From owner-diagnostics@net.bio.net Tue Mar 02 22:00:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Mar 1999 02:09:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7bj1og$nu0@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Wed Mar 03 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Peter Ahnert Newsgroups: bionet.diagnostics Subject: Detection of Parodontosis and Periodontosis Date: 4 Mar 1999 01:30:50 -0800 Organization: Ohio State University Lines: 26 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7bljsa$8k7@net.bio.net> NNTP-Posting-Host: net.bio.net Hi, I would like to find out how parodontosis or periodontosis are detected these days. I am especially interested in microbilogical methods. What do you do if you suspect your patient has parodontosis/periodontosis? Do you send samples to a laboratory, do you purchase a kit to do it yourself? How early can you detect the disease with the test you are using? Can you monitor the progress of treatment? Please let me know any relevant information. I also would not mind if you fax material about your tests to 614-292-6773 :) I also appreciate any pointers to sources of relevant information. Thanks a lot, Peter. ====================================================== Peter Ahnert Department of Biochemistry - The Ohio State University 484 W. 12th Ave. # 776, Columbus, OH 43210, USA Tel.: (614) 292-3319 Fax.: (614) 292-6773 E-mail: Ahnert.1@osu.edu ====================================================== From owner-diagnostics@net.bio.net Tue Mar 16 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Beat Ludin Newsgroups: bionet.diagnostics Subject: Live Microscopy Discussions Date: 17 Mar 1999 01:54:10 -0800 Organization: Life Imaging Services Lines: 32 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7cnu42$13u@net.bio.net> NNTP-Posting-Host: net.bio.net Dear list members I would like to draw your attention to a newly opened forum at http://www.lis.ch. The discussion forum features topics pertaining to the whole area of live microscopy (i.e. microscopy on living specimen) specifically. As this is new, the place may look rather deserted right now. But I hope we will soon have a lively discussing community there. So if you have a question or a topic you always wanted to discuss, don't hesitate to post it. BTW, in order to keep spam out, all of the groups require email-verified registration (free). There are also some special interest groups that are only accessible to members. See you there, Beat If you would like to found (and moderate) a new special interest group, e.g. to discuss specific software or hardware on neutral ground, send your application to forums@lis.ch. LIFE IMAGING SERVICES - visit our new web site at www.lis.ch +----------------------------------------------------------- | Dr. B. Ludin | Life Imaging Services fon ++41 (0)79 235 7154 | Muehletalweg 22 fax ++41 (0)86 062 296 3160 NEW! | CH-4600 Olten beat.ludin@lis.ch | Switzerland http://www.lis.ch From owner-diagnostics@net.bio.net Tue Mar 16 22:00:00 1999 Path: biosci!biosci!not-for-mail From: BLangrand@aol.com Newsgroups: bionet.diagnostics Subject: Coenzyme A measurement Date: 17 Mar 1999 01:54:22 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7cnu4e$148@net.bio.net> NNTP-Posting-Host: net.bio.net I am searching for someone who has already measured CoA content in liver tissue using the method described by Bergmeyer. Thanks in advance. Carole From owner-diagnostics@net.bio.net Wed Mar 17 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Nomads Newsgroups: bionet.diagnostics Subject: Diabetes Diagnostic Systems Required Date: 18 Mar 1999 06:35:29 -0800 Organization: Superonline Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7cr2vh$7nq@net.bio.net> NNTP-Posting-Host: net.bio.net Glucometers and Elite Test Strips required in bulk. Nomads http://members.xoom.com/busicom From owner-diagnostics@net.bio.net Thu Mar 18 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Innovatek Newsgroups: bionet.diagnostics Subject: Re: Diabetes Diagnostic Systems Required Date: 19 Mar 1999 08:48:53 -0800 Organization: Genix Lines: 20 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7ctv5l$qq3@net.bio.net> NNTP-Posting-Host: net.bio.net Would you be interested in ONE TOUCH strips in bulk? Brian ====================== Nomads wrote: > Glucometers and Elite Test Strips required in bulk. > > Nomads > > http://members.xoom.com/busicom -- ========================================= Please remove nospam from e-mail address before replying ========================================= From owner-diagnostics@net.bio.net Thu Mar 18 22:00:00 1999 Path: biosci!biosci!not-for-mail From: kevin gilbride Newsgroups: bionet.diagnostics Subject: South of Boston Date: 19 Mar 1999 01:02:02 -0800 Organization: b.u. Lines: 4 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7ct3qa$htr@net.bio.net> NNTP-Posting-Host: net.bio.net Bench scientist with over nine years of protein biochemistry and molecular biology experience seeks ground floor oportunity with genomics or diagnostic lab south of Boston. For more information email gilbride@bu.edu From owner-diagnostics@net.bio.net Sun Mar 21 22:00:00 1999 Path: biosci!biosci!not-for-mail From: Stuart Harbron Newsgroups: bionet.diagnostics Subject: Diagnostic Insight Database Date: 22 Mar 1999 08:18:39 -0800 Organization: The Enzyme Technology Consultancy Lines: 25 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7d5qgv$p01@net.bio.net> NNTP-Posting-Host: net.bio.net To: Dr Kevin O'Donnell From: Dr Stuart Harbron (s.harbron@etc.uk.com) Subject: Diagnostic Insight Database We have made improvements to the Diagnostic Insight Database. The Database now links to the IBM Intellectual Property Site and to PubMed. This means that you can get full bibliographic information from your search results from the Diagnostic Insight Database. This powerful combination puts even more information at your fingertips. Take a look at: http://www.etc.uk.com/cgi-bin/dbman/db.cgi ============================================================ Stuart Harbron, PhD Principal Consultant The Enzyme Technology Consultancy Tel: (+44) 1442 385740 Fax: (+44) 1442 384084 mailto:s.harbron@experts.co.uk http://www.experts.co.uk From owner-diagnostics@net.bio.net Wed Mar 24 22:00:00 1999 Path: biosci!biosci!not-for-mail From: "Patrick C. Brunner" Newsgroups: bionet.diagnostics Subject: glue-traps and PCR Date: 25 Mar 1999 09:22:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7ddrch$sol@net.bio.net> NNTP-Posting-Host: net.bio.net Dear colleagues We use PCR-RFLP of mtDNA to successfully distinguish several thrips (= "tiny insects") species. Our protocols work well with fresh, frozen or dry specimens. However, some have been caught and stored on commercially available glue traps (,tanglefoot" ?), and we have a hard time to get a reasonable PCR product from these DNA extractions. We suspect that the glue on the traps inhibits the PCR reaction. We tried to remove the glue mechanically and with benzine, with little success. Diluting the DNA is problematic too, because exctractions often yield not more then pico-grams / ul. Has anyone similar experiences and a solution for this problem? Any input is highly appreciated! Sincerely, Patrick Brunner ---------------------Dr. Patrick C. Brunner------------------ Swiss Federal Research Station Schloss, Lab. 4 phone: +41 (1) 783 6331 CH-8820 Waedenswil fax: +41 (1) 783 6434 Switzerland e-mail: patrick.brunner@faw.admin.ch -------------------------------------------------------------- From owner-diagnostics@net.bio.net Sun Mar 28 23:00:00 1999 Path: biosci!biosci!not-for-mail From: Russell Farris Newsgroups: bionet.diagnostics Subject: False negatives in tests for Chalmydia pneumoniae Date: 29 Mar 1999 00:46:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7dnel5$4l1@net.bio.net> NNTP-Posting-Host: net.bio.net I have found hints that antibiotics, NSAIDS, and perhaps other things will cause false negatives in the tests for bacterial infections, but I haven't been able to find very much on this problem. I am particularly interested in Chlamydia pneumoniae tests, but would be glad to receive anything on the subject. Thanks for your attention. Russ Farris From owner-diagnostics@net.bio.net Sun Mar 28 23:00:00 1999 Path: biosci!biosci!not-for-mail From: biohazard1999@my-dejanews.com Newsgroups: bionet.diagnostics Subject: Applications and Methods and http://come.to/biomedpage Date: 29 Mar 1999 00:57:54 -0800 Organization: Deja News - The Leader in Internet Discussion Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7dnfai$bp0@net.bio.net> NNTP-Posting-Host: net.bio.net Are you looking for good scientific information in diagnostics, biomedicine and biology ? Laboratory protocols, method applications, FREE medline, C.elegans, yeast two-hybrid systems, biomedicine, genome projects, databases. You will find all answers at BIOMEDPAGE: http://come.to/biomedpage Please visit my BIOMEDPAGE ! Thank you in advance. -----------== Posted via Deja News, The Discussion Network ==---------- http://www.dejanews.com/ Search, Read, Discuss, or Start Your Own From owner-diagnostics@net.bio.net Mon Mar 29 23:00:00 1999 Path: biosci!biosci!not-for-mail From: Robert DiSilvestro Newsgroups: bionet.diagnostics Subject: Isoprostane ELISAs Date: 30 Mar 1999 01:05:21 -0800 Organization: The Ohio State University Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7dq44h$ad8@net.bio.net> NNTP-Posting-Host: net.bio.net Has anyone used the ELISA kits for isoprostanes? I have some doubts about specificity, but a bigger concern is just performance. The kit I have used from Assay Designs worked well at first, but has been very poor in the last year (ie bad spike and recovery, high and variable values for control subjects, and poor agreement between duplicates). Anybody have any comments? NOTE: my real E-mail address does not contain Xs.