.acs.oakland.edu!news1.cris.com!news
From: "Y. Thakore" <kyteassoc@aol.com>
Newsgroups: bionet.diagnostics
Subject: Biosensors in Diagnostics
Date: 22 Feb 1996 12:41:12 GMT
Organization: Kyte Associates, Inc.
Lines: 15
Message-ID: <4gho98$fo1@spectator.cris.com>
NNTP-Posting-Host: cnc117034.concentric.net
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.2 (Windows; U; 16bit)

Hi!

I am looking for examples of commercial or near-commercial 
applications of biosensors in diagnostics for an article. 
Pariculary the miniaturized sensors as in Field Effect 
Transistors or similar types of devices. 

I'll appreciate your help.

Thanks in advance.

Yatin B. Thakrore
Kyte Associates, Inc., NJ , USA Tel: 908-390-7435
Fax: 908-651-8557; e-mail: kyteassoc@aol.com


From owner-diagnostics@net.bio.net Wed Feb 21 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Re: Biosensors in Diagnostics
Date: 22 Feb 1996 05:45:09 -0800
Organization: Scottish Agricultural Science Agency
Lines: 36
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9602221341.aa07565@jura.sasa.gov.uk>
Reply-To: odonnell@sasa.gov.uk
NNTP-Posting-Host: net.bio.net

On 22 Feb 96 at 12:41, Y. Thakore wrote:

> Hi!
> 
> I am looking for examples of commercial or near-commercial 
> applications of biosensors in diagnostics for an article. 
> Pariculary the miniaturized sensors as in Field Effect 
> Transistors or similar types of devices. 
> 
> I'll appreciate your help.
> 
There is a conference on 'Biochip Array Technologies' being held on March 18,
 1996 in Marina del Rey, California.It includes a paper from Beckman 
Instruments on 'Design and use of disposable genosensors for the detection 
of Ras oncogene mutations' which sound like it could be roughly what you're
 looking for.

The organisers are:

IBC USA Conferences inc.
225 Turnpike Road
Southborough
MA 01772-1749
USA

Tel. (508) 481-6400

Fax (508) 481-7911

Hope this is of some help,

Kevin
Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Wed Feb 21 22:00:00 1996
Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news
From: Fei Wang <102722.3070@CompuServe.COM>
Newsgroups: bionet.diagnostics
Subject: rare disease
Date: 22 Feb 1996 15:34:06 GMT
Organization: A patient father
Lines: 7
Message-ID: <4gi2de$p8i$1@mhade.production.compuserve.com>

A Chinese boy with an almost 3 year history of gait abnormality 
and tentative diagnosis of leukodystrophy of unknown etiology.
More detail see my home page:
http://ourworld.compuserve.com/homepages/wang/

-- 
Fei Wang

From owner-diagnostics@net.bio.net Thu Feb 22 22:00:00 1996
Newsgroups: bionet.diagnostics
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!nntp-hub2.barrnet.net!snooze.ser.bbnplanet.com!es.dupont.com!esds01.es.dupont.com!piersojf.gg.dupont.com!user
From: piersonperry@csoc.dnet.dupont.com (Jim Pierson-Perry)
Subject: Re: How do I contact NCCL???
Message-ID: <piersonperry-2202961225090001@piersojf.gg.dupont.com>
Sender: news@es.dupont.com (USENET News System)
Nntp-Posting-Host: piersojf.gg.dupont.com
Organization: DuPont Medical Products
References: <4gde23$2st@spectator.cris.com>
Date: Thu, 22 Feb 1996 17:19:08 GMT
Lines: 26

In article <4gde23$2st@spectator.cris.com>, wick@cris.com wrote:

>Can anyone tell me how to contact the National Committee for Clinical
Laboratory Standards. I am interested in getting a copy of 
>"Evaluation of precision performance of clinical chemistry devices. Code
EP5-T2.
>
NCCLS
940 West Valley Road, Suite 1400
Wayne, PA  19087-1898

Order by phone: 610/688-1100
Order by fax: 610/688-6400

Evaluation protocol EP5-T2 costs $25 (members), $85 (nonmembers)
It is also available in an "Evaluation Protocols Specialty Collection"
along with EP6 (Linearity), EP7 (Interferences), EP9 (Method Comparison),
 and EP10 (Eval of Quantitative Clin Lab Methods), and GP10(Clinical
Accuracy by ROC). This costs $125 (members), $250 (nonmembers)


/////////////////////////////////////////////////////////////////////
Content and bias are mine alone and do not represent the DuPont Company

"Even chaos has a pattern..."
/////////////////////////////////////////////////////////////////////

From owner-diagnostics@net.bio.net Fri Feb 23 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!usc!newshub.cts.com!usenet
From: b3748@cts.com (Bryan Kiehl)
Newsgroups: bionet.diagnostics
Subject: EBNA Protein
Date: Sat, 24 Feb 1996 21:40:55 GMT
Organization: CTS Network Services
Lines: 6
Message-ID: <4go0h4$52o@news2.cts.com>
NNTP-Posting-Host: kiehl.cts.com

I'm looking for EBNA 1 and 2 recombinant proteins to use in a
commercial assay. Any suggestions?
Bryan Kiehl
b3748@cts.com
San Diego, CA


From owner-diagnostics@net.bio.net Sun Feb 25 22:00:00 1996
Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-lyon1.fr!rockendo.univ-lyon1.fr!pcomm
From: pcomm@rabelais.univ-lyon1.fr (Pierre Commercon)
Newsgroups: bionet.diagnostics
Subject: Antibody against Foetal Hemoglobin (for ELISA  test)
Date: Mon, 26 Feb 1996 14:14:40
Organization: Universite Lyon1 Pharmacie
Lines: 10
Message-ID: <pcomm.240.000E3F00@rabelais.univ-lyon1.fr>
NNTP-Posting-Host: rockendo.univ-lyon1.fr
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Hello

I am looking for specific Antibody against Foetal Hemoglobin,
it is for an Elisa Test......

Where can I found a saler of this Ab.

Merci



From owner-diagnostics@net.bio.net Sun Feb 25 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!gatech!newsjunkie.ans.net!newsfeeds.ans.net!news.nmcnj.ico.att.net!niamh.indigo.ie!usenet
From: Barney Henry <bhenry@indigo.ie>
Newsgroups: bionet.diagnostics
Subject: Biotrin International WWW site
Date: Mon, 26 Feb 1996 10:20:02 +0000
Organization: Biotrin International Ltd.
Lines: 15
Message-ID: <31318952.36C5@indigo.ie>
NNTP-Posting-Host: dublin-ts10-207.indigo.ie
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 2.0 (Win16; I)

Just a quick note for anybody who may have been trying to access the 
www site of Biotrin or attempting to send email to us over the last 2 
weeks.

We have changed our internet provider and our new address is

		http://www.biotrin.ie/biotrin

Regards,

Barney Henry
MIS Manager
Biotrin International.

bhenry@biotrin.ie

From owner-diagnostics@net.bio.net Sun Feb 25 22:00:00 1996
Path: biosci!data-transport.com!scott_jokerst
From: scott_jokerst@data-transport.com (R. Scott Jokerst)
Newsgroups: bionet.diagnostics
Subject: Re: Biotrin International WWW site
Date: 26 Feb 1996 08:35:03 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 40
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <ad578dd8050210045e87@[204.188.159.35]>
NNTP-Posting-Host: net.bio.net

Hello Barney,

Perhaps it may be a good time to review your entry at Biological Data
Transport's site (Biotech Registry).  Please be sure to change/add any of
the contact information for which you would like your WWW audience to be
aware.  You can find us again at:

        http://www.data-transport.com

You may notice several additions and enhancements to our site and services
since you last visited.  In particular, we have launched services making it
a snap for your customers using our site to find information regarding your
products and services at the time they are considering their use.

Looking forward to hearing from you,

Scott
***************

At 2:20 AM 2/26/96, Barney Henry wrote:
[snip]
>
>We have changed our internet provider and our new address is
>
>                http://www.biotrin.ie/biotrin
>
>Regards,
>
>Barney Henry
>MIS Manager
>Biotrin International.
>
>bhenry@biotrin.ie

  ---> R. Scott Jokerst            scott_jokerst@data-transport.com  --->
--->   Biological Data Transport   http://www.data-transport.com       --->
 --->  510-648-8229                510-648-8279 (FAX)               --->
                  



From owner-diagnostics@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!PO.IIJNET.OR.JP!srldx
From: srldx@PO.IIJNET.OR.JP (Hajime Sugisaki)
Newsgroups: bionet.diagnostics
Subject: Endotoxin and endotoxin antibody
Date: 26 Feb 1996 16:54:58 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199602270052.JAA22399@sh0.po.iijnet.or.jp>
NNTP-Posting-Host: net.bio.net

I appreciate it indeed if somebody advise me a possible source from which I
can procure antibody (monoclonal) to endotoxin of gram negative bacilli. My
lab. colleagues have their idea to prepare a rapid test to quantfy the
material. It is more convenient if there is a commercial kit available. 
Tel: (Japan)-426-48-4017, Fax: (Japan)-426-48-4087, E-Mail:
srldx@iijnet.or.jp, Address: 51 Komiya Hachioji Tokyo 192 Japan, SRL, Inc.
Intellectual Property and Licensing, H. Sugisaki


From owner-diagnostics@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news-feed.iguide.com!uunet!in2.uu.net!news.iij.ad.jp!rim.or.jp!st.rim.or.jp!news
From: tishi@st.rim.or.jp (Tatsuzo Ishigami)
Newsgroups: bionet.diagnostics
Subject: Re: How do I contact NCCL???
Date: 27 Feb 1996 01:33:13 GMT
Organization: Rim-Net User
Lines: 19
Message-ID: <4gtn0p$i9v@news.st.rim.or.jp>
References: <4gde23$2st@spectator.cris.com>
NNTP-Posting-Host: ppp731.st.rim.or.jp
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
X-Posting-Software: WSSoup 0.81 (UNREGISTERED)

In article <4gde23$2st@spectator.cris.com>, wick@cris.com wrote:
>Can anyone tell me how to contact the National Committee for Clinical
>Laboratory Standards. I am interested in getting a copy of 
>"Evaluation of precision performance of clinical chemistry devices. 
>Code EP5-T2.

Hi there!

This NCCLS Approved Guideline is distributing to the NCCLS member.
You can purchase it directly from NCCLS.

National Committee for Clinical Laboratory Standards(NCCLS)

940 West Valley Road, Ste 1400, Wayne, PA 19087-1898, U.S.A.
Phone: 610-688-0100  Fax: 610-688-0700

Tatsuzo Ishigami, Ph.D.
SRL, Inc., Tokyo, Japan
E-mail: tat@cup.com

From owner-diagnostics@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!bangslabs.com!Leigh
From: Leigh@bangslabs.com ("Leigh")
Newsgroups: bionet.diagnostics
Subject: Latex in Diagnostics Course
Date: 27 Feb 1996 15:13:22 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 30
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <m0trYWj-00032PC@uucp.iquest.net>
NNTP-Posting-Host: net.bio.net

                      Subject:                              Time:  15:36
  OFFICE MEMO         Latex in Diagnostics Course           Date:  96/01/10
Announcing the 7th Annual Latex Course: Diagnostic Applications of Latex
Technology
Very briefly, here are the details: 
Content: Theory and practical details of coating and using microspheres in tests
and assays (& other biomed applications)
Faculty: 9 internationally distinguished researchers and consultants in the
field.
Dates: April 17-19, 1996 in Princeton, NJ and 
          April 22-24, 1996 in Paris (France). 
Cost: $895 (including most meals)
Registration Deadline: March 15, 1996  (After this date, call for possible late
registration.) 

A recent message on this forum said it's OK to post notices about conferences,
so I hope I'm not violating the moral code for this group, but I also don't have
the energy to get a friend to post this so we can pretend that it came from
outside. I really think it is pertinent for this group.

If you have any interest, please ask for "Latex Course brochure" and give postal
address where we can send it.
(Complete details and registration forms are also at our web site: 
www.bangslabs.com/blab)

Leigh Bangs, aka "The Particle Doctor";  leigh@bangslabs.com 
Bangs Laboratories, Inc., 979 Keystone Way, Carmel, IN 46032-2823 USA
Tel: 317-844-7176  Fax: 317-575-8801
"The Microsphere Zone"  [Web Home Page: http://www.bangslabs.com/blab] 


From owner-diagnostics@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news-feed.iguide.com!uunet!in1.uu.net!news-relay.eworld.com!news-relay.eworld.com!not-for-mail
From: easelabs@eworld.com (EASELABS)
Newsgroups: bionet.diagnostics
Subject: Re: Biosensors in Diagnostics
Date: 27 Feb 1996 14:16:56 -0800
Organization: eWorld
Lines: 33
Sender: root@news-relay.eworld.com
Message-ID: <4gvvso$aff@hp5.online.apple.com>
References: <4gho98$fo1@spectator.cris.com>
Reply-To: easelabs@eworld.com (EASELABS)
NNTP-Posting-Host: hp5.online.apple.com


The FET technology incorporation into biosensors is a fascinating new
development, as are the more advanced ISFET applications.  Your request
for information on the practical uses of these technologies in diagnostics
may be facilitated by the recent article in Clinical Chemistry entitled: 
Immunosensors:  technology and opportunities in laboratory medicine. (Clin
Chem 42:2. pp.193-209)

Good luck, and please let us know where your article will be published.

B

RE:
Subject: Biosensors in Diagnostics
From: "Y. Thakore" <kyteassoc@aol.com>
Date: 22 Feb 1996 12:41:12 GMT
Message-ID: <4gho98$fo1@spectator.cris.com>

Hi!

I am looking for examples of commercial or near-commercial 
applications of biosensors in diagnostics for an article. 
Pariculary the miniaturized sensors as in Field Effect 
Transistors or similar types of devices. 

I'll appreciate your help.

Thanks in advance.

Yatin B. Thakrore
Kyte Associates, Inc., NJ , USA Tel: 908-390-7435
Fax: 908-651-8557; e-mail: kyteassoc@aol.com


From owner-diagnostics@net.bio.net Mon Feb 26 22:00:00 1996
Path: biosci!AGERI.SCI.EG!idl
From: idl@AGERI.SCI.EG
Newsgroups: bionet.diagnostics
Subject: Re: Endotoxin and endotoxin antibody
Date: 26 Feb 1996 22:45:35 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 16
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.3.85.9602270837.A5938-0100000@ageri.sci.eg>
References: <199602270052.JAA22399@sh0.po.iijnet.or.jp>
NNTP-Posting-Host: net.bio.net

it may be useful to you if you contact with the American Type Culture 
Collection (ATCC), Maryland,USA.

On 26 Feb 1996, Hajime Sugisaki wrote:

> I appreciate it indeed if somebody advise me a possible source from which I
> can procure antibody (monoclonal) to endotoxin of gram negative bacilli. My
> lab. colleagues have their idea to prepare a rapid test to quantfy the
> material. It is more convenient if there is a commercial kit available. 
> Tel: (Japan)-426-48-4017, Fax: (Japan)-426-48-4087, E-Mail:
> srldx@iijnet.or.jp, Address: 51 Komiya Hachioji Tokyo 192 Japan, SRL, Inc.
> Intellectual Property and Licensing, H. Sugisaki
> 
> 
> 


From owner-diagnostics@net.bio.net Tue Feb 27 22:00:00 1996
Path: biosci!AGERI.SCI.EG!idl
From: idl@AGERI.SCI.EG
Newsgroups: bionet.diagnostics
Subject: (none)
Date: 28 Feb 1996 08:01:03 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.3.85.9602281707.A7680-0100000@ageri.sci.eg>
NNTP-Posting-Host: net.bio.net

for all netters:
	Could anyone sned me more information about applying Ultra sonic on 	
nematodes. By the way my lab has sonic dismemberator "Model 550" from 
Fisher Scientific Company.Thank you in advance.

Abd El-Nasser 
Agricultural Genetic Engineering Research Institute(AGERI)
Egypt
	



From owner-diagnostics@net.bio.net Tue Feb 27 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!vixen.cso.uiuc.edu!rhf.bradley.edu!sunca.ncaur.gov!usenet
From: Vicki Thompson <thompsvs@ncaur1.ncaur.gov>
Newsgroups: bionet.diagnostics
Subject: fiber-optic sensors companies
Date: 28 Feb 1996 14:56:56 GMT
Organization: USDA
Lines: 7
Message-ID: <4h1qfo$btd@sunca.ncaur.gov>
NNTP-Posting-Host: novix.ncaur.gov
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.2N (Windows; I; 16bit)

Are there any companies currently working to develop commercial 
fiber-optic biosensors?  I am particularly interested in those using 
antibodies as the recognition element, but other applications would also 
be of interest. Thanks

Vicki Thompson 


From owner-diagnostics@net.bio.net Tue Feb 27 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.iij.ad.jp!rim.or.jp!st.rim.or.jp!news
From: tishi@st.rim.or.jp (Tatsuzo Ishigami)
Newsgroups: bionet.diagnostics
Subject: Re: How do I contact NCCL???
Date: 28 Feb 1996 10:36:20 GMT
Organization: Rim-Net User
Lines: 19
Message-ID: <4h1b74$qre@news.st.rim.or.jp>
References: <4gde23$2st@spectator.cris.com>
NNTP-Posting-Host: ppp749.st.rim.or.jp
Mime-Version: 1.0
Content-Type: text/plain; charset=US-ASCII
X-Posting-Software: WSSoup 0.81 (UNREGISTERED)

In article <4gde23$2st@spectator.cris.com>, wick@cris.com wrote:
>Can anyone tell me how to contact the National Committee for Clinical
>Laboratory Standards. I am interested in getting a copy of 
>"Evaluation of precision performance of clinical chemistry devices. 
>Code EP5-T2.

Hi there!

This NCCLS Approved Guideline is distributing to the NCCLS member.
You can purchase it directly from NCCLS.

National Committee for Clinical Laboratory Standards(NCCLS)

940 West Valley Road, Ste 1400, Wayne, PA 19087-1898, U.S.A.
Phone: 610-688-0100  Fax: 610-688-0700

Tatsuzo Ishigami, Ph.D.
SRL, Inc., Tokyo, Japan
E-mail: tat@cup.com

From owner-diagnostics@net.bio.net Thu Feb 29 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: vigfrid.ness@veths.no (Vigfrid Ness)
Newsgroups: bionet.diagnostics
Subject: Re: WWW address
Date: 1 Mar 1996 15:01:37 -0000
Lines: 28
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4h73gh$pkf@mserv1.dl.ac.uk>
X-Sender: nmhness@samson.veths.no
Original-To: diagnost@dl.ac.uk

Dynal is http://www.telepost.no/dynal


>Could you please let me know the WWW address of Dynal?
>Thanks,
>
>Christian Mouton
>Groupe de Recherche en Ecologie Buccale
>Faculte de medecine dentaire, Universite Laval
>
>Quebec (Quebec) G1K 7P4  CANADA
>tel. (418)656-5872;  fax. (418)656-2861
>
>
>
>
>
>
>
Vigfrid Ness
Dynal Microbiology R&D
DYNAL A.S.
Norway

E.mail: vigfrid.ness@veths.no
Phone: +47-22-96-48-08
Fax: +47-22-96-48-14


