From owner-diagnostics@net.bio.net Sat Mar 02 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!gatech!newsfeed.internetmci.com!news.zeitgeist.net!bdt.com!usenet
From: "R. Scott Jokerst" <scott_jokerst@data-transport.com>
Newsgroups: bionet.diagnostics
Subject: Biological Data Transport info
Date: Sat, 02 Mar 1996 18:21:28 -0800
Organization: Biological Data Transport
Lines: 30
Message-ID: <31390228.22E@data-transport.com>
Reply-To: scott_jokerst@data-transport.com
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CC: "R. Scott Jokerst" <scott_jokerst@data-transport.com>

I wanted to let you know about a service we offer to the life sciences 
community on the Web.  Our site is emerging as a great source for 
links to bioinformatics resources, company products and services 
information, etc.  We're becoming more comprehensive daily.  Our 
growth has been quite rapid.

We're at:       http://www.data-transport.com

The entire collection of product, services, and public information 
links integrated within our system may be found by searching at our 
site.  This allows you to FOCUS on just the information you need, 
reducing the effort needed to find what you want.

The site is exceptionally fast, and we have a track record of being up 
virtually all of the time.  Everything we offer at the site works, (we 
do not post services under construction) and we have more coming soon.

We are eager to receive feedback from you regarding how well the site 
serves you, and what you would like to see to help you be more 
productive.

Thanks ahead of time.  It's worth a look,

Scott
(P.S. I have cross posted to other lists, wanting to get the info out 
to you.)
-- 
   R. Scott Jokerst                   Biological Data Transport
   scott_jokerst@data-transport.com   510-648-8229
   http://www.data-transport.com      510-648-8279 (FAX)

From owner-diagnostics@net.bio.net Sat Mar 02 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!newsxfer2.itd.umich.edu!news.itd.umich.edu!umich.edu!jchristn
From: jchristn@umich.edu
Newsgroups: bionet.diagnostics
Subject: ELISA reagent suppliers help needed!
Date: Fri, 1 Mar 1996 15:28:07
Organization: University of Michigan
Lines: 17
Distribution: world
Message-ID: <jchristn.43.000F786B@umich.edu>
NNTP-Posting-Host: pm002-05.dialip.mich.net
Keywords: ELISA, immunoglobulin, substrate
X-Newsreader: Trumpet for Windows [Version 1.0 Rev A]

Our laboratory is developing ELISA assays for quatitating levels of human IgG 
subclasses. However, we need to know the specificity of our reagents with 
respect to IgG subclass allotype reactivity. Does anyone know of commercial 
suppliers for these reagents or how to go about getting the standards from the 
WHO?

Also, we recently had trouble with KPL laboratories refusing to replace a 
suspect batch of ELISA and western blot substrates that they sent us. Does 
anyone know of suppliers of comparable reagents to their 2-component TMB 
liguid substrate and their 1-component NBT/BCIP liquid substrate?

Please E-mail me directly because I do not have regular access to Usenet.

Thanks in advance,
Rob Christner
Igglab@opus.mco.edu
rchristner@opus.mco.edu

From owner-diagnostics@net.bio.net Sat Mar 02 22:00:00 1996
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.diagnostics
Subject: IMPORTANT - BIOSCI Fundraising Update!
Date: 3 Mar 1996 02:00:28 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 149
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199603031000.CAA12373@net.bio.net>
NNTP-Posting-Host: net.bio.net


I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to
bring you up to date on BIOSCI fundraising progress, a topic of
concern to your future use of this resource.  Thank you in advance for
taking the time to read this message carefully.

Last year we announced that BIOSCI was going to adopt the U.S. Public
Broadcasting System model to fund its operations after our DOE/NSF
grant runs out later this year.  Unlike PBS, we are not soliciting
contributions from users; we are only selling ads on our Web pages
solely to cover our operating costs.  Our goal is to seek sponsorships
until we build up an operating reserve of about $100,000 and then
cease further promotions until we need to build the reserve back up.
(The accountants among our readership will be familiar with the
problem of deferred revenue which we can not safely utilize until ads
have been displayed for a period of time.)  We have three sponsors to
date with a couple more pending.  The process is time-consuming,
however, and we need your help as explained further below.

Our operating costs consist of our network connection, phone lines,
hardware maintenance (we hope to have new and faster hardware soon!),
plus 0.7 FTE of salaries covering UNIX systems admin, technical
support, quality assurance, i.e., testing, of our system, and
administrative costs (such as the time it takes to actually
find/write/call potential sponsors and raise money!).  Although the
BIOSCI staff does get compensated for a portion of the work that they
do, this project has always received a lot of free after-hours and
"vacation" time labor, so we hope that no one will begrudge the time
that we do charge to the project to serve you.  All of the three
part-time staff members, Dave Mack, Julie Lawrence, and myself, have
full time day jobs and families in addition to working hard to keep
this service running for all of you.  Julie and Dave Mack are
subcontractors for BIOSCI; my time that is charged to the project
defrays a portion of my regular salary instead of adding to my income.

Besides having to relocate the project, we were very busy this last
year building new infrastructure such as our WWW hypermail interface
to the system.  This was released last December along with scores of
WAIS indices for the newsgroups.  Virtually everything is complete,
although we do continue to find and fix bugs (many through your
helpful feedback!).  We are still having some problems with our WAIS
indexing.  The archives continue to grow rapidly.  We are running over
100 indexes now versus three previously and any systems crashes cause
greater havoc with the indexing than before!  We are still working to
fix this as fast as our resources permit and appreciate your patience,
but we have been able to automate a lot of the infrastructure to
reduce labor as compared to past requirements.

We have also implemented new software to make moderation of
BIOSCI/bionet newsgroups much easier and combat the growing problem of
Internet junk mail and USENET "spamming."  About 20% of our groups are
now moderated, many of them by the BIOSCI staff!  This, for example,
made a major difference last year in the quality of content in our
EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial
concerns and recruiting firms are using **without charge** to recruit
candidates for positions in the biological sciences.

We are also now in a position to have sponsors for individual
newsgroups as you will have noticed if you have visited
http://www.bio.net/ and clicked on "Access the BIOSCI/bionet
newsgroups" recently.

So, how can you help??
----------------------

As noted above it can take a lot of time to contact potential sponsors
if I have to do it all myself.  Our request is quite simple.  You can
do two important things which will take very little time for you
individually.  

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can now post or reply to messages via your Web browser.
Your usage helps attract sponsors.  If you contact any of our
sponsors, please be sure to thank them for supporting BIOSCI.  It is
critical for them to get this feedback if they are to continue their
sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community.  If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.

Our hope is to quickly raise several large corporate/institutional
sponsors on our heavily-used WWW locations (some stats appended
below), and then end this sponsorship campaign so that our resources
can continue to be used for service provision, not fundraising.  Many
of our specialty newsgroup WWW archives are still used by small
communities of scientists (and they haven't been heavily promoted
yet).  While these may be valuable niche markets to some advertisers,
it will generate more labor and overhead having to find these
sponsors, fairly price the locations, and deal with lots of smaller
sponsorships than fewer mid-to large sponsors.  We are striving to
keep our operation as lean and efficient as possible since we are not
trying to make careers out of running BIOSCI.  We are trying if at all
possible to avoid the administrative overhead entailed with processing
lots of small payments to reach our fundraising goals.

I'd like to thank all of you for your help in advance. In helping us,
you are also helping yourselves, not only in keeping this resource
available for all of the both large and small research communities
that we serve, but also by alleviating the need for us to go back and
compete with researchers for tight grant dollars!  We promised NSF
when we were awarded the BIOSCI grant that we would carry out this
mission to make the service self-supporting.  With your help, we will
succeed in continuing BIOSCI's work into its second decade.  Thank you
very much!

				Sincerely,

				Dave Kristofferson
				BIOSCI/bionet Manager

				biosci-help@net.bio.net


A list of our prime WWW sponsorship locations follow.  Statistics are
for the four week period from 22 Jan. - 18 Feb. 1996 and usage
continues to grow.
----------------------------------------------------------------------

The overall BIOSCI WWW pages are currently visited by users from close
to 5000 unique computer hosts per week.  Web servers only log the
Internet computer/host name and frequently more than one individual
can connect to us from a particular host.

Main home page, http://www.bio.net, visited recently by about 2100
unique hosts per week

Main Newsgroups archives page, http://www.bio.net/archives.html,
visited recently by about 1200 Unique hosts per week

BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html,
visited recently by about 1000 unique hosts per week.

EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ 
and monthly header pages, visited recently by about 600 unique hosts
per week.

Address database search page, http://www.bio.net/addrsearch.html,
visited recently by about 450 unique hosts per week.

Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS-
REAGNTS/ and monthly header pages, visited recently by about 350
unique hosts per week.
----------------------------------------------------------------------

From owner-diagnostics@net.bio.net Sat Mar 02 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!in1.uu.net!gossip.pyramid.com!news.sedona.net!usenet
From: Gene Rathbun <rathbun@sedona.net>
Newsgroups: bionet.diagnostics
Subject: Aerosol Engineers
Date: 28 Feb 1996 11:44:51 GMT
Organization: Sedona Internet Services, Inc.
Lines: 33
Message-ID: <4h1f7j$e2a@labrat.sedona.net>
NNTP-Posting-Host: client30.sedona.net
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.2N (Windows; I; 16bit)

We have two positions open on the West Coast for engineers with Aerosol 
experience.  Our client company is involved in Powdered Drug Delivery 
Systems and have asked us to find a Manager and an entry level engineer 
with the following backgrounds:
Requirements:
*  Experience with Aerosol Science in Industry: Practical experience in 
Particle Size Measurements, Eelectrostatic Precipitation, Incineration, 
Spray Drying, etc.
*  Min. of BS, preferably in Chem. Engineering [OK if physics, 
engineering, sciences]; Could be a PhD with less than 4 yr. industrial 
experience.
*  Excellent verbal, documentation & team skills; good sense of humor.
*  Creative problem solving skills, excellent analytical abilities.
*  Experience in Formulations, Powder Processing, Powder 
Characterizations.

Should Have [any combination]:
>  Experience in pharmaceutical/pharmacology (Very Big Plus!)
>  Knowledge of statistics and Design of Experiments.
>  Knowledge of NDA & IND, and pre-approval inspections.
>  Experience with UV, HPLC and protein assays a plus.

If you have an interest in this or other opportunities, please mail or 
FAX your CV/resume to RS&A to the attention of Gene Rathbun, Sr. Partner. 
 All correspondence is held in strict confidence.

Please Post

P.O. Box 2337  Sedona, AZ 86339-2337 * USA
(520) 284-3360 Office  (520)284-3361 FAX
E-mail: rathbun@ sedona.net



From owner-diagnostics@net.bio.net Sat Mar 02 22:00:00 1996
Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!nntp.crl.com!newshub.cts.com!usenet
From: b3748@cts.com (Bryan Kiehl)
Newsgroups: bionet.diagnostics
Subject: Re: ELISA reagent suppliers help needed!
Date: Sun, 03 Mar 1996 20:59:02 GMT
Organization: CTS Network Services
Lines: 36
Message-ID: <4hd0s6$irs@news2.cts.com>
References: <jchristn.43.000F786B@umich.edu>
NNTP-Posting-Host: kiehl.cts.com
X-Newsreader: Forte Free Agent 1.0.82

I suspect there are no WHO standards. As far as supplies, there are
several. I suggest that you find a copy of the AACC convention
information. Most of the suppliers attend this meeting.

I think you will find most larger manufactures do make their own
material, but generally do not sell these. We have reagents that are
made for our products, but we would not be able to specify for your
product. You just take what we have, but it is reproducible.

Good Luck. If you had questions, give me a call. (619-592-9300, ext
309)

jchristn@umich.edu wrote:

>Our laboratory is developing ELISA assays for quatitating levels of human IgG 
>subclasses. However, we need to know the specificity of our reagents with 
>respect to IgG subclass allotype reactivity. Does anyone know of commercial 
>suppliers for these reagents or how to go about getting the standards from the 
>WHO?

>Also, we recently had trouble with KPL laboratories refusing to replace a 
>suspect batch of ELISA and western blot substrates that they sent us. Does 
>anyone know of suppliers of comparable reagents to their 2-component TMB 
>liguid substrate and their 1-component NBT/BCIP liquid substrate?

>Please E-mail me directly because I do not have regular access to Usenet.

>Thanks in advance,
>Rob Christner
>Igglab@opus.mco.edu
>rchristner@opus.mco.edu

Bryan Kiehl
b3748@cts.com
San Diego, CA


From owner-diagnostics@net.bio.net Sun Mar 03 22:00:00 1996
Path: biosci!cc.UManitoba.CA!fernand
From: fernand@cc.UManitoba.CA (Marcel Fernandez)
Newsgroups: bionet.diagnostics
Subject: unsubcribe
Date: 4 Mar 1996 14:23:02 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 3
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9603042220.AA01139@toliman.cc.umanitoba.ca>
NNTP-Posting-Host: net.bio.net

take me off you list

thanks

From owner-diagnostics@net.bio.net Sun Mar 03 22:00:00 1996
Path: biosci!agresearch.cri.nz!HartA
From: HartA@agresearch.cri.nz
Newsgroups: bionet.diagnostics
Subject: Saliva
Date: 4 Mar 1996 14:49:00 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01I1Z89RS3KM000AGH@INVERMAY.CRI.NZ>
NNTP-Posting-Host: net.bio.net


I want to collect human saliva samples. I vaguely recall that it is possible 
to get absorbent pads that can be sucked/ chewed and then removed from the 
subjects mouth. Does anyone know of a source of these? Would the cylindrical 
pads that dentists use to keep their patient's teeth dry be suitable?

Thanks,
                  Alan Hart

From owner-diagnostics@net.bio.net Sun Mar 03 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Conference: Diagnostics in Crop Production
Date: 4 Mar 1996 02:25:16 -0800
Organization: Scottish Agricultural Science Agency
Lines: 44
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9603041020.aa14086@jura.sasa.gov.uk>
Reply-To: odonnell@sasa.gov.uk
NNTP-Posting-Host: net.bio.net


DIAGNOSTICS IN CROP PRODUCTION

1-3 April 1996

University of Warwick, Coventry, UK

Session 1 The Scientific Basis of Diagnostic Techniques

Session 2 Diagnostics for Viruses, Phytoplasmas and GMOs

Session 3 Diagnostics for Fungal Plant Pathogens

Session 4 Diagnostics for Lower Fungi, Bacteria and Nematodes 

Session 5 Development of Diagnostic Methods for Agrochemicals

Session 6 Diagnostics in Crop Production

Session 7 Validation, Regulatory Impact and Commercial Development of 
Diagnostics

Session 8 Diagnostics: The Future


Registration and Accomodation Enquiries:

British Crop Protection Enterprises Ltd.

49 Downing St.
Farnham
Surrey
GU9 7PH
UK

Tel.  +44 1252 733072

Fax +44 1252 727194


Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Sun Mar 03 22:00:00 1996
Path: biosci!aol.com!MHughes77
From: MHughes77@aol.com
Newsgroups: bionet.diagnostics
Subject: TMB liquid substrates for ELISAs
Date: 4 Mar 1996 11:15:21 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <960304141223_437746753@emout06.mail.aol.com>
NNTP-Posting-Host: net.bio.net

Dear Diagnostics Newgroup Members:

A question was recently posted regarding alternate sources for two-part TMB
liquid substrates for ELISAs using HRP conjugates.  After enjoying the
convenience and reliability of one-part TMB substrates for a few years, I am
curious as to why anyone would stick to using two-part substrates.  Have any
of you found any differences in sensitivity or linearity between the two
types of TMB?  I know that some brands of one-part substrates may suffer from
a fairly short shelf life, but I have used two brands that last for over a
year under refrigeration, one of which also lasts at least six months without
refrigeration.  The latter comes two forms, to produce either a soluble
product or a precipitate, both of which work equally well.

Mark Hughes
MHughes77@aol.com

From owner-diagnostics@net.bio.net Tue Mar 05 22:00:00 1996
Path: biosci!UCS.ORST.EDU!postmanj
From: postmanj@UCS.ORST.EDU (Joseph Postman)
Newsgroups: bionet.diagnostics
Subject: Rhododendron Necrotic Ringspot Virus
Date: 6 Mar 1996 09:28:17 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 21
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.OSF.3.91.960306091407.28849A-100000@ucs.orst.edu>
References: <4hjkbb$d69@mserv1.dl.ac.uk>
NNTP-Posting-Host: net.bio.net


Is anyone aware of a diagnostic tool (serology?, bioassay?) for 
rhododendron necrotic ringspot virus?  It produces lovely brown concentric 
ringspots on mountain laurel (Kalmia latifolia) plants in our landscape.  
I had an inquiry about how best to detect or confirm the presence of this 
virus in suspect rhododendrons.  

Joseph

                     ***   Feast on Genetic Diversity   ***
 =============================================================================
       Joseph D. Postman        |       N ational             /
      -PLANT PATHOLOGIST-       |       C lonal             / \   Eat More
     postmanj@bcc.orst.edu      |       G ermplasm         /   \   Fruit!
       corjp@ars-grin.gov       |       R epository        \ _ /
 =============================================================================
	USDA/Agricultural Research Service, Corvallis, Oregon, USA
           http://www.ars-grin.gov/ars/PacWest/Corvallis/ncgr




From owner-diagnostics@net.bio.net Tue Mar 05 22:00:00 1996
Path: biosci!daresbury!not-for-mail
From: Pete Kwasowski <P.Kwasowski@surrey.ac.uk>
Newsgroups: bionet.diagnostics
Subject: Bovine Body Fluids
Date: 6 Mar 1996 09:02:35 -0000
Lines: 12
Sender: lpddist@mserv1.dl.ac.uk
Distribution: bionet
Message-ID: <4hjkbb$d69@mserv1.dl.ac.uk>
X-Sender: bss3pk@central.surrey.ac.uk
Original-To: diagnost@dl.ac.uk

Hi all,

Does any one out there know of a US source of fresh (frozen) bovine body
fluids, including serum, urine and bile?

Any pointers would be appreciated.

Many Thanks.


Pete


From owner-diagnostics@net.bio.net Tue Mar 05 22:00:00 1996
Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.exodus.net!news.aimnet.com!alpha.sky.net!news
From: "Gwen E. Sprague" <gsprague@sky.net>
Newsgroups: bionet.diagnostics
Subject: Digoxin
Date: 6 Mar 1996 03:52:46 GMT
Organization: SkyNET Online
Lines: 13
Message-ID: <4hj26e$gpk@alpha.sky.net>
NNTP-Posting-Host: ip163.sky.net
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.1N (Macintosh; I; 68K)
X-URL: news:bionet.diagnostics


I'm looking for a 2 part digoxin methodology that has an application for the Chem1. Does anyone 
have any ideas?

Gwen


<*><*><*><*><*><*><*><*><*><*><*><*>
        >>> Freelance Technogoddess <<<
  "I am NOT unemployed, I'm a consultant."
<*><*><*><*><*><*><*><*><*><*><*><*>



From owner-diagnostics@net.bio.net Wed Mar 06 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!alterdial.uu.net!not-for-mail
From: "J. Oprandy" <oprandy@luxnet.com>
Newsgroups: bionet.diagnostics
Subject: WWW site for Consultants, Contract labs, Service Providers
Date: 7 Mar 1996 15:32:56 GMT
Organization: LuxNet, Inc.
Lines: 31
Message-ID: <4hmvj8$2sl@alterdial.UU.NET>
NNTP-Posting-Host: pool031.max1.washington.dc.dynip.alter.net
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 1.22 (Windows; U; 16bit)


Please allow me take this opportunity to introduce you to our information 
service for the biomedical community.  LuxNet is the premier source of 
news and information for the biotechnology, pharmaceutical and medical 
device industries.  The site is located at:

http://www.luxnet.com

LuxNet has a Newsroom and a Travel Center that contain numerous 
hyperlinks.  The site also contains ConsultNet and LabNet, searchable 
databases of detailed information on consultants and contract 
laboratories as well as other service providers.  

For a limited time, consultants and contract labs can get a 3 MONTH 
LISTING FREE.  

I hope you find the site interesting and useful.  Thank you for your 
consideration.  

JJO

--------------------------------------------------------------------
LuxNet, Inc.                         http://www.luxnet.com

 "The premier source for information, consultants and contract labs 
     for the biotech, pharma and medical device industries."

FAX:  301-963-9822                   phone: 301-963-5051
--------------------------------------------------------------------



From owner-diagnostics@net.bio.net Wed Mar 06 22:00:00 1996
Path: biosci!sasa.gov.uk!odonnell
From: odonnell@sasa.gov.uk ("Kevin O'Donnell")
Newsgroups: bionet.diagnostics
Subject: Microsatellite probes
Date: 7 Mar 1996 06:43:12 -0800
Organization: Scottish Agricultural Science Agency
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <9603071439.aa22043@jura.sasa.gov.uk>
Reply-To: odonnell@sasa.gov.uk
NNTP-Posting-Host: net.bio.net

Can anyone suggest a supplier of microsatellite probes, ideally 
biotin labelled?

Thanks,

Kevin
  
Dr Kevin O'Donnell                           "Work as if you were in the early days
Diagnostics and Molecular Biology     of a better nation"
SASA                                                    - Alasdair Gray
Edinburgh

From owner-diagnostics@net.bio.net Thu Mar 07 22:00:00 1996
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!usenet
From: line.roed@kjemi.uio.no (Line R|ed)
Newsgroups: bionet.diagnostics
Subject: Primers for PCR?
Date: 8 Mar 1996 10:00:36 GMT
Organization: Universitet i Oslo
Lines: 18
Message-ID: <4hp0g4$l5f@ratatosk.uio.no>
NNTP-Posting-Host: pckjemi103.uio.no
Mime-Version: 1.0
Content-Type: Text/Plain; charset=US-ASCII
X-Newsreader: WinVN 0.99.7

Hi!

We are to do PCR on whole blood from dogs. We have no information on 
sequences, 
restriction maps or anything - all we want is to get a PCR product of 
reasonable
length (1000-2000 bp).

Is there anyone who could suggest what primers to use? We have considered 
using
random primers of ca. length 12 bp. Are there any other suggestions?

Please mail us directly: line.roed@kjemi.uio.no

Regards,

Irina Arsky and Line Roed


From owner-diagnostics@net.bio.net Fri Mar 08 22:00:00 1996
Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!news.sprintlink.net!ralph.vnet.net!lys.vnet.net!not-for-mail
From: allbell@lys.vnet.net (Al Bell)
Newsgroups: bionet.diagnostics
Subject: whonet
Date: 8 Mar 1996 18:53:45 -0500
Organization: Vnet Internet Access, Inc. - Charlotte, NC. (704) 374-0779
Lines: 21
Message-ID: <4hqha9$hl4@lys.vnet.net>
NNTP-Posting-Host: lys.vnet.net

Whonet:

I noticed while doing a Web search that someone requested information 
about Whonet, an international system for reporting the spread of 
communicable diseases, especially those caused by antibiotic-resistant 
microbes.

I'm now sure what the number is, but they guy in charge of the project is 
Dr. Thomas O'Brien, head of microbiology and Brigham & Women's Hospital 
in Boston. (Just dial 617-555-1212 information for the number.)

There's also a little information about Whonet at my Web site, at 

http://www.vnet.net/users/allbell/belljar.html.


-- 
   Al Bell's Bell Jar - http://www.vnet.net/users/allbell/belljar.html
          "I'm just crazy about it." - Sylvia Plath
    Featuring: "Terror at 30 Rock (or: The Peacock Had Fangs)"
           allbell@vnet.net   allbell@delphi.com

From owner-diagnostics@net.bio.net Wed Mar 13 22:00:00 1996
Path: biosci!PO.IIJNET.OR.JP!srldx
From: srldx@PO.IIJNET.OR.JP (Hajime Sugisaki)
Newsgroups: bionet.diagnostics
Subject: Saliva diagnostic kit
Date: 13 Mar 1996 17:43:16 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 15
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199603140140.KAA03995@sh0.po.iijnet.or.jp>
NNTP-Posting-Host: net.bio.net

Dear netters, I really appreciate if somebody inform me of
diagnostic/pharmaceutical makers supplying  a diagnostic kit to detect
antibody (viral and bacterial antibodies) in patient's saliva. Our
laboratory received the increasing demand from dental offices. Thank you in
advance.
H. Sugisaki
Section Chief
Intellectual Property and Licensing Dept.
Reagent Production Dept. (concurrent)
SRL, Inc.
Tel: (Japan)-426-48-4017
Fax: (Japan)-426-48-4087
E-Mail: srldx@po.iijnet.or.jp
http://www.iijnet.or.jp/SRL-CO/


From owner-diagnostics@net.bio.net Sun Mar 17 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in1.uu.net!sleepy.inch.com!usenet
From: metin@inch.com
Newsgroups: bionet.diagnostics
Subject: Hematopathology workshop at NYU
Date: Mon, 18 Mar 1996 07:29:28 GMT
Organization: The Internet Channel
Lines: 347
Message-ID: <4iisc9$qvq@sleepy.inch.com>
NNTP-Posting-Host: port272.dialup.inch.com
X-Newsreader: Forte Free Agent 1.0.82

2ND ANNUAL HEMATOPATHOLOGY WORKSHOP AT  NEW YORK UNIVERSITY  (May
7-10)

COURSE DESCRIPTION
This concentrated 3 1/2 day course is designed to provide a
thorough and in-depth review of hematologic and lymphoid
pathology by the leading experts in this field. Both histopatho-
logic criteria and differential diagnosis and data provided by
immunopathology, molecular biology, and cytogenetics will
be discussed. A set of Kodachrome slides will be provided,
illustrating the pathology of post-thymic T-cell lymphomas.

EDUCATIONAL OBJECTIVES
This course is intended for practicing pathologists, residents
and fellows in pathology to update them on recent develop-
ments and the latest diagnostic techniques in current problem
areas.

EXPECTED OUTCOME
It is expected that the participants will acquire a wider knowledge of
 hemato-lymphoid disorders-especially neoplastic, as
well as a definite sense for the morphologic criteria and the
additional specialized studies required for their diagnosis.
                                  
FACULTY

Course Director
Glauco Frizera, M.D.
Professor of Pathology and Director  of Hematopathology
NYU School of Medicine
New York, New York

Co-Directors
Robert W. McKenna, M.D.
Professor and Vice-Chairman
Department of Pathology
Southwestern Medical School
Dallas, Texas

Giorgio Inghirami, M.D.
Associate Professor of Pathology
and Director of Molecular Pathology
NYU School of Medicine
New York, New York

Special Guest Lecturer
Janet D. Rowley, M.D.
Blum-Riese Professor
Departments of Medicine and Molecular Cytogenetics and Cell Biology
University of Chicago School of Medicine
Chicago, Illinois

Guest Faculty
Clara D. Bloomfield, M.D.
Professor of Medicine
Chief, Division of Oncology
State University of New York at Buffalo
Head, Department of Medicine
Roswell Park Cancer Institute
Buffalo, New York

Jerome S. Burke, M.D.
Clinical Professor of Pathology
University of California School of Medicine
San Francisco California
Director of Surgical Pathology
Alta  Bates Medical Center
Berkeley, California

Kathy Foucar, M.D.
Professor of Pathology and Director of the Pathology Service
University of New Mexico Hospital
Albuquerque, New Mexico
 

Nancy L. Harris, M.D.
Associate Professor of Pathology and Director of Anatomic Pathology
Massachusetts General Hospital
Harvard Medical School
Boston, Massachusetts

Elaine s. Jaffe, M.D.
Chief, Hematopathology Section
Laboratory of Pathology
National Cancer Institute
Bethesda, Maryland

Daniel M. Knowles, M.D.,
Professor and Chairman of Pathology
The New York Hospital -Cornell Medical Center
New York, New York

Bharat N. Nathwani, M.D.
Professor of Pathology and Chief of Hematopathology
USC School of Medicine
Los Angeles, California

Sibrand Poppema, M.D.
Professor and Chairman of Pathology
University of Groningen and Academic Hospital
Groningen, The Netherlands

James W. Vardiman, M.D.
Professor of Pathology
Director of Hematology/Hematopathology
University of Chicago Hospital
Chicago, Illinois


NYU Faculty
Hideko Kamino, M.D.
Director of Dermatopathology
NYU Medical Center
New York, New York

Stefano Rosati, M.D.
Assistant Professor
Department of Pathology
NYU School of Medicine
New York, New York

Program Schedule
Tuesday
Normal Lymphoid Tissues:  Review
Nancy L. Harris, M.D.
 
Reactive Lymphadenopathies
Glauco Frizzera, M.D.
 
Atypical Lymphoproliferative Disorders
Glauco Frizzera, M.D.

Immunophenotypic Characterization of  Hematopoietic Malignancies
Giorgio Inghirami, M.D.
 
  
Molecular Genetic Basis of Lymphoid  Neoplasms
Giorgio Inghirami, M.D.

Post-Transplantation Lymphoproliferative  Disorders: Clinical and
Biologic Aspects I
Daniel M. Knowles, M.D.
 
Benign and Malignant Lymphoid .Proliferations Associated with HIV
Infection
Daniel M. Knowles, M.D.

Special Guest Lecture
Chromosomal Translocations:  Dangerous Liaisons
Janet D. Rowley, M.D.
 
Wednesday
Principles of Lymphoma Classification
Nancy L. Harris, M.D.

Low Grade B-Cell Lymphomas
Nancy L. Harris, M.D.
                                                       
Intermediate and High-Grade  B-Cell Lymphomas, and  Lymphoblastic
Lymphoma
Bharat N. Nathwani, M.D.
 
Post-Thymic T-Cell Lymphomas
Elaine S. Jaffe, M.D.
                                                       
Hodgkin's Disease, Nodular  Lymphocyte Predominance
 Sibrand Poppema, M.D.
                                                        
Other Types of Hodgkin's Disease and  Relationship with Non-Hodgkin's
Lymphomas
Sibrand Poppema, M.D.
 
Pathology of the Spleen
Jerome S. Burke, M.D.

Lymphoproliferative Disorders of the Skin
Hideko Kamino, M.D.

                                                      
Bone Marrow Lymphomas and Related Disorders
Stefano Rosati, M.D.
                                                     
Thursday                                                      
Recent Advances in the Management of   Adult Non-Hodgkin's Lymphomas
Clara D. Bloomfield, M.D.
 
Recent Advances in the Management of  Adult Leukemias
 Clara D. Bloomfield, M.D.
 
Microscopic Anatomy of the Bone Marrow and Hematopoietic Cell
Regulation
Kathy Foucar, M.D.

Reactive Leukocyte Disorders
Kathy Foucar, M.D.

Approach to the Diagnosis of   Acute Leukemias
Robert W. McKenna, M.D.

Acute  Lymphoblastic Leukemias
Kathy Foucar, M.D.

Acute Myeloid Leukemias
Robert W. McKenna, M.D.
                                                   
Myelodysplastic Syndromes 
Stefano Rosati, M.D.

Chronic Myeloproliferative Diseases
James W. Vardiman, M.D.

Friday
B-Cell Chronic Lymphoproliferative  Disorders
Kathy Foucar, M.D.

T Cell Chronic Lymphoproliferative  Disorders
 Robert W. McKenna, M.D.
 
 Plasma Cell Dyscrasias
 Robert W. McKenna, M.D.
  
Interpretation of Post-Therapy Bone Marrows
James W. Vardiman, M.D.
 
Proliferative Disorders of Histiocytes and  Langerhans' Cells
Glauco Frizzera, M.D.
 
 
                                                          
                                                   


 

GENERAL INFORMATION
Location

NYU Medical Center
Alumni Hall
Farkas Auditorium
550 First Avenue, New York, NY

The NYU Medical Center is located in a 
predominately residential area on the
east side of Manhattan.

Accreditation
The NYU Post-Graduate Medical  School is accredited by the
Accreditation Council for Continuing  Medical Education to sponsor
 continuing medical education for physicians. The NYU Post-Graduate
Medical
School designates this continuing  medical education activity for 26.5
credit hours in Category 1 of the Physician's Recognition Award of the
American Medical Association.  The NYU Post-Graduate Medical
School adheres to ACCME Standards regarding industry support of 
continuing medical education. Disclosure of faculty and commercial
relationships,
if any, will be made known at the symposium.

Enrollment
Enrollment is limited. Applications will be accepted in the order of
their
registration form. In the unusual circumstance that this course is
cancelled,
two weeks notice will be given and tuition will be refunded in full.

Information
Registration Office
NYU Post-Graduate Medical School
Murray Hill Station
PO. Box 1855, New York, NY 10016
(212) 263-5295
Please note: We cannot accept phone or fax registration.
Parking
Parking is available at the Kips Bay Parking Garage directly across
from the
Medical Center at 32nd Street (575 First Avenue). There is additional
parking
on 29th Street between First and Second Avenues.

Hotel and Travel Accommodations
Registrants are responsible for their own hotel and travel
arrangements.
A list of nearby hotels offering special rates to course registrants
will be sent
with confirmation of enrollment. For airline reservations, you may
contact
the medical center travel department at (212) 263-8999, or (800)
801-6208 and
indicate you are a registrant in this course.
 


COURSE REGISTRATION

.
                                                     
 2nd Annual Hematopathology Workshop, #452  May 7-10, 1996
                                                    
Social Security # (Must be supplied for confirmation of
reg~stration-for internal records only)

                                                  
Name
                                                 
Address
                                                       

Day Phone                                                          
Fax

Specialty
Degree


Full Tuition: $600
Reduced Tuition: $450
Reduced tuition applies to NYU School of  Medicine alumni, former
residents & fellows;
physicians in training (with certification from  Chief of Service);
technologists; M.D.'s
employed by the Department of Veterans  Affairs Medical Center;
full-time active military personnel; Ph.D.'s; and other health care
professionals.

Cash and credit cards not accepted. Foreign registrants must send an
International Postal Money Order.
Make Check Payable to: 
NYU Post Graduate Medical School

Send to: Registration Department
NYU Postgraduate Medical School
Murray Hill Station
P.O. Box 1855
New York, NY 10016
Refund Policy: $60 will be retained to cover administrative costs. In
order to
receive a refund, written notice must be postmarked two weeks prior to
the course opening.
 The NYU Post-Graduate Medical School reserves the right to cancel a
course.


If you have questions about this course, you can e-mail to Dr.
Inghirami. (inghig01@mcr.cro.med.nyu.edu)



From owner-diagnostics@net.bio.net Mon Mar 18 22:00:00 1996
Path: biosci!IX.NETCOM.COM!temprise
From: temprise@IX.NETCOM.COM
Newsgroups: bionet.diagnostics
Subject: Help
Date: 19 Mar 1996 13:47:46 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 19
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199603192143.NAA11150@ix5.ix.netcom.com>
NNTP-Posting-Host: net.bio.net

To anyone who can help.

My one-year-old grandaughter is very ill. Her white cell blood count is 
zero. 
Although several tests have been made, there is no definitive diagnose. 
One hematologist believes it to be a rare blood disease, possibly, 
"Cyclic 
Neutropenia" but that hasn't been confirmed.

We are located in San Francisco. Can you give me the name of the 
leading 
authority on hematology or other appropriate specialty here or anywhere 
in 
the world? Cost is not important.

Gratefully,

Kenneth Sale
E-Mail: temprise@ix.netcom.com

From owner-diagnostics@net.bio.net Wed Mar 20 22:00:00 1996
Path: biosci!PO.IIJNET.OR.JP!srldx
From: srldx@PO.IIJNET.OR.JP (Hajime Sugisaki)
Newsgroups: bionet.diagnostics
Subject: SRL, Inc., new www site
Date: 21 Mar 1996 01:29:59 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 14
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199603210927.SAA03385@sh0.po.iijnet.or.jp>
NNTP-Posting-Host: net.bio.net

Dear netters, We (SRL, Inc.) are pleased to announce opening of a new www
site (http://www.iijnet.or.jp/SRL-CO/). You will be able to know activities
of this company as a leading commercial reference laboratory in Japan.
Thank you very much for your attention.
H. Sugisaki
Section Chief
Intellectual Property and Licensing Dept.
Reagent Production Dept. (concurrent)
SRL, Inc.
Tel: (Japan)-426-48-4017
Fax: (Japan)-426-48-4087
E-Mail: srldx@po.iijnet.or.jp
http://www.iijnet.or.jp/SRL-CO/


From owner-diagnostics@net.bio.net Wed Mar 20 22:00:00 1996
Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!pasteur.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!newsfeed.internetmci.com!in2.uu.net!vol.net!news.glink.net.hk!hpg30a.csc.cuhk.hk!news.cuhk.edu.hk!newsfeeder.ust.hk!nntp.hk.super.net!news.iij.ad.jp!news2.iij.ad.jp!usenet
From: srldx@po.iijnet.or.jp (srldx)
Newsgroups: bionet.diagnostics
Subject: SRL, Inc., new www site
Date: 21 Mar 1996 09:19:48 GMT
Organization: SRL, Inc.
Lines: 13
Sender: srldx@sh0.po.iijnet.or.jp
Message-ID: <4ir6vk$t50@news2.iij.ad.jp>
NNTP-Posting-Host: ppp8019.po.iijnet.or.jp
X-Posted-From: InterNews 1.0.7@ppp8019.po.iijnet.or.jp
X-Authenticated: srldx on POP host sh0.po.iijnet.or.jp

Dear netters, We (SRL, Inc.) are pleased to announce opening of a new
www site (http://www.iijnet.or.jp/SRL-CO/). You will be able to know
activities of this company as a leading commercial reference laboratory
in Japan. Thank you very much for your attention.

H. Sugisaki
Dept. of Intellectual Property and Licensing
Dept. of Reagent Production (concurrent)
SRL, Inc.
Tel: (Japan)-426-48-4017
Fax: (Japan)-426-48-4087
E-Mail: srldx@po.iijnet.or.jp
http://www.iijnet.or.jp/SRL-CO/

From owner-diagnostics@net.bio.net Wed Mar 20 22:00:00 1996
Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!in1.uu.net!bull.hkstar.net!b1!alo
From: alo@b1.hkstar.com (Lo Wing Ip Anthony)
Newsgroups: bionet.diagnostics
Subject: Re: Microsatellite probes
Date: 21 Mar 1996 16:26:03 GMT
Organization: Princess Margaret Hospital
Lines: 13
Distribution: world
Message-ID: <4irvur$p2r@bull.hkstar.net>
References: <9603071439.aa22043@jura.sasa.gov.uk>
NNTP-Posting-Host: b1.hkstar.com
X-Newsreader: TIN [version 1.2 PL2]

Kevin O'Donnell (odonnell@sasa.gov.uk) wrote:
: Can anyone suggest a supplier of microsatellite probes, ideally 
: biotin labelled?

How about Oncor?


Dr. Anthony W.I. Lo (MB ChB, B Med Sc)     Tel:   (852)-2990-1870
Medical Officer			 	   Fax:   (852)-2370-0969
Chemical Pathology, Dept. of Pathology	   Pager:  7205-4096
Princess Margaret Hospital		   e-mail: anthony-lo@cuhk.hk
Lai King Hill, Kowloon. Hong Kong.	   http://www.hkstar.com/~alo


From owner-diagnostics@net.bio.net Thu Mar 21 22:00:00 1996
Path: biosci!rutgers!uwm.edu!newsfeed.internetmci.com!in1.uu.net!sleepy.inch.com!usenet
From: metin@inch.com
Newsgroups: bionet.diagnostics
Subject: Hematopathology Workshop at NYU
Date: Fri, 22 Mar 1996 15:39:29 GMT
Organization: The Internet Channel
Lines: 348
Message-ID: <4iuaio$cna@sleepy.inch.com>
NNTP-Posting-Host: port123.dialup.inch.com
X-Newsreader: Forte Free Agent 1.0.82

2ND ANNUAL HEMATOPATHOLOGY WORKSHOP AT  NEW YORK UNIVERSITY  (May
7-10)

COURSE DESCRIPTION
This concentrated 3 1/2 day course is designed to provide a
thorough and in-depth review of hematologic and lymphoid
pathology by the leading experts in this field. Both histopatho-
logic criteria and differential diagnosis and data provided by
immunopathology, molecular biology, and cytogenetics will
be discussed. A set of Kodachrome slides will be provided,
illustrating the pathology of post-thymic T-cell lymphomas.

EDUCATIONAL OBJECTIVES
This course is intended for practicing pathologists, residents
and fellows in pathology to update them on recent develop-
ments and the latest diagnostic techniques in current problem
areas.

EXPECTED OUTCOME
It is expected that the participants will acquire a wider knowledge of
 hemato-lymphoid disorders-especially neoplastic, as
well as a definite sense for the morphologic criteria and the
additional specialized studies required for their diagnosis.
                                  
FACULTY

Course Director
Glauco Frizera, M.D.
Professor of Pathology and Director  of Hematopathology
NYU School of Medicine
New York, New York

Co-Directors
Robert W. McKenna, M.D.
Professor and Vice-Chairman
Department of Pathology
Southwestern Medical School
Dallas, Texas

Giorgio Inghirami, M.D.
Associate Professor of Pathology
and Director of Molecular Pathology
NYU School of Medicine
New York, New York

Special Guest Lecturer
Janet D. Rowley, M.D.
Blum-Riese Professor
Departments of Medicine and Molecular Cytogenetics and Cell Biology
University of Chicago School of Medicine
Chicago, Illinois

Guest Faculty
Clara D. Bloomfield, M.D.
Professor of Medicine
Chief, Division of Oncology
State University of New York at Buffalo
Head, Department of Medicine
Roswell Park Cancer Institute
Buffalo, New York

Jerome S. Burke, M.D.
Clinical Professor of Pathology
University of California School of Medicine
San Francisco California
Director of Surgical Pathology
Alta  Bates Medical Center
Berkeley, California

Kathy Foucar, M.D.
Professor of Pathology and Director of the Pathology Service
University of New Mexico Hospital
Albuquerque, New Mexico
 

Nancy L. Harris, M.D.
Associate Professor of Pathology and Director of Anatomic Pathology
Massachusetts General Hospital
Harvard Medical School
Boston, Massachusetts

Elaine s. Jaffe, M.D.
Chief, Hematopathology Section
Laboratory of Pathology
National Cancer Institute
Bethesda, Maryland

Daniel M. Knowles, M.D.,
Professor and Chairman of Pathology
The New York Hospital -Cornell Medical Center
New York, New York

Bharat N. Nathwani, M.D.
Professor of Pathology and Chief of Hematopathology
USC School of Medicine
Los Angeles, California

Sibrand Poppema, M.D.
Professor and Chairman of Pathology
University of Groningen and Academic Hospital
Groningen, The Netherlands

James W. Vardiman, M.D.
Professor of Pathology
Director of Hematology/Hematopathology
University of Chicago Hospital
Chicago, Illinois


NYU Faculty
Hideko Kamino, M.D.
Director of Dermatopathology
NYU Medical Center
New York, New York

Stefano Rosati, M.D.
Assistant Professor
Department of Pathology
NYU School of Medicine
New York, New York

Program Schedule
Tuesday
Normal Lymphoid Tissues:  Review
Nancy L. Harris, M.D.
 
Reactive Lymphadenopathies
Glauco Frizzera, M.D.
 
Atypical Lymphoproliferative Disorders
Glauco Frizzera, M.D.

Immunophenotypic Characterization of  Hematopoietic Malignancies
Giorgio Inghirami, M.D.
 
  
Molecular Genetic Basis of Lymphoid  Neoplasms
Giorgio Inghirami, M.D.

Post-Transplantation Lymphoproliferative  Disorders: Clinical and
Biologic Aspects I
Daniel M. Knowles, M.D.
 
Benign and Malignant Lymphoid .Proliferations Associated with HIV
Infection
Daniel M. Knowles, M.D.

Special Guest Lecture
Chromosomal Translocations:  Dangerous Liaisons
Janet D. Rowley, M.D.
 
Wednesday
Principles of Lymphoma Classification
Nancy L. Harris, M.D.

Low Grade B-Cell Lymphomas
Nancy L. Harris, M.D.
                                                       
Intermediate and High-Grade  B-Cell Lymphomas, and  Lymphoblastic
Lymphoma
Bharat N. Nathwani, M.D.
 
Post-Thymic T-Cell Lymphomas
Elaine S. Jaffe, M.D.
                                                       
Hodgkin's Disease, Nodular  Lymphocyte Predominance
 Sibrand Poppema, M.D.
                                                        
Other Types of Hodgkin's Disease and  Relationship with Non-Hodgkin's
Lymphomas
Sibrand Poppema, M.D.
 
Pathology of the Spleen
Jerome S. Burke, M.D.

Lymphoproliferative Disorders of the Skin
Hideko Kamino, M.D.

                                                      
Bone Marrow Lymphomas and Related Disorders
Stefano Rosati, M.D.
                                                     
Thursday                                                      
Recent Advances in the Management of   Adult Non-Hodgkin's Lymphomas
Clara D. Bloomfield, M.D.
 
Recent Advances in the Management of  Adult Leukemias
 Clara D. Bloomfield, M.D.
 
Microscopic Anatomy of the Bone Marrow and Hematopoietic Cell
Regulation
Kathy Foucar, M.D.

Reactive Leukocyte Disorders
Kathy Foucar, M.D.

Approach to the Diagnosis of   Acute Leukemias
Robert W. McKenna, M.D.

Acute  Lymphoblastic Leukemias
Kathy Foucar, M.D.

Acute Myeloid Leukemias
Robert W. McKenna, M.D.
                                                   
Myelodysplastic Syndromes 
Stefano Rosati, M.D.

Chronic Myeloproliferative Diseases
James W. Vardiman, M.D.

Friday
B-Cell Chronic Lymphoproliferative  Disorders
Kathy Foucar, M.D.

T Cell Chronic Lymphoproliferative  Disorders
 Robert W. McKenna, M.D.
 
 Plasma Cell Dyscrasias
 Robert W. McKenna, M.D.
  
Interpretation of Post-Therapy Bone Marrows
James W. Vardiman, M.D.
 
Proliferative Disorders of Histiocytes and  Langerhans' Cells
Glauco Frizzera, M.D.
 
 
                                                          
                                                   


 

GENERAL INFORMATION
Location

NYU Medical Center
Alumni Hall
Farkas Auditorium
550 First Avenue, New York, NY

The NYU Medical Center is located in a 
predominately residential area on the
east side of Manhattan.

Accreditation
The NYU Post-Graduate Medical  School is accredited by the
Accreditation Council for Continuing  Medical Education to sponsor
 continuing medical education for physicians. The NYU Post-Graduate
Medical
School designates this continuing  medical education activity for 26.5
credit hours in Category 1 of the Physician's Recognition Award of the
American Medical Association.  The NYU Post-Graduate Medical
School adheres to ACCME Standards regarding industry support of 
continuing medical education. Disclosure of faculty and commercial
relationships,
if any, will be made known at the symposium.

Enrollment
Enrollment is limited. Applications will be accepted in the order of
their
registration form. In the unusual circumstance that this course is
cancelled,
two weeks notice will be given and tuition will be refunded in full.

Information
Registration Office
NYU Post-Graduate Medical School
Murray Hill Station
PO. Box 1855, New York, NY 10016
(212) 263-5295
Please note: We cannot accept phone or fax registration.
Parking
Parking is available at the Kips Bay Parking Garage directly across
from the
Medical Center at 32nd Street (575 First Avenue). There is additional
parking
on 29th Street between First and Second Avenues.

Hotel and Travel Accommodations
Registrants are responsible for their own hotel and travel
arrangements.
A list of nearby hotels offering special rates to course registrants
will be sent
with confirmation of enrollment. For airline reservations, you may
contact
the medical center travel department at (212) 263-8999, or (800)
801-6208 and
indicate you are a registrant in this course.
 


COURSE REGISTRATION

.
                                                     
 2nd Annual Hematopathology Workshop, #452  May 7-10, 1996
                                                    
Social Security # (Must be supplied for confirmation of
reg~stration-for internal records only)

                                                  
Name
                                                 
Address
                                                       

Day Phone                                                          
Fax

Specialty
Degree


Full Tuition: $600
Reduced Tuition: $450
Reduced tuition applies to NYU School of  Medicine alumni, former
residents & fellows;
physicians in training (with certification from  Chief of Service);
technologists; M.D.'s
employed by the Department of Veterans  Affairs Medical Center;
full-time active military personnel; Ph.D.'s; and other health care
professionals.

Cash and credit cards not accepted. Foreign registrants must send an
International Postal Money Order.
Make Check Payable to: 
NYU Post Graduate Medical School

Send to: Registration Department
NYU Postgraduate Medical School
Murray Hill Station
P.O. Box 1855
New York, NY 10016
Refund Policy: $60 will be retained to cover administrative costs. In
order to
receive a refund, written notice must be postmarked two weeks prior to
the course opening.
 The NYU Post-Graduate Medical School reserves the right to cancel a
course.


If you have questions about this course, you can e-mail to
 Dr.Inghirami. (inghig01@mcrcr0.med.nyu.edu)




From owner-diagnostics@net.bio.net Thu Mar 21 22:00:00 1996
Path: biosci!daresbury!lyra.csx.cam.ac.uk!sunsite.doc.ic.ac.uk!dundee.ac.uk!pub2.medschool.dundee.ac.uk!kappleyard
From: kappleyard@ninewells.dundee.ac.uk (Keith Appleyard)
Newsgroups: bionet.diagnostics
Subject: Re: Saliva diagnostic kit
Date: Fri, 22 Mar 1996 13:59:24 GMT
Organization: University of Dundee
Lines: 15
Distribution: world
Message-ID: <kappleyard.7.3152B23C@ninewells.dundee.ac.uk>
References: <199603140140.KAA03995@sh0.po.iijnet.or.jp>
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In article <199603140140.KAA03995@sh0.po.iijnet.or.jp> srldx@PO.IIJNET.OR.JP (Hajime Sugisaki) writes:
>From: srldx@PO.IIJNET.OR.JP (Hajime Sugisaki)
>Subject: Saliva diagnostic kit
>Date: 13 Mar 1996 17:43:16 -0800

>Dear netters, I really appreciate if somebody inform me of
>diagnostic/pharmaceutical makers supplying  a diagnostic kit to detect
>antibody (viral and bacterial antibodies) in patient's saliva. Our
>laboratory received the increasing demand from dental offices. Thank you in
>advance.

Keith Appleyard, Senior Scientific/Dept.Safety Officer, 
Virology, Ninewells Hospital and Medical School, Dundee DD1 9SY,U.K
e-mail k.appleyard@dundee.ac.uk
Tel:U.K 01382 632559 Fax:01382 641907

From owner-diagnostics@net.bio.net Fri Mar 22 22:00:00 1996
Path: biosci!mserv1.dl.ac.uk!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!oleane!tank.news.pipex.net!pipex!swrinde!howland.reston.ans.net!psinntp!psinntp!psinntp!psinntp!cmcl2!mcrcr6!oddouc01
Newsgroups: bionet.diagnostics
Subject: Amino acid analyzer for sale
Message-ID: <1996Mar22.222404.8253@mcrcr6>
From: oddouc01@mcrcr6.med.nyu.edu. (oddouc01)
Date: 22 Mar 96 22:24:04 EDT
Nntp-Posting-Host: mcti4mac5.med.nyu.edu
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Equipment for sale at NYU Medical Center

Waters 712 WISP HPLC
Beckman amino acid analyzer, model 7300
ESA HPLC pump 420
Perkin Elmer Auto System Gas Chromatograph

Contact Harry Ostrer
ostreh01@mcrcr6.med.nyu.edu
212 263-5746

From owner-diagnostics@net.bio.net Sat Mar 23 22:00:00 1996
Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!uwm.edu!reuter.cse.ogi.edu!dialin-5.admin.ogi.edu!user
From: sathyam@eeap.ogi.edu (ujwal sathyam)
Newsgroups: bionet.diagnostics
Subject: Resume - Biomedical Engr/Scientist
Date: Sun, 24 Mar 1996 10:37:30 -0800
Organization: oregon medical laser center
Lines: 130
Message-ID: <sathyam-2403961037300001@dialin-5.admin.ogi.edu>
NNTP-Posting-Host: dialin-5.admin.ogi.edu


UJWAL SATHYAM
6320 SW Lombard Street, Apartment 34
Beaverton, OR 97008
Home: (503) 644-3201
Work: (503) 291-2109 or (503) 291-4093
Fax: (503) 291-2422
e-mail: sathyam@eeap.ogi.edu
        ujwal_sathyam@phsor.org

OBJECTIVE:
A challenging career in research and development of non-invasive
techniques for medical diagnosis and therapy



SUMMARY:
Three years of experience in experimental and theoretical
research of laser applications in medicine. Strong background in
electrical engineering, physics, mathematics, and computer
programming. Medical background includes human anatomy and
physiology, and participation in animal trials.


EXPERIENCE:
1992 - present
Research Assistant,
Oregon Medical Laser Center



SKILLS:

Biomedical Engineering:
1. Practical experience with a variety of medical lasers from UV - IR.

2. Design of bench-top experiments to simulate invivo situations.

3. Remote temperature sensing using infrared radiometry.

4. Basic investigations of laser ablation of tissue.

5. Theoretical modeling of light transport in tissue.

6. Spectrophotometric measurement of chromophore levels.

7. Measurement of optical properties of tissue.

8. Light delivery with optical fibers and catheters.

9. Good wet lab skills.

10. Participation in animal trials.

11. Graduate level courses in human anatomy and physiology.


Electrical Engineering:
1. Signal processing techniques in frequency and time domains.

2. Design and analysis of analog and digital electronic circuits.

3. Extensive lab skills with optical and electronic equipment.

4. Design and alignment of optical systems.

5. Modeling of physical processes during laser-tissue interaction.

6. Modeling of stochastic processes, Monte Carlo methods.

7. Image acquisition of high-speed phenomena.

8. Familiar with LABVIEW software.


Computer Skills:
 1. Programming languages: Pascal, C, Fortran.

 2. Environments: Mac-OS, Unix, Windows, DOS.


EDUCATION:
Presently in Ph.D. program in Electrical Engineering.
Oregon Graduate Institute of Science & Technology, Portland, OR.
Expected Graduation: July 1996.

M.S., Electrical Engineering.
Oregon Graduate Institute, July 1994.

Bachelor of Engineering, Electronics & Communications.
Mangalore University, India, June 1992.


RESEARCH:
Laser thrombolysis: Jan.93 - present
Conducted basic research into the mechanism of laser-assisted
arterial recanalization. Optimized laser parameters and
identified principal ablation mechanism. Assisted in animal
trials. Procedure is in clinical trials phase.

Non-invasive detection of structures in human skin: July 95 - Dec.95
Investigated a technique to non-invasively detect structures
embedded in inhomogeneous tissue based on measurement of changes
in surface temperature.

Modeling of light transport in tissue: July 95 - present
Evaluating the applicability of the diffusion approximation to
predict optical penetration depth in tissue.

Speech recognition: Aug.91 - May 92, Mangalore University, India
Implemented a voice command recognition system to recognize
spoken passwords based on end-point location and minimum
prediction residual principle.

Modeling Experience:
Implemented a Monte Carlo algorithm to describe light transport
in turbid tissue (C). Wrote code to determine optical properties
of tissue from surface temperature measurements (PASCAL).
Implemented an algorithm to predict internal temperature
distribution following laser irradiation (PASCAL).

OTHER SKILLS:
1. Experienced at authoring and reviewing technical papers and grants.

2. Speak, read, and write German.

AFFILIATIONS:
Optical Society of America

REFERENCES AND PUBLICATIONS PROVIDED ON REQUEST

From owner-diagnostics@net.bio.net Sat Mar 23 22:00:00 1996
Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!sgigate.sgi.com!news1.best.com!news.aimnet.com!alpha.sky.net!news	
From: Technogoddess <gsprague@sky.net>
Newsgroups: bionet.diagnostics
Subject: Re: Saliva
Date: 24 Mar 1996 03:15:25 GMT
Organization: Laboratory Technology Resources
Lines: 32
Message-ID: <4j2eod$cbd@alpha.sky.net>
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On 4 Mar 1996 HartA@agresearch.cri.nz wrote:
>
> I want to collect human saliva samples. I vaguely recall that it is possible
> to get absorbent pads that can be sucked/ chewed and then removed from the
> subjects mouth. Does anyone know of a source of these? Would the cylindrical
> pads that dentists use to keep their patient's teeth dry be suitable?
>
> Thanks,
>                 Alan Hart
>

You can also find collection kits from Sarstedt and Epitope. I've used both along with the Saliva 
Diagnostics System for various applications. They each have their pros and cons and it could 
depend upon your specific use as to the best one to utilize. I often use the following sources when 
searching for products.

http://www.biosupplynet.com/bsn/

or

http://www.data-transport.com/

Good Luck!

Gwen

<*><*><*><*><*><*><*><*><*><*><*><*>
        >>> Freelance Technogoddess <<<
  "I am NOT unemployed, I'm a consultant."
<*><*><*><*><*><*><*><*><*><*><*><*>



From owner-diagnostics@net.bio.net Sat Mar 23 22:00:00 1996
Path: biosci!SHANI.NET!ffish
From: ffish@SHANI.NET (Falk Fish)
Newsgroups: bionet.diagnostics
Subject: Re: Saliva
Date: 23 Mar 1996 17:11:05 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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NNTP-Posting-Host: net.bio.net

On 4 Mar 1996 HartA@agresearch.cri.nz wrote:
> 
> I want to collect human saliva samples. I vaguely recall that it is possible 
> to get absorbent pads that can be sucked/ chewed and then removed from the 
> subjects mouth. Does anyone know of a source of these? Would the cylindrical 
> pads that dentists use to keep their patient's teeth dry be suitable?
> 
> Thanks,
>                 Alan Hart
> 

Sorry for the late response.  I spend too much time out of the country.

Saliva Diagnostic Systems (SDS), 11719 NE 95th Street, Vancouver, WA 
98682, USA (Not Canada!!!), Ph: 1-800-4-SALIVA, 1-800-472-5482, 
1-206-696-4800, Fax: 1-206-254-742, make saliva collection kits, in a form 
you have described.  In the process, the saliva is diluted 1:1 in a 
storage liquid, which confers long term stability to the antibodies.

You can, of course, use dentist pads.  Let your patients chew on a pad 
for a while.  Extract the saliva by centrifugation or by pressing inside 
a syringe.  Raw saliva is, however, not stable and full of bugs. 
You will have to stabilize it, if 
you want to keep it for a while.


Falk Fish, Tel Aviv, Israel.



From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!scsing.switch.ch!news.rccn.net!morgana.mat.uc.pt!histocentro
From: psantos@gemini.ci.uc.pt (Paulo Santos)
Newsgroups: bionet.diagnostics
Subject: PCR in situ HPV
Date: 25 Mar 1996 12:00:10 GMT
Organization: LUSOTRANSPLANTE
Lines: 14
Message-ID: <4j61sa$7ek@morgana.mat.uc.pt>
NNTP-Posting-Host: histocentro.uc.pt
X-Newsreader: News Xpress Version 1.0 Beta #3

I'm looking for a group working on PCR in situ for HPV, in order to stablish 
contacts for help in starting doing this technique.

A quick answer will be highly apreciated.

Thank you in advance,


Paulo Santos

LUSOTRANSPLANTE                                Fax      +351-39-33674
Centro de Histocompatibilidade do Centro       Telefone +351-39-33693
Apartado 3004                                           +351-39-20527
P-3049 COIMBRA Codex            PORTUGAL

From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!metro!metro!OzEmail!usenet
From: Gary Lum <glum@ozemail.com.au>
Newsgroups: bionet.diagnostics
Subject: MGIT TB by BD
Date: Mon, 25 Mar 1996 21:02:40 +0930
Organization: OzEmail Pty Ltd - Australia
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Does anyone have anything favourable or unfavourable to say about BD's MGIT system for 
Mycobacteriology.
Please E-mail me asap.
-- 
Hope you're having a good day

From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!swidir.switch.ch!scsing.switch.ch!news.rccn.net!morgana.mat.uc.pt!histocentro
From: psantos@gemini.ci.uc.pt (Paulo Santos)
Newsgroups: bionet.diagnostics
Subject: PCR Lyme Disease (DNA extraction protocol from serum)
Date: 25 Mar 1996 11:46:12 GMT
Organization: LUSOTRANSPLANTE
Lines: 16
Message-ID: <4j6124$7ek@morgana.mat.uc.pt>
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X-Newsreader: News Xpress Version 1.0 Beta #3


Does anyone use/know a good protocol for extraction of DNA from serum for PCR 
in order to amplify Borrelia (Lyme disease)?

A quick answer will be highly apreciated.

Thank you in advance.



Paulo Santos

LUSOTRANSPLANTE                                Fax      +351-39-33674
Centro de Histocompatibilidade do Centro       Telefone +351-39-33693
Apartado 3004                                           +351-39-20527
P-3049 COIMBRA Codex            PORTUGAL

From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!EM.AGR.CA!DEBOERS
From: DEBOERS@EM.AGR.CA (Solke Deboer)
Newsgroups: bionet.diagnostics
Subject: Milk in PCR mix
Date: 25 Mar 1996 11:53:48 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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I understand some of you may be interested in our
experience with using BLOTTO (skim milk) in the
PCR mix to minimize inhibition due to the
presence of plant compounds.  It was published in
Nuc. Acids. Res. 23 (1995), 2567-8.  I'd be
interested to hear how well it works for you, or
doesn't.
    Solke


From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!news.unige.ch!news
From: veuthey@sc2a.unige.ch (dugenou qrn)
Newsgroups: bionet.diagnostics
Subject: scanning gels and analyzing scans ?
Date: 25 Mar 1996 15:08:07 GMT
Organization: University of Geneva
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Hello fellow biologists and relatives, 

I am in the process of evaluating/developing/improving  novel software apps 
to analyze 
data obtained after scanning gels (protein, DNA, RNA). 

The basic idea is to apply various sophisticated statistical tools to 
evaluate relationships 
between electrophoretic patterns (bands for 1D/spots for  2D) obtained with 
various 
samples.

The practical outcome is to provide a user friendly Windows App integrated 
with pre-
existing image analysis software packages that accomplish more standard 
tasks  
(delimiting bands, positions, densitometry, integration,  etc).

I am particularly interested in genetic data (genetic typing, pedigree 
analyses, PCR and 
RFLP experiments).

If you are in the habit or need of scanning/digitizing your gels, and are 
then faced with the 
task of comparing samples, I would very much be interested in analyzing a 
set of your 
data.  The acceptable input matrix formats  are ASCII , Excel or dBase.

What may be in for you is the possibility to become a bona fide beta tester 
and end up 
receiving free software that may be very useful for your work.

Thank you for your help.
For replies and additional information mail to ids@planet.ch:

Vanya Loroch

Info-Discovery Systems 
1126 Vaux SWITZERLAND
tel. +41-21-801-68-46
fax +41-21-803-25-85

e-mail: ids@planet.ch




From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!surfnet.nl!swsbe6.switch.ch!scsing.switch.ch!news.rccn.net!morgana.mat.uc.pt!histocentro
From: psantos@gemini.ci.uc.pt (Paulo Santos)
Newsgroups: bionet.diagnostics
Subject: PCR in situ HPV
Date: 25 Mar 1996 12:03:15 GMT
Organization: LUSOTRANSPLANTE
Lines: 13
Message-ID: <4j6223$7ek@morgana.mat.uc.pt>
NNTP-Posting-Host: histocentro.uc.pt
X-Newsreader: News Xpress Version 1.0 Beta #3

I'm looking for a group working on PCR in situ for HPV, in order to stablish 
contacts for help in starting doing this technique.

A quick answer will be highly apreciated.

Thank you in advance,

Paulo Santos

LUSOTRANSPLANTE                                Fax      +351-39-33674
Centro de Histocompatibilidade do Centro       Telefone +351-39-33693
Apartado 3004                                           +351-39-20527
P-3049 COIMBRA Codex            PORTUGAL

From owner-diagnostics@net.bio.net Sun Mar 24 22:00:00 1996
Path: biosci!UNIXG.UBC.CA!xiangl
From: xiangl@UNIXG.UBC.CA (Xiang Li)
Newsgroups: bionet.diagnostics
Subject: Re: Milk in PCR mix (fwd)
Date: 25 Mar 1996 14:38:09 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
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Distribution: world
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I recently used the BLOTTO in my PCR reaction to amplify various genes from 
soil DNA extracts. It works generally well in the presence of some 
possible poly-phenolics compounds in brown colour.

Xiang

Dr. Xiang Li
West-East Centre for Microbial Diversity
University of British Columbia
BC, Canada
---------- Forwarded message ----------
Date: 25 Mar 1996 11:53:48 -0800
From: Solke Deboer <DEBOERS@em.agr.ca>
To: diagnost@net.bio.net
Subject: Milk in PCR mix

I understand some of you may be interested in our
experience with using BLOTTO (skim milk) in the
PCR mix to minimize inhibition due to the
presence of plant compounds.  It was published in
Nuc. Acids. Res. 23 (1995), 2567-8.  I'd be
interested to hear how well it works for you, or
doesn't.
    Solke




From owner-diagnostics@net.bio.net Mon Mar 25 22:00:00 1996
Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!sgigate.sgi.com!nntp.coast.net!howland.reston.ans.net!nntp.crl.com!newshub.cts.com!usenet
From: b3748@cts.com (Bryan Kiehl)
Newsgroups: bionet.diagnostics
Subject: Re: PCR Lyme Disease (DNA extraction protocol from serum)
Date: Tue, 26 Mar 1996 02:27:31 GMT
Organization: CTS Network Services
Lines: 30
Message-ID: <4j7kg4$6kn@news2.cts.com>
References: <4j6124$7ek@morgana.mat.uc.pt>
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X-Newsreader: Forte Free Agent 1.0.82

The group at Mayo Clinic perform this test. You might try to contact
them. The contact is Dr. David Pershing.

Bryan Kiehl
GenBio
GenBio@msn.com

psantos@gemini.ci.uc.pt (Paulo Santos) wrote:


>Does anyone use/know a good protocol for extraction of DNA from serum for PCR 
>in order to amplify Borrelia (Lyme disease)?

>A quick answer will be highly apreciated.

>Thank you in advance.



>Paulo Santos

>LUSOTRANSPLANTE                                Fax      +351-39-33674
>Centro de Histocompatibilidade do Centro       Telefone +351-39-33693
>Apartado 3004                                           +351-39-20527
>P-3049 COIMBRA Codex            PORTUGAL

Bryan Kiehl
b3748@cts.com
San Diego, CA


From owner-diagnostics@net.bio.net Wed Mar 27 22:00:00 1996
Path: biosci!rutgers!gatech!newsfeed.internetmci.com!in1.uu.net!EU.net!Belgium.EU.net!idefix.CS.kuleuven.ac.be!chaos.kulnet.kuleuven.ac.be!news.belnet.be!news.vub.ac.be!sga-pc7
From: godfroid@sga.ulb.ac.be (Edmond Godfroid)
Newsgroups: bionet.diagnostics
Subject: Re: PCR Lyme Disease (DNA extraction protocol from serum)
Date: Thu, 28 Mar 96 10:55:39 GMT
Organization: Université Libre de Bruxelles
Lines: 76
Message-ID: <4jdr7b$11gk_001@ulb.ac.be>
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In article <4j6124$7ek@morgana.mat.uc.pt>,
   psantos@gemini.ci.uc.pt (Paulo Santos) wrote:
>
>Does anyone use/know a good protocol for extraction of DNA from serum for PCR 
>in order to amplify Borrelia (Lyme disease)?
>
>A quick answer will be highly apreciated.
>
>Thank you in advance.
>
>
>
>Paulo Santos
>
>LUSOTRANSPLANTE                                Fax      +351-39-33674
>Centro de Histocompatibilidade do Centro       Telefone +351-39-33693
>Apartado 3004                                           +351-39-20527
>P-3049 COIMBRA Codex            PORTUGAL

Dear Colleague,

We developed a PCR assay specific to B. burgdorferi sensu lato species. Oligonucleotide
primers based on Borrelia burgdorferi sensu lato ospA gene sequences was designed for use
in the PCR to type all (SL primers) or each (GI to GIII primers) of the B. burgdorferi sensu
lato genospecies involved in Lyme disease. These genospecies-specific primers were routinely
used in PCR assay on biological fluids (including serum) from Lyme patients. We prepared serum
as followed :

1. Add 1.5 ml of refrigerated biological fluid at 4 °C in microcentrifuge Eppendorf tube.

2. Centrifuge at 4°C for 10 min. at 12,000 g to pellet spirochetes.

3. Discard the supernatant by aspiration.

4. Add again 1.5 ml of clinical sample in the Eppendorf tube and repeat steps 2 and 3.

5. Wash the pellet with 1.5 ml of PBS.

6. Vortex and centrifuge for 10 min. at 12,000 g at 4 °C and discard the supernatant by aspiration.

7. Repeat steps 5 and 6 twice.

8. Add 30 µl of steril water, vortex to resuspend the pellet and add a drop of mineral oil.

9. Incubate for 10 min. at 100 °C in a water bath.

10. Store the prepared sample at 4°C until use.

This protocol gave us good results published in JCM vol. 33 (March 1995), p 602-608.

To improve the specificity and the sensitivity of our PCR assay and to simplify detection procedures of DNA
fragments from polymerase chain reaction , we have also developed a solid phase sandwich hybridization
system which is now manufactured by the Biocode company (Tel : 3241 / 52.26.36; Fax : 3241 / 52.51.96; Belgium). 
This format was used for the identification of Borrelia burgdorferi sensu lato, the causal agent of Lyme disease.
The system relied on the use of a specific capture probe covalently linked to polystyrene plates and
a specific polybiotinylated detection probe.  DNA fragments, resulting from polymerase chain reaction,
sandwiched between these two probes, were detected via enzymatic color development.

The new detection format outperformed agarose gel electrophoresis of PCR products in sensitivity and specificity.
Moreover, in view of its rapidity and simplicity, the system proved appropriate for the routine diagnostic analysis
of clinical specimens from Lyme disease patients.

Best regards,


Dr. Edmond GODFROID




Edmond Godfroid                godfroid@sga.ulb.ac.be

Universite Libre de Bruxelles   Secretariat Tel : +32-(0)67-88.94.61
Service de Genetique Appliquee                    +32-(0)2-650.94.61
Rue de l'industrie, 24                      Fax : +32-(0)67-88.94.77
B-1400 Nivelles                           Email : genapp@sga.ulb.ac.be

From owner-diagnostics@net.bio.net Thu Mar 28 22:00:00 1996
Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!decwrl!tribune.usask.ca!admin3.usask.ca!chelack
From: chelack@admin3.usask.ca
Newsgroups: bionet.diagnostics
Subject: Looking fo antibody to Ehrlichia risticci (Potomac Horse Fever)
Date: 29 MAR 96 11:43:22 GMT
Organization: University of Saskatchewan
Lines: 15
Message-ID: <29MAR96.11432211@admin3.usask.ca>
NNTP-Posting-Host: admin3.usask.ca

I am, as the title indicates, searching for an antibody to E. risticci the
causative agent of Potomac Horse Fever.  We are developing a test for the
organism in formalin fixed paraffin embedded sections, so ideally the
antibody would be of rabbit, goat, or mouse origin.  Most people are using
convalescent horse serum in an indirect FA test, this source of primary
antibody is not useful for the detection of organisms in tissue sections.
A thousand thanks to anyone who can help me find a source of antibody to
E. risticci.
                email   Chelack@admin3.usask.ca
                tel.    1-306-966-7241
                fax.    1-306-966-7244

                regards

                BJC (Brian)

From owner-diagnostics@net.bio.net Fri Mar 29 22:00:00 1996
Path: biosci!rutgers!mcrcr6!oddouc01
Newsgroups: bionet.diagnostics
Subject: Equipment for sale
Message-ID: <1996Mar29.191503.8266@mcrcr6>
From: oddouc01@mcrcr6.med.nyu.edu. (oddouc01)
Date: 29 Mar 96 19:15:02 EDT
Nntp-Posting-Host: mcti4mac5.med.nyu.edu
X-Posted-From: InterNews 1.0@mcrcr6.med.nyu.edu.
Lines: 10

Equipment for sale at NYU Medical Center

Waters 712 WISP HPLC
Beckman amino acid analyzer, model 7300
ESA HPLC pump 420
Perkin Elmer Auto System Gas Chromatograph

Contact Harry Ostrer
ostreh01@mcrcr6.med.nyu.edu
212 263-5746

From owner-diagnostics@net.bio.net Fri Mar 29 22:00:00 1996
Path: biosci!biosci!not-for-mail
From: viik@ee.tut.fi (Viik Jari)
Newsgroups: bionet.biophysics,bionet.diagnostics,sci.engr.biomed,sci.physics.electromag
Subject: TUTORIAL COURSES in Biomedical Engineering, June 6-9 1996, Tampere, Finland
Date: 29 Mar 1996 19:56:42 -0800
Organization: Tampere University of Technology
Lines: 169
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Xref: biosci bionet.biophysics:1823 bionet.diagnostics:717 sci.engr.biomed:5768 sci.physics.electromag:9270

TUTORIAL COURSES in Biomedical Engineering, June 6 - 9, 1996, Tampere
University of Technology, Tampere, Finland

Tutorial courses (TC) in the fields of bioelectromagnetism, biosensors, and
biomedical signal and image processing will be arranged at the Tampere
University of Technology, Tampere, Finland in connection to the 10th
Nordic-Baltic Conference on Biomedical Engineering and 1st International
Conference on Bioelectromagnetism. The TC are primarily aimed at graduate
students and young researchers of Biomedical Engineering but may well suit
also related fields like Electrical Engineering, Computer Science, Physics,
Clinical Medicine, Biology and Chemical Technology etc. 

The TC comprise of four separate 2-day-course that are Bioelectromagnetism,
Biomedical Signal Processing I, Biosensors and Biomedical Signal Processing
II. These will be lectured at Tampere University of Technology in lecture
rooms 1 and 2.

NOTE: DEADLINE FOR REDUCED REGISTRATION FEE: April 8, 1996

If paid before April 8, the regular registration fee for each course is 1000
FIM. The reduced registration fee for university students is 650 FIM. After
April 8 the registration fee is 1400 FIM. The registration fee covers the
course material, coffees and lunches during the course. Registration form is
on reverse side. Printable registration form and up-to-date information can
be found also from internet (http://www.ee.tut.fi/~nbc96/ preconf.html).

DETAILED COURSE PROGRAMS:

BIOELECTROMAGNETISM
Course material: Textbook "Malmivuo J, Plonsey R: Bioelectromagnetism,
Oxford University Press, 1995, 482 pages". A complimentary copy of the book
is given for every participant. Lecturers: Prof. Jaakko Malmivuo, Ragnar
Granit Institute, Tampere University of Technology, Prof. Robert Plonsey,
Duke University, NC, USA.
Thursday 06.06.1996
09:00 ->        Registration + coffee
10:00 - 10:15	Opening ceremony of the TUTORIAL COURSES
10:15 - 11:00	Introduction
11:15 - 12:00	Anatomical and physiological basis of bioelectromagnetism
12:00 - 13:00	Lunch
13:00 - 13:45	Volume source and volume conductor
14:00 - 14:45	Source-field models
14:45 - 15:15	Coffee
15:15 - 16:00	Bidomain model of multicellular volume conductors
16:15 - 17:00	Theoretical methods for analyzing volume sources and volume
conductors
Friday 07.06.1996
09:00 - 09:45   Theory of biomagnetic measurements
09:45 - 10:15   Coffee
10:15 - 11:00   Electroencephalography and magnetoencephalography
11:15 - 12:00   Electrocardiography, vectorcardiography, magnetocardiography
12:00 - 13:00   Lunch
13:00 - 13:45   Electric and magnetic stimulation of neural tissue
13:45 - 14:15   Coffee
14:15 - 15:00   Cardiac pacing
15:15 - 16:00   Impedance measurements
16:00 - 16:15   Closing remarks and issue of certificates

BIOMEDICAL SIGNAL PROCESSING I: 
Course material: Edited booklet based on lecturing notes Lecturers: Prof.
Jaakko Astola and Prof. Tapio Saramdki, Digital Signal Processing
Laboratory, Tampere University of Technology, DrTech Pauli Kuosmanen, and
MSc Sakari Alenius, Digital Media Institute, Tampere University of Technology
Thursday 06.06.1996
09:00 ->	Registration + coffee
10:00 - 10:15	Opening ceremony of the TUTORIAL COURSES
10:15 - 12:00	How to filter an continuous-time signal with the aid of a
digital filter
12:00 - 13:00	Lunch
13:00 - 13:45	Basic tools for analysing and synthisizing digital filters
(Saramdki)
14:00 - 14:45	Filter design and finite wordlength effects (Saramdki)
14:45 - 15:15	Coffee
15:15 - 17:00	Fundamentals of median type filtering (Astola)

Friday 07.06.1996
09:00 - 09:45   Image perception (Kuosmanen)
09:45 - 10:15   Coffee
10:15 - 12:00	Median type filters in biomedical signal processing (Astola)
12:00 - 13:00	Lunch
13:00 - 13:45	Image enhancement (Kuosmanen)
13:45 - 14:15	Coffee
14:15 - 15:00	Image restoration (Kuosmanen)
15:15 - 16:00	Image reconstruction - an example case (Alenius)
16:00 - 16:15	Closing remarks and issue of certificates

BIOSENSORS 
Course material: Edited booklet based on lecturing notes. Lecturers: Prof.
Anthony P.F. Turner, Cranfield Biotechnology Centre, UK, Docent Jukka
Lekkala, Ragnar Granit Institute, Docent Janusz Sadowski, Lic.Phil.
Inger.Vikholm, Lic.Tech. Jyrki Kimmel, VTT Chemical Technology, Tampere.

Saturday 08.06.1996
09:00 -> 	Registration
10:00 - 10:45	Introduction to biosensor concept (Lekkala)
10:45 - 11:00	Coffee
11:00 - 11:45	Biosensing (Vikholm)
11:45 - 12:30	Formation of protein layers (Vikholm)
12:30 - 13:30	Lunch
13:30 - 14:15	Electrochemical methods (Albers)
14:30 - 15:15	Optical sensing by evanescent methods (Sadowski)
15:15 - 15:45	Coffee
15:45 - 16:30	Optical fibre sensors (Kimmel)
16:45 - 17:30	Piezoelectric sensors (Lekkala)

Sunday 09.06.1996 
09:00 - 09:15	Introduction (Lekkala)
09:15 - 10:00	Applications of biosensors (Turner)
10:00 - 10:15	 Coffee
10:15 - 11:30	The "top ten" of commercial biosensors (Turner)	
11:30 - 12:30	Lunch
12:30 - 13:15	Demonstration of biosensors (Lekkala, Turner)
13:15 - 13:45	Coffee
13:45 - 14:30	The future of biosensor research (Turner)
14:30 - 14:45	Closing remarks and issue of certificates (Lekkala)

BIOMEDICAL SIGNAL PROCESSING II
Course material: Edited booklet based on lecturing notes. Lecturers: Prof.
Metin Akay, Biomedical Engineering Department, Rutgers University, NJ, USA,
Prof. Patrick Flandrin, ENS Lyon, France, Prof. Andrew Laine, Computer
Information Science Department, University of Florida, FL, USA, Prof.
Klaus-Peter Adlassnig, University of Vienna Medical School, Vienna, Austria

Saturday 08.06.1996
09:00 ->	Registration
10:00 - 10.45	Fuzzy Logic and Knowledge Based System (Adlassnig)
10:45 - 11:00	Coffee
11:00 - 12:30	Time-Frequency and Wavelets (Flandrin)
12:30 - 13:30	Lunch
13:30 - 15:15	Implementation of 2-D Wavelets (Laine)
15:15 - 15:45	Coffee
15:45 - 17:30	Wavelets and Biomedical Signal Processing (Akay)

Sunday 09.06.1996
09:00 - 09:45	Wavelets and Medical Imaging (Laine)
09:45 - 10:00	Coffee
10:00 - 11:30	Fuzzy Logic and knowledge Based Systems in Medicine (Adlassnig)
11:30 - 12:30	Lunch
12:30 - 13:15	Recent Advances in Time-Freq and Wavelets (Flandrin)
13:15 - 13:30	Coffee
13:30 - 14:15	Hybrid Signal Processing (Akay)
14:15 - 14:30	Closing remarks and issue of certificates

---------
GENERAL INFORMATION:

For the participants of tutorial courses we have reserved following hotels
with reduced conference prices. The prices apply providing that you  contact
the hotel directly and use the indication "NBC96" when making your
reservation. Hotel Victoria: Located in the vicinity (100m) of the congress
center (Tampere Talo). Good bus connection to the Tampere University of
Technology (about 25 min). RR: Single room FIM 350, Double room FIM 420.
Tel.+358-31-2425111 Fax. +358-31-2425100.
Hotel Hermica/Hervanta: Located in the vicinity (400m) of the Tampere
University of Technology. RR: Single room FIM 190, Double room FIM 240. Tel.
+358-31-255 0000, Fax. +358-31-255 0009.

More information gives:
===========================================================================
	Rami Lehtinen, Lic.Tech.
	Ragnar Granit Institute, Tampere University of Technology
	P.O.Box 692, FIN-33101 Tampere, FINLAND
	Tel.+358-(9)31-247 4006, Fax.+358-(9)31-247 4013,
	E-mail rami@ee.tut.fi, WWW-homepage http://www.ee.tut.fi/~rami/
===========================================================================

-- 
	Jari Viik	viik@ee.tut.fi		TTKK/RGI


From owner-diagnostics@net.bio.net Fri Mar 29 22:00:00 1996
Path: biosci!agate!ihnp4.ucsd.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news
From: John.Harrison@mailbox.uq.oz.au (John Harrison)
Newsgroups: bionet.diagnostics
Subject: Diagnosing Pelezaeus - Merzbacher Syndrome??
Date: Sat, 30 Mar 1996 20:50:28 GMT
Organization: University of Queensland
Lines: 3
Message-ID: <4jk6nr$p2@hobyah.cc.uq.oz.au>
NNTP-Posting-Host: sujharri.slip.cc.uq.oz.au
X-Newsreader: Forte Free Agent 1.0.82

Anyone out there interested in doing some genetic testing for this
disorder - apart from M. Hodes at Indiana?


From owner-diagnostics@net.bio.net Sat Mar 30 22:00:00 1996
Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!hobyah.cc.uq.oz.au!bunyip.cc.uq.oz.au!news
From: sv155304@student.uq.edu.au
Newsgroups: bionet.diagnostics
Subject: A New Virological and Molecular Biological Homepage!
Date: Sun, 31 Mar 1996 03:49:02 GMT
Organization: University of Queensland
Lines: 31
Message-ID: <4jkvei$epr@hobyah.cc.uq.oz.au>
NNTP-Posting-Host: svimacka.slip.cc.uq.oz.au
X-Newsreader: Forte Free Agent 1.0.82

To all with an interest in the fields of virological and molecular
biological science.  I draw your attention to a homepage which has
just been released to the Internet.

The Sir Albert Sakzewski Virus Research Centre Homepage

http://florey.biosci.uq.edu.au/SASVRC/sasvrc.htm

With the help of the University of Queensland, we at the Centre have
produced a page which we hope will provide educational information and
peer contact between our Centre, and the rest of the scientific and
general community.

The page is slowly being registered with the various searching engines
on the 'Net including "Webcrawler" and "Lycos" and with related pages,
such as the "Garry Homepage".

Please consider stopping by and adding the address to your bookmark
list.  The page will be continually growing and adapting and will
therefore rely on you, the viewer, for critical appraisal.  Comments
about anything from content to background colour will be recieved
eagerly.

Thankyou,

Ian Mackay
Clinical Virology Research Unit
Sir Albert Sakzewski Virus Research Centre
Brisbane
Queensland


From owner-diagnostics@net.bio.net Sat Mar 30 22:00:00 1996
Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!usc!news.cerf.net!news
From: "N. Gat" <oksi@cerfnet.com>
Newsgroups: bionet.biophysics,bionet.diagnostics,sci.engr.biomed,sci.physics.electromag
Subject: Re: TUTORIAL COURSES in Biomedical Engineering, June 6-9 1996, Tampere, Finland
Date: Sat, 30 Mar 1996 23:38:24 -0800
Organization: Opto-Knowledge Systems, Inc.
Lines: 25
Message-ID: <315E3670.3CE1@cerfnet.com>
References: <4jfubr$ajd@ee.tut.fi>
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Mime-Version: 1.0
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To: Viik Jari <viik@ee.tut.fi>
Xref: biosci bionet.biophysics:1824 bionet.diagnostics:720 sci.engr.biomed:5776 sci.physics.electromag:9295

Hi,

Just in case you are unfamiliar with the TechExpo Calendar of science and
technology conferences (covering the engineering and medical/life
sciences)...  You may want to post your conference
announcement at URL:  http://www.techexpo.com/

This site is visited by over a thousand engineers, scientists, medical
researchers, and technical managers each day.   It is fully indexed and
searchable by event name, location, date, sponsoring org., key words,
etc.  Simply fill out the form with the conference information and
submit.

Please READ posting policy.

Complete conference agenda, and electronic registration form can also be
posted for a nominal fee  For example see: 
http://www.techexpo.com/events/isas96/isas96.html).

Regards,

Nahum Gat, Ph.D.
Please ignore my message if you have already been there.

P.S.  The TechExpo Technical Bookfair is now online!

From owner-diagnostics@net.bio.net Sun Mar 31 23:00:00 1996
Path: biosci!rutgers!gatech!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!dial
From: sc002@dial.oleane.com (COGET Stephane)
Newsgroups: bionet.diagnostics
Subject: Thalassemia problem
Date: Mon, 01 Apr 1996 00:32:24 GMT
Organization: Oleane - PIPEX Internatinal
Lines: 52
Message-ID: <4jn8fm$oh8@Helene.OLEANE.NET>
Reply-To: sc002@dial.oleane.com (COGET Stephane)
NNTP-Posting-Host: dyn-20.vin.oleane.com

Young man of 30, who have never had before any sign, began to 
have, after a kind of infuenza, some sort of very light 
swimmings of the head (without any loss of stability).
After several tests (scanner ..) which were negative and a 
test for blood pathology, he came out he had a minor 
Thalassemia.
Questions :
- How comes he had never had before any trouble due to 
Thalassemia until the age of 29.
- Do you think swimmings of the head are because of 
Thalassemia ?
- The only medecine given to him is 'magnesium' and 'acid 
folique'. The result is not very positive because he goes on 
having these light swimmings of the head, which is very 
embarrassing.
- What is the medecine or treatment indicated for that kind of 
disease ?
 - Do you know any center or hospital specialized on that 
disease.

Here after, a part of the blood pathology tests.
COMPLETE BLOOD COUNT
WBC					8.4
RBC			6.08
HGB			11.09
HCT			37.3
MCV			61.0
MCH			19.6
MCHC				32
RDW			15.1
MPV					9
Platelet count				321

HGB  Electrophoresis
Possible increase in Hemoglobin A2.   HGB A2 quantification by 
column chromatography is suggested.
HGB A		95.4
HGB A2		4.60
HB A2 by column	4.60
HB F by column			0.80

DIFFERENTIAL
Poly, Lymph, Mono, Eos, Baso, Ani socytosis, Hypochromia, 
Microcytosis, Leukocyte morphology appears normal.

IRON test in profile A appears at 82 ug/dL
Haptoglobine results at 0.38 g/l

We apologize for our english which is probably not very 
correct and thanks in advance for your help.

Mail address :sc002@dial.oleane.com

From owner-diagnostics@net.bio.net Sun Mar 31 23:00:00 1996
Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!news.sprintlink.net!new-news.sprintlink.net!rob.inetdirect.net!p5-term2.inetdirect.net!user
From: brent@inetdirect.net (Brent)
Newsgroups: bionet.diagnostics
Subject: mega diagnostic equipment
Date: Sun, 31 Mar 1996 23:20:03 -0600
Organization: AXIS 3
Lines: 3
Message-ID: <brent-3103962320030001@p5-term2.inetdirect.net>
NNTP-Posting-Host: p5-term2.inetdirect.net



http://www.camdon.com/medical/

From owner-diagnostics@net.bio.net Sun Mar 31 23:00:00 1996
Path: biosci!aol.com!MHughes77
From: MHughes77@aol.com
Newsgroups: bionet.diagnostics
Subject: Hepatitis C tests
Date: 1 Apr 1996 09:38:33 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <960401123516_459742875@emout10.mail.aol.com>
NNTP-Posting-Host: net.bio.net

Bionet Diagnostics newsgroup members:

I would like to locate a manufacturer of rapid diagnostic test kits for
Hepatitis C.
Any help would be greatly appreciated.

Mark Hughes
MHughes77@aol.com

