From owner-diagnostics@net.bio.net Sun Sep 01 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Technogoddess Newsgroups: bionet.diagnostics Subject: Re: Saliva collection Date: 2 Sep 1996 01:34:39 -0700 Organization: Laboratory Technology Resources Lines: 34 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50e66v$3p2@net.bio.net> NNTP-Posting-Host: net.bio.net Dr Dave Smart wrote: > > I have seen an advert somewhere for saliva collection devices > but can't remember where. > I am interested in doing some research on saliva. > Could anyone tell me > 1: Why a collection device? Why not (to put it crudely) just > spit into a tube? > > 2: Where I can get saliva collection devices (once convinced I > need them). > > Many thanks > Dr Dave Smart Dr. Smart. There are several devices available, it is hard to recommend any particular one without knowing what kind of testing you are doing. Do you need a preservative or will it interfere with testing? Are you doing quantitative or qualitative testing? Are you looking for antibodies? What volume of specimen do you require? Are you doing drug testing? A couple of companies are Sarstedt and Epitope but there are others. With more details maybe I can make a specific recommendation. Gwen -- ++++++++++++++++++++++++++++++++++++ Gwen E. Sprague, CLS(NCA), CPC Laboratory Technology Resources "Offering solutions to help your laboratory work smarter not harder" ++++++++++++++++++++++++++++++++++++ From owner-diagnostics@net.bio.net Sun Sep 01 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Debbie Mantzaris Newsgroups: bionet.diagnostics Subject: question on tissue culture Date: 2 Sep 1996 01:46:04 -0700 Organization: Monash University Lines: 28 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50e6sc$4lq@net.bio.net> NNTP-Posting-Host: net.bio.net I wonder if you might be able to help me locate any suppliers who commercially distribute rat or rabbit gastric mucosal cells. I am particularly interested in rat mucosal cells. I would prefer buying these cells if they are available since it would save a lot of time. If you are aware of any suppliers or know of anyone who could help me could you please send me a forwarding address. I look forward to hearing from you soon. Yours sincerely, Debbie Mantzaris ------------------------------------------------- Debbie Mantzaris (postgraduate student) Department of Surgery, Monash Medical School, Alfred Hospital, Ph: 9276 2626 Fax: 9510 3365 email: Debbie@Alfred.Med.Monash.edu.Au ------------------------------------------------- From owner-diagnostics@net.bio.net Mon Sep 02 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Leigh Newsgroups: bionet.diagnostics Subject: Making Antibodies Let Go Date: 3 Sep 1996 01:24:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50gq0a$k91@net.bio.net> NNTP-Posting-Host: net.bio.net Making Antibodies Let Go If one is collecting cells by antibody binding, for instance, on a solid surface, how does one recover the cells intact? One idea is to use mechanical energy to rip the cells off the beads or solid surfaces. It would seem that this might rupture significant numbers of cell membranes. Other suggestions have been to let the cells multiply and daughter cells will grow free of the solid support. In the case of molecules I suppose it is even more important to recover the molecules whole since it will be very difficult to rip these off the solid surface without destroying them. Please share your tips for ways to make antibodies release antigens- whole molecules or cells. oooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo Leigh Bangs, aka "The Particle Doctor(TM)"; leigh@bangslabs.com Bangs Laboratories, Inc., 979 Keystone Way, Carmel, IN 46032-2823 USA Tel: 317-844-7176 Fax: 317-575-8801 "The Microsphere Zone" [Web Site: http://www.bangslabs.com/blab] oooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo From owner-diagnostics@net.bio.net Mon Sep 02 23:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Sep 1996 02:55:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50gvb6$sk6@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Tue Sep 03 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Michel Masson Newsgroups: bionet.diagnostics Subject: recherchons biologiste Date: 4 Sep 1996 01:21:01 -0700 Organization: sprl M.Masson Lines: 3 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50je5d$qab@net.bio.net> NNTP-Posting-Host: net.bio.net Recherchons biologiste pour PMA : FIVETE, ICSI ... Me contacter s; v.p par email Michel.Masson@ping.be From owner-diagnostics@net.bio.net Tue Sep 03 23:00:00 1996 Path: biosci!biosci!not-for-mail From: jim Newsgroups: bionet.diagnostics Subject: Re: micro BUN and creatinin test Date: 4 Sep 1996 01:19:02 -0700 Organization: National Naval Medical Center Lines: 28 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <50je1m$q07@net.bio.net> NNTP-Posting-Host: net.bio.net > Almost any test that can be done on an analyzer (Hitachi-Olympus-etc) > can be converted to be run on a microplate. I've converted many using > the same reagents we were using on our large analyzers. You would have > to hand pipet the samples but otherwise all you need is a plate reader, > pipets to add the reagents and samples, possibly (but not required) a > plate incubator. Most tests will require 3-15 ul of serum (but that can > be adjusted too) which can be harvested from blood collected in > cappillary tubes. BUN and Creatinine would be easy since both can be run > as endpoint tests. Thanks. I need something that I can do in a regular basic science (microbiology) lab. the analyzer availible is a Kodak and I don't have the ability to change it at all. Any ideas? From owner-diagnostics@net.bio.net Mon Sep 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: larel Newsgroups: bionet.diagnostics Subject: Gen-Probe Combination Test Date: 10 Sep 1996 15:01:09 -0700 Organization: Foothill Net Lines: 3 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <3235A7B8.43FB@foothill.net> NNTP-Posting-Host: net.bio.net Does anyone know if the Gen-Probe Chlamydia and Gonorreha Combination Test is performing well in asymptomatic cases? From owner-diagnostics@net.bio.net Mon Sep 09 23:00:00 1996 Path: biosci!biosci!not-for-mail From: bratliff@jax-inter.net (bill ratliff) Newsgroups: bionet.diagnostics Subject: OSHA and Managed Care Plan Date: 10 Sep 1996 14:57:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <511r94$sem@news0-alterdial.uu.net> NNTP-Posting-Host: net.bio.net Managed care plans have started checking for physician compliance with OSHA Bloodborne Pathogen Standard. Plans are looking for docs compliance eith the annual education, exposure control plan and Hepatitis B vaccination. All physician practices must comply by law, or face stiff penalties (up to $7,000.00!!!!!). However, there are some exemptions for some of the doctors depending on the legal set-up of your practice. I was referred to the following site and reccommend that all docs rush there for easy compliance (or, if you're lucky, an exemption from this annual nightmare!!!). http://www.osha.cme.edu I thought this was a one-time requirement in 1992, but I was wrong...It's EVERY YEAR!!!! From owner-diagnostics@net.bio.net Wed Sep 11 23:00:00 1996 Path: biosci!biosci!not-for-mail From: lmcgrath@foothill.net Newsgroups: bionet.diagnostics Subject: Gen-Probe Date: 12 Sep 1996 14:18:35 -0700 Organization: Foothill Net Lines: 4 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <519nt9$eg1@ns2.foothill.net> NNTP-Posting-Host: net.bio.net Does anyone know if the Gen-Probe Chlamydia & Gonorrhea combination Test is performing well in asymptomatic cases? Thanks! larel From owner-diagnostics@net.bio.net Wed Sep 11 23:00:00 1996 Path: biosci!biosci!not-for-mail From: mliguori@aol.com (MLIGUORI) Newsgroups: bionet.diagnostics Subject: hollow fibers for hybridoma culture Date: 11 Sep 1996 18:18:49 -0700 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <5158o7$5et@newsbf02.news.aol.com> Reply-To: mliguori@aol.com (MLIGUORI) NNTP-Posting-Host: net.bio.net I am investigating various manufacturers of hollow fiber bioreactors used in the cultivation of mammalian cells. I am compiling a list of as many manufacturers as I can identify. If you know of a manufacturer, please e-mail me at MLIGUORI.aol.com so I can include it on a list. Any evaluation of the performance of the hollow fiber would be welcome as well. From owner-diagnostics@net.bio.net Fri Sep 13 23:00:00 1996 Path: biosci!biosci!not-for-mail From: boismier@umich.edu (Tom Boismier) Newsgroups: bionet.diagnostics Subject: Re: OSHA and Managed Care Plan - SPAM! Date: 14 Sep 1996 04:50:17 -0700 Organization: University of Michigan Lines: 6 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <51biog$9pj@lastactionhero.rs.itd.umich.edu> References: <511r94$sem@news0-alterdial.uu.net> NNTP-Posting-Host: net.bio.net In article <511r94$sem@news0-alterdial.uu.net>, bratliff@jax-inter.net says... They just want you to buy their "info" kits... What's fascinating (and kinda depressing) is that the site has an educational designation... From owner-diagnostics@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "M. Nishibori" Newsgroups: bionet.diagnostics Subject: [Announce] The Internet Survey of Morphological Laboratory Tests-1996A Date: 20 Sep 1996 13:45:23 -0700 Organization: Tokyo Ikashika University Lines: 28 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <51uvp3$sc2@net.bio.net> Reply-To: mn.mlab@med.tmd.ac.jp NNTP-Posting-Host: net.bio.net Sep. 20, 1996 Announcement of the Internet Survey of Morphological Laboratory Tests-1996A Everyone interested in laboratory medicine or clinical pathology are invited to join the survey project for quality assurance of laboratory tests, this time, with no entry charge. This controlled survey includes morphology on urinary examination, hematology and microbiology, and all procedures are done through the internet. Please check the home page at the address shown below. (Both Japanese and English are used there, so neglect queer characters other than English if you do not understand Japanese language) URL http://202.242.169.152/clap/survey.html (select 'the Internet Survey of Morphological Laboratory Tests-1996A' from the survey list shown there) by: The volunteer group for the internet activity of Japanese Association of Clinical Laboratory Physicians ------------------------------------------------------------------------ from: M. NISHIBORI, M.D., Clinical Laboratory Physician (No.269) Tokyo Ikashika University E-mail: mn.mlab@med.tmd.ac.jp From owner-diagnostics@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Tom Ruddy <76653.1553@CompuServe.COM> Newsgroups: bionet.diagnostics Subject: Leptin Date: 20 Sep 1996 14:09:59 -0700 Organization: IMI Diagnatech Inc. Lines: 11 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <51mnt8$lk7$2@mhadg.production.compuserve.com> NNTP-Posting-Host: net.bio.net I am searching for new sources of leptin tests. New technologies preferably. Thanks, Tom Ruddy 76653.1553@compuserve.com -- TOM RUDDY From owner-diagnostics@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Tom Ruddy <76653.1553@CompuServe.COM> Newsgroups: bionet.diagnostics Subject: Albumin stability Date: 20 Sep 1996 14:10:07 -0700 Organization: IMI Diagnatech Inc. Lines: 11 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <51v17f$31m@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know where I can find reports on the stability of human and bovine serum albumin? I am concerned mainly about ambient room temperature storage of 1) pure albumin, 2) 10% solution and 3) freeze dried or lyophylized states. Thank you! Tom Ruddy 76653.1553@compuserve.com -- TOM RUDDY From owner-diagnostics@net.bio.net Thu Sep 19 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Joseph Postman Newsgroups: bionet.diagnostics Subject: Re: diagnostic of plant disease (fwd) Date: 20 Sep 1996 14:09:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone have additional suggestions for sources of plant-virus-ELISA kits? or an email address for Agdia? jp ---------- Forwarded message ---------- Date: Tue, 17 Sep 1996 11:44:05 -0400 (EDT) From: Joseph Postman To: Jose Villanueva Subject: Re: diagnostic of plant disease Jose, The only company that I know of in the United States is: Agdia, Inc. 30380 County Road 6 Elkhart, Indiana 46514 USA phone: 219 264-2014 fax: 219 264-2153 I don't know how to reach them by email, but will make inquiries. Joseph On Mon, 16 Sep 1996, Jose Villanueva wrote: > Hello: > > I'm looking for a distribuitor of ELISA kits in order to detect virus of > strawberry. Could you help me? > > Thank you, > > -- > Jose Villanueva > Av. Roosevelt 715 E-mail: fqregional@unired.net.pe > Alto Selva Alegre Fax : +51 - 54 - 229296 > Arequipa - PERU. > From owner-diagnostics@net.bio.net Mon Sep 23 23:00:00 1996 Path: biosci!biosci!not-for-mail From: lmcgrath@foothill.net Newsgroups: bionet.diagnostics Subject: Product Choices Date: 24 Sep 1996 14:24:38 -0700 Organization: Foothill Net Lines: 5 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <529jim$rcf@net.bio.net> NNTP-Posting-Host: net.bio.net We are looking for feedback about Genzyme vs. R&D Systems' cytokine assays. Which product perform better and why. Any information out there would be greatly appreciated. Thanks! larel From owner-diagnostics@net.bio.net Mon Sep 23 23:00:00 1996 Path: biosci!biosci!not-for-mail From: srldx@po.iijnet.or.jp (H. Sugisaki) Newsgroups: bionet.diagnostics Subject: Diagnostic industry association Date: 24 Sep 1996 14:04:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: biosci-help@net.bio.net Distribution: world Message-ID: <199609240519.OAA00891@sh0.po.iijnet.or.jp> NNTP-Posting-Host: net.bio.net I appreciate it indeed if anybody suggest me a representative in the following organization with whom I can communicate to exchange diagnostic (in vitro) market data. Thank you very much in advance. (1)European Diagnostic Manufactures Association (2)French Diagnostic Industry Association (3)Health Industry Manufactures Association (USA) H. Sugisaki Section Chief Intellectual Property and Licensing SRL, Inc. Tel: (Japan)-426-48-4017 Fax: (Japan)-426-48-4087 E-Mail: srldx@po.iijnet.or.jp URL: http://www.iijnet.or.jp/SRL-CO/ From owner-diagnostics@net.bio.net Tue Sep 24 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell Newsgroups: bionet.diagnostics Subject: Diff Quick Date: 25 Sep 1996 06:33:19 -0700 Organization: Scottish Agricultural Science Agency Lines: 7 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52bcav$stm@net.bio.net> NNTP-Posting-Host: net.bio.net I'm desperate to get a stain called Diff Quick but I cant find anyone who makes/sells it. If anyone knows a supplier (especially one in England, but anywhere will do), I'll be very grateful if you can E.mail me or ring me. Cheers, Jim Clelland sebcjdc@pop-smtp.ich.ucl.ac.uk or 0171 380 7906 From owner-diagnostics@net.bio.net Wed Sep 25 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Mitchell A Blass Newsgroups: bionet.diagnostics Subject: Specialty Labs in CA stinks Date: 26 Sep 1996 01:39:55 -0700 Organization: Emory University Lines: 15 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52dfgr$s25@net.bio.net> NNTP-Posting-Host: net.bio.net Has anyone else had problems with this lab in CA. They have been screwing around with a sample sent for Leptospira IgG and IgM for 5 days now and still can't give me an answer. I keep getting placed on hold with no respect at all from the respresentatives on the pther end of the line. Who else performs Lepto serology??? Anyone else reporting problems with Specialty Labs???? Mitchell A. Blass, M.D. mblass@emory.edu Dr Kevin O'Donnell "I'm happy, I'm happy Diagnostics and Molecular Biology and I'll punch the man that says I'm not" SASA - Ivor Cutler Edinburgh From owner-diagnostics@net.bio.net Thu Sep 26 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Mitchell A Blass Newsgroups: bionet.diagnostics Subject: I was wrong RE: Specialty Labs Date: 27 Sep 1996 01:08:19 -0700 Organization: Emory University Lines: 10 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52g21j$p16@net.bio.net> NNTP-Posting-Host: net.bio.net Regarding my prior message about specialty Labs CA, I was entirely mistaken. This lab was trying to do an outstanding job on a difficult sample for Leptospira, and indeed they offered to repeat the test as a stat at no charge. I however declined their offer. Unfortunatly, I had written my inflamatory ( and in retrospect untruthful) post prior to speaking with Dr. Barka @ Specialty Labs who was singularly helpful. I wish to appologize for insulting Specialty Labs. A lab which is known around the world for its outstanding science and service. Mitchell A. Blass, M.D. From owner-diagnostics@net.bio.net Sun Sep 29 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Pete Kwasowski Newsgroups: bionet.diagnostics Subject: Re: syphilis serology Date: 30 Sep 1996 04:19:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52oack$csv@net.bio.net> NNTP-Posting-Host: net.bio.net At 02:19 30/09/96 -0700, you wrote: >Over the last year we have found a number of sera that were moderately >positive for TPHA, weak positive FTA and negative for reaginic tests. >Although confirmed on retesting, after freezing and thawing the sera became >negative. We are using the kit marketed in England by Launch as the >'reditest'. I have encountered this problem a few times previously but now >it seems much more frequent. > >Has anybody else experienced this, or got any views? > >Thanks. > >Steve Barrett >--------------------------------------------------------------------------- > >Dr. S.P. Barrett, >Microbiology Department, >St. Mary's Hospital >Praed St., >London, >W2 INY >Phone UK 01717251572 >Fax UK 01717251856 > > > > > I've no idea about the specifics of your test, however, loss of titre post freeze/thawing makes me think that you're loosing IgM immunoreactivity in your samples. We have found this with early bleeds after priming immunizations when making polyclonal antisera. Which is one of the reasons we store our antisera at 4 deg C with azide, rather than frozen. Just my "two bob's worth". Pete Dr P Kwasowski ClifMar Associates Limited School Biological Sciences University of Surrey Guildford, GU2 5XH United Kingdom Tel/FAX: +44 (0)1483 259708 E-mail: P.Kwasowski@surrey.ac.uk http://www.surrey.ac.uk/SBS/comps/clifmar.html _\\|//_ (-@-@-) /----------------------------------ooO-(_)-Ooo-------------------------------\ From owner-diagnostics@net.bio.net Sun Sep 29 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Tong Kwok Leung Newsgroups: bionet.diagnostics Subject: Extraction of RNA from stool for RT-PCR Date: 30 Sep 1996 02:19:44 -0700 Organization: Tong Kwok Leung Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52o3bg$23j@net.bio.net> NNTP-Posting-Host: net.bio.net I am working on stools for RT-PCR of Norwalk viruses. I have tried four different methods of RNA extraction : 1. Boom's method 2. CTAB 3. Promega's RNA extraction kit and 4. Spin cartridge from Gibco. However, the results are not satisfactory. I don't know if there are any problems caused due to the extraction methods or because of the quantity of the related virus was too low. Can anyone give me advice on how to solve the above problems? Many thanks! From owner-diagnostics@net.bio.net Sun Sep 29 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Stephen P Barrett [Medicine]" Newsgroups: bionet.diagnostics Subject: Re: syphilis serology Date: 30 Sep 1996 02:19:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52o3ab$22o@net.bio.net> NNTP-Posting-Host: net.bio.net Over the last year we have found a number of sera that were moderately positive for TPHA, weak positive FTA and negative for reaginic tests. Although confirmed on retesting, after freezing and thawing the sera became negative. We are using the kit marketed in England by Launch as the 'reditest'. I have encountered this problem a few times previously but now it seems much more frequent. Has anybody else experienced this, or got any views? Thanks. Steve Barrett --------------------------------------------------------------------------- Dr. S.P. Barrett, Microbiology Department, St. Mary's Hospital Praed St., London, W2 INY Phone UK 01717251572 Fax UK 01717251856 From owner-diagnostics@net.bio.net Sun Sep 29 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Dave Jensen Newsgroups: bionet.diagnostics Subject: AACC Career Meeting Recap, So. Cal. Date: 30 Sep 1996 02:22:30 -0700 Organization: Search Masters International Lines: 120 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52o3gm$26l@net.bio.net> NNTP-Posting-Host: net.bio.net I attended a meeting called "Refining Employment Skills In An Era of Change", presented by the American Association for Clinical Chemistry chapter in the Southern California area. It was attended by about 100 scientists and technical professionals from academia, industry, and clinical environments. "Patterns Of Change" - Presented by Owen Ash, PhD, AACC secretary and VP of ARUP Laboratories in Salt Lake City, Utah. Dr. Ash's company deals with exotic tests that many hospitals and companies are not doing any longer, both in the development of new tests as well as the standard battery of clinical chemistry work. He commented that although his talk was about the rapid change undergoing the healthcare and scientific job markets, his company currently has over 80 open positions. Salt Lake City is a "boom town" now, and this is one reason for the growth. Other points that he made include: - Less positions in clinical chemistry in the future in the hospital environment. Most positions there will be BS/MS level medtech or chemistry jobs, while the PhD and experienced clinical chemist will find more jobs opening up in private service firms: labs that have specialized and thrive on work given them by the big healthcare institutions. - A hot area for the future will be in information systems within the healthcare arena. Those who combine a medical technology background and computer expertise. "Dealing With Stress During Difficult Times" - Presented by Wiley Johnson, PhD. Dr.Johnson presented a fascinating study of the factors which induce stress, and in particular how these develop in and around the career. The job seeker is, obviously, one of the biggest candidates for stress. Here are a couple of points that Wiley made: - It's not change that people resist and which causes stress, it is transition. - The six areas most affected by the transition period: Relationships with others, Personal Identity, Sense of the Future, Sense of Meaning, Loss of Control, Turf. - He reported on his belief that a full spectrum of emotion is healthier for the nervous system than just a steady diet of positive thinking; advises that people push on through their personal transitions with a positive outlook, but when the negative occurs, to recognize it and to experience that emotion as well. "Great Resumes" - Presented by David Markowitz, Markowitz Associates This was primarily a hands-on workshop style session in which we discussed various forms of resumes described by the speaker. We learned how to prioritize the material that needs to be on the resume, and how important the first third of the first page really is. David Markowitz strongly advised having a number of resume or CV formats, made up for different kinds of jobs. "In the old days," he said, "the batch size of a resume was about 200. You'd take it into the printer and have them run a couple hundred copies on their offset printer. Now, with personal computers and Microsoft Word, we have in effect a batch size of 1 resume. Customize, customize, customize." A great comment about age discrimination: "There's no such thing as age discrimination. What you run into is SALARY discrimination. It's just plain cheaper to hire someone with less experience." "Insider's Secrets To The Science Job Market" - Presented by Dave Jensen I gave a talk about the five paths to the job market and how to optimize all the various aspects of the job search. Material from this available at http://www.bio.com/hr/search/search_1.html, and readers of this newsgroup have heard enough from me that I don't need to present it again here. "Effective Networking" - Presented by Susan Linn, Orange County Networking Directory Susan gave a hilarious talk about the importance of networking. Although she had to speak over the din of the luncheon, she was superb. In effect, her presentation taught us all a handful of solid networking ideas. We came away with a new appreciation of how networking is a LIFE skill and not just a JOB SEEKING skill. "Strategies and Resources For a Successful Search" - Presented by Ray Griffin, Sr. VP, Drake Beam and Morin, Inc. A Vice President of the world's largest outplacement agency gave a great talk on the joys of the job search. Ray brought a lot of humor to his talk, as well as a number of excellent recommendations. Here are a few of them: - Read the excellent book "Transitions" by Wm. Bridges, and his favorite called "Emotional Intelligence", by Goleman. - Regarding the use of networking resources: "No one is going to take your career as seriously as you are. Take control over the process." - 20% temporary employment through specialized agencies will go up to 40% by the year 2000. He forecasts new meaning for the word "temp." - The best interview question that he was ever asked by an interviewing candidate (when he was in industry): "What are the problems around here that wake you up in the middle of the night? I'd like to help you solve those." - Your two greatest friends while networking and interviewing: "Persistence and a positive attitude." Hope that this recap was of interest. If you attend a career related seminar, please share your thoughts with me. regards Dave Jensen Managing Director, Search Masters International http://smi.bio.com/ From owner-diagnostics@net.bio.net Mon Sep 30 23:00:00 1996 Path: biosci!biosci!not-for-mail From: "Dr. J.P. Clewley" Newsgroups: bionet.diagnostics Subject: Re: Extraction of RNA from stool for RT-PCR Date: 1 Oct 1996 02:25:48 -0700 Organization: UK HGMP Resource Centre Lines: 19 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <52qo2s$qvt@net.bio.net> NNTP-Posting-Host: net.bio.net Tong Kwok Leung wrote: >I am working on stools for RT-PCR of Norwalk viruses. >I have tried four different methods of RNA extraction : >1. Boom's method >2. CTAB >3. Promega's RNA extraction kit and >4. Spin cartridge from Gibco. >However, the results are not satisfactory. I don't know if there >are any problems caused due to the extraction methods or because of >the quantity of the related virus was too low. >Can anyone give me advice on how to solve the above problems? You could try phenol/chloroform extraction before Boom extraction; make sure the stools are homogenised well before extraction. Jon Clewley