From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Christian Mouton Newsgroups: bionet.diagnostics Subject: PCR-DNA probe assay for Bacteroides forsythus Date: 4 Dec 1996 07:24:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453f$vh@net.bio.net> NNTP-Posting-Host: net.bio.net The following paper will be published in the December 1996 issue of Molecular and Cellular Probes: A PCR-DNA probe assay specific for Bacteroides forsythus Emmanuelle Guillot and Christian Mouton Abstract Bacteroides forsythus is a fastidious anaerobic gram negative organism associated with active periodontal disease. The ability of random amplified polymorphic DNA (RAPD) fingerprinting to generate species-specific markers was exploited towards the construction of a polymerase chain reaction (PCR)-DNA probe assay specific for B. forsythus. The strategy included the four following steps: 1) construction of a first generation DNA probe based on a 507-bp RAPD species-specific marker; 2) cloning and sequencing the 507-bp RAPD marker; 3) design of the primer pair Bf 392-1/Bf 392-2 flanking a 392-bp specific internal sequence; and 4) synthesis of quantities of a 392-bp second generation DNA probe by PCR amplification. The PCR-DNA probe assay includes a PCR amplification of a 392-bp specific sequence in the genomic DNA of B. forsythus strains followed by hybridization with the 392-bp digoxigenin-labelled second generation probe. We observed strong, specific hybridization with the amplified DNAs from 11 strains of B. forsythus and no cross-hybridization with the PCR products from 22 foreign species. The PCR-DNA probe assay must be seen as a highly specific and sensitive method for the detection of B. forsythus in mixed infections. Christian Mouton, DCD, DSO Groupe de Recherche en Ecologie Buccale Faculte de medecine dentaire, Universite Laval Quebec (Quebec) G1K 7P4 CANADA tel. (418)656-5872; fax. (418)656-2861 From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Paul Becquart Newsgroups: bionet.diagnostics Subject: Re: ELISA for Alveolar Echinococcus antigen(s)? Date: 4 Dec 1996 07:24:33 -0800 Organization: GIS INFOBIOGEN, 7 rue Guy Moquet BP8, 94801 VILLEJUIF, France Lines: 19 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453h$vs@net.bio.net> NNTP-Posting-Host: net.bio.net Zafer Altin (zafer@abconcepts.com) wrote: : We are interested to find out where we can purchase ELISA(preferably) : kits for detection of Alveolar Echinococcus antigen(s). : Any help is greatly appreciated. : end : Zafer Altin, Ph.D. : Antibody Concepts : South San Francisco, CA : Tel/Fax: (415) 738 9776 : e-mail: zafer@abconcepts.com : web site:http://www.abconcepts.com In france, you should connect to a civil hospital located in "Besancon" (estern france). This hospital is closed to the endemic region of echinococcus, this is the reason why they are able to answer to you. If you are interested, i'll look for the phone number. PB From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 4 Dec 1996 07:25:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 243 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58454i$13e@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net Kevin O'Donnell "Work as if you were in the early days Leith of a better nation" - Alasdair Gray http://www.compulink.co.uk/~embra/ From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Hong- Gil Nam Newsgroups: bionet.diagnostics Subject: [Q} finding a mutation site on a BAC clone Date: 4 Dec 1996 07:24:55 -0800 Organization: POSTECH Lines: 20 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <584547$12e@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Netters: I am trying to clone a gene for gi gene in Arabidopsis. We have isolated a BAC clone (150kb) that are very likely to encompass the mutation. We would like to find the mutation site in the BAC clone. The BAC clone is form wild type and we have three independent alleles of mutants. I would appreciate if someone give an inforamtion on how to find a mutation site on a BAC clone in our situation? Hong Gil Hong Gil Nam Department of Life Science Pohang University of Science and Technology Hyoja Dong, Pohang Kyungbuk, 79-784 South Korea From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Leahcim Newsgroups: bionet.diagnostics Subject: EURO. TISSUE REPAIR SOC. WEB SITE: Date: 4 Dec 1996 07:24:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <584543$11s@net.bio.net> NNTP-Posting-Host: net.bio.net Visit the European Tissue Repair Society web site for current information on tissue repair and regeneration. The site is free and updated on a regular basis: http://www.leahcim.demon.co.uk/etrs.htm MDL's HomePage: http://www.leahcim.demon.co.uk/index.htm From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Dr Dave Smart <100307.2661@compuserve.com> Newsgroups: bionet.diagnostics Subject: hepatitis latex agglutination Date: 4 Dec 1996 07:24:48 -0800 Organization: Randox Laboratories Ltd. Lines: 7 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <584540$11f@net.bio.net> NNTP-Posting-Host: net.bio.net I am trying to source manufacturers of latex agglutination kits for the detection of hepatitis B in serum. Can anyone give me any leads please? W. FitzGerald Compuserve 100307,2661 From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Vigfrid Ness Newsgroups: bionet.diagnostics Subject: 96-well polyvinyl plates Date: 4 Dec 1996 07:24:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453u$116@net.bio.net> NNTP-Posting-Host: net.bio.net Hi! I'm running a modified version of an ELISA method and for that I need flexible 96-well polyvinyl plates. Up to now I have been using the Costar round-bottom ones with acceptable result. I would, however, prefer to use flat-bottom plates. I have come across such plates from Falcon but they are a little too big for my plate-shaker and almost too big for my ELISA-reader. Does anyone know about other brands of flat-bottom flexible 96-well polyvinyl plates???? Thanks in advance Viffi vigfrid.ness@veths.no From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Larry Winger Newsgroups: bionet.diagnostics Subject: recombinant Human Thyrotropin (TSH) Receptor supplier? Date: 4 Dec 1996 07:24:43 -0800 Organization: Clin. Biochem., University of Newcastle upon Tyne Lines: 18 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453r$10v@net.bio.net> NNTP-Posting-Host: net.bio.net Any help from anyone regarding commercial (or non-commercial!) suppliers of this important receptor for research purposes, would be much appreciated. Please reply to the group as well as to me, if you have any information. We've looked through altavista. Biosupplynet, BioSpace, Linscott's (found it in Immundiagnostik, Germany, which now seems to be defunct), and are almost at wit's end. Many thanks for any help in this regard Larry Winger -- Larry Winger PhD PGCE http://georgia.ncl.ac.uk From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Bryan Kiehl Newsgroups: bionet.diagnostics Subject: HSV Serology Date: 4 Dec 1996 07:24:38 -0800 Organization: GenBio Lines: 7 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453m$10d@net.bio.net> NNTP-Posting-Host: net.bio.net I am planning to make a serology test differentiating herpes simplex type 1 and type 2 antibodies. I plan to use recombinant protein for the HSV-2 antigen. Are there sources for this? Bryan Kiehl GenBio, San Diego b3748@cts.com From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: tigergil@aol.com Newsgroups: bionet.diagnostics Subject: Re: dna fingerprinting Date: 4 Dec 1996 07:24:37 -0800 Organization: AOL http://www.aol.com Lines: 4 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453l$104@net.bio.net> NNTP-Posting-Host: net.bio.net write to: the director, afip, washington d.c. and ask for information from dr.v.weedn md the leader the dna lab will help you....tigergil@aol.com From owner-diagnostics@net.bio.net Tue Dec 03 22:00:00 1996 Path: biosci!biosci!not-for-mail From: "Carl M. Good III" Newsgroups: bionet.diagnostics Subject: Saliva for diagnostic testing Date: 4 Dec 1996 07:24:29 -0800 Organization: UltraNet Communications, Inc. Lines: 4 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58453d$vc@net.bio.net> NNTP-Posting-Host: net.bio.net I would like to correspond with people who are interested in saliva for diagnostic testing. Please contact me at carlg@ultranet.com Thanks ---- Carl From owner-diagnostics@net.bio.net Mon Dec 09 22:00:00 1996 Path: biosci!biosci!not-for-mail From: "Arsen P.Fiks, M.D., Ph.D." Newsgroups: bionet.diagnostics Subject: Help to create "Dictionary of self-experimenters" Date: 10 Dec 1996 11:50:52 -0800 Organization: Ripco Communications Inc. Lines: 45 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58keus$p7g@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleague, I am appealing to everybody who has performed any kind of experiments on him / herself. I am appealing also to those who can recollect any kind of self-experiments performed by their colleagues in the past. There is no question that data and results of all kinds of self-experiments should not disappear from the face of the earth in the stream of history. Every one of those experiments is unique, and the results of them may benefit the humanity in the future. The great example would be experiments of Werner Forssmann, M.D., who in 1919 inserted a catheter into his heart by himself. Many self-experiments have lead to disappearance of a great number of dangerous infectious diseases. I need the following information: 1) short biographical data, 2) the nature and outcome of the experiment, and 3) bibliographical information: experimenter's publications and/or publications about him / herself. I would greatly appreciate the feedback from anybody who is going to respond to my request with any kind of related information. Sincerely, Arsen P.Fiks, M.D.,Ph.D. Cancer Pathology P.S. My goal is to compose a "Dictionary of Self-experimenters". Please respond to: Arsen P.Fiks, M.D.,Ph.D. 2050 Valencia Drive Northbrook, IL 60062 USA tel : (847) 5649189 E-mail: shleym@ripco.com (Please point in subject-"For Arsen P.Fiks") From owner-diagnostics@net.bio.net Tue Dec 10 22:00:00 1996 Path: biosci!biosci!not-for-mail From: wang yu quan Newsgroups: bionet.diagnostics Subject: Micobacterium Tuberculosis Date: 11 Dec 1996 12:02:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58n3vr$q5d@net.bio.net> NNTP-Posting-Host: net.bio.net Does anybody know th source of Micobacterium Tuberculosis antigen? I'll be very appreciated if you can help me to buy this antigen as soon as possible. It's for lab reaserch of my friend in Chinese largest and most famous hospital-- the Union Hospital. Looking forward to your reply. Best Regards, Wang Yuquan From owner-diagnostics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Pete Kwasowski Newsgroups: bionet.diagnostics Subject: Re: 96-well polyvinyl plates Date: 12 Dec 1996 08:41:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58pcjm$3ep@net.bio.net> NNTP-Posting-Host: net.bio.net At 07:24 04/12/96 -0800, you wrote: >Hi! > >I'm running a modified version of an ELISA method and for that I need >flexible 96-well polyvinyl plates. Up to now I have been using the Costar >round-bottom ones with acceptable result. I would, however, prefer to use >flat-bottom plates. I have come across such plates from Falcon but they are >a little too big for my plate-shaker and almost too big for my ELISA-reader. >Does anyone know about other brands of flat-bottom flexible 96-well >polyvinyl plates???? > >Thanks in advance > >Viffi >vigfrid.ness@veths.no > > We have always used Dynatech (now Dynex) plates with good results. But, I'm not too sure how they what their relative size is. Pete Dr P Kwasowski ClifMar Associates Limited School Biological Sciences University of Surrey Guildford, GU2 5XH United Kingdom Tel/FAX: +44 (0)1483 259708 E-mail: P.Kwasowski@surrey.ac.uk http://www.surrey.ac.uk/SBS/comps/clifmar.html _\\|//_ (-@-@-) /----------------------------------ooO-(_)-Ooo-------------------------------\ From owner-diagnostics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!biosci!not-for-mail From: ECHO Strategies Group Newsgroups: bionet.diagnostics Subject: Resource for Clinical Investigators Date: 12 Dec 1996 08:42:45 -0800 Organization: MindSpring Enterprises Lines: 32 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58pcm5$3gf@net.bio.net> NNTP-Posting-Host: net.bio.net Physicians and investigators: As you know, half the battle of participation in clinical trials is getting your program in front of companies as they release new research dollars. To view the latest news in who is doing clinical trials, visit Avicenna Online Medical Information at: www.avicenna.com and visit the Clinical Trials section. You can enter your research profile into the SmartSearch (TM) investigator database. This section also has links to additional Clinical Trial Resources including: AIDS Clinical Trials Information Service (ACTIS) Brain Tumor Clinical Trials CenterWatch , and Sterling Clinical Resources. (Please note: these sites are for RESEARCHERS ONLY, not for individuals seeking inclusion as subjects/participants) While you are at the site, be sure to visit the rest of the site and take advantage of their other free services such as the Clinical Outlines, Free Medline (not as busy as MedScape(TM)), and the MediQual(TM) Outcomes Database. Kevin O'Donnell "Work as if you were in the early days Leith of a better nation" - Alasdair Gray http://www.compulink.co.uk/~embra/ From owner-diagnostics@net.bio.net Wed Dec 11 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Suzy Newsgroups: bionet.diagnostics Subject: FragX E/Point mutation on X? Date: 12 Dec 1996 08:41:22 -0800 Organization: HookUp Communication Corporation, Vancouver, BC, CANADA Lines: 14 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <58pcji$3ei@net.bio.net> NNTP-Posting-Host: net.bio.net Hi. What are the specific tests for fragile x E and point mutation of the X gene? My son had the fragX southern blot(apprently the regular FragX A test). Since then I've been told even though result were normal because he behaviourally and physically meets the majority of criteria that he probably has frag X but FragX E or a point mutation. Suzy (03/67) HFA/AS. Just another alien on earth with my own little colony. Stephen my sunshine (01/92) ADHD/PDD, and Alyssa my aly cat (03/90) ODD. Suzy Suzy'a Home Page: Java/forms/Graphics version: http://www.Suzy.home.ml.org From owner-diagnostics@net.bio.net Sat Dec 14 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Joseph Postman Newsgroups: bionet.diagnostics Subject: Virus Diseases of Temperate Fruits - Symposium (fwd) Date: 15 Dec 1996 03:15:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <590mkh$lu6@net.bio.net> NNTP-Posting-Host: net.bio.net The 17th International Symposium on Virus and Virus-like Diseases of Temperate Fruit Crops Will be held in: Bethesda, Maryland, USA June 23-27, 1997 This symposium is organized by the International Society for Horticultural Science - Working Groups on Virus Diseases of Fruit Trees and Virus Diseases of Small Fruits, and by the US Department of Agriculture, Agricultural Research Service. Sessions will be held at the Natcher Conference Center on the campus of the National Institutes of Health, Bethesda, Maryland, about 8 miles from downtown Washington, D.C. To receive the second circular and registration information, please contact: Dr. Ahmed Hadidi USDA/ARS National Germplasm Resources Lab Building 011A, Room 106, BARC-West Beltsville, MD 20705 USA phone: 301-504-6460 fax: 301-504-5551 email: ahadidi@ars-grin.gov From owner-diagnostics@net.bio.net Sat Dec 14 22:00:00 1996 Path: biosci!biosci!not-for-mail From: CRivnak@aol.com Newsgroups: bionet.diagnostics Subject: Re: Micobacterium Tuberculosis Date: 15 Dec 1996 03:15:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <590mkb$ltb@net.bio.net> NNTP-Posting-Host: net.bio.net Greetings! You might check with the Institute for BioTechnology Information (IBI) in Research Triangle Park NC USA. They are rather good at keeping track of such information. Best of Success! Chris Hrivnak An Associate of Sommerset Capital Corporation From owner-diagnostics@net.bio.net Sat Dec 14 22:00:00 1996 Path: biosci!biosci!not-for-mail From: "Oleg S. Vasilyev" Newsgroups: bionet.diagnostics Subject: mathematical simulation in immunology Date: 15 Dec 1996 03:15:23 -0800 Organization: RopNet Lines: 17 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <590mkb$lt6@net.bio.net> NNTP-Posting-Host: net.bio.net Dear sirs! I am occupied with preparation of the review on mathematical models in immunology for Russian scientific press. I shall be sincerely glad to include in this review the information on your researches in the field of mathematical simulation in immunology. Please, answer. Yours faithfully, Oleg S. Vasilyev. NATIONAL RESERCH CENTR-INSTITUTE OF IMMUNOLIOGY RUSSIA, MOSCOW mathmed@ropnet.ru From owner-diagnostics@net.bio.net Sun Dec 15 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Doctorkev Newsgroups: bionet.diagnostics Subject: Lacto bacteria Date: 16 Dec 1996 08:26:07 -0800 Organization: AOL, http://www.aol.co.uk Lines: 7 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <593t6v$g6b@net.bio.net> NNTP-Posting-Host: net.bio.net I would be very interested if anybody could tell me of a source for an antiubody to lactobacteria, and also of a source of the bacteria itself. Thanks in advance Kevin Jones From owner-diagnostics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Mark Peters Newsgroups: bionet.diagnostics Subject: b6.D CYCLOSPORIN REMISSION, early onset Date: 17 Dec 1996 10:56:00 -0800 Organization: GENIUS QUEST Lines: 12 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <596qc0$cv9@net.bio.net> NNTP-Posting-Host: net.bio.net b6 Cyclosporin causes remission from early onset diabetes Diabetes (E8X), 1994 Nov; 43 (11): 1291-6 "We have investigated whether glutamic acid decarboxylase (GAD) autoantibodies (GAD65 Ab) were affected by cyclosporin therapy and were related to subsequent non-insulin-requiring remission and loss of glucagon-stimulated c-peptide response in 132 recent-onset insulin-dependent diabetes mellitus (IDDM) patients treated with cyclosporin or placebo for 12 months. " From owner-diagnostics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Mark Peters Newsgroups: bionet.diagnostics Subject: b7.D CsA '-,_T cell independent prod. IL1 Date: 17 Dec 1996 10:56:04 -0800 Organization: GENIUS QUEST Lines: 13 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <596qc4$cvc@net.bio.net> NNTP-Posting-Host: net.bio.net b7 Cyclosporin inhibits T cell-independent production if IL1 Methods Find Exp Clinh Pharmacol (LZN), 1986 Fep; 8 (2):85-9 The effects of CsA (1 ng/ml-1 microgram/ml) on T cell- independent production of IL1 by irradiated, carrageenan-stimulated peritoneal macrophages were also examined. Up to 50 ng/ml CsA had no effect on IL1 production but higher concentratins produced a dose-dependent inhibition that was complete at 1 microgram.ml. These results show that CsA inhibits both T cell-independent production if IL1 and IL1 dependent expression oof reactivity to IL2. From owner-diagnostics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Mark Peters Newsgroups: bionet.diagnostics Subject: b8.D _,-^MONOCYTES @ C.a, newly diagnosed Date: 17 Dec 1996 10:56:04 -0800 Organization: GENIUS QUEST Lines: 31 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <596qc4$cve@net.bio.net> NNTP-Posting-Host: net.bio.net [KH-CEI.94] K. Josefsen, H. Nielsen, S. Lorentzen, P. Damsbo & K. Buschard Circulating monocytes are activated in newly diagnosed type 1 diabetes mellitus patients Clin Exp Immunol 1994; 98:489-493 "Investigations in the BB ret and the non-obese diabetic (NOD) mouse have provided substantial evidence for the involvement of the monocyte/macrophage system in the development of type 1 diabetes mellitus. However, it is not known whether monocytes play the same role in the pathogenesis of human type 1 diabetes. We investigated this problem in a longitudinal study of 29 recent-onset type 1 diabetes mellitus patients, Monocyte chemotaxis, phagocytosis and superoxide production as well as metabolic and hematological parameters were studied immediately after diagnosis and 6 months later. At diagnosis the patients had activated casein and C5a chemotaxis(casein 70+-9 versus 150 +-5 (mean+- s.e.m.), P<0.001; C5a 137+-10 versus 158+-5, P<0.05 (activation immobilizes monocytes, reducing the measured values)), and activated superoxide production(3.6+-0.3 versus 3.0+-0.3, P<0.05). After 6 mo.casein chemotaxis (115+-16 versus 150+-5, P<0.06 and CANDIDA PHAGOCYTOSIS 3.3+-0.1 versus 2.8+-0.2,P<0.001) were still activated. There was no correlation with other clinical or paraclinical parameters. We conclude that the circulating monocytes in newly diagnosed type 1 diabetes patients are activated. It is reasonable to expect that monocytes at the local site of inflammation in pancreas are even further activated. This could play a pathogenic role in B cell destruction. From owner-diagnostics@net.bio.net Mon Dec 16 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Mark Peters Newsgroups: bionet.diagnostics Subject: b6.D CYCLOSPORIN REMISSION, early onset Date: 17 Dec 1996 10:56:13 -0800 Organization: GENIUS QUEST Lines: 12 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <596qcd$cvt@net.bio.net> NNTP-Posting-Host: net.bio.net b6 Cyclosporin causes remission from early onset diabetes Diabetes (E8X), 1994 Nov; 43 (11): 1291-6 "We have investigated whether glutamic acid decarboxylase (GAD) autoantibodies (GAD65 Ab) were affected by cyclosporin therapy and were related to subsequent non-insulin-requiring remission and loss of glucagon-stimulated c-peptide response in 132 recent-onset insulin-dependent diabetes mellitus (IDDM) patients treated with cyclosporin or placebo for 12 months. " From owner-diagnostics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Holger Scheibner Newsgroups: bionet.diagnostics Subject: luminescent oxygen channeling immunoassay Date: 19 Dec 1996 08:41:23 -0800 Organization: Freie Universitaet Berlin Lines: 8 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <59br7j$r6o@net.bio.net> NNTP-Posting-Host: net.bio.net I read about a new method called luminescent oxygen channeling immunoassay. It is made by Behring Diagnostics. I would like to have more details. Any information is very appreciated. Thanks in advance H. Scheibner From owner-diagnostics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Simon Eaton Newsgroups: bionet.diagnostics Subject: SSIEM (Society for the Study of Inborn Errors of Metabolism) Symposium Date: 19 Dec 1996 08:45:38 -0800 Organization: Newcastle University, UK Lines: 16 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <59brfi$rk6@net.bio.net> NNTP-Posting-Host: net.bio.net The First Announcement of the SSIEM (Society for the Study of Inborn Errors of Metabolism) Annual Symposium, tobe held in Gothenberg, Sweden, in September 1997 is online at the SSIEM website: http://www.ncl.ac.uk/~nchwww/SSIEM/ssframe.html -- __________________________________________________________ Simon Eaton,Research Associate,E-mail S.J.Eaton@ncl.ac.uk Mail: Sir James Spence Institute of Child Health Royal Victoria Infirmary,Newcastle upon Tyne NE1 4LP United Kingdom FAX:+44 191 202 3041, Phone+44 191 202 3043 http://www.ncl.ac.uk/~nchwww/metab.html __________________________________________________________ From owner-diagnostics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Pekka Makinen Newsgroups: bionet.diagnostics Subject: Re: luminescent oxygen channeling immunoassay Date: 19 Dec 1996 11:21:16 -0800 Organization: Runciter Associates Lines: 15 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <59c4jc$b6f@net.bio.net> NNTP-Posting-Host: net.bio.net anthrax@zedat.fu-berlin.de (Holger Scheibner ) writes: >I read about a new method called luminescent oxygen channeling immunoassay. >It is made by Behring Diagnostics. I would like to have more details. Any >information is very appreciated. Look at Clinical Chemistry, vol. 42 p. 1518-1526 (1996) which has an article named "Luminescent oxygen channeling immunoassay (LOCI TM): sensitive, broadly applicable homogeneous immunoassay method" by Ullman, F.E. et al. -- ____________________________ Nil sub sole stabile http://www.iki.fi/~pemakine/ Fiat lux From owner-diagnostics@net.bio.net Wed Dec 18 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Pokinatcha Stu Newsgroups: bionet.diagnostics Subject: Purification of ASO from serum. Date: 19 Dec 1996 08:45:34 -0800 Organization: J. Fleishman Biotechnology Lines: 18 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <59brfe$rjo@net.bio.net> NNTP-Posting-Host: net.bio.net I'm looking for some help in the purification of Anti-Streptolysin O antibodies from serum. I've tried to sensitize SLO antigen onto various inert supports from immunoaffinity particles to hollow fibers. My best recovery to date has been only about 15 - 20 % total antibodied applied. In addition, I've only been able to reuse the column about 6 times. Any help would be greatly appreciated. I will pay for the successful procedure. For more information contact Jeff Fleishman in the USA at Simplicity Diagnostics, 1-800-445-5487 or 1-215-441-8279. FAX 1-215-672-7149. or e-mail jfleishman@p3.net From owner-diagnostics@net.bio.net Fri Dec 20 22:00:00 1996 Path: biosci!biosci!not-for-mail From: Bryan Kiehl Newsgroups: bionet.diagnostics Subject: Mononucleosis Samples Date: 21 Dec 1996 15:02:55 -0800 Organization: GenBio Lines: 27 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <59hqav$eus@net.bio.net> NNTP-Posting-Host: net.bio.net We are beginning a field trial testing dipsticks that detects heterophile, EBV VCA IgM and IgG, EBNA IgG, CMV IgG and IgM, and toxoplasma IgG. In addition to the routine samples, we wish to test the following: EBV infections that are heterophile negative (presumably from pediatric patients) Samples from patients documented to have CMV infections and symptoms consistent with mononucleosis (e.g., WBC differential right shift) Samples from patients documented to have toxomplasosis and symptoms consistent with mononucleosis Our study is beginning in two U.S. sites, but we plan to expand into at least one additional site. If interested, please let me know. I can be contacted by email, FAX (619-592-9400) or phone (619-592-9300 x309). Bryan Kiehl GenBio, San Diego b3748@cts.com 619 592-9300 ext 309 619 592-9400 (FAX) From owner-diagnostics@net.bio.net Sun Dec 29 22:00:00 1996 Path: biosci!biosci!not-for-mail From: tjiffar@marlin.utmb.edu Newsgroups: bionet.diagnostics Subject: Transformation of human T-cells with Herpesvirus saimiri Date: 30 Dec 1996 11:42:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: kevin@embra.compulink.co.uk Distribution: world Message-ID: <5a95va$fik@net.bio.net> NNTP-Posting-Host: net.bio.net I am interested in transforming CD8+ T cell clones with Herpesvirus saimiri, and I was wondering if anyone who has had success (or failure) with this could give me advice about aspects of the project which might not be found in the current literature. I am trying to find out about aspects of protocols which are not explicitly detailed in manuscripts, but which might be critical for success. Also, how difficult is it to successfully carry out this procedure?