From owner-diagnostics@net.bio.net Mon Apr 01 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!news From: Dr Richard Stitson Newsgroups: bionet.diagnostics Subject: Beta Thalassaemia Minor Date: Tue, 02 Apr 1996 10:59:17 +0000 Organization: Genetics Department Lines: 11 Message-ID: <31610885.2A47@mole.bio.cam.ac.uk> NNTP-Posting-Host: png-mac9.gen.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) Minor Thalassaemia (Beta) is the term used to describe heterozygotes for Beta Thalassaemia (ie one normal gene, one abnormal gene). Rather than the severe symptoms of "major" thalassaemia, minors usually cope ok except when stressed eg during pregnancy. Obviously light headedness can be a symptom of anaemia - although symtoms shouldn't normally occur with a Hb of >11. As for test reults, you would expect Hb 9-11, MCV <75, increased HbA2 as you described. Hope this info is satisfactory. Richard Stitson From owner-diagnostics@net.bio.net Mon Apr 01 23:00:00 1996 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!lyra.csx.cam.ac.uk!news From: Dr Richard Stitson Newsgroups: bionet.diagnostics Subject: Re: Help Date: Tue, 02 Apr 1996 13:04:41 +0000 Organization: Genetics Department Lines: 27 Message-ID: <316125E9.2F5@mole.bio.cam.ac.uk> References: <199603192143.NAA11150@ix5.ix.netcom.com> NNTP-Posting-Host: png-mac9.gen.cam.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) To: temprise@IX.NETCOM.COM I can't give you any details of "experts" but some background: Cyclical neutropenia is characterised by episodes of severe neutropenia and the associated susceptibility to severe, possibly life threatening infections. It may occur alone or as part of various rare syndromes - is the child otherwise completely normal? Treatment with steroids as necessary sometimes works. temprise@IX.NETCOM.COM wrote: > > To anyone who can help. > > My one-year-old grandaughter is very ill. Her white cell blood count is > zero. > Although several tests have been made, there is no definitive diagnose. > One hematologist believes it to be a rare blood disease, possibly, > "Cyclic > Neutropenia" but that hasn't been confirmed. > > We are located in San Francisco. Can you give me the name of the > leading > authority on hematology or other appropriate specialty here or anywhere > in > the world? Cost is not important. > > Gratefully, > > Kenneth Sale > E-Mail: temprise@ix.netcom.com From owner-diagnostics@net.bio.net Tue Apr 02 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.diagnostics Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 3 Apr 1996 02:01:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 149 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604031000.CAA13613@net.bio.net> NNTP-Posting-Host: net.bio.net I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We have three sponsors to date with a couple more pending. The process is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we hope to have new and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Statistics are for the four week period from 22 Jan. - 18 Feb. 1996 and usage continues to grow. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5000 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 600 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. ---------------------------------------------------------------------- From owner-diagnostics@net.bio.net Tue Apr 02 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!news.interserv.net!news1.sprynet.com!news From: nahama Newsgroups: bionet.diagnostics Subject: Looking for equipment Date: Wed, 03 Apr 1996 16:20:09 -0800 Organization: Sprynet News Service Lines: 11 Message-ID: <316315B9.7863@sprynet.com> NNTP-Posting-Host: dd05-054.compuserve.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) hello, I am looking for : - 3000 l capacity (2000l working) fermentors : 2 units - 5000 l jacketed media tank : 1 unit Used but recent (less than 3 years). If you have any contact or know people selling such equipment please email me to 104032.3355@compuserve.com thank you. From owner-diagnostics@net.bio.net Thu Apr 04 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!newsfeed.sunet.se!news00.sunet.se!sunic!mn6.swip.net!plug.news.pipex.net!pipex!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!user From: cliff@cright.demon.co.uk (Cliff) Newsgroups: bionet.diagnostics Subject: Churg - Strauss syndrome (help wanted) Date: Fri, 05 Apr 1996 21:29:01 +0100 Organization: ContactRight Ltd Lines: 35 Message-ID: NNTP-Posting-Host: cright.demon.co.uk X-NNTP-Posting-Host: cright.demon.co.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit If this article is not relevant to this newsgroup, please excuse me posting it here. I have tried to narrow the target to newsgrups I think may be focussed on this subject. I tried posting this a week ago but have not seen it propagated through any news feeds since then. I have a friend who has been suffering for the last few months with a breathing problem and the medics cannot establish the cause. Just recently, by a process of eliminaton, they are beginning to suspect a condition known as Churg - Strauss Syndrome. Can anyone offer any pointers to information on this condition. Please feel free to e-mail me directly or post replies here. I am looking for citations in medical and other journals, contacts, labs or scientists working in this area or resources on the internet that may provide some useful data I can pass to my friend's medics. I'm not intimate with the exact details of her medical condition but there may be some connection with the fact ther her son has athsma, the family has a dog and she is a little overweight. Whether any of these are a factor in the condition is open to question. Her symptoms exhibit themselves as severe breathing difficulties and rapid weight loss when an attack happens. I understand, she has been prescribed some steroid treatment and that this may have to be a long term medication. At present, we have very little information to go on so anything that you can dig up is going to be helpful. Best regards Cliff Wootton From owner-diagnostics@net.bio.net Fri Apr 05 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!rain.fr!imaginet.fr!national11.imaginet.fr!user From: philippe@imaginet.fr (philippe) Newsgroups: bionet.diagnostics Subject: looking for osteopathy Date: 6 Apr 1996 21:19:35 GMT Organization: ImagiNET Lines: 4 Message-ID: NNTP-Posting-Host: max44.imaginet.fr X-Newsreader: Value-Added NewsWatcher F2.1d3+ hi, does anyone know where i can find ingfo on the net on ostheopathy (web, gopher, news) thanks to respond to philippe @imaginet.fr From owner-diagnostics@net.bio.net Fri Apr 05 23:00:00 1996 Path: biosci!MAIL.UTEXAS.EDU!gwpeterson From: gwpeterson@MAIL.UTEXAS.EDU Newsgroups: bionet.diagnostics Subject: (none) Date: 6 Apr 1996 12:12:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-diagnostics@net.bio.net Fri Apr 05 23:00:00 1996 Path: biosci!MAIL.UTEXAS.EDU!gwpeterson From: gwpeterson@MAIL.UTEXAS.EDU Newsgroups: bionet.diagnostics Subject: unsubscribe Date: 6 Apr 1996 12:12:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net From owner-diagnostics@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!UICVM.CC.UIC.EDU!U14663 From: U14663@UICVM.CC.UIC.EDU ("Richard A. Murphy ") Newsgroups: bionet.diagnostics Subject: Re: Staphylococci Date: 10 Apr 1996 12:43:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604101943.MAA20574@net.bio.net> NNTP-Posting-Host: net.bio.net Vigfrid Ness writes: > Is there a quick method to determine if a culture of > Staphylococci consists of both catalase positive and negative > organism. If the culture is catalase negative then there is no > Staph. aureus present, but what I want is an equally simple > methode to determine if there is no catalase negative organisms > present. > The way this is written makes me wonder if you are interested in coagulase rather than catalase. Dick Murphy +-------------------------------------------------------------------+ |Richard A. Murphy, PhD Bitnet: U14663@uicvm.bitnet | |University of Illinois at Chicago Internet: U14663@uicvm.uic.edu | |Department of Oral Medicine Fax: 1-312-996-1022 | | and Diagnostic Sciences Phone: 1-312-996-8630 | |801 S. Paulina | |Chicago, IL 60612 | +-------------------------------------------------------------------+ From owner-diagnostics@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!CHEMISTRY.COM!FHeasley From: FHeasley@CHEMISTRY.COM (DrHeasley) Newsgroups: bionet.diagnostics Subject: Re: Staphylococci Date: 10 Apr 1996 11:07:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604101802.OAA50252@osceola.gate.net> NNTP-Posting-Host: net.bio.net At 05:26 PM 4/10/96 +0100, vigfrid.ness@veths.no (Vigfrid Ness) wrote: >Hi! >Is there a quick method to determine if a culture of Staphylococci consists >of both catalase positive and negative organism. If the culture is catalase >negative then there is no Staph. aureus present, but what I want is an >equally simple methode to determine if there is no catalase negative >organisms present. > >Can anyone help me???? > >Vigfrid Ness > > There are catalase negative organisms present in virtually all staphylococcal cultures, because there are spontaneous mutants present in all of them. The issue is whether the number of catalase negative forms is significant. If you're concerned about a significant percentage, then you can make a good streak plate and test the separated colonies as usual with peroxide, observing carefully whether there are colonies that do not exhibit characteristic signs. If you are concerned with extremely low numbers (like 1 in 10^6) then you will need to use a selection process to separate and enrich for them. Frank Heasley, Ph.D. Principal FSG MEDICAL AND SCIENTIFIC EMPLOYMENT HTTP://WWW.CHEMISTRY.COM/JOBS From owner-diagnostics@net.bio.net Tue Apr 09 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: vigfrid.ness@veths.no (Vigfrid Ness) Newsgroups: bionet.diagnostics Subject: Staphylococci Date: 10 Apr 1996 17:26:27 +0100 Lines: 11 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4kgnfj$sc5@mserv1.dl.ac.uk> X-Sender: nmhness@samson.veths.no Original-To: diagnost@dl.ac.uk Hi! Is there a quick method to determine if a culture of Staphylococci consists of both catalase positive and negative organism. If the culture is catalase negative then there is no Staph. aureus present, but what I want is an equally simple methode to determine if there is no catalase negative organisms present. Can anyone help me???? Vigfrid Ness From owner-diagnostics@net.bio.net Wed Apr 10 23:00:00 1996 Path: biosci!CHEMISTRY.COM!FHeasley From: FHeasley@CHEMISTRY.COM (DrHeasley) Newsgroups: bionet.diagnostics Subject: GROUP MANAGER, PhD, Automated Immunodiagnostics Date: 11 Apr 1996 14:59:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604112044.QAA99674@osceola.gate.net> NNTP-Posting-Host: net.bio.net We have been retained by an internationally recognized client located in the northern united states to identify and recruit a Group Manager for their automated immunodiagnostics research and development efforts. Reporting to the Director of Research and Development, The Group Manager is responsible for the management of multiple development projects and the supervision of up to five Project Leaders. Additional responsibilities will include due diligence evaluations and technical support functions in coordination with marketing activities. Requirements include excellent interpersonal and communications skills, and demonstrated ability to lead multiple immunodiagnostic product development teams. A Ph.D. in a related field will be strongly preferred. Comprehensive relocation assistance will be provided. Candidates who meet all of the above qualifications are invited to forward resumes for confidential consideration. All applications will be thoroughly evaluated. Frank Heasley, Ph.D. Principal The Franklin Search Group, Inc. 5632 SW 88 Terrace Cooper City, FL 33328 FAX 954-434-4840 FHeasley@Chemistry.com The Franklin Search Group, Inc. is an executive search organization focused exclusively in the recruitment of management positions where scientific expertise is required. All fees are paid by the clients who retain our services. Our clients are equal opportunity employers. Candidates seeking other positions may inquire using the contact information above, and should also visit our web: http://www.chemistry.com/jobs From owner-diagnostics@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!gatech!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!news.cyberenet.net!news From: kline@cyberenet.net (Mark Kline) Newsgroups: bionet.diagnostics Subject: frequent nosebleeds Date: Fri, 12 Apr 1996 20:52:00 GMT Organization: CyberENET Networks and Systems, 609 753 9840; dialup 609 753 1572 and 800 356 3638 Lines: 10 Message-ID: <4km59u$f62@news.cyberenet.net> NNTP-Posting-Host: kline.ppp.cyberenet.net X-Newsreader: Forte Free Agent 1.0.82 My son, who is almost 10 suffers from frequent nosebleeds, (3x a month) the last time mas during the middle of the night. He is otherwise a very healthy kid. Is there something I need to do, can this be a symptom of something more serious,? Mark Kline kline@cyberenet.net From owner-diagnostics@net.bio.net Thu Apr 11 23:00:00 1996 Path: biosci!MAIL.UTEXAS.EDU!gwpeterson From: gwpeterson@MAIL.UTEXAS.EDU Newsgroups: bionet.diagnostics Subject: (none) Date: 12 Apr 1996 13:07:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe: diagnost@net.bio.net From owner-diagnostics@net.bio.net Fri Apr 12 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!news.interserv.net!news1.sprynet.com!news From: nahama Newsgroups: bionet.diagnostics Subject: Info needed Date: Fri, 12 Apr 1996 18:35:00 -0700 Organization: Sprynet News Service Lines: 15 Message-ID: <316F04C4.480F@sprynet.com> NNTP-Posting-Host: dd05-099.compuserve.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Win16; I) Hello, I am actually trying to make a survey of the number of diagnostics tests (for each disease and species) performed in the different AMERICAN or SOUTH-AMEICAN diagnostic labs (1995). [mostly serological analysis] I would be grateful for any information you could send me about this subject. All data received will be considered confidential. Thank you. For further information or mailing address e-mail me at 104032.3355@compuserve.com Thank you for your time Alexis G NAHAMA, DVM. From owner-diagnostics@net.bio.net Fri Apr 12 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.sprintlink.net!new-news.sprintlink.net!misc.twics.com!news-stock.gsl.net!news-dc.gsl.net!news-lond.gsl.net!speedy.grolier.fr!rain.fr!news From: Laurent BOUSQUET Newsgroups: bionet.diagnostics Subject: information about rapid tests Date: Sat, 13 Apr 1996 15:35:46 -0700 Organization: CSI InterNetNews site Lines: 4 Message-ID: <31702C42.6F33@accescyb.fr> NNTP-Posting-Host: ppp13.accescyb.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b6a (Win16; I) I am looking for a company which provides manufacturing custom rapid test using reagents and components furnished by my laboratory. Emmanuelle From owner-diagnostics@net.bio.net Sat Apr 13 23:00:00 1996 Path: biosci!rutgers!uwm.edu!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: krokos@ix.netcom.com(Christopher A Krokos * USA *) Newsgroups: bionet.diagnostics Subject: Q: GLP's - Good Laboratory Practice Standards ? Date: 13 Apr 1996 20:06:27 GMT Organization: Netcom Lines: 11 Message-ID: <4kp1g3$8eh@reader2.ix.netcom.com> NNTP-Posting-Host: nhv-ct1-07.ix.netcom.com X-NETCOM-Date: Sat Apr 13 1:06:27 PM PDT 1996 Q: GLP’s - Good Laboratory Practice Standards ? I need a general info about GLP’s for my job interview. You may also direct me to the appropriate WebSite. Thank you for any information, Christopher A Krokos krokos@ix.netcom.com From owner-diagnostics@net.bio.net Sun Apr 14 23:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!swrinde!news.uh.edu!usenet From: pillai@elm.egr.uh.edu (Bipin K. Pillai) Newsgroups: bionet.diagnostics Subject: restrictive cardiomyopathy Date: 15 Apr 1996 23:10:48 GMT Organization: University of Houston Lines: 71 Message-ID: <4kul1o$7ei@masala.cc.uh.edu> NNTP-Posting-Host: elm.egr.uh.edu Hi !! Please excuse me if this is not the right place for posting such articles. Any suggestions of where i could post such an article would be appreciated. I am posting this for a friend's friend's friend's brother (I am serious). He is suffering from cardic problem, his heart's elasticity is reduced, which makes it difficult to pump blood. Doctor have given up hope. He is lying in intensive care unit. I was sent the following synopsis: Dr. Ashok H. Punjabi (CARDIOLOGIST) DISCHARGE SUMMARY NAME: MR SUSHIL JAIN AGE: 48 YEARS SEX:MALE Date Of Admission:1.26.96 Date Of Discharge:2.4.96 SUMMARY Mr. Sushil Jain, a 48 year old male, and diagnosed case of restrictive cardiomyopathy has been admitted with c/o giddiness and syncopal attacks - 2 hours prior to admission and vomiting the previous day. There was h/o fever,seizures or oliguria. There was no chest pain. On admission, he was conscious, alert with pulse of 96/min and BP of 70 mm Hg systolic. The JVP was raised and there was marked oedema feet. Systemic examination revealed hepatomegaly and free fluid in the abdomen. The scope showed runs of broad QRS tachycardia. (? ventricular tachycardia, ? SVT with aberrant conduction) which responded to IV bolus Xylocard. 1.26.96 1.27.96 1.28.96 1.29.96 1.31.96 BUN 21 33 S.Creat 1.9 2.6 2.2 0.9 0.8 Na 124 123 122 133 K 7.5 6.6 4.4 3.9 4.8 Cl 95 92 87 96 TCO2 14.4 17.6 20.2 SGO2 798 Bili T 3 D 2.4 In view of hyperkalemia and azoremia nephrology, opinion by Dr. Hemant Metha was taken and he was treated with glucose insulin drip, Kay Exalate powder, Lasix, Sodabicarb, Calcium gluconate and Sporidex. His urine output gradually improved and the azotemia settled down. During the stay, he develpoed constipation which did not respond to Laxatives or simple enema. Dr. Anand Nande (Consultant surgeon) did manual removal of faeces and the patient improved thereafter with neotomic enema. He was discharged on following medication on 2.4.96 : TABLET ANTIDEP 25 mg O---O---1 TABLET SUPRADYN 1 OD TABLET LASIX 40 mg 1--O---O SYRUP CREMAFFIN 2 TSP AT BED TIME. Patient develops similar symptoms on 4.10.96 and admitted to hospital in ICU again on same day. Has anyone handled a similar case/situation. Any assistance (suggestions) regarding this would be deeply appreciated. You can send the mail to BCSSADM0@giasbm01.vsnl.net.in or blips@uh.edu. Thanks. Bipin Pillai From owner-diagnostics@net.bio.net Mon Apr 15 23:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!psinntp!psinntp!psinntp!news.nstn.ca!news From: advresum@fox.nstn.ca (advresum) Newsgroups: bionet.diagnostics Subject: Sore and swollen LEGS...HELP!!! Date: 16 Apr 1996 16:50:57 GMT Organization: NSTN Navigator User Lines: 6 Message-ID: <4l0j5h$2uc@news.nstn.ca> NNTP-Posting-Host: truro-ts-04.nstn.ca Mime-Version: 1.0 X-Newsreader: WinVN 0.93.14 I'm 31 yrs old, 185 lbs, fair physical cond and yet my legs are very sore, swollen from the knees down, and at times sharp pain. Several trips to doctor haven't helped. Catscans, ultrasounds, and bloodtests turned up nothing. My doctor is stumped but I do not wish to have stumps for legs. Any ideas? It's been like this for the past 6 months or so. From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!news1.erols.com!newsfeed.internetmci.com!in2.uu.net!news.ios.com!usenet From: krisprasad Newsgroups: bionet.diagnostics Subject: Re: information about rapid tests Date: 17 Apr 1996 21:15:53 GMT Organization: Internet Online Services Lines: 29 Message-ID: <4l3n29$1v7@news.ios.com> References: <31702C42.6F33@accescyb.fr> NNTP-Posting-Host: ppp-52.ts-9.hck.idt.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) To: emmanuelle,at,Laurent,Bousquet DO you mean if we will manufacture the product to your specifications using your reagents? If yes, we would like to get some more information to see if we can manufacture your product. email:prasad@village.ios.com Vipra, Inc. Tel: 201 489-8450 Fax: 201 342-1123La ur ent BOUSQUET wrote: >I am looking for a company which provides manufacturing custom rapid test > using reagents and components furnished by my laboratory. > >Emmanuelle Laurent BOUSQUET wrote: >I am looking for a company which provides manufacturing custom rapid test > using reagents and components furnished by my laboratory. > >Emmanuelle kp From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!news1.erols.com!newsfeed.internetmci.com!in2.uu.net!news.ios.com!usenet From: krisprasad Newsgroups: bionet.diagnostics Subject: Re: information about rapid tests Date: 17 Apr 1996 21:14:49 GMT Organization: Internet Online Services Lines: 17 Message-ID: <4l3n09$1v7@news.ios.com> References: <31702C42.6F33@accescyb.fr> NNTP-Posting-Host: ppp-52.ts-9.hck.idt.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) To: emmanuelle,at,Laurent,Bousquet DO you mean if we will manufacture the product to your specifications using your reagents? If yes, we would like to get some more information to see if we can manufacture your product. email:prasad@village.ios.com Vipra, Inc. Tel: 201 489-8450 Fax: 201 342-1123 kp From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Newsgroups: bionet.diagnostics Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.reston.ans.net!tank.news.pipex.net!pipex!dish.news.pipex.net!pipex!tube.news.pipex.net!pipex!lade.news.pipex.net!pipex!sasa.gov.uk!news From: odonnell@sasa.gov.uk (Kevin O'Donnell) Subject: Re: LCR Paper Abstract Organization: Scottish Agricultural Science Agency Date: Wed, 17 Apr 1996 13:53:23 GMT Message-ID: X-Newsreader: WinVN 0.91.6 X-Nntp-Posting-Host: comp128.sasa.gov.uk References: <199604171049.LAA25410@caird.scri.sari.ac.uk> Sender: news@sasa.gov.uk (Usenet) Lines: 48 In article <199604171049.LAA25410@caird.scri.sari.ac.uk>, djones@SCRI.SARI.AC.UK (D Jones) says: > I'd be really interested to see the preliminary results you >refer to. Was the comparison done on dormant tubers, or on tubers on >which dormancy had been broken? Also what were the comparative costs of >the two techniques? In my experience with molecular techniques, even when >labour costs are taken into account, the cost compared to ELISA can be >prohibitive. Seeing as ultimately the cost of any test is bourne by the >grower and farmers are notoriously 'careful with there money' this is an >important consideration. Any one else have any views? > David, You are welcome to see the preliminary results - in fact if you'd like an off-print please let me know. The work was carried out using purified virus and viral RNA rather than tuber material, however that will be the next stage. The paper reports a 10-fold increase in sensitivity over ELISA. We have since increased that to 100-fold and believe that there is further room for improvement. You are absolutely correct, IMO, to identify cost as the key criterion in agricultural diagnostics. For many purposes it just doesn't make sense at the moment to use nucleic-acid based techniques when cheaper ones do the job just as well. However, post harvest tuber testing in potatoes may be an exception, because of the high costs of the existing method. for example, greenhouse space for 6-8 weeks is a significant overhead - especially with a high throughput of samples. The grower is also inconvenienced by the long wait to discover whether his crop is of a good enough standard to be sold as seed or whether it must be sold as ware. For that reason they may be willing to pay a slightly higher price for a faster test, if given the choice. Once the method is optimised and a direct comparison made with ELISA on tests on dormant tubers, then we will be able to compare costs. All I can say at the moment is that I expect the costs to be in the same 'ballpark'. The capacity of the LCR method to dispence with the costs associated with electrophoresis willl be a help here. Kevin Dr Kevin O'Donnell Diagnostics and Molecular Biology SASA Edinburgh From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!sasa.gov.uk!mulholl From: mulholl@sasa.gov.uk (Vince Mulholland) Newsgroups: bionet.diagnostics Subject: Paper abstract Date: 17 Apr 1996 06:15:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 79 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >Some time ago, I suggested that people could post abstracts of >diagnostics papers here, in order to stimulate more on-topic >discussion. Here is an abstract from my own laboratory, of a paper >published in the Proceedings of the Diagnostics in Crop Protection >conference held in Warwick, UK earlier this month. > >STUFF DELETED > >Dr Kevin O'Donnell "Nature is not cruel, only >Diagnostics and Molecular Biology pitilessly indifferent." >SASA - Richard Dawkins >Edinburgh Here is another abstract from the same conference. Comments gratefully received - look forward to seeing any posts. --------------------------------------------------------------------- USE OF THE POLYMERASE CHAIN REACTION TO DISCRIMINATE POTATO CYST NEMATODE AT THE SPECIES LEVEL V. Mulholland1, L. Carde1, K.J. O'Donnell1, C.C. Fleming2 and T.O. Powers3 1 Diagnostics & Molecular Biology Section, SASA, East Craigs, Edinburgh EH12 8NJ, UK. 2 Dept of Applied Plant Science, DANI, Newforge Lane, Belfast, UK. 3 Dept of Plant Pathology, University of Lincoln, Nebraska, USA. Potato cyst nematode (PCN) is responsible for significant loss in the potato production of the EC. The use of PCN-contaminated land for further potato crops is dependent on the species that infests the field. Two species of PCN are endemic in the UK; Globodera pallida and Globodera rostochiensis. As there are no potato varieties fully resistant to G. pallida, land contaminated with this species of PCN can be unavailable for potato crops for a longer period. The present identification techniques include microscopic examination of the cyst contents, and the use of isoelectric focusing gels. A polymerase chain reaction (PCR) technique has been developed based on allele-specific amplification. This method amplifies a region within the internally transcribed spacer (ITS) region of the ribosomal RNA genes. A species-specific primer was designed for each species, binding to different regions of the ITS. Each of these primers is designed to mismatch with the other species at the 3' end. A third primer, which binds perfectly to both species is located downstream of the binding sites of the species-specific primers. Amplification using the Stoffel fragment of Taq DNA polymerase of G. pallida extracts gives rise to a 391 bp fragment, while G. rostochiensis produces a 238 bp fragment. Mixed populations will result in the production of both fragments. The DNA products are visualised following agarose gel electrophoresis. This technique allows identification of PCN species within 3 hours. --------------------------------------------------------------------- Vincent Mulholland Higher Scientific Officer, Diagnostics & Molecular Biology Section, Scottish Agricultural Science Agency, East Craigs, Edinburgh EH12 8NJ, U.K. E-Mail: mulholl@sasa.gov.uk Tel: (0131) 244 8845 Fax: (0131) 244 8912 From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!SCRI.SARI.AC.UK!djones From: djones@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Re: LCR Paper Abstract Date: 17 Apr 1996 03:53:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604171049.LAA25410@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net > DETECTION OF POTATO VIRUS Y USING THE LIGASE CHAIN REACTION > (LCR), IN COMBINATION WITH A MICROTITRE PLATE BASED METHOD > FOR PRODUCT DETECTION > > K J O'DONNELL, E CANNING and L G A YOUNG > Scottish Agricultural Science Agency, East Craigs, Edinburgh, EH12 8NJ, UK > email: odonnell@sasa.gov.uk > > > ABSTRACT > > The Scottish Agricultural Science Agency (SASA) carries out diagnostic > tests for viruses on potato leaf and tuber samples in support of the Scottish > Seed Potato Classification Scheme. Currently, routine detection of potato > viruses in submitted leaf samples is carried out using ELISA (enzyme-linked > immunosorbent assay). However, ELISA is not sufficiently sensitive > to consistently detect the small amounts of virus present in primary infected > tubers. In this case, the dormancy of the tubers must first be broken and then > the resulting plants tested by ELISA, a process which can take several weeks. > > In order to reduce this period we have evaluated nucleic acid amplification > techniques which can theoretically achieve the level of sensitivity necessary > for virus detection directly from tubers. In this paper we report on the > development of an assay for PVY based on the ligase chain reaction (LCR), > a method based on the ligase-mediated exponential amplification of DNA probes > specific to target DNA (or cDNA) of the pathogen. We have combined the > LCR assay with a microtitre plate based detection system which removes the > need to run electrophoresis gels to detect products of the assay. This method > has the possibility of combining the sensitivity and specificity of nucleic > acid-based techniques with the automation of ELISA. Preliminary results are > shown which compare the performance of LCR with the standard ELISA method. > > Dr Kevin O'Donnell "Nature is not cruel, only > Diagnostics and Molecular Biology pitilessly indifferent." > SASA - Richard Dawkins > Edinburgh > > Kevin. I'd be really interested to see the preliminary results you refer to. Was the comparison done on dormant tubers, or on tubers on which dormancy had been broken? Also what were the comparative costs of the two techniques? In my experience with molecular techniques, even when labour costs are taken into account, the cost compared to ELISA can be prohibitive. Seeing as ultimately the cost of any test is bourne by the grower and farmers are notoriously 'careful with there money' this is an important consideration. Any one else have any views? Dave Jones Virology SCRI Invergowrie Dundee From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!MAIL.UMU.SE!hong From: hong@MAIL.UMU.SE (Hong Qian) Newsgroups: bionet.diagnostics Subject: Looking for protein Date: 17 Apr 1996 02:34:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604170931.AA06685@mail.umu.se> NNTP-Posting-Host: net.bio.net Hello everyone out there: I am a Ph D. student working for protein structure determination by NMR method. I had finished calculating one protein structure which contains 90 amino acids. Now I am looking for a new protein. If someone has an over-expressed protein which is suitable for NMR study, I would be very happy working for it. The protein used for NMR structure calculation must fufill the following conditions: 1. Solvable at mili-molar range in water. 2. Stable at room temperature. 3. Molecular weight below 30 kD 4. If it contains more than 70 amino acids, it should be isotopic labelled by nitrogen 15 or both nitrogen-15 and carbon-13. Generally, we need around 10 mg protein for NMR study. Of course, it depends on molecular weight of protein. If anyone interest in this, please contact with me by email. Hong Qian NMR Research Group Department of Organic Chemistry Umea University S- 901 87, Umea Sweden email: hong@indro.chem.umu.se tel: 46-90-166936 fax: 46-90-138885 From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: LCR Paper Abstract Date: 17 Apr 1996 02:07:35 -0700 Organization: Scottish Agricultural Science Agency Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <9604170903.aa12318@jura.sasa.gov.uk> Reply-To: odonnell@sasa.gov.uk NNTP-Posting-Host: net.bio.net Some time ago, I suggested that people could post abstracts of diagnostics papers here, in order to stimulate more on-topic discussion. Here is an abstract from my own laboratory, of a paper publ;ished in the Proceedings of the Diagnostics in Crop Protection conference held in Warwick, UK earlier this month. DETECTION OF POTATO VIRUS Y USING THE LIGASE CHAIN REACTION (LCR), IN COMBINATION WITH A MICROTITRE PLATE BASED METHOD FOR PRODUCT DETECTION K J O'DONNELL, E CANNING and L G A YOUNG Scottish Agricultural Science Agency, East Craigs, Edinburgh, EH12 8NJ, UK email: odonnell@sasa.gov.uk ABSTRACT The Scottish Agricultural Science Agency (SASA) carries out diagnostic tests for viruses on potato leaf and tuber samples in support of the Scottish Seed Potato Classification Scheme. Currently, routine detection of potato viruses in submitted leaf samples is carried out using ELISA (enzyme-linked immunosorbent assay). However, ELISA is not sufficiently sensitive to consistently detect the small amounts of virus present in primary infected tubers. In this case, the dormancy of the tubers must first be broken and then the resulting plants tested by ELISA, a process which can take several weeks. In order to reduce this period we have evaluated nucleic acid amplification techniques which can theoretically achieve the level of sensitivity necessary for virus detection directly from tubers. In this paper we report on the development of an assay for PVY based on the ligase chain reaction (LCR), a method based on the ligase-mediated exponential amplification of DNA probes specific to target DNA (or cDNA) of the pathogen. We have combined the LCR assay with a microtitre plate based detection system which removes the need to run electrophoresis gels to detect products of the assay. This method has the possibility of combining the sensitivity and specificity of nucleic acid-based techniques with the automation of ELISA. Preliminary results are shown which compare the performance of LCR with the standard ELISA method. Dr Kevin O'Donnell "Nature is not cruel, only Diagnostics and Molecular Biology pitilessly indifferent." SASA - Richard Dawkins Edinburgh From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!sasa.gov.uk!odonnell From: odonnell@sasa.gov.uk ("Kevin O'Donnell") Newsgroups: bionet.diagnostics Subject: Newsgroup Charter Date: 17 Apr 1996 01:59:12 -0700 Organization: Scottish Agricultural Science Agency Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <9604170854.aa12042@jura.sasa.gov.uk> Reply-To: odonnell@sasa.gov.uk NNTP-Posting-Host: net.bio.net We seem to be having a run of medical problems again, so I thought that this was an opportune time to re-post the Charter of this newsgroup. I will try to do this every month or so from now on. Newsgroup Charter: The DIAGNOSTICS newsgroup (bionet.diagnostics) is a forum for discussion of problems and techniques (primarily serological or nucleic acid-based) involved in all fields of diagnostics. Subjects for discussion and comment include methodology, automation, quality assurance and other subjects of interest and concern to those developing and/or using diagnostic tests. Justification Despite the large number of scientists involved in diagnostics, there is no newsgroup devoted to this subject. The prototype DIAGNOSTICS newsgroup was set up to fill this gap and has gained enough support to justify a vote for full newsgroup status. The prototype newsgroup has included postings on the detection and identification of diseases, pathogens (animal and plant) and also diagnostic functions which do not fit into either category. This composition has helped to establish common ground (and therefore intellectual and methodological cross-fertilization) between people and laboratories. For example, the problems facing scientists developing PCR assays for Listeria are similar to those facing scientists developing PCR assays for bacterial soft rots in plants. Within the various disciplines there are common problems regarding methods, immunogens, cross-reactivity, automation, materials, quality assurance and other issues that are not recognized by those using similar techniques for other purposes. The DIAGNOSTICS newsgroup will address and help solve these problems by providing a forum for their discussion between individuals of various experience and disciplines. Dr Kevin O'Donnell "Nature is not cruel, only Diagnostics and Molecular Biology pitilessly indifferent." SASA - Richard Dawkins Edinburgh From owner-diagnostics@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!nntp-trd.UNINETT.no!nntp.uio.no!news.cais.net!news1.erols.com!newsfeed.internetmci.com!in2.uu.net!news.ios.com!usenet From: krisprasad Newsgroups: bionet.diagnostics Subject: Re: information about rapid tests Date: 17 Apr 1996 21:17:20 GMT Organization: Internet Online Services Lines: 29 Message-ID: <4l3n50$1v7@news.ios.com> References: <31702C42.6F33@accescyb.fr> NNTP-Posting-Host: ppp-52.ts-9.hck.idt.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2 (Windows; U; 16bit) To: lbel@accescyb.fr DO you mean if we will manufacture the product to your specifications using your reagents? If yes, we would like to get some more information to see if we can manufacture your product. email:prasad@village.ios.com Vipra, Inc. Tel: 201 489-8450 Fax: 201 342-1123La ur ent BOUSQUET wrote: >I am looking for a company which provides manufacturing custom rapid test > using reagents and components furnished by my laboratory. > >Emmanuelle Laurent BOUSQUET wrote: >I am looking for a company which provides manufacturing custom rapid test > using reagents and components furnished by my laboratory. > >Emmanuelle kp From owner-diagnostics@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!jussieu.fr!infobiogen.fr!pasteur.fr!univ-lyon1.fr!citi2.fr!camoin.cochin.inserm.fr!user From: brydon@icgm.cochin.inserm.fr (lena brydon) Newsgroups: bionet.diagnostics Subject: Review Antibodies As Tools Date: Thu, 18 Apr 1996 17:41:59 +0200 Organization: icgm cnrs upr415 Lines: 17 Message-ID: NNTP-Posting-Host: camoin.cochin.inserm.fr X-Newsreader: Yet Another NewsWatcher F2.0 Dear Netters, I plan to compile a review of 1. existing methods or novel ideas for raising specific monoclonal and polyclonal antibodies and 2. the potential uses of such antibodies. I would be very grateful for any information/ideas you have regarding this subject area. The results of this inquiry will be sent to this news group later. If anyone has any papers recently published or in Press regarding this subject and are willing to send me re-prints, I will of course quote these authors in the review ( if so desired.) Lena Brydon e mail: brydon@icgm.cochin.inserm.fr From owner-diagnostics@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!IRAPUATO.IRA.CINVESTAV.MX!jpms From: jpms@IRAPUATO.IRA.CINVESTAV.MX (Juan Pablo Martinez-Soriano) Newsgroups: bionet.diagnostics Subject: Abour PCR sensitivity Date: 18 Apr 1996 15:47:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net In a recent discussion, Kevin mentioned: > The work was carried out using purified >virus and viral RNA rather than tuber material, however that will be the >next stage. The paper reports a 10-fold increase in sensitivity over >ELISA. We have since increased that to 100-fold and believe that there is >further room for improvement. In recent experiments made at our lab, we have improved RT-PCR sensitivity up to 1000-fold when compared with DAS-ELISA trying to detect Citrus Tristeza Virus from FIELD samples. We have been using 2 sets of primers (four oligos) that amplify two different regions of the viral genome but yield same size fragments. We have seen that sensitivity goes even way up if we combine ELISA and RT-PCR. That is, RT-PCR made after ELISA. >For many purposes it just doesn't make sense at >the moment to use nucleic-acid based techniques when cheaper ones do >the job just as well. > >However, post harvest tuber testing in potatoes may be an exception, Well, you said it well. It depends on what is the bottom end of the detection. In Mexico we are doing a massive survey where thousands of trees need to be tested for CTV. It would be crazy to even thing to try RT-PCR on each of them. We pool 5 to 10 samples (each coming from one tree) and do ELISA on them, which was fine for some time. Suddenly, number of samples and time became critical and we needed more fast and sensitive tools. We know now that we can pool 100 (or more) samples and detect the presence of one infected tree (even if 99 are healthy) with our RT-PCR. You can easily see that cost is divided by 100 (or more) making the PCR test (per tree) way cheaper than ELISA. Do not ask me why we do not do hybridisations. See, potaoes are not exception anymore. Mexican citrus are along. Best regards from sunny Mexico -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' Juan Pablo Martinez-Soriano jpms@irapuato.ira.cinvestav.mx CINVESTAV Unidad Irapuato Apdo. postal 629 Phone [01152] (462) 51600 Irapuato, Gto, MEXICO Fax [01152] (462) 45996 -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' *thanks to Gerard R Lazo From owner-diagnostics@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Dr. S. K. Rana" Newsgroups: bionet.diagnostics Subject: HELP !! Diagnosis of BLUETONGUE infection Date: 19 Apr 1996 10:53:10 +0100 Organization: National Dairy Development Board Lines: 28 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4l7nq6$5j5@mserv1.dl.ac.uk> X-Confirm-Reading-To: "Dr. S. K. Rana" Original-To: diagnost@dl.ac.uk, microbio@dl.ac.uk.virology X-Pmrqc: 1 Hello every one, I am not a regular member of this group, but am sure that some of you might be able to give the information or the contact address for the information, I am seeking. 1. Information on use of PCR for the detection of Bluetongue virus in animals and their products. 2. Production of antigen for use in Agar Gel Immunodiffusion (AGID) test for diagnosis of Bluetongue. Please send me the information on the following address : Thanks in anticipation. SK Rana ********************************************************************* Dr. SK Rana, Scientist-II (BT) National Dairy Development Board, ANAND, PIN - 388 001, INDIA Fax : 91-2692-40156 or 91-2692-40165 E-mail : samirkr@anand.nddb.ernet.in ********************************************************************* From owner-diagnostics@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!uwyo.edu!WCWilson From: WCWilson@uwyo.edu (William C. Wilson) Newsgroups: bionet.diagnostics Subject: Bluetongue Diagnostics Date: 19 Apr 1996 06:20:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 80 Sender: daemon@net.bio.net Distribution: world Message-ID: <1996Apr19.070537.1036.81854@jackrabbit.uwyo.edu> NNTP-Posting-Host: net.bio.net Several labs including mine have published procedures for detection of bluetongue and the related epizootic hemorrhagic disease viruses. Attached is a partial listing of available references concerning BTV PCR. There are also several published protocols for detection of African horse sickness that I didn't include for the sake of space. Reference List 1. Akita, G. Y.; Chinsangaram, J.; Osburn, B. I.; Ianconescu, M., and Kaufman, R. Detection of bluetongue virus serogroup by polymerase chain reaction. J. Vet. Diagn. Invest. 1992; 4:400-405. 2. Akita, G. Y.; Glenn, J.; Castro, A. E., and Osburn, B. I. Detection of bluetongue virus in clinical samples by polymerase chain reaction. J. Vet. Diagn. Invest. 1993; 5:154-158. 3. Aradaib, I. E. Akita G. Y. Osburn B. I. Detection of epizootic hemorrhagic disease virus serotypes 1 and 2 in cell culture and clinical samples using polymerase chain reaction. J. Vet. Diagn. Invest. 1994; 6:143-147. 4. Aradaib, I. E.; Wilson, W. C.; Cheney, I. W., and Osburn, B. I. Application of PCR for specific-identification of epizootic hemorrhagic disease virus serotype 2. J. Vet. Diagn. Invest. 1995; 7:388-392. 5. Aradaib, I. E.; Wilson, W. C.; Cheney, I. W.; Pearson, J. E., and Osburn, B. I. Application of PCR for specific identification of epizootic hemorrhagic disease virus serotype 2. Journal of Veterinary Diagnostic Investigation. 1995 Jul; 7(3):388-392. 6. Dangler, C. A.; de Mattos, C. A.; de Mattos, C. C., and Osburn, B. I. Identifying bluetongue virus ribonucleic acid sequences by the polymerase chain reaction. J. Virol. Meth. 1990; 28:281-292. 7. Katz, J. B.; Alstad, A. D.; Gustafson, G. A., and Moser, K. M. Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid. J. Clin. Microbiol. 1993; 31(11):3028-3030. 8. Katz, J. B.; Gustafson, G. A.; Alstad, A. D.; Adler, K. A., and Moser, K. M. Colorimetric Diagnosis of Prolonged Bluetongue Viremia in Sheep, Using an Enzyme-Linked Oligonucleotide Sorbent Assay of Amplified Viral Nucleic Acids. Amer. J. Vet. Res. 1993; 54(12):2021-2026. 9. ---. Colorimetric diagnosis of prolonged bluetongue viremia using an enzyme-linked oligonucleotide sorbent assay of amplified viral nucleic acids. J. Vet. Diag. Invest. 1993(In Press). 10. Maclachlan, N. J.; Nunamaker, R. A.; Katz, J. B.; Sawyer, M. M.; Akita, G. Y.; Osburn, B. I., and Tabachnick, W. J. Detection of bluetongue virus in the blood of inoculated calves: Comparison of virus isolation, PCR assay, and in vitro feeding of Culicoides variipennis. Arch. Virol. 1994; 136(1-2):1-8. 11. McColl, KA and Gould, AR. Detection and characterisation of bluetongue virus using the polymerase chain reaction. Virus Res. 1991; 21(1):19-34. 12. Mecham, J. O. Wilson W. C. Strategies for improved bluetongue diagnostic strategies for improved bluetongue diagnostics. Proc. U.S. Anim. Health Assoc. 1994; 98:43-50. 13. Wade-Evans A.M.; Mertens, PP, and Bostock, CJ. Development of the polymerase chain reaction for the detection of bluetongue virus in tissue samples. J. Virol. Methods. 1990; 30(1):15-24. 14. Wechsler, SJ; Austin, KJ, and Wilson, WC. Limits of detection of bluetongue virus with different assay systems. J. Vet. Diagn. Invest. 1990; 2(2):103-6. 15. Wilson, W. C. Development of nested-PCR tests based on sequence analysis of epizootic hemorrhagic disease viruses non-structural protein 1 (NS1). Virus Res. 1994; 31:357-365. 16. Wilson, W. C. and Chase, C. C. L. Nested and Multiplex Polymerase Chain Reactions for the Identification of Bluetongue Virus Infection in the Biting Midge, Culicoides-variipennis. J. Virol. Meth. 1993; 45(1):39-47. Hope this is helpful. **************************************************************************** William C. Wilson USDA-ARS Arthropod-borne Animal Diseases Research Lab. Laramie, WY 82071-3965 Phone : (307)766-3622 Fax: (307)766-3500 E-Mail: wcwilson@uwyo.edu **************************************************************************** From owner-diagnostics@net.bio.net Fri Apr 19 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in1.uu.net!news.zNET.net!news From: Kristen Copeland Newsgroups: bionet.diagnostics Subject: Adenovirus ELISA Date: 20 Apr 1996 00:23:01 GMT Organization: kc.net Consulting Lines: 9 Message-ID: <4l9ap5$g6s@news> NNTP-Posting-Host: sv17.znet.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1 (Macintosh; U; PPC) X-URL: news:bionet.diagnostics I am looking for both monoclonal and polyclonal antibodies to Adenovirus ( mainly type 40 and 41) for the development of an ELISA. If anyone can help, please e-mail lmdlabs@lmdlabs.com. If you would like more information on my company, check out our web page: http://www.lmdlabs.com/ From owner-diagnostics@net.bio.net Sun Apr 21 23:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!sunsite.doc.ic.ac.uk!agate!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!howland.reston.ans.net!EU.net!sun4nl!Inter.NL.net!usenet From: "F.Lafort" Newsgroups: bionet.diagnostics Subject: development and procurement of blood and safe blood products Date: Sun, 21 Apr 1996 12:38:00 -0700 Organization: Lafort Public Affairs Lines: 47 Message-ID: <317A8E98.78F3@inter.nl.net> NNTP-Posting-Host: gd99-18.gouda.nl.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) Pre-announcement ================ NATO CONFERENCE ON EFFICIENT AND SAFE BLOOD SUPPLY From 5-8 May 1996 The Netherlands is host of the 4th NATO Military and Civil Blood Conference. This international conference (venue: Netherlands Defense College, Ypenburg) will focus on scientific and logistic aspects concerning the development and procurement of blood and safe blood products. This conference will be the 4th in a series of meetings, the first of which was held in Aldershot, UK in 1990, followed by Brussels, Belgium in 1992 and Istanbul, Turkey in 1994. In the framework of the programme "Partnership for Peace" participants from Mid- and East European Nations will join this conference. Objectives of the 4th NATO Military and Civil Blood Conference 1) Discussion on the latest developments in the collection, preparation and storage of blood and blood products, including new techniques on viral inactivation. 2)Discussion on new developments in artificial oxygen carriers and the use in military and humanitarian operations. 3)Discussion on the logistics of blood in extreme climatologic circumstances and its clinical implications. 4)Discussion on transfusion medical aspects in NATO and UN Peace Keeping and Enforcing operations, including the use of deep frozen blood products. 5) Formulation of recommendations for standardisation, validation and certification of military and civil Blood Banks. 6) Formulation of ideas and plans for the exchange of blood and blood products amongst Allied Nations in areas of operation. This conference is being organised by the Joint Medical Committee of NATO in close cooperation with the Military Medical Policy of the Ministry of Defense, the Military Blood Bank, The Federation of Netherlands Red Cross Blood Banks and the Central Laboratory of the Netherlands Red Cross Blood Transfusion Service. More information: prof.dr. E.J. Ruitenberg, chairman 'Scientific Committee, c/o CLB, Plesmanlaan 125, 1066 CX AMSTERDAM, tel. 020-512.3224 or mr. E.G.J. Somer, NATO, Brussels, tel. 00.32.2.707.4069 E Mail: t.evers@epfa.nl Website: http://www.cyber.nl/epfa/ From owner-diagnostics@net.bio.net Mon Apr 22 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!surfnet.nl!howland.reston.ans.net!torn!nott!cunews!freenet-news.carleton.ca!FreeNet.Carleton.CA!bc005 From: bc005@FreeNet.Carleton.CA (ZX Lei) Newsgroups: bionet.diagnostics Subject: Re: help wanted for a ill girl Date: 23 Apr 1996 17:02:03 GMT Organization: The National Capital FreeNet Lines: 107 Sender: bc005@freenet2.carleton.ca (ZX Lei) Message-ID: <4lj2eb$non@freenet-news.carleton.ca> References: <4lhje9$ru@wumpus.its.uow.edu.au> Reply-To: bc005@FreeNet.Carleton.CA (ZX Lei) NNTP-Posting-Host: freenet2.carleton.ca Xinyang Li (xyl@wumpus.its.uow.edu.au) writes: > From: chang@neu.edu.cn (Chang Guiran) > Message-Id: <9604190226.AA09250@neu.edu.cn> > To: neu-l@uwalpha.uwinnipeg.ca > Subject: FW: Letter for asking for advice for a lovely little Chinese girl > > Dear Friends, > > Please help to distribute the following letter. Thanks. > > > > ------------------------------------------------------------- > Letter for asking for advice for a lovely little Chinese girl > ------------------------------------------------------------- > > This is a letter crying out for help for an innocent and clever girl only six > > years old, my daughter, who's name is Zhao Chengxi. > > She has suffered from a kind of very rare and terrible illness for more than > two years. I have taken her to many hospitals and she has been treated with > many treatments,up to now the illness she suffered from is diagnosed as > "Allergic Cutaneous Vasculitis", but because of the rareness of this kindof > illness, it is very difficult to cure this illness. Now she still lives in a > terrible situation full of suffering. > > Following are the details about the treatments of my daughter : > --------------------------------------------------------------------------- >>From February 1994 onwards, my daughter began to have a fever, her > temperature usually reached over thirty-nine degrees centigrade. Meantime, > some painless rashes appeared on the skin.Afterbeingtreated with interferon, > her temperature dropped to normal, the rashes also disappeared. > > But from June 1994 onwards, some itching papules 1.0spuare centimeters > in size appeared on the skin of my daughter, and there are blisters > in the center of these papules.Her temperature reached to forty > degrees centigrade, no shiver,normal antipyretics were used,but they > are useless, high temperature continued.Above symptoms kept appearing > repeatedly afterwards. > >>From August 12 1994 to September 22 1994, I took my daughter to the > subsidiary hospital of Chinese Medical University in Shenyang. Througha > systematic examination, the doctors there diagnosed this illness as > "Allergic Cutaneous Vasculitis". The main medicine the doctors used is > "Dexamethasone", a kind of hormone. Indeed this kind of medicine has > some curative effect. The daily dose of this kind of oral medicine is > thirty milligrams at the beginning.But after the daily dose was reduced > gradually, the illness state of my daughter got bad again,until July > 1995, it became very terrible and dangerous, her > hands#027#$)A#014#!"#015#feet and > limbs began to appearoedema,her temperature reached forty degrees centigrade. > > Moreover the side effects of hormone are very clear:My daughter,a very > slender girl, was getting fatter than before;her kidneys and other visceras > were harmed to some extent. > > In order to carry out more precise diagnosis and better treatment, > from July 17 1995 to September 17 1995, I took my daughter to Beijing, > We lived in the subsidiary Beijing Children's Hospital ofBeijing Medical > University, the best Children's Hospital in China . > > During the time in Beijing Children's Hospital, systematic and strict > examination aboutblood#014#!"#015#urine#014#!"#015#dung and others showed > that the palpitation > and the breath of lung are normal, but the amount of white cell exceed > normal and the liver is bigger than normal. Especially through the > skin biopsy, the dortors diagnosed this illness as "Allergic Cutaneous > Vasculitis", according to above systematic examination especially the > skin biopsy this diagnosis shold be precise, the main medicine is still > hormone. Through two monthes' treatment,theillness state of my daughter > was restrained to some extent.On September17we left Beijing Children's > Hospital. > >>From September 17 to now, the illness state of my daughter began to > become bad again, her hands#014#!"#015#feet#014#!"#015#limbs and face > became oedematous, > her temperature also reached forty degrees centigrade.That means the > original syptoms of this illness appeared again, in anther word, my > daughter has not been cured well and completely,for the illness > "Allergic Cutaneous Vasculitis",better effective treatment has not been > found yet in China now. > > Now I really feel disappointed, I really worry about losing my lovely > daughter. > So now I send this news by means of the internet asking for advice from you, > it is the second time to sound this news (two monthes ago I sounded the first > > news ). > > I wish fromthe bottom of my heart that you provide the best treatment > to this illness _____" Allergic Cutaneous Vasculitis " try your utmost. I am > sure you have this ability. > Thank you > > Any information please send to : > huqiao@mail.neu.edu.cn > > Sincerely yours, > Zhao lin > -- From owner-diagnostics@net.bio.net Tue Apr 23 23:00:00 1996 Path: biosci!SCRI.SARI.AC.UK!djones From: djones@SCRI.SARI.AC.UK (D Jones) Newsgroups: bionet.diagnostics Subject: Re: Abour PCR sensitivity Date: 24 Apr 1996 04:55:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604241150.MAA22441@caird.scri.sari.ac.uk> NNTP-Posting-Host: net.bio.net Juan Pablo Martinez-Soriano replied to Kevin's earlier message > > > The work was carried out using purified > >virus and viral RNA rather than tuber material, however that will be the > >next stage. The paper reports a 10-fold increase in sensitivity over > >ELISA. We have since increased that to 100-fold and believe that there is > >further room for improvement. > > In recent experiments made at our lab, we have improved RT-PCR sensitivity > up to 1000-fold when compared with DAS-ELISA trying to detect Citrus > Tristeza Virus from FIELD samples. We have been using 2 sets of primers > (four oligos) that amplify two different regions of the viral genome but > yield same size fragments. > We have seen that sensitivity goes even way up if we combine ELISA and > RT-PCR. That is, RT-PCR made after ELISA. > > >For many purposes it just doesn't make sense at > >the moment to use nucleic-acid based techniques when cheaper ones do > >the job just as well. > > > >However, post harvest tuber testing in potatoes may be an exception, > > Well, you said it well. It depends on what is the bottom end of the > detection. In Mexico we are doing a massive survey where thousands of trees > need to be tested for CTV. It would be crazy to even thing to try RT-PCR on > each of them. We pool 5 to 10 samples (each coming from one tree) and do > ELISA on them, which was fine for some time. Suddenly, number of samples > and time became critical and we needed more fast and sensitive tools. We > know now that we can pool 100 (or more) samples and detect the presence of > one infected tree (even if 99 are healthy) with our RT-PCR. You can easily > see that cost is divided by 100 (or more) making the PCR test (per tree) > way cheaper than ELISA. Do not ask me why we do not do hybridisations. > > See, potaoes are not exception anymore. Mexican citrus are along. > > Best regards from sunny Mexico > I wish it was sunny here. Greetings from a very wet and rainy Scotland! There is a slight difference between potatoes and Citrus fruits. As I see it, if I've understood your message correctly, you are only looking for the presence or absence of the virus. The situation in potatoes is complicated by the seed potato classification scheme, certain levels of virus are allowable in a crop depending on what grade of seed it is. At the highest grade in which no virus is allowed then I can see that pooling samples and using PCR/LCR to detect virus would be preferable to using a less sensitive ELISA, if the costs are comparable. However at lower grades of seed in which levels af virus are allowable at 0.5% or higher, how does one quantify a pooled PCR reaction? present ELISA techniques approach 80-90% accuracy (my figures) and give an absolute figure on the level infection in the crop. I'm sure a statistician will tell me that it is possible to devise a sampling method that could correct for pooled samples, but I need to be convinced! I do believe however that as time goes on and the cost of PCR goes down, it will be possible to test individual samples, though I think that this is some way off yet. Regards Dave D.A.C. Jones SCRI Invergowrie Dundee DD2 5DA From owner-diagnostics@net.bio.net Wed Apr 24 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!sgigate.sgi.com!news1.best.com!news.aimnet.com!ns1.aplatform.com!ns2.mainstreet.net!bug.rahul.net!rahul.net!a2i!samba.rahul.net!rahul.net!a2i!news.PBI.net!decwrl!tribune.usask.ca!rover.ucs.ualberta.ca!unixg.ubc.ca!news!wjia From: wjia@unixg.ubc.ca (William Jia) Newsgroups: bionet.diagnostics Subject: PCR diagnositic technology Followup-To: bionet.diagnostics Date: Wed, 24 Apr 96 20:14:46 GMT Organization: The University of British Columbia Lines: 6 Message-ID: NNTP-Posting-Host: extra.ecc.ubc.ca X-Newsreader: VersaTerm Link v1.1.1 Hi, I wonder if anybody knows whether a PCR based diagnostic technology is availabe for screening large number of samples in a reasonable time (say 10 hours)? Please email to this account. Thanks in advance. William From owner-diagnostics@net.bio.net Thu Apr 25 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!usenet.eel.ufl.edu!arclight.uoregon.edu!news.bc.net!news.mindlink.net!line079.nwm.mindlink.net!Innovatek From: Innovatek@mindlink.bc.ca (Innovatek Medical Inc.) Newsgroups: bionet.diagnostics Subject: Latex reagents wanted Date: Fri, 26 Apr 1996 08:56:19 GMT Organization: MIND LINK! - British Columbia, Canada Lines: 15 Message-ID: NNTP-Posting-Host: line079.nwm.mindlink.net X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] We are interested in buying later reagents, ASO, CRP, AFP, mono, RF, Staph, and SLE in bulk. Not interested in pregnancy test at the moment. Preferably has US FDA 510(k) but not absolutely necessary. Contact: Mr. King F. Hui Innovatek Medical Inc. 150 - 13500 Maycrest Way, Richmond, B.C., Canada. V6V 2N8 Tel: (604) 278-8886 Fax: (604) 278-7999 E-mail: INNOVATEK@mindlink.bc.ca From owner-diagnostics@net.bio.net Thu Apr 25 23:00:00 1996 Path: biosci!daresbury!sunsite.doc.ic.ac.uk!agate!cgl!itssrv1.ucsf.edu!itsa.ucsf.edu!bgold From: bgold@itsa.ucsf.edu (Bert Gold) Newsgroups: bionet.diagnostics Subject: Re: PCR diagnositic technology Date: 26 Apr 1996 18:54:31 GMT Organization: UCSF, ITS Lines: 16 Message-ID: <4lr657$u80@itssrv1.ucsf.edu> References: NNTP-Posting-Host: itsa.ucsf.edu X-Newsreader: TIN [version 1.2 PL2] William Jia (wjia@unixg.ubc.ca) wrote: : Hi, : I wonder if anybody knows whether a PCR based diagnostic technology is : availabe for screening large number of samples in a reasonable time (say 10 : hours)? Please email to this account. Thanks in advance. : William To everything there is a season, and a time for every purpose under heaven. - Ecclesiastes For what purpose do aim to use PCR? Bert Gold San Francisco From owner-diagnostics@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!UVA.PCMAIL.VIRGINIA.EDU!mgk2r From: mgk2r@UVA.PCMAIL.VIRGINIA.EDU ("Michael G. Kurilla") Newsgroups: bionet.diagnostics Subject: Re: PCR diagnositic technology Date: 26 Apr 1996 19:44:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604262108.RAA17550@uva.pcmail.Virginia.EDU> NNTP-Posting-Host: net.bio.net On Apr 24, 11:19pm, William Jia wrote: > Subject: PCR diagnositic technology > Hi, > I wonder if anybody knows whether a PCR based diagnostic technology is > availabe for screening large number of samples in a reasonable time (say 10 > hours)? Please email to this account. Thanks in advance. > > William > > -- End of excerpt from William Jia One method is to use a square grid setup. This only works if you have good sensitivity and expect a low positivity rate. The basic method is as follows: . . . . . . . . . . a . . . . . . . . . . b . . . . . . . . . . c . . . . . . . . . . d . . . . . . . . . . e . . . . . * . . . . f . . . . . . . . . . g . . . . . . . . . . h . . . . . . . . . . i . . . . . . . . . . j 1 2 3 4 5 6 7 8 9 10 Assume each dot is a sample. Run twenty PCR reactions mixing all the samples in each row (a, b, etc.) and each column (1, 2, etc.). If you know you can detect your positive when diluted 1:10, then it's fine. All you need to do is then determine which positive rows and columns match up and you've found your positive sample. In the above example, the asterisk is a positive, so in this case only row f and column 6 would yield positive results. In this case, you would perform 20 PCRs instead of 100 and only need to comfirm the positive. Keep in mind that this only works if you expect few positives. I have seen this method in action to isolate YAC clones from a chromosome library. In that case, grid 19 X 19 were used (38 PCRs for screening 361 different clones) for about a 10 fold efficiency. It seems that once the sample number gets large, the hands on time is greatly increased. This method reduces the number of PCRs to screen a large sample. Mike Kurilla From owner-diagnostics@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!IRAPUATO.IRA.CINVESTAV.MX!jpms From: jpms@IRAPUATO.IRA.CINVESTAV.MX (Juan Pablo Martinez-Soriano) Newsgroups: bionet.diagnostics Subject: Symposium on Brucellosis Date: 26 Apr 1996 19:55:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The Universidad Autonoma del Estado de Mexico (Mexico) is organising an International Symposium on Brucellosis. The meeting will be held in Toluca, Mexico on June 12-14, 1996. Oral and poster presentations are welcome. If you are interested, please access the following WWW page: http://ccr.dsi.uanl.mx/~jpmtz/symposium.htm Please distribute this announcement to any interested people. Thanks in advance. Greetings from Mexico Disclaimer: I am not a member of the organiser committee but I could contact you with the right people there. -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' Juan Pablo Martinez-Soriano jpms@irapuato.ira.cinvestav.mx CINVESTAV Unidad Irapuato Apdo. postal 629 Phone [01152] (462) 51600 Irapuato, Gto, MEXICO Fax [01152] (462) 45996 -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' *thanks to Gerard R Lazo From owner-diagnostics@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!IRAPUATO.IRA.CINVESTAV.MX!jpms From: jpms@IRAPUATO.IRA.CINVESTAV.MX (Juan Pablo Martinez-Soriano) Newsgroups: bionet.diagnostics Subject: More on Citrus Date: 26 Apr 1996 19:56:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net On 24 Apr 1996 04:55:03 -0700, D Jones said: > There is a slight difference between potatoes and Citrus fruits. >As I see it, if I've understood your message correctly, you are only >looking for the presence or absence of the virus. Right, citrus botanical seeds are almost useless. Desirable varieties are multiplied by grafting them on roostocks. Some strains of Citrus Tristeza Virus induce a dramatic hypersensitive reaction right at the graft union that eventually kills the tree (when the roostock is the sour orange). > I do believe however that as time goes on and the cost of PCR >goes down, it will be possible to test individual samples, though I >think that this is some way off yet. Not really, time is now in many areas of diagnosis. Just ask the MDs and the thousands of human patients. But yes I know, your point is on plants. Well, we use it while testing for clean material, either it is been exported or imported, in our plant health certification programs and so on. Regards from Mexico -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' Juan Pablo Martinez-Soriano jpms@irapuato.ira.cinvestav.mx CINVESTAV Unidad Irapuato Apdo. postal 629 Phone [01152] (462) 51600 Irapuato, Gto, MEXICO Fax [01152] (462) 45996 -. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. .-. ||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /|||X|||\ /||| |/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ \|||X|||/ ' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' `-' *thanks to Gerard R Lazo From owner-diagnostics@net.bio.net Fri Apr 26 23:00:00 1996 Path: biosci!IC.NET!ecophys From: ecophys@IC.NET (Thomas McKarns) Newsgroups: bionet.diagnostics Subject: Nitric oxide Date: 27 Apr 1996 12:06:56 -0700 Organization: ECO PHYSICS, INC. Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Research into use of Nitric Oxide (NO) measurement as a diagnostic tool for sepsis, asthma and other respiratory conditions. In Brussels ECO PHSYICS displayed a prototype of a new research instrument combining fast (0.1 s), precise measure of exhaled breath flow, volume and composition with very accurate (low ppb), fast (0.2 s) determination of nitric oxide. Contact: Tom McKarns President, ECO PHYSICS, INC. Ann Arbor, MI USA ecophys@ic.net http://ic.net/~ecophys Tel: 1-313-998-1600 From owner-diagnostics@net.bio.net Sun Apr 28 23:00:00 1996 Path: biosci!sasa.gov.uk!browning From: browning@sasa.gov.uk (Isla Browning) Newsgroups: bionet.diagnostics Subject: virus detection by chemiluminescence Date: 29 Apr 1996 10:06:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi! I would be grateful to hear from anyone who has tried Western Blotting using a Chemiluminescence Reagent (eg DuPont RENAISSANCE) for detection of viruses particularly those of potato. I am interested in the sensitivity found compared with other antibody based techniques and any practical problems. ISLA BROWNING SASA Edinburgh From owner-diagnostics@net.bio.net Mon Apr 29 23:00:00 1996 Path: biosci!AVA.BCC.ORST.EDU!martinrr From: martinrr@AVA.BCC.ORST.EDU (Robert_R Martin) Newsgroups: bionet.diagnostics Subject: (none) Date: 30 Apr 1996 11:56:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe bionet.diagnostics From owner-diagnostics@net.bio.net Mon Apr 29 23:00:00 1996 Path: biosci!sasa.gov.uk!browning From: browning@sasa.gov.uk (Isla Browning) Newsgroups: bionet.diagnostics Subject: PVYNTN Date: 30 Apr 1996 09:11:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Potato virus experts! We have not yet encountered PVYNTN in Scotland as yet but I would be most grateful to find out about symptom expression in the haulm of infected cultivars. Is there variability depending on cultivar? Can the symptom be expressed as a mild mosaic? Isla Browning Diagnostics and Molecular Biology Section SASA Edinburgh