From owner-diagnostics@net.bio.net Sun Mar 01 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Damian Bond Newsgroups: bionet.diagnostics Subject: ELISAs for Meat speciation Date: 2 Mar 1998 01:48:36 -0800 Organization: Virgin Net Usenet Service Lines: 4 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ddv9k$e3b@net.bio.net> NNTP-Posting-Host: net.bio.net Can anyone recommend commercial ELISAs or source of reagents for research ELISAs for speciation of raw meats? From owner-diagnostics@net.bio.net Tue Mar 03 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 4 Mar 1998 01:56:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6dj8gh$dun@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Tue Mar 03 22:00:00 1998 Path: biosci!biosci!not-for-mail From: changrong liu Newsgroups: bionet.diagnostics Subject: Recruiting Papers for First International Disability Medici= Date: 4 Mar 1998 07:12:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 102 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6djr1o$k4d@net.bio.net> NNTP-Posting-Host: net.bio.net Recruiting Papers for First International Disability Medicine Conference The disability medicine is a newly emerging subject. It includes the prevention, research, diagnosis, treatment, rehabitation, and nursing of all diseases causing by limbers and organs defects and severe malfunction of limbers, organs and psychology, which result from various factors of trauma and diseases. The wide contents of speciality include: preventive medicine, clinical medicine and rehabitation medicine. The disability medicine has just begun. In order to develop the academic exchange of the disability medicine, improve the research level and rehabitative level of the disability medicine, advance the development of the disability medicine, so that we can serve the patients better, serve the society better, we decided to hold "First International Disability Medicine Conference" in the capital of China -Beijing on August 8th, 1998. The meeting was co-organized by "The Chinese Rehabitation Association for disability medicine" and " The house of Journal of the disability medicine". 1. We recruit papers about: a. Disease Prevention b. The basic disease research c. Diagnosis, treatment, rehabitation and nursing of disease d. The research and application of apparatus and artificial limbers 2. People who can attend the meeting include: a. international administrative and technical staffs of disability medicine. b. The leader of Chinese hygiene division, the leaders of Chinese Rehabitation Association, the leaders, administrative staffs and technical staffs working on the rehabitation of disabled patients in the national and endemic disability association, red cross association and political administrative office. c. The technical staffs working on the prevention, research, medical treatment and nursing of diseases (all clinical operative specialities: osteology, brain surgery, cardiothoracic surgery, urinary surgery, orthopedics of burn, general surgery, pediatrics surgery, ophthalmology, otorhinol aryngology, stomatology, gynecology, tumorigenesis, anesthetics, etc. Non-operative specialities: internal medicine, neurology, psychiatry, etc.) , working on medical techniques (All specialities of diagnosis), physical rehabitative therapy (physical therapy, body therapy, acupuncture, massotherapy). d. The leaders and technical staffs working on the treatment of disability, rehabitative apparatus, research, exploitation and application of artificial limb and artificial prosthesis. 3. Guide lines for papers: a. The paper should be about 3,000 words, together with an abstract less than 500 words. (If possible, the paper and the abstract should be submitted with their corresponding English translation ) written on. paper. b. The accepted papers and abstracts of the meeting will be exchanged as meeting materials at the meeting, the authors will be informed the material fee by mail after the acceptance of the papers. 4. How to attend the meeting: People can apply since we inform the meeting. People who want to attend the meeting can mail their papers, the registration cards and fees for reviewing papers (=A3=A430 per paper, papers without the fee will not be reviewed) to Dr. Changrong Liu -- Header of the Hei longjiang paralysis institute in Harbin Medical University (Zip:150086). "papers for meeting" should be marked on the down left side of the envelope. See the definite time and place of the meeting on the formal information. The Chinese rehabitation association for Disability Medicine The house of Journal of disability medicine December 8th, 1997 Guidelines for Abstracts and papers The entire abstract should be about 500 words and paper should be typed on a typewriter in black ink about 3000 words, must be submitted in English. Papers will be received in May 31, 1998 (for acceptance in June 1998). Send the original and two copies of the typed paper together with completed submission form to : International Disability Medicine Conference Dr. Changrong Liu 157 Bao jian Street Harbin Medical University Disability Medicine Editorial Staff Habin, 150086 P. R. China Tel: +86-451-6669485 Fax: +86-451-6669470 E-mail: liucr@ems.hrbmu.edu.cn Registration International Disability Medicine Conference August 8-12, 1998 Please print or type Clearly First name: Middle: Last name: Tittle: Employer: Affiliation: Business Address: City: Country: Zip code: Tel: Fax: E-mail: MAIL TO : International Disability Medicine conference Dr. Changrong Liu 157 Bao Jian Street Harbin Medical University Disability Medicine Editorial Staff Harbin 150086 P. R. China From owner-diagnostics@net.bio.net Wed Mar 04 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Paul Eggleton Newsgroups: bionet.diagnostics Subject: Re: Calreticulin monoclonals Date: 5 Mar 1998 01:33:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6dlrhm$4ls@net.bio.net> NNTP-Posting-Host: net.bio.net Dear All, Does anyone know of, or have a monoclonal antibody against correctly folded human calreticulin (i.e an antibody which can tell the difference between correctly folded and incorrectly folded protein). If you do please contact Steve Johnson on email: johnson@bioch.ox.ac.uk here in our lab in Oxford. Many thanks Paul Eggleton. From owner-diagnostics@net.bio.net Sun Mar 08 22:00:00 1998 Path: biosci!biosci!not-for-mail From: kulik Newsgroups: bionet.diagnostics Subject: external quality assessment Date: 9 Mar 1998 06:09:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e0t7h$f0m@net.bio.net> NNTP-Posting-Host: net.bio.net Hi! I'm interested in external quality assessment programs/organisations for Microbiology. I already know of UK NEQAS and INSTAND in Germany. Does anyone know of others in Europe? Thanks Eva email to: kulik@ubaclu.unibas.ch From owner-diagnostics@net.bio.net Sun Mar 08 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Your Name Newsgroups: bionet.diagnostics Subject: nucleic acid hybridization question Date: 9 Mar 1998 02:09:59 -0800 Organization: Glaxo Wellcome Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e0f5n$ib1@net.bio.net> NNTP-Posting-Host: net.bio.net I am trying to understand the basis of nucleic acid hybridization on solid supports. Is anyone aware of any reference(s) that deal with this, specially in respect to the kinetics of hybridization, effects of probe excess or target excess, etc. etc. Any help will be appreciated. Thanks in advance Sujoy From owner-diagnostics@net.bio.net Mon Mar 09 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Damian Bond Newsgroups: bionet.diagnostics Subject: Re: nucleic acid hybridization question Date: 10 Mar 1998 01:44:22 -0800 Organization: Virgin Net Usenet Service Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e321m$bv1@net.bio.net> NNTP-Posting-Host: net.bio.net Look up the Tepnel Life Sciences web site - they have developed the DARAS instrument for this purpose Your Name wrote in message <6e0f5n$ib1@net.bio.net>... >I am trying to understand the basis of nucleic acid hybridization on solid >supports. Is anyone aware of any reference(s) that deal with this, >specially in respect to the kinetics of hybridization, effects of probe >excess or target excess, etc. etc. Any help will be appreciated. Thanks in >advance > >Sujoy > From owner-diagnostics@net.bio.net Mon Mar 09 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Dre Raffaele Pisanti Newsgroups: bionet.diagnostics Subject: help.Free T3 Date: 10 Mar 1998 07:44:20 -0800 Organization: PARAMEDICAL S.r.l. Lines: 7 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e3n4k$rs5@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for an ELISA test for Free T3.There is someone can help me? Raffaele paramed@general.it From owner-diagnostics@net.bio.net Mon Mar 09 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Damian Bond Newsgroups: bionet.diagnostics Subject: Antibodies against Listeria monocytogenes Date: 10 Mar 1998 01:44:20 -0800 Organization: Virgin Net Usenet Service Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e321k$bur@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for commercially available antibodies - does anyone know a source? Damian Bond From owner-diagnostics@net.bio.net Tue Mar 10 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: custom peptide, oligos Date: 11 Mar 1998 08:55:09 -0800 Organization: BT Internet Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e6fld$63s@net.bio.net> NNTP-Posting-Host: net.bio.net I have located a directory of life science products, reagents and services listing the above at: http://www.corniche.com This appears to be a useful searchable resource which is free to use. Jeff Slater From owner-diagnostics@net.bio.net Tue Mar 10 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Spangenberg Newsgroups: bionet.diagnostics Subject: Rheumatiod factor antibody Date: 11 Mar 1998 09:24:30 -0800 Organization: INCSTAR Corporation Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6e6hce$986@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I'm Dr Zanin and I'd like to now where I can find some rheumatoid factor antibody. Thanks for your help From owner-diagnostics@net.bio.net Sun Mar 15 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Ibrahim Kamel Newsgroups: bionet.diagnostics Subject: Anaesthesia Date: 16 Mar 1998 02:12:12 -0800 Organization: Panasonic Infor. & Net Tech Laboratory Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6eitts$ru@net.bio.net> NNTP-Posting-Host: net.bio.net Does propofol produce cholestasis when it is used as a drip for long periods (3-5 days). Thanks for your help, ibrahim ibrahim@research.panasonic.com P.S.: If this is not the right place to post this question, please point me to the relevant newsgroup. From owner-diagnostics@net.bio.net Sun Mar 15 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Alex Chang Newsgroups: bionet.diagnostics Subject: PCR detection of tinea infection Date: 16 Mar 1998 02:03:14 -0800 Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6eitd2$sic@net.bio.net> NNTP-Posting-Host: net.bio.net I'd like to know whether PCR method can be applied to detect tinea (hands or feet). Thanks in advance Alex Chang Pathology University of British Columbia achang@hivnet.ubc.ca From owner-diagnostics@net.bio.net Wed Mar 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: xx Newsgroups: bionet.diagnostics Subject: Antimicrobial resistance TV program, 8pm March 19 Date: 19 Mar 1998 01:09:08 -0800 Organization: xx Lines: 66 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6eqnbk$av@net.bio.net> NNTP-Posting-Host: net.bio.net Just a note to those who are interested that 'Quantum', the ABC TV science program, will air an half-hour special on antibiotic resistance at 8pm on Thursday, March 19, 1998. The program talks to a number of researchers in the field, including Stuart Levy in Boston and John Turnidge in Adelaide, and takes a hard look at the animal link to the rise of resistance, Below is the synopsis from our website. Hope thos of you that are able to watch will. Sincerely, Wilson da Silva Journalist ABC TV Science Unit ---------------------------------------------------- ``PASSING THE BUG ... The end of antibiotics? 8.00 pm, Thursday March 19, 1998 We are running out of antibiotics. The microbes are fighting back, they have developed resistance to our most powerful biological weapons - and they are invading our hospitals. Already, microbes resistant to the most powerful and most toxic antibiotic, Vancomycin, have sprung up in European and American hospitals. Once in the bloodstream of seriously ill patients, they have a 60 per cent chance of dying. Doctors can do little else than watch and hope that a patient's own immune system will fight it off. In 1994, this microbe -- VRE -- arrived in Australia; there have been 60 cases around the country, six of them fatal. Doctors are so worried they are isolating patients with any hint of the bug, even if they are not very ill. In Japan, the doomsday scenario that had long been feared is emerging: the golden staph germ that used to plague hospitals and kill 80 per cent of its victims once in the bloodstream has returned. And it is resistant to just about every antibiotic, including Vancomycin. Within months, cases of the superbug sprang up in Michigan and New Jersey. Quantum talks to patients who have lived the nightmare of fighting resistant infections in Australia -- a man whose leg had to be amputated to try and stop a resistant golden staph infection; a woman who was infected with VRE and treated as a pariah by other hospitals, who would not take her. We look at the link between the use of antibiotics in animals and the rise of resistance in humans. Two thirds of antibiotics in Australia are used on livestock -- not to treat sick animals, but as growth promoters. Researchers now conclude that their widespread use in creating a reservoir of resistant bugs that then attack humans. The data convinced the European Union to ban come antibiotics as growth promoters, and now the World Health Organisation has come out against them. But Australian authorities deny there is a link. Microbiologists tell Quantum that the evidence is clear, the practice is madness, and a ban is needed. We talk to doctors who feel pressurized by patients to prescribe antibiotics, to nurses who deal with the rising number of infections day-to-day. Quantum will for the first time reveal the extent of the problem in Australia: how the number of infections is running at tens of thousands per year. It is a silent public health emergency. It may well be that, only 54 years after penicillin arrived to save us from scourges like tuberculosis and pneumonia, that we are now entering a world where we will have to do without antibiotics. c 1998 Australian Broadcasting Corporation From owner-diagnostics@net.bio.net Sun Mar 22 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Bjorn P." Newsgroups: bionet.diagnostics Subject: New and interesting site for antibodies. Date: 23 Mar 1998 06:32:14 -0800 Organization: Telenor Online Public Access Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6f5rpe$bfs@net.bio.net> NNTP-Posting-Host: net.bio.net There is a new and interesting site on the Net! Check out and bookmark: http://209.133.10.122/ DIATEC AS is offering high quality monoclonal antibodies at a reasonable price. They deliver direct to you by DHL and reply to your question within 24 hours. They also offer free samples of their products. A mice friendly supplier, since they produce all their mAbs in-vitro. Bjorn From owner-diagnostics@net.bio.net Sun Mar 22 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: Life science resource Date: 23 Mar 1998 08:31:04 -0800 Organization: BT Internet Lines: 7 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6f62o8$pjc@net.bio.net> NNTP-Posting-Host: net.bio.net I have found an ace web resource that provides product data and links to manuafcturers home sites at: http://www.corniche.com Jeff From owner-diagnostics@net.bio.net Mon Mar 23 22:00:00 1998 Path: biosci!biosci!not-for-mail From: David Croan Newsgroups: bionet.diagnostics Subject: PCR internal control Date: 24 Mar 1998 01:47:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6f7vev$kp9@net.bio.net> NNTP-Posting-Host: net.bio.net I would like to hear from anyone utilising or developing diagnostic PCRs. More specifically I would like your views on the need for an internal control and the nature of that control. I have inserted a synthetic fragment into a cloned PCR target which I include in my reaction. Obviously this is desirable to the extent that one can positively determine the success of the PCR e.g. whether or not a sample contains inhibitory agents etc. However in my experience, I have seen a quite dramatic decrease in sensitivity due to the competitive reaction. Also this makes colormetric detection using bio/strept. impossible. Thankyou for your comments From owner-diagnostics@net.bio.net Mon Mar 23 22:00:00 1998 Path: biosci!biosci!not-for-mail From: DADT2243 Newsgroups: bionet.diagnostics Subject: Re: Rheumatiod factor antibody Date: 24 Mar 1998 05:08:17 -0800 Organization: AOL http://www.aol.com Lines: 2 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6f8b81$e9q@net.bio.net> NNTP-Posting-Host: net.bio.net You can try Serologicals. They have plasma from individuals with a range of antibody levels. Try nmartin@serologicals.com , she maybe able to help you. From owner-diagnostics@net.bio.net Tue Mar 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "W. Kaminski" Newsgroups: bionet.diagnostics Subject: HELP: anti mouse macrophage/lymphocyte AB Date: 25 Mar 1998 01:28:56 -0800 Organization: University of Washington Lines: 15 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6faioo$ar7@net.bio.net> NNTP-Posting-Host: net.bio.net Hi: I am looking for an anti mouse macrophage antibody and also an anti mouse CD4/CD8 or, alternatively, pan-lymphocyte antibody that works in immunohistochemistry. I am aware that there are quite a few out there, however, the ones I am particularly interested in are antibodies that work in tissue that was fixed with an ethanol/glacial acidic acid/chloroform mix (such as eg in Carnoy's fixative). I'd appreciate any help/information/input on this matter. Thanks From owner-diagnostics@net.bio.net Tue Mar 24 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Daniel Saul Ore Chavez Newsgroups: bionet.diagnostics Subject: Fragments chromosomics in liquid ammiotic Date: 25 Mar 1998 01:29:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6faiqi$b1e@net.bio.net> NNTP-Posting-Host: net.bio.net Hello: Consult: The diagnose prenatal of liquid amniotico detects cariotipo 46, XY but is observed in all metafases analyzed two fragments that pretend to be satellite, we want to know if this associate to pathology chromosomics and that relations have of alteration presented/displayed the fetus. Dr. Mariela Flores Hospital Guillermo Almenara Irigoren Lima - Peru ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From owner-diagnostics@net.bio.net Mon Mar 30 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "DANIEL ORE[1000209@UNMSM.EDU.PE]" <1000209@unmsm.edu.pe> Newsgroups: bionet.diagnostics Subject: TEST LEUKEMIA IN PCR Date: 31 Mar 1998 02:24:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6fqg83$aar@net.bio.net> NNTP-Posting-Host: net.bio.net Hello: A consultation because in the leukemia tests where involved in gene brc (crom 22) and the gene c-abl (crom9), there is one formacion of a chimera, for I diagnose of this is used a test of RT-PCR, (transcriptasa reversa), my question is because it is not used as it sample the DNA of the patient and because the RNA, if it is that in the constitution of the DNA is the problem. Thank you Daniel Ore Chavez Universidad Nacional Mayor de San Marcos Facultad de Ciencias Biologicas E-mail: 1000209@unmsm.edu.pe