From owner-diagnost@hgmp.mrc.ac.uk Fri Dec 3 12:08:14 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7037117A43; Fri, 3 Dec 1999 12:08:12 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id MAA00779 for ; Fri, 3 Dec 1999 12:08:09 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id MAA21502 for diagnost-list@hgmp.mrc.ac.uk; Fri, 3 Dec 1999 12:08:08 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: biohelp@net.bio.net] X-Newsgroups: bionet.diagnostics Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 3 Dec 1999 04:09:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 241 Distribution: world Message-ID: <828bu5$eoo@net.bio.net> To: diagnost@hgmp.mrc.ac.uk Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk (LAST REVISION: 14-AUG-99) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- All BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- the UK-HGMP-Resource Centre (known as hgmp.mrc.ac.uk): ----------------------------------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@hgmp.mrc.ac.uk. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to majordomo@hgmp.mrc.ac.uk. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Please ask for help at biosci@hgmp.mrc.ac.uk if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-diagnost@hgmp.mrc.ac.uk Mon Dec 6 09:12:07 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id BD03317A5B; Mon, 6 Dec 1999 09:12:06 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id JAA26865 for ; Mon, 6 Dec 1999 09:12:04 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA06102 for diagnost-list@hgmp.mrc.ac.uk; Mon, 6 Dec 1999 09:12:03 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: andy.zaayenga@bigfoot.com] X-Newsgroups: bionet.diagnostics Subject: Announce: Laboratory Robotics Meetings Date: 6 Dec 1999 01:17:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 321 Distribution: world Message-ID: <82fuur$pte@net.bio.net> To: diagnost@hgmp.mrc.ac.uk Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk This notice contains information about the next meetings for LRIG Mid Atlantic, LRIG San Diego, LRIG SouthEast, LRIG Bay Area, and LRIG New England. **************************************** The Laboratory Robotics Interest Group Mid Atlantic Chapter December 1999 Meeting High Throughput Screening Using the 1536-Well Format: Distinguishing Fact from Fiction Date: Tuesday, December 7, 1999 Place: Hanover Marriott,1401 Rt 10 E, Whippany, NJ 07981 Phone: 973-538-8811, Fax: 973-538-0291 Itinerary: Social Period & Exhibition - 3:30 to 6:30 PM Presentations - 6:30 to 8:00 PM Panel Discussion - 8:00 to 9:00 PM Registration: Requested, not required. Pre-registering will allow us to more accurately gauge seating requirements and refreshment needs. Indicate names of attendees and company affiliation. Pre-register by email with or by phone at (732)302-1038. In order to speed sign-in at the meeting, please bring a business card to drop into the registration box. There will be a business card drawing for a PalmPilot IIIx donated by TekCel, www.tekcel.com. Agenda: There will be a variety of vendors available exhibiting the latest developments in laboratory automation hardware and software related to 1536-well and higher density formats during a social period (3:30 P.M. - 6:30 P.M.). Food and refreshments will be available, provided by LJL Biosystems, www.ljlbio.com. This will be followed by three talks and a panel discussion. Members interested in presenting a poster are encouraged to do so. Open career positions at your company may be announced or posted. There is no registration or fee associated with this LRIG function. --------------------------------------- Exhibitors: Amersham Pharmacia Biotech Beckman Coulter Becton Dickinson BioSignal Cardinal Instrument Cellomics Corning CRS EMAX Solution Partners Genevac Greiner America IDBS LabVantage Solutions LJL BioSystems Marsh MJ Research, Inc. Modern Drug Discovery Molecular Devices Nalge Nunc International Oyster Bay Pump Works Packard Perkin Elmer Biosystems (Tropix division) Perkin Elmer Wallac Pierce Chemical Popper and Sons Robbins Robocon Tecan Tomtec Zymark --------------------------------------- Presentation: The Complete Solution to ultra-High Throughput Screening Assay Miniaturization. David A. Dunn; Senior Research Fellow; Pharmacopeia, Inc. A complete solution to the challenges of assay development in miniaturized format, including plate miniaturization, liquid handling, optical detection, data management and assay development, is required in order to implement a viable uHTS program. This talk will cover new tools, developed at Pharmacopeia and elsewhere, that address these challenges. Examples of their successful implementation as part of our regular screening operation will be given. --------------------------------------- Presentation: An Ultra High-Throughput Approach for an Adenine Transferase Using Fluorescence Polarization Dr. Julie Li, Hoechst Marion Roussel We have developed a novel assay for measuring the activity of an enzyme that transfers multiple adenine-containing groups to an acceptor protein. The assay is based on fluorescence polarization (FP) technology in a 1536-well plate format. In the assay, a long wavelength fluorescence tracer, Texas Red (Rhodamine), was covalently conjugated to adenine of the donor substrate through a C6 spacer arm. As a result of the transfer of the adenine-containing moieties to the acceptor protein substrate, the rotation correlation time of the Texas Red conjugate increased, and hence the degree of fluorescence polarization. The pharmacological profile and kinetics of the enzyme measured according to the FP method were consistent with those determined previously by conventional analysis. We have successfully executed a 250,000 compound high throughput screening program based on the FP assay method. The quality and validity of the assay were verified by a variety of statistical analyses. --------------------------------------- Presentation: Analysis of Protein-Peptide Interaction by a Miniaturized Fluorescence Polarization Assay Using Cyclin Dependent Kinase 2/Cyclin E as a Model System Ilona Kariv, Sokhom S. Pin, and Kevin R. Oldenburg Leads Discovery, DuPont Pharmaceuticals, Wilmington, DE 19880 As a result of the increasing size of chemical libraries, more rapid and highly sensitive strategies are needed to accelerate the process of drug discovery without increasing the cost. One means of accomplishing this is to miniaturize the assays that enter high throughput screening (HTS). Miniaturization requires an assay design that has few steps, has a large degree of separation between the signal and background, and has a low well to well signal variation. Fluorescence polarization (FP) is an assay type that, in many cases, meets all of the above requirements. FP is a homogenous method that allows interactions between molecules to be measured directly in solution. This article demonstrates the application of FP to a miniaturized HTS format, using 1536-well plates, to measure direct binding between cyclin dependent kinase 2/cyclin E complex (CDK2/E) and an 8-mer-peptide kinase inhibitor. The data indicate that low variability and high specificity allow rapid and precise identification of antagonist compounds affecting CDK2/E-peptide interactions. --------------------------------------- Panel: Following the talks, there will be a moderated panel discussion with HTS experts who are using 1536-well and higher density formats in their laboratories. Panel members include: David A. Dunn, Senior Research Fellow; Pharmacopeia Dr. Julie Li, Hoechst Marion Roussel Ilona Kariv, Leads Discovery, DuPont Pharmaceuticals Daniel Chelsky, BioSignal Jonathan Burbaum, Associate Director, Technology Business Development, Pharmacopeia Meng Zhang, Applications Scientist, LJL --------------------------------------- Poster: Fluorescence Detection Strategies for Electroseparations in Plastic Micro-fabricated Devices Shau-Chun Paul Wang, Department of Chemistry, University of Michigan, scwang@umich.edu Fluorescence background interference from the device is inherent in plastic microchips, particularly with blue or UV excitation. Conventionally, microchip background has been reduced with confocal optics or circumvented with specialized long wavelength fluorophores. We show that microchip background can be rejected with analyte velocity modulation. In this scheme the driving voltage is modulated at low frequency. Migration velocities and analyte signals are modulated at the same frequency. Microchip fluorescence is unmodulated, so that lock-in detection (synchronous demodulation) easily separates the analyte signal from background. The technique does not require a laser source. In our implementation a blue (485nm) LED is the light source. Simple optics are used to shape the source and focus it to a spot about 50 microns in diameter inside a microchip. Photomultiplier detection is employed and a lock-in amplifier is used to demodulate the signal. Apertures in the system generate a derivative response, which can be converted to conventional bands by integration. Fluorescence rejection provided by our first generation system lowers detection limits by five to eight fold compared to DC measurements with the same optical train. --------------------------------------- Poster: Corning 1536-Well Assay Plate for HTS Arthur Trombley, Corning Inc. Science Products Division, Portsmouth, NH USA Jill Veilleux, Corning Inc. Science Products Division, Acton, MA USA David Dunn, Marc Orlowski, Pharmacopeia, Inc., Princeton, NJ USA Meng Zhang, Doug Boyd, LJL Biosystems, Inc., Sunnyvale, CA USA The new Corning 1536-well 2 l Plate provides the foundation for a unique solution to assay miniaturization for high throughput screening. The advantages of the 1536-well HTS plate include: Conservation of 96 and 384 well format, which aids in sample transfer, well identification, and data manipulation Low profile, which provides increased sample density over conventional plate height and reduces parallax effects in imaging systems Three point positioning and tight manufacturing tolerances for all dimensions, which enable high-speed automated plate handling functions The lowest volume wells (2 l) that are commercially available Optimized materials for manufacture, which enhance performance in Fluorescence, luminescence and colorimetric detection formats. Here, we provide an analysis of evaporative loss and automated dispensing at low volumes. The Corning 1536-well 2 l Plate is shown to be an ideal solution to scale up from traditional 96 and 384 well formats in high throughput --------------------------------------- Posters will also be presented by BioSignal and Perkin Elmer Tropix. --------------------------------------- Directions: (on line directions at http://marriotthotels.com/EWRHO/): Take Route 287 to Exit 39 (Route 10 West). Go through one stoplight and take 1st u-turn. --------------------------------------- Detailed information may be found at http//www.lab-robotics.org/Mid_Atlantic/meetings/9912.htm **************************************** The Laboratory Robotics Interest Group San Diego Chapter Tuesday, December 7th, 630 - 930pm. Held in Garren Auditorium, Basic Science Building, UCSD Medical School, La Jolla Campus. Featuring presentations on the following Tentative - Robots and DNA Mass Array Qualification - James E. Kahelin. Tentative - Discovery, Selection, and Early-Stage Development of Novel Small Molecule Drug Therapies - Kia Motesharei, Ph.D., Manager, New Technologies, Trega Biosciences, Inc. Automated Synthesizer utilizing Tilted Plate Centrifugation - Michal Lebl, Spyder Instruments, Inc. Detailed information may be found at http//www.lab-robotics.org/San_Diego/ **************************************** The Laboratory Robotics Interest Group Bay Area Chapter Tuesday, December 7th, 630 - 930pm. Detailed information may be found at http://www.lab-robotics.org/Bay_Area/ **************************************** The Laboratory Robotics Interest Group New England Chapter Next meeting is March 8, 2000 The topic is HTS: A Reality Check Detailed information will be posted shortly to http//lab-robotics.org/New_England/index.html **************************************** The next meeting of the SouthEast Chapter of the Laboratory Robotics Interest Group (LRIG) is set for Friday, February 4, 2000 from 12 noon to 6 p.m. at the Sheraton Imperial in Research Triangle Park, North Carolina. The meeting will consist of a vendor show and vendor presentations. Detailed information may be found at http://www.lab-robotics.org/SouthEast/Feb4_2000.htm **************************************** The Laboratory Robotics Interest Group is a rapidly growing special interest group focused on robotics applications in the laboratory. Our membership consists of over 5,000 scientists and engineers worldwide. We are a non-profit organization run by unpaid volunteers for the benefit of the Laboratory Automation Community. Please visit our web site at http//www.lab-robotics.org/ From owner-diagnost@hgmp.mrc.ac.uk Mon Dec 6 09:38:13 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 2AA1317A5B; Mon, 6 Dec 1999 09:38:12 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id JAA28513 for ; Mon, 6 Dec 1999 09:38:11 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA06467 for diagnost-list@hgmp.mrc.ac.uk; Mon, 6 Dec 1999 09:38:10 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: bettenz@my-deja.com] X-Newsgroups: bionet.diagnostics Subject: BIOTECHNOLOGY in EUROPE Date: 6 Dec 1999 01:18:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Distribution: world Message-ID: <82fv1g$q5b@net.bio.net> To: diagnost@hgmp.mrc.ac.uk Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk I want to start my own biotechnology/microbiology company. I am looking for partners in Europe (preferably in Portugal or Spain). Please feel free to contact me. Thank you very much. bettenz@my-deja.com Sent via Deja.com http://www.deja.com/ Before you buy. From owner-diagnost@hgmp.mrc.ac.uk Tue Dec 7 11:15:21 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id F137F17A43; Tue, 7 Dec 1999 11:15:20 +0000 (GMT) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id LAA06490 for ; Tue, 7 Dec 1999 11:15:19 GMT Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id LAA03785 for diagnost-list@hgmp.mrc.ac.uk; Tue, 7 Dec 1999 11:15:17 GMT X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: tigergil@aol.com] X-Newsgroups: bionet.diagnostics Subject: a new edition of the gazette Date: 7 Dec 1999 02:53:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Distribution: world Message-ID: <82iovl$750@net.bio.net> To: diagnost@hgmp.mrc.ac.uk Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk see http://members.aol.com/ageingpath/geriatricpathologygazette for a new edition...tigergil@aol.com tigergil@aol.com From owner-diagnost@hgmp.mrc.ac.uk Mon Dec 13 09:20:28 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id C8BC017AD0; Mon, 13 Dec 1999 09:20:27 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id 6318B17ABC; Mon, 13 Dec 1999 09:20:26 +0000 (GMT) To: nobody@net.bio.net X-Really-To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: daemon@hgmp.mrc.ac.uk, [mailto:daemon@hgmp.mrc.ac.uk]@hgmp.mrc.ac.uk, On@hgmp.mrc.ac.uk, Behalf@hgmp.mrc.ac.uk, Of@hgmp.mrc.ac.uk Subject: ANNC: MICROARRAY USER SURVEY Message-Id: <19991213092026.6318B17ABC@mercury.hgmp.mrc.ac.uk> Date: Mon, 13 Dec 1999 09:20:26 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk To all investigators involved in microarray technologies: The 1999/2000 ABRF MICROARRAY RESEARCH GROUP STUDY is open for submissions as of December 10, 1999. The goal of this study is to build a current picture of the microarray analysis world. Microarray analysis is a fast developing field. New methods and instrumentation are being constantly introduced and the number of investigators using the technology is rapidly expanding. We are focusing on both Affymetrix and cDNA related technologies. Laboratories using these technologies can participate in our study by filling out a web based survey form. The form is used to collect information such as instrumentation, protocols, staffing, funding, and throughput. This data will be analyzed to build a current profile of microarray analysis laboratories. The survey from is posted at http://brcweb.bio.cornell.edu/surveys/microsurvey.html. The survey is geared to gathering information from academic, pharmaceutical, and commercial laboratories that offer microarray technologies as a shared resource. Individual laboratories that have these technologies are also welcome to participate in the survey. In addition, microarray instrument and related manufacturers are welcome to submit data. However, data from manufacturers must be clearly identified and will be analyzed separately from the pool of data submitted anonymously by all other laboratories. An analysis of data submitted to this survey will be presented at the ABRF2000 meeting in February 2000 (http://www.faseb.org/meetings/abrf2000) and will then be posted on the web. We appreciate your participation in this study. The ABRF Microarray Research Group: George Grills, Albert Einstein College of Medicine, Bronx, NY (chair) Chandi Griffin, Gen. Clin. Res. Ctr., University of California, San Francisco,CA Kathryn Lilley, University of Leicester, UK Aldo Massimi, Albert Einstein College of Medicine, Bronx, NY Yongde Bao, University of Virginia School of Medicine, Charlottesville, VA (ad hoc) Suzanne Sandmeyer, University of California, Irvine, CA (ad hoc) James VanEe, Cornell University, Ithaca, NY (ad hoc) ------------------------------------------------------------------ Please direct questions about participation in this study to George Grills at grills@aecom.yu.edu Chandi Griffin GCRC: Molecular Biology Core Laboratory & GCRC GeneChip Contact Univ. of California, San Francisco/San Francisco General Hospital E-mail: aniya@itsa.ucsf.edu --- From owner-diagnost@hgmp.mrc.ac.uk Mon Dec 20 15:31:06 1999 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 0649317AD4; Mon, 20 Dec 1999 15:31:05 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 1) id B4E7117AD3; Mon, 20 Dec 1999 15:31:04 +0000 (GMT) To: nobody@net.bio.net X-Really-To: diagnost@net.bio.net Newsgroups: bionet.diagnostics From: daemon@hgmp.mrc.ac.uk, [mailto:daemon@hgmp.mrc.ac.uk]@hgmp.mrc.ac.uk, On@hgmp.mrc.ac.uk, Behalf@hgmp.mrc.ac.uk, Of@hgmp.mrc.ac.uk Subject: FW: Potato Virus X transmission Message-Id: <19991220153104.B4E7117AD3@mercury.hgmp.mrc.ac.uk> Date: Mon, 20 Dec 1999 15:31:04 +0000 (GMT) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk > -----Original Message----- > From: Isla Browning > Sent: Monday, December 20, 1999 3:13 PM > To: 'diagnost@net.bionet' > Subject: Potato Virus X transmission > > I would be interested to hear of any evidence of transmission of PVX > within crops by aphids. > > Many thanks > > Isla Browning ---