From owner-diagnostics@net.bio.net Mon Oct 04 07:48:00 1999 Path: biosci!biosci!not-for-mail From: Alex Chang [mailto:lpss@unixg.ubc.ca] Newsgroups: bionet.diagnostics Subject: looking for a good antibody for h. pylori Date: 4 Oct 1999 01:48:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9pkf$8ak@net.bio.net> NNTP-Posting-Host: net.bio.net A friend is looking for a good antibody, preferably rabbit polyclonal, against H. pylori (whole bacteria). If you have any experience, please share. Many thanks, Alex Chang From owner-diagnostics@net.bio.net Mon Oct 04 09:20:00 1999 Path: biosci!biosci!not-for-mail From: stewartl@best.com [mailto:stewartl@best.com] Newsgroups: bionet.diagnostics Subject: PCR: Advantages and Disadvantages of PCR-Based Detection Kits Date: 4 Oct 1999 03:20:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9v14$ge8@net.bio.net> NNTP-Posting-Host: net.bio.net In this month's Alkami PCR Reviews Dr. Michael Zwick discusses the Advantages and Disadvantages of PCR-Based Detection Kits. "This month I conclude my three part series on why I feel PCR-based diagnostics should be common in the clinical lab setting. Last month, I discussed three reasons why PCR is particularly useful, i.e., that it is fast, doesn't require culturing, and is cost-effective. Today I will discuss some additional rationale for considering the use of PCR in the clinical setting.". You can find his article at http://www.alkami.com/reviews/mszver03.htm The Alkami Biosystems site, http://www.alkami.com, is a comprehensive resource for the polymerase chain reaction (PCR). We have online references that include primer design tips and tools, reagent concentrations, polymerase characteristics, PCR methods, and temperature calculations.In addition, you will find regular reviews of PCR issues, employment resources for the biologist, as well as special FREE lab supply offers at http://www.alkami.com/products/idxprods.htm Thanks for your interest. --- ALKAMI BIOSYSTEMS, Inc. Simplified Solutions for Complex Questions (tm) info@alkami.com http://www.alkami.com P.O. Box 11216 Berkeley CA 94712 USA From owner-diagnostics@net.bio.net Mon Oct 04 09:20:00 1999 Path: biosci!biosci!not-for-mail From: stewartl@best.com [mailto:stewartl@best.com] Newsgroups: bionet.diagnostics Subject: High-Throughput Screening Review Date: 4 Oct 1999 03:20:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9v1t$gf0@net.bio.net> NNTP-Posting-Host: net.bio.net This month in Alkami PCR Reviews Robert H. Lee writes the first in his series High-Throughput Screening: Beyond the genomic haystack. "Laboratory scientists have initially and most often implemented high-throughput and ultra-high-throughput screening techniques (HTS and UHTS) in genomics and genomic-based research (e.g., drug discovery). This isn't surprising, as when you're plowing through a human or non-human genome to try to come up with that new wonder drug, you've got a lot of genome to look through-the proverbial needle in the haystack ..."  visit http://www.alkami.com/reviews/hts01.htm to find out more. The Alkami Biosystems site, http://www.alkami.com, is a comprehensive resource for the polymerase chain reaction (PCR). We have online references that include primer design tips and tools, reagent concentrations, polymerase characteristics, PCR methods, and temperature calculations.In addition, you will find regular reviews of PCR issues, employment resources for the biologist, as well as special FREE lab supply offers at http://www.alkami.com/products/idxprods.htm Thanks for your interest. --- ALKAMI BIOSYSTEMS, Inc. Simplified Solutions for Complex Questions (tm) info@alkami.com http://www.alkami.com P.O. Box 11216 Berkeley CA 94712 USA From owner-diagnostics@net.bio.net Mon Oct 04 09:21:00 1999 Path: biosci!biosci!not-for-mail From: stewartl@best.com [mailto:stewartl@best.com] Newsgroups: bionet.diagnostics Subject: PCR Optimization Techniques Date: 4 Oct 1999 03:21:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9v2u$gfh@net.bio.net> NNTP-Posting-Host: net.bio.net This month in Alkami PCR Reviews Dr. Laura Ruth talks about how you can troubleshoot and optimize your laboratory protocols with the powerful and well established techniques of Design of Experiments (DOE) and the Taguchi method at http://www.alkami.com/reviews/rvwoct99.htm The Alkami Biosystems site, http://www.alkami.com, is a comprehensive resource for the polymerase chain reaction (PCR). We have online references that include primer design tips and tools, reagent concentrations, polymerase characteristics, PCR methods, and temperature calculations.In addition, you will find regular reviews of PCR issues, employment resources for the biologist, as well as special FREE lab supply offers at http://www.alkami.com/products/idxprods.htm Thanks for your interest. --- ALKAMI BIOSYSTEMS, Inc. Simplified Solutions for Complex Questions (tm) info@alkami.com http://www.alkami.com P.O. Box 11216 Berkeley CA 94712 USA From owner-diagnostics@net.bio.net Mon Oct 04 09:35:00 1999 Path: biosci!biosci!not-for-mail From: Dave Jensen [mailto:davej@sedona.net] Newsgroups: bionet.diagnostics Subject: Bio Online Career Discussion Forum Date: 4 Oct 1999 03:34:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9vrp$hme@net.bio.net> NNTP-Posting-Host: net.bio.net You are welcome to stop by anytime and review the more than 3500 archived posts related to careers in biotechnology and pharmaceuticals. The Bio Online Career Discussion Forum is a very unique online community. At no other place on the web can you find such interesting discussion threads involving each and every nuance of interest to the job-seeker as well as the professional seeking career guidance and mentoring contacts. Recent topics have included, * How do I get started in a Regulatory Affairs career? * I've got trouble with my Supervisor. How do I handle this kind of situation . . . * Is it wise to pursue my PhD after several years of successful industry employment with my MS? * Dual Career Couples -- What effect on my job search does this really have? You'll find that the Bio Online Career Discussion Forum has a significant readership, and questions are not around long before they have attracted what is usually a number of viewpoints. While the Forum is moderated to keep SPAM and commercial messages to a minimum, it has the fresh, active feeling of a busy coffeehouse. Lots of conversations going on in different corners about a world of subjects, all related to careers in Biotech and Pharmaceuticals. Hope to see you there soon! -- Dave Jensen, Moderator Bio Online Career Discussion Forum http://www.bio.com/hr/forum email to davej@sedona.net From owner-diagnostics@net.bio.net Mon Oct 04 09:37:00 1999 Path: biosci!biosci!not-for-mail From: Andy Zaayenga [mailto:andy.zaayenga@bigfoot.com] Newsgroups: bionet.diagnostics Subject: Announce: Laboratory Robotics Meeting 10/7/99 Date: 4 Oct 1999 03:36:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 251 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7t9vvk$hn8@net.bio.net> NNTP-Posting-Host: net.bio.net The Laboratory Robotics Interest Group Mid Atlantic Chapter October 1999 Meeting High Throughput Screening: Special Topics Date: Thursday, October 7, 1999 Place: Forsgate Country Club, Forsgate Drive, Jamesburg, NJ 08831 Phone: (732)521-0070 Itinerary: Social Period - 3:30 - 6:00 PM Presentations - 6:15 to 8:30 PM Member Pre-Registration: Requested, not required. Pre-registering will allow us to more accurately gauge seating requirements and refreshment needs. Indicate names of attendees and company affiliation. Pre-register by email with or by phone at (732)302-1038. In order to speed sign-in at the meeting, please bring a business card to drop into the registration box. There will be a business card drawing for one of our beautiful LRIG rosewood pens. ************************************************************* Agenda: The theme of the evening is High-Throughput Screening: Special Topics. After the extended social period from 3:30 to 6:00, the talks will commence leading off with Dr. Sheri Miraglia talking about an exciting new development in scanning laser imaging for high-throughput screening applications. Secondly, we will hear Dr. Susan Bassett talk about the problems of interpreting the voluminous data that we get from HTS and some possible software solutions addressing this problem. Finally, Dr. Dale Christensen will talk about a novel approach in finding small molecules that interact with receptors for which ligands are not necessarily known. Taken together these speakers, who are coming far and wide from California, New Mexico, and North Carolina, will ensure a stimulating evening. As always, there is no registration fee or dues, and food and refreshments will be served throughout the social period. Registration is encouraged for us to accurately gauge the size of the meeting. There are hotels nearby for attendees who wish to stay overnight. There will be a Job posting board at the social. Please encourage your recruiters to give you material to post and distribute. Openings may also be posted at http://www.lab-robotics.org/careers.htm Members interested in presenting a scientific poster are encouraged to do so. Please contact us to arrange for poster space. There is no fee to attend the meeting. ************************************************************* Presentation: High-Throughput Screening Applications of a Novel Scanning Laser Imaging Technology Sheri Miraglia, Ph.D., Senior Scientist, PE Biosystems The increasing number of compounds available for screening in drug development has driven the requirement for higher throughput screening technologies, as well as unique technologies that address a broader application portfolio. Our laboratory has been involved in the development of multiplexed mix-and-read assays using Fluorometric Microvolume Assay Technology (FMAT). FMAT is a fluorescence based assay system that incorporates a laser scanner and optical detection system that provides a direct measurement of cellular or bead-based fluorescence on a well-to-well and on an individual cell/bead basis. This design is ideal for the homogenous identification of hits in primary screening, as well as for lead optimization in the form of IC50 determinations, and for assessment of lead compound cytotoxicity. Fluorescent beads of various sizes can be distinguished from one another, allowing the multiplexing of two or more targets present on different sized beads in the same well. In addition, the digitized image data is compiled from two PMTs permitting the development of multiplexed assays based on dye color. A variety of different mix and read applications for FMAT will be described, including peptide-receptor ligand interactions, and multiplexed bead based immunocapture assays. The results of a novel high-throughput screen performed in collaboration with a major Pharma oncology group will also be discussed. Employing a simple mix-and-read Annexin V binding assay, a variety of purified natural products were identified that are potent inducers of apoptosis in tumor cells. Taken together, the data to be presented will demonstrate the versatility and feasibility of fluorescence-based homogeneous and multiplexed assays for a variety of cell-based and molecular targeted screens used in drug discovery. ************************************************************* Presentation: Harnessing the Power of Computational Intelligence to Identify Leads in HTS Susan I. Bassett, Ph.D., Executive Vice-President, Global Technology Operations, Bioreason, Inc. With high-throughput screening systems in place and beginning to produce data reliably, the data analysis and interpretation becomes a bottleneck in the process of moving more high-quality leads to the clinic. The decision-making processes that go into lead discovery, evaluation, and development are quite complex, and can benefit from judicious use of appropriate computational intelligence techniques. Knowledge-based reasoning systems that capture the decision process of a pharmaceutical chemist during lead identification and development and aid in decision support will be presented in this talk. Bioreason's HTS data interpretation systems are an example of an automated solution aimed at helping identify top quality lead candidates while minimizing costly mistakes. The fundamental aspects of technology for combining computational intelligence techniques with knowledge discovery from data mining to this end will be presented. ************************************************************* Presentation: Molecular Braille: A Novel Technology for Identification and Characterization of Compounds that Modulate Receptor Function Dale J. Christensen, PhD., Senior Scientist Novalon Pharmaceutical Corp., 4222 Emperor Blvd., Suite 560 Durham, NC 27703-8466, Ph 919-474-8888 x34, Fax 919-474-0103, dchristensen@novalon.com Many receptor proteins in cellular signaling pathways undergo significant conformational changes in response to a signal molecule. Nuclear hormone receptors are ligand-dependent transcription factors. Ligand binding to these receptors results in conformational changes that expose binding sites for coactivator or corepresser proteins. The estrogen and androgen receptor are well validated drug targets that have a significant clinical utility while orphan nuclear receptors such as PPAR are beginning to play an important role in modern drug discovery. Molecular Braille has been developed to identify and characterize compounds that modulate the conformation of these receptors. Using a series of conformation-sensitive probes, the conformation that the receptor adopts in response to ligand binding can be evaluated using an in vitro time-resolved fluorescence assay and an in vivo luminescence assay. This technology can be used to identify new ligands, distinguish between classes of ligands, and guide lead optimization. ************************************************************* For more information, contact one of the LRIG Mid Atlantic officers: Dennis S. France Executive Chair, LRIG Home and Mid Atlantic Chapter The Novartis Institute for Biomedical Research tel: (908) 277-5328 fax: (908) 277-4374 email: dennis.france@pharma.novartis.com John Babiak, Ph.D. High Throughput Screening Chair, Mid Atlantic Chapter Pharmacopeia tel: (609) 452-3795 fax: (732) 821-2037 email: jbabiak@pharmacop.com William Haller Analytical Chemistry Chair, Mid Atlantic Chapter Treasurer, LRIG Home and Mid Atlantic Chapter Ortho-McNeil Pharmaceutical tel: (908) 218-6341 fax: (908) 218-0524 email: bill.haller@lab-robotics.org Sharon Reed Event Coordinator, Mid Atlantic Chapter Coelacanth Corporation tel: (609) 448-8200 ext 2044 email: sharon.reed@lab-robotics.org M. Elizabeth Miller Agricultural Applications Chair, Mid Atlantic Chapter Rohm & Haas Company tel: (215) 641-7285 fax: (215) 619-1617 email: rsaumm@rohmhaas.com Jinzi Wu, Ph.D. Assay Development Chair, Mid Atlantic Chapter The Novartis Institute for Biomedical Research email: jinzi.wu@pharma.novartis.com Andy Zaayenga Automation Technologies Chair, Mid Atlantic Chapter Secretary, LRIG Home and Mid Atlantic Chapter TekCel Corporation tel: (732) 302-1038 fax: (732) 302-9080, eFax: (630) 604-2935 email: andy.zaayenga@tekcel.com ************************************************************* Directions: From the North or South: Take the New Jersey Turnpike to Exit 8A - Exit the left ramp for Jamesburg (Route 32 East) - Continue straight for 1 1/4 miles through traffic light - Forsgate Country Club is on your left - Use the Clubhouse Entrance (second left). From Princeton: Route 1 to Scudders Mill Road East - Continue on Scudders Mill Road and make a left at the 5th traffic light onto Dey Road - Continue on Dey Road to the end - Make a left - At 2nd traffic light (Route 32) make a right - Continue straight for 1 1/4 miles through traffic light - Forsgate Country Club is on your left - Use the Clubhouse entrance (second left). ************************************************************* For more information on this meeting, visit http://www.lab-robotics.org/Mid_Atlantic/meetings/9910.htm Visit The Laboratory Robotics Interest Group homepage at http://lab-robotics.org ************************************************************* The Laboratory Robotics Interest Group is a rapidly growing special interest group focused on robotics applications in the laboratory. Our membership consists of over 5,000 scientists and engineers worldwide with more than 2,800 in the Mid Atlantic Chapter. We are a non-profit organization run by unpaid volunteers so if you would like to help out with the group please contact one of our officers. From owner-diagnostics@net.bio.net Mon Oct 04 09:40:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator [mailto:biohelp@net.bio.net] Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 4 Oct 1999 03:39:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 241 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7ta05d$i3n@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-AUG-99) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- All BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- the UK-HGMP-Resource Centre (known as hgmp.mrc.ac.uk): ----------------------------------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@hgmp.mrc.ac.uk. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to majordomo@hgmp.mrc.ac.uk. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Please ask for help at biosci@hgmp.mrc.ac.uk if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnostics@net.bio.net Tue Oct 05 09:55:00 1999 Path: biosci!biosci!not-for-mail From: Kevin O'Donnell [mailto:Kevin.ODonnell@sasa.gov.uk] Newsgroups: bionet.diagnostics Subject: IRRI Plant Pathologist - Bangladesh Date: 5 Oct 1999 03:55:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 85 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7tclej$9hm@net.bio.net> NNTP-Posting-Host: net.bio.net Position of Technical Adviser in Plant Pathology Terms of Reference for Technical Adviser Pathologist, PETRRA Seed Health Improvement Component. Job Description: Position Announcement for Affiliate Scientist (Plant Pathologist) The International Rice Research Institute (IRRI) is seeking an affiliate scientist (plant pathologist) under a study on seed health improvement for farmer-saved rice seed, a component of the project "Poverty Elimination Through Rice Research Assistance (PETRRA) funded by the Department for International Development, UK. The position, to be based in Bangladesh, is offered for 2 years, with the possible opportunity of subsequent employment for a further period in support of the project. IRRI, supported by the Consultative Group on International Agricultural Research, (CGlAR) is a non-profit, autonomous organization, engaged in research and training on rice-related technology. Interested candidates can learn more about IRRI and its activities by visiting the Institute's Website at http:/www.cgiar.org/irri. The Affiliate Scientist will work closely with and provide technical advice to the Team Leader for the Seed Health Improvement Component of PETRRA, involving an interdisciplinary team of scientists from Bangladesh Rice Research Institute (BRRI) and associated institutions on the following main activities: 1. Assist the installation of PCR and other laboratory equipment and its operational establishment at the Plant Pathology laboratory of BRRI. Provide hands-on training to BRRI scientists in the use and applications of molecular tools. 2. Support research programmes assessing the significance of seed health under farmers conditions and utilizing molecular biological tools to assess the significance of seed as a source of field inoculum. With BRRI staff, further develop PCR techniques to identify and discriminate between species present. 3. Assist the prioritization, planning and implementation of a detailed programme of farmer-participatory research to improve seed health at farm level, based on measures appropriate to the socio-economic status of Bangladesh farmers. 4. Assist BRRI management in the identification of suitable candidates and research topics for postgraduate training programmes complementary to the project theme and assist supervision of these students as appropriate. 5. Ensure that BRRI scientists are equipped with the necessary skills and resources to be able to undertake subsequent laboratory and field-based programmes, provide guidelines to BRRI management on how the facilities and expertise at BRRI may best be used in the future programmes. 6. Provide support to PETRRA Project Manager in matters related to rice seed health for the Project operation. Applicants should have a PhD in plant pathology with statistical skills and experience of molecular biology. Research experience in disease management and experience of working in inter-disciplinary approaches in a developing country is highly desirable. Good communication and inter-personal skills are important for work with colleagues in the national agricultural research system and extension organizations. The affiliate scientist will be located in Dhaka, Bangladesh. He or she will report through the IRRI Representative for Bangladesh to the Project Coordinator/Head of Entomology & Plant Pathology Division, IRRI. Salary and perquisites are internationally competitive. Send comprehensive curriculum vitae and names, addresses and fax numbers of three referees to: Dr M. Holderness CABI Bioscience Bakeham Lane, Egham, Surrey TW20 9TY UK Tel: 01491 829043 Fax: 0491 829100 e-mail: m.holderness@cabi.org (CABI Bioscience are providing coordination of UK inputs to the project) From owner-diagnostics@net.bio.net Tue Oct 05 10:11:00 1999 Path: biosci!biosci!not-for-mail From: Sergey Kosourov [mailto:sergks@issp.serpukhov.su] Newsgroups: bionet.diagnostics Subject: BioScience Links Date: 5 Oct 1999 04:09:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7tcm8p$anj@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleagues, We are glad to announce the new information resource - BioScience Links - has been developed on the basis of the Laboratory of Biochemistry and Biotechnology of Phototrophic Microorganisms web-server (Institute of Basic Biological Problems, RAS; Pushchino). At the moment, BSL database contains 154 links related to biological studies. Biochemistry, biotechnology, molecular biology, software, journals and other links are presented. We hope that this resource might be useful to you. You can find it at http://www.biolinks.net.ru Please, send us any your comments. Best regards, Webmaster From owner-diagnostics@net.bio.net Thu Oct 07 09:17:00 1999 Path: biosci!biosci!not-for-mail From: tritty [mailto:tritty@cellbio.wustl.edu] Newsgroups: bionet.diagnostics Subject: micoplasma kits? Date: 7 Oct 1999 03:17:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7thrvs$nu6@net.bio.net> NNTP-Posting-Host: net.bio.net can anyone recommend a kit for the detection of micoplasma in tissue culture reagents? thanks in advance, Tim ------------------------------------------------------------------------ ---- ---------------------- Timothy M. Ritty, Ph.D. (314) 362-2211 Washington University Dept. of Cell Biology, Box 8228 660 South Euclid St. Louis, MO 63110 From owner-diagnostics@net.bio.net Thu Oct 07 09:36:00 1999 Path: biosci!biosci!not-for-mail From: Robert Burns [mailto:burns@sasa.gov.uk] Newsgroups: bionet.diagnostics Subject: RE: micoplasma kits? Date: 7 Oct 1999 03:36:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 57 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7tht33$q7e@net.bio.net> NNTP-Posting-Host: net.bio.net Hi Tim, we have been using the Boehringer Mycoplasma Detection kit for a number of years to ensure that our hybridoma stocks are mycoplasma free. The test is based on a DAS avidin/biotin ELISA of tissue culture supernatant and is quick and easy to use. We messed about with the Life Technologies Mycotect kit which uses an indicator cell line (3T3) which is sensitive to methylpurine and methylpurine riboside. This product is converted from non-toxic methyl purine deoxy riboside by the action of adenosine phosphorolase (produced by mycoplasma). The test takes 5-6 days against 1 day for the Boehringer system. It's main advantage is that it will detect all mycoplasma instead of the "big four" that the Boehringer system identifies. We also tried the Hoechst mycoplasma stain system but found it very difficult to interpret as you are basically looking for tiny amounts of extra-nuclear mycoplasma DNA (which stain positive with the dye). Hope this is of use to you Regards Robert -----Original Message----- From: tritty [mailto:tritty@cellbio.wustl.edu] Sent: 06 October 1999 21:21 To: bionet-diagnostics@net.bio.net Subject: micoplasma kits? can anyone recommend a kit for the detection of micoplasma in tissue culture reagents? thanks in advance, Tim ------------------------------------------------------------------------ ---- ---------------------- Timothy M. Ritty, Ph.D. (314) 362-2211 Washington University Dept. of Cell Biology, Box 8228 660 South Euclid St. Louis, MO 63110 From owner-diagnostics@net.bio.net Tue Oct 12 07:34:00 1999 Path: biosci!biosci!not-for-mail From: Marcel Dinger [mailto:marcel@genamics.com] Newsgroups: bionet.diagnostics Subject: Genamics JournalSeek 93258 Date: 12 Oct 1999 01:34:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7turq6$fsh@net.bio.net> NNTP-Posting-Host: net.bio.net GENAMICS JOURNALSEEK The largest journal information database on Earth. Check it out now at: http://genamics.com/journals/ Genamics JournalSeek is a fully searchable database of journal information. JournalSeek now contains over 8500 journal titles. Information includes abbreviated titles, aims and scope, publisher, link to online site, availability of abstracts/full-text online, ISSNs, and journal impact factors. All information is freely provided. Marcel Dinger, Genamics. http://genamics.com zs From owner-diagnostics@net.bio.net Mon Oct 25 09:12:00 1999 Path: biosci!biosci!not-for-mail From: news@brainiac.rz.uni-ulm.de [mailto:news@brainiac.rz.uni-ulm.de] Newsgroups: bionet.diagnostics Subject: S: Cell Tracker Orange Date: 25 Oct 1999 03:12:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7v1ae4$2gc@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, could anyone point me a source, description or just information about "Cell Tracker TM Orange". Please follow up per email only. Thank you. -- Dr. Jörg Fiedler Abt. für Polyklinik und Orthopädie Sektion Biochemie Universitätsklinik Ulm 89081 Ulm Tel.: 0731-502 3795 Fax: 0731-502 3797 From owner-diagnostics@net.bio.net Tue Oct 26 07:40:00 1999 Path: biosci!biosci!not-for-mail From: news@brainiac.rz.uni-ulm.de [mailto:news@brainiac.rz.uni-ulm.de] Newsgroups: bionet.diagnostics Subject: S: Cell Tracker Orange Date: 26 Oct 1999 01:40:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7v3pdn$fos@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, could anyone point me a source, description or just information about "Cell Tracker TM Orange". Please follow up per email only. Thank you. -- Dr. Jörg Fiedler Abt. für Polyklinik und Orthopädie Sektion Biochemie Universitätsklinik Ulm 89081 Ulm Tel.: 0731-502 3795 Fax: 0731-502 3797 From owner-diagnostics@net.bio.net Wed Oct 27 14:09:00 1999 Path: biosci!biosci!not-for-mail From: Mikhail Edelstein [mailto:me@casus.cliph.keytown.com] Newsgroups: bionet.diagnostics Subject: Microbial DNA isolation from WBC with Chelex 100? Date: 27 Oct 1999 08:09:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7v74je$onl@net.bio.net> NNTP-Posting-Host: net.bio.net I am looking for a method of microbial DNA extraction from white cell fraction of blood using the Chelex 100 resine. I am particularly interested in isolation of C.pneumoniae DNA with Chelex 100 for subsequent detection by PCR. However, I would appreciate any reply from those who use Chelex for processing PCR blood samples. Sincerely, Michael Edelstein. mailto: me@cliph.keytown.com From owner-diagnostics@net.bio.net Thu Oct 28 07:10:00 1999 Path: biosci!biosci!not-for-mail From: Henrry Bernaola Moreno [mailto:citogenetica@nazcanet.com] Newsgroups: bionet.diagnostics Subject: chromosome finding Date: 28 Oct 1999 01:10:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: odonnell@sasa.gov.uk Distribution: world Message-ID: <7v90e4$49q@net.bio.net> NNTP-Posting-Host: net.bio.net we found trisomy 16 and double minute in an apparently benign giant tumor cell of bone of the hand of 35years old female patient. We like to known the significance of this alteration ,prognosis significance ____________________________________________________________________ Obtenga su e-mail gratis para toda la vida en http://www.nazcanet.com