From owner-diagnostics@net.bio.net Tue Aug 03 08:09:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator [mailto:biohelp@net.bio.net] Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Aug 1999 02:08:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7o6bj3$g81@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Fri Aug 13 14:28:00 1999 Path: biosci!biosci!not-for-mail From: anatomy@ivtusa.com [mailto:anatomy@ivtusa.com] Newsgroups: bionet.diagnostics Subject: Human Anatomy Review Date: 13 Aug 1999 08:28:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7p1dit$rps@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, Enersoft Educational Services has produced a comprehensive study material on Human Anatomy, specifically designed for Medical students seeking bright success in examinations. The material is also good for researchers, professionals and anyone looking for information on Human Anatomy. More than 2000 medical personnel around the world have benefited from Human Anatomy Review. Please click on the following link for more information. http://www.ivtusa.com/anatomy Regards. M.Zia Abbas Enersoft. Note: If you don't want to receive future announcements from Enersoft Educational Services, please reply to this message with the word "UNSUBSCRIBE" in the subject. From owner-diagnostics@net.bio.net Tue Aug 17 09:11:00 1999 Path: biosci!biosci!not-for-mail From: Howard S. Judelson [mailto:judelson@ucrac1.ucr.edu] Newsgroups: bionet.diagnostics Subject: POSTDOCTORAL POSITIONS Date: 17 Aug 1999 03:11:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7pbcgr$ac3@net.bio.net> NNTP-Posting-Host: net.bio.net Functional genomics/molecular genetics of the plant pathogen, Phytophthora infestans Positions are available in our laboratory to study the molecular genetics of Phytophthora infestans, the oomycete fungus that causes late blight of potato. P. infestans is a model system for classical and molecular genetic analyses within the oomycetes, and one of the most important plant pathogens. Opportunities exist in several areas: 1. Studying the function of genes that are differentially expressed during sexual and asexual sporulation. 2. Molecular analysis of the mating type locus, including comparisons with homologues in other heterothallics plus homothallic species. 3. Investigating the bases of single and multi-fungicide resistance. These topics are being explored using methods that include positional cloning, subtraction cloning, genomics/EST sequencing, expression profiling using microarrays, manipulating gene expression in P. infestans transformants, etc. The positions are available for a minimum of 2 years, with funding coming from governmental (USDA-NRI, NSF) and industrial sources. Motivated applicants with training in molecular biology will receive highest priority in the search. Experience with filamentous fungi is not required and scientists with training in other systems, such as plants, are encouraged to apply. The laboratory is located at the Riverside campus of the University of California, which is situated in a dynamic region of southern California. Additional information about the laboratory and representative publications are available at: http://cnas.ucr.edu/~ppath/faculty/hj.html. To apply, send a curriculum vitae and the names of three references (including addresses and telephone numbers) to: Dr. Howard Judelson Department of Plant Pathology University of California Riverside, California 92521 USA email: judelson@ucrac1.ucr.edu; fax: 909-787-4294 From owner-diagnostics@net.bio.net Mon Aug 30 07:23:00 1999 Path: biosci!biosci!not-for-mail From: Masoud Bahrami [mailto:mbahram@gwdg.de] Newsgroups: bionet.diagnostics Subject: antibody staining Date: 30 Aug 1999 01:23:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7qdf1j$nfk@net.bio.net> NNTP-Posting-Host: net.bio.net Hi all: I got a problem recently. I have to localise the gfp (green fluorecence protein) in the nucleus of cells from c.elegans with antibody which is conjugated with nanogold. Now I want to ask evryone. Does anyone who have the experience to labeling the gfp in the nucleus with nanogold before? If so, do you have any good ideas on how to optimise and enhance the results. Does anyone know of a worker who could help me out? Or can somebody please point me to a resource or give me some information on each protocol? Thank you in advance, Masoud mbahram@gwdg.de From owner-diagnostics@net.bio.net Mon Aug 30 09:17:00 1999 Path: biosci!biosci!not-for-mail From: art roberts [mailto:arthurr@wsunix.wsu.edu] Newsgroups: bionet.diagnostics Subject: Site with many biology links!!! Date: 30 Aug 1999 03:17:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7qdlnh$8q0@net.bio.net> NNTP-Posting-Host: net.bio.net Great, innovative, and expanding site found at http://www.biotech-resource.com Features -------- Bioethics/ Legal Issues - What are the ethical and legal issues in biotechnology today? Best sites in Biotechnology - It is a list of the Best Biotechnology Sites in the World. Employment opportunities - Employment opportunities in the Biotechnology/Biology field. Search Engine - this simple search engine allows access to all links and the information in the site. Add-A-Link - add your favorite link to my website. This site provides a wide range of information and links for the biochemist, biophysicist, molecular biologist, and science educator. There is also an extensive list of software resources that are free and available over the internet. This site is non-commercial and it is available for your enjoyment. I appreciate your comments and suggestions, and I will reply to all emails (email: arthurr@wsunix.wsu.edu). Coming Soon ----------- This site will soon contain book and software reviews of software that is relevant to biotechnology. I also plan to have stock information available about biotechnology companies. Sincerely, Art Roberts (web designer) (P.S. This site can also be accessed by http://biotech.iscool.net or http://www.ahpcc.unm.edu/~aroberts , if you have trouble accessing it by http://www.biotech-resource.com .)