From owner-diagnostics@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Greg Forbes Newsgroups: bionet.diagnostics Subject: PAGE problem Date: 7 May 1997 01:58:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5kpg8i$37c@net.bio.net> NNTP-Posting-Host: net.bio.net GPI is a useful marker for plant pathogenic fungus Phytophthora infestans. Some 7 alleles have been identified, all of which are known by their migration distances relative to a standard allele which was given an arbitrary value of 100. Most alleles can be easily identified on cellulose acetate and Tris-Glycine buffer, and/or on starch gels with TC6 tank buffer and His 6 gel buffer. Unfortunately, the 90 allele can not be easily distinguished from the 100 allele using these systems. Following advice from a collegue, we've been using 12 or 14 % acrylamide gels, with Tris-Borate pH 9.0, Tris-Glycine pH 8.5 or 8.8 as tank buffers, and Tris-HCl pH 8.9 as gel buffer, with or without a stacking gel, Tris-HCl pH 6.9. All this has been done on 1mm thick gels in a vertical system. The enzyme migrates really slowly, only about 2cm when the bromophenol blue has run off the end of the gel (16cm) and the only alleles to separate well are 100/122, which separate much better in starch. There`s no streaking and the bands are quite neat, we I don`t think there`s anything wrong with the extracts. We're about to try reducing the % of acrylamide, but if anyone can help resolve this problem with some other usaful suggestions, we'd be very grateful. Greg Forbes Lynn Erselius Greg Forbes International Potato Center (CIP) P.O. 17-21-1977, Quito, Ecuador Tel. +593-2-690362; Fax +593-2-692604 From owner-diagnostics@net.bio.net Tue May 06 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 7 May 1997 02:05:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5kpgk8$3jp@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Christian Mouton Newsgroups: bionet.diagnostics Subject: PCR-DNA Probe Assays for Prevotella intermedia and Prevotella nigrescens Date: 9 May 1997 06:59:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5kvakc$m21@net.bio.net> NNTP-Posting-Host: net.bio.net Here is the abstract of a paper to be published in Journal of Clinical Microbiology: PCR-DNA Probe Assays for Identification and Detection of Prevotella intermedia sensu stricto and Prevotella nigrescens Emmanuelle Guillot and christian Mouton The purpose of this study was to construct PCR-DNA probe assays specific for Prevotella intermedia sensu stricto and Prevotella nigrescens based on the ability of random amplified polymorphic DNA (RAPD) fingerprinting to generate species-specific markers. The strategy included four steps: (i) construction of first generation DNA probes from a 850-bp RAPD marker for P. intermedia sensu stricto and a 1,300-bp RAPD marker for P. nigrescens, (ii) cloning and sequencing of each RAPD marker, (iii) design of primer pairs flanking specific internal sequences of 754 bp for P. intermedia sensu stricto and ca. 1,100 bp for P. nigrescens, and (iv) synthesis (by PCR amplification) and digoxigenin labeling of quantities of DNA probes 754 and ca. 1,100 bp in size. The PCR-DNA probe assays combine either PCR amplification of a 754-bp specific sequence in the genomic DNA of strains of P. intermedia sensu stricto and hybridization with the 754-bp digoxigenin-labeled probe or amplification of a ca. 1,100-bp sequence of P. nigrescens and hybridization with the ca. 1,100-bp probe. Specific hybridization was observed with the amplified DNAs from 25 strains of P. intermedia and 24 strains of P. nigrescens, and no reaction with the PCR products from 20 foreign species. The PCR-DNA probe assays described here should allow a highly specific and sensitive detection of P. intermedia sensu stricto and P. nigrescens in mixed infections. Christian Mouton, DCD, DSO Groupe de Recherche en Ecologie Buccale Faculte de medecine dentaire, Universite Laval Quebec (Quebec) G1K 7P4 CANADA tel. (418)656-5872; fax. (418)656-2861 From owner-diagnostics@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Doctorkev Newsgroups: bionet.diagnostics Subject: Protein binding in membranes Date: 12 May 1997 01:37:42 -0700 Organization: AOL, http://www.aol.co.uk Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5l6ksm$5q7@net.bio.net> NNTP-Posting-Host: net.bio.net Anybody out there know where I can get some references on the binding of proteins in membranes? I am trying to understand the nature of the interaction of proteins with membranes (most notably nitrocellulose membranes), but I need some help! All suggestions greatfully received. Thanks Kevin Jones From owner-diagnostics@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Dave Jensen Newsgroups: bionet.diagnostics Subject: Bio-career Discussion Forum Date: 12 May 1997 01:42:14 -0700 Organization: Search Masters International Lines: 44 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5l6l56$65a@net.bio.net> NNTP-Posting-Host: net.bio.net A new, non-commercial career discussion forum is available for your browsing and posting interests. No commercial posts or spam are allowed on this news forum, and it is entirely moderated so as to make it 100% worthy of your contribution. There are hundreds of posts on dozens of topics of interest to anyone in the sciences. If you'd like, post your questions and find dozens of other people who may have had similar experiences and who you can network with or get some advice from. Already, networking subgroups have sprouted from the biotechnology section of this career discussion forum. Here are some topics JUST FROM THE LAST WEEK, and we have hundreds of archived posts: Career change to CRA Should you mention your advisor as a reference ? Best MBA for biotech Liberal Arts Positions? Scientist salaries? Temporary Placement Firms, any good? Multiple job offers, maybe? Salary trends Keeping the interview alive... One worried soon to be graduate. Changing jobs/employers Join our career discussion forum at http://cns.bio.com/hr/forum/ regards, Dave Jensen, Moderator From owner-diagnostics@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!biosci!not-for-mail From: carl good Newsgroups: bionet.diagnostics Subject: Software for scanning and quantitating western blots and immunochromatographic tests Date: 12 May 1997 01:37:10 -0700 Organization: itp Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5l6krm$5pc@net.bio.net> NNTP-Posting-Host: net.bio.net I need software to quantitate a computer image of a western blot or immunchromatographic device. I have heard that NIH has some "freeware" for creating a scan of image density vs position i.e. a chromatogram. This allows one to get quantitative information from gel eletrophoresis, western blots or any pattern of image density once it is scanned into a computer image. Please contact me if you know how to get such software from NIH or a commercial source. Thanks for your help Carl Good From owner-diagnostics@net.bio.net Tue May 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Huub Schellekens Newsgroups: bionet.diagnostics Subject: PCR meeting Date: 14 May 1997 01:17:32 -0700 Organization: XS4ALL, networking for the masses Lines: 31 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5lbses$lut@net.bio.net> NNTP-Posting-Host: net.bio.net Second European Meeting on diagnostic PCR Kurhaus Hotel, The Hague, The Netherlands October 16-17, 1997 A meeting devoted to the practical aspects of diagnostic PCR. Topics: - PCR and the detection of unidentified microorganisms - Clinical relevance of PCR results - PCR and tropical infections - The format of the ideal PCR test - PCR in forensic science - PCR in clinical pathology There will be reviews by leading experts, papers and posters with original research. Invited speakers include: C. J. Cornelisse, Leiden H. Rinder, Munich F. Bonino, Turin R. DeCorte, Louvain B. Niesters, Rotterdam Abstract deadline: June 15, 1997 Information: E-mail: 101745.647@compuserve.com fax: + 31 765221931 Wens Travel From owner-diagnostics@net.bio.net Wed May 14 23:00:00 1997 Path: biosci!biosci!not-for-mail From: AT Newsgroups: bionet.diagnostics Subject: Helicobacter pylori ITALIAN site Date: 15 May 1997 03:15:03 -0700 Organization: ITnet Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5lenn7$h17@net.bio.net> NNTP-Posting-Host: net.bio.net For italian people: the most complete site on Helicobacter pylori is at: VI SFIDO A TROVARE UN SITO CON MAGGIORI INFORMAZIONI ------------------------------------------- dr Alberto Torelli (1997) tel/sgr/fax 019-506054 Alberto.Torelli@pn.itnet.it ------------------------------------------- From owner-diagnostics@net.bio.net Wed May 21 23:00:00 1997 Path: biosci!biosci!not-for-mail From: David Kristofferson Newsgroups: bionet.diagnostics Subject: IMPORTANT - BIOSCI moving to Stanford! Date: 22 May 1997 01:36:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 55 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5m10j1$65r@net.bio.net> NNTP-Posting-Host: net.bio.net After more than a decade of serving the biology community on the BIOSCI project, I have decided that it is time for me to pass the torch. The demands of my career and family are making it increasingly difficult for me to run BIOSCI adequately on a part-time basis, and I've decided that I would do the project more harm than good by clinging to it. Therefore I have concluded an agreement with the Stanford University Libraries to take over the management of the BIOSCI project effective 6 June 1997. Many of you know the work of the Stanford Libraries through HighWire Press which has done an excellent job bringing the Journal of Biological Chemistry, Science Magazine, and other prestigious journals to the WWW (see http://highwire.stanford.edu/). I have every confidence that Stanford will provide an environment where the BIOSCI project can flourish. The BIOSCI team at Daresbury will continue to support the project in Europe, and Dave Mack and Julie Lawrence on the current BIOSCI staff will continue to assist the new team at Stanford. The project will be managed by Serge Taylor of the Stanford Libraries who is responsible for developing Web-based "knowledge environments" for scientists. I will still participate with the project in an advisory capacity but have to phase myself out of day-to-day operations. Many of you who read bionet.announce, bionet.biology.computational, and bionet.jobs.offered (the groups that I moderate) have been aware of the recent posting delays that my busy schedule has inflicted on those newsgroups. I am pleased to announce that Todd McGee, Scientific Advisor of the HighWire Press, will be taking over moderation of those groups from me this week. ** NOTE ** - The BIOSCI hardware will be down for backups on 6 June and will be moved over the weekend of June 7 and 8. We will be changing the IP number of the machine, but the host name and all mailing addresses will remain unchanged. There may be a few days of service disruption during this time due to the move unfortunately, just as we experienced in BIOSCI's previous move. We hope to keep this to a minimum. We will post status reports on the move to bionet.announce before and after it happens. Please watch that newsgroup for details. It has been a pleasure serving all of you since the "early days" of biology on the Internet. I've had the pleasure of not only "knowing" many of you over the Internet, but have also met literally thousands of biologists around the U.S. and elsewhere during the many Internet training seminars that I gave over the last decade. I've been truly fortunate to have been given the chance to play a role in the growth of biology on the Internet. This is something I will always treasure. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-diagnostics@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: adrchen@netvision.net.il Newsgroups: bionet.diagnostics Subject: Computers in cardiology Date: 29 May 1997 01:14:16 -0700 Organization: NetVision LTD. Lines: 4 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5mjdso$r5g@net.bio.net> NNTP-Posting-Host: net.bio.net Experienced cardiologist, computer literate, interested in computers in cardiology educational/research project From owner-diagnostics@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Neurohunt Newsgroups: bionet.diagnostics Subject: Histology expert needed Date: 29 May 1997 01:05:48 -0700 Organization: AOL http://www.aol.com Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5mjdcs$qbd@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a promotable BS or MS level scientist with experience in histology and animal handling and or experience in animal surgery or be willing to be trained in animal surgery. We are a leading biotech firm with research facilities in New York and can provide excellent benefits (health insurance, life insurance, vision plan, 401K, stock options, paid vacations and more). Compensation $25,000 to $50,000 based on experience. A high impact, high profile position with excellent opportunity for advancement. Please contact Scott Shanes by phone at 609-584-8733 Ext. 218, fax resume to 609-584-9575 or E-mail to Neurohunt@aol.com. From owner-diagnostics@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!biosci!not-for-mail From: "Justin K. Moran" Newsgroups: bionet.diagnostics Subject: Source for polyclonal antibodies Date: 30 May 1997 06:32:48 -0700 Organization: Albert Einstein College of Medicine Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <5mmku0$s6a@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I am looking for a company the produces rabbit polyclonal antibodies. If anyone has the name of a company that they have used and were happy with please e-mail me. Thanks for your help. Justin Moran