From owner-diagnostics@net.bio.net Mon May 04 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 5 May 1998 02:14:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6imlaf$shf@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Larry Winger Newsgroups: bionet.diagnostics Subject: Discrimination between Human rInsulin and endogenous Insulin Date: 6 May 1998 04:59:01 -0700 Organization: Clinical Biochemistry, University of Newcastle upon Tyne Lines: 10 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ipja5$gm0@net.bio.net> NNTP-Posting-Host: net.bio.net An obvious question, perhaps, but I wonder whether standard assays for insulin can discriminate between recombinant insulin and endogenous material? Perhaps somebody can point me towards an antibody that recognises one but not the other? Many thanks Larry Winger From owner-diagnostics@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Polymerase Chain Reaction Discussion Newsgroups: bionet.diagnostics Subject: New PCR Forum Date: 6 May 1998 01:01:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ip5cm$krp@net.bio.net> NNTP-Posting-Host: net.bio.net A new website on PCR. A new interactive site -- http://sunsite.berkeley.edu/pcr -- devoted to discussion and debate about the past, present, and future of PCR (polymerase chain reaction) technology. Features a digital archive of foundational articles, a brief introduction, and regular presentations on recent advances in PCR research and development. Soren Germer, Ph.D. University of California at Berkeley From owner-diagnostics@net.bio.net Wed May 06 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Ksprasad Newsgroups: bionet.diagnostics Subject: APT and CNBr activated paper Date: 7 May 1998 03:24:32 -0700 Organization: AOL http://www.aol.com Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6is250$lp1@net.bio.net> NNTP-Posting-Host: net.bio.net Would like to know how to make (or where to buy) APT or CNBr activated paper for binding proteins. S&S does not sell APT paper anymore. From owner-diagnostics@net.bio.net Wed May 06 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Craig Webster Newsgroups: bionet.diagnostics Subject: Re: Discrimination between Human rInsulin and endogenous Insulin Date: 7 May 1998 05:51:52 -0700 Organization: Biochemistry Department, Broomfield Hospital Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6isap8$9fb@net.bio.net> NNTP-Posting-Host: net.bio.net In article <6ipja5$gm0@net.bio.net>, Larry Winger writes >An obvious question, perhaps, but I wonder whether standard assays for >insulin can discriminate between recombinant insulin and endogenous >material? > The usual way is to measure a c-peptide at the same time as the insulin. As you probably know c-peptide is produced from the cleavage of pro- insulin which does not occur with recombinant insulin. -- Craig Webster |Tel: 01245 514013 Senior Clinical Biochemist |Fax: 01245 514077 Broomfield Hospital |email: cwebster@broombio.demon.co.uk Chelmsford, Essex, UK |http://www.broombio.demon.co.uk/ From owner-diagnostics@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Dave Jensen Newsgroups: bionet.diagnostics Subject: Bio Online Career Discussion Forum, Mentors Needed! Date: 11 May 1998 01:08:59 -0700 Organization: Search Masters International Lines: 44 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6j6bmr$b6v@net.bio.net> NNTP-Posting-Host: net.bio.net The Career Discussion Forum at Bio Online is one of the most active online discussion forums covering the world of science careers, biotechnology, and pharmaceuticals. This forum has hundreds of archived and current posts related to topics about careers: various bench science and non-bench science positions, alternative careers, and job search related discussion. You will find it of interest whether you are just starting out or whether you are advancing on a senior-level career track. The location is: http://www.bio.com/hr/forum/ Although the forum has been very successful to date, we are constantly thinking about ways to improve it. One of the ways to do this is will be to add new voices in specialized areas when questions arise about these fields. Currently, we have a number of experts who are available in certain career areas as Mentors. But we need more! We are currently recruiting for senior level professionals to join us as Mentors in the following fields: Clinical Research, Sales and Marketing, Business Development, Regulatory Affairs, Patent Law, Finance, and in several unique categories of bench science (diagnostics, combinatorial chemistry, genomics). If you are already a contributor in your field of expertise, we thank you. Our forum has been very lucky to have a number of very WONDERFUL frequent posters (you know who you are -- my heartfelt thanks). But -- there are a great number of specialty career areas in the world of biotechnology and I seek to find even more regular posters in certain areas. Every Mentor who is registered with us on a particular topic will be alerted by email when we have a post on the forum which falls into their listed field of expertise. I can guarantee that the program will not overburden you, and the average involvement may be no more than 15 minutes a month. But just think about the great need that others have for your useful career information, and how grateful the group will be to receive it! We can post your messages for you, anonymously, if you wish -- but you may find that the interactive nature of this wonderful forum stimulates both those asking the questions as well as the Mentor. Your recommendations would sure be appreciated in personal email directed to my attention at SMI@sedona.net, if you have suggestions or interest. Please join us for a visit or an online question about biotechnology careers! Best regards, Dave Jensen, Moderator, Bio Online Career Discussion Forum From owner-diagnostics@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "James J. Campanella" Newsgroups: bionet.diagnostics Subject: cuvette question Date: 11 May 1998 01:13:26 -0700 Organization: Case Western Reserve University, Cleveland, OH (USA) Lines: 22 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6j6bv6$bgf@net.bio.net> NNTP-Posting-Host: net.bio.net Dear netters, I have what may be considered a silly question. We all know that in looking at UV absorbance in a spectrophotometer that we use quartz cuvettes because glass absorbs UV light and doesn't give you an accurate answer. But, can you use quartz cuvettes in looking at the white light absorbance of something in a spec? In other words, does a lab need both a set of glass AND quartz cuvettes? I have not been able to come up with a good reason why not. Any comments? Thanks, Jim Campanella Lehigh University Dept. of Biological Sciences -- **The Past tempts us, the present confuses us, and the future frightens us . . . and our lives slip away, moment by moment, lost in that vast terrible in-between** -The late Centauri Emperor From owner-diagnostics@net.bio.net Mon May 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Peter R David Newsgroups: bionet.diagnostics Subject: Re: cuvette question Date: 12 May 1998 01:32:19 -0700 Organization: Stanford University Lines: 15 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6j91ej$ojh@net.bio.net> NNTP-Posting-Host: net.bio.net Quartz cuvettes have a broader range of transparency than glass. You may still want to have more than one set of cuvettes given the cost of the quartz cuvettes. Depending on what you are looking at, plastic cuvettes may be a viable alternative. Good luck, Peter David -- Peter R. David, Ph. D. D-100 Fairchild Bldg Tel 650-725-0754 Stanford University Fax 650-723-8464 Stanford, CA 94305-5400 From owner-diagnostics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jeff Slater Newsgroups: bionet.diagnostics Subject: directory of life science products Date: 14 May 1998 06:18:49 -0700 Organization: BT Internet Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6jeqvp$8u4@net.bio.net> NNTP-Posting-Host: net.bio.net I have located a free service for locating Life Science Products at: http://www.corniche.com Marion Parker From owner-diagnostics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jason Alter Newsgroups: bionet.diagnostics Subject: Diagnostic Newsletters Date: 14 May 1998 01:27:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6je9ua$7nl@net.bio.net> NNTP-Posting-Host: net.bio.net Pall Specialty Materials, a division of the Pall Corporation has started two free, electronic newsletters. The newsletters are bimonthly. The first newsletter focuses on one-step immunodiagnostics and the second focuses on molecular diagnostics/high throughput screening. Both will contain technical tips, protocols and the most recent information on membranes for diagnostics. If you are interested in receiving either or both of these newsletters please send a request to Jason_Alter@Pall.com. Thank you for your interest. From owner-diagnostics@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Glen Ramsay Newsgroups: bionet.diagnostics Subject: Re: cuvette question Date: 14 May 1998 09:06:46 -0700 Organization: Aviv Instruments Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6jf4qm$1np@net.bio.net> NNTP-Posting-Host: net.bio.net Get a copy of the Helma catalog. They have tranmission profiles over broad wavlength ranges for their glasses. Glen In article <6j6bv6$bgf@net.bio.net>, "James J. Campanella" wrote: > Dear netters, I have what may be considered a silly question. We > all know that in looking at UV absorbance in a spectrophotometer > that we use quartz cuvettes because glass absorbs UV light and > doesn't give you an accurate answer. But, can you use quartz > cuvettes in looking at the white light absorbance of something > in a spec? In other words, does a lab need both a set of glass > AND quartz cuvettes? I have not been able to come up with a good > reason why not. Any comments? > > Thanks, > > Jim Campanella > Lehigh University > Dept. of Biological Sciences From owner-diagnostics@net.bio.net Thu May 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: kar-boulhimez@wanadoo.fr Newsgroups: bionet.diagnostics Subject: need used kodak ektachem Date: 15 May 1998 08:02:44 -0700 Organization: Wanadoo - (Client of French Internet Provider) Lines: 17 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6jhlek$8hq@net.bio.net> NNTP-Posting-Host: net.bio.net I'm looking for one to three kodak 500,700 or even 250 . I'm located in france but no matter the instrument comes from . Many thanks for you replies at : kar-boulhimez@wanadoo.fr begin 666 k.boulhimez.vcf M0D5'24XZ5D-!4D0-"E9%4E-)3TXZ,BXQ#0I..F)O=6QH:6UE>CMK+@T*1DXZ M:RYB;W5L:&EM97H-"D5-04E,.U!2148[24Y415).150Z:V%R+6)O=6QH:6UE M>D!W86YA9&]O+F9R#0I2158Z,3DY.# U,354,30T-# W6@T*14Y$.E9#05)$ "#0H` ` end From owner-diagnostics@net.bio.net Thu May 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Thomas Berg Newsgroups: bionet.diagnostics Subject: In vitro allergy diagnostics in Europe Date: 15 May 1998 07:46:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6jhkfk$66q@net.bio.net> NNTP-Posting-Host: net.bio.net For a market study on allergy test systems, I am looking for names and addresses of companies who offer in vitro allergy diagnostics tests in European countries. If you know any or even are one yourself, please send an e-mail to berg.bmd@t-online.de Thank you From owner-diagnostics@net.bio.net Sun May 17 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell Newsgroups: bionet.diagnostics Subject: :Biochemistry (Moscow) on-line available Date: 18 May 1998 09:20:10 -0700 Organization: Scottish Agricultural Science Agency Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6jpn3q$3dm@net.bio.net> NNTP-Posting-Host: net.bio.net Subject:Biochemistry (Moscow) on-line available To: bionet-diagnostics@net.bio.net From: "Emir KHATIPOV" Date: Wed, 13 May 1998 18:02:41 +0900 Message-ID: <6jbnlq$fup$1@rpnntp.aist.go.jp> Return-Path: Biochemistry (Moscow), an official English translation of Biokhimiya, is available on-line with full text of almost all articles. http://www.protein.bio.msu.su/biokhimiya/index.htm ------------------------------------------------------------- From owner-diagnostics@net.bio.net Thu May 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Cynthia S. Smagula" Newsgroups: bionet.diagnostics Subject: 100 New Tools Added to BioToolKit Date: 29 May 1998 01:17:38 -0700 Organization: BIOTA Publications Lines: 29 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6klqv2$4qg@net.bio.net> NNTP-Posting-Host: net.bio.net The BioToolKit, an annotated directory of over 600 online molecular biology resources (at http://www.biosupplynet.com) has recently added links to many new resources, including: ACUTS (Ancient Conserved Untranslated Sequences) dbCFC (Cytokine Family database) DNA Patent Database CD Guides (cell surface markers) MAR-Finder (Matrix Attachment Regions) In Situ PCR on Plant Material Vacuum Infiltration Transformation of Arabidopsis PPMdb (Plant Plasma Membrane database) MitBASE (comprehensive mitochondrial database) GenProtEC (E.coli gene relationships) The Interactive Fly WormPep (predicted proteins in C.elegans) WebMolecules VRML Player (analyzes your system, suggests viewers) 3-D Crunch (SWISS-MODEL predicts 50,000 structures) Atlas of Protein Topology Cartoons (simple 2-D diagrams) Movies of Protein Motions Protein Morphing Server PROMISE: Prosthetic Groups and Metal Ions in Protein Active Sites ADOPS: Associative Database of Protein Sequences NeuronDB Complete descriptions of these sites and links are found at http://www.biosupplynet.com, just click on the BioToolKit. The BioToolKit database is designed for rapid page delivery, functionally organized, and Verity-searchable. From owner-diagnostics@net.bio.net Thu May 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Rimantas Kodzius Newsgroups: bionet.diagnostics Subject: Oligos from cDNA - from which region? Date: 29 May 1998 02:17:38 -0700 Organization: Max-Planck-Institute for Molecular Genetics Lines: 20 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6klufi$cg2@net.bio.net> NNTP-Posting-Host: net.bio.net Halloo! Can somebody help me? I have many cDNA's from A.fumigatus. I want to choose representing oligos for every cDNA for hybridization. The oligos should be representative for every cDNA, and recognize just the same cDNA clone. I should avoid DNA sequence from conserved domains. Do You know some program, which would be suitable for this purpose? Thank You very much! Rimantas.Kodzius@sbg.ac.at