From owner-diagnostics@net.bio.net Tue May 04 09:26:00 1999 Path: biosci!biosci!not-for-mail From: Gottfried Griesmayr Newsgroups: bionet.diagnostics Subject: correct mean of replicates Date: 4 May 1999 03:26:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmi15$11e@net.bio.net> NNTP-Posting-Host: net.bio.net How to calculate correctly the mean of replicates in an immunoassay? We are used to calculate first the mean of the measurement values of all replicates of a sample (e.g. mean of OD measured with the ELISA reader), then we calculate the concentration of this sample based on the mean (e.g. using a standard curve). The concentrations of individual replicates are reported in addition. Others calculate concentrations only for the individual replicates and report the mean of replicate concentrations to be the sample concentration. This two ways end up with identical results, as long as the concentration axis and calibration curves are linear and there is a linear relation between measurement values and concentration. But it is obviously not the same when we are dealing with a logarithmic concentration axis! If we take simply the arithmetic mean of individually calculated replicates in such case, the higher replicates would be weighted more than the lower replicates. An outlier with high reading would influence the result much more than an outlier with low reading. For eliminating this effect we would have to take the geometric mean (multiply n replicate values and draw the n-th root of the product). At least for mathematical reasons this would be the only correct solution in my opinion, but I have heard that nobody takes care on this matter. I was told that it is a convention or at least general practice, to use the arithmetic mean of replicate concentrations without respect to the axis format! Is this really true? Your comments are appreciated. Gottfried Griesmayr E-mail: gottfried.griesmayr@anthos-labtec.com Homepage: http://www.anthos-labtec.com From owner-diagnostics@net.bio.net Tue May 04 09:27:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 4 May 1999 03:27:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmi30$12n@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Tue May 04 09:43:00 1999 Path: biosci!biosci!not-for-mail From: Andrew M Smith Newsgroups: bionet.diagnostics Subject: This Week's Chemistry & Life Science Industry Headlines Date: 4 May 1999 03:43:19 -0700 Organization: selectscience Lines: 130 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmj07$21d@net.bio.net> NNTP-Posting-Host: net.bio.net This week, www.selectscience.net reported on 50 news stories the day they happened, so if you need to keep up to speed on the latest business developments in chemistry and life science, make the news ticker on www.selectscience.net part of your daily routine. Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net This week's headlines were: La Jolla Pharmaceutical Scientists Tolerize B Cells That Thwart Xenotransplantation FDA Approves First Home Test for Hepatitis C Virus Metra Biosystems Reports Third Quarter Results PE Biosystems Reports Sharply Higher Earnings And Celera Genomics Makes Progress In Establishing Operations Thermo Electron and Thermo Power Considering Merger Proposal Pharmacia & Upjohn Reports First-Quarter Sales And Earnings Targeted Genetics Reports First Quarter 1999 Financial Results Genome Therapeutics Strengthens Business Development Team Aurora Biosciences Reports First Quarter 1999 Financial Results Dade Behring and HemoSense Agree to Collaborate On New-Generation Point-of-Care and Home Coagulation Tests Hyseq Announces First Quarter Financial Results STERIS Reports Record Fiscal Fourth Quarter and Full Year Results Perkin-Elmer Shareholders Approve Recapitalization Proposal Maxygen Obtains Exclusive License to Caltech 'StEP' Directed Evolution Technology Steris CFO Resigns Bio-Rad Reports Results for First Quarter 1999 PPL Therapeutics plc Preliminary Results European Union to Adopt Common Framework for Electronic Document Signature Becton Dickinson Announces Planned Acquisition of Clontech Laboratories Trinity Biotech Announces Record First Quarter Profits; Net Profit up 100% with 65% Growth in Earnings Per Share Caliper Technologies CorpLaunches New Manufacturing Facility and Corporate Headquarters BIO Strongly Backs U.SSenBennett's Efforts To Protect Patient Confidentiality Gel Documentation System Wins UK Design Council Millenium Product Stats Aronex Pharmaceuticals, IncReports First Quarter 1999 Results Delsys Pharmaceutical Names Marty Kashef, Ph.D., Director of Engineering Sciences Goats Cloned by Tufts University School of Veterinary Medicine and Genzyme Transgenics Corp Radiation Missing Cell Nucleus May Still Cause DNA Changes GlaxoWellcome Wins Queens Award for Technological Achievement Megabios Acquires Angiogenesis Gene Pharmacyclics Completes Enrollment in Phase I ANTRIN(TM) Trial For Atherosclerosis Beckman Coulter Announces First Quarter 1999 Results Sarnoff & Mediscience Commercialize Light-Based Molecular Technology For Cervical Cancer Caliper Files Suit Against Aclara for Trade Secret Violations Sequenom Obtains Patents for DNA MassArray Technology Aclara Biosciences & PE Biosystems Sign Drug Screening Collaboration Millipore Reports First Quarter Results CombiChem Appoints Michael GRusnak to the Position of Vice President, Business Development TRANSGENE Announces First Quarter Results Bailey to Head Health Industry Manufacturers Association (HIMA) AmeriPath, IncAnnounces 1999 First Quarter Results Coulter Pharmaceutical Reports 1999 First Quarter Financial Results Abbott Announces Executive Promotions Genset Reports First Quarter 1999 Financial Results Genset Reinforces Its Obesity Program with the Creation of a New Physiological Genomics Department and Publishes First Gene Discovery Results Mettler-Toledo International IncReports First Quarter 1999 Results Rohm and Haas Withdraws Tender Offer; Will Acquire Morton through Merger Progenics Report on Novel HIV Inhibitor is Published in The Journal Of Virology Centaur Pharmaceuticals Announces First Quarter 1999 Financial Results EG&G Announces 24% Increase In Diluted Earnings Per Share For The First Quarter 1999 Human Genome Sciences Reports First Quarter Financial Results From owner-diagnostics@net.bio.net Tue May 04 09:52:00 1999 Path: biosci!biosci!not-for-mail From: Andrew M Smith Newsgroups: bionet.diagnostics Subject: Now, when was that conference? Date: 4 May 1999 03:52:46 -0700 Organization: selectscience Lines: 19 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmjhu$343@net.bio.net> NNTP-Posting-Host: net.bio.net At www.selectscience.net we have details on over 400 conferences, symposia, exhibitions, short courses and lectures, and that number is growing every day. If you want to find out about upcoming events in your discipline, come to www.selectscience.net If you are organising a meeting, please drop me a line (andrew@selectscience.net) so I can add your event to our database. I'm looking forward to hearing from you, Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net From owner-diagnostics@net.bio.net Tue May 04 09:56:00 1999 Path: biosci!biosci!not-for-mail From: "BIOMEDIA (S. Bobichon)" Newsgroups: bionet.diagnostics Subject: Options for the Control of Influenza iV Date: 4 May 1999 03:56:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmjpj$3lm@net.bio.net> NNTP-Posting-Host: net.bio.net We are delighted to announce that the FOURTH INTERNATIONAL CONFERERENCE IN THE SERIES 'OPTIONS FOR THE CONTROL OF INFLUENZA' WILL BE HELD IN CRETE (GREECE) FROM 23 To 28 SEPTEMBER 2000. This meeting will address many aspects of the field of influenza and will provide a unique opportunity to review achievements of the past century and to prepare for future challenges. If you wish to the 1st announce, please send us an Email with your name and postal address to options4@wanadoo.fr. Sincerely yours, Options IV Conference Secretariat BIOMEDIA 101 rue Mademoiselle - F-75015 Paris From owner-diagnostics@net.bio.net Tue May 04 10:13:00 1999 Path: biosci!biosci!not-for-mail From: Andrew M Smith Newsgroups: bionet.diagnostics Subject: Searchable laboratory vacancy database Date: 4 May 1999 04:13:21 -0700 Organization: selectscience Lines: 12 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmkoh$59l@net.bio.net> NNTP-Posting-Host: net.bio.net At www.selectscience.net we have a fully searchable database of nearly 300 current job vacancies in the applied chemistry and life science sector, and that number is growing every day. If you are looking for the perfect new job, come to www.selectscience.net If you have a vacancy, you can add it to the www.selectscience.net database FREE OF CHARGE, so come to the site now. I'm looking forward to your visit, Andrew Smith Editor, www.selectscience.net From owner-diagnostics@net.bio.net Tue May 04 10:51:00 1999 Path: biosci!biosci!not-for-mail From: Joan Boyce Newsgroups: bionet.diagnostics Subject: 1999 Source Book Available Free to Researchers Date: 4 May 1999 04:51:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmn0n$ae7@net.bio.net> NNTP-Posting-Host: net.bio.net Obtain a free copy of the 1999 Source Book, a comprehensive guide to bioresearch products, by filling in the request form at the BioSupplyNet site: http://www.biosupplynet.com. The Source Book provides up-to-date information about reagents, lab instrumentation, antibodies, custom services and supplies. Biomedical researchers will find that the unique organization of the Source Book simplifies product searching and speeds the gathering of comparative information. The Source Book indexes products in 2650 categories, and provides up-to-date contact information, including WWW and e-mail addresses, for 3500 suppliers. Bench Basics comparative tables allow apples-to-apples comparisons of products. A special Lab Set-up Check List simplifies the process of putting together a new laboratory. Order your free copy at The BioSupplyNet web site at http://www.biosupplynet.com From owner-diagnostics@net.bio.net Tue May 04 10:54:00 1999 Path: biosci!biosci!not-for-mail From: Erling Sandsdalen Newsgroups: bionet.diagnostics Subject: Error message, Microplate Spectrophotometer, SPECTRAmax PLUS Date: 4 May 1999 04:54:20 -0700 Organization: Fiskeriforskning Lines: 11 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gmn5c$aj4@net.bio.net> NNTP-Posting-Host: net.bio.net Hello ! When trying to start our microplate reader, we get the following error message: "Fatal error 315". Does anybody out there know what this means? Thank you in advance !!! Best regards Erling Sandsdalen From owner-diagnostics@net.bio.net Fri May 07 14:59:00 1999 Path: biosci!biosci!not-for-mail From: SelectScience Newsgroups: bionet.diagnostics Subject: This Week's Chemistry & Life Science Industry Headlines Date: 7 May 1999 08:59:52 -0700 Organization: SelectScience Lines: 99 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7gv2lo$kuf@net.bio.net> NNTP-Posting-Host: net.bio.net This week, www.selectscience.net reported on 37 news stories the day they happened, so if you need to keep up to speed on the latest business developments in chemistry and life science, make the news ticker on www.selectscience.net part of your daily routine. Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net This week's headlines were: DNA Chip Technology Enhances Consumer Safety and Water Quality Celera Genomics Commences Sequencing the Drosophila Melanogaster Genome Glaxo Wellcome, Emory University & Triangle Pharmaceuticals Resolve Coviracil Dispute Advanced Tissue Sciences Presents Data on Vascular Endothelialization Growth Factor (VEGF) Secretion CYTOGEN Reports First Profitable Quarter and Announces Strategic Emphasis on Prostate Disease Ribozyme Pharmaceuticals, Inc. Reports First Quarter Financial Results UCSB Researchers Disarm ""Trojan Horse"" Pathogens" Incyte Score Latest Victory in Patent Battle PE Biosystems Partners With Verdot Industries Bio-Rad Laboratories and Saigene Corporation enter into a worldwide exclusive licensing agreement Bio-Rad Reports Results For First Quarter 1999 Thermedics Detection Announces First Quarter Results Study examined safety of HFA-134a BDP compared to traditional Becolmethasone inhalers La Jolla Pharmaceutical Company Reports First Quarter 1999 Financial Results Genset and Johns Hopkins Sign Schizophernia Gene Agreement Form of Chinese Herb Found to Temper Immune System and Kill Cancer Cells Astra Files Suit Against German Generic Company What Changes is HCFA Proposing to the Medicare Coverage Process Nycomed Pharma Sold to Nordic Capital Progenics Pharmaceuticals, Inc. Reports First Quarter 1999 Results Thermo Electron To Buy Its Own And Its Subsidiaries' Securities SIBIA Neurosciences, Inc. Names Carla M. Suto, Ph.D. Vice President, HTS and Technology Development HemaSure Inc. Completes $9 Million Private Placement COBE Laboratories Trega Biosciences Reports First-Quarter Results; Revenues Increase 75% BD Labware & Kimble/Kontes Form Collaborative Agreement Roslin Institute & Geron Sign Major Deal to Advance Dolly Technology Affymetrix Reports First Quarter Results Dade Behring Announces Hoechst AG Will Increase Ownership Position QIAGEN and EVOTEC Form Joint Venture for the Development and Commercialization of High-Throughput Systems HemaSure Inc. and COBE BCT Inc. Initiate Patent Lawsuit With Pall Corporation EG&G/PE Analytical Instruments Deal on Schedule; US & German Rebiew Periods Expire New Genetic Study Points Way For Vaccine Reaction Research QIAGEN Up 33% in First Quarter Financial Results Inspire Awarded U.S. Key Patent for New Chemical Entities Cadus Pharmaceutial Corporation to Explore Strategic Alternatives Glyko, Inc. Acquires Biochemical Reagent Business from Oxford GlycoSciences Plc Sanofi Reports 6.8% Sales Growth in the First Quarter of 1999 (8.6 % for Pharmaceuticals) From owner-diagnostics@net.bio.net Mon May 10 12:47:00 1999 Path: biosci!biosci!not-for-mail From: "L. Stewart" Newsgroups: bionet.diagnostics Subject: Online PCR Resources Date: 10 May 1999 06:47:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7h6o27$sb6@net.bio.net> NNTP-Posting-Host: net.bio.net We'd like to remind you of one of the most comprehensive PCR resources on the Internet: http://www.alkami.com. Alkami's PCR resources include * Primer design tips - http://www.alkami.com/refprmr.htm * Primer design tools - http://www.alkami.com/refdsgn.htm * PCR protocol references - http://www.alkami.com/pgtopics.htm * PCR troubleshooting guide - http://www.alkami.com/reftrbl.htm * BioScience employment resources - http://www.alkami.com/pgjobs.htm * Monthly Concise PCR Reviews - http://www.alkami.com/pgreview.htm Featured for the month of May are: - PCR Enhancers and Inhibitors by Dr. Laura Ruth http://www.alkami.com/reviews/rvwmay99.htm - Online Primer Design: Issue 2 by Lance Larka http://www.alkami.com/reviews/rvwdsgn2.htm * FREE laboratory supply samples - http://www.alkami.com/pgorder.htm To subscribe to Alkami PCR Announcements please send email to subscribe@alkami.com To unsubscribe to Alkami PCR Announcements please send email to unsubscribe@alkami.com ALKAMI BIOSYSTEMS, Inc. Simplified Solutions for Complex Questions (tm) info@alkami.com http://www.alkami.com P.O. Box 11216 Berkeley CA 94712 USA From owner-diagnostics@net.bio.net Wed May 12 10:19:00 1999 Path: biosci!biosci!not-for-mail From: "L. Stewart" Newsgroups: bionet.diagnostics Subject: Online PCR Resources Date: 12 May 1999 04:19:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7hbo4g$rgl@net.bio.net> NNTP-Posting-Host: net.bio.net We'd like to remind you of one of the most comprehensive PCR resources on the Internet: http://www.alkami.com. Alkami's PCR resources include * Primer design tips - http://www.alkami.com/refprmr.htm * Primer design tools - http://www.alkami.com/refdsgn.htm * PCR protocol references - http://www.alkami.com/pgtopics.htm * PCR troubleshooting guide - http://www.alkami.com/reftrbl.htm * BioScience employment resources - http://www.alkami.com/pgjobs.htm * Monthly Concise PCR Reviews - http://www.alkami.com/pgreview.htm Featured for the month of May are: - PCR Enhancers and Inhibitors by Dr. Laura Ruth http://www.alkami.com/reviews/rvwmay99.htm - Online Primer Design: Issue 2 by Lance Larka http://www.alkami.com/reviews/rvwdsgn2.htm * FREE laboratory supply samples - http://www.alkami.com/pgorder.htm To subscribe to Alkami PCR Announcements please send email to subscribe@alkami.com To unsubscribe to Alkami PCR Announcements please send email to unsubscribe@alkami.com ALKAMI BIOSYSTEMS, Inc. Simplified Solutions for Complex Questions (tm) info@alkami.com http://www.alkami.com P.O. Box 11216 Berkeley CA 94712 USA From owner-diagnostics@net.bio.net Mon May 17 14:38:00 1999 Path: biosci!biosci!not-for-mail From: mail1@irix.xamu.edu.cn Newsgroups: bionet.diagnostics Subject: SoS:suspect a case of Creutafeldt-Jakob syndrome in china Date: 17 May 1999 08:38:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7hpd5h$kge@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Sir: I'm a neurologist,I need your help! I met a strange patient who was suspected Creutzfeldt-Jakob syndrome.But 14-3-3 beta protein in CSF was negative.What's the diagnosis on earth? any ideas? How to treat the patient?If you have answer ,You can send E-mail to lidan@irix.xamu.edu.cn. The case abstract is as follow: 48 YO Chinese F presant ,who hospitalized with the CC: progressive amnesia and stupidity for 3 Mons, unconsciousness and limbs tic for 4 days. 3 Mons prior to admission ,she was becoming forgetful,less-effective in house-working, spontaneous nonsense,eating without feeling hungry or full and defecation ,urination everywhere. There was no clear reason. 4 days prior to admission ,she was unconscious ,extremities tic ,myoclonus seizure, excretion complete incontinences. No fever ,no headache and no vomitting during all the course of disease. She was initially treated as the diagnosis of mental disturbance, but it didn't work. She was in her USOGH(usual state of good health). No vaccination ,trauma,toxication history and no special family/social history. PE: Vital Sign: stable CV, Pulm, Abd and Ext examination is N.A.D (no abnormal detected) Neurology System : Superficial coma state, primitive defence reflexes could be seen. Bilateral pupils were in same size,D m,showed a sensitive light reflex. Doll eye sign :(+) . Neck: soft Motor: Automatical movement and myoclonus seizure could be seen after stimulation. Extremities muscular hypertonia ,tendon hyporeflexia. Babinski's sign (-),other pathologic reflex: (-). Meningeal irritation sign (-). Sensory and co-ordination :can not be checked because of inability to co-operate. Lab: CBC: WBC 7.8¡A10^6/L,N:0.65,L:0.34 CSF: protein 400mg/L, Sugar 150mg/L Cl 126mmol/L Total cell count: 25¡A10^6 /L ;WBC 0¡A10^6/L Viral series: CMV; HSV ¢ñ/¢ò;Toxoplasma;Rubella IgM and IgG are all negative. 14-3-3 ¦A protein: (-) TB-DNA: (-) EEG: progressively increased periodical slow-apex and slow-spinal waves on the background of diffused slow wave. Brain biopsy: brain tissue mild congestion,focal lymph cell infiltration around small vessels, neuroglia mild hyperplasia ,neuron cells has no obvious alteration. Cranial CT and MRI :(-) Electrolytes ,liver and kidney function: All are normal. Up to now,the patient is being coma for near 2 Mons. She was not getting worse or better ,even though many heteropathies had been taken. Diagnosis? Therapy? Any ideas? From owner-diagnostics@net.bio.net Fri May 21 07:25:00 1999 Path: biosci!biosci!not-for-mail From: Russell Farris Newsgroups: bionet.diagnostics Subject: What happened to this newsgroup? Date: 21 May 1999 01:24:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7i358g$nba@net.bio.net> NNTP-Posting-Host: net.bio.net This was a very active group a few months ago. What happened? Russ Farris From owner-diagnostics@net.bio.net Mon May 24 13:50:00 1999 Path: biosci!biosci!not-for-mail From: SelectScience Newsgroups: bionet.diagnostics Subject: Last Week's Chemistry & Life Science News Headlines Date: 24 May 1999 07:50:42 -0700 Organization: SelectScience Lines: 86 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7ibp02$l9s@net.bio.net> NNTP-Posting-Host: net.bio.net Last week, www.selectscience.net reported on 31 news stories the day they happened, so if you need to keep up to speed on the latest business developments in chemistry and life science, make the news ticker on www.selectscience.net part of your daily routine. Andrew Smith (andrew@selectscience.net) Editor, www.selectscience.net Last week's headlines were: Peptide Therapeutics Appoints New Chairman Cantab Pharmaceuticals Reports First Quarter Financial Results and Significant Progress in Key Development Programs CuraGen Announces Completion of High Resolution Rat Genome Maps QIAGEN Announces 2-For-1 Stock Split and Conversion of Currency of its Par Value to EURO CLONTECH Receives ISO 9000 Certification Molecular Devices announces the Acquisition of Skatron Instruments AS Lonza Biologics and Genzyme Transgenics Corporation to Establish Strategic Alliance for Biopharmaceutical Production Millipore Acquires Bioprocessing Corp. QIAGEN Joins Valentis-DS Biologics Alliance for Manufacturing and Sales of Plasmid DNA Genmab Obtains Rights to IL15 Antibody Patents Roche Diagnostics Takes License Under Organon Teknika's Nucleic Acid Extraction Technology Vysis Reports Positive Q1 1999 Results Hemagen Announces 34% Increase in Sales From Same Quarter Last Year Protein Design Labs Announces Presentations of Clinical Results Using SMART Anti-CD3 Antibody Biogen Names Jennifer Jackson, Ph.D., as Director, Regulatory Affairs Life Technologies Completes Acquisition of Process Chromatography and Research Products Businesses from BioSepra IntegrLifeSciences Reports Results From 1999 Annual Meeting of Stockholders Steris Corporation to Acquire Assets of Ricerca, Inc. Biogen Names Michael Gilman, Ph.D., as Research Director of Molecular Biology ThermoQuest Announces First Quarter Results Thermo BioAnalysis Announces First Quarter Results Thermo Instrument Systems Announces First Quarter Results Thermo Optek Announces First Quarter Results Perkin-Elmer Sells Its Environmental And Process Analysis Division To SICK AG PE Corporation Declares Dividend Cerep SA Announces Drug Discovery Partnership with Pfizer New Scientific Advisory Board at Xenova Ontogen and Oxford Asymmetry International (OAI) Announce Collaboration Centocor Announces Senior Executive Appointment and Promotions Sale of Nycomed Pharma Completed Trinity Biotech Responds to Centocor Lawsuit From owner-diagnostics@net.bio.net Wed May 26 12:02:00 1999 Path: biosci!biosci!not-for-mail From: drand57@my-dejanews.com Newsgroups: bionet.diagnostics Subject: need help and information Date: 26 May 1999 06:02:44 -0700 Organization: Deja.com - Share what you know. Learn what you don't. Lines: 22 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7igrdk$1gr@net.bio.net> NNTP-Posting-Host: net.bio.net Hello! Can anybody help with the information (www,email adr. tel.,addr.) of medical organizations specialized in diagnostics,treatment,etc of STD (Trichomonus vaginalis specialists need). A friend of mine,doctor and B.of Sc. will soon going to US to meet with specialists of such area.She is very intrested of unformal meetings with collegues.To discuss their problems ,etc.May be anybody knows Taylor-Robinson works in such area,please help to her to contact with him or with other specialists Please help scientists to find each other and to meet. The first place of visit will be Washington. Please help to my friend.She is from Ukraine and speaks English a little. Contact email drand57@my-dejanews.com drand57@my-deja.com or direct to real helpers AndyPC@online.alkar.net --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.---