From owner-diagnostics@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Jun 1998 02:27:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6l34tj$gf5@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Mark Cauchi Newsgroups: bionet.diagnostics Subject: Recombinant DNA Techniques Course Date: 4 Jun 1998 01:29:11 -0700 Organization: Monash University Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6l5lsn$c5q@net.bio.net> NNTP-Posting-Host: net.bio.net The Micromon Unit at the Department of Microbiology, Monash University in Melbourne, Australia will be running its Recombinant DNA Techniques Course between the 15-20 November, 1998. This is an introductory-intermediate level course which offers a skills-based training package. If you would like further information, details can be found on our web page at http://www.med.monash.edu.au/micro/department/dnacorse.htm From owner-diagnostics@net.bio.net Thu Jun 18 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Shammas Newsgroups: bionet.diagnostics Subject: mRNA and Taq polymerase Date: 19 Jun 1998 02:33:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mdb9l$fgl@net.bio.net> NNTP-Posting-Host: net.bio.net Why does Taq polymerase work only on cDNA and not mRNA? From owner-diagnostics@net.bio.net Thu Jun 18 23:00:00 1998 Path: biosci!biosci!not-for-mail From: RSA Newsgroups: bionet.diagnostics Subject: POSITION: Statistician/Systems Scientist Date: 19 Jun 1998 03:19:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mddv6$iqf@net.bio.net> NNTP-Posting-Host: net.bio.net Statistician/Systems Scientist Position Description: We are seeking a statistician for the characterization and evaluation of sophisticated instrument systems in the medical diagnostics industry. Responsibilities: =B7 Work with engineers and scientists to develop and execute sophisticated designed experiments. =B7 Creation and maintenance of a SAS Macro library =B7 Design experiments that quantify sources of variability and lead to improvements in system performance. Qualifications =B7 Read/convert data from various sources (text files, Oracle databases, ODBC). =B7 Ideally, familiar with the characterization and evaluation of sophisticated instrument systems in the diagnostics industry. =B7 Familiar with statistical analysis and interpretation of complex data sets. =B7 Solid working knowledge and experience in the application of statistical techniques (t-tests, ANOVA, Design of Experiments, Boot-strapping, etc.) If you have an interest in this or other opportunities, please send us your resume/CV as an Attached File to an email or send by mail/FAX to RS&A to the attention of Ann Rathbun, Managing Director. All correspondence is held in strict confidence. Rathbun, Sapir & Associates P.O. Box 2337 Sedona, AZ 86339-2337 * USA (520) 203-0074 Office (520) 203-0075 FAX E-mail: rathbun@sedona.net From owner-diagnostics@net.bio.net Thu Jun 18 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Shammas Newsgroups: bionet.diagnostics Subject: amplitagold Taq polymerase Date: 19 Jun 1998 02:33:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mdb92$fd9@net.bio.net> NNTP-Posting-Host: net.bio.net I noticed that using amplitagold Taq polymerase in PCR gives a double band of the amplicon but not with ordinary Taq polymerase. Has anybody experienced anything similar and what may be the explanation ? From owner-diagnostics@net.bio.net Sun Jun 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Gunnaporn Veerachato Newsgroups: bionet.diagnostics Subject: Q:The eradication program of CSFV? Date: 22 Jun 1998 01:01:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ml312$j2u@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all I have heard that Denmark is one of the classical swine fever virus (CSFV) free area. Could anyone please tell me the diagnostic methods and eradication program of CSFV in Denmark? Thanks in advance Gunnaporn Veerachato From owner-diagnostics@net.bio.net Sun Jun 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: naimi secretary Newsgroups: bionet.diagnostics Subject: NAIMI congress Date: 22 Jun 1998 08:47:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mluad$cm1@net.bio.net> NNTP-Posting-Host: net.bio.net Dear,

The University of Sassari, Sardinia, Italy, supports initiatives centred around the theme of scientific research in the framework of EU policies promoting transnational scientific co-operation among European and other countries. In particular, the University of Sassari is organising a round table entitled:

Scientific research in Mediterranean Countries in the framework of EU programmes:
current status and perspectives

The round table will take place on September 5th at the "Porto Conte Ricerche" centre, (Alghero, SS, Sardinia), as part of the NAIMI symposium (Nucleic Acids and their Interactions with Metal Ions), a satellite symposium of the ICCC Congress (International Conference on Coordination Chemistry - Florence).

We would be very glad if a representative of your Agency/Organisation would be interested in participating in the round table; we believe that this event will provide a good opportunity to exchange information on EU policies for the support of international research projects, and possibly offer some ideas on forms of co-operation between EU and non EU countries.

In order to further promote closer links between the world of research and Public Support Agencies, we are also forwarding an invitation to main Universities/Research Institutes from various countries to submit applications by a young scientist (under 35 years of age)to participate in the works of the NAIMI symposium.

We are looking forward to your kind reply, and are at your disposal for any further information you may request.

Yours sincerely

Prof. Maria Luisa Ganadu


For further information about NAIMI Congress, please contact naimi@ssmain.uniss.it
From owner-diagnostics@net.bio.net Mon Jun 22 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Cramoy Newsgroups: bionet.diagnostics Subject: Accuracy of T-cell stimulation test Date: 23 Jun 1998 01:37:41 -0700 Organization: AOL http://www.aol.com Lines: 29 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mnpgl$p33@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for information on the accuracy, or lack thereof, of T-cell stimulation tests (mitogen stimulation tests) specifically for the detection of Borrelia burgdorferi (causal agent in Lyme disease). I have done a literature search of the abstracts, but am looking for specific info on 1. Cross-reactivity with other spirochetes (With which spirochetes is it likely to cross-react? How frequent is this cross-reactivity, etc.), 2. Does test cross-react with non-spirochetal bacteria, (if so which ones and any theories on why)? 3. What are estimated numbers for the occurrence of other spirochetes in the general population? 4. Are there specific antigen tests to rule out infection by other spirochetes that may cross-react? And, if so, are these antigen tests anymore reliable than the ones currently available for Borrelia burgdorferi? 5. What are the chances that a test result more than 3 standard deviations above the norm for Borrelia burgdorferi is really due to cross-reactivity and has no significance in diagnosing Lyme disease? Does the usual meaning of standard deviations get thrown out the window due to cross-reactivity in this case? I would appreciate personal insights from researchers and laboratory personnel familiar with this topic, literature references that are worth reading in their entirety rather than as abstracts, references for textbooks or monographs that will contain detailed information that will help to sort out these questions. Thank you, Carolyn Cramoy, M. S. From owner-diagnostics@net.bio.net Wed Jun 24 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Yokhom Shasketper Newsgroups: bionet.diagnostics Subject: Chlamydia/S.abortusovis antibodies Date: 25 Jun 1998 08:03:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mtor5$41@net.bio.net> NNTP-Posting-Host: net.bio.net Hi! We are going to collect ovine sera from different animals vaccinated with an ovine Chlamydia - Salmonella abortusovis vaccine for a practice work on immunological responses. Although our old bi liographic protocols suggest the complement fixation test to discover antibody levels in vaccinated-sheep, we don't know a supplier for ovine sera (positive control) against Chlamydia or S.abortusov s (to be used in complement fixation test). Moreover, these protocols need antigen standardisation. We believe ELISA test in routine will be the best assay to replace the CF' test for Chlamydia antibody (there are several ELISA kits in the market). Are there any correlation between the CF' titre and the ELISA titre?. Our decision about S.abortus is more difficult. Tube or slide agglutination test are very easy to do, but what kind of purified antigen we have to use on routine tests (the antigenic formulae of S abortusovis is 4,12:c:1,6). Moreover, Have these tests enough sensibility to quantify an inmunological response by vaccination?. Is it possible to establish an titre of inmunological status? Could anyone suggest us any test for our investigation?. We would appreciate any comment. Thanks a lot in advance, Yokhom Shasketper __________________________________________________________ Get Your FREE Email address at http://www.GNWmail.com/ GNWmail.com - International free e-mail in your language From owner-diagnostics@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: wis-useco@usa.net Newsgroups: bionet.diagnostics Subject: Chlamydia/S.abortusovis antibodies Date: 26 Jun 1998 01:30:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mvm7c$re@net.bio.net> NNTP-Posting-Host: net.bio.net Hi! We are going to collect ovine sera from different animals vaccinated with an ovine Chlamydia - Salmonella abortusovis vaccine for a practice work on immunological responses. Although our old bi liographic protocols suggest the complement fixation test to discover antibody levels in vaccinated-sheep, we don't know a supplier for ovine sera (positive control) against Chlamydia or S.abortusov s (to be used in complement fixation test). Moreover, these protocols need antigen standardisation. We believe ELISA test in routine will be the best assay to replace the CF' test for Chlamydia antibody (there are several ELISA kits in the market). Are there any correlation between the CF' titre and the ELISA titre?. Our decision about S.abortus is more difficult. Tube or slide agglutination test are very easy to do, but what kind of purified antigen we have to use on routine tests (the antigenic formulae of S abortusovis is 4,12:c:1,6). Moreover, Have these tests enough sensibility to quantify an inmunological response by vaccination?. Is it possible to establish an titre of inmunological status? Could anyone suggest us any test for our investigation?. We would appreciate any comment. Thanks a lot in advance, Yokhom Shasketper ____________________________________________________________________ Get free e-mail and a permanent address at http://www.netaddress.com/?N=1 From owner-diagnostics@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Wjd4u Newsgroups: bionet.diagnostics Subject: EKG Date: 26 Jun 1998 01:31:04 -0700 Organization: AOL http://www.aol.com Lines: 1 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mvm88$s2@net.bio.net> NNTP-Posting-Host: net.bio.net I need information on the absence of R-wave on an EKG. From owner-diagnostics@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: mylove502@my-dejanews.com Newsgroups: bionet.diagnostics Subject: Re: mRNA and Taq polymerase Date: 26 Jun 1998 02:39:21 -0700 Organization: Deja News - The Leader in Internet Discussion Lines: 17 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mvq89$899@net.bio.net> NNTP-Posting-Host: net.bio.net You have to use Reverse Transcriptase because it is the only enzyme to synthesize cDNA from mRNA. If you want to amplify DNA from mRNA, you will use two step reactions. First, you will get cDNA from reverstranscription using RTase and then you can amplify numerous DNA molecules using PCR. Taq DNA polymerase is used in this step. From: Shammas Newsgroup: bionet.diagnostics Date: 1998? 6? 19? ??? PM 6:33 Subject: mRNA and Taq polymerase >Why does Taq polymerase work only on cDNA and not mRNA? -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-diagnostics@net.bio.net Thu Jun 25 23:00:00 1998 Path: biosci!biosci!not-for-mail From: AMBID Newsgroups: bionet.diagnostics Subject: M.Sc. Applied Molecular Biology of Infectious Diseases Date: 26 Jun 1998 02:38:52 -0700 Organization: LSHTM Lines: 27 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6mvq7c$88h@net.bio.net> NNTP-Posting-Host: net.bio.net MSc in the Applied Molecular Biology of Infectious Diseases London School of Hygiene and Tropical Medicine Applications are invited for entry to this course in September 1998. Minimal entry requirements are of a class 2:2 degree or appropriate training and experience. The course provides a thorough understanding and practical experience of molecular biology for students who wish to use this approach in clinical diagnosis, epidemiological investigations, or laboratory research on infectious diseases. A high proportion of students subsequently enter PhD programmes. For more information and an application form contact: The Deputy Registrar, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT. Telephone: +44 (0) 171 927 2239. Fax: +44 (0) 171 323 0638. Email: registry@lshtm.ac.uk Enquiries for course organiser: Email: m.miles@lshtm.ac.uk; Telephone: +44 (0) 171 927 2249/2639; Fax: +44 (0) 171 636 8739. M. A. Miles The School is an exempt charity dedicated to providing excellence in teaching, learning and research for the benefit of health worldwide, and is an equal opportunities employer From owner-diagnostics@net.bio.net Sun Jun 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Pieter van Santen Newsgroups: bionet.diagnostics Subject: The Main Metabolic Pathways Date: 29 Jun 1998 00:47:35 -0700 Organization: World Access Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6n7gqn$big@net.bio.net> NNTP-Posting-Host: net.bio.net Dear colleague, If you are interested in The Main Metabolic Pathways on the Internet, visit: http://home.wxs.nl/~pvsanten/mmp/mmp.html Good luck Pieter From owner-diagnostics@net.bio.net Sun Jun 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Cole Owen Newsgroups: bionet.diagnostics Subject: RNA Probes Date: 29 Jun 1998 08:45:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6n8cqr$6ll@net.bio.net> NNTP-Posting-Host: net.bio.net snip . . .from Howie Rose: >Has anybody had experience of using RNA probes for the detection of pathogenic bacteria without an amplification system? ------------ Isn't that the basis of the initial (and currently available) GenProbe manufactured commercial "DNA" probe tests - going for RNA of the subject pathogens rather than DNA? Their combination GC/Chlamydia test has been well accepted in clinical lab settings, along with other probe-based assays that they make using a similar "RNA" test principle, I believe. No amplification is used other than the "natural" application for those assays. (They do have an amplifications system, but amplification is not utilized for the subject assays.) Cole Owen Owen & Associates, Inc. Consultants to the Healthcare Industry 1233 Camino Del Mar Del Mar, California, USA phone (vox) 619-481-5991 phone (fax) 619-481-1741 email: cowen@electriciti.com From owner-diagnostics@net.bio.net Sun Jun 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Howie Rose Newsgroups: bionet.diagnostics Subject: RNA Probes Date: 29 Jun 1998 00:39:05 -0700 Organization: Virgin Net Usenet Service Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6n7gap$a6i@net.bio.net> NNTP-Posting-Host: net.bio.net Hi, Has anybody had experience of using RNA probes for the detection of pathogenic bacteria without an amplification system? As there are many copies of RNA floating around a cell, I would have thought that a detection system with the appropriate sensitivity highlight these without having to amplify as is necessary with DNA. Thanks Howie From owner-diagnostics@net.bio.net Tue Jun 30 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Robert Negm Newsgroups: bionet.diagnostics Subject: StrainMan Date: 1 Jul 1998 01:19:08 -0700 Organization: Caesar Software, LLC Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ncrds$rlp@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I am eager to receive feedback regarding our microbial database, called StrainMan 1.0, from www.caesarsoftware.com. Can you please post your comments about our database on our on-line forum at http://www.caesarsoftware.com/forum_frm.htm. Feel free to post any gripes, praise, or constructive criticisms. At Caesar Software, we are trying to make the best microbial database ever developed. Thanks again for everyone's support. Sincerely, Robert Negm, PhD Scientific Advisor, Caesar Software LLC negm@caesarsoftware.com http://www.caesarsoftware.com