From owner-diagnostics@net.bio.net Thu Apr 02 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Apr 1998 02:30:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6g2dp0$8lu@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Sun Apr 05 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Rona Jones Newsgroups: bionet.diagnostics Subject: health screening systems Date: 6 Apr 1998 02:00:46 -0700 Organization: DreamsFaithfully Limited Lines: 15 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ga5ju$8i9@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for health screening systems that are either available in the UK and Europe or can be brought to those markets. The key requirements include:- 1 - a system to measure key body parameters while the patient is being examined 2 - the data are to be fed directly into the patient database for later analysis 3 - graphical "walk-thru" of the data 4 - recall systems to get the patient back for further checks every few years Details of any systems or further ideas must appreciated. -- Rona Jones - ruj@dreamsfaithfully.com From owner-diagnostics@net.bio.net Tue Apr 07 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: searchable science resources Date: 8 Apr 1998 04:22:41 -0700 Organization: BT Internet Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6gfmm1$j05@net.bio.net> NNTP-Posting-Host: net.bio.net I have located a free service for locating life science products at: http://www.corniche.com try it - there are on-line quote requests etc. Jeff Slater From owner-diagnostics@net.bio.net Tue Apr 07 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Hart, Alan" Newsgroups: bionet.diagnostics Subject: Protein foams Date: 8 Apr 1998 04:35:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6gfnej$k3f@net.bio.net> NNTP-Posting-Host: net.bio.net Dear All, I want to add some further components to fairly concentrated solutions of enzymes. In mixing everything together, I introduce air into the mixture. This causes the mixture to "foam" i.e. develop rather stable air bubbles which I assume are being stabilised by protein films. Can anyone suggest an additive to prevent these bubbles from forming (I want to avoid any any harsh methods for getting rid of them eg evacuating the mixture at low pressure)? Thank you. Alan Hart From owner-diagnostics@net.bio.net Mon Apr 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: g9711278@inhavision.inha.ac.kr Newsgroups: bionet.diagnostics Subject: PCR using yoghurt sample Date: 14 Apr 1998 02:13:56 -0700 Organization: Deja News - The Leader in Internet Discussion Lines: 25 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6gv9ck$1n3@net.bio.net> NNTP-Posting-Host: net.bio.net Hi~ I tried to amplify microbes' 16S rRNA gene from yoghurt sample, but I couldn't. For this, I treated plane yoghurt with ProteinaseK during 4hr and overnight at 37C. I found that almost proteins degraded when I centrifuged treated sample. I washed pellet twice with PBS and once with pure water. Finally, I added 1% Tween-80 solution and lysed cell by freeze and thaw lysis method(I evaluated this washing and lysis method before). Lysed sample was used directly to PCR. I couldn't found PCR product. To evaluate sample purity, I applied sample from yoghurt and pure culture(had used positive control) together to a PCR in a tube. No product. I think the sample from yoghurt contains some inhibitory materials. I don't know what material inhibits PCR. If you have some idea, could you help me? and... If you know how can you remove them, could you tell me that? Adios.. ---- Lab. of Genetic Engineering, Inha Univ., Inchon, South Korea. Youngmin Park bada@bigfoot.com ---- -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-diagnostics@net.bio.net Mon Apr 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Menoret Newsgroups: bionet.diagnostics Subject: hsp and immunity Date: 14 Apr 1998 02:23:32 -0700 Organization: @Home Network Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6gv9uk$36c@net.bio.net> NNTP-Posting-Host: net.bio.net Please note the International Congress on "Heat shock proteins in Immune Response "is to be held in Farmington (CT) USA, October 12-15, 1998. For information and registration details: http://www.hspimmunity.com/ Hope to see you there Antoine Menoret Ph.D. Center for Immunotherapy of Cancer and Infectious Diseases (MC1601) University of Connecticut School of Medicine Farmington, CT 06030-1601, USA Phone: (860) 679 4444 Fax: (860) 679 7905 E mail: menoret@up.uchc.edu From owner-diagnostics@net.bio.net Mon Apr 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: HIGH PERFORMANCE TAQ Date: 14 Apr 1998 05:13:24 -0700 Organization: BT Internet Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6gvjt4$kos@net.bio.net> NNTP-Posting-Host: net.bio.net Sources of High Performance TAQ and other reagents by searching the Corniche data base at http://corniche.com. This database lists 50,000 products and has complex search facilities - free to use! Jeff slater From owner-diagnostics@net.bio.net Thu Apr 16 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Valeria Maida Newsgroups: bionet.diagnostics Subject: NAIMI Congress Date: 17 Apr 1998 06:57:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 91 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6h7n3u$2d2@net.bio.net> NNTP-Posting-Host: net.bio.net It gives us great pleasure to inform you that the NAIMI Congress will be held in Alghero, on the North West of Sardinia, in the centre of Mediterranean Sea, this September as a satellite symposium to the Congress in Coordination Chemistry in Florence. This congress is specifically aimed at those working in the DNA Polymers field and promises to be a valuable and stimulating occasion. The opportunity for experts in the general chemical sector to meet those working on the base of DNA and RNA in an informal setting should lead to interesting brainstorming sessions, which will undoubtedly be of great use to both sides.
Yours,
Prof. M.L.Ganadu & Prof. M. Taddei - Dipartimento di Chimica - via Vienna, 2 - 07100 Sassari


UNIVERSITA DEGLI STUDI DI SASSARI
NAIMI
Nucleic acids and their interactions with metal ions


Satellite Symposium of the XXXIII International Conference
on Coordination Chemistry in Florence
Alghero September 5-7 1998

The mini symposium is intended for researchers in the Chemistry of Nucleic Acids and Metal Ions field. The main objective of the symposium is to bring together scientists interested in the different aspects of the influence of metal ions on the structure and metabolism of DNA. The main topics will be DNA Polymers, nucleic metal proteins, zinc fingers and PNA.

Scientific Program:
The following distinguished speakers have already agreed to deliver a lecture:
Prof. James Allan Cowan (COLUMBUS, USA),
Prof. Rob Kaptein (UTRECHT, THE NETHERLANDS),
Prof. Bernard Meunier (CNRS, FRANCE),
Prof. Thomas W. Myers (ROCHE, USA),
Prof. Huguette Pelletier (HOUSTON, USA).
Prof. Bibudgendra Sarkar (TORONTO, CANADA),
In addition there will be a poster session and a number of selected oral communications. All those who wish to participate are invited to submit an abstract to the scientific committee.

Organising committee:
F. Bonomi (University of Milano - Italy)
M.L. Ganadu, Chairperson (University of Sassari - Italy)
H. Kozlowski (University of Wrocklaw - Poland)
C. Mealli (CNR, Florence - Italy)
A. Scozzafava (University of Florence - Italy)
M. Taddei (University of Sassari - Italy)

additional information and a registration form is available on:
http://www.uniss.it/web/congressi/naimi/naimiaw.htm

For further information, please contact:
Peter Norton peter.norton@flashnet.it phone/fax: +39 79 299640
Naimi secretary naimi@ssmain.uniss.it fax +39 79 229559 or 229482
Newtours Evangelist@Mail.Newtours-CMO.it Fax+3955/3361250/350
*********************************************************************
Valeria Maida          Tel. + 39 79 229542/229588     
Dipartimento di Chimica     fax  + 39 79 229559/229482    
via Vienna, 2          e-mail billia@ssmain.uniss.it   
07100 Sassari               &nbs ;             
********************************************************************* Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Tel 0131 244 8924 Edinburgh Fax 0131 244 8926 EH12 8NJ UK From owner-diagnostics@net.bio.net Mon Apr 20 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: FREE LIFE SCIENCE RESOURCE Date: 21 Apr 1998 04:18:50 -0700 Organization: BT Internet Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6hhvaq$ai1@net.bio.net> NNTP-Posting-Host: net.bio.net A comprehensive data base of antibodies, antigens and many other life science products and services is located at:- http://www.corniche.com Marion Parker From owner-diagnostics@net.bio.net Thu Apr 30 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Bryan Grieg Fry Newsgroups: bionet.diagnostics Subject: new WWW board - updated Australian Venom & Toxin Database Date: 1 May 1998 07:59:40 -0700 Organization: 3D Centre, University of Queensland Lines: 32 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ico0s$j3f@net.bio.net> NNTP-Posting-Host: net.bio.net The Australian Venom and Toxin Database has been recently updated. The most significant addition being a new www based bulletin board. The purpose of this group is to be an interactive forum in order to provide an online scientific discussion of Australian venomous snakes and other venomous animals for researchers and devoted amateurs. The focus is primarily on the venom and the clinical effects of the venom but relevant taxonomical discussions are appropriate. Discussion of non-Australian venoms or toxins will also be allowed. For a discussion of non-venom related topics concerning non-Australian venomous animals please go to http://www.kingsnake.com/forum/venom. For general care and feeding of reptiles please visit the rec.pets.herp newsgroup. With regards, Bryan Grieg Fry ----------------- Australian Online Venom & Toxin Database http://www.uq.edu.au/~ddbfry/index.html Peptide Laboratory Centre for Drug Design & Development University of Queensland St. Lucia, Qld 4072 Australia email: b.fry@mailbox.uq.edu.au Fax: (61) 7 3365 1990 Phone (61) 7 3365 1271