From owner-diagnostics@net.bio.net Wed Sep 02 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Sep 1998 02:31:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6slnlf$c4o@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Sun Sep 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: fasahat@yahoo.com Newsgroups: bionet.diagnostics Subject: Pathnet Date: 14 Sep 1998 01:47:29 -0700 Organization: Deja News - The Leader in Internet Discussion Lines: 17 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6til71$gm5@net.bio.net> NNTP-Posting-Host: net.bio.net Pathnet ia a new interactive site for pathologists and lab technologists. Features include a discussion forum, conference calender, news, employment and numerous links. Links are being updated daily. Please send us your conference information, news and links. I invite you to suggest an interesting article you recently read in a journal or newspaper. http://members.tripod.com/~Pathnet Visit our conference calender where already CAP, ASCP, AFIP and several other conferences have been posted with links. Fasahat Wasty MD -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/rg_mkgrp.xp Create Your Own Free Member Forum From owner-diagnostics@net.bio.net Mon Sep 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell Newsgroups: bionet.diagnostics Subject: DNA isolation from fungal spores Date: 15 Sep 1998 04:05:05 -0700 Organization: Scottish Agricultural Science Agency Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tlhl1$6in@net.bio.net> NNTP-Posting-Host: net.bio.net Can anyone suggest a good (i.e. rapid, simple) method for isolating DNA from fungal spores? The quality of DNA has only to be good enough for PCR or LCR ampliciation, rather than cloning, orsequencing. Replies to the list please, rather than private email. Thanks, Kevin Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Tel 0131 244 8924 Edinburgh Fax 0131 244 8926 EH12 8NJ UK From owner-diagnostics@net.bio.net Mon Sep 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Pete Kwasowski Newsgroups: bionet.diagnostics Subject: Re: Patent database Date: 15 Sep 1998 05:54:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tlo2s$lb8@net.bio.net> NNTP-Posting-Host: net.bio.net Try: http://patents.uspto.gov/ "This page is the starting point for the USPTO's free patent databases. These include both the US Patent Bibliographic Database (a freely searchable database of front page information from US patents issued from 1/1/76 to 9/8/98 ), and the AIDS Patent Database (a freely searchable database of the full text and images of AIDS related patents issued by the US, Japanese and European patent offices)". Have fun! Pete At 04:06 15/09/98 -0700, you wrote: >I am looking for a searchable, on-line patent database. Can anyone >suggest any useful URLs? > >Replies to the list please. > >Thanks, > >Kevin >Dr Kevin O'Donnell >Diagnostics and Molecular Biology >Scottish Agricultural Science Agency Tel 0131 244 8924 >Edinburgh Fax 0131 244 8926 >EH12 8NJ UK > > > > From owner-diagnostics@net.bio.net Mon Sep 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Douglas Rhoads Newsgroups: bionet.diagnostics Subject: Re: DNA isolation from fungal spores Date: 15 Sep 1998 07:11:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tlsiq$2uc@net.bio.net> NNTP-Posting-Host: net.bio.net We had pretty good luck just boiling spore suspensions in TE. We got good sensitivity down to somewhere around 4-5 spores/PCR for Magneporthe grisea. On 15 Sep 98 at 12:09, Kevin O'Donnell wrote: > Can anyone suggest a good (i.e. rapid, simple) method for isolating > DNA from fungal spores? The quality of DNA has only to be good enough for > PCR or LCR ampliciation, rather than cloning, orsequencing. > > Replies to the list please, rather than private email. > > Thanks, > > Kevin > Dr Kevin O'Donnell > Diagnostics and Molecular Biology > Scottish Agricultural Science Agency Tel 0131 244 8924 > Edinburgh Fax 0131 244 8926 > EH12 8NJ UK > > PLEASE NOTE THAT MY EMAIL HAS CHANGED TO DRHOADS@COMP.UARK.EDU ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ ========================================================== "Strong and Bitter Words Indicate a Weak Cause." From a Chinese Fortune Cookie ========================================================== Douglas Rhoads, Assc. Professor & Chair Dept. of Biological Sciences, University of Arkansas drhoads@comp.uark.edu, http://comp.uark.edu/~drhoads 501-575-3251 From owner-diagnostics@net.bio.net Mon Sep 14 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell Newsgroups: bionet.diagnostics Subject: Patent database Date: 15 Sep 1998 04:06:04 -0700 Organization: Scottish Agricultural Science Agency Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tlhms$6so@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a searchable, on-line patent database. Can anyone suggest any useful URLs? Replies to the list please. Thanks, Kevin Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Tel 0131 244 8924 Edinburgh Fax 0131 244 8926 EH12 8NJ UK From owner-diagnostics@net.bio.net Tue Sep 15 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Buzby, Philip" Newsgroups: bionet.diagnostics Subject: RE: Searchable Patent Database Date: 16 Sep 1998 05:04:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6to9gc$7v3@net.bio.net> NNTP-Posting-Host: net.bio.net For DNA specific patents, try: http://208.201.146.119 Philip R. Buzby, Ph.D. Nonrad. Nucleotide R&D NEN Life Science Products, Inc. PO BOX 199151 Boston, MA 02119 Phone: 617-350-9343 Voice Mail: 800-446-0035, 1, 9343 FAX: 617-350-9658 E-Mail: buzbypr@nenlifesci.com From owner-diagnostics@net.bio.net Wed Sep 16 23:00:00 1998 Path: biosci!biosci!not-for-mail From: yatika kohli Newsgroups: bionet.diagnostics Subject: Re: DNA isolation from fungal spores Date: 17 Sep 1998 04:39:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tqsdn$5so@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Dr. O'Donnell, You might find these two references to be helpful; 1) Lee, S.B. and Taylor, J.W. 1990. Isolation of DNA from fungal mycelia and single spores. PCR protocols: A Guide to Methods and Applicaitons 282-287. 2) Kuske c.R. et al.,1998. Small-scale DNA sample preparation Method for field PCR detection of microbial cells and spores in soil. Appl Environ Microbiol vol 64 (7): 2463-2472. Hope this helps. Cheers! Yatika Kohli, Ph.D. Project Scientist Ontario Ministry of Health Etobicoke, ON Canada. >From biosci-request@net.bio.net Tue Sep 15 04:58:33 1998 >Received: by net.bio.net (8.9.1a/8.9.1) id EAA06782 > for diagnost-list; Tue, 15 Sep 1998 04:05:07 -0700 (PDT) >Received: by net.bio.net (8.9.1a/8.9.1) id EAA06768; > Tue, 15 Sep 1998 04:05:07 -0700 (PDT) >Message-Id: <199809151105.EAA06768@net.bio.net> >To: nobody@net.bio.net >X-Really-To: diagnost@net.bio.net >Newsgroups: bionet.diagnostics >From: "Kevin O'Donnell" >Organization: Scottish Agricultural Science Agency >Date: Tue, 15 Sep 1998 12:09:42 +0000 >Subject: DNA isolation from fungal spores > >Can anyone suggest a good (i.e. rapid, simple) method for isolating >DNA from fungal spores? The quality of DNA has only to be good enough for >PCR or LCR ampliciation, rather than cloning, orsequencing. > >Replies to the list please, rather than private email. > >Thanks, > >Kevin >Dr Kevin O'Donnell >Diagnostics and Molecular Biology >Scottish Agricultural Science Agency Tel 0131 244 8924 >Edinburgh Fax 0131 244 8926 >EH12 8NJ UK > ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From owner-diagnostics@net.bio.net Wed Sep 16 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Kybele Newsgroups: bionet.diagnostics Subject: Wanted : Glucose Monitoring Systems and Test Strips Date: 17 Sep 1998 02:58:49 -0700 Organization: Exodus Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6tqmgp$o1m@net.bio.net> NNTP-Posting-Host: net.bio.net We are importer and distributor of Glucose Monitoring Systems and Test Strips used in such devices. We would like to hear from appropriate wholesalers and manufacturers of the these items. Ana-Med Ltd. Resat Suthan From owner-diagnostics@net.bio.net Mon Sep 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Dave Jensen Newsgroups: bionet.diagnostics Subject: Thousands of Archived Posts about Bio-Careers Date: 22 Sep 1998 02:13:37 -0700 Organization: Search Masters International Lines: 20 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6u7po1$2h9@net.bio.net> NNTP-Posting-Host: net.bio.net If you are not yet familiar with the WWW site known as the Bio Online Career Discussion Forum, you ought to come over and take a look. This site, a part of the Bio Online career center, offers a huge selection of current and past posts about careers in Biotechnology and Pharmaceuticals. There are literally thousands of posts gathered over two years. The site address is http://www.bio.com/hr/forum/. You'll note that it is entirely non-commercial in nature. There are no annoying banner ads, no spam postings, no resume or job posts, etc. In other words, this site is for you to inquire with your peers or our group of Mentors about any issue you would like to discuss related to bio-employment. Customized newsgroup software allows for a very sophisticated retrieval process. See you online! Dave Jensen, Moderator http://www.bio.com/hr/forum/ From owner-diagnostics@net.bio.net Thu Sep 24 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Hamish Scott Newsgroups: bionet.diagnostics Subject: DNA extraction from stained histo slides Date: 25 Sep 1998 04:05:53 -0700 Organization: Division of Medical Genetics Lines: 29 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6ufteh$7j8@net.bio.net> NNTP-Posting-Host: net.bio.net Dear all, We have a liver needle biopsy that has been stained with ?? (H & E). It is vital to extract DNA from this to offer a diagnosis to a family. Does anyone have a reliable protocol for exteaction of PCRable DNA from something like this? All input appreciated. Yours Hamish -- ______________________________________________________ Dr Hamish S. Scott Division of Medical Genetics University of Geneva Medical School 1 rue Michel Servet 1211 Geneva 4 SWITZERLAND Ph: (+41 22) 702 5719 Fax: (+41 22) 702 5706 email: Hamish.Scott@medecine.unige.ch ______________________________________________________