From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Andre Cypreste de Miranda Newsgroups: bionet.diagnostics Subject: Doença rara Date: 12 Oct 1998 03:18:06 -0700 Organization: Urbi Network InterNetNews server Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsl0u$k21@net.bio.net> NNTP-Posting-Host: net.bio.net Sou cirurgiao buco-maxilo no RJ. Recentemente, um amigo meu adquiriu uma doença rara chamada SINDROME DE RETRAÇAO DE DUPUYTREN. A ocorrência da doença se dá no pênis. Agradeço se alguém puder ajudar no diagnóstico e tratamento da doença. JMMiranda From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Isla Browning Newsgroups: bionet.diagnostics Subject: Carlaviruses-infection of Nicotiana and Physalis spp. Date: 12 Oct 1998 02:22:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vshoc$b1a@net.bio.net> NNTP-Posting-Host: net.bio.net Do you know of any carlaviruses which systemically infect Nicotiana bigelovii, N. glutinosa, N. rustica, N. tabacum, or Physalis floridana? Please reply to newsgroup. Dr Colin Jeffries Scottish Agricultural Science Agency East Craigs Edinburgh. From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Krzysztof Wroblewski Newsgroups: bionet.diagnostics Subject: Fetal Ultrasound Date: 12 Oct 1998 02:34:08 -0700 Organization: University of Pennsylvania Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsieg$c26@net.bio.net> NNTP-Posting-Host: net.bio.net Fetal Echocardiography Homepage located at the URL http://www.med.upenn.edu/fetus contains the largest free library of fetal heart images. Over 100 high quality ultrasound images obtained using different ultrasound techniques: 2D-echo, Color Doppler, M-mode, and Pulse Doppler. The Homepage contains also a Discussion Forum. The goal of this forum is to provide a means for exchange of information about fetal echocardiography, cardiology and general fetal ultrasound. Feel free to post any question or problem related to this field. If you have any interesting case, please post an URL to the site where images can be viewed or send an e-mail to krzyszto@mail.med.upenn.edu to arrange for the images to be publicly available. From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Hart, Alan" Newsgroups: bionet.diagnostics Subject: Filters Date: 12 Oct 1998 03:50:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsmto$nni@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Everyone, I want to draw blood from a Vacutainer into a syringe so that what ends up in the barrel of the syringe is free of cells. Can anyone suggest a suitable filter to put in-line between the needle and the syringe to achieve this end? I appreciate that as filters are often uni-directional, I may have to turn the filter around. Thanks, Alan Hart From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jorg Kirberg Newsgroups: bionet.diagnostics Subject: Re: Filters Date: 12 Oct 1998 05:21:40 -0700 Organization: nki Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vss8k$662@net.bio.net> NNTP-Posting-Host: net.bio.net In article <6vsmto$nni@net.bio.net>, "Hart, Alan" wrote: > I want to draw blood from a Vacutainer into a syringe so that what ends > up in the barrel of the syringe is free of cells. Can anyone suggest a > suitable filter to put in-line between the needle and the syringe to > achieve this end? I would presume 'filtering-away' cells will result in clogging of your filter rather soon. Can't you centrifuge the vacutainer ? Just a thought, jorg --------------------------------------------------------------------- Correct E_mail address kirberg@nki.nl From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Jean-Francois Reveillard Newsgroups: bionet.diagnostics Subject: Medicine Net TV Date: 12 Oct 1998 04:02:56 -0700 Organization: Progress Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsnl0$p0o@net.bio.net> NNTP-Posting-Host: net.bio.net "The first french medical internet channel" Ouverture depuis le 1er Septembre 1998 de la première chaine internet dédiée à la médecine http://progress-tv.com Retrouvez tous les mois de nouveaux programmes, accès gratuit, démonstrations pratiques, débats, grace a la technologie de Realnetwork. Cordialement JF Réveillard (webmaster) From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Niall Byrne Newsgroups: bionet.diagnostics Subject: TB diagnosis - primates Date: 12 Oct 1998 03:58:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsnck$odr@net.bio.net> NNTP-Posting-Host: net.bio.net CSIRO and CSL have announced a test for human TB in primates. It's the latest in a series of gamma interferon-based tests for TB that was first developed for the Australian cattle industry (for M. bovis in cattle and other bovines), then for tuberculosis in humans. Their press release and information sheet are on CSIRO Animal Health's website at http://www.ah.csiro.au/newsline/press_rel/index.htm#1998 http://www.ah.csiro.au/TB%20infosheet.html _________________ Niall Byrne Science Communication Consultant E-mail: niallb@bigpond.com, web: users.bigpond.com/niallb From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: rhmorse Newsgroups: bionet.diagnostics Subject: Molecular Diagnostics Date: 12 Oct 1998 03:50:56 -0700 Organization: American Digital Network Lines: 7 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsmug$no0@net.bio.net> NNTP-Posting-Host: net.bio.net If there is an organization of laboratories which perform the diagnosis of genetic disorders (molecular genetics) within the pacific rim/Asia, such as CORN in the US or EDDNAL in Europe, would someone please send me the contact information. Thank you in advance. Randy Morse From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Oct 1998 03:27:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vslj6$l93@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Won-Jong Jang Newsgroups: bionet.diagnostics Subject: Please... ELISA background... Date: 12 Oct 1998 03:49:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsmrm$nir@net.bio.net> NNTP-Posting-Host: net.bio.net We are developing an antigen capture ELISA using monoclonal antibodies (Mab). The methods are here; 1. The first Mab (capture Ab; in carbonate buffer, pH 9.6) are added onto microtitre plates (Costar; Cat No. 3590). Incubated for overnight at 4oC. 2. The plates were blocked with 3% BSA (Sigma, A4503) in TBST for 2 h at 37oC. 3. The plates were washed three times and added diluted antigen solution and TBST (negative control). Incubated for 2 h at 37oC. 4. The plates were washed seven times and added biotinylated Mab (Detector Ab) in 0.1% BSA-PBST. Incubated for 2 h at 37oC. 5. The plates were washed seven times and added Streptavidin alkaline phosphatase conjugate (DAKO, D0396) solution (1:10,000 diluted in 0.1% BSA-PBST). Incubated for 30 min at 37oC. 6. The plates were washed seven times and added substrate solution. Incubated for 40 min at RT. 7. And added amplifier and incubated at RT for 10 min. 8. Stop the reaction and read the OD at 490 nm. We used cyclic amplification system of Self (J Imm Methods, 76;389) at the ELISA. This works fine except that we get a high background. Not only that, we get a high background if we just add negative control (TBST). So, what is happening is that the detector Mabs (biotinylated) are cross react to the capture Mabs coated on the wells. The capture and detector Mab are the same Mab purified using Protein A column from ascitic fluids. Does anyone have an explanation for this? Even better, can anyone suggest a way to overcome it? It is commonly stated that biotin has many binding sites for one antibody molecule and thus amplifies the reaction (i.e. if many avidin-En. are linked through one biotin to an antibody, it may lead signal amplification). In practice, is it possible? I would like to know the difference (signal noise ratio, sensitivity, etc.) between direct conjugation of enzyme to Ab and biotin-Ab through Streptavidin-enzyme system. Please respond to either this posting or e-mail any answers you might have directly to me at reattack@chollian.net Any help would be appreciated. Thanks, Jang Won-Jong Jang, Ph D. Department of Microbiology Seoul National University, College of Medicine 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, Korea Tel. (Lab.) 02) 740-8312 (Home) 02) 996-4551 Fax: 02) 743-0881 E-mail: reattack@chollian.net, reattack@plaza.snu.ac.kr From owner-diagnostics@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Nigel J.Osborn" Newsgroups: bionet.diagnostics Subject: Integration software for FPLC? Date: 12 Oct 1998 02:44:20 -0700 Organization: Amersham, Bucks Lines: 10 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <6vsj1k$cvk@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know of any integration software available for FPLC? Ideally I would like to be able to scan in a chromatogram and get it to tell me peak areas and relative peak areas. Preferably shareware though commercial products also would probably be O.K. Nigel Osborn. From owner-diagnostics@net.bio.net Sun Oct 18 23:00:00 1998 Path: biosci!biosci!not-for-mail From: ana Newsgroups: bionet.diagnostics Subject: HELP Date: 19 Oct 1998 01:33:03 -0700 Organization: Tel-Aviv University Computation Center Lines: 7 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <70etfv$kk5@net.bio.net> NNTP-Posting-Host: net.bio.net Please can enybody suggest where I can find information about the application of DNA probes in bacterial diagnostics (both clinical and food industry). Thanks in advance. Please reply on my e-mail: g5dima1@post.tau.ac.il From owner-diagnostics@net.bio.net Tue Oct 20 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell Newsgroups: bionet.diagnostics Subject: PCR- in-situ hybridisation Date: 21 Oct 1998 01:31:37 -0700 Organization: Scottish Agricultural Science Agency Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <70k659$d21@net.bio.net> NNTP-Posting-Host: net.bio.net Can anyone recommend some good papers on PCR-in situ hybrisisation? I seem to recall one from Biotechniques (I think) a couple of years ago but my copies are incomplete. If anyone can supply the Biotechniques reference in particular, I'd be very grateful. Kevin Dr Kevin O'Donnell Diagnostics and Molecular Biology Scottish Agricultural Science Agency Tel 0131 244 8924 Edinburgh Fax 0131 244 8926 EH12 8NJ UK From owner-diagnostics@net.bio.net Wed Oct 21 23:00:00 1998 Path: biosci!biosci!not-for-mail From: stewartl@best.com Newsgroups: bionet.diagnostics Subject: Re: PCR- in-situ hybridisation Date: 22 Oct 1998 04:50:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <70n65e$pc9@net.bio.net> NNTP-Posting-Host: net.bio.net Kevin O'Donnell wrote: > > Can anyone recommend some good papers on PCR-in situ hybrisisation? I > seem to recall one from Biotechniques (I think) a couple of years ago but my > copies are incomplete. If anyone can supply the Biotechniques > reference in particular, I'd be very grateful. There are a number of PCR in situ online links at: http://www.alkami.com/refmthd.htm#insitu In addition, there are other pages at this site, http://www.alkami.com, that have PCR troubleshooting tips and a comprehensive collection of other PCR related information (I think it's better than the PCR Jump Station). BioTechniques has a web site where you could probably track down the article you're looking for at: http://www.BioTechniques.com/ However, my experience with thier article search program has been spotty. Good luck. From owner-diagnostics@net.bio.net Thu Oct 22 23:00:00 1998 Path: biosci!biosci!not-for-mail From: DADT2243 Newsgroups: bionet.diagnostics Subject: hepatitis B Sub-typing Date: 23 Oct 1998 09:18:41 -0700 Organization: AOL http://www.aol.com Lines: 2 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <70qa91$8do@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a lab that can do hepatitis B sub typing for AY/AD. Does anyone know of one? From owner-diagnostics@net.bio.net Sun Oct 25 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Dr.Narayana R.Isola" Newsgroups: bionet.diagnostics Subject: Amplitype filters Date: 26 Oct 1998 01:11:42 -0800 Organization: Oak Ridge National Laboratory Lines: 18 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <711ece$jsk@net.bio.net> NNTP-Posting-Host: net.bio.net Any one using HLA detection kits or or Amplitype PM amplification kits from Perkin-Elmer. I would like to use the used filters and check see if I could detect the hybridized DNA by mass spectrometry. Guess I am too cheap and lazy to buy the kit and do it myself (lol). As a quick and dirty method I would like to have the used filters. Please contact me Narayana R.Isola Ph.D., Oak Ridge National Laboratories, Tel 423 574 5893 Fax 423 576 2115 E-mail isolan@ornl.gov From owner-diagnostics@net.bio.net Tue Oct 27 22:00:00 1998 Path: biosci!biosci!not-for-mail From: allergie Newsgroups: bionet.diagnostics Subject: grapes juice Date: 28 Oct 1998 08:27:56 -0800 Organization: Wanadoo - (Client of French Internet Provider) Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <717gmc$iqu@net.bio.net> NNTP-Posting-Host: net.bio.net Does anybody knows about the microbiology legislation needed for grapes juice analyses in the european countries, french country or others countries? If you any experienc about it please contact me at: cabee@wanadoo.fr thanks -- philippe